RchiOBHm_Chr3g0454541

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005399
Physical Location & Seq
Forward (+)
4635121 .. 4635642
522 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42155

Sequence Viewer

Length: 522 bp
ATGGCAGAAGCCAATGAAGATGATATTCTCCAAGGCATCAACAGCTACAGACGATCACTGAGCTTACCGAACCTCATCAAGCACGACAAAGCGGATTGCCTAGCAGAAGAAATTGCTGATGATATACAGGACCAACCATGCAGCAGCCGTACCAATGGCGCCAACATCTCACCAAAATCATCAACTGAACTCCCCAGTCTTCCCAGTAACTTGATCAAGTGCAAAATAGATGTGAACAGCACAGTGGATGGGGTCATAATGCCAGTTTGTGTGCCCAAAGCAGTCTCAACTCTTGTGCTTACCAATTACACTAGCTCCCCGCGCTACGCAAAATTTCTGAATGATTCGAGGTTCACTGGGGTTGGAATTGGTACCGAGGACGATTGGACTGTGGTGGTTTTGGCAACAAGTACCCGAACAGGAAGCTTTGCCAATGCAGTAAACTCTTTGGTTTCGACGGTTGGCGCTGTCCATTACTTGGTCTCCTTAATGTTGGGGTTGTGGCTTATTTTGGTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

18.56

Weight (kDa)

