Rroxscaffold_3G00249850

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007888
Physical Location & Seq
Forward (+)
43603790 .. 43609564
5775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00249850.1

Sequence Viewer

Length: 4377 bp
ATGGCTCCAAAGAAGAAGCAGCCGCAGCAGCAGCCCAAGAAGCCCAGCAGTAAACCCCAACCGGCCTCGTCTGGCCAGAAGCCCCAGCCCAACACCGCCAAACCCCAAACGACGTCGTCCGGCCCCAGGCTCCAGATTTCCGCCGAGAACGAGAACCGCCTCCGCCGCCTCCTCCTCAACTCCGGCCGGTCCGCCGCGGTCCCGGTCGACGAGTCGCTCTCTAAGGCTCAGAAGGCCAAGAAGCTCGGGGCCTTGTACGAGCAGCTCTCCTGCGAAGGCTTCACCAATGATCAGATTGAACTCGCTCTCTCCGCCGTCAAGGAAGGGGCTACATATGAGGCTGCTCTGGACTGGTTGTGCTTAAATGTGCCTGGGAATGAGCTTCCACTCAAGTTTTCGAGTGGTGTTTCGATGGCTGCAAATGAAGGTGGTTCTGTTGGTGTCGTACTGACTTCTCGGGACGACTGGACTCCGTCGGTGGATACCTCTGCTAAGATTGATGAGGATACGCCGGGGATTGCTATTAGGACCAAGGGGCAGTGGAATGATAAAACACTTGATTCATGTCAACCGTCTCAGGCAGATTGGATCAAACGATATGTTGAGCAGCAAGAAGAGGATGAATCGAGTACATGGGAAGATGATGTAGATGATGAGGGTATGGGTACTAAGGTCAGTAAACCAAGGTCTTATGATGTTATAGCCAAAGAGTACCACGCTGCGCGGTTGGAAGCTGCAGAAGCCAAGCAACAAAAGGACAAGAAAAACCAGGAACGGGCAGGCAAAATGATACGTGATCTCAAGCAAGAGTTGGCTGCTCTAGGCTTATCGGACGACATCTTGGCATCGGAATATGAGCAGGAACAGTCGATAGAACGAGCTTATTCTGCTTTTGGGGACACAGATGCTAGTTCAATGCCTTACAAGCAGTCTGAAGGACTCCATGCTGATGAATTAAAAGTAGACGGAAATGATATGGAACACTGCAGCTCCGTTGAGCTCCCAATCAACTCTACTCCTTCAGATTTACCTGTTCAGGAAAAAATTGTTGCAGAAGAAGAGACAACTGATATGGAAATTGGTAATTTTTTCTTGGAAGATGCACCATCTAATGAATTCCTAACTCCTACTATTTTGGAACTACAGAAGAAAGAAAAGTTGAGAGAAATGCTCAGTGAGAAAAATTTGGAGAAATTAGATGGTATTTGGAAGAAGGGGGATCCTCAAAAGATTCCCAAGGCTATTTTTCAGCAATTATGTCAAAAATCAGGATGGGAAGCCCCAAAGTTTAATAAAGTGTGGGGAAAGGATAATAGTTTCTCTTATACTATCAGTGTTTTGCGTAAAGCCAGTGGAAGGGGTAAGAGCAGAAAGGCTGGGGGCTTGGTCACTCTTCAGCTTCCTGATCAGGATGTAACTTCTGATTCAGCTGAGGATGCACAAAATAGAGTGGCAGCATATGCTCTTTGTCAACTGTTTCCTGATCTCCCAATCCATCTTGTAATTACTGAGCCCTATGCTTCTCTAGTTGTTCAATGGAAGGAAGGGGAAACAATAACCAATGTAGAAGATAGTGTTAAGGACCGAAGAGCTACTTTTGTGGATTCACTATTGAAAGCTGATGGTTCTACTTCAACTTCTGCTGCTAATGTTGTATATGATTCTGATTCTGATTCTGATTCTCTTCCTAAAGTGGTTCCACAACTCCAGGTTCAAGAACCCAGAAATGCAGAACTAAATCCTCGCAAAGATGCAGAGAGCTCTTATTTGAAACAAGAACTGGAGAATAAACAGAAAAAGCAGAAATTCCAGGAAATGTTGAGAGCTAGAGCTGCACTCCCCATTGCTGGTTTGAAGGGTGATATATTGCAACTCTTGCAGGATAATAATGTTCTAGTAGTTTGTGGGGAAACGGGATCTGGAAAAACAACTCAGGTTCCACAATTTATATTGGATGACATGATCCAGTCAGGACGTGGTGGACACTGTAACATTATATGCACACAACCAAGGAGAATAGCGGCTATTTCTGTGGCTGACAGAGTCGCTGATGAGCGTTGTGAACCTTCACCTGGTTCAAATGGTTCTTTGGTTGGTTATCAAGTACGCCTTGATAATGCAAGTAATGAAAAGACTAAGCTTCTTTTTTGCACAACAGGTATTCTTCTGAGAAAATTCGTGGGAGACAGACACTTGACTGGAATTACTCATGTCATAGTTGATGAAGTACATGAGCGGTCTCTTTTGGGTGATTTCCTGCTCATAGTTTTGAAGAATCTGATTGAGAAGCAATCAGCTCTTAACACACCAAAACTGAAAGTTATTCTAATGTCTGCAACTGTTGATTCCAATTTGTTTTCAAATTACTTTGGTGGTTGTCCTGTGATTACCGCAGAAGGTAGAACACATCCAGTGACGACTTATTTTCTTGAGGATATTTATGAGCGCATTGATTATCGGCTTGCATCAGATTCTCCGGCTTCCATGGTCTATGGAACTTCGACCGAAGGGAAGACTGGTCCTGTAAACAACAGTAGAGGAAAGAAGAATCTTGTTTTATCTGGCTGGGGTGATGATTCTCTACTCTCTGAAGAATATATTAATCCAAATTATGTTCATGATACGTATCAATCTTACAGAGAGCAAACTCGACAAAATTTGAAAAGATTGAATGAAGATGTCATTGATTATGATCTTCTTGAAGACTTGGTGTGCCATGTTGATGAAACTTGCGGCGAAGGTGCTATTCTTGTCTTCTTGCCTGGAGTATCTGAAATTTACACCTTAGTTGATAAACTAGCTGCTTCTTACCGGTTTGGTGGAGCAGCTTCTGATTGGATCCTCCCGCTACATTCATCTGTTGCATCAGTTGATCAAAAGAAGGTGTTTTTGCAGGCTCCTGAGAACATACGAAAGATTATTGTAGCCACTAATATTGCTGAGACCAGCATAACAATAGATGATGTGGTCTATGTTATTGACTGTGGAAAGCATAAGGAGAATCGTTATAATCCCCAGAAGAAATTGTCGAGCATGGTTGAGGATTGGATTTCCAGAGCAAATGCAAGGCAACGTCGAGGACGAGCTGGCCGTGTGAAACCTGGAATCTGCTTTTGCCTGTACACTAGTTATCGATTTGAAAAGCTGATGCGCCCCTTTCAGGTTCCAGAGATGCTTCGAATGCCATTAGTAGAGTTGTGTTTACAAATTAAGTTACTTTCTCTGGGCCACATAAAACCATTTTTGTCCCAGGCTTTGGAACCTCCAAGGGAAGAGGCTATGACTTCAGCAATTAAGATATTGTATGAGGTTGGTGCTCTTGAAACGGATGAAGAGTTAACGCCTCTTGGGCATCATTTGGCGAAACTTCCTGTTGACGTATTGATTGGGAAGATGATGATATATGGTGGAATATTTGGTTGCTTATCACCCATTCTATCAATTTCAGCATTCTTAAGTTACAAATCTCCATTTGTCCATCCAAAAGATGAGAAGGAAAATGCTAAAAGAGCAAAATTGGCACTGTTGACTGACAAGCTAGATGGTCCAAGTGAATCAAATGATGTTGACAAGCAATCTGACCATCTCATAATGATAACTGCATACAAGAAATGGGAAAAGATTTTACGTGACAAAGGGGTTAGAGTTGCGCAGCAGTTTTGCAATTCATATTATTTGAGCAGTTCAGTGATGTACATGATAAAAGACATGAGGATACAGTTTGGAACACTGCTTGCTGATATTGGACTCATTGATCTTCCAAAGAAATATCAGGTTGATGGAAGAAAGAAAGAGAATCTTGATGCTTGGTTTTCGGATGCATCGCAGCCCTTCAATATGTATTCAAACCATTCACCAATTGTAAAAGCTATCATATGTGCAGGTTTATACCCTAACATTGCAGCCACTGAGAAAGGCATTGCTGGAACAGTCCTCAATAACCTTAAACAGGCTCCTGGTCACGCAACTAGCCATTGTCCAACCTGGTATGATGGAAGAAGAAAAGTCAATGTACACCCTTCTTCTATCAACCATAATGTAAATGAATTTCGGTATCCTTTCCTTGTCTTCCTCGAAAAAGTTGAAACCAACAAAGTATTTTTGCGGGACTCTACAATCATTTCTCCCAACTCTATTTTGCTGTTTGGTGGCTCCATTAACATCCAGCATCAGACTGGACTTGTAATTGTTGATGGATGGTTAAAACTGACAGCACCAGCACAGACTGCTGTCCTGTTCAAGGAACTCCGTTTAACCCTTCACTCTGTTCTGAAAGAATTGATAAGAAAGCCTGAGAATTGCACTGTTGGTCATAACGAGGTTTTAAGGTCTATTATCCATTTGTTGTTGGAAGAAGACAAGCCTCCTCCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016282 GO:0032991 GO:0043021 GO:0043024 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0070993 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1458

