MD03G1046100.v1.1

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004061
Physical Location & Seq
Forward (+)
3714478 .. 3717144
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1046100.v1.1.491

Sequence Viewer

Length: 966 bp
ATGTGGAGGTGGTGCAGTGGTGTGTTGGTGAGACATTTGGGGGTGGGGAAGCCCAACGGCGCCAACCCATGTTTTGGATTCAAGGGTTTTGCTAACCGGGAATGTGGGTTGTTGAATATTCCTGCAACTTCGTCTCTGGCGCTGATTCCGAGGAGAAGAATGTCGTCTTCTAATGATTTCAGCAGCGGTGGGGGTGGCGGAGTAGGAGGAGGAGGAGGAGCAGATCTGGGCAGAGGAGTAGGAGAGACGATATCGTCTTCGGAGGCCAAGAGGATGATGAGGCTGGTGAATGTGGAGGCTTTGAAGGCAAAGCTTGGGACAGAAGGGAAAGAGGCAATACCGTATTCTGATCTTCTGGAGGCGTGCCAGAGCATTGGGGTTGCCAGATCTCCGGAGGAGGCTGCGGCTTTTGCTCGGGTTCTCGACGAGGCCGGCGTCATCATCCTCTTCCGGGACAAGGTTCTCCTCCATCCTAATAGGGTTGTGGAGCTAGTTCGAAAAGCAGTACCCCTTTCTTTGACTCCAGAAGATGACCCCTTGTGGGAGGAGTTAAAACAGCTGCAGAAGAAGAAGGAAGAAATTGATGTACTGGCACATAAGCATGTCCGGCGCGTTCTCTGGTTTGGCCTGGGGGTTGTCCTGGCACAGCTCGGGCTCTTTTTCAGGCTAACATATTGGGACTTCTCATGGGACGTAGTGGAACCACTCGCGTACTTCACTACGACTTCTTACATTGGCATAGGCTATGCCTACTTCTTGATCACGTCAAGGGATCCGACTTACCAAGATCTGATGAAGAGGCTCTTTATCAGGAGGCAGAGGAAACTGATCAAGCGTCATAATTTTGATGTCGAAAAGTTCAAGGAGATCCAGAGAAAATGCGGCATACCTCTACACGCCAGTGCCTCGCTTAAGAATCGGGTAGGCCTGGAAATAGAGCTCGACGACTCTTTACATAGAGATTAA

Protein Analysis

322

Amino Acids

35.83

Weight (kDa)

