Rh7BG324900

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008309
Physical Location & Seq
Reverse (-)
32076250 .. 32085182
8933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG324900.1

Sequence Viewer

Length: 1113 bp
ATGGCTTCCCTCCAGATCACCAAGATGGAATCCGAAATTCCTGCTGAGCTTCTCATAGCTCACATTATGCAACTCCATGCAAGCATATCCAAACTCGACTCCCTTCGCCCTTCGAAGCAAGTCAACTCCCTCTTCACCCAGCTGGTCAAGTTGTGCACTCTTCCATCTTCGTTGGATATTCATGACTTACCCGAAGAGGTCCAAATTATGCGCGAGAGTCTCATTAGTCTCTGCAGCCGAGCTGAAGGTCTGTTAGAGCTTGAGTTTGCCACACTCCTAGGGAAAACCTCTCAACCCATGAACAACCTTAATCTTTTCCCCTACTATGGTAATTATGTCCTGCTGGCCAATTTGGAGAACAAAATTCTTAATGACAGTGGGGTGGTGCATCCTAACAAGGTCGCCTTTATCGGGTCAGGACCAATGCCTCTCACTTCCATTGTAATGGCTACCCATCACATGAAGTCCGCTCATTTCGATAACTTTGATATCGATGACAAGGCCAATGAGGTGGCTCGCCAGATTGTTGCCTCAGACGCTGATCTAGAGAAGAGGATGAAGTTTGAAACTCGTGATATCATGAAAGTTCGGGAGAGGCTTGCCGAGTATGATTGCATATTTTTGGCAGCTCTGGTGGGAATGAACAAGGAAGACAAGGTGAAGATTCTCGGGCATATTAGGAAGTATATGAAGGAAGGAAGCCTTTTGCTTGTGAGGAGTGCGAAAGGGGCTAGGGCATTCTTGTACCCTGTTATTGAGGAGCATGACTTGCTGGGCTTTGAGCTTTTGACAATCTTCCATCCCAATAATGAGGCACAACAAGGAGAGGCAACTATGGTTGTGAAGCTTGCTGACCCTGGACATGCATTTCAACTCTCTTCTTCTTTCCATTTTGCTGGTTTGGATGAGTTGTCGTCTTTTATCCCATTTATTTCGCTGCCTTCAAATGCATTGTCATTTGCTTTTAAGCGTCGCTTCGATCCTTCAGACAAGTTGAGGTTCTATGGTGGCTACGTTCATCTGCCCTTTGGATGTCATCAAGACCAGGTTACAGGTCCACGGCATGCTTACCGGTCAAAGAGGACATCTCTGATTCCCTTTCATCTCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

370

Amino Acids

41.67

Weight (kDa)

