Rh7BG237800

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0007888
Physical Location & Seq
Reverse (-)
21031835 .. 21036835
5001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG237800.1

Sequence Viewer

Length: 4365 bp
ATGGCTCCAAAGAAGAAGCAGCCGCAGCAGCAGCCCAAGAAGCCCAGCAGTAAACCCCAACCGGCCTCGTCGGGCCAGAAGCCCCAGCCCAACACCGCCAAACCCCAAACGACGTCCTCCGGCCCCAGGCTCCAGATTTCCGCCGAGAACGAGAACCGCCTCCGCCGCCTCCTCCTCAACTCCGGCCGGTCCGCCGCGGTCCCGGTCGACGAGTCGCTCTCCAAGGCTCAGAAGGCCAAGAAGCTCAGGGCCGTGTACGAGCAGCTCTCCTGCGAAGGCTTCACCAATGATCAGATTGAACTCGCTCTCTCCGCCGTCAAGGAGGGGGCTACATATGAGGCTGCTCTGGACTGGTTGTGCTTAAATGTGCCTGGGAATGAGCTCCCACTCAAGTTTTCGAGTGGTGTTTCGATGGCTGCAAATGAAGGTGGTTCTGTTGGTGTTGTACTGACTTCTCGGGACGACTGGACTCCGTCGGTGGATACGTCTGCCAAGATTGATGAGGATATGCCGGGGATTGCTATTAGGACCAAGGGGCAGTGGAATGATAAAACACTTGATTCATGTCAACCGTCTCAGGCAGATTGGATCAGACGATATGTTGAGCAGCAAGAAGAGGATGAATCGAGTACATGGGAAGATGATGTAGATGATAAGGGTATGGGTGCTAAGGTCAGTAAACCAAGGTCATATGATGTTATAGCCAAAGAGTATCACGCTGCGCGGTTGGAAGCTGCAGAAGCCAAGCAACAAAAGGACAAGAAAAACCAGGAACGGGCAGGCAAAATGATACGTGATCTCAAGCAAGAGTTGGCTGCTCTAGGCTTATCGGATGACATCTTGGCATCAGAATTTGAGCAGGAACAGTCGATAGAACAAGCTTATTCTGCTTTTGAGGACACAGATGCTAGTTCAAAGCCTTACAAGCAGTCTGAAGGACTCCATGCTGATGAATTAAAAGTAGACGGTAATGATATGGAACACTGCAGCTCTGTTCAGCTCCCAACCAACTCTACTCCTTCAGATTTACCTGTTCAGGAAAAAATTGTTGCAGAAGAAGAGACAACTGATATGGAAATTGGTAATTTTTTCTTGGAAGATGCACCATCTAACGAATTCCTAACTCCTACTATTTTGGAACTACAGAAGAAAGAAAAGTTGAGAGAAATGCTCAGTGAGAAAAATTTGGAGAAATTAGATGGTATTTGGAAGAAGGGGGATCCTCAAAAGATTCCCAAGGCTATTTTTCAGCAATTATGTCAAAAATCAGGATGGGAAGCCCCAAAGTTTAATAAAGTGCGGGGAAAGGATAATAGTTTCTCTTATACTATCAGTGTTTTGCGTAAAGCTAGTGGAAGGGGTAAGAGCAGAAAGGCTGGGGGCTTGGTCACTCTTCAGCTTCCTGATCACGATGTAACTTTTGATTCAGCTGAGGATGCACAGAATAGAGTGGCAGCATATGCTCTTTGTCAACTGTTTCCTGATCTCCCAATCCATCTTGTAATTACTGAGCCTTATGCTTCTCTAGTTGTTCAATGGAAGGAAGGGGAAACAATAACCAATGTAGAAGATAGTGTTAAGGACCGAAGAGCTACTTTTGTGGATTCACTATTGAAAGCTGATGGTTCTACTTCAACTTCTGCTGCTAATGTTGTATATGATTCTGATTCTCTTCCTAGAGTGGTTCCACAACTCCAGGTTCAAGAACCTAGAAATGCAGAACTAAATCCTCGCAAAGATGCAGAGAGCTCTTATTTGAAACAAGAACTGGAGAATAAACAGAAAAAGCAGAAATTCAAGGAAATGTTGAGAGCTAGAGCTGCACTCCCCATTGCTGGTTTGAAGGGTGATATATTGCAACTGTTGCAGGATAATAATGTTCTAGTAGTTTGTGGGGAAACGGGATCTGGAAAAACAACTCAGGTTCCACAATTTATATTGGATGACATGATCCAGTCAGGACGTGGTGGACACTGTAACATTATATGCACACAACCAAGGAGAATAGCGGCTATTTCTGTGGCTGACAGAGTCGCTGATGAGCGTTGTGAACCTTCACCTGGTTCAAATGGTTCTTTGGTTGGTTATCAAGTACGCCTTGATAATGCAAGTAATGAAAAGACTAAGCTTCTTTTTTGCACGACAGGTATTCTTCTGAGAAAATTCGTGGGAGACAGAAACTTGACTGGAATTACTCATGTCATAGTTGATGAAGTACATGAGCGGTCTCTTTTGGGTGATTTCCTGCTCATAGTTTTGAAGAATCTGATTGAGAAGCAATCAGCTCTTAACACACCAAAACTGAAAGTTATTCTAATGTCTGCAACTGTTGATTCCAATTTGTTTTCAAATTACTTTGGTGGTTGTCCTGTGATTACTGCAGAAGGTAGAACACATCCAGTGACGACTTATTTTCTTGAGGATATTTATGAGCGCATTGATTATCGGCTTGCATCAGATTCTCCGGCTTCCATGGTCTATGGAAGTTCGACCGAAGGGAAGACTGGTCCTGTAAACAACAGCAGAGGAAAGAAGAATCTTGTTTTGTCTGGCTGGGGTGATGATTCTCTACTCTCCGAAGAATATATTAATCCAAATTATGTTCATGATACGTATCAATCTTACAGAGAGCAAACTAGACAAAATTTGAAAAGATTGAATGAAGATGTCATTGATTATGATCTTCTTGAAGACTTGGTGTGCCATGTTGATGAAACTTGCGGCGAAGGTGCTATTCTTGTCTTCTTGCCTGGAGTATCTGAAATTTACACCTTAGTTGATAAACTAGCTGCTTCTTACCGGTTTGGTGGAGCAGCTTCTGATTGGATCCTCCCGCTACATTCATCTGTTGCATCAGTTGATCAAAAGAAGGTGTTTTTGCAGGCTCCTGAGAACATACGAAAGATTATTGTAGCCACTAATATTGCTGAGACCAGCATAACAATAGATGATGTGGTCTATGTTATTGACTGTGGAAAGCATAAGGAGAATCGTTATAATCCTCAGAAGAAATTGTCGAGCATGGTTGAGGATTGGATATCTAGAGCAAATGCAAGGCAACGTCGAGGACGAGCTGGCCGTGTGAAACCTGGAATTTGCTTTTGCCTGTACACCAGTTATCGATTTGAAAAGCTGATGCGCCCCTTTCAGGTTCCGGAGATGCTTCGAATGCCATTAGTAGAGTTGTGTTTACAAATTAAGTTACTTTCTCTGGGCCACATAAAACCATTTTTGTCCCAGGCTTTGGAACCTCCAAGGGAAGAGGCTATGACTTCAGCAATTAAGATATTGTATGAGGTTGGTGCTCTTGAAACGGATGAAGAGTTAACGCCCCTTGGGCATCATTTGGCGAAACTTCCTGTTGACGTATTGATTGGGAAGATGATGATATATGGTGGAATATTTGGTTGCTTATCACCCATTCTATCAATTTCAGCATTCTTAAGTTACAAATCTCCATTTGTCCATCCCAAAGATGAGAAGGAAAATGCTAAAAGAGCAAAATTGGCACTGTTGACTGACAAGCTAGATGGTCCAAGTGAATCAAATGATGTTGACAAGCAATCTGACCATCTCATAATGATAACTGCATACAAGAAATGGGAAAAGATTCTACGTGACAAAGGGGTTAGAGCTGCGCAGCAGTTTTGCAATTCATATTATTTGAGTAGTTCAGTGATGTACATGATAAGAGACATGAGGATACAGTTTGGAACACTGCTTGCTGATATTGGACTCATTGATCTTCCAAAGAAATATCAGGTTGATGGAAGAAAGAAAGAGAATCTTGACGCTTGGTTTTCGGATGCATCGCAACCCTTCAATATGTATTCAAACCATTCATCAATTGTAAAAGCTATCATATGTGCAGGTTTATACCCTAACATTGCAGCCACTGAGAAAGGCATTGCTGGAACAGTCCTCAATAACCTTAAACAGGCTCCTGGTCACGCAACTAGCCATTGTCCAACCTGGTATGATGGAAGAAGAAAAGTCAATGTACACCCTTCTTCTATCAACCATAATGTAAATGAATTTCGGTATCCTTTCCTTGTCTTCCTCGAAAAGGTTGAAACCAACAAAGTATTTTTGCGGGACTCTACAATCATTTCTCCCAACTCTATTTTGCTGTTTGGTGGCTCCATTAACATCCAGCATCAGACTGGACTTGTTATTGTTGATGGATGGTTAAAACTGACAGCACCAGCACAGACTGCTGTCCTGTTCAAGGAACTCCGTTTAACTCTTCACTCTGTTCTGAAAGAATTGATAAGAAAGCCTGAGAATTGCACTGTTGGTCATAACGAGGTTTTAAGGTCTATTATCCATTTGTTGTTGGAAGAAGACAAGCCTCCTCCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016282 GO:0032991 GO:0043021 GO:0043024 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0070993 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1454

