pycom03g03630

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0004061
Physical Location & Seq
Forward (+)
2890197 .. 2892063
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03630.1

Sequence Viewer

Length: 924 bp
ATGTGGAGGTGGTGCAGTGGTGTGTTGGTGAGACATTTGGGGGTGGGGAAGCCCAACGGCGCCAACCCCTGTTTTGGATTCAAGGATTTTGCTAACCGGGAATGTGGGTTGTTGAATTTTCCTGCAACTTCGTCTCTGGCGCTGATTCCGAGGAGAAGAATGTCGTCTTCTAGTGATTTCAGCGGCGTTGGGGGTGGCGGAGTAGGAGGAGGAGGAGCAGATCTGAGCAGAGGAGTAGGAGAGACGATATCGTCTTCGGAGGCCAAGAGGATGATGAGGCTGGTGAATGTGGAGGCTTTGAAGGCAAAGCTCGGGACAGAAGGGAAGGAGGCAATACCGTATTCTGATCTTCTGGAGGCGTGCCAGAGCATTGGGGTTGCCAGATCTCCGGAGGAGGCTGCGGCTTTTGCTCGGGTTCTCGACGAGGCCGGCGTCATCATCCTCTTCCGGGACAAGGTTCTCCTCCATCCTAATAGGGTTGTGGAGCTAGTTCGAAAAGCAGTACCCCTTTCTTTGACTCCGGAAGATGACCCCTTGTGGGAGGAGTTAAAACAGCTGCAGAAGAAGAAGGAAGAAATTGATGTGCTGGCACATAAGCATGTCCGGCGCATTCTCTGGTTTGGCCTGGGGGTTGTCCTGACACAGCTCGGGCTCTTTTTCAGGCTAACATATTGGGACTTCTCATGGGACGTAGTGGAACCACTCGCGTACTTCACAACGACTTCTTGCATTGGCATAGGCTATGCCTACTTCTTGATCACGTCAAGAGATCCGACTTACCAAGATCTGATGAAGAGGCTCTTTATCAGGAGGCAGAGGAAACTGATCAAGAGTCATAATTTTGATGTCAAAAAGTTCAAGGATCGATCCAGAGAAAATGCGGCATACCTCTACACGCTAGTGCCTCGATTAAGAACCGGGTAG

Protein Analysis

308

Amino Acids

34.43

Weight (kDa)