4.89

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 6 - 134 1e-51 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 2 cut(s) 158, 371
AccB7I CCANNNNNTGG 1 cut(s) 478
AccII CGCG 1 cut(s) 322
AciI CCGC 2 cut(s) 92, 320
AcsI RAATTY 1 cut(s) 332
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 3 cut(s) 151, 373, 412
AfiI CCNNNNNNNGG 1 cut(s) 478
AluBI AGCT 5 cut(s) 45, 63, 315, 426, 519
AluI AGCT 5 cut(s) 45, 63, 315, 426, 519
Alw26I GTCTC 2 cut(s) 289, 487
ApeKI GCWGC 2 cut(s) 141, 144
ApoI RAATTY 1 cut(s) 332
Asp718I GGTACC 1 cut(s) 371
AspLEI GCGC 3 cut(s) 161, 324, 467
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 162
AvaII GGWCC 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 276
BanI GGYRCC 2 cut(s) 158, 371
BbsI GAAGAC 1 cut(s) 191
BbvI GCAGC 2 cut(s) 153, 156
BccI CCATC 1 cut(s) 242
BceAI ACGGC 1 cut(s) 132
BclI TGATCA 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 289, 487
BfaI CTAG 2 cut(s) 101, 312
BfmI CTRYAG 1 cut(s) 46
BfoI RGCGCY 2 cut(s) 162, 468
BisI GCNGC 2 cut(s) 142, 145
BlsI GCNGC 2 cut(s) 143, 146
Bme18I GGWCC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 2 cut(s) 160, 373
BmrI ACTGGG 3 cut(s) 189, 198, 366
BmsI GCATC 1 cut(s) 45
BmuI ACTGGG 3 cut(s) 189, 198, 366
BpiI GAAGAC 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 2 cut(s) 31, 375
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse1I ACTGG 4 cut(s) 195, 204, 263, 361
BseDI CCNNGG 2 cut(s) 31, 375
BseGI GGATG 1 cut(s) 253
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 4 cut(s) 195, 204, 263, 361
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 153, 156
Bsh1236I CGCG 1 cut(s) 322
BshNI GGYRCC 2 cut(s) 158, 371
BslI CCNNNNNNNGG 1 cut(s) 478
BsmAI GTCTC 2 cut(s) 289, 487
Bso31I GGTCTC 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 2 cut(s) 53, 213
BspACI CCGC 2 cut(s) 92, 320
BspCNI CTCAG 1 cut(s) 51
BspFNI CGCG 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 160, 373
BspT107I GGYRCC 2 cut(s) 158, 371
BspTNI GGTCTC 1 cut(s) 487
BsrI ACTGG 4 cut(s) 195, 204, 263, 361
BssECI CCNNGG 2 cut(s) 31, 375
BssMI GATC 2 cut(s) 53, 213
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 31
Bst4CI ACNGT 3 cut(s) 244, 391, 460
BstACI GRCGYC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 59
BstF5I GGATG 1 cut(s) 253
BstFNI CGCG 1 cut(s) 322
BstH2I RGCGCY 2 cut(s) 162, 468
BstHHI GCGC 3 cut(s) 161, 324, 467
BstKTI GATC 2 cut(s) 56, 216
BstMAI GTCTC 2 cut(s) 289, 487
BstMBI GATC 2 cut(s) 53, 213
BstMWI GCNNNNNNNGC 2 cut(s) 42, 321
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 2 cut(s) 153, 156
BstV2I GAAGAC 1 cut(s) 191
BtsCI GGATG 1 cut(s) 253
BtsIMutI CAGTG 3 cut(s) 56, 249, 354
CfoI GCGC 3 cut(s) 161, 324, 467
Cfr13I GGNCC 1 cut(s) 130
Csp6I GTAC 3 cut(s) 150, 372, 411
CviAII CATG 1 cut(s) 138
CviJI RGCY 8 cut(s) 11, 45, 63, 147, 315, 426, 505, 519
CviKI_1 RGCY 8 cut(s) 11, 45, 63, 147, 315, 426, 505, 519
CviQI GTAC 3 cut(s) 150, 372, 411
DdeI CTNAG 1 cut(s) 59
DinI GGCGCC 1 cut(s) 160
DpnI GATC 2 cut(s) 55, 215
DpnII GATC 2 cut(s) 53, 213
Eco130I CCWWGG 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 31
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 1 cut(s) 141
FaiI YATR 3 cut(s) 125, 139, 257
FatI CATG 1 cut(s) 137
FauI CCCGC 1 cut(s) 327
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 2 cut(s) 142, 145
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 2 cut(s) 142, 145
FspBI CTAG 2 cut(s) 101, 312
GlaI GCGC 3 cut(s) 160, 323, 466
GluI GCNGC 2 cut(s) 142, 145
HaeII RGCGCY 2 cut(s) 162, 468
HhaI GCGC 3 cut(s) 161, 324, 467
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 1 cut(s) 141
Hin6I GCGC 3 cut(s) 159, 322, 465
HinP1I GCGC 3 cut(s) 159, 322, 465
HindIII AAGCTT 1 cut(s) 424
HinfI GANTC 1 cut(s) 344
HphI GGTGA 1 cut(s) 162
Hpy166II GTNNAC 3 cut(s) 235, 354, 442
Hpy188I TCNGA 1 cut(s) 339
Hpy8I GTNNAC 3 cut(s) 235, 354, 442
Hpy99I CGWCG 1 cut(s) 460
HpyCH4III ACNGT 3 cut(s) 244, 391, 460
HpyCH4V TGCA 3 cut(s) 141, 222, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 321
HpyF3I CTNAG 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 1 cut(s) 141
HspAI GCGC 3 cut(s) 159, 322, 465
KasI GGCGCC 1 cut(s) 158
KpnI GGTACC 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 2 cut(s) 53, 213
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 6 cut(s) 113, 208, 217, 276, 342, 405
Lsp1109I GCAGC 2 cut(s) 153, 156
LweI GCATC 1 cut(s) 45
MaeI CTAG 2 cut(s) 101, 312
MaeIII GTNAC 1 cut(s) 206
MalI GATC 2 cut(s) 55, 215
MboI GATC 2 cut(s) 53, 213
MboII GAAGA 3 cut(s) 29, 119, 191
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 4 cut(s) 111, 304, 332, 366
Mly113I GGCGCC 1 cut(s) 159
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 3 cut(s) 83, 342, 370
MseI TTAA 1 cut(s) 488
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 42, 321
NarI GGCGCC 1 cut(s) 159
NdeII GATC 2 cut(s) 53, 213
NlaIII CATG 1 cut(s) 141
NlaIV GGNNCC 2 cut(s) 160, 373
PfeI GAWTC 1 cut(s) 344
PflMI CCANNNNNTGG 1 cut(s) 478
PkrI GCNGC 2 cut(s) 143, 146
PluTI GGCGCC 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 160, 373
PspPI GGNCC 1 cut(s) 130
RsaI GTAC 3 cut(s) 151, 373, 412
RsaNI GTAC 3 cut(s) 150, 372, 411
SaqAI TTAA 1 cut(s) 488
SatI GCNGC 2 cut(s) 142, 145
Sau3AI GATC 2 cut(s) 53, 213
Sau96I GGNCC 1 cut(s) 130
SduI GDGCHC 1 cut(s) 276
SetI ASST 7 cut(s) 47, 65, 75, 317, 353, 428, 521
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 1 cut(s) 46
SfoI GGCGCC 1 cut(s) 160
SinI GGWCC 1 cut(s) 130
Sse9I AATT 4 cut(s) 111, 304, 332, 366
SsiI CCGC 2 cut(s) 92, 320
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 2 cut(s) 101, 312
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 3 cut(s) 244, 391, 460
TaqI TCGA 2 cut(s) 347, 455
TasI AATT 4 cut(s) 111, 304, 332, 366
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TscAI CASTG 3 cut(s) 63, 249, 361
TseI GCWGC 2 cut(s) 141, 144
TspDTI ATGAA 1 cut(s) 30
TspRI CASTG 3 cut(s) 63, 249, 361
Van91I CCANNNNNTGG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 130
XapI RAATTY 1 cut(s) 332
XspI CTAG 2 cut(s) 101, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.