MD13G1268200.v1.1

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008309
Physical Location & Seq
Forward (+)
35219036 .. 35219347
312 bp
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UTR
Exon/CDS
Intron
MD13G1268200.v1.1.491

Sequence Viewer

Length: 312 bp
ATGCCTCTTACTTCCTTCATATTGGCCACTTATCACATGAAATCCACTCATTTCGACAACTTTGATATCGATGAGAAGGCAAATGATGTGGCTCAAAAGATTGTTTTTACAGACGTTGAGCTATCGAAGAGGATGAAGTTTGAGACAAATGATATTATGAAAGTTAGAGAGAAGCTTGCAGAATATGAGTGCATATTTTTGGCAGCTTTGGTGGGGATGAATAAGGAAAATAAGGTAAAGATTCTTCAGCATATTAGGAAGTATATGAAGGATGGAAGCGTTTTGCTTGTGAGGAGCGCCAAAGGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

104

Amino Acids

11.9

Weight (kDa)

9.01

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 1 - 103 2.6e-38 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 230
AluBI AGCT 3 cut(s) 121, 175, 206
AluI AGCT 3 cut(s) 121, 175, 206
Alw26I GTCTC 1 cut(s) 137
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 1 cut(s) 203
AspLEI GCGC 1 cut(s) 299
BalI TGGCCA 1 cut(s) 26
BarI GAAGNNNNNNTAC 2 cut(s) 228, 260
BbvI GCAGC 1 cut(s) 215
BccI CCATC 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 137
BfoI RGCGCY 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
Bsa29I ATCGAT 1 cut(s) 69
BseCI ATCGAT 1 cut(s) 69
BseGI GGATG 3 cut(s) 138, 222, 277
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 215
BshFI GGCC 1 cut(s) 26
BshVI ATCGAT 1 cut(s) 69
BsmAI GTCTC 1 cut(s) 137
BsnI GGCC 1 cut(s) 26
BspANI GGCC 1 cut(s) 26
BspDI ATCGAT 1 cut(s) 69
Bst6I CTCTTC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 177
BstF5I GGATG 3 cut(s) 138, 222, 277
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstMAI GTCTC 1 cut(s) 137
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 215
Bsu15I ATCGAT 1 cut(s) 69
BsuRI GGCC 1 cut(s) 26
BsuTUI ATCGAT 1 cut(s) 69
BtsCI GGATG 3 cut(s) 138, 222, 277
Cac8I GCNNGC 1 cut(s) 177
CfoI GCGC 1 cut(s) 299
ClaI ATCGAT 1 cut(s) 69
CspCI CAANNNNNGTGG 2 cut(s) 69, 104
CviAII CATG 1 cut(s) 37
CviJI RGCY 6 cut(s) 26, 92, 121, 175, 206, 308
CviKI_1 RGCY 6 cut(s) 26, 92, 121, 175, 206, 308
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco32I GATATC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 230
EcoRV GATATC 1 cut(s) 67
FaeI CATG 1 cut(s) 40
FaiI YATR 8 cut(s) 20, 38, 158, 186, 194, 252, 264, 266
FatI CATG 1 cut(s) 36
Fnu4HI GCNGC 1 cut(s) 204
FokI GGATG 3 cut(s) 145, 229, 284
Fsp4HI GCNGC 1 cut(s) 204
GlaI GCGC 1 cut(s) 298
GluI GCNGC 1 cut(s) 204
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 1 cut(s) 26
HhaI GCGC 1 cut(s) 299
Hin1II CATG 1 cut(s) 40
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 1 cut(s) 241
HpyAV CCTTC 3 cut(s) 25, 70, 262
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 2 cut(s) 179, 192
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 1 cut(s) 40
HspAI GCGC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 1 cut(s) 215
MaeII ACGT 1 cut(s) 114
MboII GAAGA 2 cut(s) 139, 236
MlsI TGGCCA 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 26
MnlI CCTC 3 cut(s) 15, 123, 285
Mox20I TGGCCA 1 cut(s) 26
MscI TGGCCA 1 cut(s) 26
Msp20I TGGCCA 1 cut(s) 26
MwoI GCNNNNNNNGC 1 cut(s) 305
NlaIII CATG 1 cut(s) 40
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 1 cut(s) 205
SatI GCNGC 1 cut(s) 204
SetI ASST 5 cut(s) 117, 123, 177, 208, 237
SgeI CNNG 3 cut(s) 49, 188, 299
TaiI ACGT 1 cut(s) 117
TaqI TCGA 3 cut(s) 54, 69, 125
TfiI GAWTC 1 cut(s) 241
TseI GCWGC 1 cut(s) 203
TspDTI ATGAA 6 cut(s) 7, 53, 149, 173, 233, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.