Amino Acids

162.27

Weight (kDa)

5.88

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_DHX29 PF24899 81 - 133 6e-17 ATP-dependent RNA helicase DHX29, UBA domain
DEAD PF00270 624 - 784 2.4e-10 DEAD/DEAH box helicase
Helicase_C PF00271 909 - 1032 1.4e-09 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1097 - 1125 2.7e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1129 - 1197 3.2e-10 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1288 - 1386 9.3e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1393 - 1450 1e-15 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3009
AatII GACGTC 1 cut(s) 116
Acc16I TGCGCA 1 cut(s) 3651
Acc36I ACCTGC 1 cut(s) 3872
AccB7I CCANNNNNTGG 1 cut(s) 3256
AccBSI CCGCTC 1 cut(s) 2236
AccI GTMKAC 2 cut(s) 207, 963
AccII CGCG 2 cut(s) 197, 724
AclWI GGATC 7 cut(s) 596, 1214, 1227, 1924, 1957, 2833, 2846
AcoI YGGCCR 3 cut(s) 73, 184, 3088
AcsI RAATTY 7 cut(s) 1115, 1183, 1805, 2174, 2656, 2775, 4046
AcuI CTGAAG 5 cut(s) 954, 1005, 1379, 2610, 3270
AcyI GRCGYC 1 cut(s) 113
AfiI CCNNNNNNNGG 9 cut(s) 126, 775, 1356, 1847, 2072, 3160, 3256, 3949, 4240
AflII CTTAAG 1 cut(s) 3454
AgeI ACCGGT 1 cut(s) 2811
AhlI ACTAGT 1 cut(s) 3125
AjiI CACGTC 1 cut(s) 1976
Alw21I GWGCWC 3 cut(s) 1002, 1763, 3319
Alw26I GTCTC 5 cut(s) 579, 1055, 2178, 2244, 2936
AlwI GGATC 7 cut(s) 596, 1214, 1227, 1924, 1957, 2833, 2846
AlwNI CAGNNNCTG 2 cut(s) 2831, 3908
Ama87I CYCGRG 2 cut(s) 245, 456
AoxI GGCC 8 cut(s) 63, 73, 121, 184, 234, 249, 3088, 3226
ApoI RAATTY 7 cut(s) 1115, 1183, 1805, 2174, 2656, 2775, 4046
AseI ATTAAT 1 cut(s) 2601
AsiGI ACCGGT 1 cut(s) 2811
AspLEI GCGC 4 cut(s) 724, 2448, 3153, 3652
AspS9I GGNCC 9 cut(s) 122, 189, 199, 249, 528, 1582, 2519, 3226, 3545
AsuC2I CCSGG 2 cut(s) 203, 513
AsuHPI GGTGA 7 cut(s) 274, 1871, 2061, 2261, 2582, 3420, 3846
AsuII TTCGAA 1 cut(s) 3178
AvaI CYCGRG 2 cut(s) 245, 456
AvaII GGWCC 6 cut(s) 189, 199, 528, 1582, 2519, 3545
BaeI ACNNNNGTAYC 2 cut(s) 657, 690
BalI TGGCCA 1 cut(s) 75
BamHI GGATCC 2 cut(s) 1219, 2838
BanII GRGCYC 3 cut(s) 1002, 1515, 1763
