MD15G1433900.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007888
Physical Location & Seq
Forward (+)
53416524 .. 53431208
14685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1433900.v1.1.491

Sequence Viewer

Length: 2355 bp
ATGGCTATCTCTGTGGCTGAAAGAGTTTCTGATGAGCGCTGTGAACCTTCACCTGGTTCAAAAGGTTCTTTGGTTGGTTATCAAGTTCGTCTCGATAGTGCAAGCAATGACAAGACTAAGCTTCTCTTTTGTACGACGGGCATTCTTCTGAGAAAATTTGTGGGGGATAAAAACTTGACAGGAGTTACTCATGTCATAGTTGATGAAGTACATGAGCGGTCTCTTTTGGGTGATTTCCTGCTCATAGTGTTGAAGAATCTGATTGAGAAGCAATCAGCTCTTAACACACCAAAACTGAAGGTTATTCTGATGTCTGCAACTGTTGATTCAAATTTGTTTTCGAGATACTTTGGTAATTGCCCAGTGATTACTGCAGAAGGCAGAACACATCCTGTCACAACATACTATCTTGAGGATATATACGAAAGCATAGATTATCAGATTGCTTCAGACTCTCCAGCTTCCATGAGATATGGAGCTTTGACCAAAGAAAAGGCTGGTCCTGTAAACAACCGCAGGGGAAAGAAGAATCTTGTTTTATCTGGTTGGGGTGATGATTCTCTGCTCTCTGAAGAAACTATAAATCCGTATTATGTACCAGATAGTTATCAATCGTACAAAGAGAAAACTCGACAAAACTTGCAAAGAGTGAATGAAGATGTCATTGATTATGATCTTCTTGAAGACTTGGTGTGCCATGTTGATGAAACTTGCAGTGAGGGAGCCATACTTGTCTTCTTGCCTGGAGTATCTGAAATTTACACTTTAGTTGATAAGCTATCTGCTTCGTATCGATTTGGTGGACCAGCTTCTGATTGGATTCTTCCACTACATTCTTCCGTTGCATCAACTGATCAAAAGAAGGTGTTTTTACGGGCTCCTGAGAACATACGGAAGGTCATTGTAGCTACAAACATTGCAGAGACCAGCATAACAATAGATGATGTGGTATATGTTATAGACTGTGGAAAGCATAAGGAGAATCGTTATAATCCACAAAAGAAATTGTCAAGCATGGTCGAAGATTGGATATCTAGAGCAAATGCAAGGCAACGGCGAGGAAGAGCTGGACGTGTGAAACCTGGAATTTGTTTTTGCTTGTATACACGTTATCGATTTGAAAAGCTCATGCGGCCTTTTCAGGTTCCGGAGATGCTTCGAATGCCATTAGTAGAGTTGTGTTTACAAATTAAGTTGCTTTCTCTGGGTTACATAAAGTCATTTTTATCCGAGGCTTTAGAACCTCCAAGGGAAGAGGCTATGACCTCATCAATTAAGTTATTGTATGAGGTTGGTGCTCTTGAAGCAGATGAAGAGTTGACTCCTCTTGGGCATCATCTGGCAAAACTTCCCGTTGATGTATTGATTGGGAAGATGATGTTATATGGGGGAATATTTGGTTGCTTGTCGCCGATTCTCTCAATTTCAGCATTCTTGAGCTACAAATCTCCATTTATATATCCCAAAGACGAGAGACAAAATGTTGAAAGAGCTAAATTGGCACTCTTGACCGGCAAGCTAGACGGTCCAAGTGAATCAAATGATAGTGACAGGCAATCAGACCATCTTATAATGATGAGTGCTTACAGTAAATGGGAAAAGATTCTACGTGAGAAAGGGGTTAAGGCTGCGCAAAATTTTTGCAATTCATACTTCTTGAGCAGTTCCGTCATGTACATGATAAGAGACATGAGGATACAGTTTGGGACTCTGCTAGCAGTTATTGGGCTCATTGACCTTCCGAGAAAAAATAAGGTTGATGGGAGAAAGAAAGAGAATCTTGATACTTGGTTTTCAGATGCATCACAACCATTCAATATGTATTCAAACCATTCATCAATTGTAAAGGCAATATTATGTGCAGGTTTATATCCTAATGTTGCAGCCACAGAGAAAGGCATTGCTGAAGCATCTCTCAGTAACCTTAAACAATCTGCCGGTCTAGCAACTAAGGAACGCCCAATCTGGTTTGATGGAAGAAGAGAAGTTAGTATACACCCTTCTTCTATCAACAGTAATTTAAAAGAATTTCGGTACCCCTTCCTCGTCTTCCTCGAAAAGGTCGAAACCAACAAAGTGTTTCTCCGAGATACTACAGTCATTTCTCCCAACTCTATTTTGCTGTTTGGTGGCTCCATCAACATCCAGCATCAGACGGGACTTGTTATTGTTGATGGATGGTTAAAACTGACGGCGCCAGCACAAACTGCTGTTCTGTTCAAGGAACTCCGTTTAACTCTTCATTCCGTTCTGAAGGAATTGATAAGAAAGCCAGAGAATTCCACCGTTGCTCATAATGAGGTTTTAAGGTCGATTATCCATTTGTTGTTAGAAGAAGACAGGCCCCCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016282 GO:0032991 GO:0043021 GO:0043024 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0070993 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

785

Amino Acids

88.12

Weight (kDa)

8.23

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 231 - 359 1.2e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 424 - 452 1.4e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 456 - 524 5.5e-10 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 615 - 713 1.8e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 720 - 777 7.2e-17 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 990, 1571
Acc16I TGCGCA 1 cut(s) 1632
Acc36I ACCTGC 1 cut(s) 1853
Acc65I GGTACC 1 cut(s) 2034
AccB1I GGYRCC 2 cut(s) 2034, 2194
AccBSI CCGCTC 1 cut(s) 217
AccI GTMKAC 2 cut(s) 1103, 1993
AccIII TCCGGA 1 cut(s) 1147
AciI CCGC 3 cut(s) 217, 514, 1132
AcsI RAATTY 7 cut(s) 155, 331, 756, 1086, 1636, 2027, 2278
AcuI CTGAAG 5 cut(s) 317, 432, 591, 1926, 2273
AcyI GRCGYC 1 cut(s) 2195
AfaI GTAC 6 cut(s) 133, 210, 597, 617, 1676, 2036
AfeI AGCGCT 1 cut(s) 38
AfiI CCNNNNNNNGG 2 cut(s) 53, 2059
AflIII ACRYGT 2 cut(s) 1072, 1106
AjiI CACGTC 1 cut(s) 1073
AjnI CCWGG 3 cut(s) 52, 742, 1081
Alw21I GWGCWC 1 cut(s) 1300
Alw26I GTCTC 5 cut(s) 95, 225, 917, 1468, 1680
AlwNI CAGNNNCTG 1 cut(s) 812
Aor13HI TCCGGA 1 cut(s) 1147
Aor51HI AGCGCT 1 cut(s) 38
AoxI GGCC 2 cut(s) 1133, 2342
ApeKI GCWGC 2 cut(s) 1628, 1883
ApoI RAATTY 7 cut(s) 155, 331, 756, 1086, 1636, 2027, 2278
Asp700I GAANNNNTTC 1 cut(s) 1602
Asp718I GGTACC 1 cut(s) 2034
AspLEI GCGC 3 cut(s) 39, 1633, 2197
AspS9I GGNCC 4 cut(s) 500, 803, 1526, 2343
AsuHPI GGTGA 3 cut(s) 42, 242, 563
AsuII TTCGAA 1 cut(s) 1159
AsuNHI GCTAGC 1 cut(s) 1714
AvaII GGWCC 3 cut(s) 500, 803, 1526
BaeI ACNNNNGTAYC 4 cut(s) 337, 370, 2082, 2115
BanI GGYRCC 2 cut(s) 2034, 2194
BanII GRGCYC 2 cut(s) 880, 1731
BarI GAAGNNNNNNTAC 4 cut(s) 1977, 1984, 2009, 2016
