AT1G58060

Helicase associated domain (HA2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007888
Physical Location & Seq
Reverse (-)
21489284 .. 21501890
12607 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G58060.1

Sequence Viewer

Length: 4380 bp
ATGGCTCCGAAGAAGAAACCTCAGAAGCAAAGCAACAAAGCTGCTTCGTCTTCTTCATCGTCGAAATCGAATTATCAGAAACCTTCTTCAGGTCCTAAGCTTCAGATATCAGCGGAGAACGAGGATCGTTTGCGGCGGCTCTTACTCAACTCTGGCCGCTCCGGTCCTTCGATTCCAGCTCCAATCAGTAATTCTCTCTCCAAAGCTCAAAAGACAAAGAAACTCAATAATGTCTATGAGAAGCTCTCTTGCGAAGGTTTCGTTGATGATCAGATTGAACTCGCTCTCTCCTCTCTTAGAGATGGAGCTACGTTTGAGGCTGCACTTGATTGGCTTTGTTTGAATTTGCCGAGTCATGAGTTGCCTGTGAAGTTTTCTACTGGAGCTTCTCGTTTTCCTTCCACAGGAGGTTCTGTGGGTGTGATATCGACCTCACGGGACGATTGGAATGATTCTACTGACTCTTCAGTCAGGGTAGAGGAAGAGGAACCTGCAGTTTTTGTTCGTGTGAAGGGAAAGCAAGATGAAGAAGATACTCTTAGTTCTGACAAGTCATCTCAAGCTGATTGGATTCGGCAGTATATGATGAGACAGGAAGAGGAAGAATTGGAATGTTGGGAAGATGAAGTAGATGGTATTGACCCCAGAAATAAGGTCTCTGGTCCAAGGCCATTCGATGTGATTGCCAAAGAATACTATTCGGCAAGATCAGATGCTATAAAAGCAAAGGAGAAGCGTGATAAAAGAGGACAGGAGCAAGCAGGCCTAGCCATTCGCAAGCTCAAGCAAGAGATATCTGATCTTGGGTTATCAGAAGCAATGTTGGAATCTGAATTTCAGCGTGAACATGCTTTTGAAAGTGCTACAGAACAAGAATCAACCTGTCCCATTTCCGATAACCTTCATGAGTCAGTTGATGCTGACGATGTCTCTGTTCAGATGTTAGATAATTTGACCTTGAATACCAATCCTGCTGAGAGCTATGAGTCTGAGGAAATTCAAACTAAAGCTCTTCCCTCAAGTTCCTCGGGACAAGATTTTGTTGCATCAGATGAAGATTCAGAGGACGTGGAACTTGGCGATACATTCTTTGAAGAAATTCCGCCTTCTGAAATTTCACCCCATGAACTCCTGGAATTGCAGAAGGAGGAAAAGATGAGAGAACTACGTAGTGAGAAAAATCTGGGGAAACTAGATGGTATCTGGAAGAAGGGTGAGGCGCAAAAGATCCCAAAAGCATTTCTCCATCAATTGTGTCAAAGATCAGGGTGGGAAGCACCAAAGTTCAATAAAGAAACCGGTGAAGGAAGAAATTTCTCATATACTGTAAGTATTCTGCGCAAAGCCAGTGGGAGGGGTAAAAACAGACAAGCCGGTGGTCTGGTAACTCTCCAACTTCCTCCTAAAGATGAGAATTTTGAATCCATCGAGGATGCACAAAACAAAGTTGCAGCATTTGCTCTTCATAAACTTTTTTCTGATTTACCTGTCCACTTTGCAATTACGGAGCCTTATGCTTCTCTAGTTTTGATCTGGAAACAAGAGGAATTGTTGTGTACTACCATACAAAGCACAGAAGAAGACCGAAGAGCTAATTTTGTTGATAAGTTGCTTGAGGAGGACAGTTTTAGTTTGACCACTTCGTCGAGTAGCTTTGAGAATTCGCTTCCGTTGGTAGACTCCTATGTCAAAGACAAAGATGACTTGGGTGTTGTCAAATCTAACAACAGAGCTAAAAGAGACTCTTATATAGAGGCTGAGTGTTTATCTCTCCAACGAAAACAAGAAAACAAAAAGAGAACGCAAAAGTACAAGGACATGTTGAAAACTAGAACTGCTCTTCCTATATCTGAAGTTAAGAATGGCATACTCCAGCATCTGAAAGAAAAAGATGTCTTGGTGGTATGCGGAGAAACAGGCTCTGGAAAGACTACACAGGTTCCTCAATTTATATTGGATGATATGATTGATTCGGGGCATGGTGGATACTGTAATATCATATGCACACAACCTCGTCGGATAGCGGCTATCTCTGTTGCTCAAAGAGTTGCTGATGAGCGTTGTGAATCCTCTCCAGGCTTAGATGACTCATTGGTTGGGTATCAAGTTCGTCTTGAAAGTGCAAGGAGTGACAAGACTAGGCTGCTGTTTTGTACTACTGGCATTCTACTGAGGAAACTCGCTGGGGATAGAACCTTGAACGATGTTACACATATTATAGTTGATGAAGTGCATGAGCGGTCTCTTTTGGGTGATTTTCTTCTAATTATTTTGAAGAGCCTGATTGAGAAGCAATCATGCGATAATACATCACGTAAACTGAAAGTTATCCTTATGTCTGCTACTGTTGACGCTGATCTATTCTCAAGATATTTTGGTCATTGTCCTGTGATCACTGCTCAAGGACGAACACATCCAGTCACTACCCACTTCCTGGAGGAGATTTATGAGAGTATTAACTACCTTCTTGCTCCAGATTCTCCTGCTGCGCTAAGATCTGATACATCTATTAAAGACAAGCTTGGTTCTGTAAATGACCGTAGGGGGAAAAAGAATCTTGTATTGGCTGGCTGGGGCGACGATTATTTGCTTTCAGAGGACTGTCTTAATCCTTTTTATGTTTCCAGTAATTACAACTCATACAGTGACCAAACACAACAAAATCTGAAAAGGTTAAACGAGGATAGAATTGACTACGAGCTTCTTGAAGAGTTGATATGCCACATTGATGACACTTGTGAGGAAGGAGCCATTCTTATATTTTTGCCTGGAGTGGCAGAAATTTACATGTTACTCGATATGTTAGCTGCTTCCTATCGTTTTCGTGGACCTGCTGCAGATTGGCTTCTTCCTCTACATTCTTCTATTGCATCTTCAGAACAGAGAAAGGTGTTTCTGCGTCCCCCTAAAGGCTTACGGAAGGTTATCGCAGCTACAAATATTGCAGAGACCAGTATAACAATTGATGATGTGGTGTATGTGATTGACAGTGGCAAACACAAGGAAAATCGCTACAATCCACAGAAGAAACTGTCGAGCATGGTGGAAGATTGGATATCTCAAGCAAATGCAAGACAAAGAACAGGAAGAGCTGGACGCGTCAAACCTGGGATTTGCTTTTCATTGTACACACGCTATAGGTTCGAGAAGCTAATGCGTCCCTATCAGGTTCCTGAAATGCTGCGGATGCCATTAGTAGAGTTATGTCTACAAATCAAATTGCTTGGCTTGGGTCACATTAAACCTTTTCTGTCCAGGGCTTTGGAACCTCCGAGTGAAGGTGCTATGACCTCTGCGATTTCATTGTTACATGAGGTTGGTGCTGTTGAAGGGGATGAAGAGTTGACACCACTTGGGCATCATTTAGCCAAACTTCCTGTTGATGTGTTAATTGGAAAGATGCTGTTATACGGTGGAATATTTGGTTGCTTATCACCTATTCTCTCGATCGCAGCTTTCTTGAGCTACAAATCGCCGTTTATATATCCCAAAGATGAGAAGCAAAATGTCGACCGGGTGAAACTTGCTCTTTTGTCTGACAATGGGGTGAGCTCAAGTGATTTAAACAACAATGACAGACAATCTGATCACCTCCTTATGATGGTTGCATACGATAAATGGGTGAAGATACTGCAAGAGCGAGGAATGAAAGCTGCACAGAGGTTCTGTGAATCAAAGTTCTTGAGCAGCTCAGTGATGCGGATGATCAGAGACATGAGAGTACAATTTGGCACATTACTTGCCGACATCGGGCTCATCAATCTTCCTAAAACAGGAGAGTTTTCAGGAAGGAAAAAGGAAAATCTTGATGTCTGGTTTTCTGACCCAACTCAACCTTTCAATATGTATTCACAGCAACCTGAAGTTGTTAAGGCTATACTATGCGCAGGACTATATCCTAATATAGCAGCCAATGATAAAGGAATTACAGAGACAACGTTTAACAGTTTGACAAAGCAAGGAAACCAAACAAAGAGCTACTCGGCATGGTACGATGGCAGAAGGGAAGTTCATATCCATCCATCTTCCATAAACAGCAACTTCAAAGCATTTCAAAACCCTTTCCTTGTATTCCTTGAGAAGGTGGAAACGAACAAAGTATATTTGCGAGATACAACCATTGTTTCTCCTTTCTCTATTCTACTTTTTGGCGGCTCAATCAATGTTCATCATCAGAGTGGATCAGTCACTATTGATGGGTGGTTAAAGGTAGCAGCACCAGCTCAAACCGCGGTTTTGTTCAAAGAACTCCGGTTAACCCTCCATTCCATTCTCAAAGATCTTATCCGAAAACCTGAGAAATCAGGTATTGTCCACAACGAAGTCGTCAAATCGATGGTTCATCTTCTTATAGAAGAAGGAAAACCGCAACACAAATGA

