AT1G54860

gpi-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005399
Physical Location & Seq
Reverse (-)
20457257 .. 20458631
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54860.1

Sequence Viewer

Length: 603 bp
ATGGTCTTGATTACTAAGATGTCTCTCTCTTTCTACATCATTCATCTTCTCATTTTCTCCTTGATTTCCACTTGTGTTGTCTCCAACCAGGCCGAGGATAATCTTCTTCAAGGCCTAAACAGCTACCGAACTGCTCAAAGAGTTCCTCCATTTGCCAAGAATGAGAAGGCTGATTGTGTGGCTGATGAGATCGCCGACAAGCTCGAAGATCAGCCATGCACAAACCACACCACAGCGAGCACGGTTACTCCTGGCTCGGTACCTCCACGGCTGACGAACTACCAGGACATTCTCTCTGAATGCAAAATCGACCCAAACACTACCCGTGACGGATTGATCTTACCTGTCTGTATCCCTAACCGGATCCCGACTTTGGCTTTAACTAATTACACCCAAACTGGTTATGCTCGGTATCTTAATGATTCGAGGTATGTCGGGGCTGGTGTTGGGTCGGAGAAAGAGTGGATGGTGGTTGTATTGACGACAAGTACTCCAGGTGGAAGCTTTACAGCTGGTGTTGCTGCTGGCAAGGCGACGTCTGTGAGAGTAATGGCTGGTTTAGGGCTAATGGGGTTGTTGTTTAGTTGCCTTGTGCTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

21.57

Weight (kDa)

