FvH4_6g05670

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005399
Physical Location & Seq
Forward (+)
3230415 .. 3232923
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05670.t1

Sequence Viewer

Length: 1200 bp
ATGGCCTCCCTTATCAAACTTTGCTTCTTCTTCCTTCTGTTCATTCAAGCTACTCTCTTACTTTCACCCTTGGTGCACAGTGATCGTGAGGCCAATGAAGATGATGTTCTCCAAGGCATCAATAGCTACAGACGATCACTAAGCTTACCGGCCCTAATCAAGCACGACAAAGCCGATTGCCTAGCAGGAGAGATTGCTGATGATATAAACGATCAACCATGCAGCAGCCGAACCAATGGTGCCAACATCTCTCCAGCATCTGTGTCAACTGACCTTCCCAGTCTTCCAAGTAACTTGATTAAGTGCAAAATAGATGTGAACAGCACAGTGGATGGGGTCATCATGCCAGTTTGTGTTCCCAAAGCGGTCGCAACTCTTGTGCTTACCAACTATACTAGTTCCCCACACTACGCGAAGTTTCTGAATGATTCGAGGTTCACTGGGGTTGGAGTTGGTACCGAGGATGATTGGACTGTGGTGGTTTTGGCCACAAGTACCCGAACAGGAAGCTTTGCCAATGCATCACTCTTTGGTTTCGAGGGTTTCCACAGTTCATTACTTGGTTTCCTTCATGTTGTGGCTTATTTTTCTTGGCTGAACCTTGTGCATAGAAAACATATGGCCTCGTCAAGACTGAGCTTGTTTGCCTTTGTGCTTGTTCATGCCTTCCTCCTCCATTCTTCTCTAGTATGTTGTAATGATGAAGAAGACGACCTTTTTGTCGGCCTCAACAATTTCAGGCAAACCCTAAACCTCTCAGAACTCGTAAAGAACGAGAAGGCCGGCTGTATCGCCGACGAGATCTCAGATGAGCTACAGGGCCTACCATGCTCTCATGCAGCTGACTATGAGATTAAACCAGGCAATGGTCCTGTGTTCCCCTACTTCAGCAAGCTCATAACCAAGTGTAACATAGACAACAGCACCACAACTGATGGGATCATTTTGCCTGTTTGCGTGCCCGATTTGGACCCAGATCTTGTGCTTAACAATTATACTAAAACTAAATTTGCTCAGTTTCTGAATAATTCTAAGTATACAGGAGCTGGGGTTGGTTCTGAGGATGATTGGATGATTGTTGTGTTGACTACTGACTCACCTAGTGGGAGCTTTACTGGTGGTGCTGCTTCTTTGGCTGCAATTGGCATGGTTCACTACATGGTTGCTTTGTTCTTGGGATTGTCCTTCATTTTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

43.2

Weight (kDa)

