RLG00000025435

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005399
Physical Location & Seq
Reverse (-)
45757595 .. 45758851
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025435

Sequence Viewer

Length: 603 bp
ATGGCCTCCCTTATCAAACTTTGCTTCTTCTTCATTGTGATTATTGAAGCCTCTCTCTTGCTTTCACCCTTGGTGCACAGTGATGATGAAGCCAATGAAGATGATATTCTCCAAGGCATCAACAGCTATAGACGTTCACTGAGCTTACCGAACCTCATCAAGCACGACAAAGCCGATTGCCTAGCAGAAGAAATCGCTGATGATATGCAGGACCAACCATGCAGCAGCAGAACCAATGGCGCCAACATCTCACCAACATCTGTGACTGAACTCCCCAGTCTTCCCAGTAACTTGATCAAGTGCAAAATAGATGTGAACAGCACAGTGGATGGGGTCATAATGCCAGTTTGTGTGCCCAAAGCAGTCTCAACTCTTGTGCTTACCAATTACACTAGCTCCCCGCGCTACGCAAAATTTCTGAATGATTCGAGGTTCACTGGGGTTGGAATTGGAACCGAGGACGATTGGACTGTGGTGGTTTTGGCCACAAGTACCCGAACAGGAAGTTTTGCCAATGCAGCAAACTCTTTGGTTTTGACGGTTGGCGCTATCCATTACTTGGTCTCCTTAATGTTGGGGTTGTGGCTTATTTTGGTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

21.59

Weight (kDa)