BbsI GAAGAC 4 cut(s) 690, 727, 2041, 2343
Bbv12I GWGCWC 1 cut(s) 1300
BbvI GCAGC 2 cut(s) 1615, 1895
BccI CCATC 6 cut(s) 1572, 1754, 1967, 2144, 2168, 2172
BceAI ACGGC 2 cut(s) 1070, 2208
BciT130I CCWGG 3 cut(s) 54, 744, 1083
BciVI GTATCC 1 cut(s) 1689
BclI TGATCA 1 cut(s) 853
BcoDI GTCTC 5 cut(s) 95, 225, 917, 1468, 1680
BfaI CTAG 4 cut(s) 1035, 1520, 1715, 1943
BfmI CTRYAG 2 cut(s) 372, 2094
BfoI RGCGCY 2 cut(s) 40, 2198
BfuAI ACCTGC 1 cut(s) 1853
BfuI GTATCC 1 cut(s) 1689
BisI GCNGC 3 cut(s) 1133, 1629, 1884
BlsI GCNGC 3 cut(s) 1134, 1630, 1885
Bme1390I CCNGG 3 cut(s) 54, 744, 1083
Bme18I GGWCC 3 cut(s) 500, 803, 1526
BmgBI CACGTC 1 cut(s) 1073
BmgT120I GGNCC 4 cut(s) 500, 803, 1526, 2343
BmiI GGNNCC 7 cut(s) 724, 879, 1146, 2036, 2134, 2196, 2345
BmrFI CCNGG 3 cut(s) 54, 744, 1083
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 7 cut(s) 854, 1143, 1342, 1789, 1811, 1919, 2158
BmtI GCTAGC 1 cut(s) 1718
BmuI ACTGGG 1 cut(s) 356
BpiI GAAGAC 4 cut(s) 690, 727, 2041, 2343
BpmI CTGGAG 2 cut(s) 441, 765
Bpu14I TTCGAA 1 cut(s) 1159
BpuEI CTTGAG 3 cut(s) 431, 1456, 1678
Bsa29I ATCGAT 2 cut(s) 793, 1114
BsaAI YACGTR 1 cut(s) 1610
BsaBI GATNNNNATC 2 cut(s) 606, 672
BsaHI GRCGYC 1 cut(s) 2195
BsaI GGTCTC 2 cut(s) 225, 917
BsaJI CCNNGG 2 cut(s) 1230, 1247
BsaWI WCCGGW 1 cut(s) 1147
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 2059
Bse118I RCCGGY 2 cut(s) 1511, 1937
Bse1I ACTGG 1 cut(s) 362
Bse3DI GCAATG 3 cut(s) 112, 915, 1899
Bse8I GATNNNNATC 2 cut(s) 606, 672
BseAI TCCGGA 1 cut(s) 1147
BseBI CCWGG 3 cut(s) 54, 744, 1083
BseCI ATCGAT 2 cut(s) 793, 1114
BseDI CCNNGG 2 cut(s) 1230, 1247
BseGI GGATG 3 cut(s) 388, 2142, 2183
BseJI GATNNNNATC 2 cut(s) 606, 672
BseLI CCNNNNNNNGG 2 cut(s) 53, 2059
BseMI GCAATG 3 cut(s) 112, 915, 1899
BseMII CTCAG 3 cut(s) 140, 873, 1930
BseNI ACTGG 1 cut(s) 362
BseRI GAGGAG 1 cut(s) 1314
BseXI GCAGC 2 cut(s) 1615, 1895
BsgI GTGCAG 1 cut(s) 1881
BshFI GGCC 2 cut(s) 1135, 2344
BshNI GGYRCC 2 cut(s) 2034, 2194
BshVI ATCGAT 2 cut(s) 793, 1114
BsiHKAI GWGCWC 1 cut(s) 1300
BsiSI CCGG 3 cut(s) 1148, 1512, 1938
BslFI GGGAC 2 cut(s) 1720, 2172
BslI CCNNNNNNNGG 2 cut(s) 53, 2059
BsmAI GTCTC 5 cut(s) 95, 225, 917, 1468, 1680
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 2 cut(s) 1720, 2172
