Rw3G004640

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005399
Physical Location & Seq
Forward (+)
3981708 .. 3982952
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004640.1

Sequence Viewer

Length: 603 bp
ATGGCCTCCCTTATCAAACTTTGCTTCTTCTTCATTGTGATTATTCAAGCCTCTCTCTTGCTTTCACCTTTGGTGCTCAGTGATGATGAAGCCAATGAAGATGATATTCTCCAAGGCATCAACAGCTACAGACGATCACTGAGCTTACCGAACCTCATCAAGCACGACAAAGCGGATTGCCTAGCAGAAGAAATTGCTGATGATATACAGGACCAACCATGCAGCAGCCGAACCAATGGCGCCAACATCTCACCAACATCTGCGACTGAACTCCCCAGTCTTCCCAGTAACTTGATCAAGTGCAAAATAGATGTGAACAGCACAGTGGATGGGGTCATAATGCCAGTTTGTGTGCCCAAAGCAGTCTCAACTCTTGTGCTTACCAATTACACTAGCTCCCCGCGGTACGCAAAATTTCTGAATGATTCGAGGTTCACTGGGGTTGGAATTGGTACCGAGGACGATTGGACTGTGGTGGTTTTGGCAACAAGTACCCGAACAGGAACCTTTGCCAATGCAGCAAACTCTTTGGTTTCGACGGTTGGCGCTGTCCATTACTTGGTCTCCTTAATGTTGGGGTTGTGGCTTATTTTGGTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

21.49

Weight (kDa)

