Rw7G028290

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008309
Physical Location & Seq
Forward (+)
38373751 .. 38374581
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G028290.1

Sequence Viewer

Length: 831 bp
ATGGAATCCGAAATTCCTGCTGAGCTTCTCATAGCTCACATTATGCAACTCCATGCAAGCATATCCAAGCTCGACTCCCTTCGCCCTTCGAAGCAAGTCAACTCCCTCTTCACCCAGCTGGTCAAGTTGTGCACTCTTCCATCTTCGTTGGATGTTCATGACTTATCCGAAGAGGTCCGAATTATGCGCGAGAGTCTCATCAGTCTCTGCAGCCGAGCCGAAGGTCTGTTGGAGCTTGAGTTTGCCACACTCCTAGGGAAAACCTCTCAACCCATGAACAACCTTAATCTTTTCCCCTACTATGGTAATTATGTCCTGCTTGCCAATTTGGAGAACAAAATTCTTAATGGCAGTGGGGTGGTGCATCCTAACAAGGTCGCCTTTATCGGGTCAGGACCAATGCCTCTCACTTCCATTGTAATGGCTACCCATCACATGAAGTCCGCTCATTTCGATAACTTTGATATCGATGACAAGGCCAATGAGGTGGCTCGCCGAATTGTTGCCTCAGACGCTGATCTAGAGAAGAGGATGACGTTTGAAACTTGTGATATCATGAATGTTCAGGAGAGGCTTGCCGAGTATGATTGCATATTTTTGGCAGCTCTGGTGGGAATGAACAAGGAAGACAAGGTGAAGATTCTCGGGCATATTAGGAAGTATATGAAGGAAGGAAGCTTTTTGCTTGTGAGGAGTGCGAAAGGGGCTAGGGCATTCTTGTACCCTGTTATTGAGGAGCATGACTTGCTGGGCTTTGAGCTTTTGACAATCTTCCATCCCAATAATGAGGTCATAAACTCTGTTGTTCTTGTGCGCAAACCTATCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

276

Amino Acids

31.01

Weight (kDa)