BsmI GAATGC 3 cut(s) 141, 1167, 1430
BsnI GGCC 2 cut(s) 1135, 2344
Bso31I GGTCTC 2 cut(s) 225, 917
Bsp119I TTCGAA 1 cut(s) 1159
Bsp1286I GDGCHC 3 cut(s) 880, 1300, 1731
Bsp13I TCCGGA 1 cut(s) 1147
Bsp1407I TGTACA 1 cut(s) 1674
Bsp143I GATC 2 cut(s) 673, 853
BspACI CCGC 3 cut(s) 217, 514, 1132
BspANI GGCC 2 cut(s) 1135, 2344
BspCNI CTCAG 3 cut(s) 141, 874, 1929
BspDI ATCGAT 2 cut(s) 793, 1114
BspEI TCCGGA 1 cut(s) 1147
BspLI GGNNCC 7 cut(s) 724, 879, 1146, 2036, 2134, 2196, 2345
BspMAI CTGCAG 1 cut(s) 376
BspMI ACCTGC 1 cut(s) 1853
BspOI GCTAGC 1 cut(s) 1718
BspQI GCTCTTC 1 cut(s) 1057
BspT104I TTCGAA 1 cut(s) 1159
BspT107I GGYRCC 2 cut(s) 2034, 2194
BspTNI GGTCTC 2 cut(s) 225, 917
BsrBI CCGCTC 1 cut(s) 217
BsrDI GCAATG 3 cut(s) 112, 915, 1899
BsrFI RCCGGY 2 cut(s) 1511, 1937
BsrGI TGTACA 1 cut(s) 1674
BsrI ACTGG 1 cut(s) 362
BssAI RCCGGY 2 cut(s) 1511, 1937
BssECI CCNNGG 2 cut(s) 1230, 1247
BssMI GATC 2 cut(s) 673, 853
BssNAI GTATAC 2 cut(s) 1104, 1994
BssNI GRCGYC 1 cut(s) 2195
BssT1I CCWWGG 1 cut(s) 1247
Bst1107I GTATAC 2 cut(s) 1104, 1994
Bst2UI CCWGG 3 cut(s) 54, 744, 1083
Bst4CI ACNGT 8 cut(s) 322, 965, 1526, 1589, 1701, 2015, 2098, 2287
Bst6I CTCTTC 5 cut(s) 1057, 1248, 1308, 1975, 2244
BstACI GRCGYC 1 cut(s) 2195
BstAPI GCANNNNNTGC 1 cut(s) 2207
BstAUI TGTACA 1 cut(s) 1674
BstBAI YACGTR 1 cut(s) 1610
BstBI TTCGAA 1 cut(s) 1159
BstC8I GCNNGC 4 cut(s) 103, 1517, 1716, 2199
BstDEI CTNAG 5 cut(s) 117, 149, 882, 1916, 1950
BstENI CCTNNNNNAGG 1 cut(s) 2057
BstF5I GGATG 3 cut(s) 388, 2142, 2183
BstH2I RGCGCY 2 cut(s) 40, 2198
BstHHI GCGC 3 cut(s) 39, 1633, 2197
BstKTI GATC 2 cut(s) 676, 856
BstMAI GTCTC 5 cut(s) 95, 225, 917, 1468, 1680
BstMBI GATC 2 cut(s) 673, 853
BstMWI GCNNNNNNNGC 6 cut(s) 1132, 1162, 1304, 1499, 1943, 2207
BstNI CCWGG 3 cut(s) 54, 744, 1083
BstSCI CCNGG 3 cut(s) 52, 742, 1081
BstSFI CTRYAG 2 cut(s) 372, 2094
BstV1I GCAGC 2 cut(s) 1615, 1895
BstV2I GAAGAC 4 cut(s) 690, 727, 2041, 2343
BstZ17I GTATAC 2 cut(s) 1104, 1994
Bsu15I ATCGAT 2 cut(s) 793, 1114
BsuI GTATCC 1 cut(s) 1689
BsuRI GGCC 2 cut(s) 1135, 2344
BsuTUI ATCGAT 2 cut(s) 793, 1114
BtrI CACGTC 1 cut(s) 1073
BtsCI GGATG 3 cut(s) 388, 2142, 2183
BtsI GCAGTG 1 cut(s) 721
BtsIMutI CAGTG 2 cut(s) 369, 721
BveI ACCTGC 1 cut(s) 1853
