pycom04g18270

gpi-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005399
Physical Location & Seq
Reverse (-)
20367867 .. 20369732
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18270.2

Sequence Viewer

Length: 1182 bp
ATGGCATTTGATATGATTTTAGAATTTCAGTGGCTAATCCATTCTCAAAACTTATACTTTACCTATTTGCCTGCAGATAAGGATGAGGACAACCTTCTCCAAGGCATTAACAGTTACAGAACCTCGCTAAACCTGCCGGCCCTCGTCAAGAACGGTAATGCAGGCTGCCTAGCGGATCAAATTGCGGATGACATGGAAGACCAGCCTTGCAACAGTCCCACCAACGGACACTTGAGCAAGTGCAAAATCGATGCCAACAGCACATCAGCCGGAGTTATTCTCCCAATTTGTGTGCCTGATTTGGTCCCAACTGTGGTGCTTACCAATTATACTCACCGACCGCAGTTCGTAAAGTATCTGAACGATTCGAAGTTCACTGGAGTTGGGCTTGGATCCGAGGATGATTGGATGGTGGTGGTTTTGGCCACAAATAACCCAGCTGGAAGCTTTGTTAATGCAGCAAGCTCTTTAGTTTCAAGTTCAACTGTTGGTTTTGGTCGTTTCTTGGTCACCCTTTTGCTAGGCAATCTTTTTATTCCTCCCCAACTGGTGTATATTTATGCACCCTATTCACAAGGCCGATGTCCTAGCACTAAAACAATGGCCTTCTCCAAACTAAGTTTCTTTCTCTTTTATGTTATTGGCTTACTATTTATTTCTCATCCAATATCTTCAAACGATGAAGAAGACAAGCTTTTCCAAGGTTTCAACAACTTCAGGCAATCCCAAAAGCTTCCGGAGTTCAAGAAGAACGACAATGCAGCTTGTCTAGCCGATAAACTTGCCGACAAGCTAGAATACCAACAGTGTTCTAGCTCACAGAAGTACAGCATCGAGCCAGGCAATGGACCCAAATTCCCGAAATTCGACAAGCTCAGGAGAAAGTGCCATATCGAAACCAACACGACAACAGCCGGCATCATTTGGCCCGTCTGCGTGCCGGATTTGGACGAGGATCTTGTGCTCAACAACTACACTCGCTCCCATTACGCAAAATATCTGAAGGATTCTAAGTATGCAGGTGTTGGGATTGGTTCTGAGGATGAATGGGTGATTGTTGTGTTGACCACTGACGCACAAAGTGGAAGCTTTGCGAGCGGCGCAGCTTCTTTGGGTGCCATTGGCATGATTCATTACATGGCGGCTGTTTTGGTGGGGTTGTTCTTGGTTTTGCTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

43.24

Weight (kDa)

