Rh3DG061600

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005399
Physical Location & Seq
Forward (+)
4134594 .. 4135639
1046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG061600.1

Sequence Viewer

Length: 588 bp
ATGGCCTTCTCAAAACTGAGCTTATTGTTTGCCTTTGTGCTTGTTGACGCCTTCCTCCTCCATTCTTCTCCAGTATATTGTAATGAAGAAGAAGACGATCTGTTCGAGGAACTCAACAGTTTCAGGGGATCCCTAAACCTTACAGCCTTCACAGAGAACGAAAAGGCCGATTGTATAGCCGACGAAATCTCGGATGAGCTAGAGGATGAGCTATGCTCTAAAGCAGCTGAATACCAGATTAAACCAGGAAATGGTCCTCAGTTCCCCTCCTTCACCAAGTCCTTAACCAAGTGTGACATAGACAACAGCACCACAACTGGTGGGATCATTTTGCCTGTTTGTGTGCGCAATTTGGACCCAGATCTGGTGCGTCAAAATTATACTGAAACCAAATTTGCTCAGTTTCTGAATGATTCCAAGTATACAGGAGCTGGGCTTGGTTCTAAGGATGATTGGATGATCGTTGTGTTGATCACTGACGCACATAGTGGGAGCTTTACTGGTGGTGCTGCTTCTTTGGTTGCAACTGGCATGGTTCACTACATGGTTGCTTTGTTGTTGGGATTGTTCTTGGTTTTTTCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

21.32

Weight (kDa)

4.31

Isoelectric Point (pI)