Protein Analysis

1459

Amino Acids

163.6

Weight (kDa)

5.85

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_DHX29 PF24899 74 - 126 4.8e-18 ATP-dependent RNA helicase DHX29, UBA domain
DEAD PF00270 624 - 784 3.7e-11 DEAD/DEAH box helicase
Helicase_C PF00271 903 - 1034 1.2e-09 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1099 - 1127 1.4e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1131 - 1199 3.2e-11 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1290 - 1387 6.8e-23 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1396 - 1450 2.4e-13 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 467, 1642, 1685
Acc16I TGCGCA 2 cut(s) 1340, 3886
Acc36I ACCTGC 2 cut(s) 499, 2839
AccB7I CCANNNNNTGG 1 cut(s) 2434
AccBSI CCGCTC 2 cut(s) 159, 2239
AccI GTMKAC 3 cut(s) 1677, 3208, 3510
AccII CGCG 2 cut(s) 3099, 4232
AclI AACGTT 1 cut(s) 3938
AclWI GGATC 3 cut(s) 132, 1222, 4189
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 9 cut(s) 343, 833, 996, 1098, 1113, 1312, 1414, 1660, 2781
AcuI CTGAAG 6 cut(s) 72, 86, 450, 1872, 2859, 3882
AfaI GTAC 6 cut(s) 1558, 1811, 2155, 3128, 3723, 3992
AflIII ACRYGT 3 cut(s) 1818, 2787, 3097
AgeI ACCGGT 1 cut(s) 1298
AjiI CACGTC 1 cut(s) 1069
AjnI CCWGG 6 cut(s) 1131, 2074, 2433, 2767, 3106, 3254
AjuI GAANNNNNNNTTGG 4 cut(s) 175, 207, 2546, 2578
AloI GAACNNNNNNTCC 2 cut(s) 1825, 1857
Alw21I GWGCWC 1 cut(s) 3554
Alw26I GTCTC 8 cut(s) 583, 661, 934, 1734, 2247, 2942, 3705, 3926
AlwI GGATC 3 cut(s) 132, 1222, 4189
AlwNI CAGNNNCTG 2 cut(s) 1879, 1922
Ama87I CYCGRG 1 cut(s) 1027
AoxI GGCC 3 cut(s) 154, 668, 763
ApoI RAATTY 9 cut(s) 343, 833, 996, 1098, 1113, 1312, 1414, 1660, 2781
AsiGI ACCGGT 1 cut(s) 1298
Asp700I GAANNNNTTC 1 cut(s) 1098
AspLEI GCGC 4 cut(s) 1222, 1341, 2491, 3887
AspS9I GGNCC 4 cut(s) 92, 164, 662, 2828
AsuC2I CCSGG 1 cut(s) 3515
AsuHPI GGTGA 9 cut(s) 1110, 1226, 1313, 2264, 3426, 3529, 3559, 3581, 3634
AvaI CYCGRG 1 cut(s) 1027
AvaII GGWCC 4 cut(s) 92, 164, 662, 2828
BanII GRGCYC 2 cut(s) 3554, 3756
BbsI GAAGAC 2 cut(s) 42, 1587
Bbv12I GWGCWC 1 cut(s) 3554
BceAI ACGGC 1 cut(s) 3460
