RLG00000002237

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008309
Physical Location & Seq
Forward (+)
30270865 .. 30271781
917 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002237

Sequence Viewer

Length: 801 bp
ATGGAATCCGAAATTCCTGCTGAGCTTCTCATAGCTCACATTATGCAACTCCATGCAAGCATATCCAAGCTCGACTCCCTTCGCCCTTCGAAGCAAGTCAACTCACTCTTCACCCAACTGGTCAAGTTGTGCACTCTTCCATCTTCGTTGGATGTTCATGACTTACCCGAAGAGGTCCAAATTATGCGCGAGAGTCTCATTAGTCTCTGCAGCCGAGCTGAAGGTCTGTTAGAGCTTGAGTTTGCCACACTCCTAGGGAAAACCTCTCAACCCATGAACAACCTTAATCTTTTCCCCTACTATGGTAATTATGTCCTGCTGGCCAATTTGGAGAACAAAATTCTTAATGACAGTGGGGTGGTGCTTCCTAACAAGGTCGCCTTTATCGGGTCAGGACCAATGCCTCTCACTTCCATTGTAATGGCTACCCATCACATGAAGTCCACTCATTTCGATAACTTTGATATCGATGACAAGGCCAATGAGGTGGCTCGCCGGATTGTTGCCTCAGACACTGAGCTAGAGAAGAGGATGAAGTTTGAAACTCAAGATATCATGAAAGTTCGGGAGAGGCTTGCCGAGTATGATTGCATATTTTTGGCAGCTCTGGTGGGAATGAACAAGGAAGACAAGGTGAAGATTCTCGGGCATATTAGGAAGTATATGAAGGAAGGAAGCTTTTTGCTTGTGAGGAGTGCGAAAGGGGCTAGGGCATTCTTGTACCCTGTTATTGAGGAGCATGACTTGCTAGGCTTTGAGCTTTTGACAATCTTCCATCCCAATAATGAGACAATAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

267

Amino Acids

30.06

Weight (kDa)

