Rh7CG258000

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0007888
Physical Location & Seq
Reverse (-)
25117575 .. 25123602
6028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG258000.1

Sequence Viewer

Length: 4440 bp
ATGGCTCCAAAGAAGAAGCAGCCGCAGCAGCAGCCCAAGAAGCCCAGCAGTAAACCCCAACCGGCCTCGTCTGGCCAGAAGCCCCAGCCCAACACCGCCAAACCCCAAACGACGTCCTCCGGCCCCAGGCTCCAGATTTCCGCCGAGAACGAGAACCGCCTCCGCCGCCTCCTCCTCAACTCCGGCCGTTCCGCCGCGGTCCCCGTCGACGAGTCGCTCTCCAAGGCTCAGAAGGCCAAGAAGCTCAGGGCCGTGTACGAGCAGCTCTCCTGCGAAGGCTTCACCAATGATCAGATTGAACTCGCTCTCTCCGCCGTCAAGGAGGGGGCTACATATGAGGCTGCTCTGGACTGGTTGTGCTTAAATGTGCCTGGGAATGAGCTCCCACTCAAGTTTTCGAGTGGTGTTTCGATGGCTGCAAATGAAGGTGGTTCTGTTGGTGTTGTACTGACTTCTCGGGACGACTGGACTCCGTCGGTGGATACGTCTGCTAAGATTGATGAGGATACGCCGGGGATTGCTATTAGGACCAAGGGGCAGTGGAGTGATAAAACACTTGATTCATGTCAACCGTCTCAGGCAGATTGGATCAAACGATATGTTGAGCAGCAAGAAGAGGATGAATCGAGTACATGGGAAGATGATGTAGATGATGAGGGTATGGGCGCTAAGGTCAGTAAACCAAGGTCTTATGATGTTATAGCCAAAGAGTACCACGCTGCGCGGTTGGAAGCTGCAGAAGCCAAGCAACAAAAGGACAAGAAAAACCAGGAACGGGCAGGCAAAATGATACGTGATCTCAAGCAAGAGTTGGCTGCTCTAGGCTTATCGGACGACATCTTGGCATTGGAATTTGAACAGGAACAGTCGATAGAACGAGCTTATTCTGCTTTTGAGGACACAGATGCTAGTTCAACGCCTTACAAGCAGTCTGAAGGACTCCATGCTGATGAATTAAAAGTAGACGGTAATGATATGGAACACTGCAGCTCTGTTCAGCTCCCAATCAACTCTACTCCTTCAGATTTACCTGTTCAGGAAAAAATTGTTGCAGAAGAAGAGACAACTGATATGGAAATTGGTAATTTTTTCTTGGAAGATGCACCATCTAACGAATTCCTAACTCCTACTATTTTGGAACTACAGAAGAAAGAAAAGTTGAGAGAAATGCTCAGTGAGAAAAATTTGGAGAAATTAGATGGTATTTGGAAGAAGGGGGATCCTCAAAAGATTCCCAAGGCTATTTTTCAGCAATTATGTCAAAAATCAGGATGGGAAGCCCCAAAGTTTCATAAAGTGCGGGGAAAGGATAATAGTTTCTCTTATACTATCAGTGTTTTGCGTAAAGCTAGTGGAAGGGGTAAGAGCAGAAAGGCTGGGGGTTTGGTCACTCTTCAGCTTCCTGATCACGATGTAACTTTTGATTCAGCTGAGGATGCACAGAATAGAGTGGCAGCATATGCTCTTTGTCAACTGTTTACTGATCTCCCAATCCATCTTGTAATTACTGAGCCCTATGCTTCTCTAGTTGTTCAATGGAAGGAAGGGGAAACAATAACCAATGTAGAAGATAGTGTTAAGGACCGAAGAGCTACTTTTGTGGATTCACTATTGAAAGCTGATGGTTCTACTTCAACTTCTGCTGCTAATGTTGTATATGATTCTGATTCTCTTCCTAAAGTGGTTCCACAACTCCAGGTTCAAGAACCTAGAAATGCAGAACTAAATCCTCGCAAAGATGCAGAGAGCTCTTATTTGAAACAAGAACTGGAGAATAAACAGAAAAAGCAGAAATTCAAGGAAATGTTGAGAGCTAGAGCTGCACTCCCCATTGCTGGTTTGAAGGGTGATATGTTGCAACTGTTGCAGGATAATAATGTTCTAGTAGTTTGTGGGGAAACGGGATCTGGAAAAACAACTCAGGTTCCACAATTTATATTGGATGACATGATCCAGTCAGGACGTGGTGGACACTGTAACATTATATGCACACAACCAAGGAGAATAGCAGCTATTTCTGTGGCTGACAGAGTCGCTGATGAGCGTTGCGAACCTTCACCCGGTTCAAATGGTTCTTTGGTTGGTTATCAAGTACGCCTTGATAATGCAAGTAATGAAAAGACTAAGCTTCTTTTTTGCACGACAGGTATTCTTCTGAGAAAATTCGTGGGAGACAGAAACTTGACTGGAATTACTCATGTCATAGTTGATGAAGTACATGAGCGGTCTCTTTTGGGTGATTTCCTGCTCATAGTTTTGAAGAATCTGATTGAGAAGCAATCAGCTCTTAACACACCAAAACTGAAAGTTATTCTAATGTCTGCAACTGTTGATTCCAATTTGTTTTCAAATTACTTTGGTGGTTGTCCTGTGATTACTGCAGAAGGTAGAACACATCCAGTGACGACCTATTTTCTTGAGGATATTTATGAGCGCATTGATTATCGGCTTGCATCAGATTCTCCGGCTTCCATGGTCTATGGAACTTCGACCGAAGGGAAGACTGGTCCTGTAGACAACAGCAGAGGAAAGAAGAATCTTGTTTTATCTGGCTGGGGTGATGATTCTCTACTCTCTGAAGAATATATTAATCCAAATTATGTTCATGATACGTATCAATCTTACAGAGAGCAAACTCGACAAAATTTGAAAAGATTGAATGAAGATGTCATTGATTATGATCTTCTTGAAGACTTGGTGTGCCATGTTGATGAAACTTGCGGCGAAGGTGCTATTCTTGTCTTCTTGCCTGGAGTATCTGAAATTTACACCTTAGTTGATAAACTAGCTGCTTCTTACCGGTTTGGTGGAGCAGCTTCTGATTGGATCCTCCCGCTACATTCATCTGTTGCATCAGTTGATCAAAAGAAGGTGTTTTTGCAGGCTCCTGAGAACATACGAAAGATTATTGTAGCCACTAATATTGCTGAGACCAGCATAACAATAGATGATGTGGTCTATGTTATTGACTGTGGAAAGCATAAGGAGAATCGTTATAATCCTCAGAAGAAATTGTCGAGCATGGTTGAGGATTGGATTTCTAAAGCAAATGCAAGGCAACGTCGAGGACGAGCTGGCCGCGTGAAACCTGGAATTTGCTTTTGCCTGTACACTAGTTATCGATTTGAAAAGCTGATGCGCCCCTTTCAGGTTCCAGAGATGCTTCGAATGCCATTAGTAGAGTTGTGTTTACAAATTAAGTTACTTTCTCTGGGCCACATAAAACCATTTTTGTCCCAGGCTTTGGAACCTCCAAGGGAAGAGGCTATGACTTCAGCAATTAAGATATTGTATGAGGTTGGTGCTCTTGAAACGGATGAAGAGTTAACGCCTCTTGGGCATCATTTGGCGAAACTTCCTGTTGACGTATTGATTGGGAAGATGATGATATATGGTGGAATATTTGGTTGCTTATCACCCATTCTATCAATTTCAGCATTCTTAAGTTACAAATCTCCATTTGTCCATCCAAAAGATGAGAAGGAAAATGCTAAAAGAGCAAAATTGGCACTGTTGACTGACAAGCTAGATGGTCCAAGTGAATCAAATGATGTTGACAAGCAATCCGACCATCTCATAATGATAACTGCATACAAGAAATGGGAAAAGATTCTACGTGACAAAGGGGTTAGAGCTGCGCAGCAGTTTTGCAATTCATATTATTTGAGCAGTTCGGTGATGTATATGATAAGAGACATGAGGATACAGTTTGGAACACTGCTTGCTGATATTGGACTCATTGATCTTCCAAAGAAATATCAGGTTGATGGAAGAAAGAAAGAGAATCTTGACGCTTGGTTTTCGGATGCATTGCAACCCTTCAATATGTATTCAAATCATTCACCAATTGTAAAAGCTATCATATGTGCAGGTTTATACCCTAACATTGCAGCCACTGAGAAAGGCATTGCTGGAACAGTCCTCAATAACCTTAAACAGGCTCCTGGTCACGCAACTAGCCATTGTCCAACCTGGTATGATGGAAGAAGAAAAGTCAATGTACACCCTTCTTCTATCAACCATAATGTAAATGAATTTCGGTATCCTTTCCTTGTCTTCCTCGAAAAAGTTGAAACCAACAAAGTATTTTTGCGGGACTCTACAATCATTTCTCCCAACTCTATTTTGCTGTTTGGTGGCTCCATTAACATCCAGCATCAGACTGGACTTGTAATTGTTGATGGATGGTTAAAACTGACAGCACCAGCACAGACTGCTGTCCTGTTCAAGGAACTCCGTTTAACTCTTCACTCTGTTCTGAAAGAATTGATAAGAAAGCCTGAGGTAACATTCTTTTTGCTTATAAGTTTCGATACTTGGATTATTTATTTCTTGTTTTGGCGTGACGTTTCATTCATCTGTAACATTAGAATTTTCTTATGTCTAATCAGTTCTGTCCCATACTGGATTTCAATGTCCCAGATGATCCAAAGAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016282 GO:0032991 GO:0043021 GO:0043024 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0070993 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1479