9.55

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 131 - 287 1.9e-48 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 250
AccB1I GGYRCC 1 cut(s) 59
AccII CGCG 1 cut(s) 707
AccIII TCCGGA 2 cut(s) 388, 520
AciI CCGC 4 cut(s) 183, 198, 401, 881
AclWI GGATC 3 cut(s) 764, 861, 870
AcsI RAATTY 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 60, 432
AfaI GTAC 2 cut(s) 504, 710
AfiI CCNNNNNNNGG 4 cut(s) 74, 448, 454, 538
AgsI TTSAA 4 cut(s) 82, 115, 301, 859
AjiI CACGTC 1 cut(s) 762
AjnI CCWGG 1 cut(s) 624
AleI CACNNNNGTG 1 cut(s) 899
AluBI AGCT 4 cut(s) 310, 487, 556, 646
AluI AGCT 4 cut(s) 310, 487, 556, 646
Alw26I GTCTC 3 cut(s) 25, 138, 236
AlwI GGATC 3 cut(s) 764, 861, 870
Ama87I CYCGRG 3 cut(s) 311, 411, 647
Aor13HI TCCGGA 2 cut(s) 388, 520
AoxI GGCC 3 cut(s) 261, 426, 622
ApeKI GCWGC 2 cut(s) 398, 556
ApoI RAATTY 1 cut(s) 115
AspLEI GCGC 3 cut(s) 62, 142, 609
AsuC2I CCSGG 3 cut(s) 98, 449, 919
AsuHPI GGTGA 2 cut(s) 40, 295
AsuII TTCGAA 1 cut(s) 493
AvaI CYCGRG 3 cut(s) 311, 411, 647
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 654
BbsI GAAGAC 2 cut(s) 159, 246
BbvI GCAGC 2 cut(s) 385, 543
BccI CCATC 1 cut(s) 474
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 626
BclI TGATCA 2 cut(s) 756, 825
BcnI CCSGG 3 cut(s) 98, 449, 919
BcoDI GTCTC 3 cut(s) 25, 138, 236
BfaI CTAG 3 cut(s) 171, 488, 899
BfmI CTRYAG 1 cut(s) 557
BfoI RGCGCY 2 cut(s) 63, 143
BglII AGATCT 3 cut(s) 220, 383, 784
BisI GCNGC 5 cut(s) 184, 399, 402, 557, 882
BlsI GCNGC 5 cut(s) 185, 400, 403, 558, 883
Bme1390I CCNGG 4 cut(s) 98, 449, 626, 919
BmeT110I CYCGRG 3 cut(s) 311, 411, 647
BmgBI CACGTC 1 cut(s) 762
BmiI GGNNCC 2 cut(s) 61, 699
BmrFI CCNGG 4 cut(s) 98, 449, 626, 919
BpiI GAAGAC 2 cut(s) 159, 246
BpmI CTGGAG 1 cut(s) 374
Bpu14I TTCGAA 1 cut(s) 493
BpuMI CCSGG 3 cut(s) 98, 449, 919
Bsa29I ATCGAT 1 cut(s) 865
BsaHI GRCGYC 2 cut(s) 60, 432
BsaJI CCNNGG 2 cut(s) 149, 625
BsaWI WCCGGW 2 cut(s) 388, 520
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 448, 454, 538
Bse118I RCCGGY 1 cut(s) 428
BseAI TCCGGA 2 cut(s) 388, 520
BseBI CCWGG 1 cut(s) 626
BseCI ATCGAT 1 cut(s) 865
BseDI CCNNGG 2 cut(s) 149, 625
BseGI GGATG 3 cut(s) 276, 438, 466
BseLI CCNNNNNNNGG 4 cut(s) 74, 448, 454, 538
BseMII CTCAG 1 cut(s) 215
BseRI GAGGAG 8 cut(s) 166, 222, 225, 228, 246, 407, 452, 557
BseXI GCAGC 2 cut(s) 385, 543
BsgI GTGCAG 1 cut(s) 34
Bsh1236I CGCG 1 cut(s) 707
BshFI GGCC 3 cut(s) 263, 428, 624
BshNI GGYRCC 1 cut(s) 59
BshVI ATCGAT 1 cut(s) 865
BsiHKCI CYCGRG 3 cut(s) 311, 411, 647
BsiSI CCGG 7 cut(s) 97, 389, 429, 448, 521, 604, 918
BslFI GGGAC 4 cut(s) 328, 464, 689, 701
BslI CCNNNNNNNGG 4 cut(s) 74, 448, 454, 538
BsmAI GTCTC 3 cut(s) 25, 138, 236
BsmBI CGTCTC 2 cut(s) 138, 236
BsmFI GGGAC 4 cut(s) 328, 464, 689, 701
BsmI GAATGC 1 cut(s) 609
BsnI GGCC 3 cut(s) 263, 428, 624
BsoBI CYCGRG 3 cut(s) 311, 411, 647
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 1 cut(s) 654
Bsp13I TCCGGA 2 cut(s) 388, 520
Bsp143I GATC 9 cut(s) 220, 346, 383, 756, 769, 784, 825, 862, 866
BspACI CCGC 4 cut(s) 183, 198, 401, 881
BspANI GGCC 3 cut(s) 263, 428, 624
BspCNI CTCAG 1 cut(s) 216
BspDI ATCGAT 1 cut(s) 865
BspEI TCCGGA 2 cut(s) 388, 520
BspFNI CGCG 1 cut(s) 707
BspLI GGNNCC 2 cut(s) 61, 699
BspMAI CTGCAG 1 cut(s) 561
BspPI GGATC 3 cut(s) 764, 861, 870