5.74

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 7 - 266 1.1e-99 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 189
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 2 cut(s) 12, 339
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 1 cut(s) 722
AfiI CCNNNNNNNGG 2 cut(s) 302, 387
AgsI TTSAA 1 cut(s) 542
AluBI AGCT 9 cut(s) 25, 35, 70, 218, 235, 520, 605, 678, 760
AluI AGCT 9 cut(s) 25, 35, 70, 218, 235, 520, 605, 678, 760
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 3 cut(s) 200, 209, 782
Alw44I GTGCAC 1 cut(s) 130
AlwNI CAGNNNCTG 1 cut(s) 515
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 2 cut(s) 321, 477
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 2 cut(s) 210, 602
ApoI RAATTY 2 cut(s) 12, 339
AspA2I CCTAGG 1 cut(s) 253
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 2 cut(s) 175, 395
AsuHPI GGTGA 2 cut(s) 103, 646
AsuII TTCGAA 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 2 cut(s) 175, 395
AvrII CCTAGG 1 cut(s) 253
BaeGI GKGCMC 1 cut(s) 134
BalI TGGCCA 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 2 cut(s) 222, 614
BccI CCATC 3 cut(s) 148, 438, 783
BcgI CGANNNNNNTGC 1 cut(s) 33
BcoDI GTCTC 3 cut(s) 200, 209, 782
BfaI CTAG 4 cut(s) 254, 521, 708, 749
BfmI CTRYAG 1 cut(s) 208
BisI GCNGC 2 cut(s) 211, 603
BlnI CCTAGG 1 cut(s) 253
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 2 cut(s) 212, 604
Bme18I GGWCC 2 cut(s) 175, 395
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 2 cut(s) 175, 395
BpiI GAAGAC 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 89
BpuEI CTTGAG 2 cut(s) 257, 531
Bsa29I ATCGAT 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 253
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 387
Bse1I ACTGG 1 cut(s) 123
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 3 cut(s) 157, 537, 775
BseLI CCNNNNNNNGG 2 cut(s) 302, 387
BseMII CTCAG 3 cut(s) 12, 507, 522
BseNI ACTGG 1 cut(s) 123
BseRI GAGGAG 2 cut(s) 706, 749
BseSI GKGCMC 1 cut(s) 134
BseXI GCAGC 2 cut(s) 222, 614
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 323, 479
BshVI ATCGAT 1 cut(s) 468
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 644
BsiSI CCGG 1 cut(s) 496
BslI CCNNNNNNNGG 2 cut(s) 302, 387
BsmAI GTCTC 3 cut(s) 200, 209, 782
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 2 cut(s) 323, 479
BsoBI CYCGRG 1 cut(s) 644
Bsp119I TTCGAA 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 134
Bsp1720I GCTNAGC 1 cut(s) 21
BspANI GGCC 2 cut(s) 323, 479
BspCNI CTCAG 3 cut(s) 13, 508, 521
BspDI ATCGAT 1 cut(s) 468
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 2 cut(s) 157, 555
BspMAI CTGCAG 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 89
BsrI ACTGG 1 cut(s) 123
BssECI CCNNGG 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 353
Bst6I CTCTTC 4 cut(s) 113, 141, 165, 521
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBI TTCGAA 1 cut(s) 89
BstC8I GCNNGC 4 cut(s) 58, 321, 493, 576
BstDEI CTNAG 3 cut(s) 21, 508, 516
BstF5I GGATG 3 cut(s) 157, 537, 775
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 189
BstMAI GTCTC 3 cut(s) 200, 209, 782
BstMWI GCNNNNNNNGC 2 cut(s) 704, 745
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 134
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 222, 614
BstV2I GAAGAC 1 cut(s) 633
BstXI CCANNNNNNTGG 2 cut(s) 421, 487
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 2 cut(s) 323, 479
BsuTUI ATCGAT 1 cut(s) 468
BtsCI GGATG 3 cut(s) 157, 537, 775
BtsIMutI CAGTG 2 cut(s) 358, 513
Cac8I GCNNGC 4 cut(s) 58, 321, 493, 576
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 2 cut(s) 157, 555
CfoI GCGC 1 cut(s) 189
Cfr13I GGNCC 2 cut(s) 175, 395
ClaI ATCGAT 1 cut(s) 468
Csp6I GTAC 1 cut(s) 721
