RLG00000003172

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007888
Physical Location & Seq
Forward (+)
45217705 .. 45223601
5897 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003172

Sequence Viewer

Length: 3699 bp
ATGGGTGCTAAGGTCAGTAAACCAAGGTCTTATGATGTTATAGCCAAAGAGTACCACGCTGCGCGGTTGGAAGCTGCAGAAGCCAAGCAACAAAAGGACAAGAAAAACCAGGAACGGGCAGGCAAAATGATACGTGATCTCAAGCAAGAGTTGGCTGCTCTAGGCATATCGGATGACATCTTGGCATCAGAATTTGAGCAGGAACAGTCGATAGAACGAGCTTATTCTGCTTTTGAGGACACAGATGCTAGTTCACAGCCTTACAAGCAGTTTGAAGGACTCCATGCTGATGAATTAAAAGTAGACGGTAATGATATGGAACACTGCACCTCCATTCAGCTCCCAATCAACTCTACTCCTTCAGATTTACCTGTTCAGGAAAAACTTGTTGCAGAAGAAGAGACAACTGATATGGAAATTAGTAATTTTTTCTTGGAAGATGCACCATCTAACGAATTCCTAACTCCTACTATTTTGGAGCTACAGAAGAAAGAAAAGTTGAGAGAAATGCTTAGTGAGAAAAATTTGGAGAAATTAGATGGTATTTGGAAGAAGGGGGATCCTCAAAAGATTCCCAAGGCTATTTTTCAGCAATTATGTCAAAAATCAGGATGGGAAGCCCCAAAGTTCAATAAAGTGCGGGGAAAGGATAATAGTTTCTCTTATACTATCAGTGTTTTGCGTAAAGCTAGTGGAAGGGGTAAGAGCAGAAAGGCTGGGGGCTTGGTCACTCTTCAGCTTCCTGATCACGATGTAACTTTTGATTCAGCTGAGGATGCACAGAATAGAGTGGCAGCATATGCTCTTTGTCAACTGTTTCCTGATCTCCCAATCCATCTTGTAATTACTGAGCCCTATGCTTCTCTAGTTGTTCAATGGAAGGAAGGGGAAACAATAACCAATGTAGAAGATAGTGTTAAGGACCGAAGAGCTACTTTTGTGGATTCACTATTGAAAGCTGATGGTTCTACTTCAACATCTGCTGTTGTATATGATTCTGATTCTCTTCCTAAAGTGGTTCCACAACTCCAGGTTCAAGAACCTAGAAATGCAGAACTAAATCCTCGCAAAGATGCAGAGAGCTCTTATTTGAAACAAGAACTAGAGAATAAACAGAAAAAGCAGAAATTCAAGGAAATGTTGAGAGCTAGAGCTGCACTCCCCATTGCTGGTTTGAAGGGTGATATATTGCAACTGTTGCAGGATAATAATGTTCTAGTAGTTTGTGGGGAAACGGGATCTGGAAAAACAACTCAGGTTCCACAATTTATATTGGATGACATGATCCAGTCAGGACGTGGTGGACACTGTAACATTATATGCACACAACCAAGGAGAATAGCAGCTATTTCTGTGGCTGACAGAGTCGCTGATGAGCGTTGTGAACCTTCACCTGGTTCAAATGGTTCTTTGGTTGGTTATCAAGTACGCCTTGATAATGCAAGTAATGAAAAGACTAAGCTTCTTTTTTGCACAACAGGTATTCTTCTGAGAAAATTCGTGGGAGACAGAAACTTGACTGGAATTACTCATGTCATAGTTGATGAAGTACATGAGCGGTCTCTTTTGGGTGATTTCCTGCTCATAGTTTTGAAGAATCTGATTGAGAAGCAATCAGCTCTTAACACACCAAAACTGAAAGTTATTCTAATGTCTGCAACTGTTGATTCCAATTTGTTTTCAAATTACTTTGGTGGTTGTCCTGTGATTACTGCAGAAGGTAGAACACATCCAGTGACGACTTATTTTCTTGAGGATATTTATGAGCGCATTGATTATCGGCTTGCATCAGATTCTCCGGCTTCCATGGTCTATGGAACTTCGACCGAAGGGAAGACTGGTCCTGTAGACAACAGCAGAGGAAAGAAGAATCTTGTTTTATCTGGCTGGGGTGATGATTCTCTACTCTCTGAAGAATATATTAATCCAAATTATGTTCATGATACGTATCAATCTTACAGAGAGCAAACTCGACAAAATTTGAAAAGATTGAATGAAGATGTCATTGATTATGATCTTCTTGAAGACTTGGTGTGCCATGTTGATGAAACTTGCGGCGAAGGTGCTATTCTTGTCTTCTTGCCTGGAGTATCTGAAATTTACACCTTAGTTGATAAACTAGCTGCTTCTTACCGGTTTGGTGGAGCAGCTTCTGATTGGATCCTCCCGCTACATTCATCTGTTGCCTCAGTTGATCAAAAGAAGGTGTTTTTGCAGGCTCCTGAGAACGTACGAAAGATTATTGTAGCCACTAATATTGCTGAGACCAGCATAACAATAGATGATGTGGTGTATGTTATTGACTGTGGAAAGCATAAGGAGAATCGTTATAATCCTCAGAAGAAATTGTCGAGCATGGTTGAGGATTGGATTTCTAAAGCAAATGCAAGGCAACGTCGAGGACGAGCTGGCCGTGTGAAACCTGGAATTTGCTTTTGCCTGTACACTAGTTATCGATTTGAAAAGCTGATGCGCCCCTTTCAGGTTCCAGAGATGCTTCGAATGCCATTAGTAGAGTTGTGTTTACAAATTAAGTTACTTTCTCTGGGCCACATAAAACCATTTTTGTCCCAGGCTTTGGAACCTCCAAGGGAAGAGGCTATGACTTCAGCAATTAAGATATTGTATGAGGTTGGTGCTCTTGAAACGGATGAAGAGTTAACGCCTCTTGGGCATCATTTGGCGAAACTTCCTGTTGACGTATTGATTGGGAAGATGATGATATATGGTGGAATATTTGGTTGCTTATCACCCATTCTATCAATTTCAGCATTCTTAAGTTACAAATCTCCATTTGTCCATCCAAAAGATGAGAAGGAAAATGCTAAAAGAGCAAAATTGGCACTGTTGACTGACAAGCTAGATGGTCCAAGTGAATCAAATGATGTTGACAAGCAATCCGACCATCTCATAATGATAACTGCATACAAGAAATGGGAAAAGATTCTACGTGACAAAGGGGTTAGAGCTGCGCAGCAGTTTTGCAATTCATATTATTTGAGCAGTTCAGTGATGTACATGATAAGAGACATGAGGATACAGTTTGGAACACTGCTTGCTGATATTGGACTCATTGATCTTCCAAAGAAATATCAGGTTGATGGAAGAAAGAAAGAGAATCTTGACGCTTGGTTTTCGGATGCATCGCAACCCTTCAATATGTATTCAAACCATTCACCAATTGTAAAAGCTGTCATATGTGCAGGTTTATACCCTAACATTGCAGCCACTGAGAAAGGCATTGCTGGAACAGTCCTCAATAACCTTAAACAGGCTCCTGGTCACGCAACTAGCCATTGTCCAACCTGGTATGATGGAAGAAGAAAAGTCAATATACACCCTTCTTCTATCAACCATAATGTAAATGAATTTCGGTATCCTTTCCTTGTCTTCCTCGAAAAGGTTGAAACCAACAAAGTATTTTTGCGGGACTCTACAATCATTTCTCCCAACTCTATTTTGCTGTTTGGTGGCTCCATTAACATCCAGCATCAGACTGGACTTGTTATTGTTGATGGATGGTTAAAACTGACAGCACCAGCACAGACTGCTGTCCTGTTCAAGGAACTCCGTTTAACTCTTCACTCTGTTCTGAAAGAATTGATAAGAAAGCCTGAGAATTGCACTGTTGATCATAATGAGGTTTTAAGGTCTATTATCCATTTGTTGTTGGAAGAAGACAAGCCGCCTCCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016282 GO:0032991 GO:0043021 GO:0043024 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0070993 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1233

Amino Acids

137.96

Weight (kDa)

6.25

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 398 - 558 1.6e-10 DEAD/DEAH box helicase
