Rorug07G0192700

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008309
Physical Location & Seq
Forward (+)
16398633 .. 16399748
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0192700.1

Sequence Viewer

Length: 594 bp
ATGGAATGCCATCTTATGCTAGCTCTGCCTATTCTCCAGTTGCTTCTTTACCACATAAGCCCCTGCACAGCCTCCCCAGCATCTTCTTGCAAAGCAAACACCGAGTACATAAAGACATCATGCAGCGTCACAATCTATCCGAAATTGTGCTACACCTCCCTCTCAATCTACGCAGAAAAAATCAAGACCAACCCGAAAGTGCTAGCTTATACCGCCCTCAATGTGACCCTTGCAGCCACCAAAGCAACTTCTGTGGTAATAAACAGGTGTTCAAGGGTTCATGGACTGAAGCCTATAGAGACTGCAGCTGCACTCGACTGTGTGGAGGAGGTTAGTGACGCTTTGGATGAGCTCCGGATGTCCATAGATGAATTGGGTCATGCAGTTAGAGGCTCGAGAAATTTCTCTTTTCAAATTAGTGATATTCAAACATGGGTTAGTGCTGCATTAACTGATGAGGACACCTGCATGGATGGCTTTGATGAGCATAAATCGACTGGGAGGATGGAGACTGTTGTCCGGAGGGAAATACTGAAAATCGCGCATCTGACGAGCAATGCCTTGGCACTCATCAACAACTATGCCTCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

197

Amino Acids

21.62

Weight (kDa)