CviAII CATG 6 cut(s) 53, 158, 274, 436, 556, 740
CviQI GTAC 1 cut(s) 721
DdeI CTNAG 3 cut(s) 21, 508, 516
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 4 cut(s) 113, 141, 165, 521
EarI CTCTTC 4 cut(s) 113, 141, 165, 521
Eco130I CCWWGG 1 cut(s) 253
Eco32I GATATC 2 cut(s) 466, 553
Eco47I GGWCC 2 cut(s) 175, 395
Eco57I CTGAAG 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 644
EcoRV GATATC 2 cut(s) 466, 553
EcoT14I CCWWGG 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 6 cut(s) 56, 161, 277, 439, 559, 743
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FatI CATG 6 cut(s) 52, 157, 273, 435, 555, 739
Fnu4HI GCNGC 2 cut(s) 211, 603
FokI GGATG 3 cut(s) 164, 544, 762
Fsp4HI GCNGC 2 cut(s) 211, 603
FspBI CTAG 4 cut(s) 254, 521, 708, 749
GlaI GCGC 1 cut(s) 188
GluI GCNGC 2 cut(s) 211, 603
HaeIII GGCC 2 cut(s) 323, 479
HapII CCGG 1 cut(s) 496
HhaI GCGC 1 cut(s) 189
Hin1II CATG 6 cut(s) 56, 161, 277, 439, 559, 743
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 4 cut(s) 5, 74, 193, 640
HpaII CCGG 1 cut(s) 496
HphI GGTGA 2 cut(s) 103, 646
Hpy166II GTNNAC 3 cut(s) 100, 132, 444
Hpy188I TCNGA 2 cut(s) 10, 511
Hpy188III TCNNGA 5 cut(s) 158, 393, 548, 556, 566
Hpy8I GTNNAC 3 cut(s) 100, 132, 444
HpyAV CCTTC 5 cut(s) 89, 96, 215, 661, 665
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4V TGCA 5 cut(s) 46, 56, 132, 210, 591
HpyF10VI GCNNNNNNNGC 2 cut(s) 704, 745
HpyF3I CTNAG 3 cut(s) 21, 508, 516
Hsp92II CATG 6 cut(s) 56, 161, 277, 439, 559, 743
HspAI GCGC 1 cut(s) 187
LmnI GCTCC 1 cut(s) 736
LpnPI CCDG 8 cut(s) 30, 104, 305, 329, 378, 509, 593, 738
Lsp1109I GCAGC 2 cut(s) 222, 614
MaeI CTAG 4 cut(s) 254, 521, 708, 749
MboII GAAGA 8 cut(s) 100, 128, 135, 182, 538, 638, 649, 763
MhlI GDGCHC 1 cut(s) 134
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 6 cut(s) 12, 180, 307, 325, 339, 796
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 2 cut(s) 68, 202
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 9 cut(s) 166, 274, 414, 478, 517, 522, 564, 684, 727
Mox20I TGGCCA 1 cut(s) 323
MscI TGGCCA 1 cut(s) 323
MseI TTAA 2 cut(s) 285, 345
MslI CAYNNNNRTG 1 cut(s) 419
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 1 cut(s) 496
Mva1269I GAATGC 1 cut(s) 713
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 704, 745
NlaIII CATG 6 cut(s) 56, 161, 277, 439, 559, 743
NmeAIII GCCGAG 2 cut(s) 239, 604
NspV TTCGAA 1 cut(s) 89
PagI TCATGA 2 cut(s) 157, 555
PctI GAATGC 1 cut(s) 713
PfeI GAWTC 2 cut(s) 5, 640
PkrI GCNGC 2 cut(s) 212, 604
PleI GAGTC 2 cut(s) 68, 201
PpsI GAGTC 2 cut(s) 68, 201
PspPI GGNCC 2 cut(s) 175, 395
PstI CTGCAG 1 cut(s) 212
PstNI CAGNNNCTG 1 cut(s) 515
RsaI GTAC 1 cut(s) 722
RsaNI GTAC 1 cut(s) 721
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 2 cut(s) 285, 345
SatI GCNGC 2 cut(s) 211, 603
Sau96I GGNCC 2 cut(s) 175, 395
SchI GAGTC 2 cut(s) 68, 202
SduI GDGCHC 1 cut(s) 134
SfcI CTRYAG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 89
SinI GGWCC 2 cut(s) 175, 395
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 2 cut(s) 236, 546
SmoI CTYRAG 2 cut(s) 236, 546
Sse9I AATT 6 cut(s) 12, 180, 307, 325, 339, 796
SspMI CTAG 4 cut(s) 254, 521, 708, 749
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 353
TaqI TCGA 4 cut(s) 72, 89, 453, 468
TasI AATT 6 cut(s) 12, 180, 307, 325, 339, 796
TfiI GAWTC 2 cut(s) 5, 640
Tru1I TTAA 2 cut(s) 285, 345
Tru9I TTAA 2 cut(s) 285, 345
TscAI CASTG 2 cut(s) 358, 520
TseI GCWGC 2 cut(s) 210, 602
TspDTI ATGAA 7 cut(s) 146, 290, 452, 548, 572, 632, 680
TspRI CASTG 2 cut(s) 358, 520
VneI GTGCAC 1 cut(s) 130
VpaK11BI GGWCC 2 cut(s) 175, 395
XapI RAATTY 2 cut(s) 12, 339
XmaJI CCTAGG 1 cut(s) 253
XspI CTAG 4 cut(s) 254, 521, 708, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.