Amino Acids

162.01

Weight (kDa)

6.17

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_DHX29 PF24899 81 - 133 1.7e-17 ATP-dependent RNA helicase DHX29, UBA domain
DEAD PF00270 620 - 780 2e-10 DEAD/DEAH box helicase
Helicase_C PF00271 905 - 1028 1.4e-09 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1093 - 1121 2.7e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1125 - 1193 3.2e-10 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1284 - 1382 9.3e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1389 - 1446 1e-15 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2997
AatII GACGTC 1 cut(s) 116
Acc16I TGCGCA 1 cut(s) 3639
Acc36I ACCTGC 1 cut(s) 3860
AccB7I CCANNNNNTGG 1 cut(s) 3244
AccBSI CCGCTC 1 cut(s) 2224
AccI GTMKAC 2 cut(s) 207, 963
AccII CGCG 2 cut(s) 197, 724
AccIII TCCGGA 1 cut(s) 3154
AclWI GGATC 7 cut(s) 596, 1214, 1227, 1912, 1945, 2821, 2834
AcoI YGGCCR 2 cut(s) 184, 3076
AcsI RAATTY 9 cut(s) 851, 1115, 1183, 1793, 2162, 2644, 2763, 3093, 4034
AcuI CTGAAG 4 cut(s) 954, 1005, 1379, 3258
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 8 cut(s) 257, 447, 631, 2094, 2217, 3110, 3683, 4002
AfiI CCNNNNNNNGG 9 cut(s) 126, 775, 1835, 2060, 3148, 3244, 3937, 4066, 4228
AflII CTTAAG 1 cut(s) 3442
AgeI ACCGGT 1 cut(s) 2799
AjiI CACGTC 1 cut(s) 1964
Alw21I GWGCWC 3 cut(s) 384, 1751, 3307
Alw26I GTCTC 6 cut(s) 579, 1055, 2166, 2232, 2924, 3687
AlwI GGATC 7 cut(s) 596, 1214, 1227, 1912, 1945, 2821, 2834
AlwNI CAGNNNCTG 2 cut(s) 2819, 3896
Ama87I CYCGRG 1 cut(s) 456
Aor13HI TCCGGA 1 cut(s) 3154
AoxI GGCC 8 cut(s) 63, 73, 121, 184, 234, 249, 3076, 3214
ApoI RAATTY 9 cut(s) 851, 1115, 1183, 1793, 2162, 2644, 2763, 3093, 4034
AseI ATTAAT 1 cut(s) 2589
AsiGI ACCGGT 1 cut(s) 2799
Asp700I GAANNNNTTC 1 cut(s) 3609
AspLEI GCGC 4 cut(s) 724, 2436, 3141, 3640
AsuC2I CCSGG 2 cut(s) 203, 513
AsuHPI GGTGA 6 cut(s) 274, 1859, 2049, 2249, 2570, 3408
AsuII TTCGAA 1 cut(s) 3166
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 6 cut(s) 189, 199, 528, 1582, 2507, 3533
