MD12G1220400.v1.1

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005399
Physical Location & Seq
Reverse (-)
29726171 .. 29727097
927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1220400.v1.1.491

Sequence Viewer

Length: 630 bp
ATGGCCTCCTCCAAACTAAGCTACTTTCTCTTTGTTCTTGTTCTTGGCTTACTCTTCCTTTCTCATCCAGTATTTTCGAACGATGAAGAAGACGATCTTTTCCAAGGCCTCAACAGCTTCAGGAAATCCGCAAACCTGCCGGAATTTAAGAAGAACGACAATGCAGCTTGTCTAGCTGATGAACTCGCCGACGAGCTAGAAGACCAACCGTGTTCTAGCGCACACTACATCATCGAGCCAGGCAACTTACCCAGGTTCCCAAGCTTCGAGAAGCTCGTTAAAAAGTGCCATATCGACATCAACACGACAACAGCCGCCATCATTTGGCCTGTTTGCGTGCCGGATTTGGACGAGGATCTTGTGCTCAACAACTACACTCACTCCCATTCCTCAAAATATCTGAATGATTCCAAGTATTCAGGAGTTGGGATTGGTTCTGAGGATGATTGGATCGTTGCTGTGTTGACCACTGACGCGGAAAGTGGAAGCTTTTCTGGAGGCGCTGCTACTTTGGGTGCAATCGGCATGTTTCAATACATGGCGGCTGTTTTGTTGGGGTTGTTCTTGGTTTTAATGAACCAAAAATGCTTACAAAGACATTTCAGCCACCTCCCATCTATCATCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

23.01

Weight (kDa)