6.76

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 15 - 272 3.3e-98 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 470
AccII CGCG 1 cut(s) 213
AciI CCGC 2 cut(s) 468, 1108
AclWI GGATC 1 cut(s) 974
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 2 cut(s) 36, 363
AcuI CTGAAG 2 cut(s) 264, 969
AfaI GTAC 1 cut(s) 746
AfiI CCNNNNNNNGG 2 cut(s) 326, 411
AgeI ACCGGT 1 cut(s) 1071
AgsI TTSAA 3 cut(s) 566, 872, 945
AjnI CCWGG 2 cut(s) 856, 1044
AluBI AGCT 8 cut(s) 49, 59, 142, 242, 259, 629, 784, 847
AluI AGCT 8 cut(s) 49, 59, 142, 242, 259, 629, 784, 847
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 2 cut(s) 224, 233
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 1 cut(s) 974
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 2 cut(s) 345, 501
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 3 cut(s) 234, 626, 937
ApoI RAATTY 2 cut(s) 36, 363
ArsI GACNNNNNNTTYG 2 cut(s) 938, 970
AsiGI ACCGGT 1 cut(s) 1071
AspA2I CCTAGG 1 cut(s) 277
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 3 cut(s) 199, 419, 1055
AsuHPI GGTGA 3 cut(s) 10, 127, 670
AsuII TTCGAA 1 cut(s) 113
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 3 cut(s) 199, 419, 1055
AvrII CCTAGG 1 cut(s) 277
BaeGI GKGCMC 1 cut(s) 158
BalI TGGCCA 1 cut(s) 347
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 158
BbvI GCAGC 3 cut(s) 246, 638, 924
BccI CCATC 4 cut(s) 19, 172, 462, 807
BceAI ACGGC 1 cut(s) 1076
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BciT130I CCWGG 2 cut(s) 858, 1046
BcoDI GTCTC 2 cut(s) 224, 233
BfaI CTAG 4 cut(s) 278, 545, 732, 1111
BfmI CTRYAG 1 cut(s) 232
BisI GCNGC 3 cut(s) 235, 627, 938
BlnI CCTAGG 1 cut(s) 277
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 3 cut(s) 236, 628, 939
Bme1390I CCNGG 2 cut(s) 858, 1046
Bme18I GGWCC 3 cut(s) 199, 419, 1055
BmeT110I CYCGRG 1 cut(s) 668
BmgT120I GGNCC 3 cut(s) 199, 419, 1055
BmrFI CCNGG 2 cut(s) 858, 1046
BmsI GCATC 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 657
BplI GAGNNNNNCTC 2 cut(s) 1072, 1104
Bpu1102I GCTNAGC 1 cut(s) 45
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 281
Bsa29I ATCGAT 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 277, 856, 1058
BsaWI WCCGGW 1 cut(s) 1071
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 411
Bse118I RCCGGY 1 cut(s) 1071
BseBI CCWGG 2 cut(s) 858, 1046
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 277, 856, 1058
BseGI GGATG 5 cut(s) 388, 561, 799, 910, 1037
BseLI CCNNNNNNNGG 2 cut(s) 326, 411
BseMII CTCAG 2 cut(s) 36, 546
BseRI GAGGAG 2 cut(s) 730, 773
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 3 cut(s) 246, 638, 924
BseYI CCCAGC 2 cut(s) 138, 772
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 2 cut(s) 347, 503
BshTI ACCGGT 1 cut(s) 1071
BshVI ATCGAT 1 cut(s) 492
BsiHKAI GWGCWC 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 1 cut(s) 1072
BslI CCNNNNNNNGG 2 cut(s) 326, 411
BsmAI GTCTC 2 cut(s) 224, 233
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 2 cut(s) 347, 503
BsoBI CYCGRG 1 cut(s) 668
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 3 cut(s) 15, 541, 979
Bsp1720I GCTNAGC 1 cut(s) 45
BspACI CCGC 2 cut(s) 468, 1108
BspANI GGCC 2 cut(s) 347, 503
BspCNI CTCAG 2 cut(s) 37, 545
BspDI ATCGAT 1 cut(s) 492
BspFNI CGCG 1 cut(s) 213
BspHI TCATGA 2 cut(s) 181, 579
BspMAI CTGCAG 1 cut(s) 236
