Rh5AG277500

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004011
Physical Location & Seq
Forward (+)
37316645 .. 37322573
5929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG277500.1

Sequence Viewer

Length: 1314 bp
ATGGGAGACAATGGGGATGCCCTGTTAAACCGAAAGAGCAACAAGTTCCAGCGCTGCGTTTCACATACACATGATGAATTGCAGATCTTTAGATCCTACCTGCGATGGATGTTCGTGGATCAATCCAATGTTTGGAGAGCATGTCTCTCTTGGTGCATGTTCATTCTCTTTGGAATCGTTGTCCCCGCCATATCCCATTTCGTGTTTGCCTGCGCCACCTGCGACGGAAACCACAAGAGGCCCTATGACAGTGTCGTGGAATTGTCATTGAGCAGCGTCGCGACGGTGTCATTTGTGTGCCTCTCATGGTTTGTAAGGAAGTTTGGGCTCCGAAGGTTCTTGTTTTTTGATAAGCTTTATTATGAGAGCGAAACCGTCAGGAGGGAATATACAGCACAATTAAATAGGTCATTGAAAATTCTCTCCATCTTTGTCCTACCATGCTTTTTTGCCGAAAGTGTGTATAAGATATGGTGGTACACTTCGGGATTCTCACAAGTGCCTTTCGTGGCTAATGTTTACGTAAGTTCCACTGTAGCATGCATTCTAGAACTGTGTTCATGGCTCTACCGGACGACCGTGTTTTTCCTCGTGTGTGTTCTGTTTCGGTTGATTTGCTACCTCCAAATCTTAAGGCTTCAAGACTTTGCCACAGTGTTTCAAGTCGAATCCGACGTGGCGTCAATTATGTCCGAGCACCTTAGAATCCGAAGGCACTTAACGATCATAAGCCATAGATTCCGGGGGTTCATATTGTGGTCTCTAACATTGATCACAGGGAGCCAATTCGCATTGTTGCTCATCACGACCAAGTCCAGTGCCGCTGTCGATTATACGCATATTTTCAGAGTTGGGGAACTTGTGTTATGCTCCATAACTTTGGTTACCGGCCTCCTCCTCTTACTCCGCAGCGCAACTAAAATAACACACAAGGCCCAAGCAGTGGCATGCCTAGCTACCAAGTGGCATGTTTGTGCCACACTGGAGTCGTTTGATGGAACCGAGTTGGATTCTCCAACGGCTCAAATTGCGAATGATCCAGCATTTTATTTTAACACCGACGAAGAATCAGACGGGGATTTGGTTAGTGATGAAGATAATGACTTGGAAAACACCAAGATGGTGCCATCTTGTTCATCTCATACCATTTCTTACCAAAAACGTCAAGCTCTGGTGACCTATTTTGAGAACAATAAAGCCGGCATTACTGTTTACGGGTTCACGTTGGATAGGACTACACTGCACATGTTATTTATGATTGAATTTTCATTAATTCTCTGGCTGTTGGGGAAGACCATTAATGGAATTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

437

Amino Acids

50.08

Weight (kDa)