BamHI GGATCC 2 cut(s) 1219, 2826
BanII GRGCYC 2 cut(s) 384, 1751
BbsI GAAGAC 5 cut(s) 2507, 2697, 2734, 4048, 4350
Bbv12I GWGCWC 3 cut(s) 384, 1751, 3307
BbvCI CCTCAGC 1 cut(s) 1431
BceAI ACGGC 3 cut(s) 236, 299, 3063
BciVI GTATCC 3 cut(s) 475, 3696, 4053
BclI TGATCA 3 cut(s) 289, 1405, 2860
BcnI CCSGG 2 cut(s) 203, 513
BcoDI GTCTC 6 cut(s) 579, 1055, 2166, 2232, 2924, 3687
BfmI CTRYAG 4 cut(s) 735, 985, 1142, 2379
BfrI CTTAAG 1 cut(s) 3442
BfuAI ACCTGC 1 cut(s) 3860
BfuI GTATCC 3 cut(s) 475, 3696, 4053
BglI GCCNNNNNGGC 1 cut(s) 3337
Bme18I GGWCC 6 cut(s) 189, 199, 528, 1582, 2507, 3533
BmeT110I CYCGRG 1 cut(s) 456
BmgBI CACGTC 1 cut(s) 1964
BoxI GACNNNNGTC 1 cut(s) 4217
BpiI GAAGAC 5 cut(s) 2507, 2697, 2734, 4048, 4350
BplI GAGNNNNNCTC 8 cut(s) 203, 235, 251, 283, 1155, 1187, 1809, 1841
BpmI CTGGAG 4 cut(s) 116, 1679, 1790, 2772
Bpu10I CCTNAGC 3 cut(s) 245, 669, 1431
Bpu14I TTCGAA 1 cut(s) 3166
BpuEI CTTGAG 3 cut(s) 374, 785, 2438
BpuMI CCSGG 2 cut(s) 203, 513
Bsa29I ATCGAT 1 cut(s) 3121
BsaAI YACGTR 3 cut(s) 794, 2613, 3617
BsaBI GATNNNNATC 2 cut(s) 2613, 2679
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 2 cut(s) 2232, 2924
BsaWI WCCGGW 2 cut(s) 2799, 3154
Bsc4I CCNNNNNNNGG 9 cut(s) 126, 775, 1835, 2060, 3148, 3244, 3937, 4066, 4228
Bse118I RCCGGY 3 cut(s) 61, 186, 2799
Bse1I ACTGG 9 cut(s) 356, 470, 1773, 1954, 2191, 2399, 2509, 3114, 4168
Bse3DI GCAATG 3 cut(s) 1830, 3885, 3906
Bse8I GATNNNNATC 2 cut(s) 2613, 2679
BseAI TCCGGA 1 cut(s) 3154
BseCI ATCGAT 1 cut(s) 3121
BseJI GATNNNNATC 2 cut(s) 2613, 2679
BseLI CCNNNNNNNGG 9 cut(s) 126, 775, 1835, 2060, 3148, 3244, 3937, 4066, 4228
BseMI GCAATG 3 cut(s) 1830, 3885, 3906
BseNI ACTGG 9 cut(s) 356, 470, 1773, 1954, 2191, 2399, 2509, 3114, 4168
BseRI GAGGAG 4 cut(s) 161, 164, 4344, 4347
BseX3I CGGCCG 1 cut(s) 184
BseYI CCCAGC 4 cut(s) 44, 84, 1376, 2553
BsgI GTGCAG 2 cut(s) 1806, 3888
Bsh1236I CGCG 2 cut(s) 197, 724
Bsh1285I CGRYCG 3 cut(s) 187, 207, 2493
BshFI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
BshTI ACCGGT 1 cut(s) 2799
BshVI ATCGAT 1 cut(s) 3121
BsiEI CGRYCG 3 cut(s) 187, 207, 2493
BsiHKAI GWGCWC 3 cut(s) 384, 1751, 3307
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 9 cut(s) 62, 120, 183, 187, 203, 512, 2465, 2800, 3155
BslFI GGGAC 4 cut(s) 185, 473, 3220, 4109