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BciT130I CCWGG 6 cut(s) 1133, 2076, 2435, 2769, 3108, 3256
BciVI GTATCC 1 cut(s) 1979
BclI TGATCA 4 cut(s) 268, 2391, 3586, 3705
BcnI CCSGG 1 cut(s) 3515
BcoDI GTCTC 8 cut(s) 583, 661, 934, 1734, 2247, 2942, 3705, 3926
BfaI CTAG 5 cut(s) 767, 1193, 1523, 1830, 2139
BfmI CTRYAG 4 cut(s) 492, 864, 2835, 3136
BfuAI ACCTGC 2 cut(s) 499, 2839
BfuI GTATCC 1 cut(s) 1979
BglII AGATCT 2 cut(s) 2495, 4279
Bme1390I CCNGG 7 cut(s) 1133, 2076, 2435, 2769, 3108, 3256, 3515
Bme18I GGWCC 4 cut(s) 92, 164, 662, 2828
BmeT110I CYCGRG 1 cut(s) 1027
BmgBI CACGTC 1 cut(s) 1069
BmgT120I GGNCC 4 cut(s) 92, 164, 662, 2828
BmiI GGNNCC 7 cut(s) 6, 489, 1509, 1941, 2749, 3171, 3267
BmrFI CCNGG 7 cut(s) 1133, 2076, 2435, 2769, 3108, 3256, 3515
BpiI GAAGAC 2 cut(s) 42, 1587
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpmI CTGGAG 6 cut(s) 402, 1856, 2058, 2456, 2457, 2790
Bpu10I CCTNAGC 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 3515
Bsa29I ATCGAT 1 cut(s) 4334
BsaAI YACGTR 2 cut(s) 1169, 2315
BsaI GGTCTC 3 cut(s) 661, 2247, 2942
BsaJI CCNNGG 5 cut(s) 665, 1026, 3107, 3255, 4230
BsaWI WCCGGW 3 cut(s) 161, 1298, 4251
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bse118I RCCGGY 2 cut(s) 1298, 1373
Bse1I ACTGG 6 cut(s) 385, 1347, 2164, 2417, 2625, 2952
Bse3DI GCAATG 1 cut(s) 825
BseBI CCWGG 6 cut(s) 1133, 2076, 2435, 2769, 3108, 3256
BseCI ATCGAT 1 cut(s) 4334
BseDI CCNNGG 5 cut(s) 665, 1026, 3107, 3255, 4230
BseGI GGATG 7 cut(s) 1438, 1963, 2413, 3192, 3340, 3708, 4018
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 7 cut(s) 35, 966, 981, 1749, 2162, 3705, 4287
BseNI ACTGG 6 cut(s) 385, 1347, 2164, 2417, 2625, 2952
BseRI GAGGAG 3 cut(s) 280, 1631, 2453
BseYI CCCAGC 2 cut(s) 2183, 2571
BsgI GTGCAG 2 cut(s) 306, 3639
Bsh1236I CGCG 2 cut(s) 3099, 4232
Bsh1285I CGRYCG 2 cut(s) 3450, 3514
BshFI GGCC 3 cut(s) 156, 670, 765
BshTI ACCGGT 1 cut(s) 1298
BshVI ATCGAT 1 cut(s) 4334
BsiEI CGRYCG 2 cut(s) 3450, 3514
BsiHKAI GWGCWC 1 cut(s) 3554
BsiHKCI CYCGRG 1 cut(s) 1027
BsiSI CCGG 5 cut(s) 162, 1299, 1374, 3514, 4252
BslFI GGGAC 5 cut(s) 452, 870, 1044, 2886, 3144
BsmAI GTCTC 8 cut(s) 583, 661, 934, 1734, 2247, 2942, 3705, 3926
BsmFI GGGAC 5 cut(s) 452, 870, 1044, 2886, 3144
BsmI GAATGC 1 cut(s) 2163
BsnI GGCC 3 cut(s) 156, 670, 765
