MD12G1220500.v1.1

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005399
Physical Location & Seq
Reverse (-)
29728002 .. 29728598
597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1220500.v1.1.491

Sequence Viewer

Length: 597 bp
ATGGTTGTAGCATTTGGTATTATTTTAGAATTTCAGTGGCTAATTAACCATTTTCAAACTTACCATACCTATTTGCCTGCAGATAAGGATGAGGATACCCTTCTCCAAGGCATAAACAGTTACAGAACCTCACTAAACCTGCCGGCCCTCGTCAAGAACGGTAACGCAGGTTGCCTAGCAGATGAAATTGCTGATGACATGGAAGACCAGCCTTGCAGCAGCCCCACCAATGGAGCCAACATTCTGGCAACCTCGCAGACTCCGCTCGCCAATCTTCCCAAGCACTTGGGAAAATGCAAAATCGATGCGAACAGCGCATCAGATGGAGTTATTCTCCCTGTTTGTGTGCCTAAGTTGGTCCCAACTCTGGTACTTACCAATTATACTCACCAACCGCAATTCGCAAAGTATCTGAACGATACGAAGTTCACTGGAGTTGGACTTGGATCCGAGGATGATTGGATGGTGGTGGTTTTGGCCTCAAATACCCCAACTGGAAGCCTTGCTAATGCAGCAAGCTCTTTAGTTTCAGCCGTTGGTTTTGGTCATTTCTTGGTCTCCATTTTGGTAGGGTTGTGTGTTATTTTGGTCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

21.07

Weight (kDa)

4.81

Isoelectric Point (pI)