Helicase_C PF00271 683 - 806 7.3e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 871 - 899 2.3e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 903 - 971 2.6e-10 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1062 - 1160 2.6e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1167 - 1224 5.7e-16 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2331
Acc16I TGCGCA 1 cut(s) 2973
Acc36I ACCTGC 1 cut(s) 3194
AccB7I CCANNNNNTGG 1 cut(s) 2578
AccBSI CCGCTC 1 cut(s) 1558
AccI GTMKAC 2 cut(s) 303, 1848
AccII CGCG 1 cut(s) 64
AciI CCGC 7 cut(s) 64, 640, 1558, 2055, 2168, 3427, 3686
AclWI GGATC 6 cut(s) 554, 567, 1246, 1279, 2155, 2168
AcoI YGGCCR 1 cut(s) 2410
AcsI RAATTY 9 cut(s) 191, 455, 523, 1127, 1496, 1978, 2097, 2427, 3368
AcuI CTGAAG 4 cut(s) 345, 719, 1932, 2592
AfaI GTAC 6 cut(s) 53, 1428, 1551, 2232, 2444, 3017
AfiI CCNNNNNNNGG 8 cut(s) 115, 1169, 1394, 2482, 2578, 3271, 3400, 3562
AflII CTTAAG 1 cut(s) 2776
AgeI ACCGGT 1 cut(s) 2133
AhlI ACTAGT 1 cut(s) 2447
AjiI CACGTC 1 cut(s) 1298
AjnI CCWGG 8 cut(s) 108, 1029, 1393, 2083, 2422, 2571, 3277, 3305
Alw21I GWGCWC 2 cut(s) 1085, 2641
Alw26I GTCTC 5 cut(s) 395, 1500, 1566, 2258, 3021
AlwI GGATC 6 cut(s) 554, 567, 1246, 1279, 2155, 2168
AlwNI CAGNNNCTG 2 cut(s) 2153, 3230
AoxI GGCC 2 cut(s) 2410, 2548
ApoI RAATTY 9 cut(s) 191, 455, 523, 1127, 1496, 1978, 2097, 2427, 3368
AseI ATTAAT 1 cut(s) 1923
AsiGI ACCGGT 1 cut(s) 2133
Asp700I GAANNNNTTC 1 cut(s) 2943
AspLEI GCGC 4 cut(s) 64, 1770, 2475, 2974
AspS9I GGNCC 4 cut(s) 922, 1841, 2548, 2867
AsuHPI GGTGA 6 cut(s) 1193, 1383, 1583, 1904, 2742, 3168
AsuII TTCGAA 1 cut(s) 2500
AvaII GGWCC 3 cut(s) 922, 1841, 2867
BamHI GGATCC 2 cut(s) 559, 2160
BanII GRGCYC 2 cut(s) 855, 1085
BbsI GAAGAC 5 cut(s) 1841, 2031, 2068, 3382, 3684
Bbv12I GWGCWC 2 cut(s) 1085, 2641
BbvCI CCTCAGC 1 cut(s) 771
BceAI ACGGC 1 cut(s) 2397
BciT130I CCWGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
BciVI GTATCC 2 cut(s) 3030, 3387
BclI TGATCA 3 cut(s) 745, 2194, 3631
BcoDI GTCTC 5 cut(s) 395, 1500, 1566, 2258, 3021
BcuI ACTAGT 1 cut(s) 2447
BfmI CTRYAG 4 cut(s) 75, 482, 1713, 1845