7.52

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 25 - 420 8.6e-167 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 227
Acc36I ACCTGC 2 cut(s) 108, 227
AccB1I GGYRCC 1 cut(s) 1123
AccB7I CCANNNNNTGG 2 cut(s) 132, 943
AccII CGCG 1 cut(s) 281
AciI CCGC 3 cut(s) 186, 822, 907
AclWI GGATC 3 cut(s) 87, 126, 1031
AcsI RAATTY 3 cut(s) 417, 1262, 1305
AcyI GRCGYC 1 cut(s) 680
AfaI GTAC 1 cut(s) 479
AfeI AGCGCT 1 cut(s) 53
AfiI CCNNNNNNNGG 3 cut(s) 132, 381, 943
AflII CTTAAG 1 cut(s) 631
AflIII ACRYGT 1 cut(s) 1245
AgsI TTSAA 4 cut(s) 415, 641, 662, 1262
AhdI GACNNNNNGTC 1 cut(s) 679
AjiI CACGTC 1 cut(s) 676
AluBI AGCT 3 cut(s) 355, 956, 1169
AluI AGCT 3 cut(s) 355, 956, 1169
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 2 cut(s) 149, 765
AlwI GGATC 3 cut(s) 87, 126, 1031
Aor51HI AGCGCT 1 cut(s) 53
AoxI GGCC 3 cut(s) 239, 889, 933
ApeKI GCWGC 3 cut(s) 54, 273, 909
ApoI RAATTY 3 cut(s) 417, 1262, 1305
AseI ATTAAT 2 cut(s) 1271, 1299
AspLEI GCGC 3 cut(s) 54, 215, 914
AspS9I GGNCC 2 cut(s) 240, 934
AsuC2I CCSGG 1 cut(s) 743
AsuHPI GGTGA 1 cut(s) 1186
BanI GGYRCC 1 cut(s) 1123
BanII GRGCYC 1 cut(s) 330
BauI CACGAG 1 cut(s) 590
BbsI GAAGAC 1 cut(s) 1298
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 3 cut(s) 41, 285, 921
BccI CCATC 5 cut(s) 99, 434, 989, 1114, 1135
BceAI ACGGC 1 cut(s) 1035
BclI TGATCA 1 cut(s) 771
BcnI CCSGG 1 cut(s) 743
BcoDI GTCTC 2 cut(s) 149, 765
BfaI CTAG 2 cut(s) 548, 953
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 55
BfrI CTTAAG 1 cut(s) 631
BfuAI ACCTGC 2 cut(s) 108, 227
BglII AGATCT 1 cut(s) 84
BisI GCNGC 4 cut(s) 55, 274, 822, 910
BlsI GCNGC 4 cut(s) 56, 275, 823, 911
Bme1390I CCNGG 1 cut(s) 743
BmeRI GACNNNNNGTC 1 cut(s) 679
BmgBI CACGTC 1 cut(s) 676
BmgT120I GGNCC 2 cut(s) 240, 934
BmiI GGNNCC 4 cut(s) 329, 782, 1000, 1125
BmrFI CCNGG 1 cut(s) 743
BmsI GCATC 1 cut(s) 7
BpiI GAAGAC 1 cut(s) 1298
BplI GAGNNNNNCTC 2 cut(s) 129, 161
BpmI CTGGAG 1 cut(s) 1004
BpuMI CCSGG 1 cut(s) 743
BsaAI YACGTR 1 cut(s) 523
BsaHI GRCGYC 1 cut(s) 680
BsaI GGTCTC 1 cut(s) 765
BsaJI CCNNGG 1 cut(s) 742
BsaWI WCCGGW 1 cut(s) 570
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 381, 943
Bse118I RCCGGY 2 cut(s) 887, 1199
Bse1I ACTGG 2 cut(s) 816, 987
BseDI CCNNGG 1 cut(s) 742
BseGI GGATG 2 cut(s) 22, 114
BseLI CCNNNNNNNGG 3 cut(s) 132, 381, 943
BseNI ACTGG 2 cut(s) 816, 987
BseRI GAGGAG 2 cut(s) 884, 887
BseXI GCAGC 3 cut(s) 41, 285, 921
BsgI GTGCAG 1 cut(s) 1226
Bsh1236I CGCG 1 cut(s) 281
Bsh1285I CGRYCG 1 cut(s) 579
BshFI GGCC 3 cut(s) 241, 891, 935
BshNI GGYRCC 1 cut(s) 1123
BsiEI CGRYCG 1 cut(s) 579
BsiHKAI GWGCWC 1 cut(s) 699