9.31

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 132 - 289 1.2e-49 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 74
AccII CGCG 2 cut(s) 612, 710
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 4 cut(s) 186, 198, 404, 882
AclWI GGATC 3 cut(s) 767, 780, 862
AcyI GRCGYC 2 cut(s) 60, 435
AfaI GTAC 3 cut(s) 507, 588, 713
AfiI CCNNNNNNNGG 4 cut(s) 74, 451, 457, 541
AflII CTTAAG 1 cut(s) 911
AgsI TTSAA 4 cut(s) 82, 115, 304, 862
AjiI CACGTC 1 cut(s) 765
AjnI CCWGG 3 cut(s) 627, 639, 927
AleI CACNNNNGTG 1 cut(s) 900
AluBI AGCT 5 cut(s) 313, 490, 559, 649, 940
AluI AGCT 5 cut(s) 313, 490, 559, 649, 940
Alw21I GWGCWC 1 cut(s) 942
Alw26I GTCTC 3 cut(s) 25, 138, 239
AlwI GGATC 3 cut(s) 767, 780, 862
Ama87I CYCGRG 2 cut(s) 414, 650
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 4 cut(s) 264, 429, 625, 925
ApeKI GCWGC 3 cut(s) 183, 401, 559
AspLEI GCGC 3 cut(s) 62, 142, 612
AsuC2I CCSGG 2 cut(s) 98, 452
AsuHPI GGTGA 2 cut(s) 40, 298
AsuII TTCGAA 1 cut(s) 496
AvaI CYCGRG 2 cut(s) 414, 650
BamHI GGATCC 1 cut(s) 772
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 2 cut(s) 657, 942
BbsI GAAGAC 2 cut(s) 159, 249
Bbv12I GWGCWC 1 cut(s) 942
BbvI GCAGC 3 cut(s) 195, 388, 546
BccI CCATC 1 cut(s) 477
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 3 cut(s) 629, 641, 929
BclI TGATCA 2 cut(s) 759, 828
BcnI CCSGG 2 cut(s) 98, 452
BcoDI GTCTC 3 cut(s) 25, 138, 239
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 560
BfoI RGCGCY 2 cut(s) 63, 143
BfrI CTTAAG 1 cut(s) 911
BglII AGATCT 3 cut(s) 223, 386, 787
BisI GCNGC 5 cut(s) 184, 402, 405, 560, 883
BlsI GCNGC 5 cut(s) 185, 403, 406, 561, 884
Bme1390I CCNGG 5 cut(s) 98, 452, 629, 641, 929
BmeT110I CYCGRG 2 cut(s) 414, 650
BmgBI CACGTC 1 cut(s) 765
BmiI GGNNCC 3 cut(s) 61, 702, 774
BmrFI CCNGG 5 cut(s) 98, 452, 629, 641, 929
BpiI GAAGAC 2 cut(s) 159, 249
BpmI CTGGAG 2 cut(s) 377, 507
Bpu14I TTCGAA 1 cut(s) 496
BpuMI CCSGG 2 cut(s) 98, 452
BsaHI GRCGYC 2 cut(s) 60, 435
BsaJI CCNNGG 2 cut(s) 149, 628
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 451, 457, 541
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 594, 900
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 3 cut(s) 629, 641, 929
BseDI CCNNGG 2 cut(s) 149, 628
BseGI GGATG 3 cut(s) 279, 441, 469
BseLI CCNNNNNNNGG 4 cut(s) 74, 451, 457, 541
BseNI ACTGG 2 cut(s) 594, 900
BseRI GAGGAG 9 cut(s) 166, 222, 225, 228, 231, 249, 410, 455, 560
BseXI GCAGC 3 cut(s) 195, 388, 546
BsgI GTGCAG 1 cut(s) 34
Bsh1236I CGCG 2 cut(s) 612, 710
BshFI GGCC 4 cut(s) 266, 431, 627, 927
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 942
BsiHKCI CYCGRG 2 cut(s) 414, 650
BsiSI CCGG 5 cut(s) 97, 392, 432, 451, 607
BslFI GGGAC 4 cut(s) 331, 467, 692, 704
BslI CCNNNNNNNGG 4 cut(s) 74, 451, 457, 541
BsmAI GTCTC 3 cut(s) 25, 138, 239
BsmBI CGTCTC 2 cut(s) 138, 239
BsmFI GGGAC 4 cut(s) 331, 467, 692, 704
BsnI GGCC 4 cut(s) 266, 431, 627, 927
BsoBI CYCGRG 2 cut(s) 414, 650
Bsp119I TTCGAA 1 cut(s) 496
Bsp1286I GDGCHC 2 cut(s) 657, 942
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 8 cut(s) 223, 349, 386, 759, 772, 787, 828, 867
BspACI CCGC 4 cut(s) 186, 198, 404, 882
BspANI GGCC 4 cut(s) 266, 431, 627, 927
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 2 cut(s) 612, 710
BspLI GGNNCC 3 cut(s) 61, 702, 774
BspMAI CTGCAG 1 cut(s) 564
BspPI GGATC 3 cut(s) 767, 780, 862
BspT104I TTCGAA 1 cut(s) 496
BspT107I GGYRCC 1 cut(s) 59
BspTI CTTAAG 1 cut(s) 911