BspT104I TTCGAA 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 59
BsrFI RCCGGY 1 cut(s) 428
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 2 cut(s) 149, 625
BssMI GATC 9 cut(s) 220, 346, 383, 756, 769, 784, 825, 862, 866
BssNI GRCGYC 2 cut(s) 60, 432
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 1 cut(s) 339
Bst6I CTCTTC 2 cut(s) 449, 788
BstACI GRCGYC 2 cut(s) 60, 432
BstBI TTCGAA 1 cut(s) 493
BstC8I GCNNGC 3 cut(s) 361, 430, 588
BstDEI CTNAG 1 cut(s) 224
BstF5I GGATG 3 cut(s) 276, 438, 466
BstFNI CGCG 1 cut(s) 707
BstH2I RGCGCY 2 cut(s) 63, 143
BstHHI GCGC 3 cut(s) 62, 142, 609
BstKTI GATC 9 cut(s) 223, 349, 386, 759, 772, 787, 828, 865, 869
BstMAI GTCTC 3 cut(s) 25, 138, 236
BstMBI GATC 9 cut(s) 220, 346, 383, 756, 769, 784, 825, 862, 866
BstMWI GCNNNNNNNGC 3 cut(s) 302, 407, 604
BstNI CCWGG 1 cut(s) 626
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 4 cut(s) 96, 447, 624, 917
BstSFI CTRYAG 1 cut(s) 557
BstUI CGCG 1 cut(s) 707
BstV1I GCAGC 2 cut(s) 385, 543
BstV2I GAAGAC 2 cut(s) 159, 246
BstX2I RGATCY 4 cut(s) 220, 383, 769, 784
BstXI CCANNNNNNTGG 1 cut(s) 371
BstYI RGATCY 4 cut(s) 220, 383, 769, 784
Bsu15I ATCGAT 1 cut(s) 865
BsuRI GGCC 3 cut(s) 263, 428, 624
BsuTUI ATCGAT 1 cut(s) 865
BtrI CACGTC 1 cut(s) 762
BtsCI GGATG 3 cut(s) 276, 438, 466
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 22
Cac8I GCNNGC 3 cut(s) 361, 430, 588
CfoI GCGC 3 cut(s) 62, 142, 609
Cfr10I RCCGGY 1 cut(s) 428
ClaI ATCGAT 1 cut(s) 865
CseI GACGC 1 cut(s) 421
Csp6I GTAC 2 cut(s) 503, 709
CviAII CATG 2 cut(s) 599, 684
CviQI GTAC 2 cut(s) 503, 709
DdeI CTNAG 1 cut(s) 224
DinI GGCGCC 1 cut(s) 61
DpnI GATC 9 cut(s) 222, 348, 385, 758, 771, 786, 827, 864, 868
DpnII GATC 9 cut(s) 220, 346, 383, 756, 769, 784, 825, 862, 866
DrdI GACNNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 449, 788
EarI CTCTTC 2 cut(s) 449, 788
EciI GGCGGA 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 654
Eco32I GATATC 1 cut(s) 249
Eco88I CYCGRG 3 cut(s) 311, 411, 647
EcoRII CCWGG 1 cut(s) 624
EcoRV GATATC 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 654
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
Esp3I CGTCTC 2 cut(s) 138, 236
FaeI CATG 2 cut(s) 602, 687
FaiI YATR 8 cut(s) 594, 600, 670, 685, 737, 744, 837, 886
FalI AAGNNNNNCTT 2 cut(s) 785, 817
FaqI GGGAC 4 cut(s) 328, 464, 689, 701
FatI CATG 2 cut(s) 598, 683
FbaI TGATCA 2 cut(s) 756, 825
Fnu4HI GCNGC 5 cut(s) 184, 399, 402, 557, 882
FokI GGATG 3 cut(s) 283, 425, 453
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 5 cut(s) 184, 399, 402, 557, 882
FspBI CTAG 3 cut(s) 171, 488, 899
GlaI GCGC 3 cut(s) 61, 141, 608
GluI GCNGC 5 cut(s) 184, 399, 402, 557, 882
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 2 cut(s) 63, 143
HaeIII GGCC 3 cut(s) 263, 428, 624
HapII CCGG 7 cut(s) 97, 389, 429, 448, 521, 604, 918
HgaI GACGC 1 cut(s) 421
HhaI GCGC 3 cut(s) 62, 142, 609
Hin1I GRCGYC 2 cut(s) 60, 432
Hin1II CATG 2 cut(s) 602, 687
Hin6I GCGC 3 cut(s) 60, 140, 607
HinP1I GCGC 3 cut(s) 60, 140, 607
HinfI GANTC 4 cut(s) 78, 145, 517, 832
HpaII CCGG 7 cut(s) 97, 389, 429, 448, 521, 604, 918
HphI GGTGA 2 cut(s) 40, 295
Hpy188I TCNGA 6 cut(s) 150, 225, 259, 346, 774, 789
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 4 cut(s) 295, 314, 319, 562
HpyCH4III ACNGT 1 cut(s) 339
