MD04G1206600.v1.1

gpi-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005399
Physical Location & Seq
Reverse (-)
29229710 .. 29230662
953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1206600.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCCTCCTCCAAGCTAAGCTACTTTCTCTTTTATGTTCTTGGCTTACTCTTTCTTTCTCATCCAGTATTTTCAAACGATGAAGAAGACAAGCTTTTCCAAAGTCTCAACAACTTCAGGCAATCCCAAAAGCTTCCGGAGTTCAAGAAGAACGACAATGCAGCTTGTCTAGCCGATAAACTCGCCGACAAGCTAGAACACCGACAGTGTTCTAGCTCACAGAAGTACAGCATCGAGCCAGGCAACGGACCCAAATTCCCGAAATTCGACAAGCTCAGGAGAAGGTGCCATATCGAAACCAACTCGACAACAGCCGGCATCATTTGGCCCGTCTGCGTGCCGGATTTGGACGAGGATCTTGTGCTCAACAACTACACTCGCTCCCATTACGCAAAATATCTGAAGGATTCTAAGTATGCAGGTGTTGGGATTGGTTCTGAGGATGATTGGGTGATTGTTGTGTTGACCACTGACGCACAAAGTGGAAGCTTTGCGAGCGGCGCAGCCTCTTTGGGTGCCATTGGCATGATTCATTACATGGCGGCTGTTTTGGTGGGGTTGTTCTTGGTTTTGCTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

21.47

Weight (kDa)

