FvH4_4g09260

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008309
Physical Location & Seq
Reverse (-)
10804492 .. 10806274
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g09260.t1

Sequence Viewer

Length: 855 bp
ATGGCTTCTCTCCAAATCACCAACATGGAATCCCAAATTCCTGCTGAGCTTCTCATTTCTCACATCATGCAACTCCATGCAAGCATATCCAAGCTCGACTCCCTTCGCCCTTCGAAGCAAGTCAACTCCCTCTTCACTCAGCTGGTCAAGTTGTGCACTCTTCCATCTTCGGTGGATGTTCACGACTTACCCGAAGAGGTCCAAATTATGCGCGAGAGTCTCATCAATCTCTGCGGCCGAGCTGAAGGTCTGTTGGAGCTTGAGTTTGCCACACTCCTAGGCAAAACCTCTCAACCCATGAACAACCTTAATCTTTTCCCCTACTATGGTAATTATGTCCTGCTGGCCAATTTGGAGAACAAAATTCTCAACGACAGTGGGGTGGTGCATCCCAACAAGGTCGCGTTTATCGGGTCAGGACCAATGCCTCTCACTTCCATTGTAATGGCTACCCATCACATGAAGTCGACTCATTTCGATAACTTCGATATCGATGACAAGGCCAATGAGGTGGCTCTCCGGATTGTTGCGTCCGACGCTGAGCTGGAGAAGAGGATGAAGTTTGAGACTCGTGATATCATGAAAGTTCGGGAGAGGCTTGCCGAGTACGATTGCATATTTTTGGCAGCTCTGGTGGGAATGAACAAGGAAGACAAGGTGAAGATTCTGGGGCATATCAGGAAGTATATGAAGGAAGGAAGCTTTTTGCTCGTGAGGAGTGCGAAAGGGGCTAGGGCATTCTTGTACCCTGTTATTGAGGAGCATGATTTGCCGGGCTTCGAGCTTCTGACGATCTTCCATCCCAAAAATGAGGTCATAAACTCTGTTGTTCTTGTGCGCAAGCCTAAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

285

Amino Acids

32.04

Weight (kDa)