BspPI GGATC 1 cut(s) 974
BspT104I TTCGAA 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 1071
BssAI RCCGGY 1 cut(s) 1071
BssECI CCNNGG 3 cut(s) 277, 856, 1058
BssMI GATC 3 cut(s) 15, 541, 979
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 277
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 858, 1046
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 5 cut(s) 137, 165, 189, 545, 883
BstAPI GCANNNNNTGC 1 cut(s) 769
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 6 cut(s) 82, 345, 517, 600, 849, 1065
BstDEI CTNAG 2 cut(s) 45, 532
BstDSI CCRYGG 1 cut(s) 1058
BstF5I GGATG 5 cut(s) 388, 561, 799, 910, 1037
BstFNI CGCG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 3 cut(s) 18, 544, 982
BstMAI GTCTC 2 cut(s) 224, 233
BstMBI GATC 3 cut(s) 15, 541, 979
BstMWI GCNNNNNNNGC 3 cut(s) 536, 728, 769
BstNI CCWGG 2 cut(s) 858, 1046
BstNSI RCATGY 2 cut(s) 866, 1067
BstSCI CCNGG 2 cut(s) 856, 1044
BstSFI CTRYAG 1 cut(s) 232
BstSLI GKGCMC 1 cut(s) 158
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 3 cut(s) 246, 638, 924
BstV2I GAAGAC 1 cut(s) 657
BstXI CCANNNNNNTGG 3 cut(s) 445, 511, 896
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 2 cut(s) 347, 503
BsuTUI ATCGAT 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 1058
BtsCI GGATG 5 cut(s) 388, 561, 799, 910, 1037
BtsIMutI CAGTG 1 cut(s) 382
Cac8I GCNNGC 6 cut(s) 82, 345, 517, 600, 849, 1065
CaiI CAGNNNCTG 1 cut(s) 539
CciI TCATGA 2 cut(s) 181, 579
CfoI GCGC 1 cut(s) 213
Cfr10I RCCGGY 1 cut(s) 1071
Cfr13I GGNCC 3 cut(s) 199, 419, 1055
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 2 cut(s) 545, 959
CsiI ACCWGGT 1 cut(s) 1044
Csp6I GTAC 1 cut(s) 745
CspAI ACCGGT 1 cut(s) 1071
CviAII CATG 8 cut(s) 77, 182, 298, 460, 580, 764, 863, 1064
CviQI GTAC 1 cut(s) 745
DdeI CTNAG 2 cut(s) 45, 532
DpnI GATC 3 cut(s) 17, 543, 981
DpnII GATC 3 cut(s) 15, 541, 979
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 5 cut(s) 137, 165, 189, 545, 883
EarI CTCTTC 5 cut(s) 137, 165, 189, 545, 883
Eco130I CCWWGG 1 cut(s) 277
Eco32I GATATC 2 cut(s) 490, 577
Eco47I GGWCC 3 cut(s) 199, 419, 1055
Eco57I CTGAAG 2 cut(s) 264, 969
Eco88I CYCGRG 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 856, 1044
EcoRV GATATC 2 cut(s) 490, 577
EcoT14I CCWWGG 1 cut(s) 277
EcoT22I ATGCAT 2 cut(s) 868, 952
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 8 cut(s) 80, 185, 301, 463, 583, 767, 866, 1067
FalI AAGNNNNNCTT 6 cut(s) 389, 421, 687, 719, 959, 991
FatI CATG 8 cut(s) 76, 181, 297, 459, 579, 763, 862, 1063
Fnu4HI GCNGC 3 cut(s) 235, 627, 938
FokI GGATG 5 cut(s) 375, 568, 786, 917, 1044
Fsp4HI GCNGC 3 cut(s) 235, 627, 938
FspBI CTAG 4 cut(s) 278, 545, 732, 1111
GlaI GCGC 1 cut(s) 212
GluI GCNGC 3 cut(s) 235, 627, 938
GsaI CCCAGC 2 cut(s) 142, 776
HaeIII GGCC 2 cut(s) 347, 503
HapII CCGG 1 cut(s) 1072
HgaI GACGC 2 cut(s) 545, 959
HhaI GCGC 1 cut(s) 213
Hin1II CATG 8 cut(s) 80, 185, 301, 463, 583, 767, 866, 1067
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 5 cut(s) 29, 98, 217, 664, 1093
HpaII CCGG 1 cut(s) 1072
HphI GGTGA 3 cut(s) 10, 127, 670
Hpy166II GTNNAC 3 cut(s) 124, 156, 1058
Hpy188I TCNGA 4 cut(s) 34, 535, 988, 1092
Hpy188III TCNNGA 8 cut(s) 13, 182, 417, 545, 572, 580, 590, 1040
Hpy8I GTNNAC 3 cut(s) 124, 156, 1058
Hpy99I CGWCG 1 cut(s) 975
HpyAV CCTTC 7 cut(s) 113, 120, 239, 685, 689, 951, 993