BbsI GAAGAC 5 cut(s) 2519, 2709, 2746, 4060, 4362
Bbv12I GWGCWC 3 cut(s) 1002, 1763, 3319
BbvCI CCTCAGC 1 cut(s) 1431
BceAI ACGGC 2 cut(s) 299, 3075
BciVI GTATCC 4 cut(s) 475, 499, 3708, 4065
BclI TGATCA 3 cut(s) 289, 1405, 2872
BcnI CCSGG 2 cut(s) 203, 513
BcoDI GTCTC 5 cut(s) 579, 1055, 2178, 2244, 2936
BcuI ACTAGT 1 cut(s) 3125
BfaI CTAG 9 cut(s) 821, 909, 1526, 1827, 1895, 2798, 3126, 3539, 3969
BfmI CTRYAG 3 cut(s) 735, 985, 1142
BfrI CTTAAG 1 cut(s) 3454
BfuAI ACCTGC 1 cut(s) 3872
BfuI GTATCC 4 cut(s) 475, 499, 3708, 4065
BglI GCCNNNNNGGC 1 cut(s) 3349
Bme18I GGWCC 6 cut(s) 189, 199, 528, 1582, 2519, 3545
BmeT110I CYCGRG 2 cut(s) 245, 456
BmgBI CACGTC 1 cut(s) 1976
BmgT120I GGNCC 9 cut(s) 122, 189, 199, 249, 528, 1582, 2519, 3226, 3545
BoxI GACNNNNGTC 1 cut(s) 4229
BpiI GAAGAC 5 cut(s) 2519, 2709, 2746, 4060, 4362
BplI GAGNNNNNCTC 8 cut(s) 203, 235, 251, 283, 1155, 1187, 1821, 1853
BpmI CTGGAG 4 cut(s) 116, 1691, 1802, 2784
Bpu10I CCTNAGC 1 cut(s) 1431
Bpu14I TTCGAA 1 cut(s) 3178
BpuEI CTTGAG 3 cut(s) 374, 785, 2450
BpuMI CCSGG 2 cut(s) 203, 513
Bsa29I ATCGAT 1 cut(s) 3133
BsaAI YACGTR 3 cut(s) 794, 2625, 3629
BsaBI GATNNNNATC 2 cut(s) 2625, 2691
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 2 cut(s) 2244, 2936
BsaWI WCCGGW 1 cut(s) 2811
BsaXI ACNNNNNCTCC 2 cut(s) 974, 1004
Bsc4I CCNNNNNNNGG 9 cut(s) 126, 775, 1356, 1847, 2072, 3160, 3256, 3949, 4240
Bse118I RCCGGY 3 cut(s) 61, 186, 2811
Bse1I ACTGG 9 cut(s) 356, 470, 1350, 1785, 1966, 2203, 2411, 2521, 4180
Bse3DI GCAATG 3 cut(s) 1842, 3897, 3918
Bse8I GATNNNNATC 2 cut(s) 2625, 2691
BseCI ATCGAT 1 cut(s) 3133
BseJI GATNNNNATC 2 cut(s) 2625, 2691
BseLI CCNNNNNNNGG 9 cut(s) 126, 775, 1356, 1847, 2072, 3160, 3256, 3949, 4240
BseMI GCAATG 3 cut(s) 1842, 3897, 3918
BseNI ACTGG 9 cut(s) 356, 470, 1350, 1785, 1966, 2203, 2411, 2521, 4180
BseRI GAGGAG 4 cut(s) 161, 164, 4356, 4359
BseX3I CGGCCG 1 cut(s) 184
BseYI CCCAGC 4 cut(s) 44, 84, 1376, 2565
BsgI GTGCAG 2 cut(s) 1818, 3900
Bsh1236I CGCG 2 cut(s) 197, 724
Bsh1285I CGRYCG 3 cut(s) 187, 207, 2505
BshFI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3090, 3228
BshTI ACCGGT 1 cut(s) 2811
BshVI ATCGAT 1 cut(s) 3133
BsiEI CGRYCG 3 cut(s) 187, 207, 2505
BsiHKAI GWGCWC 3 cut(s) 1002, 1763, 3319
BsiHKCI CYCGRG 2 cut(s) 245, 456
