AT1G58050

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007888
Physical Location & Seq
Reverse (-)
21478590 .. 21487839
9250 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G58050.1

Sequence Viewer

Length: 4254 bp
ATGGCTCCGACGAAGAAACCTCAGAAGAACAAACAAAGCAAAAACGAAATAGCTTCTTCTTTAATACCTAATTCAGGCCATAAGAAACCATCTAAAGCCCCAAAGCTTTTAATTTCGCCGGAAAACGAAGACCGTTTACGACGGCTCTTGCTCAACTTTCGCCGGACGCCTTCTCCGGTCACGGCGACGCTATCTGTTACTCAGAAGAGAAAGAAACTCAATAATCTCTACGAGAATCTCTCTTGCGAAGGGTTTTTAGATAATCAGATTGAACTCGTTCTCTCCTCTCTTAGGGATGGAGCAACGCTTGAGACTGCTCTTGATTGGCTTTGCTTGAACTTGCCGAGCCATGAATTGCCAGTGAACTTTTCTAACGGGGCTTCTCGTTTTCCTTCCACAGGACGTTCTGTGGCGGTGATATCGAAATCAAAGAAAGATTGGAATGTGTCTGCTGAATCATCAGTCCAAGAGGTTAAGGAGGTGCCTGAATCTGAAGTTTTAGTTCGAGTGAAGAGCAAGCGAGATGAAGAAGAAGAAGATAGTCTTAGTTCTTGCCAACCATCTCAAGCTGATTGGATCCATCAGTATATGAAAAGACTGGAAGAGGAGGAATTGGAATCTTCCGACGATGAGAGAGATAAGGTATCTGGTCCAAGGTCTTTCGAAGTGATTGCCAAAGAATATTGTGTAGAAAGATATAATGCTATAAAAGCAAAGAGGAAGGGTGATAAAAGTGGACAGAGCCAAGCAGGCCTAGCCATTTGCAAGCTCAAGGAAGAGATGAATGCTCTTGGGCCGTCAGAAGCAATATTGGAATCTGAGTTTCAGCGTGATCGTCAAGATTGTGAAGGCGCTAGAGAAAAAGAAGTAGCCTCCTCTGTGCTTGATGACGTTCATGAGTCAGTAAATGCTGATGCTTTCTTTTTTCAGCTTTTTGATGACTTGACCTTGGATACAAATCCTGTTGGGAGCTGTAAATCTGAGGAACAAGAATCAGTTGTCTCGAATGATAGTTTAGATGACTTGGACCTTGGCGATTTGTTCGACGAAGATGTTCCGCCTTATGTACCTTCTCCCCATGAACTCCTGGAATTACAGAAGAAGGAAATTATGAGAGAACTATGCAATGAGAAACATCTTACGAAACTAAACGGTATCTGGAAGAAGGGTGAAGCGCAAAAGATCCCGAAGGCATTGCTCCATCAGTTGTGTCAAAGGTCAGGGTGGATAGCACCAAAGTTCAATAAAGTGACTGGGGAAGGAAGAAACTTCTCATATACTACAAGTGTTATGCGTAAATCCAGTGGATTTGGTAAAAGCAGACAAGCCGGGGGTCTGGTAACTATTCAACTTCCTCATCAAGTTGAGGATTTTGAATCCATCCAGGATGCACAAAACAGAGTTGCGGCATTTGCTCTCCATAAGCTATTCTCTGATTTGCCTGTTCACTTTGCAATTACTGAGCCTTATGCCTCTCTAGTTTTGATCTGGAAACAAGAGGAATCATTAGGTATAACAAGCAGAGAAGAGCAAAGAAGAGAAAAATTTGTTGAAAGCTTGCTTGAGGCAGACAATTTCAGCTTGACTACTACTTCGAGAGGCATACACAGTGCACTTCCAATGGTAGACTCCTGTGTCAAAGAGAATGATGACTTGGATGTTGTCAAATCTAACCACAGAGCCAGAAGAAACTCTTCCATGGCAGCTGAATGTTCTTCTCTCAAACAAAAACAAGAAAACAAGAAGAAGATGCAGAAGTACAAGGACATGTTGAAAACTAGAGCTGCTCTTCCTATATCAGAAGTGAAAAAGGACATACTGCAGAAGCTGAAAGAAAAAGATGTCTTGGTGGTATGCGGAGAAACTGGTTCCGGAAAGACTACACAGGTTCCTCAATTTATACTGGATGATATGATCGATTCAGGGCATGGTGGATACTGTAATATTATATGCACACAACCTCGGGCTATCACTGTTGCTCAAAGAGTTGCTGATGAGCGCTGTGAACCTCCTCCAGGCTTCGACAACTCTGTGGTTGCTTATCAAGTTCGTCATCAAAATGCAAGGAGTGATAAGACAAGGCTGCTGTTTTGTACTACTGGAATCCTACTGAGAAAACTTGTGGGGGATACGACCTTGAAAGATGTTACACATATTATAGTCGATGAAGTGCATGAGCGGTCTCTTATGGGTGATTTTCTTCTCATTATTTTGAAGAGCCTGATTGAGAAGCAATCCTGGGATAACGCATTACCTAAACTAAAAGTTATCCTTATGTCCGCTACTGTTGATGCACATCAGTTCTCAAGATATTTTGGTCAATGTCCTATAATTACTGCTCAAGGACGAACTCACCCGGTTACCACTTACTTTCTGGAGGACATTTATGAGAGGACTAAGTACCTTCTGGCTTCAGATTCACCTGCTGCTCTAAGTTCTGATACATCCATTACAGACAAGCTTGGTTCTGTAAACGTCCCTAGGGGGAAAAAGAACCTTATGTTGGCTGGCTGGGGAGACAGTTATTTGGTTTCAGAGGACAGTCTTAACACGTCTTACGATTCCATTAAATATATTGCAAGTGCTGTTGTTGACTATGATCTTCTTGAAGAGTTGATATGTCACATTGATGACACTTGTGAGGAAGGAGCCATTCTTGTGTTTTTGCCTGGAATGTCAGAAATCAACATGCTACTCAATAGGTTAGCTGCTTCCTATCGTTTTCGTGGAGCTTCTGGAGATTGGCTTCTTCCTCTACATTCTTCTATTGCATCTACAGAACAGAAAAAGGTGTTTCTGCGCCCCCCTAAAGGCATACGGAAGGTTATCATAGCTACAAATATTGCCGAGACCAGTATAACAATTGAGGATGTGGTTTATGTGATTGATAGTGGAAAACACAAGGAAAATCGCTATAACCCACATAAGAAATTGTCAAGCATGGTGGAAGATTGGGTATCCAAAGCAAATGCAAGACAAAGAATGGGAAGAGCTGGACGTGTCAAACCTGGACATTGCTTTTCATTATACACTCGCCATAGGTTCGAGAAGCTCATGCGTCCCTATCAGGTTCCTGAGATGCTGCGGGTGCCTTTAGTAGAGCTGTGTTTACATATCAAATTACTTGGCTTGGGTCAAATTAAGCCGTTTCTGTCCAAGGCTTTGGAACCTCCGAGTGAAAGTGCTATAAACTCTGCAATTTTATTGTTGCACAAGGTCGGCGCTCTTGAAGGGGACGAAGAGTTGACACCACTAGGACATCATTTAGCAAAACTTCCTGTTGATTTGTTAATTGGAAAGATGCTGTTATATGGTGGCATTTTTGGTTGCTTATCACCTATTCTCTCGATTGCTGCTTTCTTGAGCTGCTGCAAATCGCCGTTTGTATATGCCAAAGATGAGCAAAATGTGGACCGGGTGAAACTTGCTCTTTTGTCTGATAAGCTGGAGAGTTCGAGTAATTTAAACAACAATGATAGACAATCTGATCACCTCCTTATGGTGGTTGCATATGAAAAATGGGTGAGGATACTGCATGAGCAAGGATTTAAAGCTGCAGAGAGTTTCTGTGAGTCAAAGTTTTTGAATAGCTCAGTGATGCGGATGATGAGAGAGAGGAGAGTAGAGTTTGGCATGTTGCTTGCCGACATCGGGCTCATCAATCTTCCTAAAGGCAAGGGGAGAAGGAAGGAGAATTTTGATGTTTGGTTTTCTGACAAAACTCAACCTTTCAATATGTATTCACAAGAACCTGAAGTTGTTAAGGCTATATTATGCGCGGGACTATGTCCTAATATTGCAGAGGGATTAGTTAACCGTTTAACAAAGCCAGCGGAAGAAACACAGCGTTATGCAGTATGGCATGATGGCAAAAGAGAAGTCCATATCCATCGAAACTCTATTAACAAAAACTGCAAAGCGTTTCAATACCCTTTCATTGTGTTCCTTGAAAAGCTCGAAACAAAGAAAGTGGTATATCTGCAAGATACAACTGTTGTTTCTCCGTTCTCCATTCTACTTTTTGGCGGCTCAATCAATGTTCATCATCAGAGTGGATCAGTTACCATTGATGGGTGGTTAAAGCTAACAGCACCAGCACAAACTGCCGTTTTGTTCAAAGAGCTCCGGTTAACCCTTCATTCCATTCTCAAAGATCTCATCCGAAAACCCGAGAAATCAGGTATTGTCCACAACGAAGTCGTCAAATCTATGGTCCATCTTCTTATAGAAGAAGGCAAACCGCAACACACATGA

Protein Analysis

1417

Amino Acids

159.34

Weight (kDa)

7.37

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_DHX29 PF24899 72 - 124 6e-16 ATP-dependent RNA helicase DHX29, UBA domain
DEAD PF00270 604 - 764 1.1e-10 DEAD/DEAH box helicase