BslI CCNNNNNNNGG 9 cut(s) 126, 775, 1835, 2060, 3148, 3244, 3937, 4066, 4228
BsmAI GTCTC 6 cut(s) 579, 1055, 2166, 2232, 2924, 3687
BsmBI CGTCTC 1 cut(s) 579
BsmFI GGGAC 4 cut(s) 185, 473, 3220, 4109
BsmI GAATGC 2 cut(s) 3174, 3437
BsnI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
Bso31I GGTCTC 2 cut(s) 2232, 2924
BsoBI CYCGRG 1 cut(s) 456
Bsp119I TTCGAA 1 cut(s) 3166
Bsp1286I GDGCHC 3 cut(s) 384, 1751, 3307
Bsp13I TCCGGA 1 cut(s) 3154
Bsp1407I TGTACA 3 cut(s) 3108, 3681, 4000
Bsp19I CCATGG 1 cut(s) 2472
BspANI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
BspDI ATCGAT 1 cut(s) 3121
BspEI TCCGGA 1 cut(s) 3154
BspFNI CGCG 2 cut(s) 197, 724
BspHI TCATGA 1 cut(s) 2605
BspMAI CTGCAG 3 cut(s) 739, 989, 2383
BspMI ACCTGC 1 cut(s) 3860
BspPI GGATC 7 cut(s) 596, 1214, 1227, 1912, 1945, 2821, 2834
BspQI GCTCTTC 1 cut(s) 1582
BspT104I TTCGAA 1 cut(s) 3166
BspTI CTTAAG 1 cut(s) 3442
BspTNI GGTCTC 2 cut(s) 2232, 2924
BsrBI CCGCTC 1 cut(s) 2224
BsrDI GCAATG 3 cut(s) 1830, 3885, 3906
BsrFI RCCGGY 3 cut(s) 61, 186, 2799
BsrGI TGTACA 3 cut(s) 3108, 3681, 4000
BsrI ACTGG 9 cut(s) 356, 470, 1773, 1954, 2191, 2399, 2509, 3114, 4168
BssAI RCCGGY 3 cut(s) 61, 186, 2799
BssNI GRCGYC 1 cut(s) 113
BssT1I CCWWGG 8 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254, 3334
Bst6I CTCTTC 8 cut(s) 609, 1053, 1398, 1582, 1677, 3255, 3315, 4251
BstACI GRCGYC 1 cut(s) 113
BstAFI CTTAAG 1 cut(s) 3442
BstAPI GCANNNNNTGC 3 cut(s) 1460, 1864, 4214
BstAUI TGTACA 3 cut(s) 3108, 3681, 4000
BstBAI YACGTR 3 cut(s) 794, 2613, 3617
BstBI TTCGAA 1 cut(s) 3166
BstC8I GCNNGC 5 cut(s) 781, 2451, 2883, 3076, 3723
BstDSI CCRYGG 2 cut(s) 195, 2472
BstENI CCTNNNNNAGG 3 cut(s) 3935, 4064, 4226
BstFNI CGCG 2 cut(s) 197, 724
BstHHI GCGC 4 cut(s) 724, 2436, 3141, 3640
BstMAI GTCTC 6 cut(s) 579, 1055, 2166, 2232, 2924, 3687
BstMCI CGRYCG 3 cut(s) 187, 207, 2493
BstPAI GACNNNNGTC 1 cut(s) 4217
BstSFI CTRYAG 4 cut(s) 735, 985, 1142, 2379
BstSNI TACGTA 1 cut(s) 2613
BstUI CGCG 2 cut(s) 197, 724
BstV2I GAAGAC 5 cut(s) 2507, 2697, 2734, 4048, 4350
BstX2I RGATCY 3 cut(s) 1219, 1904, 2826
BstYI RGATCY 3 cut(s) 1219, 1904, 2826
BstZI CGGCCG 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 3121
BsuI GTATCC 3 cut(s) 475, 3696, 4053
BsuRI GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