Bso31I GGTCTC 3 cut(s) 661, 2247, 2942
BsoBI CYCGRG 1 cut(s) 1027
Bsp1286I GDGCHC 2 cut(s) 3554, 3756
Bsp1407I TGTACA 1 cut(s) 3126
BspANI GGCC 3 cut(s) 156, 670, 765
BspCNI CTCAG 7 cut(s) 34, 967, 982, 1750, 2163, 3704, 4288
BspDI ATCGAT 1 cut(s) 4334
BspFNI CGCG 2 cut(s) 3099, 4232
BspHI TCATGA 2 cut(s) 355, 904
BspLI GGNNCC 7 cut(s) 6, 489, 1509, 1941, 2749, 3171, 3267
BspMAI CTGCAG 2 cut(s) 496, 2839
BspMI ACCTGC 2 cut(s) 499, 2839
BspPI GGATC 3 cut(s) 132, 1222, 4189
BspQI GCTCTTC 6 cut(s) 1017, 1467, 1582, 1845, 2270, 3082
BspTNI GGTCTC 3 cut(s) 661, 2247, 2942
BsrBI CCGCTC 2 cut(s) 159, 2239
BsrDI GCAATG 1 cut(s) 825
BsrFI RCCGGY 2 cut(s) 1298, 1373
BsrGI TGTACA 1 cut(s) 3126
BsrI ACTGG 6 cut(s) 385, 1347, 2164, 2417, 2625, 2952
BssAI RCCGGY 2 cut(s) 1298, 1373
BssECI CCNNGG 5 cut(s) 665, 1026, 3107, 3255, 4230
BssT1I CCWWGG 1 cut(s) 665
Bst2UI CCWGG 6 cut(s) 1133, 2076, 2435, 2769, 3108, 3256
BstAPI GCANNNNNTGC 1 cut(s) 1457
BstAUI TGTACA 1 cut(s) 3126
BstBAI YACGTR 2 cut(s) 1169, 2315
BstC8I GCNNGC 4 cut(s) 759, 763, 779, 2569
BstDSI CCRYGG 1 cut(s) 4230
BstENI CCTNNNNNAGG 4 cut(s) 87, 402, 3771, 4079
BstF5I GGATG 7 cut(s) 1438, 1963, 2413, 3192, 3340, 3708, 4018
BstFNI CGCG 2 cut(s) 3099, 4232
BstHHI GCGC 4 cut(s) 1222, 1341, 2491, 3887
BstMAI GTCTC 8 cut(s) 583, 661, 934, 1734, 2247, 2942, 3705, 3926
BstMCI CGRYCG 2 cut(s) 3450, 3514
BstMWI GCNNNNNNNGC 6 cut(s) 722, 767, 1457, 3187, 4220, 4229
BstNI CCWGG 6 cut(s) 1133, 2076, 2435, 2769, 3108, 3256
BstNSI RCATGY 3 cut(s) 851, 1822, 2791
BstSCI CCNGG 7 cut(s) 1131, 2074, 2433, 2767, 3106, 3254, 3513
BstSFI CTRYAG 4 cut(s) 492, 864, 2835, 3136
BstSNI TACGTA 1 cut(s) 1169
BstUI CGCG 2 cut(s) 3099, 4232
BstV2I GAAGAC 2 cut(s) 42, 1587
BstX2I RGATCY 3 cut(s) 1227, 2495, 4279
BstXI CCANNNNNNTGG 1 cut(s) 3262
BstYI RGATCY 3 cut(s) 1227, 2495, 4279
Bsu15I ATCGAT 1 cut(s) 4334
BsuI GTATCC 1 cut(s) 1979
BsuRI GGCC 3 cut(s) 156, 670, 765
BsuTUI ATCGAT 1 cut(s) 4334
BtgI CCRYGG 1 cut(s) 4230
BtrI CACGTC 1 cut(s) 1069
BtsCI GGATG 7 cut(s) 1438, 1963, 2413, 3192, 3340, 3708, 4018
BtsI GCAGTG 1 cut(s) 2394
BtsIMutI CAGTG 5 cut(s) 1354, 2394, 2650, 2995, 3699
BveI ACCTGC 2 cut(s) 499, 2839
Cac8I GCNNGC 4 cut(s) 759, 763, 779, 2569
CaiI CAGNNNCTG 2 cut(s) 1879, 1922