Helicase_C PF00271 871 - 1001 9.8e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1066 - 1094 2.4e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1098 - 1166 1e-09 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1254 - 1345 2.2e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1354 - 1408 3.7e-14 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2431
AasI GACNNNNNNGTC 1 cut(s) 1634
Acc36I ACCTGC 1 cut(s) 2431
AccB1I GGYRCC 2 cut(s) 481, 3088
AccBSI CCGCTC 1 cut(s) 2179
AccI GTMKAC 1 cut(s) 1626
AccII CGCG 1 cut(s) 3779
AccIII TCCGGA 1 cut(s) 1871
AclWI GGATC 4 cut(s) 571, 584, 1177, 4063
AcsI RAATTY 2 cut(s) 1544, 3694
AcuI CTGAAG 3 cut(s) 513, 2397, 3774
AcyI GRCGYC 1 cut(s) 167
AfaI GTAC 4 cut(s) 1068, 1760, 2095, 2402
AfeI AGCGCT 1 cut(s) 2000
AflIII ACRYGT 3 cut(s) 1767, 2550, 2998
AjiI CACGTC 2 cut(s) 2553, 2999
AjnI CCWGG 6 cut(s) 1086, 1383, 2014, 2237, 2668, 3007
AjuI GAANNNNNNNTTGG 4 cut(s) 1637, 1669, 2486, 2518
Alw21I GWGCWC 2 cut(s) 1615, 4125
Alw26I GTCTC 5 cut(s) 305, 1006, 2187, 2511, 2843
Alw44I GTGCAC 1 cut(s) 1611
AlwI GGATC 4 cut(s) 571, 584, 1177, 4063
AlwNI CAGNNNCTG 1 cut(s) 1828
Ama87I CYCGRG 2 cut(s) 1962, 4169
Aor13HI TCCGGA 1 cut(s) 1871
Aor51HI AGCGCT 1 cut(s) 2000
AoxI GGCC 3 cut(s) 76, 751, 794
ApaLI GTGCAC 1 cut(s) 1611
ApoI RAATTY 2 cut(s) 1544, 3694
ArsI GACNNNNNNTTYG 6 cut(s) 1576, 1608, 3864, 3896, 4197, 4229
Asp700I GAANNNNTTC 3 cut(s) 276, 1053, 1693
AspA2I CCTAGG 1 cut(s) 2480
AspLEI GCGC 6 cut(s) 854, 1177, 2001, 2802, 3224, 3779
AspS9I GGNCC 5 cut(s) 650, 794, 1027, 3412, 4213
AsuC2I CCSGG 3 cut(s) 1330, 2357, 3416
AsuII TTCGAA 1 cut(s) 663
AvaI CYCGRG 2 cut(s) 1962, 4169
AvaII GGWCC 4 cut(s) 650, 1027, 3412, 4213
AvrII CCTAGG 1 cut(s) 2480
BaeGI GKGCMC 1 cut(s) 1615
BaeI ACNNNNGTAYC 2 cut(s) 3978, 4011
BamHI GGATCC 1 cut(s) 576
BanI GGYRCC 2 cut(s) 481, 3088
BanII GRGCYC 2 cut(s) 3657, 4125
BarI GAAGNNNNNNTAC 2 cut(s) 120, 152
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 2 cut(s) 1615, 4125
BceAI ACGGC 6 cut(s) 158, 198, 781, 3130, 3364, 4091
BcgI CGANNNNNNTGC 4 cut(s) 653, 687, 1177, 1211
BciT130I CCWGG 6 cut(s) 1088, 1385, 2016, 2239, 2670, 3009
BciVI GTATCC 5 cut(s) 946, 1928, 2122, 2968, 3522
BclI TGATCA 1 cut(s) 3487
BcnI CCSGG 3 cut(s) 1330, 2357, 3416
BcoDI GTCTC 5 cut(s) 305, 1006, 2187, 2511, 2843
BfaI CTAG 6 cut(s) 755, 855, 1478, 1779, 2481, 3254
BfmI CTRYAG 3 cut(s) 1820, 2775, 3555
BfoI RGCGCY 3 cut(s) 855, 2002, 3225
BfuAI ACCTGC 1 cut(s) 2431
BfuI GTATCC 5 cut(s) 946, 1928, 2122, 2968, 3522
BglI GCCNNNNNGGC 1 cut(s) 750