4.97

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 33 - 162 1.8e-48 Uncharacterized GPI-anchored protein At5g19230-like domain
At5g19230 PF25884 236 - 362 1.1e-49 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 719
Acc65I GGTACC 1 cut(s) 455
AccB1I GGYRCC 2 cut(s) 239, 455
AccB7I CCANNNNNTGG 1 cut(s) 866
AccI GTMKAC 1 cut(s) 1037
AccII CGCG 1 cut(s) 413
AciI CCGC 1 cut(s) 365
AclWI GGATC 1 cut(s) 947
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 1 cut(s) 1007
AcuI CTGAAG 1 cut(s) 871
AdeI CACNNNGTG 1 cut(s) 1103
AfaI GTAC 2 cut(s) 457, 496
AfiI CCNNNNNNNGG 1 cut(s) 866
AgsI TTSAA 1 cut(s) 47
AhlI ACTAGT 1 cut(s) 395
AjnI CCWGG 1 cut(s) 859
Alw21I GWGCWC 1 cut(s) 78
Alw44I GTGCAC 1 cut(s) 74
AlwI GGATC 1 cut(s) 947
AlwNI CAGNNNCTG 3 cut(s) 260, 1021, 1046
AoxI GGCC 8 cut(s) 3, 90, 150, 486, 621, 724, 780, 820
ApaLI GTGCAC 1 cut(s) 74
ApeKI GCWGC 5 cut(s) 222, 225, 839, 1124, 1136
ApoI RAATTY 1 cut(s) 1007
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
Asp718I GGTACC 1 cut(s) 455
AspS9I GGNCC 4 cut(s) 151, 820, 869, 970
AsuHPI GGTGA 2 cut(s) 57, 1089
AvaII GGWCC 2 cut(s) 869, 970
BaeGI GKGCMC 2 cut(s) 78, 963
BalI TGGCCA 1 cut(s) 488
BanI GGYRCC 2 cut(s) 239, 455
BbsI GAAGAC 2 cut(s) 275, 714
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 5 cut(s) 234, 237, 851, 1111, 1123
BccI CCATC 2 cut(s) 326, 929
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 1 cut(s) 861
BcuI ACTAGT 1 cut(s) 395
BfaI CTAG 4 cut(s) 182, 396, 686, 1101
BfmI CTRYAG 2 cut(s) 127, 815
BglII AGATCT 2 cut(s) 801, 976
BisI GCNGC 5 cut(s) 223, 226, 840, 1125, 1137
BlsI GCNGC 5 cut(s) 224, 227, 841, 1126, 1138
Bme1390I CCNGG 1 cut(s) 861
Bme18I GGWCC 2 cut(s) 869, 970
BmgT120I GGNCC 4 cut(s) 151, 820, 869, 970
BmiI GGNNCC 3 cut(s) 241, 457, 972
BmrFI CCNGG 1 cut(s) 861
BmrI ACTGGG 2 cut(s) 273, 450
BmsI GCATC 3 cut(s) 126, 266, 530
BmuI ACTGGG 2 cut(s) 273, 450
BpiI GAAGAC 2 cut(s) 275, 714
BpmI CTGGAG 1 cut(s) 237
BsaJI CCNNGG 3 cut(s) 69, 112, 459
BsaXI ACNNNNNCTCC 2 cut(s) 1035, 1065
Bsc4I CCNNNNNNNGG 1 cut(s) 866
Bse118I RCCGGY 2 cut(s) 148, 782
Bse1I ACTGG 4 cut(s) 279, 347, 445, 1120
Bse3DI GCAATG 1 cut(s) 871
BseBI CCWGG 1 cut(s) 861
BseDI CCNNGG 3 cut(s) 69, 112, 459
BseGI GGATG 4 cut(s) 337, 469, 1069, 1077
BseLI CCNNNNNNNGG 1 cut(s) 866
BseMI GCAATG 1 cut(s) 871
BseMII CTCAG 5 cut(s) 626, 771, 819, 1028, 1050
BseNI ACTGG 4 cut(s) 279, 347, 445, 1120
BseRI GAGGAG 1 cut(s) 662
BseSI GKGCMC 2 cut(s) 78, 963
BseXI GCAGC 5 cut(s) 234, 237, 851, 1111, 1123
BseYI CCCAGC 1 cut(s) 1046
Bsh1236I CGCG 1 cut(s) 413
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 8 cut(s) 5, 92, 152, 488, 623, 726, 782, 822
BshNI GGYRCC 2 cut(s) 239, 455
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 2 cut(s) 149, 783
BslI CCNNNNNNNGG 1 cut(s) 866
BsnI GGCC 8 cut(s) 5, 92, 152, 488, 623, 726, 782, 822
Bsp1286I GDGCHC 2 cut(s) 78, 963
Bsp143I GATC 6 cut(s) 82, 134, 211, 801, 939, 976
BspACI CCGC 1 cut(s) 365
BspANI GGCC 8 cut(s) 5, 92, 152, 488, 623, 726, 782, 822
BspCNI CTCAG 5 cut(s) 627, 770, 818, 1027, 1051
BspFNI CGCG 1 cut(s) 413
BspLI GGNNCC 3 cut(s) 241, 457, 972
BspPI GGATC 1 cut(s) 947
BspT107I GGYRCC 2 cut(s) 239, 455
BsrDI GCAATG 1 cut(s) 871