BfrI CTTAAG 1 cut(s) 2776
BfuAI ACCTGC 1 cut(s) 3194
BfuI GTATCC 2 cut(s) 3030, 3387
BglI GCCNNNNNGGC 1 cut(s) 2671
Bme1390I CCNGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
Bme18I GGWCC 3 cut(s) 922, 1841, 2867
BmgBI CACGTC 1 cut(s) 1298
BmgT120I GGNCC 4 cut(s) 922, 1841, 2548, 2867
BmiI GGNNCC 9 cut(s) 561, 1020, 1260, 2162, 2220, 2487, 2583, 3276, 3475
BmrFI CCNGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
BoxI GACNNNNGTC 1 cut(s) 3551
BpiI GAAGAC 5 cut(s) 1841, 2031, 2068, 3382, 3684
BplI GAGNNNNNCTC 2 cut(s) 1143, 1175
BpmI CTGGAG 2 cut(s) 1013, 2106
Bpu10I CCTNAGC 2 cut(s) 9, 771
Bpu14I TTCGAA 1 cut(s) 2500
BpuEI CTTGAG 2 cut(s) 125, 1772
Bsa29I ATCGAT 1 cut(s) 2455
BsaAI YACGTR 3 cut(s) 134, 1947, 2951
BsaBI GATNNNNATC 2 cut(s) 1947, 2013
BsaI GGTCTC 2 cut(s) 1566, 2258
BsaJI CCNNGG 6 cut(s) 23, 576, 1331, 1806, 2571, 2588
BsaWI WCCGGW 1 cut(s) 2133
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 8 cut(s) 115, 1169, 1394, 2482, 2578, 3271, 3400, 3562
Bse118I RCCGGY 1 cut(s) 2133
Bse1I ACTGG 5 cut(s) 1288, 1525, 1733, 1843, 3502
Bse3DI GCAATG 3 cut(s) 1164, 3219, 3240
Bse8I GATNNNNATC 2 cut(s) 1947, 2013
BseBI CCWGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
BseCI ATCGAT 1 cut(s) 2455
BseDI CCNNGG 6 cut(s) 23, 576, 1331, 1806, 2571, 2588
BseJI GATNNNNATC 2 cut(s) 1947, 2013
BseLI CCNNNNNNNGG 8 cut(s) 115, 1169, 1394, 2482, 2578, 3271, 3400, 3562
BseMI GCAATG 3 cut(s) 1164, 3219, 3240
BseNI ACTGG 5 cut(s) 1288, 1525, 1733, 1843, 3502
BseRI GAGGAG 1 cut(s) 3681
BseYI CCCAGC 2 cut(s) 716, 1887
BsgI GTGCAG 3 cut(s) 310, 1140, 3222
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 1827
BshFI GGCC 2 cut(s) 2412, 2550
BshTI ACCGGT 1 cut(s) 2133
BshVI ATCGAT 1 cut(s) 2455
BsiEI CGRYCG 1 cut(s) 1827
BsiHKAI GWGCWC 2 cut(s) 1085, 2641
BsiSI CCGG 2 cut(s) 1799, 2134
BsiWI CGTACG 1 cut(s) 2230
BslFI GGGAC 2 cut(s) 2554, 3443
BslI CCNNNNNNNGG 8 cut(s) 115, 1169, 1394, 2482, 2578, 3271, 3400, 3562
BsmAI GTCTC 5 cut(s) 395, 1500, 1566, 2258, 3021
BsmFI GGGAC 2 cut(s) 2554, 3443
BsmI GAATGC 2 cut(s) 2508, 2771
BsnI GGCC 2 cut(s) 2412, 2550