6.09

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 32 - 160 4.9e-52 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 539
Acc65I GGTACC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 259
AclWI GGATC 2 cut(s) 358, 371
AcyI GRCGYC 1 cut(s) 536
AfaI GTAC 2 cut(s) 261, 490
AfiI CCNNNNNNNGG 2 cut(s) 94, 373
AgsI TTSAA 1 cut(s) 110
AjnI CCWGG 4 cut(s) 87, 250, 282, 493
AluBI AGCT 4 cut(s) 123, 202, 504, 512
AluI AGCT 4 cut(s) 123, 202, 504, 512
Alw21I GWGCWC 2 cut(s) 242, 597
Alw26I GTCTC 2 cut(s) 27, 85
AlwI GGATC 2 cut(s) 358, 371
AoxI GGCC 2 cut(s) 90, 112
ApeKI GCWGC 1 cut(s) 521
Asp718I GGTACC 1 cut(s) 259
BamHI GGATCC 1 cut(s) 363
BanI GGYRCC 1 cut(s) 259
Bbv12I GWGCWC 2 cut(s) 242, 597
BbvI GCAGC 1 cut(s) 508
BccI CCATC 1 cut(s) 460
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 4 cut(s) 89, 252, 284, 495
BciVI GTATCC 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 27, 85
BfuI GTATCC 1 cut(s) 362
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 4 cut(s) 89, 252, 284, 495
BmiI GGNNCC 2 cut(s) 261, 365
BmrFI CCNGG 4 cut(s) 89, 252, 284, 495
BpmI CTGGAG 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 536
BsaJI CCNNGG 2 cut(s) 93, 266
BsaWI WCCGGW 1 cut(s) 360
BsaXI ACNNNNNCTCC 6 cut(s) 232, 262, 446, 475, 476, 505
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 373
Bse1I ACTGG 1 cut(s) 403
BseBI CCWGG 4 cut(s) 89, 252, 284, 495
BseDI CCNNGG 2 cut(s) 93, 266
BseGI GGATG 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 94, 373
BseNI ACTGG 1 cut(s) 403
BseXI GCAGC 1 cut(s) 508
BshFI GGCC 2 cut(s) 92, 114
BshNI GGYRCC 1 cut(s) 259
BsiHKAI GWGCWC 2 cut(s) 242, 597
BsiSI CCGG 1 cut(s) 361
BslI CCNNNNNNNGG 2 cut(s) 94, 373
BsmAI GTCTC 2 cut(s) 27, 85
BsmI GAATGC 1 cut(s) 305
BsnI GGCC 2 cut(s) 92, 114
Bsp1286I GDGCHC 2 cut(s) 242, 597
Bsp143I GATC 4 cut(s) 189, 208, 336, 363
BspANI GGCC 2 cut(s) 92, 114
BspLI GGNNCC 2 cut(s) 261, 365
BspPI GGATC 2 cut(s) 358, 371
BspT107I GGYRCC 1 cut(s) 259
BsrI ACTGG 1 cut(s) 403
BssECI CCNNGG 2 cut(s) 93, 266
BssMI GATC 4 cut(s) 189, 208, 336, 363
BssNI GRCGYC 1 cut(s) 536
Bst2UI CCWGG 4 cut(s) 89, 252, 284, 495
Bst4CI ACNGT 1 cut(s) 244
BstACI GRCGYC 1 cut(s) 536
BstC8I GCNNGC 2 cut(s) 238, 526
BstDEI CTNAG 1 cut(s) 15
BstDSI CCRYGG 1 cut(s) 266
BstF5I GGATG 1 cut(s) 471
BstKTI GATC 4 cut(s) 192, 211, 339, 366
BstMAI GTCTC 2 cut(s) 27, 85
BstMBI GATC 4 cut(s) 189, 208, 336, 363
BstMWI GCNNNNNNNGC 3 cut(s) 120, 518, 530
BstNI CCWGG 4 cut(s) 89, 252, 284, 495
BstSCI CCNGG 4 cut(s) 87, 250, 282, 493
BstV1I GCAGC 1 cut(s) 508
BstX2I RGATCY 1 cut(s) 363
BstYI RGATCY 1 cut(s) 363
BsuI GTATCC 1 cut(s) 362
BsuRI GGCC 2 cut(s) 92, 114
BtgI CCRYGG 1 cut(s) 266
BtsCI GGATG 1 cut(s) 471
Cac8I GCNNGC 2 cut(s) 238, 526
Csp6I GTAC 2 cut(s) 260, 489
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 1 cut(s) 216
CviQI GTAC 2 cut(s) 260, 489
DdeI CTNAG 1 cut(s) 15
DpnI GATC 4 cut(s) 191, 210, 338, 365
DpnII GATC 4 cut(s) 189, 208, 336, 363
Eco147I AGGCCT 1 cut(s) 114
EcoRII CCWGG 4 cut(s) 87, 250, 282, 493
FaeI CATG 1 cut(s) 219
FaiI YATR 3 cut(s) 217, 405, 432
FatI CATG 1 cut(s) 215
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 1 cut(s) 478
Fsp4HI GCNGC 1 cut(s) 522
GluI GCNGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 2 cut(s) 92, 114
HapII CCGG 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 536
Hin1II CATG 1 cut(s) 219
HindIII AAGCTT 1 cut(s) 502
HinfI GANTC 1 cut(s) 422
HpaII CCGG 1 cut(s) 361
Hpy188I TCNGA 3 cut(s) 298, 454, 602
Hpy188III TCNNGA 2 cut(s) 7, 367
Hpy99I CGWCG 1 cut(s) 538
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 2 cut(s) 219, 303
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 518, 530
HpyF3I CTNAG 1 cut(s) 15
HpySE526I ACGT 1 cut(s) 536
Hsp92I GRCGYC 1 cut(s) 536
Hsp92II CATG 1 cut(s) 219
KpnI GGTACC 1 cut(s) 263
Kzo9I GATC 4 cut(s) 189, 208, 336, 363
Lsp1109I GCAGC 1 cut(s) 508
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 2 cut(s) 244, 326
MalI GATC 4 cut(s) 191, 210, 338, 365
MboI GATC 4 cut(s) 189, 208, 336, 363
MboII GAAGA 5 cut(s) 38, 95, 98, 218, 589
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 2 cut(s) 242, 597
MluCI AATT 1 cut(s) 385
MmeI TCCRAC 2 cut(s) 108, 432
MnlI CCTC 4 cut(s) 88, 156, 273, 420
MseI TTAA 2 cut(s) 380, 417
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 4 cut(s) 89, 252, 284, 495
Mva1269I GAATGC 1 cut(s) 305
MvaI CCWGG 4 cut(s) 89, 252, 284, 495
MwoI GCNNNNNNNGC 3 cut(s) 120, 518, 530
NdeII GATC 4 cut(s) 189, 208, 336, 363
NlaIII CATG 1 cut(s) 219
NlaIV GGNNCC 2 cut(s) 261, 365
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 326
PceI AGGCCT 1 cut(s) 114
PctI GAATGC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 422
PkrI GCNGC 1 cut(s) 523
Psp6I CCWGG 4 cut(s) 87, 250, 282, 493
PspGI CCWGG 4 cut(s) 87, 250, 282, 493
PspN4I GGNNCC 2 cut(s) 261, 365
PsuI RGATCY 1 cut(s) 363
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 2 cut(s) 261, 490
RsaNI GTAC 2 cut(s) 260, 489
SaqAI TTAA 2 cut(s) 380, 417
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 4 cut(s) 189, 208, 336, 363
ScaI AGTACT 1 cut(s) 490
ScrFI CCNGG 4 cut(s) 89, 252, 284, 495
SduI GDGCHC 2 cut(s) 242, 597
SetI ASST 9 cut(s) 125, 204, 265, 346, 431, 499, 506, 514, 539
Sse9I AATT 1 cut(s) 385
SseBI AGGCCT 1 cut(s) 114
StuI AGGCCT 1 cut(s) 114
StyD4I CCNGG 4 cut(s) 87, 250, 282, 493
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 1 cut(s) 539
TaqI TCGA 3 cut(s) 204, 309, 425
TasI AATT 1 cut(s) 385
TatI WGTACW 1 cut(s) 488
TfiI GAWTC 1 cut(s) 422
Tru1I TTAA 2 cut(s) 380, 417
Tru9I TTAA 2 cut(s) 380, 417
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 1 cut(s) 32
TspGWI ACGGA 1 cut(s) 345
ZraI GACGTC 1 cut(s) 537
ZrmI AGTACT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.