5.94

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 7 - 274 6.4e-105 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 815
AccBSI CCGCTC 1 cut(s) 446
AccII CGCG 1 cut(s) 189
AciI CCGC 1 cut(s) 444
AcsI RAATTY 2 cut(s) 12, 339
AfaI GTAC 1 cut(s) 722
AfiI CCNNNNNNNGG 2 cut(s) 302, 387
AgsI TTSAA 1 cut(s) 542
AluBI AGCT 8 cut(s) 25, 35, 70, 118, 235, 605, 678, 760
AluI AGCT 8 cut(s) 25, 35, 70, 118, 235, 605, 678, 760
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 2 cut(s) 200, 209
Alw44I GTGCAC 1 cut(s) 130
AlwNI CAGNNNCTG 2 cut(s) 207, 515
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 1 cut(s) 477
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 2 cut(s) 210, 602
ApoI RAATTY 2 cut(s) 12, 339
AspA2I CCTAGG 1 cut(s) 253
AspLEI GCGC 2 cut(s) 189, 816
AspS9I GGNCC 2 cut(s) 175, 395
AsuHPI GGTGA 2 cut(s) 103, 646
AsuII TTCGAA 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 2 cut(s) 175, 395
AvrII CCTAGG 1 cut(s) 253
BaeGI GKGCMC 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 2 cut(s) 222, 614
BccI CCATC 3 cut(s) 148, 438, 783
BcgI CGANNNNNNTGC 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 200, 209
BfaI CTAG 3 cut(s) 254, 521, 708
BfmI CTRYAG 1 cut(s) 208
BisI GCNGC 2 cut(s) 211, 603
BlnI CCTAGG 1 cut(s) 253
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 2 cut(s) 212, 604
Bme18I GGWCC 2 cut(s) 175, 395
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 2 cut(s) 175, 395
BmsI GCATC 1 cut(s) 373
BpiI GAAGAC 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 257
Bsa29I ATCGAT 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 253
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 387
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 4 cut(s) 157, 364, 537, 775
BseLI CCNNNNNNNGG 2 cut(s) 302, 387
BseMII CTCAG 2 cut(s) 12, 522
BseRI GAGGAG 2 cut(s) 706, 749
BseSI GKGCMC 1 cut(s) 134
BseXI GCAGC 2 cut(s) 222, 614
BseYI CCCAGC 2 cut(s) 114, 748
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 479
BshVI ATCGAT 1 cut(s) 468
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 644
BslI CCNNNNNNNGG 2 cut(s) 302, 387
BsmAI GTCTC 2 cut(s) 200, 209
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 644
Bsp119I TTCGAA 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 1 cut(s) 517
Bsp1720I GCTNAGC 1 cut(s) 21
BspACI CCGC 1 cut(s) 444
BspANI GGCC 1 cut(s) 479
BspCNI CTCAG 2 cut(s) 13, 521
BspDI ATCGAT 1 cut(s) 468
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 2 cut(s) 157, 555
BspMAI CTGCAG 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 446
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 1 cut(s) 517
BssT1I CCWWGG 1 cut(s) 253
Bst6I CTCTTC 4 cut(s) 113, 141, 165, 521
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBI TTCGAA 1 cut(s) 89
BstC8I GCNNGC 4 cut(s) 58, 321, 493, 576
BstDEI CTNAG 2 cut(s) 21, 508
BstF5I GGATG 4 cut(s) 157, 364, 537, 775
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 2 cut(s) 189, 816
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 2 cut(s) 200, 209
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 3 cut(s) 512, 704, 745
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 134
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 222, 614
BstV2I GAAGAC 1 cut(s) 633
BstXI CCANNNNNNTGG 2 cut(s) 421, 487
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 1 cut(s) 479
BsuTUI ATCGAT 1 cut(s) 468
BtsCI GGATG 4 cut(s) 157, 364, 537, 775
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 358
Cac8I GCNNGC 4 cut(s) 58, 321, 493, 576
CaiI CAGNNNCTG 2 cut(s) 207, 515
CciI TCATGA 2 cut(s) 157, 555
CfoI GCGC 2 cut(s) 189, 816
Cfr13I GGNCC 2 cut(s) 175, 395
ClaI ATCGAT 1 cut(s) 468
CseI GACGC 1 cut(s) 521
Csp6I GTAC 1 cut(s) 721
CviAII CATG 6 cut(s) 53, 158, 274, 436, 556, 740
CviQI GTAC 1 cut(s) 721
DdeI CTNAG 2 cut(s) 21, 508