Amino Acids

165.34

Weight (kDa)

6.15

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_DHX29 PF24899 81 - 133 1.7e-17 ATP-dependent RNA helicase DHX29, UBA domain
DSRM_DHX29 PF24385 410 - 515 7.4e-06 ATP-dependent RNA helicase DHX29, DSRM-like
DEAD PF00270 621 - 780 2.5e-10 DEAD/DEAH box helicase
Helicase_C PF00271 905 - 1028 1.5e-09 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1093 - 1121 2.8e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1125 - 1193 3.2e-10 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1284 - 1382 9.5e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1389 - 1429 7.5e-12 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2997, 4304
AatII GACGTC 1 cut(s) 116
Acc16I TGCGCA 1 cut(s) 3639
Acc36I ACCTGC 1 cut(s) 3860
AccB7I CCANNNNNTGG 1 cut(s) 3244
AccBSI CCGCTC 1 cut(s) 2224
AccI GTMKAC 3 cut(s) 207, 963, 2514
AccII CGCG 3 cut(s) 197, 724, 3081
AclWI GGATC 8 cut(s) 596, 1214, 1227, 1912, 1945, 2821, 2834, 4420
AcoI YGGCCR 3 cut(s) 73, 184, 3076
AcuI CTGAAG 5 cut(s) 954, 1005, 1379, 2598, 3258
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 8 cut(s) 257, 447, 631, 713, 2094, 2217, 3110, 4002
AfiI CCNNNNNNNGG 8 cut(s) 126, 775, 1835, 2060, 3148, 3244, 3937, 4228
AflII CTTAAG 1 cut(s) 3442
AgeI ACCGGT 1 cut(s) 2799
AhlI ACTAGT 1 cut(s) 3113
AjiI CACGTC 1 cut(s) 1964
AjnI CCWGG 9 cut(s) 125, 370, 768, 1695, 2749, 3088, 3237, 3943, 3971
Alw21I GWGCWC 3 cut(s) 384, 1751, 3307
Alw26I GTCTC 6 cut(s) 579, 1055, 2166, 2232, 2924, 3687
AlwI GGATC 8 cut(s) 596, 1214, 1227, 1912, 1945, 2821, 2834, 4420
AlwNI CAGNNNCTG 2 cut(s) 2819, 3896
Ama87I CYCGRG 1 cut(s) 456
AoxI GGCC 8 cut(s) 63, 73, 121, 184, 234, 249, 3076, 3214
AseI ATTAAT 1 cut(s) 2589
AsiGI ACCGGT 1 cut(s) 2799
Asp700I GAANNNNTTC 1 cut(s) 3609
AspLEI GCGC 5 cut(s) 668, 724, 2436, 3141, 3640
AspS9I GGNCC 8 cut(s) 122, 199, 249, 528, 1582, 2507, 3214, 3533
AsuC2I CCSGG 2 cut(s) 513, 2061
AsuHPI GGTGA 8 cut(s) 274, 1859, 2049, 2249, 2570, 3408, 3688, 3834
AsuII TTCGAA 1 cut(s) 3166
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 5 cut(s) 199, 528, 1582, 2507, 3533
AxyI CCTNAGG 1 cut(s) 4281
BalI TGGCCA 1 cut(s) 75
BamHI GGATCC 2 cut(s) 1219, 2826
BanII GRGCYC 3 cut(s) 384, 1515, 1751
BbsI GAAGAC 4 cut(s) 2507, 2697, 2734, 4048
Bbv12I GWGCWC 3 cut(s) 384, 1751, 3307
BbvCI CCTCAGC 1 cut(s) 1431
BceAI ACGGC 3 cut(s) 171, 236, 299