BsuTUI ATCGAT 1 cut(s) 3121
BtgI CCRYGG 2 cut(s) 195, 2472
BtgZI GCGATG 1 cut(s) 3795
BtrI CACGTC 1 cut(s) 1964
BtsI GCAGTG 3 cut(s) 545, 982, 3716
BveI ACCTGC 1 cut(s) 3860
Cac8I GCNNGC 5 cut(s) 781, 2451, 2883, 3076, 3723
CaiI CAGNNNCTG 2 cut(s) 2819, 3896
CciI TCATGA 1 cut(s) 2605
CfoI GCGC 4 cut(s) 724, 2436, 3141, 3640
Cfr10I RCCGGY 3 cut(s) 61, 186, 2799
Cfr42I CCGCGG 1 cut(s) 198
ClaI ATCGAT 1 cut(s) 3121
CpoI CGGWCCG 1 cut(s) 189
CseI GACGC 1 cut(s) 3800
CsiI ACCWGGT 2 cut(s) 2059, 3971
Csp6I GTAC 8 cut(s) 256, 446, 630, 2093, 2216, 3109, 3682, 4001
CspAI ACCGGT 1 cut(s) 2799
CspCI CAANNNNNGTGG 2 cut(s) 2905, 2940
CspI CGGWCCG 1 cut(s) 189
CviQI GTAC 8 cut(s) 256, 446, 630, 2093, 2216, 3109, 3682, 4001
EaeI YGGCCR 2 cut(s) 184, 3076
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 8 cut(s) 609, 1053, 1398, 1582, 1677, 3255, 3315, 4251
EarI CTCTTC 8 cut(s) 609, 1053, 1398, 1582, 1677, 3255, 3315, 4251
EciI GGCGGA 4 cut(s) 130, 152, 181, 301
Ecl136II GAGCTC 2 cut(s) 382, 1749
EclXI CGGCCG 1 cut(s) 184
Eco105I TACGTA 1 cut(s) 2613
Eco130I CCWWGG 8 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254, 3334
Eco24I GRGCYC 2 cut(s) 384, 1751
Eco31I GGTCTC 2 cut(s) 2232, 2924
Eco32I GATATC 1 cut(s) 3039
Eco47I GGWCC 6 cut(s) 189, 199, 528, 1582, 2507, 3533
Eco52I CGGCCG 1 cut(s) 184
Eco53kI GAGCTC 2 cut(s) 382, 1749
Eco57I CTGAAG 4 cut(s) 954, 1005, 1379, 3258
Eco88I CYCGRG 1 cut(s) 456
EcoICRI GAGCTC 2 cut(s) 382, 1749
EcoNI CCTNNNNNAGG 3 cut(s) 3935, 4064, 4226
EcoRI GAATTC 1 cut(s) 1115
EcoRV GATATC 1 cut(s) 3039
EcoT14I CCWWGG 8 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254, 3334
EcoT22I ATGCAT 1 cut(s) 3811
EcoT38I GRGCYC 2 cut(s) 384, 1751
ErhI CCWWGG 8 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254, 3334
Esp3I CGTCTC 1 cut(s) 579
FalI AAGNNNNNCTT 6 cut(s) 1579, 1611, 2082, 2114, 3771, 3803
FaqI GGGAC 4 cut(s) 185, 473, 3220, 4109
FauI CCCGC 3 cut(s) 1293, 2841, 4086
FauNDI CATATG 4 cut(s) 334, 691, 1459, 3863
FbaI TGATCA 3 cut(s) 289, 1405, 2860
FblI GTMKAC 2 cut(s) 207, 963
FriOI GRGCYC 2 cut(s) 384, 1751
FspI TGCGCA 1 cut(s) 3639
GlaI GCGC 4 cut(s) 723, 2435, 3140, 3639
GsaI CCCAGC 4 cut(s) 48, 88, 1380, 2557
GsuI CTGGAG 4 cut(s) 116, 1679, 1790, 2772
