AT1G56430

nicotianamine synthase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008309
Physical Location & Seq
Forward (+)
21136835 .. 21138277
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G56430.1

Sequence Viewer

Length: 975 bp
ATGGGTTATTGCCAAGACGACCAACTCGTAAACAAGATCTGCGATCTTTACGAAAAGATCTCGAAGCTTGAGACCCTAAAGCCTTGTGAAGATGTCGACACTCTCTTCAAGCAGCTCGTGTCCACATGCATACCACCAAACCCTAACATCGACGTCACCAAGATGTCTGAAAACATCCAAGAGATGAGATCAAACCTCATCAAAATCTGTGGTGAGGCTGAAGGTTACTTAGAGCATCACTTCTCTTCAATCTTAACCTCTTTTGAAGATAACCCCCTTCATCATTTGAATCTTTTTCCTTACTACAACAACTACCTCAAACTAAGCAAGCTCGAGTTTGATCTCCTCGAACAGAACCTAAACGGTTTTGTTCCAAGGACTGTAGCTTTCATTGGCTCTGGTCCTCTCCCTCTTACTTCCGTCGTTCTTGCCTCTTCCCATCTCAAAGACTCGATCTTTCATAACTTTGACATCGACCCATCAGCGAATATGGTAGCAGCTCGTTTGGTTTCGTCTGATCCTGATCTTTCTCAACGTATGTTTTTCCATACTGTTGATATAATGGATGTAACCGAGAGCTTGAAGGGCTTCGACGTTGTGTTCTTGGCTGCTCTTGTAGGGATGGATAAAAAGGAGAAGGTTAAGGTGGTCGAGCATCTTGAGAAACACATGTCTCCTGGTGCTTTGCTCATGCTGAGAAGCGCTCATGGACCTAGAGCTTTTCTCTATCCAATCGTTGAGCCTTGTGATCTCGAAGGTTTCGAAGTTTTATCGGTTTATCACCCTACCGATGAAGTTATCAACTCCATTGTAATCTCAAGGAAGCTAGGTGAAGATGCTAATGGTGTTGTTCATGATCATATAGATCAAGCTTCGGATCTCGCGTGTAACTGTTCCAAGATCCACGTGATTATGAACAAGAAGAAGAGCATTATCGAGGAGTTCGCAGGTGCTAATGAAGAACAACTTACCTAG

Protein Analysis

324

Amino Acids

36.35

Weight (kDa)

5.08

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 7 - 277 1.7e-129 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 938
AatII GACGTC 1 cut(s) 156
Acc36I ACCTGC 1 cut(s) 938
AccI GTMKAC 1 cut(s) 96
AccII CGCG 1 cut(s) 884
AclWI GGATC 3 cut(s) 512, 885, 895
AcuI CTGAAG 1 cut(s) 240
AcvI CACGTG 1 cut(s) 907
AcyI GRCGYC 1 cut(s) 153
AfeI AGCGCT 1 cut(s) 703
AflIII ACRYGT 1 cut(s) 669
AgsI TTSAA 5 cut(s) 109, 249, 266, 289, 583
AjnI CCWGG 1 cut(s) 676
AluBI AGCT 9 cut(s) 67, 115, 331, 386, 500, 579, 719, 826, 872
AluI AGCT 9 cut(s) 67, 115, 331, 386, 500, 579, 719, 826, 872
Alw26I GTCTC 2 cut(s) 65, 678
AlwI GGATC 3 cut(s) 512, 885, 895
Ama87I CYCGRG 1 cut(s) 332
Aor51HI AGCGCT 1 cut(s) 703
ApeKI GCWGC 3 cut(s) 112, 497, 608
Asp700I GAANNNNTTC 1 cut(s) 587
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 2 cut(s) 401, 710
AsuHPI GGTGA 4 cut(s) 148, 224, 773, 842
AsuII TTCGAA 1 cut(s) 762
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 2 cut(s) 401, 710
BauI CACGAG 1 cut(s) 116
BbrPI CACGTG 1 cut(s) 907
BbvI GCAGC 3 cut(s) 124, 509, 595
BccI CCATC 3 cut(s) 447, 487, 616
BciT130I CCWGG 1 cut(s) 678
BclI TGATCA 1 cut(s) 856
BcoDI GTCTC 2 cut(s) 65, 678
BfaI CTAG 3 cut(s) 714, 827, 973
BfmI CTRYAG 1 cut(s) 381
BfoI RGCGCY 1 cut(s) 705
BfuAI ACCTGC 1 cut(s) 938
BglII AGATCT 2 cut(s) 36, 57
BisI GCNGC 3 cut(s) 113, 498, 609
BlsI GCNGC 3 cut(s) 114, 499, 610
Bme1390I CCNGG 1 cut(s) 678
Bme18I GGWCC 2 cut(s) 401, 710
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 401, 710
BmrFI CCNGG 1 cut(s) 678
BmsI GCATC 3 cut(s) 244, 664, 826
BplI GAGNNNNNCTC 4 cut(s) 688, 720, 708, 740
Bpu14I TTCGAA 1 cut(s) 762
BpuEI CTTGAG 3 cut(s) 89, 680, 802
BsaAI YACGTR 1 cut(s) 907
BsaBI GATNNNNATC 1 cut(s) 522
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 374
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 1 cut(s) 678
BseDI CCNNGG 1 cut(s) 374
BseGI GGATG 3 cut(s) 174, 571, 627
BseJI GATNNNNATC 1 cut(s) 522
BseMII CTCAG 1 cut(s) 686
BseRI GAGGAG 2 cut(s) 335, 953
BseXI GCAGC 3 cut(s) 124, 509, 595
Bsh1236I CGCG 1 cut(s) 884
BsiHKCI CYCGRG 1 cut(s) 332
BsmAI GTCTC 2 cut(s) 65, 678
Bso31I GGTCTC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 332
Bsp119I TTCGAA 1 cut(s) 762
BspCNI CTCAG 1 cut(s) 687
BspFNI CGCG 1 cut(s) 884
BspHI TCATGA 1 cut(s) 853
BspMI ACCTGC 1 cut(s) 938
BspPI GGATC 3 cut(s) 512, 885, 895
BspQI GCTCTTC 1 cut(s) 920
BspT104I TTCGAA 1 cut(s) 762
BspTNI GGTCTC 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 374
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 374
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 678
Bst4CI ACNGT 4 cut(s) 365, 382, 553, 893
Bst6I CTCTTC 4 cut(s) 110, 250, 439, 920
BstACI GRCGYC 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 907
BstBI TTCGAA 1 cut(s) 762
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 3 cut(s) 229, 323, 695
BstF5I GGATG 3 cut(s) 174, 571, 627
BstFNI CGCG 1 cut(s) 884
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 1 cut(s) 704
BstMAI GTCTC 2 cut(s) 65, 678
BstMWI GCNNNNNNNGC 1 cut(s) 585
BstNI CCWGG 1 cut(s) 678
BstNSI RCATGY 2 cut(s) 129, 673
BstSCI CCNGG 1 cut(s) 676
BstSFI CTRYAG 1 cut(s) 381
BstUI CGCG 1 cut(s) 884
BstV1I GCAGC 3 cut(s) 124, 509, 595
BstX2I RGATCY 4 cut(s) 36, 57, 877, 900
BstYI RGATCY 4 cut(s) 36, 57, 877, 900
BtsCI GGATG 3 cut(s) 174, 571, 627
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 1 cut(s) 329
CciI TCATGA 1 cut(s) 853
CfoI GCGC 1 cut(s) 704
Cfr13I GGNCC 2 cut(s) 401, 710
CspCI CAANNNNNGTGG 2 cut(s) 190, 225
CviAII CATG 5 cut(s) 126, 670, 691, 707, 854
DdeI CTNAG 3 cut(s) 229, 323, 695
Eam1104I CTCTTC 4 cut(s) 110, 250, 439, 920
EarI CTCTTC 4 cut(s) 110, 250, 439, 920
Eco130I CCWWGG 1 cut(s) 374
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 2 cut(s) 401, 710
Eco47III AGCGCT 1 cut(s) 703
Eco57I CTGAAG 1 cut(s) 240
Eco72I CACGTG 1 cut(s) 907
Eco88I CYCGRG 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 676
EcoT14I CCWWGG 1 cut(s) 374
EcoT22I ATGCAT 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 374