30.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 31 - 157 3.1e-50 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 251
AccI GTMKAC 1 cut(s) 422
AclWI GGATC 3 cut(s) 123, 136, 332
AcsI RAATTY 1 cut(s) 392
AcyI GRCGYC 1 cut(s) 48
AdeI CACNNNGTG 1 cut(s) 488
AfiI CCNNNNNNNGG 2 cut(s) 251, 364
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 6 cut(s) 21, 199, 211, 227, 431, 495
AluI AGCT 6 cut(s) 21, 199, 211, 227, 431, 495
AlwI GGATC 3 cut(s) 123, 136, 332
AlwNI CAGNNNCTG 2 cut(s) 406, 431
AoxI GGCC 2 cut(s) 3, 165
ApeKI GCWGC 2 cut(s) 224, 509
ApoI RAATTY 1 cut(s) 392
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 2 cut(s) 254, 355
AsuHPI GGTGA 1 cut(s) 265
AvaII GGWCC 2 cut(s) 254, 355
BamHI GGATCC 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 236, 496
BciT130I CCWGG 1 cut(s) 246
BclI TGATCA 1 cut(s) 471
BfaI CTAG 1 cut(s) 200
BglII AGATCT 1 cut(s) 361
BisI GCNGC 2 cut(s) 225, 510
BlsI GCNGC 2 cut(s) 226, 511
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 2 cut(s) 254, 355
BmgT120I GGNCC 2 cut(s) 254, 355
BmiI GGNNCC 2 cut(s) 130, 357
BmrFI CCNGG 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 1 cut(s) 54
BsaHI GRCGYC 1 cut(s) 48
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 364
Bse1I ACTGG 4 cut(s) 71, 322, 505, 532
BseBI CCWGG 1 cut(s) 246
BseGI GGATG 4 cut(s) 199, 211, 454, 462
BseLI CCNNNNNNNGG 2 cut(s) 251, 364
BseMII CTCAG 3 cut(s) 8, 272, 413
BseNI ACTGG 4 cut(s) 71, 322, 505, 532
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 2 cut(s) 236, 496
BseYI CCCAGC 1 cut(s) 431
BshFI GGCC 2 cut(s) 5, 167
BslI CCNNNNNNNGG 2 cut(s) 251, 364
BsnI GGCC 2 cut(s) 5, 167
Bsp143I GATC 6 cut(s) 97, 128, 324, 361, 459, 471
BspANI GGCC 2 cut(s) 5, 167
BspCNI CTCAG 3 cut(s) 9, 271, 412
BspLI GGNNCC 2 cut(s) 130, 357
BspPI GGATC 3 cut(s) 123, 136, 332
BsrI ACTGG 4 cut(s) 71, 322, 505, 532
BssMI GATC 6 cut(s) 97, 128, 324, 361, 459, 471
BssNAI GTATAC 1 cut(s) 423
BssNI GRCGYC 1 cut(s) 48
Bst1107I GTATAC 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 119
BstACI GRCGYC 1 cut(s) 48
BstDEI CTNAG 4 cut(s) 17, 258, 399, 444
BstF5I GGATG 4 cut(s) 199, 211, 454, 462
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 6 cut(s) 100, 131, 327, 364, 462, 474
BstMBI GATC 6 cut(s) 97, 128, 324, 361, 459, 471
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 236, 496
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 2 cut(s) 128, 361
BstYI RGATCY 2 cut(s) 128, 361
BstZ17I GTATAC 1 cut(s) 423
BsuRI GGCC 2 cut(s) 5, 167
BtsCI GGATG 4 cut(s) 199, 211, 454, 462
BtsIMutI CAGTG 1 cut(s) 474
CaiI CAGNNNCTG 2 cut(s) 406, 431
CfoI GCGC 1 cut(s) 348
Cfr13I GGNCC 2 cut(s) 254, 355
CseI GACGC 3 cut(s) 56, 359, 488
CviAII CATG 2 cut(s) 532, 544
DdeI CTNAG 4 cut(s) 17, 258, 399, 444
DpnI GATC 6 cut(s) 99, 130, 326, 363, 461, 473
DpnII GATC 6 cut(s) 97, 128, 324, 361, 459, 471
DraIII CACNNNGTG 1 cut(s) 488
Eco47I GGWCC 2 cut(s) 254, 355
EcoRII CCWGG 1 cut(s) 244
FaeI CATG 2 cut(s) 535, 547
FaiI YATR 9 cut(s) 76, 176, 214, 299, 381, 423, 486, 533, 545
FatI CATG 2 cut(s) 531, 543
FbaI TGATCA 1 cut(s) 471
FblI GTMKAC 1 cut(s) 422
Fnu4HI GCNGC 2 cut(s) 225, 510
FokI GGATG 4 cut(s) 206, 218, 461, 469
Fsp4HI GCNGC 2 cut(s) 225, 510
FspBI CTAG 1 cut(s) 200
FspI TGCGCA 1 cut(s) 347
GlaI GCGC 1 cut(s) 347
GluI GCNGC 2 cut(s) 225, 510
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 54
HaeIII GGCC 2 cut(s) 5, 167
HgaI GACGC 3 cut(s) 56, 359, 488
HhaI GCGC 1 cut(s) 348
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 2 cut(s) 535, 547
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 1 cut(s) 413
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 3 cut(s) 46, 423, 538
Hpy188I TCNGA 2 cut(s) 193, 408
Hpy8I GTNNAC 3 cut(s) 46, 423, 538
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 4 cut(s) 16, 61, 157, 280
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4V TGCA 1 cut(s) 524
HpyF3I CTNAG 4 cut(s) 17, 258, 399, 444
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 2 cut(s) 535, 547
HspAI GCGC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 471
Kzo9I GATC 6 cut(s) 97, 128, 324, 361, 459, 471
LmnI GCTCC 2 cut(s) 428, 492
Lsp1109I GCAGC 2 cut(s) 236, 496
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 293
MalI GATC 6 cut(s) 99, 130, 326, 363, 461, 473
MboI GATC 6 cut(s) 97, 128, 324, 361, 459, 471
MboII GAAGA 4 cut(s) 57, 98, 101, 104
MflI RGATCY 2 cut(s) 128, 361
MluCI AATT 3 cut(s) 349, 376, 392
MnlI CCTC 6 cut(s) 65, 68, 100, 196, 267, 277
MseI TTAA 2 cut(s) 240, 284
MspA1I CMGCKG 1 cut(s) 227
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
NdeII GATC 6 cut(s) 97, 128, 324, 361, 459, 471
NlaIII CATG 2 cut(s) 535, 547
NlaIV GGNNCC 2 cut(s) 130, 357
NmuCI GTSAC 1 cut(s) 293
NsbI TGCGCA 1 cut(s) 347
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 165
PfeI GAWTC 1 cut(s) 413
PflMI CCANNNNNTGG 1 cut(s) 251
PkrI GCNGC 2 cut(s) 226, 511
Psp6I CCWGG 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 130, 357
PspPI GGNCC 2 cut(s) 254, 355
PstNI CAGNNNCTG 2 cut(s) 406, 431
PsuI RGATCY 2 cut(s) 128, 361
PvuII CAGCTG 1 cut(s) 227
SaqAI TTAA 2 cut(s) 240, 284
SatI GCNGC 2 cut(s) 225, 510
Sau3AI GATC 6 cut(s) 97, 128, 324, 361, 459, 471
Sau96I GGNCC 2 cut(s) 254, 355
ScrFI CCNGG 1 cut(s) 246
SetI ASST 7 cut(s) 23, 141, 201, 213, 229, 433, 497
SinI GGWCC 2 cut(s) 254, 355
Sse9I AATT 3 cut(s) 349, 376, 392
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 244
TaaI ACNGT 1 cut(s) 119
TaqI TCGA 1 cut(s) 105
TasI AATT 3 cut(s) 349, 376, 392
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 2 cut(s) 240, 284
Tru9I TTAA 2 cut(s) 240, 284
TscAI CASTG 1 cut(s) 481
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 2 cut(s) 224, 509
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 99
TspRI CASTG 1 cut(s) 481
Van91I CCANNNNNTGG 1 cut(s) 251
VpaK11BI GGWCC 2 cut(s) 254, 355
XapI RAATTY 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 422
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.