BglII AGATCT 1 cut(s) 4153
BlnI CCTAGG 1 cut(s) 2480
Bme1390I CCNGG 9 cut(s) 1088, 1330, 1385, 2016, 2239, 2357, 2670, 3009, 3416
Bme18I GGWCC 4 cut(s) 650, 1027, 3412, 4213
BmeT110I CYCGRG 2 cut(s) 1962, 4169
BmgBI CACGTC 2 cut(s) 2553, 2999
BmgT120I GGNCC 5 cut(s) 650, 794, 1027, 3412, 4213
BmiI GGNNCC 9 cut(s) 6, 483, 578, 1870, 1890, 2650, 3072, 3090, 3168
BmrFI CCNGG 9 cut(s) 1088, 1330, 1385, 2016, 2239, 2357, 2670, 3009, 3416
BmrI ACTGGG 1 cut(s) 1263
BmsI GCATC 8 cut(s) 904, 1378, 1740, 2281, 2780, 3069, 3291, 3588
BmuI ACTGGG 1 cut(s) 1263
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpmI CTGGAG 4 cut(s) 1998, 2396, 2757, 3467
Bpu14I TTCGAA 1 cut(s) 663
BpuEI CTTGAG 7 cut(s) 329, 549, 755, 1583, 2290, 2325, 3382
BpuMI CCSGG 3 cut(s) 1330, 2357, 3416
Bsa29I ATCGAT 1 cut(s) 1917
BsaBI GATNNNNATC 2 cut(s) 957, 2295
BsaHI GRCGYC 1 cut(s) 167
BsaI GGTCTC 2 cut(s) 2187, 2843
BsaJI CCNNGG 9 cut(s) 653, 948, 1030, 1329, 1698, 1961, 2238, 2480, 3156
BsaWI WCCGGW 3 cut(s) 175, 1871, 4125
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bse1I ACTGG 8 cut(s) 359, 603, 1258, 1302, 1870, 1908, 2104, 2853
Bse3DI GCAATG 3 cut(s) 1132, 1193, 3013
Bse8I GATNNNNATC 2 cut(s) 957, 2295
BseAI TCCGGA 1 cut(s) 1871
BseBI CCWGG 6 cut(s) 1088, 1385, 2016, 2239, 2670, 3009
BseCI ATCGAT 1 cut(s) 1917
BseDI CCNNGG 9 cut(s) 653, 948, 1030, 1329, 1698, 1961, 2238, 2480, 3156
BseGI GGATG 9 cut(s) 301, 1380, 1393, 1663, 1912, 2444, 2875, 3609, 4158
BseJI GATNNNNATC 2 cut(s) 957, 2295
BseMI GCAATG 3 cut(s) 1132, 1193, 3013
BseMII CTCAG 8 cut(s) 35, 215, 810, 972, 1452, 2102, 3066, 3606
BseNI ACTGG 8 cut(s) 359, 603, 1258, 1302, 1870, 1908, 2104, 2853
BseRI GAGGAG 5 cut(s) 274, 620, 865, 2001, 3631
BseSI GKGCMC 1 cut(s) 1615
BseYI CCCAGC 1 cut(s) 2511
Bsh1236I CGCG 1 cut(s) 3779
BshFI GGCC 3 cut(s) 78, 753, 796
BshNI GGYRCC 2 cut(s) 481, 3088
BshVI ATCGAT 1 cut(s) 1917
BsiHKAI GWGCWC 2 cut(s) 1615, 4125
BsiHKCI CYCGRG 2 cut(s) 1962, 4169
BsiSI CCGG 8 cut(s) 119, 163, 176, 1329, 1872, 2357, 3415, 4126
BslFI GGGAC 4 cut(s) 2462, 3045, 3248, 3795
BsmAI GTCTC 5 cut(s) 305, 1006, 2187, 2511, 2843
BsmFI GGGAC 4 cut(s) 2462, 3045, 3248, 3795
BsmI GAATGC 1 cut(s) 790
BsnI GGCC 3 cut(s) 78, 753, 796
Bso31I GGTCTC 2 cut(s) 2187, 2843
BsoBI CYCGRG 2 cut(s) 1962, 4169
Bsp119I TTCGAA 1 cut(s) 663
Bsp1286I GDGCHC 3 cut(s) 1615, 3657, 4125
Bsp13I TCCGGA 1 cut(s) 1871
Bsp143I GATC 9 cut(s) 576, 832, 1182, 1485, 1914, 2599, 3487, 4055, 4153