6.56

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 15 - 282 1.2e-104 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 839
AccI GTMKAC 1 cut(s) 467
AccII CGCG 2 cut(s) 213, 404
AccIII TCCGGA 1 cut(s) 519
AciI CCGC 1 cut(s) 234
AcoI YGGCCR 2 cut(s) 235, 345
AcsI RAATTY 3 cut(s) 36, 363, 848
AcuI CTGAAG 1 cut(s) 264
AfaI GTAC 2 cut(s) 608, 746
AfiI CCNNNNNNNGG 1 cut(s) 326
AluBI AGCT 9 cut(s) 49, 94, 142, 242, 259, 544, 629, 702, 784
AluI AGCT 9 cut(s) 49, 94, 142, 242, 259, 544, 629, 702, 784
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 2 cut(s) 224, 560
Alw44I GTGCAC 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 519
AoxI GGCC 3 cut(s) 235, 345, 501
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 1 cut(s) 626
ApoI RAATTY 3 cut(s) 36, 363, 848
ArsI GACNNNNNNTTYG 2 cut(s) 798, 830
AspA2I CCTAGG 1 cut(s) 277
AspLEI GCGC 2 cut(s) 213, 840
AspS9I GGNCC 2 cut(s) 199, 419
AsuC2I CCSGG 1 cut(s) 774
AsuHPI GGTGA 2 cut(s) 10, 670
AsuII TTCGAA 1 cut(s) 113
AvaII GGWCC 2 cut(s) 199, 419
AvrII CCTAGG 1 cut(s) 277
BaeGI GKGCMC 1 cut(s) 158
BalI TGGCCA 1 cut(s) 347
BauI CACGAG 2 cut(s) 570, 710
BbsI GAAGAC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 638
BccI CCATC 3 cut(s) 172, 462, 807
BcnI CCSGG 1 cut(s) 774
BcoDI GTCTC 2 cut(s) 224, 560
BfaI CTAG 2 cut(s) 278, 732
BisI GCNGC 2 cut(s) 235, 627
BlnI CCTAGG 1 cut(s) 277
BlpI GCTNAGC 2 cut(s) 45, 540
BlsI GCNGC 2 cut(s) 236, 628
Bme1390I CCNGG 1 cut(s) 774
Bme18I GGWCC 2 cut(s) 199, 419
BmgT120I GGNCC 2 cut(s) 199, 419
BmrFI CCNGG 1 cut(s) 774
BmsI GCATC 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 657
BpmI CTGGAG 1 cut(s) 566
Bpu1102I GCTNAGC 2 cut(s) 45, 540
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 774
Bsa29I ATCGAT 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 277
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 326
BseAI TCCGGA 1 cut(s) 519
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 4 cut(s) 181, 388, 561, 799
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMII CTCAG 3 cut(s) 36, 152, 531
BseRI GAGGAG 2 cut(s) 730, 773
BseSI GKGCMC 1 cut(s) 158
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 1 cut(s) 638
Bsh1236I CGCG 2 cut(s) 213, 404
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 3 cut(s) 237, 347, 503
BshVI ATCGAT 1 cut(s) 492
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 158
BsiSI CCGG 2 cut(s) 520, 773
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 2 cut(s) 224, 560
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 3 cut(s) 237, 347, 503
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 158
Bsp13I TCCGGA 1 cut(s) 519
Bsp143I GATC 1 cut(s) 792
Bsp1720I GCTNAGC 2 cut(s) 45, 540
BspACI CCGC 1 cut(s) 234
BspANI GGCC 3 cut(s) 237, 347, 503
BspCNI CTCAG 3 cut(s) 37, 151, 532
BspDI ATCGAT 1 cut(s) 492
BspEI TCCGGA 1 cut(s) 519
BspFNI CGCG 2 cut(s) 213, 404
BspHI TCATGA 1 cut(s) 579
BspT104I TTCGAA 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 1 cut(s) 792
BssSI CACGAG 2 cut(s) 570, 710
BssT1I CCWWGG 1 cut(s) 277
Bst2BI CACGAG 2 cut(s) 570, 710
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 4 cut(s) 137, 165, 189, 545
BstAPI GCANNNNNTGC 1 cut(s) 769
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 4 cut(s) 82, 345, 600, 842
BstDEI CTNAG 3 cut(s) 45, 138, 540
BstF5I GGATG 4 cut(s) 181, 388, 561, 799
BstFNI CGCG 2 cut(s) 213, 404
BstHHI GCGC 2 cut(s) 213, 840
BstKTI GATC 1 cut(s) 795
BstMAI GTCTC 2 cut(s) 224, 560
BstMBI GATC 1 cut(s) 792
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 3 cut(s) 536, 728, 769
BstSCI CCNGG 1 cut(s) 772
BstSLI GKGCMC 1 cut(s) 158
BstUI CGCG 2 cut(s) 213, 404
BstV1I GCAGC 1 cut(s) 638
BstV2I GAAGAC 1 cut(s) 657
BstXI CCANNNNNNTGG 2 cut(s) 445, 511
BstZI CGGCCG 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 3 cut(s) 237, 347, 503
BsuTUI ATCGAT 1 cut(s) 492
BtsCI GGATG 4 cut(s) 181, 388, 561, 799
BtsIMutI CAGTG 1 cut(s) 382
Cac8I GCNNGC 4 cut(s) 82, 345, 600, 842
CciI TCATGA 1 cut(s) 579
CfoI GCGC 2 cut(s) 213, 840
Cfr13I GGNCC 2 cut(s) 199, 419
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 2 cut(s) 519, 545
Csp6I GTAC 2 cut(s) 607, 745
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 7 cut(s) 25, 67, 77, 298, 460, 580, 764
CviQI GTAC 2 cut(s) 607, 745
DdeI CTNAG 3 cut(s) 45, 138, 540
DpnI GATC 1 cut(s) 794
DpnII GATC 1 cut(s) 792