BsiSI CCGG 8 cut(s) 62, 120, 183, 187, 203, 512, 2477, 2812
BslFI GGGAC 5 cut(s) 185, 473, 911, 3232, 4121
BslI CCNNNNNNNGG 9 cut(s) 126, 775, 1356, 1847, 2072, 3160, 3256, 3949, 4240
BsmAI GTCTC 5 cut(s) 579, 1055, 2178, 2244, 2936
BsmBI CGTCTC 1 cut(s) 579
BsmFI GGGAC 5 cut(s) 185, 473, 911, 3232, 4121
BsmI GAATGC 2 cut(s) 3186, 3449
BsnI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3090, 3228
Bso31I GGTCTC 2 cut(s) 2244, 2936
BsoBI CYCGRG 2 cut(s) 245, 456
Bsp119I TTCGAA 1 cut(s) 3178
Bsp1286I GDGCHC 4 cut(s) 1002, 1515, 1763, 3319
Bsp1407I TGTACA 3 cut(s) 3120, 3693, 4012
Bsp19I CCATGG 1 cut(s) 2484
BspANI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3090, 3228
BspDI ATCGAT 1 cut(s) 3133
BspFNI CGCG 2 cut(s) 197, 724
BspHI TCATGA 1 cut(s) 2617
BspMAI CTGCAG 2 cut(s) 739, 989
BspMI ACCTGC 1 cut(s) 3872
BspPI GGATC 7 cut(s) 596, 1214, 1227, 1924, 1957, 2833, 2846
BspQI GCTCTTC 1 cut(s) 1582
BspT104I TTCGAA 1 cut(s) 3178
BspTI CTTAAG 1 cut(s) 3454
BspTNI GGTCTC 2 cut(s) 2244, 2936
BsrBI CCGCTC 1 cut(s) 2236
BsrDI GCAATG 3 cut(s) 1842, 3897, 3918
BsrFI RCCGGY 3 cut(s) 61, 186, 2811
BsrGI TGTACA 3 cut(s) 3120, 3693, 4012
BsrI ACTGG 9 cut(s) 356, 470, 1350, 1785, 1966, 2203, 2411, 2521, 4180
BssAI RCCGGY 3 cut(s) 61, 186, 2811
BssNI GRCGYC 1 cut(s) 113
BssT1I CCWWGG 6 cut(s) 531, 683, 1236, 2009, 2484, 3266
Bst6I CTCTTC 7 cut(s) 609, 1053, 1398, 1582, 1689, 3267, 3327
BstACI GRCGYC 1 cut(s) 113
BstAFI CTTAAG 1 cut(s) 3454
BstAPI GCANNNNNTGC 3 cut(s) 1460, 1876, 4226
BstAUI TGTACA 3 cut(s) 3120, 3693, 4012
BstBAI YACGTR 3 cut(s) 794, 2625, 3629
BstBI TTCGAA 1 cut(s) 3178
BstC8I GCNNGC 5 cut(s) 781, 2463, 2895, 3088, 3735
BstDSI CCRYGG 2 cut(s) 195, 2484
BstENI CCTNNNNNAGG 2 cut(s) 3947, 4238
BstFNI CGCG 2 cut(s) 197, 724
BstHHI GCGC 4 cut(s) 724, 2448, 3153, 3652
BstMAI GTCTC 5 cut(s) 579, 1055, 2178, 2244, 2936
BstMCI CGRYCG 3 cut(s) 187, 207, 2505
BstPAI GACNNNNGTC 1 cut(s) 4229
BstSFI CTRYAG 3 cut(s) 735, 985, 1142
BstSNI TACGTA 1 cut(s) 2625
BstUI CGCG 2 cut(s) 197, 724
BstV2I GAAGAC 5 cut(s) 2519, 2709, 2746, 4060, 4362
BstX2I RGATCY 3 cut(s) 1219, 1916, 2838
BstYI RGATCY 3 cut(s) 1219, 1916, 2838
BstZI CGGCCG 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 3133
BsuI GTATCC 4 cut(s) 475, 499, 3708, 4065
BsuRI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3090, 3228
