Rh4DG109000

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008309
Physical Location & Seq
Forward (+)
16756524 .. 16756907
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG109000.1

Sequence Viewer

Length: 384 bp
ATGAACAACCTTAATCTTTTCCCCTACTATGGTAATTATGTTCTGTTGGCGTATTTGGAGAACAAAATTCTTAAAGACAGTGGCGTGGTGCATCCCAACAAGGTCGCCTTTATTGGGTCAGGACCAGTGTCGCTCGCTTCCATTGTAATGGCTACCCATCACATGAAATCCACTCATTTCGATAACTTTGATATCGATGACACGGCCAATGAGGTGGCTCGTCAGATTGTTGCCTCGGACGCTGAGCTAGAGAAGAGGATGAAATTTGAGACTCTTGATATCATGAAAGTTCGGGAGAGGCTTGCCGAGTATGATTGCATATTTTTGGCAGCTCTGGTGGGAATGAACAAAGAAGACAAGTTGAAGATTCTCGGGCATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

127

Amino Acids

14.36

Weight (kDa)

5.91

Isoelectric Point (pI)

25.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 2 - 127 4.5e-46 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 2 cut(s) 66, 263
AfiI CCNNNNNNNGG 2 cut(s) 29, 114
AgsI TTSAA 1 cut(s) 364
AluBI AGCT 2 cut(s) 247, 332
AluI AGCT 2 cut(s) 247, 332
Alw26I GTCTC 1 cut(s) 263
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 329
ApoI RAATTY 2 cut(s) 66, 263
AspS9I GGNCC 1 cut(s) 122
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 360
BbvI GCAGC 1 cut(s) 341
BccI CCATC 1 cut(s) 165
BceAI ACGGC 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 1 cut(s) 248
BisI GCNGC 1 cut(s) 330
BlpI GCTNAGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 331
Bme18I GGWCC 1 cut(s) 122
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 122
BmsI GCATC 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 360
Bpu1102I GCTNAGC 1 cut(s) 243
Bsa29I ATCGAT 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 234
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 114
Bse1I ACTGG 1 cut(s) 125
BseCI ATCGAT 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 2 cut(s) 91, 264
BseLI CCNNNNNNNGG 2 cut(s) 29, 114
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 341
BshFI GGCC 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 371
BslI CCNNNNNNNGG 2 cut(s) 29, 114
BsmAI GTCTC 1 cut(s) 263
BsnI GGCC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 371
Bsp1720I GCTNAGC 1 cut(s) 243
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 235
BspDI ATCGAT 1 cut(s) 195
BspHI TCATGA 1 cut(s) 282
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 80
Bst6I CTCTTC 1 cut(s) 248
BstC8I GCNNGC 2 cut(s) 135, 303
BstDEI CTNAG 1 cut(s) 243
BstF5I GGATG 2 cut(s) 91, 264
BstMAI GTCTC 1 cut(s) 263
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstPAI GACNNNNGTC 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 341
BstV2I GAAGAC 1 cut(s) 360
BstXI CCANNNNNNTGG 2 cut(s) 148, 214
Bsu15I ATCGAT 1 cut(s) 195
BsuRI GGCC 1 cut(s) 206
BsuTUI ATCGAT 1 cut(s) 195
BtsCI GGATG 2 cut(s) 91, 264
BtsIMutI CAGTG 2 cut(s) 85, 132
Cac8I GCNNGC 2 cut(s) 135, 303
CciI TCATGA 1 cut(s) 282
Cfr13I GGNCC 1 cut(s) 122
ClaI ATCGAT 1 cut(s) 195
CseI GACGC 1 cut(s) 248
CviAII CATG 2 cut(s) 163, 283
CviJI RGCY 6 cut(s) 152, 206, 218, 247, 301, 332
CviKI_1 RGCY 6 cut(s) 152, 206, 218, 247, 301, 332
DdeI CTNAG 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Eco32I GATATC 2 cut(s) 193, 280
Eco47I GGWCC 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 371
EcoRV GATATC 2 cut(s) 193, 280
FaeI CATG 2 cut(s) 166, 286
FaiI YATR 9 cut(s) 30, 39, 164, 284, 312, 320, 378, 380, 382
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FatI CATG 2 cut(s) 162, 282
Fnu4HI GCNGC 1 cut(s) 330
FokI GGATG 2 cut(s) 78, 271
Fsp4HI GCNGC 1 cut(s) 330
FspBI CTAG 1 cut(s) 248
GluI GCNGC 1 cut(s) 330
HaeIII GGCC 1 cut(s) 206
HgaI GACGC 1 cut(s) 248
Hin1II CATG 2 cut(s) 166, 286
HinfI GANTC 2 cut(s) 271, 367
Hpy188I TCNGA 2 cut(s) 225, 238
Hpy188III TCNNGA 4 cut(s) 120, 275, 283, 293
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4V TGCA 2 cut(s) 91, 318
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 1 cut(s) 243
Hsp92II CATG 2 cut(s) 166, 286
LpnPI CCDG 3 cut(s) 105, 138, 320
Lsp1109I GCAGC 1 cut(s) 341
LweI GCATC 1 cut(s) 100
MaeI CTAG 1 cut(s) 248
MboII GAAGA 3 cut(s) 265, 365, 376
MluCI AATT 3 cut(s) 34, 66, 263
MlyI GAGTC 1 cut(s) 265
MnlI CCTC 4 cut(s) 205, 244, 249, 291
MseI TTAA 2 cut(s) 12, 72
MslI CAYNNNNRTG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 239
NlaIII CATG 2 cut(s) 166, 286
NmeAIII GCCGAG 1 cut(s) 331
PagI TCATGA 1 cut(s) 282
PfeI GAWTC 1 cut(s) 367
PkrI GCNGC 1 cut(s) 331
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PshAI GACNNNNGTC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 122
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 2 cut(s) 12, 72
SatI GCNGC 1 cut(s) 330
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 1 cut(s) 265
SetI ASST 5 cut(s) 12, 105, 216, 249, 334
SfaNI GCATC 1 cut(s) 100
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 146
Sse9I AATT 3 cut(s) 34, 66, 263
SspMI CTAG 1 cut(s) 248
TaaI ACNGT 1 cut(s) 80
TaqI TCGA 2 cut(s) 180, 195
TasI AATT 3 cut(s) 34, 66, 263
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 2 cut(s) 12, 72
Tru9I TTAA 2 cut(s) 12, 72
TscAI CASTG 2 cut(s) 85, 132
TseI GCWGC 1 cut(s) 329
TspDTI ATGAA 5 cut(s) 17, 179, 275, 299, 359
TspRI CASTG 2 cut(s) 85, 132
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 2 cut(s) 66, 263
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.