Cac8I GCNNGC 4 cut(s) 103, 1517, 1716, 2199
CaiI CAGNNNCTG 1 cut(s) 812
CfoI GCGC 3 cut(s) 39, 1633, 2197
Cfr10I RCCGGY 2 cut(s) 1511, 1937
Cfr13I GGNCC 4 cut(s) 500, 803, 1526, 2343
ClaI ATCGAT 2 cut(s) 793, 1114
CsiI ACCWGGT 1 cut(s) 52
Csp6I GTAC 6 cut(s) 132, 209, 596, 616, 1675, 2035
CviAII CATG 9 cut(s) 191, 212, 466, 698, 1015, 1129, 1672, 1678, 1690
CviQI GTAC 6 cut(s) 132, 209, 596, 616, 1675, 2035
DdeI CTNAG 5 cut(s) 117, 149, 882, 1916, 1950
DinI GGCGCC 1 cut(s) 2196
DpnI GATC 2 cut(s) 675, 855
DpnII GATC 2 cut(s) 673, 853
DraI TTTAAA 1 cut(s) 2022
Eam1104I CTCTTC 5 cut(s) 1057, 1248, 1308, 1975, 2244
EarI CTCTTC 5 cut(s) 1057, 1248, 1308, 1975, 2244
Eco130I CCWWGG 1 cut(s) 1247
Eco24I GRGCYC 2 cut(s) 880, 1731
Eco31I GGTCTC 2 cut(s) 225, 917
Eco32I GATATC 1 cut(s) 1032
Eco47I GGWCC 3 cut(s) 500, 803, 1526
Eco47III AGCGCT 1 cut(s) 38
Eco57I CTGAAG 5 cut(s) 317, 432, 591, 1926, 2273
EcoNI CCTNNNNNAGG 1 cut(s) 2057
EcoO109I RGGNCCY 1 cut(s) 2343
EcoRI GAATTC 1 cut(s) 2278
EcoRII CCWGG 3 cut(s) 52, 742, 1081
EcoRV GATATC 1 cut(s) 1032
EcoT14I CCWWGG 1 cut(s) 1247
EcoT22I ATGCAT 1 cut(s) 1804
EcoT38I GRGCYC 2 cut(s) 880, 1731
EgeI GGCGCC 1 cut(s) 2196
EheI GGCGCC 1 cut(s) 2196
ErhI CCWWGG 1 cut(s) 1247
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 9 cut(s) 194, 215, 469, 701, 1018, 1132, 1675, 1681, 1693
FalI AAGNNNNNCTT 4 cut(s) 110, 142, 1764, 1796
FaqI GGGAC 2 cut(s) 1720, 2172
FatI CATG 9 cut(s) 190, 211, 465, 697, 1014, 1128, 1671, 1677, 1689
FbaI TGATCA 1 cut(s) 853
FblI GTMKAC 2 cut(s) 1103, 1993
Fnu4HI GCNGC 3 cut(s) 1133, 1629, 1884
FokI GGATG 3 cut(s) 375, 2129, 2190
FriOI GRGCYC 2 cut(s) 880, 1731
Fsp4HI GCNGC 3 cut(s) 1133, 1629, 1884
FspBI CTAG 4 cut(s) 1035, 1520, 1715, 1943
FspI TGCGCA 1 cut(s) 1632
GlaI GCGC 3 cut(s) 38, 1632, 2196
GluI GCNGC 3 cut(s) 1133, 1629, 1884
GsuI CTGGAG 2 cut(s) 441, 765
HaeII RGCGCY 2 cut(s) 40, 2198
HaeIII GGCC 2 cut(s) 1135, 2344
HapII CCGG 3 cut(s) 1148, 1512, 1938
HhaI GCGC 3 cut(s) 39, 1633, 2197
Hin1I GRCGYC 1 cut(s) 2195
Hin1II CATG 9 cut(s) 194, 215, 469, 701, 1018, 1132, 1675, 1681, 1693
Hin6I GCGC 3 cut(s) 37, 1631, 2195
HinP1I GCGC 3 cut(s) 37, 1631, 2195
HincII GTYRAC 1 cut(s) 1320
HindII GTYRAC 1 cut(s) 1320
HindIII AAGCTT 1 cut(s) 119
HpaII CCGG 3 cut(s) 1148, 1512, 1938