5.39

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1010
Acc36I ACCTGC 2 cut(s) 141, 1010
AccB1I GGYRCC 1 cut(s) 1115
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 1098
AccIII TCCGGA 1 cut(s) 736
AciI CCGC 5 cut(s) 173, 185, 341, 1098, 1142
AclWI GGATC 4 cut(s) 183, 387, 400, 963
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 3 cut(s) 23, 854, 863
AcuI CTGAAG 2 cut(s) 700, 1022
AdeI CACNNNGTG 1 cut(s) 1082
AfaI GTAC 1 cut(s) 827
AfiI CCNNNNNNNGG 3 cut(s) 224, 313, 845
AgsI TTSAA 5 cut(s) 477, 483, 675, 709, 745
AjnI CCWGG 1 cut(s) 838
AjuI GAANNNNNNNTTGG 2 cut(s) 605, 637
Alw21I GWGCWC 1 cut(s) 966
AlwI GGATC 4 cut(s) 183, 387, 400, 963
Aor13HI TCCGGA 1 cut(s) 736
AoxI GGCC 5 cut(s) 138, 423, 577, 603, 926
ApeKI GCWGC 4 cut(s) 165, 458, 761, 1103
ApoI RAATTY 3 cut(s) 23, 854, 863
AspLEI GCGC 1 cut(s) 1103
AspS9I GGNCC 4 cut(s) 139, 304, 848, 927
AsuHPI GGTGA 3 cut(s) 326, 502, 1063
AsuII TTCGAA 1 cut(s) 368
AvaII GGWCC 2 cut(s) 304, 848
BalI TGGCCA 1 cut(s) 425
BamHI GGATCC 1 cut(s) 392
BanI GGYRCC 1 cut(s) 1115
BbsI GAAGAC 2 cut(s) 204, 693
Bbv12I GWGCWC 1 cut(s) 966
BbvI GCAGC 4 cut(s) 152, 470, 773, 1115
BccI CCATC 1 cut(s) 403
BcgI CGANNNNNNTGC 2 cut(s) 764, 798
BciT130I CCWGG 1 cut(s) 840
BfaI CTAG 6 cut(s) 170, 521, 588, 770, 794, 813
BfmI CTRYAG 1 cut(s) 72
BfuAI ACCTGC 2 cut(s) 141, 1010
BisI GCNGC 6 cut(s) 166, 459, 762, 1099, 1104, 1143
BlsI GCNGC 6 cut(s) 167, 460, 763, 1100, 1105, 1144
Bme1390I CCNGG 1 cut(s) 840
Bme18I GGWCC 2 cut(s) 304, 848
BmgT120I GGNCC 4 cut(s) 139, 304, 848, 927
BmiI GGNNCC 4 cut(s) 306, 394, 850, 1117
BmrFI CCNGG 1 cut(s) 840
BmsI GCATC 3 cut(s) 241, 840, 927
BpiI GAAGAC 2 cut(s) 204, 693
BplI GAGNNNNNCTC 2 cut(s) 264, 296
BpmI CTGGAG 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 875
Bpu14I TTCGAA 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 253
Bsa29I ATCGAT 1 cut(s) 249
BsaJI CCNNGG 3 cut(s) 100, 396, 700
BsaWI WCCGGW 1 cut(s) 736
BsaXI ACNNNNNCTCC 2 cut(s) 965, 995
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 313, 845
Bse118I RCCGGY 2 cut(s) 136, 914
Bse1I ACTGG 2 cut(s) 382, 552
Bse3DI GCAATG 1 cut(s) 850
BseAI TCCGGA 1 cut(s) 736
BseBI CCWGG 1 cut(s) 840
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 3 cut(s) 100, 396, 700
BseGI GGATG 6 cut(s) 88, 193, 406, 414, 661, 1048
BseLI CCNNNNNNNGG 3 cut(s) 224, 313, 845
BseMI GCAATG 1 cut(s) 850
BseMII CTCAG 2 cut(s) 889, 1029
BseNI ACTGG 2 cut(s) 382, 552
BseXI GCAGC 4 cut(s) 152, 470, 773, 1115
BseYI CCCAGC 1 cut(s) 436
Bsh1285I CGRYCG 1 cut(s) 341
BshFI GGCC 5 cut(s) 140, 425, 579, 605, 928
BshNI GGYRCC 1 cut(s) 1115
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 341
BsiHKAI GWGCWC 1 cut(s) 966
BsiSI CCGG 5 cut(s) 137, 270, 737, 915, 941
BslFI GGGAC 2 cut(s) 201, 290
BslI CCNNNNNNNGG 3 cut(s) 224, 313, 845
BsmFI GGGAC 2 cut(s) 201, 290
BsnI GGCC 5 cut(s) 140, 425, 579, 605, 928
Bsp119I TTCGAA 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 966
Bsp13I TCCGGA 1 cut(s) 736
Bsp143I GATC 3 cut(s) 175, 392, 955
BspACI CCGC 5 cut(s) 173, 185, 341, 1098, 1142
BspANI GGCC 5 cut(s) 140, 425, 579, 605, 928
BspCNI CTCAG 2 cut(s) 888, 1030
BspDI ATCGAT 1 cut(s) 249
BspEI TCCGGA 1 cut(s) 736
BspLI GGNNCC 4 cut(s) 306, 394, 850, 1117
BspMAI CTGCAG 1 cut(s) 76
BspMI ACCTGC 2 cut(s) 141, 1010
BspPI GGATC 4 cut(s) 183, 387, 400, 963