BsiSI CCGG 4 cut(s) 571, 742, 888, 1200
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 3 cut(s) 132, 381, 943
BsmAI GTCTC 2 cut(s) 149, 765
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 3 cut(s) 241, 891, 935
Bso31I GGTCTC 1 cut(s) 765
Bsp1286I GDGCHC 2 cut(s) 330, 699
Bsp143I GATC 6 cut(s) 84, 92, 118, 723, 771, 1036
Bsp68I TCGCGA 1 cut(s) 281
BspACI CCGC 3 cut(s) 186, 822, 907
BspANI GGCC 3 cut(s) 241, 891, 935
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 4 cut(s) 329, 782, 1000, 1125
BspMI ACCTGC 2 cut(s) 108, 227
BspPI GGATC 3 cut(s) 87, 126, 1031
BspT107I GGYRCC 1 cut(s) 1123
BspTI CTTAAG 1 cut(s) 631
BspTNI GGTCTC 1 cut(s) 765
BsrFI RCCGGY 2 cut(s) 887, 1199
BsrI ACTGG 2 cut(s) 816, 987
BssAI RCCGGY 2 cut(s) 887, 1199
BssECI CCNNGG 1 cut(s) 742
BssMI GATC 6 cut(s) 84, 92, 118, 723, 771, 1036
BssNI GRCGYC 1 cut(s) 680
BssSI CACGAG 1 cut(s) 590
Bst2BI CACGAG 1 cut(s) 590
Bst4CI ACNGT 8 cut(s) 251, 286, 376, 535, 555, 580, 655, 1210
BstACI GRCGYC 1 cut(s) 680
BstAFI CTTAAG 1 cut(s) 631
BstBAI YACGTR 1 cut(s) 523
BstC8I GCNNGC 4 cut(s) 211, 541, 949, 1201
BstDEI CTNAG 2 cut(s) 701, 1311
BstEII GGTNACC 2 cut(s) 883, 1174
BstF5I GGATG 2 cut(s) 22, 114
BstFNI CGCG 1 cut(s) 281
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 3 cut(s) 54, 215, 914
BstKTI GATC 6 cut(s) 87, 95, 121, 726, 774, 1039
BstMAI GTCTC 2 cut(s) 149, 765
BstMBI GATC 6 cut(s) 84, 92, 118, 723, 771, 1036
BstMCI CGRYCG 1 cut(s) 579
BstMWI GCNNNNNNNGC 3 cut(s) 219, 953, 1028
BstNSI RCATGY 6 cut(s) 144, 160, 543, 951, 971, 1249
BstPI GGTNACC 2 cut(s) 883, 1174
BstSCI CCNGG 1 cut(s) 741
BstSFI CTRYAG 1 cut(s) 534
BstSNI TACGTA 1 cut(s) 523
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 3 cut(s) 41, 285, 921
BstV2I GAAGAC 1 cut(s) 1298
BstX2I RGATCY 2 cut(s) 84, 92
BstXI CCANNNNNNTGG 1 cut(s) 880
BstYI RGATCY 2 cut(s) 84, 92
BsuRI GGCC 3 cut(s) 241, 891, 935
BtgZI GCGATG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 676
BtsCI GGATG 2 cut(s) 22, 114
BtsI GCAGTG 2 cut(s) 948, 1238
BtsIMutI CAGTG 7 cut(s) 256, 531, 660, 823, 948, 980, 1238
BtuMI TCGCGA 1 cut(s) 281
BveI ACCTGC 2 cut(s) 108, 227
Cac8I GCNNGC 4 cut(s) 211, 541, 949, 1201
CfoI GCGC 3 cut(s) 54, 215, 914
Cfr10I RCCGGY 2 cut(s) 887, 1199
Cfr13I GGNCC 2 cut(s) 240, 934
CseI GACGC 2 cut(s) 265, 669
Csp6I GTAC 1 cut(s) 478
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 2 cut(s) 701, 1311
DpnI GATC 6 cut(s) 86, 94, 120, 725, 773, 1038
DpnII GATC 6 cut(s) 84, 92, 118, 723, 771, 1036
DriI GACNNNNNGTC 1 cut(s) 679
Eam1105I GACNNNNNGTC 1 cut(s) 679
Eco105I TACGTA 1 cut(s) 523