FaeI CATG 5 cut(s) 129, 673, 694, 710, 857
FalI AAGNNNNNCTT 1 cut(s) 951
FatI CATG 5 cut(s) 125, 669, 690, 706, 853
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 3 cut(s) 113, 498, 609
FokI GGATG 3 cut(s) 161, 578, 634
Fsp4HI GCNGC 3 cut(s) 113, 498, 609
FspBI CTAG 3 cut(s) 714, 827, 973
GlaI GCGC 1 cut(s) 703
GluI GCNGC 3 cut(s) 113, 498, 609
HaeII RGCGCY 1 cut(s) 705
HhaI GCGC 1 cut(s) 704
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 5 cut(s) 129, 673, 694, 710, 857
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 2 cut(s) 65, 870
HinfI GANTC 2 cut(s) 289, 449
HphI GGTGA 4 cut(s) 148, 224, 773, 842
Hpy166II GTNNAC 3 cut(s) 31, 97, 123
Hpy188I TCNGA 3 cut(s) 169, 517, 877
Hpy188III TCNNGA 5 cut(s) 61, 521, 659, 752, 854
Hpy8I GTNNAC 3 cut(s) 31, 97, 123
Hpy99I CGWCG 3 cut(s) 155, 425, 596
HpyAV CCTTC 5 cut(s) 215, 287, 577, 631, 749
HpyCH4III ACNGT 4 cut(s) 365, 382, 553, 893
HpyCH4IV ACGT 4 cut(s) 153, 535, 594, 906
HpyCH4V TGCA 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 1 cut(s) 585
HpyF3I CTNAG 3 cut(s) 229, 323, 695
HpySE526I ACGT 4 cut(s) 153, 535, 594, 906
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 5 cut(s) 129, 673, 694, 710, 857
HspAI GCGC 1 cut(s) 702
Ksp22I TGATCA 1 cut(s) 856
LguI GCTCTTC 1 cut(s) 920
LpnPI CCDG 5 cut(s) 384, 534, 663, 690, 933
Lsp1109I GCAGC 3 cut(s) 124, 509, 595
LweI GCATC 3 cut(s) 244, 664, 826
MaeI CTAG 3 cut(s) 714, 827, 973
MaeII ACGT 4 cut(s) 153, 535, 594, 906
MaeIII GTNAC 4 cut(s) 154, 224, 568, 887
MboII GAAGA 9 cut(s) 97, 101, 237, 278, 426, 845, 934, 937, 971
MflI RGATCY 4 cut(s) 36, 57, 877, 900
MlyI GAGTC 1 cut(s) 443
MnlI CCTC 9 cut(s) 206, 208, 268, 326, 356, 414, 420, 442, 931
Mph1103I ATGCAT 1 cut(s) 131
MroXI GAANNNNTTC 1 cut(s) 587
MseI TTAA 2 cut(s) 254, 642
MslI CAYNNNNRTG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 678
MvaI CCWGG 1 cut(s) 678
MvnI CGCG 1 cut(s) 884
MwoI GCNNNNNNNGC 1 cut(s) 585
NlaIII CATG 5 cut(s) 129, 673, 694, 710, 857
NmuCI GTSAC 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 131
NspI RCATGY 2 cut(s) 129, 673
NspV TTCGAA 1 cut(s) 762
PaeR7I CTCGAG 1 cut(s) 332
PagI TCATGA 1 cut(s) 853
PaqCI CACCTGC 1 cut(s) 938
PciI ACATGT 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 920
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 759, 881
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 1 cut(s) 289
PkrI GCNGC 3 cut(s) 114, 499, 610
PleI GAGTC 1 cut(s) 443
PmaCI CACGTG 1 cut(s) 907
PmlI CACGTG 1 cut(s) 907
PpsI GAGTC 1 cut(s) 443
Ppu21I YACGTR 1 cut(s) 907
PscI ACATGT 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 676
PspCI CACGTG 1 cut(s) 907
PspGI CCWGG 1 cut(s) 676
PspPI GGNCC 2 cut(s) 401, 710
PspXI VCTCGAGB 1 cut(s) 332
PsuI RGATCY 4 cut(s) 36, 57, 877, 900
RseI CAYNNNNRTG 1 cut(s) 161
SalI GTCGAC 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 920
SaqAI TTAA 2 cut(s) 254, 642
SatI GCNGC 3 cut(s) 113, 498, 609
Sau96I GGNCC 2 cut(s) 401, 710
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 1 cut(s) 678
SfaNI GCATC 3 cut(s) 244, 664, 826
SfcI CTRYAG 1 cut(s) 381
Sfr274I CTCGAG 1 cut(s) 332
SfuI TTCGAA 1 cut(s) 762
SinI GGWCC 2 cut(s) 401, 710
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 4 cut(s) 68, 332, 659, 817
SmoI CTYRAG 4 cut(s) 68, 332, 659, 817
SspMI CTAG 3 cut(s) 714, 827, 973
StyD4I CCNGG 1 cut(s) 676
StyI CCWWGG 1 cut(s) 374
TaaI ACNGT 4 cut(s) 365, 382, 553, 893
TaiI ACGT 4 cut(s) 156, 538, 597, 909
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 2 cut(s) 254, 642
Tru9I TTAA 2 cut(s) 254, 642
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 3 cut(s) 112, 497, 608
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 7 cut(s) 269, 379, 449, 807, 842, 929, 972
TspGWI ACGGA 1 cut(s) 409
VpaK11BI GGWCC 2 cut(s) 401, 710
XceI RCATGY 2 cut(s) 129, 673
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 3 cut(s) 714, 827, 973
ZraI GACGTC 1 cut(s) 154
Zsp2I ATGCAT 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.