BciT130I CCWGG 9 cut(s) 127, 372, 770, 1697, 2751, 3090, 3239, 3945, 3973
BciVI GTATCC 4 cut(s) 475, 499, 3696, 4053
BclI TGATCA 3 cut(s) 289, 1405, 2860
BcnI CCSGG 2 cut(s) 513, 2061
BcoDI GTCTC 6 cut(s) 579, 1055, 2166, 2232, 2924, 3687
BcuI ACTAGT 1 cut(s) 3113
BfmI CTRYAG 5 cut(s) 735, 985, 1142, 2379, 2511
BfoI RGCGCY 1 cut(s) 669
BfrI CTTAAG 1 cut(s) 3442
BfuAI ACCTGC 1 cut(s) 3860
BfuI GTATCC 4 cut(s) 475, 499, 3696, 4053
BglI GCCNNNNNGGC 1 cut(s) 3337
Bme18I GGWCC 5 cut(s) 199, 528, 1582, 2507, 3533
BmeT110I CYCGRG 1 cut(s) 456
BmgBI CACGTC 1 cut(s) 1964
BmgT120I GGNCC 8 cut(s) 122, 199, 249, 528, 1582, 2507, 3214, 3533
BoxI GACNNNNGTC 1 cut(s) 4217
BpiI GAAGAC 4 cut(s) 2507, 2697, 2734, 4048
BplI GAGNNNNNCTC 8 cut(s) 203, 235, 251, 283, 1155, 1187, 1809, 1841
BpmI CTGGAG 4 cut(s) 116, 1679, 1790, 2772
Bpu10I CCTNAGC 3 cut(s) 245, 669, 1431
Bpu14I TTCGAA 1 cut(s) 3166
BpuEI CTTGAG 3 cut(s) 374, 785, 2438
BpuMI CCSGG 2 cut(s) 513, 2061
Bsa29I ATCGAT 1 cut(s) 3121
BsaAI YACGTR 3 cut(s) 794, 2613, 3617
BsaBI GATNNNNATC 2 cut(s) 2613, 2679
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 2 cut(s) 2232, 2924
BsaWI WCCGGW 1 cut(s) 2799
Bsc4I CCNNNNNNNGG 8 cut(s) 126, 775, 1835, 2060, 3148, 3244, 3937, 4228
Bse118I RCCGGY 2 cut(s) 61, 2799
Bse1I ACTGG 9 cut(s) 356, 470, 1773, 1954, 2191, 2399, 2509, 4168, 4409
Bse21I CCTNAGG 1 cut(s) 4281
Bse3DI GCAATG 4 cut(s) 1830, 3809, 3885, 3906
Bse8I GATNNNNATC 2 cut(s) 2613, 2679
BseBI CCWGG 9 cut(s) 127, 372, 770, 1697, 2751, 3090, 3239, 3945, 3973
BseCI ATCGAT 1 cut(s) 3121
BseJI GATNNNNATC 2 cut(s) 2613, 2679
BseLI CCNNNNNNNGG 8 cut(s) 126, 775, 1835, 2060, 3148, 3244, 3937, 4228
BseMI GCAATG 4 cut(s) 1830, 3809, 3885, 3906
BseNI ACTGG 9 cut(s) 356, 470, 1773, 1954, 2191, 2399, 2509, 4168, 4409
BseRI GAGGAG 2 cut(s) 161, 164
BseX3I CGGCCG 1 cut(s) 184
BseYI CCCAGC 4 cut(s) 44, 84, 1376, 2553
BsgI GTGCAG 2 cut(s) 1806, 3888
Bsh1236I CGCG 3 cut(s) 197, 724, 3081
Bsh1285I CGRYCG 2 cut(s) 187, 2493
BshFI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
BshTI ACCGGT 1 cut(s) 2799
BshVI ATCGAT 1 cut(s) 3121
BsiEI CGRYCG 2 cut(s) 187, 2493
BsiHKAI GWGCWC 3 cut(s) 384, 1751, 3307
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 7 cut(s) 62, 120, 183, 512, 2061, 2465, 2800
BslFI GGGAC 6 cut(s) 185, 473, 3220, 4109, 4382, 4402
BslI CCNNNNNNNGG 8 cut(s) 126, 775, 1835, 2060, 3148, 3244, 3937, 4228
BsmAI GTCTC 6 cut(s) 579, 1055, 2166, 2232, 2924, 3687