CciI TCATGA 2 cut(s) 355, 904
CfoI GCGC 4 cut(s) 1222, 1341, 2491, 3887
Cfr10I RCCGGY 2 cut(s) 1298, 1373
Cfr13I GGNCC 4 cut(s) 92, 164, 662, 2828
Cfr42I CCGCGG 1 cut(s) 4233
ClaI ATCGAT 1 cut(s) 4334
CseI GACGC 5 cut(s) 2360, 2888, 3088, 3105, 3146
Csp6I GTAC 6 cut(s) 1557, 1810, 2154, 3127, 3722, 3991
CspAI ACCGGT 1 cut(s) 1298
CspCI CAANNNNNGTGG 2 cut(s) 1330, 1365
CviQI GTAC 6 cut(s) 1557, 1810, 2154, 3127, 3722, 3991
DraI TTTAAA 1 cut(s) 3564
DrdI GACNNNNNNGTC 3 cut(s) 467, 1642, 1685
DseDI GACNNNNNNGTC 3 cut(s) 467, 1642, 1685
EaeI YGGCCR 1 cut(s) 154
EciI GGCGGA 1 cut(s) 1092
Ecl136II GAGCTC 1 cut(s) 3552
Eco105I TACGTA 1 cut(s) 1169
Eco130I CCWWGG 1 cut(s) 665
Eco147I AGGCCT 1 cut(s) 765
Eco24I GRGCYC 2 cut(s) 3554, 3756
Eco31I GGTCTC 3 cut(s) 661, 2247, 2942
Eco32I GATATC 4 cut(s) 108, 426, 795, 3057
Eco47I GGWCC 4 cut(s) 92, 164, 662, 2828
Eco53kI GAGCTC 1 cut(s) 3552
Eco57I CTGAAG 6 cut(s) 72, 86, 450, 1872, 2859, 3882
Eco88I CYCGRG 1 cut(s) 1027
EcoICRI GAGCTC 1 cut(s) 3552
EcoNI CCTNNNNNAGG 4 cut(s) 87, 402, 3771, 4079
EcoO109I RGGNCCY 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 1660
EcoRII CCWGG 6 cut(s) 1131, 2074, 2433, 2767, 3106, 3254
EcoRV GATATC 4 cut(s) 108, 426, 795, 3057
EcoT14I CCWWGG 1 cut(s) 665
EcoT38I GRGCYC 2 cut(s) 3554, 3756
ErhI CCWWGG 1 cut(s) 665
FaqI GGGAC 5 cut(s) 452, 870, 1044, 2886, 3144
FauNDI CATATG 1 cut(s) 2000
FbaI TGATCA 4 cut(s) 268, 2391, 3586, 3705
FblI GTMKAC 3 cut(s) 1677, 3208, 3510
FokI GGATG 7 cut(s) 1445, 1970, 2400, 3199, 3347, 3715, 4005
FriOI GRGCYC 2 cut(s) 3554, 3756
FspBI CTAG 5 cut(s) 767, 1193, 1523, 1830, 2139
FspI TGCGCA 2 cut(s) 1340, 3886
GlaI GCGC 4 cut(s) 1221, 1340, 2490, 3886
GsaI CCCAGC 2 cut(s) 2187, 2575
GsuI CTGGAG 6 cut(s) 402, 1856, 2058, 2456, 2457, 2790
HaeIII GGCC 3 cut(s) 156, 670, 765
HapII CCGG 5 cut(s) 162, 1299, 1374, 3514, 4252
HgaI GACGC 5 cut(s) 2360, 2888, 3088, 3105, 3146
HhaI GCGC 4 cut(s) 1222, 1341, 2491, 3887
Hin6I GCGC 4 cut(s) 1220, 1339, 2489, 3885
HinP1I GCGC 4 cut(s) 1220, 1339, 2489, 3885
HincII GTYRAC 4 cut(s) 2350, 3345, 3511, 4257
HindII GTYRAC 4 cut(s) 2350, 3345, 3511, 4257
HindIII AAGCTT 2 cut(s) 98, 2519
HpaI GTTAAC 1 cut(s) 4257
HpaII CCGG 5 cut(s) 162, 1299, 1374, 3514, 4252
HphI GGTGA 9 cut(s) 1110, 1226, 1313, 2264, 3426, 3529, 3559, 3581, 3634