HphI GGTGA 3 cut(s) 42, 242, 563
Hpy166II GTNNAC 7 cut(s) 44, 508, 803, 1104, 1184, 1320, 1994
Hpy8I GTNNAC 7 cut(s) 44, 508, 803, 1104, 1184, 1320, 1994
Hpy99I CGWCG 1 cut(s) 139
HpyAV CCTTC 9 cut(s) 57, 292, 371, 856, 889, 1748, 2010, 2050, 2248
HpyCH4III ACNGT 8 cut(s) 322, 965, 1526, 1589, 1701, 2015, 2098, 2287
HpyCH4IV ACGT 3 cut(s) 1072, 1108, 1609
HpyF10VI GCNNNNNNNGC 6 cut(s) 1132, 1162, 1304, 1499, 1943, 2207
HpyF3I CTNAG 5 cut(s) 117, 149, 882, 1916, 1950
HpySE526I ACGT 3 cut(s) 1072, 1108, 1609
Hsp92I GRCGYC 1 cut(s) 2195
Hsp92II CATG 9 cut(s) 194, 215, 469, 701, 1018, 1132, 1675, 1681, 1693
HspAI GCGC 3 cut(s) 37, 1631, 2195
KasI GGCGCC 1 cut(s) 2194
Kpn2I TCCGGA 1 cut(s) 1147
KpnI GGTACC 1 cut(s) 2038
Ksp22I TGATCA 1 cut(s) 853
Kzo9I GATC 2 cut(s) 673, 853
LguI GCTCTTC 1 cut(s) 1057
LmnI GCTCC 4 cut(s) 476, 722, 883, 2138
Lsp1109I GCAGC 2 cut(s) 1615, 1895
LweI GCATC 7 cut(s) 854, 1143, 1342, 1789, 1811, 1919, 2158
MabI ACCWGGT 1 cut(s) 52
MaeI CTAG 4 cut(s) 1035, 1520, 1715, 1943
MaeII ACGT 3 cut(s) 1072, 1108, 1609
MaeIII GTNAC 5 cut(s) 184, 394, 1208, 1547, 1919
MalI GATC 2 cut(s) 675, 855
MbiI CCGCTC 1 cut(s) 217
MboI GATC 2 cut(s) 673, 853
MfeI CAATTG 1 cut(s) 1839
MhlI GDGCHC 3 cut(s) 880, 1300, 1731
Mly113I GGCGCC 1 cut(s) 2195
MlyI GAGTC 3 cut(s) 446, 1315, 1702
Mph1103I ATGCAT 1 cut(s) 1804
MroI TCCGGA 1 cut(s) 1147
MroXI GAANNNNTTC 1 cut(s) 1602
MseI TTAA 9 cut(s) 282, 1191, 1275, 1623, 1926, 2021, 2183, 2234, 2306
MslI CAYNNNNRTG 2 cut(s) 702, 1676
MspI CCGG 3 cut(s) 1148, 1512, 1938
MspR9I CCNGG 3 cut(s) 54, 744, 1083
MunI CAATTG 1 cut(s) 1839
Mva1269I GAATGC 3 cut(s) 141, 1167, 1430
MvaI CCWGG 3 cut(s) 54, 744, 1083
MwoI GCNNNNNNNGC 6 cut(s) 1132, 1162, 1304, 1499, 1943, 2207
NarI GGCGCC 1 cut(s) 2195
NdeII GATC 2 cut(s) 673, 853
NheI GCTAGC 1 cut(s) 1714
NlaIII CATG 9 cut(s) 194, 215, 469, 701, 1018, 1132, 1675, 1681, 1693
NlaIV GGNNCC 7 cut(s) 724, 879, 1146, 2036, 2134, 2196, 2345
NmuCI GTSAC 2 cut(s) 394, 1547
NsbI TGCGCA 1 cut(s) 1632
NsiI ATGCAT 1 cut(s) 1804
NspV TTCGAA 1 cut(s) 1159
PciSI GCTCTTC 1 cut(s) 1057
PcsI WCGNNNNNNNCGW 2 cut(s) 2052, 2061
PctI GAATGC 3 cut(s) 141, 1167, 1430
PdmI GAANNNNTTC 1 cut(s) 1602
PkrI GCNGC 3 cut(s) 1134, 1630, 1885
PleI GAGTC 3 cut(s) 446, 1315, 1702