HaeIII GGCC 8 cut(s) 65, 75, 123, 186, 236, 251, 3078, 3216
HapII CCGG 9 cut(s) 62, 120, 183, 187, 203, 512, 2465, 2800, 3155
HgaI GACGC 1 cut(s) 3800
HhaI GCGC 4 cut(s) 724, 2436, 3141, 3640
Hin1I GRCGYC 1 cut(s) 113
Hin6I GCGC 4 cut(s) 722, 2434, 3139, 3638
HinP1I GCGC 4 cut(s) 722, 2434, 3139, 3638
HincII GTYRAC 7 cut(s) 208, 569, 1472, 3327, 3364, 3516, 3556
HindII GTYRAC 7 cut(s) 208, 569, 1472, 3327, 3364, 3516, 3556
HindIII AAGCTT 2 cut(s) 879, 2126
HpaI GTTAAC 1 cut(s) 3327
HpaII CCGG 9 cut(s) 62, 120, 183, 187, 203, 512, 2465, 2800, 3155
HphI GGTGA 6 cut(s) 274, 1859, 2049, 2249, 2570, 3408
Hpy99I CGWCG 5 cut(s) 73, 115, 212, 478, 3066
HpyCH4IV ACGT 8 cut(s) 113, 485, 793, 1963, 2612, 3061, 3366, 3616
HpySE526I ACGT 8 cut(s) 113, 485, 793, 1963, 2612, 3061, 3366, 3616
Hsp92I GRCGYC 1 cut(s) 113
HspAI GCGC 4 cut(s) 722, 2434, 3139, 3638
Kpn2I TCCGGA 1 cut(s) 3154
Ksp22I TGATCA 3 cut(s) 289, 1405, 2860
KspAI GTTAAC 1 cut(s) 3327
KspI CCGCGG 1 cut(s) 198
LguI GCTCTTC 1 cut(s) 1582
LmnI GCTCC 8 cut(s) 10, 135, 387, 1005, 2810, 2890, 3946, 4145
MabI ACCWGGT 2 cut(s) 2059, 3971
MaeII ACGT 8 cut(s) 113, 485, 793, 1963, 2612, 3061, 3366, 3616
MaeIII GTNAC 8 cut(s) 1387, 1414, 1976, 2401, 3201, 3446, 3617, 3947
MbiI CCGCTC 1 cut(s) 2224
MfeI CAATTG 1 cut(s) 3846
MflI RGATCY 3 cut(s) 1219, 1904, 2826
MhlI GDGCHC 3 cut(s) 384, 1751, 3307
MlyI GAGTC 6 cut(s) 221, 463, 933, 2040, 3729, 4091
MmeI TCCRAC 3 cut(s) 708, 3992, 4317
Mph1103I ATGCAT 1 cut(s) 3811
MroI TCCGGA 1 cut(s) 3154
MroXI GAANNNNTTC 1 cut(s) 3609
MslI CAYNNNNRTG 2 cut(s) 948, 2709
MspA1I CMGCKG 2 cut(s) 197, 1430
MspCI CTTAAG 1 cut(s) 3442
MspI CCGG 9 cut(s) 62, 120, 183, 187, 203, 512, 2465, 2800, 3155
MunI CAATTG 1 cut(s) 3846
Mva1269I GAATGC 2 cut(s) 3174, 3437
MvnI CGCG 2 cut(s) 197, 724
NciI CCSGG 2 cut(s) 203, 513
NcoI CCATGG 1 cut(s) 2472
NdeI CATATG 4 cut(s) 334, 691, 1459, 3863
NmeAIII GCCGAG 1 cut(s) 169
NmuCI GTSAC 4 cut(s) 1387, 2401, 3617, 3947
NsbI TGCGCA 1 cut(s) 3639
NsiI ATGCAT 1 cut(s) 3811
NspV TTCGAA 1 cut(s) 3166
PagI TCATGA 1 cut(s) 2605
PciSI GCTCTTC 1 cut(s) 1582
PcsI WCGNNNNNNNCGW 3 cut(s) 482, 3067, 3076
PctI GAATGC 2 cut(s) 3174, 3437
PdmI GAANNNNTTC 1 cut(s) 3609
PflFI GACNNNGTC 2 cut(s) 472, 2030
PflMI CCANNNNNTGG 1 cut(s) 3244
PinAI ACCGGT 1 cut(s) 2799