Hpy99I CGWCG 4 cut(s) 64, 1648, 2019, 2582
HpyCH4IV ACGT 5 cut(s) 311, 1068, 1168, 2314, 3938
HpyF10VI GCNNNNNNNGC 6 cut(s) 722, 767, 1457, 3187, 4220, 4229
HpySE526I ACGT 5 cut(s) 311, 1068, 1168, 2314, 3938
HspAI GCGC 4 cut(s) 1220, 1339, 2489, 3885
Ksp22I TGATCA 4 cut(s) 268, 2391, 3586, 3705
KspAI GTTAAC 1 cut(s) 4257
KspI CCGCGG 1 cut(s) 4233
LguI GCTCTTC 6 cut(s) 1017, 1467, 1582, 1845, 2270, 3082
LmnI GCTCC 9 cut(s) 10, 164, 184, 305, 383, 754, 1507, 2476, 2747
MaeI CTAG 5 cut(s) 767, 1193, 1523, 1830, 2139
MaeII ACGT 5 cut(s) 311, 1068, 1168, 2314, 3938
MaeIII GTNAC 9 cut(s) 1384, 2129, 2206, 2419, 2645, 2790, 3233, 3306, 4186
MbiI CCGCTC 2 cut(s) 159, 2239
MfeI CAATTG 2 cut(s) 1250, 2961
MflI RGATCY 3 cut(s) 1227, 2495, 4279
MhlI GDGCHC 2 cut(s) 3554, 3756
MluI ACGCGT 1 cut(s) 3097
MlyI GAGTC 7 cut(s) 361, 455, 917, 995, 1673, 1736, 2081
MmeI TCCRAC 4 cut(s) 804, 1417, 1798, 1997
MroXI GAANNNNTTC 1 cut(s) 1098
MslI CAYNNNNRTG 2 cut(s) 2727, 4176
MspA1I CMGCKG 2 cut(s) 113, 4232
MspI CCGG 5 cut(s) 162, 1299, 1374, 3514, 4252
MspR9I CCNGG 7 cut(s) 1133, 2076, 2435, 2769, 3108, 3256, 3515
MunI CAATTG 2 cut(s) 1250, 2961
Mva1269I GAATGC 1 cut(s) 2163
MvaI CCWGG 6 cut(s) 1133, 2076, 2435, 2769, 3108, 3256
MvnI CGCG 2 cut(s) 3099, 4232
MwoI GCNNNNNNNGC 6 cut(s) 722, 767, 1457, 3187, 4220, 4229
NciI CCSGG 1 cut(s) 3515
NdeI CATATG 1 cut(s) 2000
NlaIV GGNNCC 7 cut(s) 6, 489, 1509, 1941, 2749, 3171, 3267
NmeAIII GCCGAG 2 cut(s) 375, 3962
NmuCI GTSAC 5 cut(s) 2129, 2419, 2645, 3233, 4186
NsbI TGCGCA 2 cut(s) 1340, 3886
NspI RCATGY 3 cut(s) 851, 1822, 2791
PagI TCATGA 2 cut(s) 355, 904
PceI AGGCCT 1 cut(s) 765
PciI ACATGT 2 cut(s) 1818, 2787
PciSI GCTCTTC 6 cut(s) 1017, 1467, 1582, 1845, 2270, 3082
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PctI GAATGC 1 cut(s) 2163
PdmI GAANNNNTTC 1 cut(s) 1098
PflFI GACNNNGTC 1 cut(s) 926
PflMI CCANNNNNTGG 1 cut(s) 2434
PfoI TCCNGGA 2 cut(s) 1131, 2433
PinAI ACCGGT 1 cut(s) 1298
Ple19I CGATCG 1 cut(s) 3450
PleI GAGTC 7 cut(s) 360, 455, 916, 994, 1673, 1736, 2081
PpsI GAGTC 7 cut(s) 360, 455, 916, 994, 1673, 1736, 2081
Ppu21I YACGTR 2 cut(s) 1169, 2315
PpuMI RGGWCCY 1 cut(s) 92
PscI ACATGT 2 cut(s) 1818, 2787
Psp124BI GAGCTC 1 cut(s) 3554