BsmBI CGTCTC 1 cut(s) 579
BsmFI GGGAC 6 cut(s) 185, 473, 3220, 4109, 4382, 4402
BsmI GAATGC 2 cut(s) 3174, 3437
BsnI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
Bso31I GGTCTC 2 cut(s) 2232, 2924
BsoBI CYCGRG 1 cut(s) 456
Bsp119I TTCGAA 1 cut(s) 3166
Bsp1286I GDGCHC 4 cut(s) 384, 1515, 1751, 3307
Bsp1407I TGTACA 2 cut(s) 3108, 4000
Bsp19I CCATGG 1 cut(s) 2472
BspANI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
BspDI ATCGAT 1 cut(s) 3121
BspFNI CGCG 3 cut(s) 197, 724, 3081
BspHI TCATGA 1 cut(s) 2605
BspMAI CTGCAG 3 cut(s) 739, 989, 2383
BspMI ACCTGC 1 cut(s) 3860
BspPI GGATC 8 cut(s) 596, 1214, 1227, 1912, 1945, 2821, 2834, 4420
BspQI GCTCTTC 1 cut(s) 1582
BspT104I TTCGAA 1 cut(s) 3166
BspTI CTTAAG 1 cut(s) 3442
BspTNI GGTCTC 2 cut(s) 2232, 2924
BsrBI CCGCTC 1 cut(s) 2224
BsrDI GCAATG 4 cut(s) 1830, 3809, 3885, 3906
BsrFI RCCGGY 2 cut(s) 61, 2799
BsrGI TGTACA 2 cut(s) 3108, 4000
BsrI ACTGG 9 cut(s) 356, 470, 1773, 1954, 2191, 2399, 2509, 4168, 4409
BssAI RCCGGY 2 cut(s) 61, 2799
BssNI GRCGYC 1 cut(s) 113
BssT1I CCWWGG 7 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254
Bst2UI CCWGG 9 cut(s) 127, 372, 770, 1697, 2751, 3090, 3239, 3945, 3973
Bst6I CTCTTC 8 cut(s) 609, 1053, 1398, 1582, 1677, 3255, 3315, 4251
BstACI GRCGYC 1 cut(s) 113
BstAFI CTTAAG 1 cut(s) 3442
BstAPI GCANNNNNTGC 3 cut(s) 1460, 1864, 4214
BstAUI TGTACA 2 cut(s) 3108, 4000
BstBAI YACGTR 3 cut(s) 794, 2613, 3617
BstBI TTCGAA 1 cut(s) 3166
BstC8I GCNNGC 5 cut(s) 781, 2451, 2883, 3076, 3723
BstDSI CCRYGG 2 cut(s) 195, 2472
BstENI CCTNNNNNAGG 2 cut(s) 3935, 4226
BstFNI CGCG 3 cut(s) 197, 724, 3081
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 5 cut(s) 668, 724, 2436, 3141, 3640
BstMAI GTCTC 6 cut(s) 579, 1055, 2166, 2232, 2924, 3687
BstMCI CGRYCG 2 cut(s) 187, 2493
BstNI CCWGG 9 cut(s) 127, 372, 770, 1697, 2751, 3090, 3239, 3945, 3973
BstPAI GACNNNNGTC 1 cut(s) 4217
BstSFI CTRYAG 5 cut(s) 735, 985, 1142, 2379, 2511
BstSNI TACGTA 1 cut(s) 2613
BstUI CGCG 3 cut(s) 197, 724, 3081
BstV2I GAAGAC 4 cut(s) 2507, 2697, 2734, 4048
BstX2I RGATCY 3 cut(s) 1219, 1904, 2826
BstYI RGATCY 3 cut(s) 1219, 1904, 2826
BstZI CGGCCG 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 3121
Bsu36I CCTNAGG 1 cut(s) 4281
BsuI GTATCC 4 cut(s) 475, 499, 3696, 4053
BsuRI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
BsuTUI ATCGAT 1 cut(s) 3121
BtgI CCRYGG 2 cut(s) 195, 2472