Bsp19I CCATGG 1 cut(s) 1698
BspANI GGCC 3 cut(s) 78, 753, 796
BspCNI CTCAG 8 cut(s) 34, 214, 811, 973, 1453, 2103, 3067, 3605
BspDI ATCGAT 1 cut(s) 1917
BspEI TCCGGA 1 cut(s) 1871
BspFNI CGCG 1 cut(s) 3779
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 9 cut(s) 6, 483, 578, 1870, 1890, 2650, 3072, 3090, 3168
BspMAI CTGCAG 2 cut(s) 1824, 3559
BspMI ACCTGC 1 cut(s) 2431
BspPI GGATC 4 cut(s) 571, 584, 1177, 4063
BspQI GCTCTTC 5 cut(s) 506, 1521, 1794, 2210, 2983
BspT104I TTCGAA 1 cut(s) 663
BspT107I GGYRCC 2 cut(s) 481, 3088
BspTNI GGTCTC 2 cut(s) 2187, 2843
BsrBI CCGCTC 1 cut(s) 2179
BsrDI GCAATG 3 cut(s) 1132, 1193, 3013
BsrI ACTGG 8 cut(s) 359, 603, 1258, 1302, 1870, 1908, 2104, 2853
BssECI CCNNGG 9 cut(s) 653, 948, 1030, 1329, 1698, 1961, 2238, 2480, 3156
BssMI GATC 9 cut(s) 576, 832, 1182, 1485, 1914, 2599, 3487, 4055, 4153
BssNI GRCGYC 1 cut(s) 167
BssT1I CCWWGG 6 cut(s) 653, 948, 1030, 1698, 2480, 3156
Bst2UI CCWGG 6 cut(s) 1088, 1385, 2016, 2239, 2670, 3009
BstACI GRCGYC 1 cut(s) 167
BstAPI GCANNNNNTGC 1 cut(s) 4103
BstBI TTCGAA 1 cut(s) 663
BstC8I GCNNGC 7 cut(s) 518, 751, 767, 1559, 2509, 3642, 3831
BstDSI CCRYGG 1 cut(s) 1698
BstEII GGTNACC 1 cut(s) 2359
BstENI CCTNNNNNAGG 4 cut(s) 72, 289, 396, 2013
BstF5I GGATG 9 cut(s) 301, 1380, 1393, 1663, 1912, 2444, 2875, 3609, 4158
BstFNI CGCG 1 cut(s) 3779
BstH2I RGCGCY 3 cut(s) 855, 2002, 3225
BstHHI GCGC 6 cut(s) 854, 1177, 2001, 2802, 3224, 3779
BstKTI GATC 9 cut(s) 579, 835, 1185, 1488, 1917, 2602, 3490, 4058, 4156
BstMAI GTCTC 5 cut(s) 305, 1006, 2187, 2511, 2843
BstMBI GATC 9 cut(s) 576, 832, 1182, 1485, 1914, 2599, 3487, 4055, 4153
BstMWI GCNNNNNNNGC 6 cut(s) 710, 750, 755, 1412, 3088, 4103
BstNI CCWGG 6 cut(s) 1088, 1385, 2016, 2239, 2670, 3009
BstNSI RCATGY 3 cut(s) 1771, 2692, 3637
BstPI GGTNACC 1 cut(s) 2359
BstSCI CCNGG 9 cut(s) 1086, 1328, 1383, 2014, 2237, 2355, 2668, 3007, 3414
BstSFI CTRYAG 3 cut(s) 1820, 2775, 3555
BstSLI GKGCMC 1 cut(s) 1615
BstUI CGCG 1 cut(s) 3779
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 3 cut(s) 576, 1182, 4153
BstXI CCANNNNNNTGG 1 cut(s) 3163
BstYI RGATCY 3 cut(s) 576, 1182, 4153
Bsu15I ATCGAT 1 cut(s) 1917
BsuI GTATCC 5 cut(s) 946, 1928, 2122, 2968, 3522
BsuRI GGCC 3 cut(s) 78, 753, 796
BsuTUI ATCGAT 1 cut(s) 1917
BtgI CCRYGG 1 cut(s) 1698
BtrI CACGTC 2 cut(s) 2553, 2999
BtsCI GGATG 9 cut(s) 301, 1380, 1393, 1663, 1912, 2444, 2875, 3609, 4158