Bso31I GGTCTC 2 cut(s) 1566, 2258
Bsp119I TTCGAA 1 cut(s) 2500
Bsp1286I GDGCHC 3 cut(s) 855, 1085, 2641
Bsp1407I TGTACA 2 cut(s) 2442, 3015
Bsp19I CCATGG 1 cut(s) 1806
BspACI CCGC 7 cut(s) 64, 640, 1558, 2055, 2168, 3427, 3686
BspANI GGCC 2 cut(s) 2412, 2550
BspDI ATCGAT 1 cut(s) 2455
BspFNI CGCG 1 cut(s) 64
BspHI TCATGA 1 cut(s) 1939
BspLI GGNNCC 9 cut(s) 561, 1020, 1260, 2162, 2220, 2487, 2583, 3276, 3475
BspMAI CTGCAG 2 cut(s) 79, 1717
BspMI ACCTGC 1 cut(s) 3194
BspPI GGATC 6 cut(s) 554, 567, 1246, 1279, 2155, 2168
BspQI GCTCTTC 1 cut(s) 922
BspT104I TTCGAA 1 cut(s) 2500
BspTI CTTAAG 1 cut(s) 2776
BspTNI GGTCTC 2 cut(s) 1566, 2258
BsrBI CCGCTC 1 cut(s) 1558
BsrDI GCAATG 3 cut(s) 1164, 3219, 3240
BsrFI RCCGGY 1 cut(s) 2133
BsrGI TGTACA 2 cut(s) 2442, 3015
BsrI ACTGG 5 cut(s) 1288, 1525, 1733, 1843, 3502
BssAI RCCGGY 1 cut(s) 2133
BssECI CCNNGG 6 cut(s) 23, 576, 1331, 1806, 2571, 2588
BssT1I CCWWGG 5 cut(s) 23, 576, 1331, 1806, 2588
Bst2UI CCWGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
Bst6I CTCTTC 7 cut(s) 393, 738, 922, 1011, 2589, 2649, 3585
BstAFI CTTAAG 1 cut(s) 2776
BstAPI GCANNNNNTGC 3 cut(s) 800, 1198, 3548
BstAUI TGTACA 2 cut(s) 2442, 3015
BstBAI YACGTR 3 cut(s) 134, 1947, 2951
BstBI TTCGAA 1 cut(s) 2500
BstC8I GCNNGC 5 cut(s) 121, 1785, 2217, 2410, 3057
BstDSI CCRYGG 1 cut(s) 1806
BstENI CCTNNNNNAGG 3 cut(s) 3269, 3398, 3560
BstFNI CGCG 1 cut(s) 64
BstHHI GCGC 4 cut(s) 64, 1770, 2475, 2974
BstMAI GTCTC 5 cut(s) 395, 1500, 1566, 2258, 3021
BstMCI CGRYCG 1 cut(s) 1827
BstNI CCWGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
BstPAI GACNNNNGTC 1 cut(s) 3551
BstSCI CCNGG 8 cut(s) 108, 1029, 1393, 2083, 2422, 2571, 3277, 3305
BstSFI CTRYAG 4 cut(s) 75, 482, 1713, 1845
BstSNI TACGTA 1 cut(s) 1947
BstUI CGCG 1 cut(s) 64
BstV2I GAAGAC 5 cut(s) 1841, 2031, 2068, 3382, 3684
BstX2I RGATCY 3 cut(s) 559, 1238, 2160
BstYI RGATCY 3 cut(s) 559, 1238, 2160
Bsu15I ATCGAT 1 cut(s) 2455
BsuI GTATCC 2 cut(s) 3030, 3387
BsuRI GGCC 2 cut(s) 2412, 2550
BsuTUI ATCGAT 1 cut(s) 2455
BtgI CCRYGG 1 cut(s) 1806
BtgZI GCGATG 1 cut(s) 3129
BtrI CACGTC 1 cut(s) 1298
BtsI GCAGTG 2 cut(s) 322, 3050