PluTI GGCGCC 1 cut(s) 2198
PpsI GAGTC 3 cut(s) 446, 1315, 1702
Ppu21I YACGTR 1 cut(s) 1610
PsiI TTATAA 2 cut(s) 990, 1571
Psp6I CCWGG 3 cut(s) 52, 742, 1081
PspGI CCWGG 3 cut(s) 52, 742, 1081
PspN4I GGNNCC 7 cut(s) 724, 879, 1146, 2036, 2134, 2196, 2345
PspPI GGNCC 4 cut(s) 500, 803, 1526, 2343
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 1 cut(s) 812
RsaI GTAC 6 cut(s) 133, 210, 597, 617, 1676, 2036
RsaNI GTAC 6 cut(s) 132, 209, 596, 616, 1675, 2035
RseI CAYNNNNRTG 2 cut(s) 702, 1676
SapI GCTCTTC 1 cut(s) 1057
SaqAI TTAA 9 cut(s) 282, 1191, 1275, 1623, 1926, 2021, 2183, 2234, 2306
SatI GCNGC 3 cut(s) 1133, 1629, 1884
Sau3AI GATC 2 cut(s) 673, 853
Sau96I GGNCC 4 cut(s) 500, 803, 1526, 2343
SchI GAGTC 3 cut(s) 446, 1315, 1702
ScrFI CCNGG 3 cut(s) 54, 744, 1083
SduI GDGCHC 3 cut(s) 880, 1300, 1731
SexAI ACCWGGT 1 cut(s) 52
SfaNI GCATC 7 cut(s) 854, 1143, 1342, 1789, 1811, 1919, 2158
SfcI CTRYAG 2 cut(s) 372, 2094
SfoI GGCGCC 1 cut(s) 2196
SfuI TTCGAA 1 cut(s) 1159
SinI GGWCC 3 cut(s) 500, 803, 1526
SmiMI CAYNNNNRTG 2 cut(s) 702, 1676
SmlI CTYRAG 3 cut(s) 410, 1435, 1657
SmoI CTYRAG 3 cut(s) 410, 1435, 1657
SsiI CCGC 3 cut(s) 217, 514, 1132
SspDI GGCGCC 1 cut(s) 2194
SspI AATATT 2 cut(s) 1395, 1854
SspMI CTAG 4 cut(s) 1035, 1520, 1715, 1943
StyD4I CCNGG 3 cut(s) 52, 742, 1081
StyI CCWWGG 1 cut(s) 1247
TaaI ACNGT 8 cut(s) 322, 965, 1526, 1589, 1701, 2015, 2098, 2287
TaiI ACGT 3 cut(s) 1075, 1111, 1612
TatI WGTACW 2 cut(s) 208, 1674
TauI GCSGC 1 cut(s) 1135
Tru1I TTAA 9 cut(s) 282, 1191, 1275, 1623, 1926, 2021, 2183, 2234, 2306
Tru9I TTAA 9 cut(s) 282, 1191, 1275, 1623, 1926, 2021, 2183, 2234, 2306
TscAI CASTG 2 cut(s) 369, 721
TseFI GTSAC 2 cut(s) 394, 1547
TseI GCWGC 2 cut(s) 1628, 1883
Tsp45I GTSAC 2 cut(s) 394, 1547
TspDTI ATGAA 7 cut(s) 219, 669, 720, 1326, 1638, 1824, 2231
TspGWI ACGGA 6 cut(s) 576, 829, 907, 1657, 2219, 2236
TspRI CASTG 2 cut(s) 369, 721
VpaK11BI GGWCC 3 cut(s) 500, 803, 1526
XagI CCTNNNNNAGG 1 cut(s) 2057
XapI RAATTY 7 cut(s) 155, 331, 756, 1086, 1636, 2027, 2278
XbaI TCTAGA 1 cut(s) 1034
XcmI CCANNNNNNNNNTGG 1 cut(s) 813
XmiI GTMKAC 2 cut(s) 1103, 1993
XmnI GAANNNNTTC 1 cut(s) 1602
XspI CTAG 4 cut(s) 1035, 1520, 1715, 1943
Zsp2I ATGCAT 1 cut(s) 1804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.