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 1 cut(s) 1014
HpyCH4V TGCA 8 cut(s) 70, 80, 156, 234, 388, 615, 866, 950
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 728, 769
HpyF3I CTNAG 2 cut(s) 45, 532
HpySE526I ACGT 1 cut(s) 1014
Hsp92II CATG 8 cut(s) 80, 185, 301, 463, 583, 767, 866, 1067
HspAI GCGC 1 cut(s) 211
Kzo9I GATC 3 cut(s) 15, 541, 979
LmnI GCTCC 1 cut(s) 760
Lsp1109I GCAGC 3 cut(s) 246, 638, 924
LweI GCATC 1 cut(s) 397
MabI ACCWGGT 1 cut(s) 1044
MaeI CTAG 4 cut(s) 278, 545, 732, 1111
MaeII ACGT 1 cut(s) 1014
MaeIII GTNAC 1 cut(s) 1048
MalI GATC 3 cut(s) 17, 543, 981
MbiI CCGCTC 1 cut(s) 470
MboI GATC 3 cut(s) 15, 541, 979
MhlI GDGCHC 1 cut(s) 158
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 5 cut(s) 36, 204, 331, 349, 363
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 2 cut(s) 92, 226
MmeI TCCRAC 1 cut(s) 153
Mox20I TGGCCA 1 cut(s) 347
Mph1103I ATGCAT 2 cut(s) 868, 952
MscI TGGCCA 1 cut(s) 347
MseI TTAA 3 cut(s) 309, 369, 966
MslI CAYNNNNRTG 2 cut(s) 23, 443
Msp20I TGGCCA 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 142
MspI CCGG 1 cut(s) 1072
MspR9I CCNGG 2 cut(s) 858, 1046
Mva1269I GAATGC 1 cut(s) 737
MvaI CCWGG 2 cut(s) 858, 1046
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 3 cut(s) 536, 728, 769
NdeII GATC 3 cut(s) 15, 541, 979
NlaIII CATG 8 cut(s) 80, 185, 301, 463, 583, 767, 866, 1067
NmeAIII GCCGAG 2 cut(s) 263, 628
NsiI ATGCAT 2 cut(s) 868, 952
NspI RCATGY 2 cut(s) 866, 1067
NspV TTCGAA 1 cut(s) 113
PaeI GCATGC 1 cut(s) 1067
PagI TCATGA 2 cut(s) 181, 579
PctI GAATGC 1 cut(s) 737
PfeI GAWTC 3 cut(s) 29, 664, 1093
PinAI ACCGGT 1 cut(s) 1071
PkrI GCNGC 3 cut(s) 236, 628, 939
PleI GAGTC 2 cut(s) 92, 225
PpsI GAGTC 2 cut(s) 92, 225
Psp6I CCWGG 2 cut(s) 856, 1044
PspFI CCCAGC 2 cut(s) 138, 772
PspGI CCWGG 2 cut(s) 856, 1044
PspPI GGNCC 3 cut(s) 199, 419, 1055
PstI CTGCAG 1 cut(s) 236
PstNI CAGNNNCTG 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 142
RsaI GTAC 1 cut(s) 746
RsaNI GTAC 1 cut(s) 745
RseI CAYNNNNRTG 2 cut(s) 23, 443
SaqAI TTAA 3 cut(s) 309, 369, 966
SatI GCNGC 3 cut(s) 235, 627, 938
Sau3AI GATC 3 cut(s) 15, 541, 979
Sau96I GGNCC 3 cut(s) 199, 419, 1055
SchI GAGTC 2 cut(s) 92, 226
ScrFI CCNGG 2 cut(s) 858, 1046
SduI GDGCHC 1 cut(s) 158
SexAI ACCWGGT 1 cut(s) 1044
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 3 cut(s) 199, 419, 1055
SmiMI CAYNNNNRTG 2 cut(s) 23, 443
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SphI GCATGC 1 cut(s) 1067
Sse9I AATT 5 cut(s) 36, 204, 331, 349, 363
SsiI CCGC 2 cut(s) 468, 1108
SspMI CTAG 4 cut(s) 278, 545, 732, 1111
StyD4I CCNGG 2 cut(s) 856, 1044
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 1 cut(s) 1017
TaqI TCGA 5 cut(s) 96, 113, 477, 492, 978
TasI AATT 5 cut(s) 36, 204, 331, 349, 363
TfiI GAWTC 3 cut(s) 29, 664, 1093
Tru1I TTAA 3 cut(s) 309, 369, 966
Tru9I TTAA 3 cut(s) 309, 369, 966
TscAI CASTG 1 cut(s) 382
TseI GCWGC 3 cut(s) 234, 626, 937
TspDTI ATGAA 9 cut(s) 170, 314, 476, 572, 596, 656, 704, 1007, 1091
TspRI CASTG 1 cut(s) 382
VneI GTGCAC 1 cut(s) 154
VpaK11BI GGWCC 3 cut(s) 199, 419, 1055
XapI RAATTY 2 cut(s) 36, 363
XbaI TCTAGA 1 cut(s) 544
XceI RCATGY 2 cut(s) 866, 1067
XmaJI CCTAGG 1 cut(s) 277
XspI CTAG 4 cut(s) 278, 545, 732, 1111
Zsp2I ATGCAT 2 cut(s) 868, 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.