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 2 cut(s) 594, 900
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 149, 628
BssMI GATC 8 cut(s) 223, 349, 386, 759, 772, 787, 828, 867
BssNI GRCGYC 2 cut(s) 60, 435
Bst2UI CCWGG 3 cut(s) 629, 641, 929
Bst4CI ACNGT 1 cut(s) 342
Bst6I CTCTTC 2 cut(s) 452, 791
BstACI GRCGYC 2 cut(s) 60, 435
BstAFI CTTAAG 1 cut(s) 911
BstBI TTCGAA 1 cut(s) 496
BstC8I GCNNGC 2 cut(s) 364, 433
BstF5I GGATG 3 cut(s) 279, 441, 469
BstFNI CGCG 2 cut(s) 612, 710
BstH2I RGCGCY 2 cut(s) 63, 143
BstHHI GCGC 3 cut(s) 62, 142, 612
BstKTI GATC 8 cut(s) 226, 352, 389, 762, 775, 790, 831, 870
BstMAI GTCTC 3 cut(s) 25, 138, 239
BstMBI GATC 8 cut(s) 223, 349, 386, 759, 772, 787, 828, 867
BstMWI GCNNNNNNNGC 3 cut(s) 305, 410, 607
BstNI CCWGG 3 cut(s) 629, 641, 929
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 5 cut(s) 96, 450, 627, 639, 927
BstSFI CTRYAG 1 cut(s) 560
BstUI CGCG 2 cut(s) 612, 710
BstV1I GCAGC 3 cut(s) 195, 388, 546
BstV2I GAAGAC 2 cut(s) 159, 249
BstX2I RGATCY 5 cut(s) 223, 386, 772, 787, 867
BstXI CCANNNNNNTGG 1 cut(s) 374
BstYI RGATCY 5 cut(s) 223, 386, 772, 787, 867
BsuRI GGCC 4 cut(s) 266, 431, 627, 927
BtrI CACGTC 1 cut(s) 765
BtsCI GGATG 3 cut(s) 279, 441, 469
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 22, 907
Cac8I GCNNGC 2 cut(s) 364, 433
CfoI GCGC 3 cut(s) 62, 142, 612
Cfr10I RCCGGY 1 cut(s) 431
CseI GACGC 2 cut(s) 424, 824
Csp6I GTAC 3 cut(s) 506, 587, 712
CviAII CATG 3 cut(s) 69, 602, 687
CviQI GTAC 3 cut(s) 506, 587, 712
DinI GGCGCC 1 cut(s) 61
DpnI GATC 8 cut(s) 225, 351, 388, 761, 774, 789, 830, 869
DpnII GATC 8 cut(s) 223, 349, 386, 759, 772, 787, 828, 867
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 452, 791
EarI CTCTTC 2 cut(s) 452, 791
EciI GGCGGA 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 940
Eco147I AGGCCT 1 cut(s) 927
Eco24I GRGCYC 2 cut(s) 657, 942
Eco32I GATATC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 940
Eco88I CYCGRG 2 cut(s) 414, 650
EcoICRI GAGCTC 1 cut(s) 940
EcoRII CCWGG 3 cut(s) 627, 639, 927
EcoRV GATATC 1 cut(s) 252
EcoT38I GRGCYC 2 cut(s) 657, 942
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
Esp3I CGTCTC 2 cut(s) 138, 239
FaeI CATG 3 cut(s) 72, 605, 690
FalI AAGNNNNNCTT 2 cut(s) 788, 820
FaqI GGGAC 4 cut(s) 331, 467, 692, 704
FatI CATG 3 cut(s) 68, 601, 686
FbaI TGATCA 2 cut(s) 759, 828
Fnu4HI GCNGC 5 cut(s) 184, 402, 405, 560, 883
FokI GGATG 3 cut(s) 286, 428, 456
FriOI GRGCYC 2 cut(s) 657, 942
Fsp4HI GCNGC 5 cut(s) 184, 402, 405, 560, 883
FspBI CTAG 1 cut(s) 491
GlaI GCGC 3 cut(s) 61, 141, 611
GluI GCNGC 5 cut(s) 184, 402, 405, 560, 883
GsuI CTGGAG 2 cut(s) 377, 507
HaeII RGCGCY 2 cut(s) 63, 143
HaeIII GGCC 4 cut(s) 266, 431, 627, 927
HapII CCGG 5 cut(s) 97, 392, 432, 451, 607
HgaI GACGC 2 cut(s) 424, 824
HhaI GCGC 3 cut(s) 62, 142, 612
Hin1I GRCGYC 2 cut(s) 60, 435
Hin1II CATG 3 cut(s) 72, 605, 690
Hin6I GCGC 3 cut(s) 60, 140, 610
HinP1I GCGC 3 cut(s) 60, 140, 610
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 5 cut(s) 78, 145, 520, 916, 947
HpaII CCGG 5 cut(s) 97, 392, 432, 451, 607
HphI GGTGA 2 cut(s) 40, 298
Hpy188I TCNGA 5 cut(s) 150, 262, 349, 777, 792
Hpy188III TCNNGA 7 cut(s) 356, 392, 422, 524, 757, 811, 871
Hpy99I CGWCG 2 cut(s) 428, 947
HpyAV CCTTC 3 cut(s) 298, 317, 565
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 2 cut(s) 693, 764
HpyCH4V TGCA 3 cut(s) 15, 125, 562
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 410, 607
