AT1G57610

Calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004061
Physical Location & Seq
Reverse (-)
21337125 .. 21339034
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G57610.4

Sequence Viewer

Length: 882 bp
ATGTGGTCGGTGATGGGTTTAGTGAGACGTACGGCTATGTCCTCCACCGTGAATAAAGCTTCTCCGGTTAGGTCTTTGCTTGGTGGGTTTAGATGTCTCAACGTTGAGTCTAAGGAAGAAGATGAGAAAAAGGATATGACTGTGTTAGAAGCTAAGAAGTTAATGAGATTAGTGAATGTTGAAGATATGAAGAAGAAGCTTATTGGTATGGGTGATAAAGAAATGGTTACTTACACTACACTTATTGAAGCATCTCAAGGTTTAGGTATCGCTAGATCTCTTGATGAAGCTCATGCTTTTGCTCGTGTTCTTGATGATGCTGGTGTTATTTTGATCTTTCGTGATAAAGTCTATCTTCATCCTCATAAGGTGGTGGATTTGATTAGAAAGGCAGTGCCTTTAGGTTTGAATCCAGATGATGAGTTGATTAGAGAAGAGTTTGATAAGATGAGAAGTATGAAGGAAGAGATTGATGTGTTAGCTCATCAACAAGTGAGGAAGATTCTATGGGGTGGTCTTGGTTACTCAGTGGTTCAAATTGGAATCTTTGTTAGGCTAACTTTTTGGGAGTTTTCTTGGGATGTGATGGAACCAATAACGTTTTTCACTACAGCTACTGGGATTATTGTGGGTTATGCTTATTTCTTGATGACTTCTAGAGATCCAACGTATCAAGATTTCATGAAAAGGTTGTTTCTTTCCAGGCAGAGGAAGTTGCTTAAGAGTCATAAGTTTGATGCTGAGAGGTTTAAAGAACTGGAGAACAAGTGGAAGATAACGTCTTGCTCTTCTTCTTCGTGCCACGCTAATGCATCGATTCGGAATCGTGTTGGTGTTGATCTTGATTTGGAGGATTCTTTGCAGAGTCATCACAGAGATTGA

Protein Analysis

293

Amino Acids

33.68

Weight (kDa)