BtsIMutI CAGTG 5 cut(s) 366, 1309, 1615, 1971, 3600
BveI ACCTGC 1 cut(s) 2431
Cac8I GCNNGC 7 cut(s) 518, 751, 767, 1559, 2509, 3642, 3831
CaiI CAGNNNCTG 1 cut(s) 1828
CciI TCATGA 1 cut(s) 895
CfoI GCGC 6 cut(s) 854, 1177, 2001, 2802, 3224, 3779
Cfr13I GGNCC 5 cut(s) 650, 794, 1027, 3412, 4213
ClaI ATCGAT 1 cut(s) 1917
CseI GACGC 3 cut(s) 175, 196, 3047
Csp6I GTAC 4 cut(s) 1067, 1759, 2094, 2401
CspCI CAANNNNNGTGG 4 cut(s) 2925, 2960, 3951, 3986
CviQI GTAC 4 cut(s) 1067, 1759, 2094, 2401
DpnI GATC 9 cut(s) 578, 834, 1184, 1487, 1916, 2601, 3489, 4057, 4155
DpnII GATC 9 cut(s) 576, 832, 1182, 1485, 1914, 2599, 3487, 4055, 4153
DraI TTTAAA 2 cut(s) 3465, 3550
DrdI GACNNNNNNGTC 1 cut(s) 1634
DseDI GACNNNNNNGTC 1 cut(s) 1634
EciI GGCGGA 1 cut(s) 1047
Ecl136II GAGCTC 1 cut(s) 4123
Eco130I CCWWGG 6 cut(s) 653, 948, 1030, 1698, 2480, 3156
Eco147I AGGCCT 1 cut(s) 753
Eco24I GRGCYC 2 cut(s) 3657, 4125
Eco31I GGTCTC 2 cut(s) 2187, 2843
Eco32I GATATC 1 cut(s) 420
Eco47I GGWCC 4 cut(s) 650, 1027, 3412, 4213
Eco47III AGCGCT 1 cut(s) 2000
Eco53kI GAGCTC 1 cut(s) 4123
Eco57I CTGAAG 3 cut(s) 513, 2397, 3774
Eco88I CYCGRG 2 cut(s) 1962, 4169
Eco91I GGTNACC 1 cut(s) 2359
EcoICRI GAGCTC 1 cut(s) 4123
EcoNI CCTNNNNNAGG 4 cut(s) 72, 289, 396, 2013
EcoO65I GGTNACC 1 cut(s) 2359
EcoRII CCWGG 6 cut(s) 1086, 1383, 2014, 2237, 2668, 3007
EcoRV GATATC 1 cut(s) 420
EcoT14I CCWWGG 6 cut(s) 653, 948, 1030, 1698, 2480, 3156
EcoT38I GRGCYC 2 cut(s) 3657, 4125
ErhI CCWWGG 6 cut(s) 653, 948, 1030, 1698, 2480, 3156
FalI AAGNNNNNCTT 6 cut(s) 528, 560, 1678, 1710, 2256, 2288
FaqI GGGAC 4 cut(s) 2462, 3045, 3248, 3795
FauI CCCGC 2 cut(s) 3078, 3772
FauNDI CATATG 1 cut(s) 3511
FbaI TGATCA 1 cut(s) 3487
FblI GTMKAC 1 cut(s) 1626
FokI GGATG 9 cut(s) 308, 1367, 1400, 1670, 1919, 2431, 2882, 3616, 4145
FriOI GRGCYC 2 cut(s) 3657, 4125
FspBI CTAG 6 cut(s) 755, 855, 1478, 1779, 2481, 3254
GlaI GCGC 6 cut(s) 853, 1176, 2000, 2801, 3223, 3778
GsaI CCCAGC 1 cut(s) 2515
GsuI CTGGAG 4 cut(s) 1998, 2396, 2757, 3467
HaeII RGCGCY 3 cut(s) 855, 2002, 3225
HaeIII GGCC 3 cut(s) 78, 753, 796
HapII CCGG 8 cut(s) 119, 163, 176, 1329, 1872, 2357, 3415, 4126
HgaI GACGC 3 cut(s) 175, 196, 3047
HhaI GCGC 6 cut(s) 854, 1177, 2001, 2802, 3224, 3779
Hin1I GRCGYC 1 cut(s) 167
Hin6I GCGC 6 cut(s) 852, 1175, 1999, 2800, 3222, 3777
HinP1I GCGC 6 cut(s) 852, 1175, 1999, 2800, 3222, 3777
HincII GTYRAC 4 cut(s) 2593, 3246, 3814, 4131
HindII GTYRAC 4 cut(s) 2593, 3246, 3814, 4131