Eco24I GRGCYC 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 765
Eco47III AGCGCT 1 cut(s) 53
Eco91I GGTNACC 2 cut(s) 883, 1174
EcoO109I RGGNCCY 1 cut(s) 240
EcoO65I GGTNACC 2 cut(s) 883, 1174
EcoT22I ATGCAT 1 cut(s) 545
EcoT38I GRGCYC 1 cut(s) 330
FaqI GGGAC 1 cut(s) 167
FauI CCCGC 1 cut(s) 193
FbaI TGATCA 1 cut(s) 771
Fnu4HI GCNGC 4 cut(s) 55, 274, 822, 910
FokI GGATG 2 cut(s) 29, 121
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 4 cut(s) 55, 274, 822, 910
FspBI CTAG 2 cut(s) 548, 953
GlaI GCGC 3 cut(s) 53, 214, 913
GluI GCNGC 4 cut(s) 55, 274, 822, 910
GsuI CTGGAG 1 cut(s) 1004
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 3 cut(s) 241, 891, 935
HapII CCGG 4 cut(s) 571, 742, 888, 1200
HgaI GACGC 2 cut(s) 265, 669
HhaI GCGC 3 cut(s) 54, 215, 914
Hin1I GRCGYC 1 cut(s) 680
Hin6I GCGC 3 cut(s) 52, 213, 912
HinP1I GCGC 3 cut(s) 52, 213, 912
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 8 cut(s) 174, 489, 668, 705, 738, 986, 1010, 1067
HpaII CCGG 4 cut(s) 571, 742, 888, 1200
HphI GGTGA 1 cut(s) 1186
Hpy166II GTNNAC 4 cut(s) 480, 520, 1213, 1221
Hpy188I TCNGA 6 cut(s) 332, 673, 694, 710, 848, 1072
Hpy188III TCNNGA 6 cut(s) 280, 379, 486, 548, 641, 805
Hpy8I GTNNAC 4 cut(s) 480, 520, 1213, 1221
Hpy99I CGWCG 5 cut(s) 227, 281, 286, 677, 1064
HpyAV CCTTC 2 cut(s) 327, 705
HpyCH4III ACNGT 8 cut(s) 251, 286, 376, 535, 555, 580, 655, 1210
HpyCH4IV ACGT 4 cut(s) 522, 675, 1162, 1223
HpyCH4V TGCA 4 cut(s) 82, 156, 543, 1243
HpyF10VI GCNNNNNNNGC 3 cut(s) 219, 953, 1028
HpyF3I CTNAG 2 cut(s) 701, 1311
HpySE526I ACGT 4 cut(s) 522, 675, 1162, 1223
Hsp92I GRCGYC 1 cut(s) 680
HspAI GCGC 3 cut(s) 52, 213, 912
KroI GCCGGC 1 cut(s) 1199
KroNI GCCGGC 1 cut(s) 1201
Ksp22I TGATCA 1 cut(s) 771
Kzo9I GATC 6 cut(s) 84, 92, 118, 723, 771, 1036
LmnI GCTCC 3 cut(s) 333, 780, 875
Lsp1109I GCAGC 3 cut(s) 41, 285, 921
LweI GCATC 1 cut(s) 7
MaeI CTAG 2 cut(s) 548, 953
MaeII ACGT 4 cut(s) 522, 675, 1162, 1223
MaeIII GTNAC 2 cut(s) 883, 1174
MalI GATC 6 cut(s) 86, 94, 120, 725, 773, 1038
MboI GATC 6 cut(s) 84, 92, 118, 723, 771, 1036
MboII GAAGA 3 cut(s) 1076, 1106, 1303
MflI RGATCY 2 cut(s) 84, 92
MhlI GDGCHC 2 cut(s) 330, 699
MlyI GAGTC 1 cut(s) 995
MmeI TCCRAC 4 cut(s) 696, 987, 1040, 1206
MnlI CCTC 8 cut(s) 231, 311, 375, 599, 632, 902, 905, 908
Mph1103I ATGCAT 1 cut(s) 545
MroNI GCCGGC 1 cut(s) 1199
MseI TTAA 7 cut(s) 26, 401, 632, 719, 1053, 1271, 1299
MslI CAYNNNNRTG 4 cut(s) 69, 295, 972, 1118
MspA1I CMGCKG 1 cut(s) 824
MspCI CTTAAG 1 cut(s) 631
MspI CCGG 4 cut(s) 571, 742, 888, 1200
MspR9I CCNGG 1 cut(s) 743
Mva1269I GAATGC 1 cut(s) 543
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 219, 953, 1028