BtrI CACGTC 1 cut(s) 1964
BtsI GCAGTG 3 cut(s) 545, 982, 3716
BtsIMutI CAGTG 9 cut(s) 545, 982, 1180, 1339, 1972, 2406, 3509, 3716, 3894
BveI ACCTGC 1 cut(s) 3860
Cac8I GCNNGC 5 cut(s) 781, 2451, 2883, 3076, 3723
CaiI CAGNNNCTG 2 cut(s) 2819, 3896
CciI TCATGA 1 cut(s) 2605
CfoI GCGC 5 cut(s) 668, 724, 2436, 3141, 3640
Cfr10I RCCGGY 2 cut(s) 61, 2799
Cfr13I GGNCC 8 cut(s) 122, 199, 249, 528, 1582, 2507, 3214, 3533
Cfr42I CCGCGG 1 cut(s) 198
ClaI ATCGAT 1 cut(s) 3121
CseI GACGC 1 cut(s) 3800
CsiI ACCWGGT 1 cut(s) 3971
Csp6I GTAC 8 cut(s) 256, 446, 630, 712, 2093, 2216, 3109, 4001
CspAI ACCGGT 1 cut(s) 2799
CspCI CAANNNNNGTGG 2 cut(s) 2905, 2940
CviQI GTAC 8 cut(s) 256, 446, 630, 712, 2093, 2216, 3109, 4001
EaeI YGGCCR 3 cut(s) 73, 184, 3076
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 8 cut(s) 609, 1053, 1398, 1582, 1677, 3255, 3315, 4251
EarI CTCTTC 8 cut(s) 609, 1053, 1398, 1582, 1677, 3255, 3315, 4251
EciI GGCGGA 4 cut(s) 130, 152, 181, 301
Ecl136II GAGCTC 2 cut(s) 382, 1749
EclXI CGGCCG 1 cut(s) 184
Eco105I TACGTA 1 cut(s) 2613
Eco130I CCWWGG 7 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254
Eco24I GRGCYC 3 cut(s) 384, 1515, 1751
Eco31I GGTCTC 2 cut(s) 2232, 2924
Eco47I GGWCC 5 cut(s) 199, 528, 1582, 2507, 3533
Eco52I CGGCCG 1 cut(s) 184
Eco53kI GAGCTC 2 cut(s) 382, 1749
Eco57I CTGAAG 5 cut(s) 954, 1005, 1379, 2598, 3258
Eco81I CCTNAGG 1 cut(s) 4281
Eco88I CYCGRG 1 cut(s) 456
EcoICRI GAGCTC 2 cut(s) 382, 1749
EcoNI CCTNNNNNAGG 2 cut(s) 3935, 4226
EcoRI GAATTC 1 cut(s) 1115
EcoRII CCWGG 9 cut(s) 125, 370, 768, 1695, 2749, 3088, 3237, 3943, 3971
EcoT14I CCWWGG 7 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254
EcoT22I ATGCAT 1 cut(s) 3811
EcoT38I GRGCYC 3 cut(s) 384, 1515, 1751
ErhI CCWWGG 7 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254
Esp3I CGTCTC 1 cut(s) 579
FalI AAGNNNNNCTT 6 cut(s) 1579, 1611, 2082, 2114, 3771, 3803
FaqI GGGAC 6 cut(s) 185, 473, 3220, 4109, 4382, 4402
FauI CCCGC 3 cut(s) 1293, 2841, 4086
FauNDI CATATG 3 cut(s) 334, 1459, 3863
FbaI TGATCA 3 cut(s) 289, 1405, 2860
FblI GTMKAC 3 cut(s) 207, 963, 2514
FriOI GRGCYC 3 cut(s) 384, 1515, 1751
FspI TGCGCA 1 cut(s) 3639
GlaI GCGC 5 cut(s) 667, 723, 2435, 3140, 3639
GsaI CCCAGC 4 cut(s) 48, 88, 1380, 2557
GsuI CTGGAG 4 cut(s) 116, 1679, 1790, 2772
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
HapII CCGG 7 cut(s) 62, 120, 183, 512, 2061, 2465, 2800
HgaI GACGC 1 cut(s) 3800