Psp1406I AACGTT 1 cut(s) 3938
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 6 cut(s) 1131, 2074, 2433, 2767, 3106, 3254
PspFI CCCAGC 2 cut(s) 2183, 2571
PspGI CCWGG 6 cut(s) 1131, 2074, 2433, 2767, 3106, 3254
PspN4I GGNNCC 7 cut(s) 6, 489, 1509, 1941, 2749, 3171, 3267
PspPI GGNCC 4 cut(s) 92, 164, 662, 2828
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 2 cut(s) 496, 2839
PstNI CAGNNNCTG 2 cut(s) 1879, 1922
PsuI RGATCY 3 cut(s) 1227, 2495, 4279
PsyI GACNNNGTC 1 cut(s) 926
PvuI CGATCG 1 cut(s) 3450
RsaI GTAC 6 cut(s) 1558, 1811, 2155, 3128, 3723, 3992
RsaNI GTAC 6 cut(s) 1557, 1810, 2154, 3127, 3722, 3991
RseI CAYNNNNRTG 2 cut(s) 2727, 4176
SacI GAGCTC 1 cut(s) 3554
SacII CCGCGG 1 cut(s) 4233
SalI GTCGAC 1 cut(s) 3509
SapI GCTCTTC 6 cut(s) 1017, 1467, 1582, 1845, 2270, 3082
Sau96I GGNCC 4 cut(s) 92, 164, 662, 2828
SchI GAGTC 7 cut(s) 361, 455, 917, 995, 1673, 1736, 2081
ScrFI CCNGG 7 cut(s) 1133, 2076, 2435, 2769, 3108, 3256, 3515
SduI GDGCHC 2 cut(s) 3554, 3756
SfcI CTRYAG 4 cut(s) 492, 864, 2835, 3136
Sfr303I CCGCGG 1 cut(s) 4233
SgrBI CCGCGG 1 cut(s) 4233
SinI GGWCC 4 cut(s) 92, 164, 662, 2828
SmiMI CAYNNNNRTG 2 cut(s) 2727, 4176
SnaBI TACGTA 1 cut(s) 1169
SseBI AGGCCT 1 cut(s) 765
SspI AATATT 2 cut(s) 2941, 3420
SspMI CTAG 5 cut(s) 767, 1193, 1523, 1830, 2139
SstI GAGCTC 1 cut(s) 3554
StuI AGGCCT 1 cut(s) 765
StyD4I CCNGG 7 cut(s) 1131, 2074, 2433, 2767, 3106, 3254, 3513
StyI CCWWGG 1 cut(s) 665
TaiI ACGT 5 cut(s) 314, 1071, 1171, 2317, 3941
TaqII GACCGA 1 cut(s) 1599
TatI WGTACW 5 cut(s) 1556, 1809, 2153, 3126, 3721
TauI GCSGC 5 cut(s) 136, 139, 159, 2027, 4155
TscAI CASTG 5 cut(s) 1354, 2401, 2650, 2995, 3699
TseFI GTSAC 5 cut(s) 2129, 2419, 2645, 3233, 4186
Tsp45I GTSAC 5 cut(s) 2129, 2419, 2645, 3233, 4186
TspGWI ACGGA 3 cut(s) 1520, 1659, 2932
TspRI CASTG 5 cut(s) 1354, 2401, 2650, 2995, 3699
Tth111I GACNNNGTC 1 cut(s) 926
Van91I CCANNNNNTGG 1 cut(s) 2434
VpaK11BI GGWCC 4 cut(s) 92, 164, 662, 2828
XagI CCTNNNNNAGG 4 cut(s) 87, 402, 3771, 4079
XapI RAATTY 9 cut(s) 343, 833, 996, 1098, 1113, 1312, 1414, 1660, 2781
XceI RCATGY 3 cut(s) 851, 1822, 2791
XmiI GTMKAC 3 cut(s) 1677, 3208, 3510
XmnI GAANNNNTTC 1 cut(s) 1098
XspI CTAG 5 cut(s) 767, 1193, 1523, 1830, 2139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.