BsuTUI ATCGAT 1 cut(s) 3133
BtgI CCRYGG 2 cut(s) 195, 2484
BtgZI GCGATG 1 cut(s) 3807
BtrI CACGTC 1 cut(s) 1976
BtsI GCAGTG 3 cut(s) 545, 982, 3728
BveI ACCTGC 1 cut(s) 3872
Cac8I GCNNGC 5 cut(s) 781, 2463, 2895, 3088, 3735
CaiI CAGNNNCTG 2 cut(s) 2831, 3908
CciI TCATGA 1 cut(s) 2617
CfoI GCGC 4 cut(s) 724, 2448, 3153, 3652
Cfr10I RCCGGY 3 cut(s) 61, 186, 2811
Cfr13I GGNCC 9 cut(s) 122, 189, 199, 249, 528, 1582, 2519, 3226, 3545
Cfr42I CCGCGG 1 cut(s) 198
ClaI ATCGAT 1 cut(s) 3133
CpoI CGGWCCG 1 cut(s) 189
CsiI ACCWGGT 2 cut(s) 2071, 3983
CspAI ACCGGT 1 cut(s) 2811
CspCI CAANNNNNGTGG 2 cut(s) 2917, 2952
CspI CGGWCCG 1 cut(s) 189
EaeI YGGCCR 3 cut(s) 73, 184, 3088
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 7 cut(s) 609, 1053, 1398, 1582, 1689, 3267, 3327
EarI CTCTTC 7 cut(s) 609, 1053, 1398, 1582, 1689, 3267, 3327
EciI GGCGGA 4 cut(s) 130, 152, 181, 301
Ecl136II GAGCTC 2 cut(s) 1000, 1761
EclXI CGGCCG 1 cut(s) 184
Eco105I TACGTA 1 cut(s) 2625
Eco130I CCWWGG 6 cut(s) 531, 683, 1236, 2009, 2484, 3266
Eco24I GRGCYC 3 cut(s) 1002, 1515, 1763
Eco31I GGTCTC 2 cut(s) 2244, 2936
Eco47I GGWCC 6 cut(s) 189, 199, 528, 1582, 2519, 3545
Eco52I CGGCCG 1 cut(s) 184
Eco53kI GAGCTC 2 cut(s) 1000, 1761
Eco57I CTGAAG 5 cut(s) 954, 1005, 1379, 2610, 3270
Eco88I CYCGRG 2 cut(s) 245, 456
EcoICRI GAGCTC 2 cut(s) 1000, 1761
EcoNI CCTNNNNNAGG 2 cut(s) 3947, 4238
EcoO109I RGGNCCY 1 cut(s) 249
EcoRI GAATTC 1 cut(s) 1115
EcoT14I CCWWGG 6 cut(s) 531, 683, 1236, 2009, 2484, 3266
EcoT22I ATGCAT 1 cut(s) 3823
EcoT38I GRGCYC 3 cut(s) 1002, 1515, 1763
ErhI CCWWGG 6 cut(s) 531, 683, 1236, 2009, 2484, 3266
Esp3I CGTCTC 1 cut(s) 579
FalI AAGNNNNNCTT 6 cut(s) 1579, 1611, 2094, 2126, 3783, 3815
FaqI GGGAC 5 cut(s) 185, 473, 911, 3232, 4121
FauI CCCGC 2 cut(s) 2853, 4098
FauNDI CATATG 3 cut(s) 334, 1459, 3875
FbaI TGATCA 3 cut(s) 289, 1405, 2872
FblI GTMKAC 2 cut(s) 207, 963
FriOI GRGCYC 3 cut(s) 1002, 1515, 1763
FspBI CTAG 9 cut(s) 821, 909, 1526, 1827, 1895, 2798, 3126, 3539, 3969
FspI TGCGCA 1 cut(s) 3651
GlaI GCGC 4 cut(s) 723, 2447, 3152, 3651
GsaI CCCAGC 4 cut(s) 48, 88, 1380, 2569
GsuI CTGGAG 4 cut(s) 116, 1691, 1802, 2784
HaeIII GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3090, 3228
HapII CCGG 8 cut(s) 62, 120, 183, 187, 203, 512, 2477, 2812