4.74

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 30 - 155 1.3e-52 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 324
AccII CGCG 1 cut(s) 476
AciI CCGC 4 cut(s) 129, 315, 476, 542
AclWI GGATC 2 cut(s) 363, 458
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 103
AfiI CCNNNNNNNGG 1 cut(s) 324
AgsI TTSAA 1 cut(s) 533
AjnI CCWGG 2 cut(s) 238, 251
AluBI AGCT 8 cut(s) 21, 117, 167, 176, 196, 264, 274, 489
AluI AGCT 8 cut(s) 21, 117, 167, 176, 196, 264, 274, 489
Alw21I GWGCWC 1 cut(s) 366
AlwI GGATC 2 cut(s) 363, 458
AoxI GGCC 3 cut(s) 3, 106, 326
ApeKI GCWGC 2 cut(s) 164, 503
ApoI RAATTY 1 cut(s) 143
Asp700I GAANNNNTTC 1 cut(s) 490
AspLEI GCGC 2 cut(s) 221, 503
AsuII TTCGAA 1 cut(s) 77
BbsI GAAGAC 2 cut(s) 96, 207
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 176, 490
BccI CCATC 2 cut(s) 326, 622
BciT130I CCWGG 2 cut(s) 240, 253
BfaI CTAG 3 cut(s) 173, 197, 216
BfoI RGCGCY 1 cut(s) 504
BfuAI ACCTGC 1 cut(s) 144
BisI GCNGC 4 cut(s) 165, 315, 504, 543
BlsI GCNGC 4 cut(s) 166, 316, 505, 544
Bme1390I CCNGG 2 cut(s) 240, 253
BmiI GGNNCC 1 cut(s) 257
BmrFI CCNGG 2 cut(s) 240, 253
BpiI GAAGAC 2 cut(s) 96, 207
BpmI CTGGAG 1 cut(s) 516
Bpu14I TTCGAA 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 103, 251
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 1 cut(s) 324
Bse1I ACTGG 1 cut(s) 68
BseBI CCWGG 2 cut(s) 240, 253
BseDI CCNNGG 2 cut(s) 103, 251
BseGI GGATG 2 cut(s) 64, 448
BseLI CCNNNNNNNGG 1 cut(s) 324
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 176, 490
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 3 cut(s) 5, 108, 328
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 2 cut(s) 140, 341
BslI CCNNNNNNNGG 1 cut(s) 324
BsnI GGCC 3 cut(s) 5, 108, 328
Bsp119I TTCGAA 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 3 cut(s) 94, 355, 450
BspACI CCGC 4 cut(s) 129, 315, 476, 542
BspANI GGCC 3 cut(s) 5, 108, 328
BspCNI CTCAG 1 cut(s) 430
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 1 cut(s) 257
BspMI ACCTGC 1 cut(s) 144
BspPI GGATC 2 cut(s) 363, 458
BspT104I TTCGAA 1 cut(s) 77
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 103, 251
BssMI GATC 3 cut(s) 94, 355, 450
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 2 cut(s) 240, 253
Bst4CI ACNGT 1 cut(s) 210
Bst6I CTCTTC 1 cut(s) 59
BstBI TTCGAA 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 2 cut(s) 17, 438
BstF5I GGATG 2 cut(s) 64, 448
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 2 cut(s) 221, 503
BstKTI GATC 3 cut(s) 97, 358, 453
BstMBI GATC 3 cut(s) 94, 355, 450
BstMWI GCNNNNNNNGC 2 cut(s) 114, 173
BstNI CCWGG 2 cut(s) 240, 253
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 2 cut(s) 238, 251
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 2 cut(s) 176, 490
BstV2I GAAGAC 2 cut(s) 96, 207
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 3 cut(s) 5, 108, 328
BtsCI GGATG 2 cut(s) 64, 448
BtsIMutI CAGTG 1 cut(s) 468
BveI ACCTGC 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 338
CfoI GCGC 2 cut(s) 221, 503
CseI GACGC 1 cut(s) 482
CviAII CATG 2 cut(s) 526, 538
DdeI CTNAG 2 cut(s) 17, 438
DpnI GATC 3 cut(s) 96, 357, 452
DpnII GATC 3 cut(s) 94, 355, 450
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 103
Eco147I AGGCCT 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 103
EcoRII CCWGG 2 cut(s) 238, 251
EcoT14I CCWWGG 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 2 cut(s) 529, 541
FaiI YATR 4 cut(s) 291, 527, 539, 628
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FatI CATG 2 cut(s) 525, 537
Fnu4HI GCNGC 4 cut(s) 165, 315, 504, 543
FokI GGATG 2 cut(s) 51, 455
Fsp4HI GCNGC 4 cut(s) 165, 315, 504, 543
FspBI CTAG 3 cut(s) 173, 197, 216
GlaI GCGC 2 cut(s) 220, 502
GluI GCNGC 4 cut(s) 165, 315, 504, 543
GsuI CTGGAG 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 3 cut(s) 5, 108, 328
HapII CCGG 2 cut(s) 140, 341
HgaI GACGC 1 cut(s) 482
HhaI GCGC 2 cut(s) 221, 503
Hin1II CATG 2 cut(s) 529, 541
Hin6I GCGC 2 cut(s) 219, 501
HinP1I GCGC 2 cut(s) 219, 501
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 2 cut(s) 262, 487
HinfI GANTC 1 cut(s) 407
HpaII CCGG 2 cut(s) 140, 341
Hpy166II GTNNAC 1 cut(s) 465
Hpy188I TCNGA 2 cut(s) 402, 439
Hpy188III TCNNGA 4 cut(s) 121, 268, 420, 495
Hpy8I GTNNAC 1 cut(s) 465
Hpy99I CGWCG 1 cut(s) 194
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 164, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 173
HpyF3I CTNAG 2 cut(s) 17, 438
Hsp92II CATG 2 cut(s) 529, 541
HspAI GCGC 2 cut(s) 219, 501
Kzo9I GATC 3 cut(s) 94, 355, 450
Lsp1109I GCAGC 2 cut(s) 176, 490
MaeI CTAG 3 cut(s) 173, 197, 216
MalI GATC 3 cut(s) 96, 357, 452
MboI GATC 3 cut(s) 94, 355, 450
MboII GAAGA 5 cut(s) 46, 98, 101, 163, 212
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 1 cut(s) 143
MnlI CCTC 8 cut(s) 16, 19, 119, 346, 400, 433, 491, 620
MroXI GAANNNNTTC 1 cut(s) 490
MseI TTAA 3 cut(s) 147, 279, 572
MspI CCGG 2 cut(s) 140, 341
MspR9I CCNGG 2 cut(s) 240, 253
MvaI CCWGG 2 cut(s) 240, 253
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 2 cut(s) 114, 173
NdeII GATC 3 cut(s) 94, 355, 450
NlaIII CATG 2 cut(s) 529, 541
NlaIV GGNNCC 1 cut(s) 257
NspI RCATGY 1 cut(s) 529
NspV TTCGAA 1 cut(s) 77
PceI AGGCCT 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 490
PfeI GAWTC 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 324
PkrI GCNGC 4 cut(s) 166, 316, 505, 544
Psp6I CCWGG 2 cut(s) 238, 251
PspGI CCWGG 2 cut(s) 238, 251
PspN4I GGNNCC 1 cut(s) 257
PsuI RGATCY 1 cut(s) 355
SaqAI TTAA 3 cut(s) 147, 279, 572
SatI GCNGC 4 cut(s) 165, 315, 504, 543
Sau3AI GATC 3 cut(s) 94, 355, 450
ScrFI CCNGG 2 cut(s) 240, 253
SduI GDGCHC 1 cut(s) 366
SfuI TTCGAA 1 cut(s) 77
Sse9I AATT 1 cut(s) 143
SseBI AGGCCT 1 cut(s) 108
SsiI CCGC 4 cut(s) 129, 315, 476, 542
SspMI CTAG 3 cut(s) 173, 197, 216
StuI AGGCCT 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 238, 251
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 1 cut(s) 210
TaqI TCGA 4 cut(s) 77, 234, 267, 294
TasI AATT 1 cut(s) 143
TauI GCSGC 2 cut(s) 317, 545
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 3 cut(s) 147, 279, 572
Tru9I TTAA 3 cut(s) 147, 279, 572
TscAI CASTG 1 cut(s) 475
TseI GCWGC 2 cut(s) 164, 503
TspDTI ATGAA 3 cut(s) 99, 195, 590
TspRI CASTG 1 cut(s) 475
Van91I CCANNNNNTGG 1 cut(s) 324
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 529
XmnI GAANNNNTTC 1 cut(s) 490
XspI CTAG 3 cut(s) 173, 197, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.