BtsIMutI CAGTG 9 cut(s) 322, 679, 1306, 1740, 2843, 3015, 3050, 3228, 3624
BveI ACCTGC 1 cut(s) 3194
Cac8I GCNNGC 5 cut(s) 121, 1785, 2217, 2410, 3057
CaiI CAGNNNCTG 2 cut(s) 2153, 3230
CciI TCATGA 1 cut(s) 1939
CfoI GCGC 4 cut(s) 64, 1770, 2475, 2974
Cfr10I RCCGGY 1 cut(s) 2133
Cfr13I GGNCC 4 cut(s) 922, 1841, 2548, 2867
ClaI ATCGAT 1 cut(s) 2455
CseI GACGC 1 cut(s) 3134
CsiI ACCWGGT 2 cut(s) 1393, 3305
Csp6I GTAC 6 cut(s) 52, 1427, 1550, 2231, 2443, 3016
CspAI ACCGGT 1 cut(s) 2133
CspCI CAANNNNNGTGG 2 cut(s) 2239, 2274
CviQI GTAC 6 cut(s) 52, 1427, 1550, 2231, 2443, 3016
EaeI YGGCCR 1 cut(s) 2410
Eam1104I CTCTTC 7 cut(s) 393, 738, 922, 1011, 2589, 2649, 3585
EarI CTCTTC 7 cut(s) 393, 738, 922, 1011, 2589, 2649, 3585
Ecl136II GAGCTC 1 cut(s) 1083
Eco105I TACGTA 1 cut(s) 1947
Eco130I CCWWGG 5 cut(s) 23, 576, 1331, 1806, 2588
Eco24I GRGCYC 2 cut(s) 855, 1085
Eco31I GGTCTC 2 cut(s) 1566, 2258
Eco47I GGWCC 3 cut(s) 922, 1841, 2867
Eco53kI GAGCTC 1 cut(s) 1083
Eco57I CTGAAG 4 cut(s) 345, 719, 1932, 2592
EcoICRI GAGCTC 1 cut(s) 1083
EcoNI CCTNNNNNAGG 3 cut(s) 3269, 3398, 3560
EcoRI GAATTC 1 cut(s) 455
EcoRII CCWGG 8 cut(s) 108, 1029, 1393, 2083, 2422, 2571, 3277, 3305
EcoT14I CCWWGG 5 cut(s) 23, 576, 1331, 1806, 2588
EcoT22I ATGCAT 1 cut(s) 3145
EcoT38I GRGCYC 2 cut(s) 855, 1085
ErhI CCWWGG 5 cut(s) 23, 576, 1331, 1806, 2588
FalI AAGNNNNNCTT 6 cut(s) 919, 951, 1416, 1448, 3105, 3137
FaqI GGGAC 2 cut(s) 2554, 3443
FauI CCCGC 3 cut(s) 633, 2175, 3420
FauNDI CATATG 2 cut(s) 799, 3197
FbaI TGATCA 3 cut(s) 745, 2194, 3631
FblI GTMKAC 2 cut(s) 303, 1848
FriOI GRGCYC 2 cut(s) 855, 1085
FspI TGCGCA 1 cut(s) 2973
GlaI GCGC 4 cut(s) 63, 1769, 2474, 2973
GsaI CCCAGC 2 cut(s) 720, 1891
GsuI CTGGAG 2 cut(s) 1013, 2106
HaeIII GGCC 2 cut(s) 2412, 2550
HapII CCGG 2 cut(s) 1799, 2134
HgaI GACGC 1 cut(s) 3134
HhaI GCGC 4 cut(s) 64, 1770, 2475, 2974
Hin6I GCGC 4 cut(s) 62, 1768, 2473, 2972
HinP1I GCGC 4 cut(s) 62, 1768, 2473, 2972
HincII GTYRAC 5 cut(s) 812, 2661, 2698, 2850, 2890
HindII GTYRAC 5 cut(s) 812, 2661, 2698, 2850, 2890
HindIII AAGCTT 1 cut(s) 1460
HpaI GTTAAC 1 cut(s) 2661
HpaII CCGG 2 cut(s) 1799, 2134