8.62

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 95 - 210 5.9e-34 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 2 cut(s) 102, 599
AclWI GGATC 1 cut(s) 656
AfaI GTAC 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 708
AflII CTTAAG 1 cut(s) 719
AgsI TTSAA 4 cut(s) 182, 248, 409, 536
AjnI CCWGG 1 cut(s) 701
AluBI AGCT 6 cut(s) 59, 152, 199, 290, 482, 614
AluI AGCT 6 cut(s) 59, 152, 199, 290, 482, 614
Alw26I GTCTC 2 cut(s) 19, 101
AlwI GGATC 1 cut(s) 656
AlwNI CAGNNNCTG 1 cut(s) 617
AsuHPI GGTGA 2 cut(s) 22, 224
BauI CACGAG 1 cut(s) 303
BccI CCATC 2 cut(s) 7, 580
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 703
BcoDI GTCTC 2 cut(s) 19, 101
BfaI CTAG 2 cut(s) 273, 657
BfmI CTRYAG 1 cut(s) 609
BfrI CTTAAG 1 cut(s) 719
BglII AGATCT 1 cut(s) 275
Bme1390I CCNGG 1 cut(s) 703
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 1 cut(s) 703
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 4 cut(s) 260, 307, 727, 821
BmuI ACTGGG 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 815
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 708
Bse1I ACTGG 2 cut(s) 622, 762
BseBI CCWGG 1 cut(s) 703
BseCI ATCGAT 1 cut(s) 815
BseGI GGATG 2 cut(s) 358, 586
BseLI CCNNNNNNNGG 1 cut(s) 708
BseMII CTCAG 2 cut(s) 540, 732
BseNI ACTGG 2 cut(s) 622, 762
BshVI ATCGAT 1 cut(s) 815
BsiSI CCGG 1 cut(s) 65
BsiWI CGTACG 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 708
BsmAI GTCTC 2 cut(s) 19, 101
BsmBI CGTCTC 1 cut(s) 19
Bsp143I GATC 4 cut(s) 275, 333, 661, 838
BspCNI CTCAG 2 cut(s) 539, 733
BspDI ATCGAT 1 cut(s) 815
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 1 cut(s) 591
BspPI GGATC 1 cut(s) 656
BspQI GCTCTTC 1 cut(s) 793
BspTI CTTAAG 1 cut(s) 719
BsrI ACTGG 2 cut(s) 622, 762
BssMI GATC 4 cut(s) 275, 333, 661, 838
BssSI CACGAG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 703
Bst4CI ACNGT 2 cut(s) 49, 142
Bst6I CTCTTC 3 cut(s) 429, 459, 793
BstAFI CTTAAG 1 cut(s) 719
BstDEI CTNAG 4 cut(s) 111, 153, 526, 741
BstF5I GGATG 2 cut(s) 358, 586
BstKTI GATC 4 cut(s) 278, 336, 664, 841
BstMAI GTCTC 2 cut(s) 19, 101
BstMBI GATC 4 cut(s) 275, 333, 661, 838
BstNI CCWGG 1 cut(s) 703
BstSCI CCNGG 1 cut(s) 701
BstSFI CTRYAG 1 cut(s) 609
BstX2I RGATCY 2 cut(s) 275, 661
BstYI RGATCY 2 cut(s) 275, 661
Bsu15I ATCGAT 1 cut(s) 815
BsuTUI ATCGAT 1 cut(s) 815
BtsCI GGATG 2 cut(s) 358, 586
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 399, 534
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 1 cut(s) 681
ClaI ATCGAT 1 cut(s) 815
Csp6I GTAC 1 cut(s) 30
CviAII CATG 2 cut(s) 293, 682
CviJI RGCY 8 cut(s) 35, 59, 152, 199, 290, 482, 556, 614
CviKI_1 RGCY 8 cut(s) 35, 59, 152, 199, 290, 482, 556, 614
CviQI GTAC 1 cut(s) 30
DdeI CTNAG 4 cut(s) 111, 153, 526, 741
DpnI GATC 4 cut(s) 277, 335, 663, 840
DpnII GATC 4 cut(s) 275, 333, 661, 838
DraI TTTAAA 1 cut(s) 751
Eam1104I CTCTTC 3 cut(s) 429, 459, 793
EarI CTCTTC 3 cut(s) 429, 459, 793
EcoRII CCWGG 1 cut(s) 701
EcoT22I ATGCAT 1 cut(s) 814
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 2 cut(s) 296, 685
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FatI CATG 2 cut(s) 292, 681
FokI GGATG 2 cut(s) 345, 593
FspBI CTAG 2 cut(s) 273, 657
GsuI CTGGAG 1 cut(s) 779
HapII CCGG 1 cut(s) 65
Hin1II CATG 2 cut(s) 296, 685
HindIII AAGCTT 2 cut(s) 57, 197
HinfI GANTC 9 cut(s) 107, 409, 502, 543, 724, 817, 823, 854, 865
HpaII CCGG 1 cut(s) 65
HphI GGTGA 2 cut(s) 22, 224
Hpy188I TCNGA 1 cut(s) 822
Hpy188III TCNNGA 9 cut(s) 281, 311, 341, 413, 646, 657, 674, 682, 842
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 2 cut(s) 49, 142
HpyCH4IV ACGT 5 cut(s) 28, 102, 599, 668, 779
HpyCH4V TGCA 2 cut(s) 812, 862
HpyF3I CTNAG 4 cut(s) 111, 153, 526, 741
HpySE526I ACGT 5 cut(s) 28, 102, 599, 668, 779
Hsp92II CATG 2 cut(s) 296, 685
Kzo9I GATC 4 cut(s) 275, 333, 661, 838
LguI GCTCTTC 1 cut(s) 793
LpnPI CCDG 7 cut(s) 78, 306, 426, 603, 688, 715, 743
LweI GCATC 4 cut(s) 260, 307, 727, 821
MaeI CTAG 2 cut(s) 273, 657
MaeII ACGT 5 cut(s) 28, 102, 599, 668, 779
MaeIII GTNAC 2 cut(s) 226, 521
MalI GATC 4 cut(s) 277, 335, 663, 840
MboI GATC 4 cut(s) 275, 333, 661, 838
MflI RGATCY 2 cut(s) 275, 661
MluCI AATT 1 cut(s) 537
MlyI GAGTC 3 cut(s) 116, 733, 874
MmeI TCCRAC 1 cut(s) 689
MnlI CCTC 6 cut(s) 52, 372, 489, 702, 738, 844
Mph1103I ATGCAT 1 cut(s) 814
MseI TTAA 3 cut(s) 161, 720, 750
MslI CAYNNNNRTG 1 cut(s) 807
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 703
MvaI CCWGG 1 cut(s) 703
NdeII GATC 4 cut(s) 275, 333, 661, 838
NlaIII CATG 2 cut(s) 296, 685
NlaIV GGNNCC 1 cut(s) 591
NsiI ATGCAT 1 cut(s) 814
PagI TCATGA 1 cut(s) 681
PciSI GCTCTTC 1 cut(s) 793
PfeI GAWTC 6 cut(s) 409, 502, 543, 817, 823, 854
Pfl23II CGTACG 1 cut(s) 29
PleI GAGTC 3 cut(s) 115, 732, 873
PpsI GAGTC 3 cut(s) 115, 732, 873
Psp1406I AACGTT 2 cut(s) 102, 599
Psp6I CCWGG 1 cut(s) 701
PspGI CCWGG 1 cut(s) 701
PspLI CGTACG 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 591
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 2 cut(s) 275, 661
RsaI GTAC 1 cut(s) 31
RsaNI GTAC 1 cut(s) 30
RseI CAYNNNNRTG 1 cut(s) 807
SapI GCTCTTC 1 cut(s) 793
SaqAI TTAA 3 cut(s) 161, 720, 750
Sau3AI GATC 4 cut(s) 275, 333, 661, 838
SchI GAGTC 3 cut(s) 116, 733, 874
ScrFI CCNGG 1 cut(s) 703
SfaNI GCATC 4 cut(s) 260, 307, 727, 821
SfcI CTRYAG 1 cut(s) 609
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 2 cut(s) 255, 719
SmoI CTYRAG 2 cut(s) 255, 719
Sse9I AATT 1 cut(s) 537
SspMI CTAG 2 cut(s) 273, 657
StyD4I CCNGG 1 cut(s) 701
TaaI ACNGT 2 cut(s) 49, 142
TaiI ACGT 5 cut(s) 31, 105, 602, 671, 782
TaqI TCGA 1 cut(s) 815
TasI AATT 1 cut(s) 537
TfiI GAWTC 6 cut(s) 409, 502, 543, 817, 823, 854
Tru1I TTAA 3 cut(s) 161, 720, 750
Tru9I TTAA 3 cut(s) 161, 720, 750
TscAI CASTG 2 cut(s) 399, 534
TspDTI ATGAA 6 cut(s) 203, 300, 347, 473, 670, 698
TspRI CASTG 2 cut(s) 399, 534
Vha464I CTTAAG 1 cut(s) 719
XbaI TCTAGA 1 cut(s) 656
XspI CTAG 2 cut(s) 273, 657
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.