HhaI GCGC 5 cut(s) 668, 724, 2436, 3141, 3640
Hin1I GRCGYC 1 cut(s) 113
Hin6I GCGC 5 cut(s) 666, 722, 2434, 3139, 3638
HinP1I GCGC 5 cut(s) 666, 722, 2434, 3139, 3638
HincII GTYRAC 7 cut(s) 208, 569, 1472, 3327, 3364, 3516, 3556
HindII GTYRAC 7 cut(s) 208, 569, 1472, 3327, 3364, 3516, 3556
HindIII AAGCTT 1 cut(s) 2126
HpaI GTTAAC 1 cut(s) 3327
HpaII CCGG 7 cut(s) 62, 120, 183, 512, 2061, 2465, 2800
HphI GGTGA 8 cut(s) 274, 1859, 2049, 2249, 2570, 3408, 3688, 3834
Hpy99I CGWCG 5 cut(s) 115, 209, 212, 478, 3066
HpyCH4IV ACGT 9 cut(s) 113, 485, 793, 1963, 2612, 3061, 3366, 3616, 4347
HpySE526I ACGT 9 cut(s) 113, 485, 793, 1963, 2612, 3061, 3366, 3616, 4347
Hsp92I GRCGYC 1 cut(s) 113
HspAI GCGC 5 cut(s) 666, 722, 2434, 3139, 3638
Ksp22I TGATCA 3 cut(s) 289, 1405, 2860
KspAI GTTAAC 1 cut(s) 3327
KspI CCGCGG 1 cut(s) 198
LguI GCTCTTC 1 cut(s) 1582
LmnI GCTCC 8 cut(s) 10, 135, 387, 1005, 2810, 2890, 3946, 4145
MabI ACCWGGT 1 cut(s) 3971
MaeII ACGT 9 cut(s) 113, 485, 793, 1963, 2612, 3061, 3366, 3616, 4347
MbiI CCGCTC 1 cut(s) 2224
MfeI CAATTG 1 cut(s) 3846
MflI RGATCY 3 cut(s) 1219, 1904, 2826
MhlI GDGCHC 4 cut(s) 384, 1515, 1751, 3307
MlsI TGGCCA 1 cut(s) 75
MluNI TGGCCA 1 cut(s) 75
MlyI GAGTC 6 cut(s) 221, 463, 933, 2040, 3729, 4091
MmeI TCCRAC 3 cut(s) 708, 3591, 3992
Mox20I TGGCCA 1 cut(s) 75
Mph1103I ATGCAT 1 cut(s) 3811
MroXI GAANNNNTTC 1 cut(s) 3609
MscI TGGCCA 1 cut(s) 75
MslI CAYNNNNRTG 2 cut(s) 948, 2709
Msp20I TGGCCA 1 cut(s) 75
MspA1I CMGCKG 2 cut(s) 197, 1430
MspCI CTTAAG 1 cut(s) 3442
MspI CCGG 7 cut(s) 62, 120, 183, 512, 2061, 2465, 2800
MunI CAATTG 1 cut(s) 3846
Mva1269I GAATGC 2 cut(s) 3174, 3437
MvaI CCWGG 9 cut(s) 127, 372, 770, 1697, 2751, 3090, 3239, 3945, 3973
MvnI CGCG 3 cut(s) 197, 724, 3081
NciI CCSGG 2 cut(s) 513, 2061
NcoI CCATGG 1 cut(s) 2472
NdeI CATATG 3 cut(s) 334, 1459, 3863
NmeAIII GCCGAG 1 cut(s) 169
NmuCI GTSAC 5 cut(s) 1387, 2401, 3617, 3947, 4343
NsbI TGCGCA 1 cut(s) 3639
NsiI ATGCAT 1 cut(s) 3811
NspV TTCGAA 1 cut(s) 3166
PagI TCATGA 1 cut(s) 2605
PciSI GCTCTTC 1 cut(s) 1582
PcsI WCGNNNNNNNCGW 2 cut(s) 482, 3067
PctI GAATGC 2 cut(s) 3174, 3437
PdmI GAANNNNTTC 1 cut(s) 3609
PflFI GACNNNGTC 2 cut(s) 472, 2030
PflMI CCANNNNNTGG 1 cut(s) 3244
PinAI ACCGGT 1 cut(s) 2799
PleI GAGTC 6 cut(s) 220, 463, 933, 2039, 3729, 4091
PpsI GAGTC 6 cut(s) 220, 463, 933, 2039, 3729, 4091
Ppu21I YACGTR 3 cut(s) 794, 2613, 3617
PshAI GACNNNNGTC 1 cut(s) 4217
PshBI ATTAAT 1 cut(s) 2589