BsrFI RCCGGY 2 cut(s) 148, 782
BsrI ACTGG 4 cut(s) 279, 347, 445, 1120
BssAI RCCGGY 2 cut(s) 148, 782
BssECI CCNNGG 3 cut(s) 69, 112, 459
BssMI GATC 6 cut(s) 82, 134, 211, 801, 939, 976
BssNAI GTATAC 1 cut(s) 1038
BssT1I CCWWGG 2 cut(s) 69, 112
Bst1107I GTATAC 1 cut(s) 1038
Bst2UI CCWGG 1 cut(s) 861
Bst4CI ACNGT 4 cut(s) 80, 328, 475, 551
BstC8I GCNNGC 3 cut(s) 784, 893, 959
BstDEI CTNAG 7 cut(s) 140, 635, 757, 805, 1014, 1032, 1059
BstF5I GGATG 4 cut(s) 337, 469, 1069, 1077
BstFNI CGCG 1 cut(s) 413
BstKTI GATC 6 cut(s) 85, 137, 214, 804, 942, 979
BstMBI GATC 6 cut(s) 82, 134, 211, 801, 939, 976
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 3 cut(s) 123, 828, 1133
BstNI CCWGG 1 cut(s) 861
BstSCI CCNGG 1 cut(s) 859
BstSFI CTRYAG 2 cut(s) 127, 815
BstSLI GKGCMC 2 cut(s) 78, 963
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 5 cut(s) 234, 237, 851, 1111, 1123
BstV2I GAAGAC 2 cut(s) 275, 714
BstX2I RGATCY 2 cut(s) 801, 976
BstYI RGATCY 2 cut(s) 801, 976
BstZ17I GTATAC 1 cut(s) 1038
BsuRI GGCC 8 cut(s) 5, 92, 152, 488, 623, 726, 782, 822
BtsCI GGATG 4 cut(s) 337, 469, 1069, 1077
BtsIMutI CAGTG 3 cut(s) 85, 333, 438
Cac8I GCNNGC 3 cut(s) 784, 893, 959
CaiI CAGNNNCTG 3 cut(s) 260, 1021, 1046
Cfr10I RCCGGY 2 cut(s) 148, 782
Cfr13I GGNCC 4 cut(s) 151, 820, 869, 970
Csp6I GTAC 2 cut(s) 456, 495
CviAII CATG 8 cut(s) 219, 343, 572, 662, 828, 836, 1147, 1159
CviQI GTAC 2 cut(s) 456, 495
DdeI CTNAG 7 cut(s) 140, 635, 757, 805, 1014, 1032, 1059
DpnI GATC 6 cut(s) 84, 136, 213, 803, 941, 978
DpnII GATC 6 cut(s) 82, 134, 211, 801, 939, 976
DraIII CACNNNGTG 1 cut(s) 1103
DrdI GACNNNNNNGTC 1 cut(s) 719
DseDI GACNNNNNNGTC 1 cut(s) 719
EaeI YGGCCR 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 69, 112
Eco47I GGWCC 2 cut(s) 869, 970
Eco57I CTGAAG 1 cut(s) 871
EcoO109I RGGNCCY 1 cut(s) 820
EcoRII CCWGG 1 cut(s) 859
EcoT14I CCWWGG 2 cut(s) 69, 112
EcoT22I ATGCAT 1 cut(s) 523
ErhI CCWWGG 2 cut(s) 69, 112
FaeI CATG 8 cut(s) 222, 346, 575, 665, 831, 839, 1150, 1162
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FatI CATG 8 cut(s) 218, 342, 571, 661, 827, 835, 1146, 1158
FauNDI CATATG 1 cut(s) 618
FblI GTMKAC 1 cut(s) 1037
Fnu4HI GCNGC 5 cut(s) 223, 226, 840, 1125, 1137
FokI GGATG 4 cut(s) 344, 476, 1076, 1084
Fsp4HI GCNGC 5 cut(s) 223, 226, 840, 1125, 1137
FspBI CTAG 4 cut(s) 182, 396, 686, 1101
GluI GCNGC 5 cut(s) 223, 226, 840, 1125, 1137
GsaI CCCAGC 1 cut(s) 1050
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 8 cut(s) 5, 92, 152, 488, 623, 726, 782, 822
HapII CCGG 2 cut(s) 149, 783
Hin1II CATG 8 cut(s) 222, 346, 575, 665, 831, 839, 1150, 1162
HincII GTYRAC 2 cut(s) 267, 1086
HindII GTYRAC 2 cut(s) 267, 1086
HindIII AAGCTT 2 cut(s) 142, 508
HinfI GANTC 2 cut(s) 428, 1094
HpaII CCGG 2 cut(s) 149, 783
HphI GGTGA 2 cut(s) 57, 1089
Hpy166II GTNNAC 7 cut(s) 76, 267, 319, 438, 1038, 1086, 1153
Hpy188I TCNGA 5 cut(s) 423, 760, 808, 1023, 1060
Hpy188III TCNNGA 2 cut(s) 86, 630
Hpy8I GTNNAC 7 cut(s) 76, 267, 319, 438, 1038, 1086, 1153
Hpy99I CGWCG 1 cut(s) 800
HpyAV CCTTC 6 cut(s) 44, 284, 578, 676, 772, 1195
HpyCH4III ACNGT 4 cut(s) 80, 328, 475, 551
HpyCH4V TGCA 7 cut(s) 76, 222, 306, 521, 607, 839, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 123, 828, 1133