BspT104I TTCGAA 1 cut(s) 368
BspT107I GGYRCC 1 cut(s) 1115
BsrBI CCGCTC 1 cut(s) 1098
BsrDI GCAATG 1 cut(s) 850
BsrFI RCCGGY 2 cut(s) 136, 914
BsrI ACTGG 2 cut(s) 382, 552
BssAI RCCGGY 2 cut(s) 136, 914
BssECI CCNNGG 3 cut(s) 100, 396, 700
BssMI GATC 3 cut(s) 175, 392, 955
BssT1I CCWWGG 2 cut(s) 100, 700
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 6 cut(s) 113, 155, 215, 313, 487, 807
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 7 cut(s) 72, 138, 163, 463, 916, 938, 1096
BstDEI CTNAG 4 cut(s) 617, 875, 1011, 1038
BstEII GGTNACC 1 cut(s) 508
BstF5I GGATG 6 cut(s) 88, 193, 406, 414, 661, 1048
BstHHI GCGC 1 cut(s) 1103
BstKTI GATC 3 cut(s) 178, 395, 958
BstMBI GATC 3 cut(s) 175, 392, 955
BstMCI CGRYCG 1 cut(s) 341
BstMWI GCNNNNNNNGC 4 cut(s) 133, 770, 1095, 1100
BstNI CCWGG 1 cut(s) 840
BstPI GGTNACC 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 838
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 152, 470, 773, 1115
BstV2I GAAGAC 2 cut(s) 204, 693
BstX2I RGATCY 2 cut(s) 392, 955
BstYI RGATCY 2 cut(s) 392, 955
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 5 cut(s) 140, 425, 579, 605, 928
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 6 cut(s) 88, 193, 406, 414, 661, 1048
BtsIMutI CAGTG 4 cut(s) 35, 375, 812, 1068
BveI ACCTGC 2 cut(s) 141, 1010
Cac8I GCNNGC 7 cut(s) 72, 138, 163, 463, 916, 938, 1096
CfoI GCGC 1 cut(s) 1103
Cfr10I RCCGGY 2 cut(s) 136, 914
Cfr13I GGNCC 4 cut(s) 139, 304, 848, 927
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 1 cut(s) 1082
Csp6I GTAC 1 cut(s) 826
CviAII CATG 3 cut(s) 193, 1126, 1138
CviQI GTAC 1 cut(s) 826
DdeI CTNAG 4 cut(s) 617, 875, 1011, 1038
DpnI GATC 3 cut(s) 177, 394, 957
DpnII GATC 3 cut(s) 175, 392, 955
DraIII CACNNNGTG 1 cut(s) 1082
EaeI YGGCCR 1 cut(s) 423
Eco130I CCWWGG 2 cut(s) 100, 700
Eco47I GGWCC 2 cut(s) 304, 848
Eco57I CTGAAG 2 cut(s) 700, 1022
Eco91I GGTNACC 1 cut(s) 508
EcoO65I GGTNACC 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 838
EcoT14I CCWWGG 2 cut(s) 100, 700
ErhI CCWWGG 2 cut(s) 100, 700
FaeI CATG 3 cut(s) 196, 1129, 1141
FalI AAGNNNNNCTT 2 cut(s) 678, 710
FaqI GGGAC 2 cut(s) 201, 290
FatI CATG 3 cut(s) 192, 1125, 1137
Fnu4HI GCNGC 6 cut(s) 166, 459, 762, 1099, 1104, 1143
FokI GGATG 6 cut(s) 95, 200, 413, 421, 648, 1055
Fsp4HI GCNGC 6 cut(s) 166, 459, 762, 1099, 1104, 1143
FspBI CTAG 6 cut(s) 170, 521, 588, 770, 794, 813
GlaI GCGC 1 cut(s) 1102
GluI GCNGC 6 cut(s) 166, 459, 762, 1099, 1104, 1143
GsaI CCCAGC 1 cut(s) 440
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 5 cut(s) 140, 425, 579, 605, 928
HapII CCGG 5 cut(s) 137, 270, 737, 915, 941
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 1 cut(s) 1103
Hin1II CATG 3 cut(s) 196, 1129, 1141
Hin6I GCGC 1 cut(s) 1101
HinP1I GCGC 1 cut(s) 1101
HincII GTYRAC 1 cut(s) 1065
HindII GTYRAC 1 cut(s) 1065
HindIII AAGCTT 4 cut(s) 445, 692, 731, 1087
HinfI GANTC 3 cut(s) 365, 1007, 1129
HpaII CCGG 5 cut(s) 137, 270, 737, 915, 941
HphI GGTGA 3 cut(s) 326, 502, 1063
Hpy166II GTNNAC 2 cut(s) 375, 1065
Hpy188I TCNGA 4 cut(s) 360, 397, 1002, 1039
Hpy188III TCNNGA 5 cut(s) 148, 737, 745, 859, 877
Hpy8I GTNNAC 2 cut(s) 375, 1065
HpyAV CCTTC 3 cut(s) 104, 616, 997
HpyCH4III ACNGT 6 cut(s) 113, 155, 215, 313, 487, 807
HpyCH4V TGCA 8 cut(s) 74, 161, 210, 243, 458, 563, 761, 1019