HpySE526I ACGT 2 cut(s) 693, 764
Hsp92I GRCGYC 2 cut(s) 60, 435
Hsp92II CATG 3 cut(s) 72, 605, 690
HspAI GCGC 3 cut(s) 60, 140, 610
KasI GGCGCC 1 cut(s) 59
Kpn2I TCCGGA 1 cut(s) 391
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
Ksp22I TGATCA 2 cut(s) 759, 828
Kzo9I GATC 8 cut(s) 223, 349, 386, 759, 772, 787, 828, 867
LmnI GCTCC 2 cut(s) 218, 487
Lsp1109I GCAGC 3 cut(s) 195, 388, 546
MaeI CTAG 1 cut(s) 491
MaeII ACGT 2 cut(s) 693, 764
MalI GATC 8 cut(s) 225, 351, 388, 761, 774, 789, 830, 869
MboI GATC 8 cut(s) 223, 349, 386, 759, 772, 787, 828, 867
MflI RGATCY 5 cut(s) 223, 386, 772, 787, 867
MhlI GDGCHC 2 cut(s) 657, 942
MluCI AATT 2 cut(s) 579, 841
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 2 cut(s) 514, 941
MmeI TCCRAC 1 cut(s) 800
MroI TCCGGA 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 431
MseI TTAA 3 cut(s) 551, 912, 964
MslI CAYNNNNRTG 2 cut(s) 600, 900
MspA1I CMGCKG 2 cut(s) 186, 559
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 5 cut(s) 97, 392, 432, 451, 607
MspR9I CCNGG 5 cut(s) 98, 452, 629, 641, 929
MvaI CCWGG 3 cut(s) 629, 641, 929
MvnI CGCG 2 cut(s) 612, 710
MwoI GCNNNNNNNGC 3 cut(s) 305, 410, 607
NaeI GCCGGC 1 cut(s) 433
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 2 cut(s) 98, 452
NdeII GATC 8 cut(s) 223, 349, 386, 759, 772, 787, 828, 867
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 3 cut(s) 72, 605, 690
NlaIV GGNNCC 3 cut(s) 61, 702, 774
NspI RCATGY 1 cut(s) 605
NspV TTCGAA 1 cut(s) 496
OliI CACNNNNGTG 1 cut(s) 900
PceI AGGCCT 1 cut(s) 927
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PdiI GCCGGC 1 cut(s) 433
PfeI GAWTC 3 cut(s) 78, 145, 916
PflMI CCANNNNNTGG 1 cut(s) 74
PfoI TCCNGGA 1 cut(s) 450
PkrI GCNGC 5 cut(s) 185, 403, 406, 561, 884
PleI GAGTC 2 cut(s) 514, 941
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 2 cut(s) 514, 941
Psp124BI GAGCTC 1 cut(s) 942
Psp6I CCWGG 3 cut(s) 627, 639, 927
PspGI CCWGG 3 cut(s) 627, 639, 927
PspN4I GGNNCC 3 cut(s) 61, 702, 774
PstI CTGCAG 1 cut(s) 564
PsuI RGATCY 5 cut(s) 223, 386, 772, 787, 867
PvuII CAGCTG 1 cut(s) 559
RsaI GTAC 3 cut(s) 507, 588, 713
RsaNI GTAC 3 cut(s) 506, 587, 712
RseI CAYNNNNRTG 2 cut(s) 600, 900
SacI GAGCTC 1 cut(s) 942
SaqAI TTAA 3 cut(s) 551, 912, 964
SatI GCNGC 5 cut(s) 184, 402, 405, 560, 883
Sau3AI GATC 8 cut(s) 223, 349, 386, 759, 772, 787, 828, 867
SchI GAGTC 2 cut(s) 514, 941
ScrFI CCNGG 5 cut(s) 98, 452, 629, 641, 929
SduI GDGCHC 2 cut(s) 657, 942
SfcI CTRYAG 1 cut(s) 560
SfoI GGCGCC 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 496
SmiMI CAYNNNNRTG 2 cut(s) 600, 900
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
Sse9I AATT 2 cut(s) 579, 841
SseBI AGGCCT 1 cut(s) 927
SsiI CCGC 4 cut(s) 186, 198, 404, 882
SspDI GGCGCC 1 cut(s) 59
SspI AATATT 1 cut(s) 118
SspMI CTAG 1 cut(s) 491
SstI GAGCTC 1 cut(s) 942
StuI AGGCCT 1 cut(s) 927
StyD4I CCNGG 5 cut(s) 96, 450, 627, 639, 927
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 2 cut(s) 696, 767
TaqI TCGA 4 cut(s) 423, 496, 852, 942
TasI AATT 2 cut(s) 579, 841
TatI WGTACW 1 cut(s) 586
TauI GCSGC 2 cut(s) 407, 885
TfiI GAWTC 3 cut(s) 78, 145, 916
Tru1I TTAA 3 cut(s) 551, 912, 964
Tru9I TTAA 3 cut(s) 551, 912, 964
TscAI CASTG 2 cut(s) 22, 907
TseI GCWGC 3 cut(s) 183, 401, 559
TspDTI ATGAA 1 cut(s) 809
TspRI CASTG 2 cut(s) 22, 907
Van91I CCANNNNNTGG 1 cut(s) 74
Vha464I CTTAAG 1 cut(s) 911
XceI RCATGY 1 cut(s) 605
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.