HhaI GCGC 4 cut(s) 724, 2448, 3153, 3652
Hin1I GRCGYC 1 cut(s) 113
Hin6I GCGC 4 cut(s) 722, 2446, 3151, 3650
HinP1I GCGC 4 cut(s) 722, 2446, 3151, 3650
HincII GTYRAC 7 cut(s) 208, 569, 1472, 3339, 3376, 3528, 3568
HindII GTYRAC 7 cut(s) 208, 569, 1472, 3339, 3376, 3528, 3568
HindIII AAGCTT 1 cut(s) 2138
HpaI GTTAAC 1 cut(s) 3339
HpaII CCGG 8 cut(s) 62, 120, 183, 187, 203, 512, 2477, 2812
HphI GGTGA 7 cut(s) 274, 1871, 2061, 2261, 2582, 3420, 3846
Hpy99I CGWCG 5 cut(s) 115, 118, 212, 478, 3078
HpyCH4IV ACGT 7 cut(s) 113, 793, 1975, 2624, 3073, 3378, 3628
HpySE526I ACGT 7 cut(s) 113, 793, 1975, 2624, 3073, 3378, 3628
Hsp92I GRCGYC 1 cut(s) 113
HspAI GCGC 4 cut(s) 722, 2446, 3151, 3650
Ksp22I TGATCA 3 cut(s) 289, 1405, 2872
KspAI GTTAAC 1 cut(s) 3339
KspI CCGCGG 1 cut(s) 198
LguI GCTCTTC 1 cut(s) 1582
LmnI GCTCC 8 cut(s) 10, 135, 995, 1005, 2822, 2902, 3958, 4157
MabI ACCWGGT 2 cut(s) 2071, 3983
MaeI CTAG 9 cut(s) 821, 909, 1526, 1827, 1895, 2798, 3126, 3539, 3969
MaeII ACGT 7 cut(s) 113, 793, 1975, 2624, 3073, 3378, 3628
MaeIII GTNAC 8 cut(s) 1387, 1414, 1988, 2413, 3213, 3458, 3629, 3959
MbiI CCGCTC 1 cut(s) 2236
MfeI CAATTG 1 cut(s) 3858
MflI RGATCY 3 cut(s) 1219, 1916, 2838
MhlI GDGCHC 4 cut(s) 1002, 1515, 1763, 3319
MlsI TGGCCA 1 cut(s) 75
MluNI TGGCCA 1 cut(s) 75
MlyI GAGTC 6 cut(s) 221, 463, 933, 2052, 3741, 4103
MmeI TCCRAC 3 cut(s) 708, 4004, 4329
Mox20I TGGCCA 1 cut(s) 75
Mph1103I ATGCAT 1 cut(s) 3823
MscI TGGCCA 1 cut(s) 75
MslI CAYNNNNRTG 2 cut(s) 948, 2721
Msp20I TGGCCA 1 cut(s) 75
MspA1I CMGCKG 2 cut(s) 197, 1430
MspCI CTTAAG 1 cut(s) 3454
MspI CCGG 8 cut(s) 62, 120, 183, 187, 203, 512, 2477, 2812
MunI CAATTG 1 cut(s) 3858
Mva1269I GAATGC 2 cut(s) 3186, 3449
MvnI CGCG 2 cut(s) 197, 724
NciI CCSGG 2 cut(s) 203, 513
NcoI CCATGG 1 cut(s) 2484
NdeI CATATG 3 cut(s) 334, 1459, 3875
NmeAIII GCCGAG 1 cut(s) 169
NmuCI GTSAC 4 cut(s) 1387, 2413, 3629, 3959
NsbI TGCGCA 1 cut(s) 3651
NsiI ATGCAT 1 cut(s) 3823
NspV TTCGAA 1 cut(s) 3178
PagI TCATGA 1 cut(s) 2617
PciSI GCTCTTC 1 cut(s) 1582
PcsI WCGNNNNNNNCGW 2 cut(s) 3079, 3088
PctI GAATGC 2 cut(s) 3186, 3449
PflFI GACNNNGTC 3 cut(s) 115, 472, 2042
PflMI CCANNNNNTGG 1 cut(s) 3256
PfoI TCCNGGA 1 cut(s) 1809
PinAI ACCGGT 1 cut(s) 2811
PleI GAGTC 6 cut(s) 220, 463, 933, 2051, 3741, 4103
PpsI GAGTC 6 cut(s) 220, 463, 933, 2051, 3741, 4103