HindIII AAGCTT 3 cut(s) 104, 1555, 2459
HpaI GTTAAC 2 cut(s) 3814, 4131
HpaII CCGG 8 cut(s) 119, 163, 176, 1329, 1872, 2357, 3415, 4126
Hpy99I CGWCG 5 cut(s) 13, 144, 190, 629, 1049
HpyCH4IV ACGT 5 cut(s) 403, 891, 2475, 2552, 2998
HpyF10VI GCNNNNNNNGC 6 cut(s) 710, 750, 755, 1412, 3088, 4103
HpySE526I ACGT 5 cut(s) 403, 891, 2475, 2552, 2998
Hsp92I GRCGYC 1 cut(s) 167
HspAI GCGC 6 cut(s) 852, 1175, 1999, 2800, 3222, 3777
Kpn2I TCCGGA 1 cut(s) 1871
Ksp22I TGATCA 1 cut(s) 3487
KspAI GTTAAC 2 cut(s) 3814, 4131
Kzo9I GATC 9 cut(s) 576, 832, 1182, 1485, 1914, 2599, 3487, 4055, 4153
LguI GCTCTTC 5 cut(s) 506, 1521, 1794, 2210, 2983
LmnI GCTCC 7 cut(s) 10, 299, 969, 1203, 2648, 2729, 4128
LweI GCATC 8 cut(s) 904, 1378, 1740, 2281, 2780, 3069, 3291, 3588
MaeI CTAG 6 cut(s) 755, 855, 1478, 1779, 2481, 3254
MaeII ACGT 5 cut(s) 403, 891, 2475, 2552, 2998
MaeIII GTNAC 8 cut(s) 178, 196, 1249, 1339, 2146, 2359, 2621, 4060
MalI GATC 9 cut(s) 578, 834, 1184, 1487, 1916, 2601, 3489, 4057, 4155
MbiI CCGCTC 1 cut(s) 2179
MboI GATC 9 cut(s) 576, 832, 1182, 1485, 1914, 2599, 3487, 4055, 4153
MfeI CAATTG 1 cut(s) 2862
MflI RGATCY 3 cut(s) 576, 1182, 4153
MhlI GDGCHC 3 cut(s) 1615, 3657, 4125
MlyI GAGTC 3 cut(s) 908, 1622, 3581
MmeI TCCRAC 2 cut(s) 32, 648
MroI TCCGGA 1 cut(s) 1871
MroXI GAANNNNTTC 3 cut(s) 276, 1053, 1693
MslI CAYNNNNRTG 4 cut(s) 2058, 2628, 2657, 4050
MspA1I CMGCKG 2 cut(s) 1706, 3833
MspI CCGG 8 cut(s) 119, 163, 176, 1329, 1872, 2357, 3415, 4126
MspR9I CCNGG 9 cut(s) 1088, 1330, 1385, 2016, 2239, 2357, 2670, 3009, 3416
MunI CAATTG 1 cut(s) 2862
Mva1269I GAATGC 1 cut(s) 790
MvaI CCWGG 6 cut(s) 1088, 1385, 2016, 2239, 2670, 3009
MvnI CGCG 1 cut(s) 3779
MwoI GCNNNNNNNGC 6 cut(s) 710, 750, 755, 1412, 3088, 4103
NciI CCSGG 3 cut(s) 1330, 2357, 3416
NcoI CCATGG 1 cut(s) 1698
NdeI CATATG 1 cut(s) 3511
NdeII GATC 9 cut(s) 576, 832, 1182, 1485, 1914, 2599, 3487, 4055, 4153
NlaIV GGNNCC 9 cut(s) 6, 483, 578, 1870, 1890, 2650, 3072, 3090, 3168
NmeAIII GCCGAG 2 cut(s) 369, 2872
NmuCI GTSAC 3 cut(s) 178, 1249, 2621
NspI RCATGY 3 cut(s) 1771, 2692, 3637
NspV TTCGAA 1 cut(s) 663
PagI TCATGA 1 cut(s) 895
PaqCI CACCTGC 1 cut(s) 2431
PceI AGGCCT 1 cut(s) 753
PciI ACATGT 1 cut(s) 1767
PciSI GCTCTTC 5 cut(s) 506, 1521, 1794, 2210, 2983
PctI GAATGC 1 cut(s) 790
PdmI GAANNNNTTC 3 cut(s) 276, 1053, 1693
PflFI GACNNNGTC 1 cut(s) 3786
PfoI TCCNGGA 2 cut(s) 1086, 1383
PleI GAGTC 3 cut(s) 907, 1622, 3580