HpyF3I CTNAG 7 cut(s) 140, 635, 757, 805, 1014, 1032, 1059
Hsp92II CATG 8 cut(s) 222, 346, 575, 665, 831, 839, 1150, 1162
KpnI GGTACC 1 cut(s) 459
KroI GCCGGC 1 cut(s) 782
KroNI GCCGGC 1 cut(s) 784
Kzo9I GATC 6 cut(s) 82, 134, 211, 801, 939, 976
LmnI GCTCC 2 cut(s) 1043, 1107
Lsp1109I GCAGC 5 cut(s) 234, 237, 851, 1111, 1123
LweI GCATC 3 cut(s) 126, 266, 530
MaeI CTAG 4 cut(s) 182, 396, 686, 1101
MaeIII GTNAC 2 cut(s) 290, 908
MalI GATC 6 cut(s) 84, 136, 213, 803, 941, 978
MboI GATC 6 cut(s) 82, 134, 211, 801, 939, 976
MboII GAAGA 7 cut(s) 19, 22, 110, 275, 672, 716, 719
MfeI CAATTG 1 cut(s) 1140
MflI RGATCY 2 cut(s) 801, 976
MhlI GDGCHC 2 cut(s) 78, 963
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 5 cut(s) 733, 991, 1007, 1027, 1140
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 1088
MmeI TCCRAC 1 cut(s) 427
Mox20I TGGCCA 1 cut(s) 488
Mph1103I ATGCAT 1 cut(s) 523
MroNI GCCGGC 1 cut(s) 782
MscI TGGCCA 1 cut(s) 488
MseI TTAA 3 cut(s) 300, 855, 987
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 842
MspI CCGG 2 cut(s) 149, 783
MspR9I CCNGG 1 cut(s) 861
MunI CAATTG 1 cut(s) 1140
MvaI CCWGG 1 cut(s) 861
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 3 cut(s) 123, 828, 1133
NaeI GCCGGC 1 cut(s) 784
NdeI CATATG 1 cut(s) 618
NdeII GATC 6 cut(s) 82, 134, 211, 801, 939, 976
NgoMIV GCCGGC 1 cut(s) 782
NlaIII CATG 8 cut(s) 222, 346, 575, 665, 831, 839, 1150, 1162
NlaIV GGNNCC 3 cut(s) 241, 457, 972
NsiI ATGCAT 1 cut(s) 523
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 771
PdiI GCCGGC 1 cut(s) 784
PfeI GAWTC 1 cut(s) 428
PflMI CCANNNNNTGG 1 cut(s) 866
PkrI GCNGC 5 cut(s) 224, 227, 841, 1126, 1138
PleI GAGTC 1 cut(s) 1088
PpsI GAGTC 1 cut(s) 1088
Psp6I CCWGG 1 cut(s) 859
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 1 cut(s) 859
PspN4I GGNNCC 3 cut(s) 241, 457, 972
PspPI GGNCC 4 cut(s) 151, 820, 869, 970
PstNI CAGNNNCTG 3 cut(s) 260, 1021, 1046
PsuI RGATCY 2 cut(s) 801, 976
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 2 cut(s) 457, 496
RsaNI GTAC 2 cut(s) 456, 495
SaqAI TTAA 3 cut(s) 300, 855, 987
SatI GCNGC 5 cut(s) 223, 226, 840, 1125, 1137
Sau3AI GATC 6 cut(s) 82, 134, 211, 801, 939, 976
Sau96I GGNCC 4 cut(s) 151, 820, 869, 970
SchI GAGTC 1 cut(s) 1088
ScrFI CCNGG 1 cut(s) 861
SduI GDGCHC 2 cut(s) 78, 963
SfaNI GCATC 3 cut(s) 126, 266, 530
SfcI CTRYAG 2 cut(s) 127, 815
SinI GGWCC 2 cut(s) 869, 970
SpeI ACTAGT 1 cut(s) 395
Sse9I AATT 5 cut(s) 733, 991, 1007, 1027, 1140
SsiI CCGC 1 cut(s) 365
SspMI CTAG 4 cut(s) 182, 396, 686, 1101
StyD4I CCNGG 1 cut(s) 859
StyI CCWWGG 2 cut(s) 69, 112
TaaI ACNGT 4 cut(s) 80, 328, 475, 551
TaqI TCGA 2 cut(s) 431, 537
TasI AATT 5 cut(s) 733, 991, 1007, 1027, 1140
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 3 cut(s) 300, 855, 987
Tru9I TTAA 3 cut(s) 300, 855, 987
TscAI CASTG 3 cut(s) 85, 333, 445
TseI GCWGC 5 cut(s) 222, 225, 839, 1124, 1136
TspDTI ATGAA 7 cut(s) 31, 111, 543, 560, 650, 717, 1177
TspRI CASTG 3 cut(s) 85, 333, 445
Van91I CCANNNNNTGG 1 cut(s) 866
VneI GTGCAC 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 869, 970
XapI RAATTY 1 cut(s) 1007
XmiI GTMKAC 1 cut(s) 1037
XspI CTAG 4 cut(s) 182, 396, 686, 1101
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.