HphI GGTGA 6 cut(s) 1193, 1383, 1583, 1904, 2742, 3168
Hpy99I CGWCG 1 cut(s) 2400
HpyCH4IV ACGT 7 cut(s) 133, 1297, 1946, 2229, 2395, 2700, 2950
HpySE526I ACGT 7 cut(s) 133, 1297, 1946, 2229, 2395, 2700, 2950
HspAI GCGC 4 cut(s) 62, 1768, 2473, 2972
Ksp22I TGATCA 3 cut(s) 745, 2194, 3631
KspAI GTTAAC 1 cut(s) 2661
LguI GCTCTTC 1 cut(s) 922
LmnI GCTCC 6 cut(s) 345, 478, 2144, 2224, 3280, 3479
MabI ACCWGGT 2 cut(s) 1393, 3305
MaeII ACGT 7 cut(s) 133, 1297, 1946, 2229, 2395, 2700, 2950
MaeIII GTNAC 8 cut(s) 727, 754, 1310, 1735, 2535, 2780, 2951, 3281
MbiI CCGCTC 1 cut(s) 1558
MfeI CAATTG 1 cut(s) 3180
MflI RGATCY 3 cut(s) 559, 1238, 2160
MhlI GDGCHC 3 cut(s) 855, 1085, 2641
MlyI GAGTC 4 cut(s) 273, 1374, 3063, 3425
MmeI TCCRAC 4 cut(s) 48, 2925, 3326, 3651
Mph1103I ATGCAT 1 cut(s) 3145
MroXI GAANNNNTTC 1 cut(s) 2943
MslI CAYNNNNRTG 2 cut(s) 288, 2043
MspA1I CMGCKG 1 cut(s) 770
MspCI CTTAAG 1 cut(s) 2776
MspI CCGG 2 cut(s) 1799, 2134
MspR9I CCNGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
MunI CAATTG 1 cut(s) 3180
Mva1269I GAATGC 2 cut(s) 2508, 2771
MvaI CCWGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
MvnI CGCG 1 cut(s) 64
NcoI CCATGG 1 cut(s) 1806
NdeI CATATG 2 cut(s) 799, 3197
NlaIV GGNNCC 9 cut(s) 561, 1020, 1260, 2162, 2220, 2487, 2583, 3276, 3475
NmuCI GTSAC 4 cut(s) 727, 1735, 2951, 3281
NsbI TGCGCA 1 cut(s) 2973
NsiI ATGCAT 1 cut(s) 3145
NspV TTCGAA 1 cut(s) 2500
PagI TCATGA 1 cut(s) 1939
PciSI GCTCTTC 1 cut(s) 922
PcsI WCGNNNNNNNCGW 2 cut(s) 2401, 2410
PctI GAATGC 2 cut(s) 2508, 2771
PdmI GAANNNNTTC 1 cut(s) 2943
Pfl23II CGTACG 1 cut(s) 2230
PflFI GACNNNGTC 1 cut(s) 1364
PflMI CCANNNNNTGG 1 cut(s) 2578
PinAI ACCGGT 1 cut(s) 2133
PleI GAGTC 4 cut(s) 273, 1373, 3063, 3425
PpsI GAGTC 4 cut(s) 273, 1373, 3063, 3425
Ppu21I YACGTR 3 cut(s) 134, 1947, 2951
PshAI GACNNNNGTC 1 cut(s) 3551
PshBI ATTAAT 1 cut(s) 1923
PsiI TTATAA 1 cut(s) 2331
Psp124BI GAGCTC 1 cut(s) 1085
Psp6I CCWGG 8 cut(s) 108, 1029, 1393, 2083, 2422, 2571, 3277, 3305
PspFI CCCAGC 2 cut(s) 716, 1887
PspGI CCWGG 8 cut(s) 108, 1029, 1393, 2083, 2422, 2571, 3277, 3305
PspLI CGTACG 1 cut(s) 2230