6.51

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 29 - 155 2e-52 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 285, 515
AccBSI CCGCTC 1 cut(s) 498
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 2 cut(s) 498, 542
AclWI GGATC 1 cut(s) 363
AcsI RAATTY 2 cut(s) 254, 263
AcuI CTGAAG 2 cut(s) 100, 422
AdeI CACNNNGTG 1 cut(s) 482
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 2 cut(s) 75, 145
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 9 cut(s) 16, 21, 94, 133, 164, 193, 216, 274, 489
AluI AGCT 9 cut(s) 16, 21, 94, 133, 164, 193, 216, 274, 489
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 1 cut(s) 363
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 2 cut(s) 3, 326
ApeKI GCWGC 2 cut(s) 161, 503
ApoI RAATTY 2 cut(s) 254, 263
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 248, 327
AsuHPI GGTGA 1 cut(s) 463
AvaII GGWCC 1 cut(s) 248
BanI GGYRCC 2 cut(s) 285, 515
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 173, 515
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 3 cut(s) 170, 194, 213
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 4 cut(s) 162, 499, 504, 543
BlpI GCTNAGC 1 cut(s) 17
BlsI GCNGC 4 cut(s) 163, 500, 505, 544
Bme1390I CCNGG 1 cut(s) 240
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 2 cut(s) 248, 327
BmiI GGNNCC 3 cut(s) 250, 287, 517
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 2 cut(s) 240, 327
BpiI GAAGAC 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 275
Bpu1102I GCTNAGC 1 cut(s) 17
BsaWI WCCGGW 1 cut(s) 136
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 65
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 1 cut(s) 240
BseGI GGATG 2 cut(s) 61, 448
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMII CTCAG 2 cut(s) 289, 429
BseNI ACTGG 1 cut(s) 65
BseXI GCAGC 2 cut(s) 173, 515
BshFI GGCC 2 cut(s) 5, 328
BshNI GGYRCC 2 cut(s) 285, 515
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 3 cut(s) 137, 315, 341
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 110
BsnI GGCC 2 cut(s) 5, 328
Bsp1286I GDGCHC 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 1 cut(s) 355
Bsp1720I GCTNAGC 1 cut(s) 17
BspACI CCGC 2 cut(s) 498, 542
BspANI GGCC 2 cut(s) 5, 328
BspCNI CTCAG 2 cut(s) 288, 430
BspEI TCCGGA 1 cut(s) 136
BspLI GGNNCC 3 cut(s) 250, 287, 517
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 1 cut(s) 363
BspT107I GGYRCC 2 cut(s) 285, 515
BsrBI CCGCTC 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 314
BssMI GATC 1 cut(s) 355
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 207
BstC8I GCNNGC 3 cut(s) 316, 338, 496
BstDEI CTNAG 4 cut(s) 17, 275, 411, 438
BstF5I GGATG 2 cut(s) 61, 448
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 3 cut(s) 170, 495, 500
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 238
BstV1I GCAGC 2 cut(s) 173, 515
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 2 cut(s) 5, 328
BtsCI GGATG 2 cut(s) 61, 448
BtsIMutI CAGTG 2 cut(s) 212, 468
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 3 cut(s) 316, 338, 496
CfoI GCGC 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 2 cut(s) 248, 327
CseI GACGC 1 cut(s) 482
Csp6I GTAC 1 cut(s) 226
CviAII CATG 2 cut(s) 526, 538
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 4 cut(s) 17, 275, 411, 438
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
DraIII CACNNNGTG 1 cut(s) 482
Eco47I GGWCC 1 cut(s) 248
Eco57I CTGAAG 2 cut(s) 100, 422
EcoRII CCWGG 1 cut(s) 238
FaeI CATG 2 cut(s) 529, 541
FaiI YATR 5 cut(s) 36, 291, 417, 527, 539
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 2 cut(s) 525, 537
Fnu4HI GCNGC 4 cut(s) 162, 499, 504, 543
FokI GGATG 2 cut(s) 48, 455
Fsp4HI GCNGC 4 cut(s) 162, 499, 504, 543
FspBI CTAG 3 cut(s) 170, 194, 213
GlaI GCGC 1 cut(s) 502
GluI GCNGC 4 cut(s) 162, 499, 504, 543
HaeIII GGCC 2 cut(s) 5, 328
HapII CCGG 3 cut(s) 137, 315, 341
HgaI GACGC 1 cut(s) 482
HhaI GCGC 1 cut(s) 503
Hin1II CATG 2 cut(s) 529, 541
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 3 cut(s) 92, 131, 487
HinfI GANTC 2 cut(s) 407, 529
HpaII CCGG 3 cut(s) 137, 315, 341
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 1 cut(s) 465
Hpy188I TCNGA 2 cut(s) 402, 439
Hpy188III TCNNGA 4 cut(s) 137, 145, 259, 277
Hpy8I GTNNAC 1 cut(s) 465
HpyAV CCTTC 2 cut(s) 276, 397
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4V TGCA 2 cut(s) 161, 419
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 495, 500
HpyF3I CTNAG 4 cut(s) 17, 275, 411, 438
Hsp92II CATG 2 cut(s) 529, 541
HspAI GCGC 1 cut(s) 501
Kpn2I TCCGGA 1 cut(s) 136
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Kzo9I GATC 1 cut(s) 355
LmnI GCTCC 1 cut(s) 386
LpnPI CCDG 9 cut(s) 78, 103, 150, 225, 252, 262, 328, 354, 405
Lsp1109I GCAGC 2 cut(s) 173, 515
LweI GCATC 2 cut(s) 240, 327
MaeI CTAG 3 cut(s) 170, 194, 213
MalI GATC 1 cut(s) 357
MbiI CCGCTC 1 cut(s) 498
MboI GATC 1 cut(s) 355
MboII GAAGA 3 cut(s) 95, 98, 160
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 2 cut(s) 254, 263
MnlI CCTC 5 cut(s) 16, 19, 346, 433, 517
MroI TCCGGA 1 cut(s) 136
MroNI GCCGGC 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 3 cut(s) 137, 315, 341
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
MwoI GCNNNNNNNGC 3 cut(s) 170, 495, 500
NaeI GCCGGC 1 cut(s) 316
NdeII GATC 1 cut(s) 355
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 2 cut(s) 529, 541
NlaIV GGNNCC 3 cut(s) 250, 287, 517
PaqCI CACCTGC 1 cut(s) 410
PdiI GCCGGC 1 cut(s) 316
PfeI GAWTC 2 cut(s) 407, 529
PkrI GCNGC 4 cut(s) 163, 500, 505, 544
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 3 cut(s) 250, 287, 517
PspPI GGNCC 2 cut(s) 248, 327
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 524
SatI GCNGC 4 cut(s) 162, 499, 504, 543
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 2 cut(s) 248, 327
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 2 cut(s) 240, 327
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 524
Sse9I AATT 2 cut(s) 254, 263
SsiI CCGC 2 cut(s) 498, 542
SspMI CTAG 3 cut(s) 170, 194, 213
StyD4I CCNGG 1 cut(s) 238
TaaI ACNGT 1 cut(s) 207
TaqI TCGA 4 cut(s) 234, 267, 294, 305
TasI AATT 2 cut(s) 254, 263
TatI WGTACW 1 cut(s) 225
TauI GCSGC 2 cut(s) 501, 545
TfiI GAWTC 2 cut(s) 407, 529
TscAI CASTG 2 cut(s) 212, 475
TseI GCWGC 2 cut(s) 161, 503
TspDTI ATGAA 2 cut(s) 96, 521
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 2 cut(s) 212, 475
VpaK11BI GGWCC 1 cut(s) 248
XapI RAATTY 2 cut(s) 254, 263
XspI CTAG 3 cut(s) 170, 194, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.