4.66

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 33 - 161 1.8e-51 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 452
AccB1I GGYRCC 2 cut(s) 239, 452
AccB7I CCANNNNNTGG 1 cut(s) 559
AccII CGCG 1 cut(s) 403
AciI CCGC 3 cut(s) 173, 401, 403
AcsI RAATTY 1 cut(s) 413
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 3 cut(s) 407, 454, 493
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 1 cut(s) 47
AluBI AGCT 4 cut(s) 126, 144, 396, 600
AluI AGCT 4 cut(s) 126, 144, 396, 600
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 2 cut(s) 370, 568
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 222, 225, 518
ApoI RAATTY 1 cut(s) 413
Asp718I GGTACC 1 cut(s) 452
AspLEI GCGC 2 cut(s) 242, 548
AspS9I GGNCC 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 57, 243
AvaII GGWCC 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 357
BanI GGYRCC 2 cut(s) 239, 452
BbsI GAAGAC 1 cut(s) 272
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 234, 237, 530
BccI CCATC 1 cut(s) 323
BclI TGATCA 1 cut(s) 294
BcoDI GTCTC 2 cut(s) 370, 568
BfaI CTAG 2 cut(s) 182, 393
BfmI CTRYAG 1 cut(s) 127
BfoI RGCGCY 2 cut(s) 243, 549
BisI GCNGC 3 cut(s) 223, 226, 519
BlsI GCNGC 3 cut(s) 224, 227, 520
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 3 cut(s) 241, 454, 505
BmrI ACTGGG 3 cut(s) 270, 279, 447
BmsI GCATC 1 cut(s) 126
BmuI ACTGGG 3 cut(s) 270, 279, 447
BpiI GAAGAC 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 3 cut(s) 112, 401, 456
BsaXI ACNNNNNCTCC 4 cut(s) 380, 410, 548, 578
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse1I ACTGG 4 cut(s) 276, 285, 344, 442
BseDI CCNNGG 3 cut(s) 112, 401, 456
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMII CTCAG 2 cut(s) 91, 131
BseNI ACTGG 4 cut(s) 276, 285, 344, 442
BseSI GKGCMC 1 cut(s) 357
BseXI GCAGC 3 cut(s) 234, 237, 530
Bsh1236I CGCG 1 cut(s) 403
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 239, 452
BsiHKAI GWGCWC 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 2 cut(s) 370, 568
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 568
Bsp1286I GDGCHC 2 cut(s) 78, 357
Bsp143I GATC 2 cut(s) 134, 294
BspACI CCGC 3 cut(s) 173, 401, 403
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 90, 132
BspFNI CGCG 1 cut(s) 403
BspLI GGNNCC 3 cut(s) 241, 454, 505
BspT107I GGYRCC 2 cut(s) 239, 452
BspTNI GGTCTC 1 cut(s) 568
BsrI ACTGG 4 cut(s) 276, 285, 344, 442
BssECI CCNNGG 3 cut(s) 112, 401, 456
BssMI GATC 2 cut(s) 134, 294
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 3 cut(s) 325, 472, 541
BstACI GRCGYC 1 cut(s) 240
BstDEI CTNAG 2 cut(s) 77, 140
BstDSI CCRYGG 1 cut(s) 401
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 403
BstH2I RGCGCY 2 cut(s) 243, 549
BstHHI GCGC 2 cut(s) 242, 548
BstKTI GATC 2 cut(s) 137, 297
BstMAI GTCTC 2 cut(s) 370, 568
BstMBI GATC 2 cut(s) 134, 294
BstMWI GCNNNNNNNGC 2 cut(s) 123, 518
BstSFI CTRYAG 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 357
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 3 cut(s) 234, 237, 530
BstV2I GAAGAC 1 cut(s) 272
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 401
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 4 cut(s) 85, 137, 330, 435
CfoI GCGC 2 cut(s) 242, 548
Cfr13I GGNCC 1 cut(s) 211
Cfr42I CCGCGG 1 cut(s) 404
Csp6I GTAC 3 cut(s) 406, 453, 492
CviAII CATG 1 cut(s) 219
CviJI RGCY 9 cut(s) 5, 50, 92, 126, 144, 228, 396, 586, 600
CviKI_1 RGCY 9 cut(s) 5, 50, 92, 126, 144, 228, 396, 586, 600
CviQI GTAC 3 cut(s) 406, 453, 492
DdeI CTNAG 2 cut(s) 77, 140
DinI GGCGCC 1 cut(s) 241
DpnI GATC 2 cut(s) 136, 296
DpnII GATC 2 cut(s) 134, 294
Eco130I CCWWGG 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 112
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 1 cut(s) 222
FaiI YATR 3 cut(s) 206, 220, 338
FatI CATG 1 cut(s) 218
FauI CCCGC 1 cut(s) 408
FbaI TGATCA 1 cut(s) 294
Fnu4HI GCNGC 3 cut(s) 223, 226, 519
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 3 cut(s) 223, 226, 519
FspBI CTAG 2 cut(s) 182, 393
GlaI GCGC 2 cut(s) 241, 547
GluI GCNGC 3 cut(s) 223, 226, 519
HaeII RGCGCY 2 cut(s) 243, 549
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 2 cut(s) 242, 548
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 1 cut(s) 222
Hin6I GCGC 2 cut(s) 240, 546
HinP1I GCGC 2 cut(s) 240, 546
HinfI GANTC 1 cut(s) 425
HphI GGTGA 2 cut(s) 57, 243
Hpy166II GTNNAC 2 cut(s) 316, 435
Hpy188I TCNGA 1 cut(s) 420
Hpy8I GTNNAC 2 cut(s) 316, 435
Hpy99I CGWCG 1 cut(s) 541
HpyCH4III ACNGT 3 cut(s) 325, 472, 541
HpyCH4V TGCA 3 cut(s) 222, 303, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 518
HpyF3I CTNAG 2 cut(s) 77, 140
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 1 cut(s) 222
HspAI GCGC 2 cut(s) 240, 546
KasI GGCGCC 1 cut(s) 239
KpnI GGTACC 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 294
KspI CCGCGG 1 cut(s) 404
Kzo9I GATC 2 cut(s) 134, 294
LmnI GCTCC 1 cut(s) 401
LpnPI CCDG 6 cut(s) 194, 289, 298, 357, 423, 486
Lsp1109I GCAGC 3 cut(s) 234, 237, 530
LweI GCATC 1 cut(s) 126
MaeI CTAG 2 cut(s) 182, 393
MaeIII GTNAC 1 cut(s) 287
MalI GATC 2 cut(s) 136, 296
MboI GATC 2 cut(s) 134, 294
MboII GAAGA 5 cut(s) 19, 22, 110, 200, 272
MhlI GDGCHC 2 cut(s) 78, 357
MluCI AATT 4 cut(s) 192, 385, 413, 447
Mly113I GGCGCC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 5 cut(s) 16, 61, 164, 423, 451
MseI TTAA 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 403
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 2 cut(s) 123, 518
NarI GGCGCC 1 cut(s) 240
NdeII GATC 2 cut(s) 134, 294
NlaIII CATG 1 cut(s) 222
NlaIV GGNNCC 3 cut(s) 241, 454, 505
PfeI GAWTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 559
PkrI GCNGC 3 cut(s) 224, 227, 520
PluTI GGCGCC 1 cut(s) 243
PspN4I GGNNCC 3 cut(s) 241, 454, 505
PspPI GGNCC 1 cut(s) 211
RsaI GTAC 3 cut(s) 407, 454, 493
RsaNI GTAC 3 cut(s) 406, 453, 492
SacII CCGCGG 1 cut(s) 404
SaqAI TTAA 1 cut(s) 569
SatI GCNGC 3 cut(s) 223, 226, 519
Sau3AI GATC 2 cut(s) 134, 294
Sau96I GGNCC 1 cut(s) 211
SduI GDGCHC 2 cut(s) 78, 357
SetI ASST 8 cut(s) 70, 128, 146, 156, 398, 434, 509, 602
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 127
SfoI GGCGCC 1 cut(s) 241
Sfr303I CCGCGG 1 cut(s) 404
SgrBI CCGCGG 1 cut(s) 404
SinI GGWCC 1 cut(s) 211
Sse9I AATT 4 cut(s) 192, 385, 413, 447
SsiI CCGC 3 cut(s) 173, 401, 403
SspDI GGCGCC 1 cut(s) 239
SspMI CTAG 2 cut(s) 182, 393
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 3 cut(s) 325, 472, 541
TaqI TCGA 2 cut(s) 428, 536
TasI AATT 4 cut(s) 192, 385, 413, 447
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 1 cut(s) 569
Tru9I TTAA 1 cut(s) 569
TscAI CASTG 4 cut(s) 85, 144, 330, 442
TseI GCWGC 3 cut(s) 222, 225, 518
TspDTI ATGAA 3 cut(s) 22, 102, 111
TspRI CASTG 4 cut(s) 85, 144, 330, 442
Van91I CCANNNNNTGG 1 cut(s) 559
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 1 cut(s) 413
XspI CTAG 2 cut(s) 182, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.