PpsI GAGTC 3 cut(s) 907, 1622, 3580
PscI ACATGT 1 cut(s) 1767
Psp124BI GAGCTC 1 cut(s) 4125
Psp6I CCWGG 6 cut(s) 1086, 1383, 2014, 2237, 2668, 3007
PspEI GGTNACC 1 cut(s) 2359
PspFI CCCAGC 1 cut(s) 2511
PspGI CCWGG 6 cut(s) 1086, 1383, 2014, 2237, 2668, 3007
PspN4I GGNNCC 9 cut(s) 6, 483, 578, 1870, 1890, 2650, 3072, 3090, 3168
PspPI GGNCC 5 cut(s) 650, 794, 1027, 3412, 4213
PstI CTGCAG 2 cut(s) 1824, 3559
PstNI CAGNNNCTG 1 cut(s) 1828
PsuI RGATCY 3 cut(s) 576, 1182, 4153
PsyI GACNNNGTC 1 cut(s) 3786
PvuII CAGCTG 1 cut(s) 1706
RsaI GTAC 4 cut(s) 1068, 1760, 2095, 2402
RsaNI GTAC 4 cut(s) 1067, 1759, 2094, 2401
RseI CAYNNNNRTG 4 cut(s) 2058, 2628, 2657, 4050
SacI GAGCTC 1 cut(s) 4125
SapI GCTCTTC 5 cut(s) 506, 1521, 1794, 2210, 2983
Sau3AI GATC 9 cut(s) 576, 832, 1182, 1485, 1914, 2599, 3487, 4055, 4153
Sau96I GGNCC 5 cut(s) 650, 794, 1027, 3412, 4213
SchI GAGTC 3 cut(s) 908, 1622, 3581
ScrFI CCNGG 9 cut(s) 1088, 1330, 1385, 2016, 2239, 2357, 2670, 3009, 3416
SduI GDGCHC 3 cut(s) 1615, 3657, 4125
SfaNI GCATC 8 cut(s) 904, 1378, 1740, 2281, 2780, 3069, 3291, 3588
SfcI CTRYAG 3 cut(s) 1820, 2775, 3555
SfuI TTCGAA 1 cut(s) 663
SinI GGWCC 4 cut(s) 650, 1027, 3412, 4213
SmiMI CAYNNNNRTG 4 cut(s) 2058, 2628, 2657, 4050
SmlI CTYRAG 7 cut(s) 308, 564, 770, 1562, 2305, 2340, 3361
SmoI CTYRAG 7 cut(s) 308, 564, 770, 1562, 2305, 2340, 3361
SseBI AGGCCT 1 cut(s) 753
SspI AATATT 5 cut(s) 683, 810, 1945, 2842, 3796
SspMI CTAG 6 cut(s) 755, 855, 1478, 1779, 2481, 3254
SstI GAGCTC 1 cut(s) 4125
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 9 cut(s) 1086, 1328, 1383, 2014, 2237, 2355, 2668, 3007, 3414
StyI CCWWGG 6 cut(s) 653, 948, 1030, 1698, 2480, 3156
TaiI ACGT 5 cut(s) 406, 894, 2478, 2555, 3001
TatI WGTACW 2 cut(s) 1758, 2093
TauI GCSGC 2 cut(s) 1409, 4029
TscAI CASTG 5 cut(s) 366, 1309, 1615, 1978, 3600
TseFI GTSAC 3 cut(s) 178, 1249, 2621
Tsp45I GTSAC 3 cut(s) 178, 1249, 2621
TspGWI ACGGA 2 cut(s) 2833, 3993
TspRI CASTG 5 cut(s) 366, 1309, 1615, 1978, 3600
Tth111I GACNNNGTC 1 cut(s) 3786
VneI GTGCAC 1 cut(s) 1611
VpaK11BI GGWCC 4 cut(s) 650, 1027, 3412, 4213
XagI CCTNNNNNAGG 4 cut(s) 72, 289, 396, 2013
XapI RAATTY 2 cut(s) 1544, 3694
XceI RCATGY 3 cut(s) 1771, 2692, 3637
XcmI CCANNNNNNNNNTGG 1 cut(s) 2371
XmaJI CCTAGG 1 cut(s) 2480
XmiI GTMKAC 1 cut(s) 1626
XmnI GAANNNNTTC 3 cut(s) 276, 1053, 1693
XspI CTAG 6 cut(s) 755, 855, 1478, 1779, 2481, 3254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.