PleI GAGTC 6 cut(s) 220, 463, 933, 2039, 3729, 4091
PpsI GAGTC 6 cut(s) 220, 463, 933, 2039, 3729, 4091
Ppu21I YACGTR 3 cut(s) 794, 2613, 3617
PshAI GACNNNNGTC 1 cut(s) 4217
PshBI ATTAAT 1 cut(s) 2589
PsiI TTATAA 1 cut(s) 2997
Psp124BI GAGCTC 2 cut(s) 384, 1751
PspFI CCCAGC 4 cut(s) 44, 84, 1376, 2553
PstI CTGCAG 3 cut(s) 739, 989, 2383
PstNI CAGNNNCTG 2 cut(s) 2819, 3896
PsuI RGATCY 3 cut(s) 1219, 1904, 2826
PsyI GACNNNGTC 2 cut(s) 472, 2030
PvuII CAGCTG 1 cut(s) 1430
RsaI GTAC 8 cut(s) 257, 447, 631, 2094, 2217, 3110, 3683, 4002
RsaNI GTAC 8 cut(s) 256, 446, 630, 2093, 2216, 3109, 3682, 4001
RseI CAYNNNNRTG 2 cut(s) 948, 2709
Rsr2I CGGWCCG 1 cut(s) 189
RsrII CGGWCCG 1 cut(s) 189
SacI GAGCTC 2 cut(s) 384, 1751
SacII CCGCGG 1 cut(s) 198
SalI GTCGAC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 1582
SchI GAGTC 6 cut(s) 221, 463, 933, 2040, 3729, 4091
SduI GDGCHC 3 cut(s) 384, 1751, 3307
SexAI ACCWGGT 2 cut(s) 2059, 3971
SfcI CTRYAG 4 cut(s) 735, 985, 1142, 2379
Sfr303I CCGCGG 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 3166
SgrBI CCGCGG 1 cut(s) 198
SinI GGWCC 6 cut(s) 189, 199, 528, 1582, 2507, 3533
SmiMI CAYNNNNRTG 2 cut(s) 948, 2709
SmlI CTYRAG 4 cut(s) 389, 800, 2417, 3442
SmoI CTYRAG 4 cut(s) 389, 800, 2417, 3442
SnaBI TACGTA 1 cut(s) 2613
SspI AATATT 2 cut(s) 2923, 3402
SstI GAGCTC 2 cut(s) 384, 1751
StyI CCWWGG 8 cut(s) 222, 531, 683, 1236, 1997, 2472, 3254, 3334
TaiI ACGT 8 cut(s) 116, 488, 796, 1966, 2615, 3064, 3369, 3619
TaqII GACCGA 2 cut(s) 1599, 2507
TatI WGTACW 6 cut(s) 445, 629, 2215, 3108, 3681, 4000
TauI GCSGC 5 cut(s) 25, 168, 197, 2012, 2724
TseFI GTSAC 4 cut(s) 1387, 2401, 3617, 3947
Tsp45I GTSAC 4 cut(s) 1387, 2401, 3617, 3947
TspGWI ACGGA 3 cut(s) 462, 3329, 4226
Tth111I GACNNNGTC 2 cut(s) 472, 2030
Van91I CCANNNNNTGG 1 cut(s) 3244
Vha464I CTTAAG 1 cut(s) 3442
VpaK11BI GGWCC 6 cut(s) 189, 199, 528, 1582, 2507, 3533
VspI ATTAAT 1 cut(s) 2589
XagI CCTNNNNNAGG 3 cut(s) 3935, 4064, 4226
XapI RAATTY 9 cut(s) 851, 1115, 1183, 1793, 2162, 2644, 2763, 3093, 4034
XbaI TCTAGA 1 cut(s) 3041
XcmI CCANNNNNNNNNTGG 2 cut(s) 1961, 4160
XmiI GTMKAC 2 cut(s) 207, 963
XmnI GAANNNNTTC 1 cut(s) 3609
ZraI GACGTC 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 3811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.