Ppu21I YACGTR 3 cut(s) 794, 2625, 3629
PshAI GACNNNNGTC 1 cut(s) 4229
PshBI ATTAAT 1 cut(s) 2601
PsiI TTATAA 1 cut(s) 3009
Psp124BI GAGCTC 2 cut(s) 1002, 1763
PspFI CCCAGC 4 cut(s) 44, 84, 1376, 2565
PspPI GGNCC 9 cut(s) 122, 189, 199, 249, 528, 1582, 2519, 3226, 3545
PstI CTGCAG 2 cut(s) 739, 989
PstNI CAGNNNCTG 2 cut(s) 2831, 3908
PsuI RGATCY 3 cut(s) 1219, 1916, 2838
PsyI GACNNNGTC 3 cut(s) 115, 472, 2042
PvuII CAGCTG 1 cut(s) 1430
RseI CAYNNNNRTG 2 cut(s) 948, 2721
Rsr2I CGGWCCG 1 cut(s) 189
RsrII CGGWCCG 1 cut(s) 189
SacI GAGCTC 2 cut(s) 1002, 1763
SacII CCGCGG 1 cut(s) 198
SalI GTCGAC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 1582
Sau96I GGNCC 9 cut(s) 122, 189, 199, 249, 528, 1582, 2519, 3226, 3545
SchI GAGTC 6 cut(s) 221, 463, 933, 2052, 3741, 4103
SduI GDGCHC 4 cut(s) 1002, 1515, 1763, 3319
SexAI ACCWGGT 2 cut(s) 2071, 3983
SfcI CTRYAG 3 cut(s) 735, 985, 1142
Sfr303I CCGCGG 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 3178
SgrBI CCGCGG 1 cut(s) 198
SinI GGWCC 6 cut(s) 189, 199, 528, 1582, 2519, 3545
SmiMI CAYNNNNRTG 2 cut(s) 948, 2721
SmlI CTYRAG 4 cut(s) 389, 800, 2429, 3454
SmoI CTYRAG 4 cut(s) 389, 800, 2429, 3454
SnaBI TACGTA 1 cut(s) 2625
SpeI ACTAGT 1 cut(s) 3125
SspI AATATT 2 cut(s) 2935, 3414
SspMI CTAG 9 cut(s) 821, 909, 1526, 1827, 1895, 2798, 3126, 3539, 3969
SstI GAGCTC 2 cut(s) 1002, 1763
StyI CCWWGG 6 cut(s) 531, 683, 1236, 2009, 2484, 3266
TaiI ACGT 7 cut(s) 116, 796, 1978, 2627, 3076, 3381, 3631
TaqII GACCGA 2 cut(s) 1599, 2519
TatI WGTACW 5 cut(s) 629, 2227, 3120, 3693, 4012
TauI GCSGC 5 cut(s) 25, 168, 197, 2024, 2736
TseFI GTSAC 4 cut(s) 1387, 2413, 3629, 3959
Tsp45I GTSAC 4 cut(s) 1387, 2413, 3629, 3959
TspGWI ACGGA 5 cut(s) 462, 981, 982, 3341, 4238
Tth111I GACNNNGTC 3 cut(s) 115, 472, 2042
Van91I CCANNNNNTGG 1 cut(s) 3256
Vha464I CTTAAG 1 cut(s) 3454
VpaK11BI GGWCC 6 cut(s) 189, 199, 528, 1582, 2519, 3545
VspI ATTAAT 1 cut(s) 2601
XagI CCTNNNNNAGG 2 cut(s) 3947, 4238
XapI RAATTY 7 cut(s) 1115, 1183, 1805, 2174, 2656, 2775, 4046
XcmI CCANNNNNNNNNTGG 2 cut(s) 1973, 4172
XmiI GTMKAC 2 cut(s) 207, 963
XspI CTAG 9 cut(s) 821, 909, 1526, 1827, 1895, 2798, 3126, 3539, 3969
ZraI GACGTC 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 3823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.