27.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 31 - 159 5.7e-53 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 147, 158
AccBSI CCGCTC 1 cut(s) 265
AciI CCGC 2 cut(s) 263, 395
AclWI GGATC 2 cut(s) 441, 454
AcsI RAATTY 1 cut(s) 29
AfaI GTAC 1 cut(s) 372
AfiI CCNNNNNNNGG 2 cut(s) 230, 367
AgsI TTSAA 1 cut(s) 56
AluBI AGCT 2 cut(s) 519, 594
AluI AGCT 2 cut(s) 519, 594
Alw26I GTCTC 1 cut(s) 562
AlwI GGATC 2 cut(s) 441, 454
AoxI GGCC 2 cut(s) 144, 477
ApeKI GCWGC 3 cut(s) 216, 219, 512
ApoI RAATTY 1 cut(s) 29
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 2 cut(s) 145, 358
AsuHPI GGTGA 1 cut(s) 380
AvaII GGWCC 1 cut(s) 358
BamHI GGATCC 1 cut(s) 446
BbsI GAAGAC 1 cut(s) 210
BbvI GCAGC 3 cut(s) 228, 231, 524
BccI CCATC 2 cut(s) 317, 457
BceAI ACGGC 1 cut(s) 518
BciVI GTATCC 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 562
BfaI CTAG 1 cut(s) 176
BfmI CTRYAG 1 cut(s) 78
BfuAI ACCTGC 2 cut(s) 147, 158
BfuI GTATCC 1 cut(s) 88
BisI GCNGC 3 cut(s) 217, 220, 513
BlsI GCNGC 3 cut(s) 218, 221, 514
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 145, 358
BmiI GGNNCC 3 cut(s) 235, 360, 448
BmsI GCATC 2 cut(s) 295, 326
BpiI GAAGAC 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 318, 350
BpmI CTGGAG 1 cut(s) 453
Bsa29I ATCGAT 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 562
BsaJI CCNNGG 2 cut(s) 106, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 367
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 436, 499
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 106, 450
BseGI GGATG 3 cut(s) 94, 460, 468
BseLI CCNNNNNNNGG 2 cut(s) 230, 367
BseNI ACTGG 2 cut(s) 436, 499
BseXI GCAGC 3 cut(s) 228, 231, 524
BshFI GGCC 2 cut(s) 146, 479
BshVI ATCGAT 1 cut(s) 303
BsiSI CCGG 1 cut(s) 143
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 2 cut(s) 230, 367
BsmAI GTCTC 1 cut(s) 562
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 2 cut(s) 146, 479
Bso31I GGTCTC 1 cut(s) 562
Bsp143I GATC 1 cut(s) 446
BspACI CCGC 2 cut(s) 263, 395
BspANI GGCC 2 cut(s) 146, 479
BspDI ATCGAT 1 cut(s) 303
BspLI GGNNCC 3 cut(s) 235, 360, 448
BspMAI CTGCAG 1 cut(s) 82
BspMI ACCTGC 2 cut(s) 147, 158
BspPI GGATC 2 cut(s) 441, 454
BspTNI GGTCTC 1 cut(s) 562
BsrBI CCGCTC 1 cut(s) 265
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 2 cut(s) 436, 499
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 2 cut(s) 106, 450
BssMI GATC 1 cut(s) 446
BssT1I CCWWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 119, 161
BstC8I GCNNGC 4 cut(s) 78, 144, 267, 517
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 3 cut(s) 94, 460, 468
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 1 cut(s) 449
BstMAI GTCTC 1 cut(s) 562
BstMBI GATC 1 cut(s) 446
BstMWI GCNNNNNNNGC 3 cut(s) 262, 314, 512
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 3 cut(s) 228, 231, 524
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 446
BstXI CCANNNNNNTGG 2 cut(s) 244, 286
BstYI RGATCY 1 cut(s) 446
Bsu15I ATCGAT 1 cut(s) 303
BsuI GTATCC 1 cut(s) 88
BsuRI GGCC 2 cut(s) 146, 479
BsuTUI ATCGAT 1 cut(s) 303
BtsCI GGATG 3 cut(s) 94, 460, 468
BtsIMutI CAGTG 2 cut(s) 41, 429
BveI ACCTGC 2 cut(s) 147, 158
Cac8I GCNNGC 4 cut(s) 78, 144, 267, 517
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 2 cut(s) 145, 358
ClaI ATCGAT 1 cut(s) 303
Csp6I GTAC 1 cut(s) 371
CviAII CATG 1 cut(s) 199
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 1 cut(s) 351
DpnI GATC 1 cut(s) 448
DpnII GATC 1 cut(s) 446
Eco130I CCWWGG 1 cut(s) 106
Eco31I GGTCTC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 1 cut(s) 202
FaiI YATR 4 cut(s) 66, 113, 200, 384
FaqI GGGAC 1 cut(s) 344
FatI CATG 1 cut(s) 198
Fnu4HI GCNGC 3 cut(s) 217, 220, 513
FokI GGATG 3 cut(s) 101, 467, 475
Fsp4HI GCNGC 3 cut(s) 217, 220, 513
FspBI CTAG 1 cut(s) 176
GlaI GCGC 1 cut(s) 316
GluI GCNGC 3 cut(s) 217, 220, 513
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 2 cut(s) 146, 479
HapII CCGG 1 cut(s) 143
HhaI GCGC 1 cut(s) 317
Hin1II CATG 1 cut(s) 202
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HinfI GANTC 1 cut(s) 259
HpaII CCGG 1 cut(s) 143
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 1 cut(s) 429
Hpy188I TCNGA 3 cut(s) 322, 414, 451
Hpy188III TCNNGA 1 cut(s) 154
Hpy8I GTNNAC 1 cut(s) 429
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 2 cut(s) 119, 161
HpyCH4V TGCA 4 cut(s) 80, 216, 297, 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 262, 314, 512
HpyF3I CTNAG 1 cut(s) 351
Hsp92II CATG 1 cut(s) 202
HspAI GCGC 1 cut(s) 315
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Kzo9I GATC 1 cut(s) 446
LmnI GCTCC 1 cut(s) 233
Lsp1109I GCAGC 3 cut(s) 228, 231, 524
LweI GCATC 2 cut(s) 295, 326
MaeI CTAG 1 cut(s) 176
MaeIII GTNAC 2 cut(s) 119, 161
MalI GATC 1 cut(s) 448
MbiI CCGCTC 1 cut(s) 265
MboI GATC 1 cut(s) 446
MboII GAAGA 2 cut(s) 215, 266
MflI RGATCY 1 cut(s) 446
MluCI AATT 5 cut(s) 29, 42, 186, 379, 398
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 6 cut(s) 85, 139, 158, 262, 445, 490
MroNI GCCGGC 1 cut(s) 142
MseI TTAA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 594
MspI CCGG 1 cut(s) 143
MwoI GCNNNNNNNGC 3 cut(s) 262, 314, 512
NaeI GCCGGC 1 cut(s) 144
NdeII GATC 1 cut(s) 446
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 3 cut(s) 235, 360, 448
PdiI GCCGGC 1 cut(s) 144
PkrI GCNGC 3 cut(s) 218, 221, 514
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PspN4I GGNNCC 3 cut(s) 235, 360, 448
PspPI GGNCC 2 cut(s) 145, 358
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 594
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 3 cut(s) 217, 220, 513
Sau3AI GATC 1 cut(s) 446
Sau96I GGNCC 2 cut(s) 145, 358
SchI GAGTC 1 cut(s) 253
SetI ASST 7 cut(s) 71, 131, 141, 172, 254, 521, 596
SfaNI GCATC 2 cut(s) 295, 326
SfcI CTRYAG 1 cut(s) 78
SinI GGWCC 1 cut(s) 358
Sse9I AATT 5 cut(s) 29, 42, 186, 379, 398
SsiI CCGC 2 cut(s) 263, 395
SspMI CTAG 1 cut(s) 176
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 2 cut(s) 119, 161
TaqI TCGA 1 cut(s) 303
TasI AATT 5 cut(s) 29, 42, 186, 379, 398
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 2 cut(s) 41, 436
TseI GCWGC 3 cut(s) 216, 219, 512
TspDTI ATGAA 1 cut(s) 198
TspRI CASTG 2 cut(s) 41, 436
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 29
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.