EaeI YGGCCR 2 cut(s) 235, 345
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 4 cut(s) 137, 165, 189, 545
EarI CTCTTC 4 cut(s) 137, 165, 189, 545
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 277
Eco32I GATATC 2 cut(s) 490, 577
Eco47I GGWCC 2 cut(s) 199, 419
Eco52I CGGCCG 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 264
EcoRV GATATC 2 cut(s) 490, 577
EcoT14I CCWWGG 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 7 cut(s) 28, 70, 80, 301, 463, 583, 767
FatI CATG 7 cut(s) 24, 66, 76, 297, 459, 579, 763
FblI GTMKAC 1 cut(s) 467
Fnu4HI GCNGC 2 cut(s) 235, 627
FokI GGATG 4 cut(s) 188, 375, 568, 786
Fsp4HI GCNGC 2 cut(s) 235, 627
FspBI CTAG 2 cut(s) 278, 732
FspI TGCGCA 1 cut(s) 839
GlaI GCGC 2 cut(s) 212, 839
GluI GCNGC 2 cut(s) 235, 627
GsuI CTGGAG 1 cut(s) 566
HaeIII GGCC 3 cut(s) 237, 347, 503
HapII CCGG 2 cut(s) 520, 773
HgaI GACGC 2 cut(s) 519, 545
HhaI GCGC 2 cut(s) 213, 840
Hin1II CATG 7 cut(s) 28, 70, 80, 301, 463, 583, 767
Hin6I GCGC 2 cut(s) 211, 838
HinP1I GCGC 2 cut(s) 211, 838
HincII GTYRAC 2 cut(s) 124, 468
HindII GTYRAC 2 cut(s) 124, 468
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 6 cut(s) 29, 98, 217, 469, 568, 664
HpaII CCGG 2 cut(s) 520, 773
HphI GGTGA 2 cut(s) 10, 670
Hpy166II GTNNAC 4 cut(s) 124, 156, 181, 468
Hpy188I TCNGA 2 cut(s) 535, 789
Hpy188III TCNNGA 8 cut(s) 182, 417, 520, 572, 580, 590, 679, 712
Hpy8I GTNNAC 4 cut(s) 124, 156, 181, 468
Hpy99I CGWCG 1 cut(s) 539
HpyAV CCTTC 5 cut(s) 113, 120, 239, 685, 689
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4V TGCA 5 cut(s) 70, 80, 156, 388, 615
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 728, 769
HpyF3I CTNAG 3 cut(s) 45, 138, 540
Hsp92II CATG 7 cut(s) 28, 70, 80, 301, 463, 583, 767
HspAI GCGC 2 cut(s) 211, 838
Kpn2I TCCGGA 1 cut(s) 519
Kzo9I GATC 1 cut(s) 792
LmnI GCTCC 2 cut(s) 256, 760
Lsp1109I GCAGC 1 cut(s) 638
LweI GCATC 1 cut(s) 397
MaeI CTAG 2 cut(s) 278, 732
MalI GATC 1 cut(s) 794
MboI GATC 1 cut(s) 792
MboII GAAGA 8 cut(s) 124, 152, 159, 206, 562, 662, 673, 787
MhlI GDGCHC 1 cut(s) 158
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 6 cut(s) 36, 204, 331, 349, 363, 848
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 4 cut(s) 92, 226, 463, 562
MmeI TCCRAC 2 cut(s) 234, 558
Mox20I TGGCCA 1 cut(s) 347
MroI TCCGGA 1 cut(s) 519
MscI TGGCCA 1 cut(s) 347
MseI TTAA 2 cut(s) 309, 853
MslI CAYNNNNRTG 2 cut(s) 23, 443
Msp20I TGGCCA 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 142
MspI CCGG 2 cut(s) 520, 773
MspR9I CCNGG 1 cut(s) 774
Mva1269I GAATGC 1 cut(s) 737
MvnI CGCG 2 cut(s) 213, 404
MwoI GCNNNNNNNGC 3 cut(s) 536, 728, 769
NciI CCSGG 1 cut(s) 774
NdeII GATC 1 cut(s) 792
NlaIII CATG 7 cut(s) 28, 70, 80, 301, 463, 583, 767
NmeAIII GCCGAG 2 cut(s) 263, 628
NsbI TGCGCA 1 cut(s) 839
NspV TTCGAA 1 cut(s) 113
PagI TCATGA 1 cut(s) 579
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 483
PctI GAATGC 1 cut(s) 737
PfeI GAWTC 2 cut(s) 29, 664
PkrI GCNGC 2 cut(s) 236, 628
PleI GAGTC 4 cut(s) 92, 225, 463, 562
PpsI GAGTC 4 cut(s) 92, 225, 463, 562
PspPI GGNCC 2 cut(s) 199, 419
PvuII CAGCTG 1 cut(s) 142
RsaI GTAC 2 cut(s) 608, 746
RsaNI GTAC 2 cut(s) 607, 745
RseI CAYNNNNRTG 2 cut(s) 23, 443
SalI GTCGAC 1 cut(s) 466
SaqAI TTAA 2 cut(s) 309, 853
SatI GCNGC 2 cut(s) 235, 627
Sau3AI GATC 1 cut(s) 792
Sau96I GGNCC 2 cut(s) 199, 419
SchI GAGTC 4 cut(s) 92, 226, 463, 562
ScrFI CCNGG 1 cut(s) 774
SduI GDGCHC 1 cut(s) 158
SfaNI GCATC 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 2 cut(s) 199, 419
SmiMI CAYNNNNRTG 2 cut(s) 23, 443
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 6 cut(s) 36, 204, 331, 349, 363, 848
SsiI CCGC 1 cut(s) 234
SspMI CTAG 2 cut(s) 278, 732
StyD4I CCNGG 1 cut(s) 772
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 377
TaqI TCGA 7 cut(s) 96, 113, 467, 477, 486, 492, 780
TasI AATT 6 cut(s) 36, 204, 331, 349, 363, 848
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 2 cut(s) 29, 664
Tru1I TTAA 2 cut(s) 309, 853
Tru9I TTAA 2 cut(s) 309, 853
TscAI CASTG 1 cut(s) 382
TseI GCWGC 1 cut(s) 626
TspDTI ATGAA 6 cut(s) 314, 476, 572, 596, 656, 704
TspRI CASTG 1 cut(s) 382
VneI GTGCAC 1 cut(s) 154
VpaK11BI GGWCC 2 cut(s) 199, 419
XapI RAATTY 3 cut(s) 36, 363, 848
XmaJI CCTAGG 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 467
XspI CTAG 2 cut(s) 278, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.