PsiI TTATAA 2 cut(s) 2997, 4304
Psp124BI GAGCTC 2 cut(s) 384, 1751
Psp6I CCWGG 9 cut(s) 125, 370, 768, 1695, 2749, 3088, 3237, 3943, 3971
PspFI CCCAGC 4 cut(s) 44, 84, 1376, 2553
PspGI CCWGG 9 cut(s) 125, 370, 768, 1695, 2749, 3088, 3237, 3943, 3971
PspPI GGNCC 8 cut(s) 122, 199, 249, 528, 1582, 2507, 3214, 3533
PstI CTGCAG 3 cut(s) 739, 989, 2383
PstNI CAGNNNCTG 2 cut(s) 2819, 3896
PsuI RGATCY 3 cut(s) 1219, 1904, 2826
PsyI GACNNNGTC 2 cut(s) 472, 2030
PvuII CAGCTG 1 cut(s) 1430
RsaI GTAC 8 cut(s) 257, 447, 631, 713, 2094, 2217, 3110, 4002
RsaNI GTAC 8 cut(s) 256, 446, 630, 712, 2093, 2216, 3109, 4001
RseI CAYNNNNRTG 2 cut(s) 948, 2709
SacI GAGCTC 2 cut(s) 384, 1751
SacII CCGCGG 1 cut(s) 198
SalI GTCGAC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 1582
Sau96I GGNCC 8 cut(s) 122, 199, 249, 528, 1582, 2507, 3214, 3533
SchI GAGTC 6 cut(s) 221, 463, 933, 2040, 3729, 4091
SduI GDGCHC 4 cut(s) 384, 1515, 1751, 3307
SexAI ACCWGGT 1 cut(s) 3971
SfcI CTRYAG 5 cut(s) 735, 985, 1142, 2379, 2511
Sfr303I CCGCGG 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 3166
SgrBI CCGCGG 1 cut(s) 198
SgrDI CGTCGACG 1 cut(s) 206
SinI GGWCC 5 cut(s) 199, 528, 1582, 2507, 3533
SmiMI CAYNNNNRTG 2 cut(s) 948, 2709
SmlI CTYRAG 4 cut(s) 389, 800, 2417, 3442
SmoI CTYRAG 4 cut(s) 389, 800, 2417, 3442
SnaBI TACGTA 1 cut(s) 2613
SpeI ACTAGT 1 cut(s) 3113
SspI AATATT 2 cut(s) 2923, 3402
SstI GAGCTC 2 cut(s) 384, 1751
StyI CCWWGG 7 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254
TaiI ACGT 9 cut(s) 116, 488, 796, 1966, 2615, 3064, 3369, 3619, 4350
TaqII GACCGA 2 cut(s) 1599, 2507
TatI WGTACW 5 cut(s) 445, 629, 2215, 3108, 4000
TauI GCSGC 5 cut(s) 25, 168, 197, 2724, 3081
TscAI CASTG 9 cut(s) 545, 989, 1180, 1339, 1979, 2406, 3516, 3723, 3901
TseFI GTSAC 5 cut(s) 1387, 2401, 3617, 3947, 4343
Tsp45I GTSAC 5 cut(s) 1387, 2401, 3617, 3947, 4343
TspGWI ACGGA 3 cut(s) 462, 3329, 4226
TspRI CASTG 9 cut(s) 545, 989, 1180, 1339, 1979, 2406, 3516, 3723, 3901
Tth111I GACNNNGTC 2 cut(s) 472, 2030
Van91I CCANNNNNTGG 1 cut(s) 3244
Vha464I CTTAAG 1 cut(s) 3442
VpaK11BI GGWCC 5 cut(s) 199, 528, 1582, 2507, 3533
VspI ATTAAT 1 cut(s) 2589
XagI CCTNNNNNAGG 2 cut(s) 3935, 4226
XcmI CCANNNNNNNNNTGG 2 cut(s) 1961, 4160
XmiI GTMKAC 3 cut(s) 207, 963, 2514
XmnI GAANNNNTTC 1 cut(s) 3609
ZraI GACGTC 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 3811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.