6.06

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 36 - 190 2.2e-36 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 1 cut(s) 473
AccII CGCG 1 cut(s) 542
AccIII TCCGGA 2 cut(s) 354, 519
AciI CCGC 1 cut(s) 213
AcsI RAATTY 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 1 cut(s) 107
AgsI TTSAA 3 cut(s) 273, 413, 428
AluBI AGCT 4 cut(s) 23, 206, 308, 352
AluI AGCT 4 cut(s) 23, 206, 308, 352
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 2 cut(s) 293, 503
Ama87I CYCGRG 1 cut(s) 394
Aor13HI TCCGGA 2 cut(s) 354, 519
ApeKI GCWGC 5 cut(s) 123, 233, 305, 308, 443
ApoI RAATTY 1 cut(s) 400
AspLEI GCGC 1 cut(s) 544
AsuNHI GCTAGC 2 cut(s) 19, 202
AvaI CYCGRG 1 cut(s) 394
BanII GRGCYC 1 cut(s) 354
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 5 cut(s) 135, 245, 295, 317, 430
BccI CCATC 3 cut(s) 18, 467, 499
BcoDI GTCTC 2 cut(s) 293, 503
BfaI CTAG 2 cut(s) 20, 203
BfmI CTRYAG 2 cut(s) 294, 303
BfuAI ACCTGC 1 cut(s) 473
BisI GCNGC 5 cut(s) 124, 234, 306, 309, 444
BlsI GCNGC 5 cut(s) 125, 235, 307, 310, 445
BmeT110I CYCGRG 1 cut(s) 394
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 2 cut(s) 89, 553
BmtI GCTAGC 2 cut(s) 23, 206
BmuI ACTGGG 1 cut(s) 507
BoxI GACNNNNGTC 1 cut(s) 515
BpmI CTGGAG 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 561
BsaWI WCCGGW 2 cut(s) 354, 519
Bse1I ACTGG 2 cut(s) 37, 502
Bse3DI GCAATG 1 cut(s) 562
BseAI TCCGGA 2 cut(s) 354, 519
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 4 cut(s) 352, 363, 478, 510
BseMI GCAATG 1 cut(s) 562
BseNI ACTGG 2 cut(s) 37, 502
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 5 cut(s) 135, 245, 295, 317, 430
BseYI CCCAGC 1 cut(s) 76
BsgI GTGCAG 2 cut(s) 49, 294
Bsh1236I CGCG 1 cut(s) 542
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 2 cut(s) 355, 520
BsmAI GTCTC 2 cut(s) 293, 503
BsmI GAATGC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 354
Bsp13I TCCGGA 2 cut(s) 354, 519
BspACI CCGC 1 cut(s) 213
BspEI TCCGGA 2 cut(s) 354, 519
BspFNI CGCG 1 cut(s) 542
BspMAI CTGCAG 1 cut(s) 307
BspMI ACCTGC 1 cut(s) 473
BspOI GCTAGC 2 cut(s) 23, 206
BsrDI GCAATG 1 cut(s) 562
BsrI ACTGG 2 cut(s) 37, 502
BssECI CCNNGG 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 561
Bst4CI ACNGT 2 cut(s) 320, 514
BstC8I GCNNGC 2 cut(s) 21, 204
BstF5I GGATG 4 cut(s) 352, 363, 478, 510
BstFNI CGCG 1 cut(s) 542
BstHHI GCGC 1 cut(s) 544
BstMAI GTCTC 2 cut(s) 293, 503
BstMWI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 474
BstPAI GACNNNNGTC 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 294, 303
BstUI CGCG 1 cut(s) 542
BstV1I GCAGC 5 cut(s) 135, 245, 295, 317, 430
BtsCI GGATG 4 cut(s) 352, 363, 478, 510
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 21, 204
CfoI GCGC 1 cut(s) 544
CseI GACGC 2 cut(s) 115, 347
Csp6I GTAC 1 cut(s) 106
CspCI CAANNNNNGTGG 2 cut(s) 234, 269
CviAII CATG 5 cut(s) 120, 281, 380, 432, 469
CviQI GTAC 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 394
EcoICRI GAGCTC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 5 cut(s) 123, 284, 383, 435, 472
FatI CATG 5 cut(s) 119, 280, 379, 431, 468
Fnu4HI GCNGC 5 cut(s) 124, 234, 306, 309, 444
FokI GGATG 4 cut(s) 359, 370, 485, 517
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 5 cut(s) 124, 234, 306, 309, 444
FspBI CTAG 2 cut(s) 20, 203
GlaI GCGC 1 cut(s) 543
GluI GCNGC 5 cut(s) 124, 234, 306, 309, 444
GsaI CCCAGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 20
HapII CCGG 2 cut(s) 355, 520
HgaI GACGC 2 cut(s) 115, 347
HhaI GCGC 1 cut(s) 544
Hin1II CATG 5 cut(s) 123, 284, 383, 435, 472
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HpaII CCGG 2 cut(s) 355, 520
Hpy188I TCNGA 2 cut(s) 141, 549
Hpy188III TCNNGA 4 cut(s) 184, 355, 396, 520
HpyCH4III ACNGT 2 cut(s) 320, 514
HpyCH4V TGCA 9 cut(s) 66, 90, 123, 233, 305, 311, 383, 446, 468
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 474
Hsp92II CATG 5 cut(s) 123, 284, 383, 435, 472
HspAI GCGC 1 cut(s) 542
Kpn2I TCCGGA 2 cut(s) 354, 519
LmnI GCTCC 1 cut(s) 357
LpnPI CCDG 8 cut(s) 50, 76, 90, 250, 368, 478, 483, 533
Lsp1109I GCAGC 5 cut(s) 135, 245, 295, 317, 430
LweI GCATC 2 cut(s) 89, 553
MaeI CTAG 2 cut(s) 20, 203
MaeIII GTNAC 3 cut(s) 127, 223, 335
MboII GAAGA 1 cut(s) 75
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 4 cut(s) 143, 371, 400, 414
MroI TCCGGA 2 cut(s) 354, 519
MseI TTAA 1 cut(s) 449
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 2 cut(s) 355, 520
Mva1269I GAATGC 1 cut(s) 11
MvnI CGCG 1 cut(s) 542
MwoI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 474
NheI GCTAGC 2 cut(s) 19, 202
NlaIII CATG 5 cut(s) 123, 284, 383, 435, 472
NmuCI GTSAC 3 cut(s) 127, 223, 335
PaeR7I CTCGAG 1 cut(s) 394
PaqCI CACCTGC 1 cut(s) 473
PctI GAATGC 1 cut(s) 11
PkrI GCNGC 5 cut(s) 125, 235, 307, 310, 445
PshAI GACNNNNGTC 1 cut(s) 515
Psp124BI GAGCTC 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 76
PstI CTGCAG 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 5 cut(s) 124, 234, 306, 309, 444
SduI GDGCHC 1 cut(s) 354
SetI ASST 8 cut(s) 25, 158, 208, 269, 310, 333, 354, 467
SfaNI GCATC 2 cut(s) 89, 553
SfcI CTRYAG 2 cut(s) 294, 303
Sfr274I CTCGAG 1 cut(s) 394
SlaI CTCGAG 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 4 cut(s) 143, 371, 400, 414
SsiI CCGC 1 cut(s) 213
SspMI CTAG 2 cut(s) 20, 203
SstI GAGCTC 1 cut(s) 354
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 2 cut(s) 320, 514
TaqI TCGA 4 cut(s) 315, 395, 494, 587
TasI AATT 4 cut(s) 143, 371, 400, 414
TatI WGTACW 1 cut(s) 105
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TseFI GTSAC 3 cut(s) 127, 223, 335
TseI GCWGC 5 cut(s) 123, 233, 305, 308, 443
Tsp45I GTSAC 3 cut(s) 127, 223, 335
TspDTI ATGAA 2 cut(s) 269, 384
XapI RAATTY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 394
XspI CTAG 2 cut(s) 20, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.