PspN4I GGNNCC 9 cut(s) 561, 1020, 1260, 2162, 2220, 2487, 2583, 3276, 3475
PspPI GGNCC 4 cut(s) 922, 1841, 2548, 2867
PstI CTGCAG 2 cut(s) 79, 1717
PstNI CAGNNNCTG 2 cut(s) 2153, 3230
PsuI RGATCY 3 cut(s) 559, 1238, 2160
PsyI GACNNNGTC 1 cut(s) 1364
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 6 cut(s) 53, 1428, 1551, 2232, 2444, 3017
RsaNI GTAC 6 cut(s) 52, 1427, 1550, 2231, 2443, 3016
RseI CAYNNNNRTG 2 cut(s) 288, 2043
SacI GAGCTC 1 cut(s) 1085
SapI GCTCTTC 1 cut(s) 922
Sau96I GGNCC 4 cut(s) 922, 1841, 2548, 2867
SchI GAGTC 4 cut(s) 273, 1374, 3063, 3425
ScrFI CCNGG 8 cut(s) 110, 1031, 1395, 2085, 2424, 2573, 3279, 3307
SduI GDGCHC 3 cut(s) 855, 1085, 2641
SexAI ACCWGGT 2 cut(s) 1393, 3305
SfcI CTRYAG 4 cut(s) 75, 482, 1713, 1845
SfuI TTCGAA 1 cut(s) 2500
SinI GGWCC 3 cut(s) 922, 1841, 2867
SmiMI CAYNNNNRTG 2 cut(s) 288, 2043
SmlI CTYRAG 3 cut(s) 140, 1751, 2776
SmoI CTYRAG 3 cut(s) 140, 1751, 2776
SnaBI TACGTA 1 cut(s) 1947
SpeI ACTAGT 1 cut(s) 2447
SsiI CCGC 7 cut(s) 64, 640, 1558, 2055, 2168, 3427, 3686
SspI AATATT 2 cut(s) 2257, 2736
SstI GAGCTC 1 cut(s) 1085
StyD4I CCNGG 8 cut(s) 108, 1029, 1393, 2083, 2422, 2571, 3277, 3305
StyI CCWWGG 5 cut(s) 23, 576, 1331, 1806, 2588
TaiI ACGT 7 cut(s) 136, 1300, 1949, 2232, 2398, 2703, 2953
TaqI TCGA 8 cut(s) 209, 1823, 1972, 2351, 2398, 2455, 2500, 3396
TaqII GACCGA 2 cut(s) 939, 1841
TatI WGTACW 3 cut(s) 1549, 2442, 3015
TauI GCSGC 2 cut(s) 2058, 3688
TscAI CASTG 9 cut(s) 329, 679, 1313, 1740, 2850, 3015, 3057, 3235, 3631
TseFI GTSAC 4 cut(s) 727, 1735, 2951, 3281
Tsp45I GTSAC 4 cut(s) 727, 1735, 2951, 3281
TspGWI ACGGA 2 cut(s) 2663, 3560
TspRI CASTG 9 cut(s) 329, 679, 1313, 1740, 2850, 3015, 3057, 3235, 3631
Tth111I GACNNNGTC 1 cut(s) 1364
Van91I CCANNNNNTGG 1 cut(s) 2578
Vha464I CTTAAG 1 cut(s) 2776
VpaK11BI GGWCC 3 cut(s) 922, 1841, 2867
VspI ATTAAT 1 cut(s) 1923
XagI CCTNNNNNAGG 3 cut(s) 3269, 3398, 3560
XapI RAATTY 9 cut(s) 191, 455, 523, 1127, 1496, 1978, 2097, 2427, 3368
XcmI CCANNNNNNNNNTGG 2 cut(s) 1295, 3494
XmiI GTMKAC 2 cut(s) 303, 1848
XmnI GAANNNNTTC 1 cut(s) 2943
Zsp2I ATGCAT 1 cut(s) 3145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.