4.7

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 33 - 161 2.1e-51 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 239
AccB7I CCANNNNNTGG 1 cut(s) 559
AccII CGCG 1 cut(s) 403
AciI CCGC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 1 cut(s) 413
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 1 cut(s) 47
AluBI AGCT 4 cut(s) 126, 144, 396, 600
AluI AGCT 4 cut(s) 126, 144, 396, 600
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 2 cut(s) 370, 568
Alw44I GTGCAC 1 cut(s) 74
AoxI GGCC 2 cut(s) 3, 483
ApaLI GTGCAC 1 cut(s) 74
ApeKI GCWGC 3 cut(s) 222, 225, 518
ApoI RAATTY 1 cut(s) 413
AspLEI GCGC 3 cut(s) 242, 405, 548
AspS9I GGNCC 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 57, 243
AvaII GGWCC 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 78, 357
BalI TGGCCA 1 cut(s) 485
BanI GGYRCC 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 272
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 234, 237, 530
BccI CCATC 1 cut(s) 323
BclI TGATCA 1 cut(s) 294
BcoDI GTCTC 2 cut(s) 370, 568
BfaI CTAG 2 cut(s) 182, 393
BfmI CTRYAG 1 cut(s) 127
BfoI RGCGCY 2 cut(s) 243, 549
BisI GCNGC 3 cut(s) 223, 226, 519
BlsI GCNGC 3 cut(s) 224, 227, 520
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 241, 454
BmrI ACTGGG 3 cut(s) 270, 279, 447
BmsI GCATC 1 cut(s) 126
BmuI ACTGGG 3 cut(s) 270, 279, 447
BpiI GAAGAC 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 3 cut(s) 69, 112, 456
BsaXI ACNNNNNCTCC 4 cut(s) 380, 410, 548, 578
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse1I ACTGG 4 cut(s) 276, 285, 344, 442
BseDI CCNNGG 3 cut(s) 69, 112, 456
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 4 cut(s) 276, 285, 344, 442
BseSI GKGCMC 2 cut(s) 78, 357
BseXI GCAGC 3 cut(s) 234, 237, 530
Bsh1236I CGCG 1 cut(s) 403
BshFI GGCC 2 cut(s) 5, 485
BshNI GGYRCC 1 cut(s) 239
BsiHKAI GWGCWC 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 2 cut(s) 370, 568
BsnI GGCC 2 cut(s) 5, 485
Bso31I GGTCTC 1 cut(s) 568
Bsp1286I GDGCHC 2 cut(s) 78, 357
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 1 cut(s) 401
BspANI GGCC 2 cut(s) 5, 485
BspCNI CTCAG 1 cut(s) 132
BspFNI CGCG 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 241, 454
BspT107I GGYRCC 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 568
BsrI ACTGG 4 cut(s) 276, 285, 344, 442
BssECI CCNNGG 3 cut(s) 69, 112, 456
BssMI GATC 1 cut(s) 294
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 69, 112
Bst4CI ACNGT 4 cut(s) 80, 325, 472, 541
BstACI GRCGYC 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 403
BstH2I RGCGCY 2 cut(s) 243, 549
BstHHI GCGC 3 cut(s) 242, 405, 548
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 2 cut(s) 370, 568
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 3 cut(s) 123, 402, 518
BstSFI CTRYAG 1 cut(s) 127
BstSLI GKGCMC 2 cut(s) 78, 357
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 3 cut(s) 234, 237, 530
BstV2I GAAGAC 1 cut(s) 272
BsuRI GGCC 2 cut(s) 5, 485
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 4 cut(s) 85, 137, 330, 435
CfoI GCGC 3 cut(s) 242, 405, 548
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 1 cut(s) 492
CviAII CATG 1 cut(s) 219
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 1 cut(s) 140
DinI GGCGCC 1 cut(s) 241
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 483
Eco130I CCWWGG 2 cut(s) 69, 112
Eco31I GGTCTC 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 211
EcoT14I CCWWGG 2 cut(s) 69, 112
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
ErhI CCWWGG 2 cut(s) 69, 112
FaeI CATG 1 cut(s) 222
FaiI YATR 4 cut(s) 129, 206, 220, 338
FatI CATG 1 cut(s) 218
FauI CCCGC 1 cut(s) 408
FbaI TGATCA 1 cut(s) 294
Fnu4HI GCNGC 3 cut(s) 223, 226, 519
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 3 cut(s) 223, 226, 519
FspBI CTAG 2 cut(s) 182, 393
GlaI GCGC 3 cut(s) 241, 404, 547
GluI GCNGC 3 cut(s) 223, 226, 519
HaeII RGCGCY 2 cut(s) 243, 549
HaeIII GGCC 2 cut(s) 5, 485
HhaI GCGC 3 cut(s) 242, 405, 548
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 1 cut(s) 222
Hin6I GCGC 3 cut(s) 240, 403, 546
HinP1I GCGC 3 cut(s) 240, 403, 546
HinfI GANTC 1 cut(s) 425
HphI GGTGA 2 cut(s) 57, 243
Hpy166II GTNNAC 4 cut(s) 76, 137, 316, 435
Hpy188I TCNGA 1 cut(s) 420
Hpy8I GTNNAC 4 cut(s) 76, 137, 316, 435
HpyCH4III ACNGT 4 cut(s) 80, 325, 472, 541
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 5 cut(s) 76, 208, 222, 303, 518
HpyF10VI GCNNNNNNNGC 3 cut(s) 123, 402, 518
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 133
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 1 cut(s) 222
HspAI GCGC 3 cut(s) 240, 403, 546
KasI GGCGCC 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 294
Kzo9I GATC 1 cut(s) 294
LmnI GCTCC 1 cut(s) 401
LpnPI CCDG 6 cut(s) 194, 289, 298, 357, 423, 486
Lsp1109I GCAGC 3 cut(s) 234, 237, 530
LweI GCATC 1 cut(s) 126
MaeI CTAG 2 cut(s) 182, 393
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 2 cut(s) 262, 287
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 5 cut(s) 19, 22, 110, 200, 272
MhlI GDGCHC 2 cut(s) 78, 357
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 3 cut(s) 385, 413, 447
MluNI TGGCCA 1 cut(s) 485
Mly113I GGCGCC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 5 cut(s) 16, 61, 164, 423, 451
Mox20I TGGCCA 1 cut(s) 485
MscI TGGCCA 1 cut(s) 485
MseI TTAA 1 cut(s) 569
MslI CAYNNNNRTG 1 cut(s) 81
Msp20I TGGCCA 1 cut(s) 485
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 3 cut(s) 123, 402, 518
NarI GGCGCC 1 cut(s) 240
NdeII GATC 1 cut(s) 294
NlaIII CATG 1 cut(s) 222
NlaIV GGNNCC 2 cut(s) 241, 454
NmuCI GTSAC 1 cut(s) 262
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 559
PkrI GCNGC 3 cut(s) 224, 227, 520
PluTI GGCGCC 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 241, 454
PspPI GGNCC 1 cut(s) 211
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 1 cut(s) 81
SaqAI TTAA 1 cut(s) 569
SatI GCNGC 3 cut(s) 223, 226, 519
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 1 cut(s) 211
SduI GDGCHC 2 cut(s) 78, 357
SetI ASST 7 cut(s) 128, 136, 146, 156, 398, 434, 602
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 127
SfoI GGCGCC 1 cut(s) 241
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 81
Sse9I AATT 3 cut(s) 385, 413, 447
SsiI CCGC 1 cut(s) 401
SspDI GGCGCC 1 cut(s) 239
SspMI CTAG 2 cut(s) 182, 393
StyI CCWWGG 2 cut(s) 69, 112
TaaI ACNGT 4 cut(s) 80, 325, 472, 541
TaiI ACGT 1 cut(s) 136
TaqI TCGA 1 cut(s) 428
TasI AATT 3 cut(s) 385, 413, 447
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 1 cut(s) 569
Tru9I TTAA 1 cut(s) 569
TscAI CASTG 4 cut(s) 85, 144, 330, 442
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 3 cut(s) 222, 225, 518
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 3 cut(s) 22, 102, 111
TspRI CASTG 4 cut(s) 85, 144, 330, 442
Van91I CCANNNNNTGG 1 cut(s) 559
VneI GTGCAC 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 1 cut(s) 413
XspI CTAG 2 cut(s) 182, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.