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 770, 1095, 1100
HpyF3I CTNAG 4 cut(s) 617, 875, 1011, 1038
Hsp92II CATG 3 cut(s) 196, 1129, 1141
HspAI GCGC 1 cut(s) 1101
Kpn2I TCCGGA 1 cut(s) 736
KroI GCCGGC 2 cut(s) 136, 914
KroNI GCCGGC 2 cut(s) 138, 916
Kzo9I GATC 3 cut(s) 175, 392, 955
LmnI GCTCC 1 cut(s) 986
Lsp1109I GCAGC 4 cut(s) 152, 470, 773, 1115
LweI GCATC 3 cut(s) 241, 840, 927
MaeI CTAG 6 cut(s) 170, 521, 588, 770, 794, 813
MaeIII GTNAC 2 cut(s) 113, 508
MalI GATC 3 cut(s) 177, 394, 957
MbiI CCGCTC 1 cut(s) 1098
MboI GATC 3 cut(s) 175, 392, 955
MboII GAAGA 5 cut(s) 209, 663, 695, 698, 760
MflI RGATCY 2 cut(s) 392, 955
MhlI GDGCHC 1 cut(s) 966
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 6 cut(s) 23, 180, 285, 325, 854, 863
MluNI TGGCCA 1 cut(s) 425
MnlI CCTC 7 cut(s) 79, 133, 152, 391, 549, 946, 1033
Mox20I TGGCCA 1 cut(s) 425
MroI TCCGGA 1 cut(s) 736
MroNI GCCGGC 2 cut(s) 136, 914
MscI TGGCCA 1 cut(s) 425
MseI TTAA 2 cut(s) 108, 453
MslI CAYNNNNRTG 1 cut(s) 1124
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 5 cut(s) 137, 270, 737, 915, 941
MspR9I CCNGG 1 cut(s) 840
MvaI CCWGG 1 cut(s) 840
MwoI GCNNNNNNNGC 4 cut(s) 133, 770, 1095, 1100
NaeI GCCGGC 2 cut(s) 138, 916
NdeII GATC 3 cut(s) 175, 392, 955
NgoMIV GCCGGC 2 cut(s) 136, 914
NlaIII CATG 3 cut(s) 196, 1129, 1141
NlaIV GGNNCC 4 cut(s) 306, 394, 850, 1117
NmuCI GTSAC 1 cut(s) 508
NspV TTCGAA 1 cut(s) 368
PaqCI CACCTGC 1 cut(s) 1010
PdiI GCCGGC 2 cut(s) 138, 916
PfeI GAWTC 3 cut(s) 365, 1007, 1129
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 6 cut(s) 167, 460, 763, 1100, 1105, 1144
Psp6I CCWGG 1 cut(s) 838
PspEI GGTNACC 1 cut(s) 508
PspFI CCCAGC 1 cut(s) 436
PspGI CCWGG 1 cut(s) 838
PspN4I GGNNCC 4 cut(s) 306, 394, 850, 1117
PspPI GGNCC 4 cut(s) 139, 304, 848, 927
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 2 cut(s) 392, 955
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 827
RsaNI GTAC 1 cut(s) 826
RseI CAYNNNNRTG 1 cut(s) 1124
SaqAI TTAA 2 cut(s) 108, 453
SatI GCNGC 6 cut(s) 166, 459, 762, 1099, 1104, 1143
Sau3AI GATC 3 cut(s) 175, 392, 955
Sau96I GGNCC 4 cut(s) 139, 304, 848, 927
ScrFI CCNGG 1 cut(s) 840
SduI GDGCHC 1 cut(s) 966
SfaNI GCATC 3 cut(s) 241, 840, 927
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 368
SinI GGWCC 2 cut(s) 304, 848
SmiMI CAYNNNNRTG 1 cut(s) 1124
SmlI CTYRAG 1 cut(s) 232
SmoI CTYRAG 1 cut(s) 232
Sse9I AATT 6 cut(s) 23, 180, 285, 325, 854, 863
SsiI CCGC 5 cut(s) 173, 185, 341, 1098, 1142
SspMI CTAG 6 cut(s) 170, 521, 588, 770, 794, 813
StyD4I CCNGG 1 cut(s) 838
StyI CCWWGG 2 cut(s) 100, 700
TaaI ACNGT 6 cut(s) 113, 155, 215, 313, 487, 807
TaqI TCGA 5 cut(s) 249, 368, 834, 867, 894
TasI AATT 6 cut(s) 23, 180, 285, 325, 854, 863
TatI WGTACW 1 cut(s) 825
TauI GCSGC 2 cut(s) 1101, 1145
TfiI GAWTC 3 cut(s) 365, 1007, 1129
Tru1I TTAA 2 cut(s) 108, 453
Tru9I TTAA 2 cut(s) 108, 453
TscAI CASTG 4 cut(s) 35, 382, 812, 1075
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 4 cut(s) 165, 458, 761, 1103
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 3 cut(s) 696, 1059, 1121
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 4 cut(s) 35, 382, 812, 1075
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 2 cut(s) 304, 848
XapI RAATTY 3 cut(s) 23, 854, 863
XspI CTAG 6 cut(s) 170, 521, 588, 770, 794, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.