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
Eam1104I CTCTTC 4 cut(s) 113, 141, 165, 521
EarI CTCTTC 4 cut(s) 113, 141, 165, 521
Eco130I CCWWGG 1 cut(s) 253
Eco32I GATATC 2 cut(s) 466, 553
Eco47I GGWCC 2 cut(s) 175, 395
Eco88I CYCGRG 1 cut(s) 644
EcoRV GATATC 2 cut(s) 466, 553
EcoT14I CCWWGG 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 6 cut(s) 56, 161, 277, 439, 559, 743
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FatI CATG 6 cut(s) 52, 157, 273, 435, 555, 739
Fnu4HI GCNGC 2 cut(s) 211, 603
FokI GGATG 4 cut(s) 164, 351, 544, 762
Fsp4HI GCNGC 2 cut(s) 211, 603
FspBI CTAG 3 cut(s) 254, 521, 708
FspI TGCGCA 1 cut(s) 815
GlaI GCGC 2 cut(s) 188, 815
GluI GCNGC 2 cut(s) 211, 603
GsaI CCCAGC 2 cut(s) 118, 752
HaeIII GGCC 1 cut(s) 479
HgaI GACGC 1 cut(s) 521
HhaI GCGC 2 cut(s) 189, 816
Hin1II CATG 6 cut(s) 56, 161, 277, 439, 559, 743
Hin6I GCGC 2 cut(s) 187, 814
HinP1I GCGC 2 cut(s) 187, 814
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 4 cut(s) 5, 74, 193, 640
HphI GGTGA 2 cut(s) 103, 646
Hpy166II GTNNAC 2 cut(s) 100, 132
Hpy188I TCNGA 4 cut(s) 10, 169, 179, 511
Hpy188III TCNNGA 5 cut(s) 158, 393, 521, 556, 566
Hpy8I GTNNAC 2 cut(s) 100, 132
HpyAV CCTTC 5 cut(s) 89, 96, 215, 661, 665
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 6 cut(s) 46, 56, 132, 210, 364, 591
HpyF10VI GCNNNNNNNGC 3 cut(s) 512, 704, 745
HpyF3I CTNAG 2 cut(s) 21, 508
HpySE526I ACGT 1 cut(s) 536
Hsp92II CATG 6 cut(s) 56, 161, 277, 439, 559, 743
HspAI GCGC 2 cut(s) 187, 814
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 2 cut(s) 232, 736
LpnPI CCDG 9 cut(s) 30, 104, 128, 329, 378, 551, 593, 734, 738
Lsp1109I GCAGC 2 cut(s) 222, 614
LweI GCATC 1 cut(s) 373
MaeI CTAG 3 cut(s) 254, 521, 708
MaeII ACGT 1 cut(s) 536
MalI GATC 1 cut(s) 519
MbiI CCGCTC 1 cut(s) 446
MboI GATC 1 cut(s) 517
MboII GAAGA 8 cut(s) 100, 128, 135, 182, 538, 638, 649, 763
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 6 cut(s) 12, 180, 307, 325, 339, 498
MlyI GAGTC 2 cut(s) 68, 202
MmeI TCCRAC 2 cut(s) 129, 210
MseI TTAA 3 cut(s) 285, 345, 829
MslI CAYNNNNRTG 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 118
Mva1269I GAATGC 1 cut(s) 713
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 3 cut(s) 512, 704, 745
NdeII GATC 1 cut(s) 517
NlaIII CATG 6 cut(s) 56, 161, 277, 439, 559, 743
NmeAIII GCCGAG 2 cut(s) 239, 604
NsbI TGCGCA 1 cut(s) 815
NspV TTCGAA 1 cut(s) 89
PagI TCATGA 2 cut(s) 157, 555
PctI GAATGC 1 cut(s) 713
PfeI GAWTC 2 cut(s) 5, 640
PkrI GCNGC 2 cut(s) 212, 604
PleI GAGTC 2 cut(s) 68, 201
PpsI GAGTC 2 cut(s) 68, 201
PspFI CCCAGC 2 cut(s) 114, 748
PspPI GGNCC 2 cut(s) 175, 395
PstI CTGCAG 1 cut(s) 212
PstNI CAGNNNCTG 2 cut(s) 207, 515
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 1 cut(s) 722
RsaNI GTAC 1 cut(s) 721
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 3 cut(s) 285, 345, 829
SatI GCNGC 2 cut(s) 211, 603
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 2 cut(s) 175, 395
SchI GAGTC 2 cut(s) 68, 202
SduI GDGCHC 1 cut(s) 134
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 89
SinI GGWCC 2 cut(s) 175, 395
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 6 cut(s) 12, 180, 307, 325, 339, 498
SsiI CCGC 1 cut(s) 444
SspMI CTAG 3 cut(s) 254, 521, 708
StyI CCWWGG 1 cut(s) 253
TaiI ACGT 1 cut(s) 539
TaqI TCGA 4 cut(s) 72, 89, 453, 468
TasI AATT 6 cut(s) 12, 180, 307, 325, 339, 498
TfiI GAWTC 2 cut(s) 5, 640
Tru1I TTAA 3 cut(s) 285, 345, 829
Tru9I TTAA 3 cut(s) 285, 345, 829
TscAI CASTG 1 cut(s) 358
TseI GCWGC 2 cut(s) 210, 602
TspDTI ATGAA 6 cut(s) 146, 290, 452, 572, 632, 680
TspRI CASTG 1 cut(s) 358
VneI GTGCAC 1 cut(s) 130
VpaK11BI GGWCC 2 cut(s) 175, 395
XapI RAATTY 2 cut(s) 12, 339
XbaI TCTAGA 1 cut(s) 520
XmaJI CCTAGG 1 cut(s) 253
XspI CTAG 3 cut(s) 254, 521, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.