HpyCH4IV ACGT 2 cut(s) 690, 761
HpyCH4V TGCA 4 cut(s) 15, 125, 559, 729
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 407, 604
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 2 cut(s) 690, 761
Hsp92I GRCGYC 2 cut(s) 60, 432
Hsp92II CATG 2 cut(s) 602, 687
HspAI GCGC 3 cut(s) 60, 140, 607
KasI GGCGCC 1 cut(s) 59
Kpn2I TCCGGA 2 cut(s) 388, 520
KroI GCCGGC 1 cut(s) 428
KroNI GCCGGC 1 cut(s) 430
Ksp22I TGATCA 2 cut(s) 756, 825
Kzo9I GATC 9 cut(s) 220, 346, 383, 756, 769, 784, 825, 862, 866
LmnI GCTCC 2 cut(s) 215, 484
Lsp1109I GCAGC 2 cut(s) 385, 543
MaeI CTAG 3 cut(s) 171, 488, 899
MaeII ACGT 2 cut(s) 690, 761
MalI GATC 9 cut(s) 222, 348, 385, 758, 771, 786, 827, 864, 868
MboI GATC 9 cut(s) 220, 346, 383, 756, 769, 784, 825, 862, 866
MflI RGATCY 4 cut(s) 220, 383, 769, 784
MhlI GDGCHC 1 cut(s) 654
MluCI AATT 3 cut(s) 115, 576, 838
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 2 cut(s) 511, 841
MmeI TCCRAC 1 cut(s) 797
MroI TCCGGA 2 cut(s) 388, 520
MroNI GCCGGC 1 cut(s) 428
MseI TTAA 2 cut(s) 548, 911
MslI CAYNNNNRTG 2 cut(s) 597, 899
MspA1I CMGCKG 2 cut(s) 183, 556
MspI CCGG 7 cut(s) 97, 389, 429, 448, 521, 604, 918
MspR9I CCNGG 4 cut(s) 98, 449, 626, 919
Mva1269I GAATGC 1 cut(s) 609
MvaI CCWGG 1 cut(s) 626
MvnI CGCG 1 cut(s) 707
MwoI GCNNNNNNNGC 3 cut(s) 302, 407, 604
NaeI GCCGGC 1 cut(s) 430
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 3 cut(s) 98, 449, 919
NdeII GATC 9 cut(s) 220, 346, 383, 756, 769, 784, 825, 862, 866
NgoMIV GCCGGC 1 cut(s) 428
NlaIII CATG 2 cut(s) 602, 687
NlaIV GGNNCC 2 cut(s) 61, 699
NspI RCATGY 1 cut(s) 602
NspV TTCGAA 1 cut(s) 493
OliI CACNNNNGTG 1 cut(s) 899
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PctI GAATGC 1 cut(s) 609
PdiI GCCGGC 1 cut(s) 430
PfeI GAWTC 2 cut(s) 78, 145
PfoI TCCNGGA 1 cut(s) 447
PkrI GCNGC 5 cut(s) 185, 400, 403, 558, 883
PleI GAGTC 2 cut(s) 511, 840
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 2 cut(s) 511, 840
Psp6I CCWGG 1 cut(s) 624
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 2 cut(s) 61, 699
PstI CTGCAG 1 cut(s) 561
PsuI RGATCY 4 cut(s) 220, 383, 769, 784
PvuII CAGCTG 1 cut(s) 556
RsaI GTAC 2 cut(s) 504, 710
RsaNI GTAC 2 cut(s) 503, 709
RseI CAYNNNNRTG 2 cut(s) 597, 899
SaqAI TTAA 2 cut(s) 548, 911
SatI GCNGC 5 cut(s) 184, 399, 402, 557, 882
Sau3AI GATC 9 cut(s) 220, 346, 383, 756, 769, 784, 825, 862, 866
SchI GAGTC 2 cut(s) 511, 841
ScrFI CCNGG 4 cut(s) 98, 449, 626, 919
SduI GDGCHC 1 cut(s) 654
SetI ASST 9 cut(s) 11, 312, 459, 489, 558, 648, 693, 764, 891
SfcI CTRYAG 1 cut(s) 557
SfoI GGCGCC 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 493
SmiMI CAYNNNNRTG 2 cut(s) 597, 899
Sse9I AATT 3 cut(s) 115, 576, 838
SsiI CCGC 4 cut(s) 183, 198, 401, 881
SspDI GGCGCC 1 cut(s) 59
SspMI CTAG 3 cut(s) 171, 488, 899
StyD4I CCNGG 4 cut(s) 96, 447, 624, 917
TaaI ACNGT 1 cut(s) 339
TaiI ACGT 2 cut(s) 693, 764
TaqI TCGA 4 cut(s) 420, 493, 865, 907
TasI AATT 3 cut(s) 115, 576, 838
TauI GCSGC 3 cut(s) 186, 404, 884
TfiI GAWTC 2 cut(s) 78, 145
Tru1I TTAA 2 cut(s) 548, 911
Tru9I TTAA 2 cut(s) 548, 911
TscAI CASTG 1 cut(s) 22
TseI GCWGC 2 cut(s) 398, 556
TspDTI ATGAA 1 cut(s) 806
TspRI CASTG 1 cut(s) 22
XapI RAATTY 1 cut(s) 115
XceI RCATGY 1 cut(s) 602
XspI CTAG 3 cut(s) 171, 488, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.