NaeI GCCGGC 1 cut(s) 1201
NciI CCSGG 1 cut(s) 743
NdeII GATC 6 cut(s) 84, 92, 118, 723, 771, 1036
NgoMIV GCCGGC 1 cut(s) 1199
NlaIV GGNNCC 4 cut(s) 329, 782, 1000, 1125
NmuCI GTSAC 1 cut(s) 1174
NruI TCGCGA 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 545
NspI RCATGY 6 cut(s) 144, 160, 543, 951, 971, 1249
PaeI GCATGC 2 cut(s) 543, 951
PaqCI CACCTGC 1 cut(s) 227
PciI ACATGT 1 cut(s) 1245
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PctI GAATGC 1 cut(s) 543
PdiI GCCGGC 1 cut(s) 1201
PfeI GAWTC 7 cut(s) 174, 489, 668, 705, 738, 1010, 1067
PflFI GACNNNGTC 3 cut(s) 251, 286, 811
PflMI CCANNNNNTGG 2 cut(s) 132, 943
PkrI GCNGC 4 cut(s) 56, 275, 823, 911
PleI GAGTC 1 cut(s) 994
PpsI GAGTC 1 cut(s) 994
Ppu21I YACGTR 1 cut(s) 523
PscI ACATGT 1 cut(s) 1245
PshBI ATTAAT 2 cut(s) 1271, 1299
PspEI GGTNACC 2 cut(s) 883, 1174
PspN4I GGNNCC 4 cut(s) 329, 782, 1000, 1125
PspPI GGNCC 2 cut(s) 240, 934
PsuI RGATCY 2 cut(s) 84, 92
PsyI GACNNNGTC 3 cut(s) 251, 286, 811
RruI TCGCGA 1 cut(s) 281
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
RseI CAYNNNNRTG 4 cut(s) 69, 295, 972, 1118
SaqAI TTAA 7 cut(s) 26, 401, 632, 719, 1053, 1271, 1299
SatI GCNGC 4 cut(s) 55, 274, 822, 910
Sau3AI GATC 6 cut(s) 84, 92, 118, 723, 771, 1036
Sau96I GGNCC 2 cut(s) 240, 934
SchI GAGTC 1 cut(s) 995
ScrFI CCNGG 1 cut(s) 743
SduI GDGCHC 2 cut(s) 330, 699
SfaNI GCATC 1 cut(s) 7
SfcI CTRYAG 1 cut(s) 534
SmiMI CAYNNNNRTG 4 cut(s) 69, 295, 972, 1118
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
SnaBI TACGTA 1 cut(s) 523
SphI GCATGC 2 cut(s) 543, 951
SsiI CCGC 3 cut(s) 186, 822, 907
SspMI CTAG 2 cut(s) 548, 953
StyD4I CCNGG 1 cut(s) 741
TaaI ACNGT 8 cut(s) 251, 286, 376, 535, 555, 580, 655, 1210
TaiI ACGT 4 cut(s) 525, 678, 1165, 1226
TaqI TCGA 2 cut(s) 666, 828
TauI GCSGC 1 cut(s) 824
TfiI GAWTC 7 cut(s) 174, 489, 668, 705, 738, 1010, 1067
Tru1I TTAA 7 cut(s) 26, 401, 632, 719, 1053, 1271, 1299
Tru9I TTAA 7 cut(s) 26, 401, 632, 719, 1053, 1271, 1299
TscAI CASTG 7 cut(s) 256, 538, 660, 823, 948, 987, 1245
TseFI GTSAC 1 cut(s) 1174
TseI GCWGC 3 cut(s) 54, 273, 909
Tsp45I GTSAC 1 cut(s) 1174
TspDTI ATGAA 7 cut(s) 90, 151, 549, 739, 1107, 1125, 1257
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 7 cut(s) 256, 538, 660, 823, 948, 987, 1245
Tth111I GACNNNGTC 3 cut(s) 251, 286, 811
Van91I CCANNNNNTGG 2 cut(s) 132, 943
Vha464I CTTAAG 1 cut(s) 631
VspI ATTAAT 2 cut(s) 1271, 1299
XapI RAATTY 3 cut(s) 417, 1262, 1305
XbaI TCTAGA 1 cut(s) 547
XceI RCATGY 6 cut(s) 144, 160, 543, 951, 971, 1249
XspI CTAG 2 cut(s) 548, 953
Zsp2I ATGCAT 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.