MD13G1268300.v1.1

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008309
Physical Location & Seq
Forward (+)
35228312 .. 35228479
168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1268300.v1.1.491

Sequence Viewer

Length: 168 bp
ATGAAGGATGGAAGCGTTTTGCTAGTGAGGAGCGCTAAAGGGGCCAGAGCATTTTTGTACCCTGTTGTTGACGAGCATGACTTGCTGGTCTTTGAGCTTCTTACAATCTTCCATCCAAATAATGAGGTGATTAATTCCGTTGTTCTCGTGCGCAAGCCTATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

56

Amino Acids

6.22

Weight (kDa)

6.71

Isoelectric Point (pI)

14.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 1 - 53 4e-18 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
Acc16I TGCGCA 1 cut(s) 152
AfaI GTAC 1 cut(s) 59
AfeI AGCGCT 1 cut(s) 34
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
Aor51HI AGCGCT 1 cut(s) 34
AoxI GGCC 1 cut(s) 42
AseI ATTAAT 1 cut(s) 132
AspLEI GCGC 2 cut(s) 35, 153
AspS9I GGNCC 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 139
BauI CACGAG 1 cut(s) 146
BccI CCATC 2 cut(s) 2, 120
BfaI CTAG 2 cut(s) 23, 166
BfoI RGCGCY 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 43
BseGI GGATG 2 cut(s) 13, 112
BseRI GAGGAG 1 cut(s) 43
BshFI GGCC 1 cut(s) 44
BsnI GGCC 1 cut(s) 44
BspANI GGCC 1 cut(s) 44
BspLI GGNNCC 1 cut(s) 43
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
BstAPI GCANNNNNTGC 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 155
BstF5I GGATG 2 cut(s) 13, 112
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 153
BstMWI GCNNNNNNNGC 2 cut(s) 41, 82
BsuRI GGCC 1 cut(s) 44
BtsCI GGATG 2 cut(s) 13, 112
Cac8I GCNNGC 1 cut(s) 155
CfoI GCGC 2 cut(s) 35, 153
Cfr13I GGNCC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 58
CviAII CATG 1 cut(s) 77
CviJI RGCY 3 cut(s) 44, 97, 157
CviKI_1 RGCY 3 cut(s) 44, 97, 157
CviQI GTAC 1 cut(s) 58
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
Eco47III AGCGCT 1 cut(s) 34
FaeI CATG 1 cut(s) 80
FaiI YATR 1 cut(s) 78
FatI CATG 1 cut(s) 76
FokI GGATG 2 cut(s) 20, 99
FspBI CTAG 2 cut(s) 23, 166
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 2 cut(s) 34, 152
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 1 cut(s) 44
HhaI GCGC 2 cut(s) 35, 153
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 2 cut(s) 33, 151
HinP1I GCGC 2 cut(s) 33, 151
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 1 cut(s) 70
Hpy8I GTNNAC 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 82
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 2 cut(s) 33, 151
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 3 cut(s) 58, 71, 75
MaeI CTAG 2 cut(s) 23, 166
MboII GAAGA 1 cut(s) 100
MluCI AATT 1 cut(s) 133
MnlI CCTC 2 cut(s) 21, 118
MseI TTAA 1 cut(s) 132
MwoI GCNNNNNNNGC 2 cut(s) 41, 82
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 1 cut(s) 43
NsbI TGCGCA 1 cut(s) 152
PshBI ATTAAT 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 1 cut(s) 42
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SaqAI TTAA 1 cut(s) 132
Sau96I GGNCC 1 cut(s) 42
SetI ASST 2 cut(s) 99, 129
SgeI CNNG 9 cut(s) 35, 57, 74, 85, 89, 94, 98, 158, 160
Sse9I AATT 1 cut(s) 133
SspMI CTAG 2 cut(s) 23, 166
TasI AATT 1 cut(s) 133
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 127
VspI ATTAAT 1 cut(s) 132
XspI CTAG 2 cut(s) 23, 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.