FvH4_5g21580

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007888
Physical Location & Seq
Reverse (-)
13015073 .. 13020955
5883 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21580.t1

Sequence Viewer

Length: 4371 bp
ATGCCTCCAAAGAAGAAGCAGCCGCAGCAGCAGCAGTCTAAGAAGCCGAGCAGTAAACCCCAACCGTCCTCCTCGGGCCAGAAGCCCCAGCCAAACACCACCGCCAAACCCCAAACGACGTCGTCCGGCCCCCGCCTCCAGATTTCCGCCGAGAACGAGAACCGCCTCCGCCGCCTCCTCCTCAACTCCGGCCGTTCCGCCGCCGTCCCCGTCGACGAGTCGCTCTCCAAGGCTCAGAAGGCCAAGAAGCTCAAGGCCGTCTACGAGCAGCTCTCCTGCGAAGGCTTCACCAATGATCAGATTGAGCTCGCTCTCTCCGCCGTCAAGGAGGGGGCTACATATGAGGCTGCTCTGGATTGGTTGTGCTTAAATGTGCCTGGGGATGAGCTTCCATTGAAGTTTTCGAGTGGGGTTTCCATGGCTGCAAATGAAGGTGGCTCTGTTGGTGTTGTACTGACTTCTCGAGATGACTGGACTCCGTCGGTGGACACGTCTGCTAAGATTGATGAGGATACGCCGGGGATTGCTATTAGAACCAAGGGGCAATGGGATGATAAAACACTTGATTCGTGTCAACCGTCTCAGGCAGATTGGATCAAGCGGTATGTTGAGCAGCAAGAAGAGGATGAATCGAGTACGTGGGAGGATGATGTAGATGATGAGGTTTCGGGGGCTAAGGTCAGGAAACCAAGGTCGTATGATGTTATAGCCAAGGAGTATCATGCTGCGCGGTTAGAAGCTGCGGAAGCGAAGCAAAAGAAGGACAAGAAAAACCAGGAACGGGCAGGCAAAGTAATACGTGATCTCAAGCAAGAGTTATCGGCACTAGGCTTATCGGACGACATCTTGGCATCAGAATTTGAGCAGGAACAGTCTATAGAACGAGCTTATTCTGCTTTTGAGGACACAGATACTAGTTCAGAGCCTTACAAGCAAGCTGATGGACTCCATGCAGATGAATTAAAAGCAGACGGAAATGATATGGAGCCTTGCAGCTCCGTTCAGCTCCCAATCAACTCTACTCCTTCAGATCTACCTGTTCAGGAAAAAATTGCTGCAGAAGAAGAAACAACTGATATGGAGATTGGTAATTTTTTCTTGGAAGATGCGCCGTCTAACGATTTCCTAACTCCTACTATTTTGGAACTACAGAAGAAAGAAAAGTTGAGAGAAATGCTCAGTGAGAAAAATTTGGAGAAATTAGATGGTATTTGGAAGAAAGGGGAGCCTCAAAAGATTCCCAAGGCTGTATTTCATCAATTATGTCAAAAATCAGGATGGGAAGCCCCAAAGTTTAATAAAGTGCGGGGCAAGGATAATAGTTTCTCTTATACTATCAGTGTTTTGCGTAAAGCTAGTGGAAGGGGTAAGAGCAGAAAGGCTGGGGGCTTGGTCACTCTTCAGCTTCCTGATCACGATGTAACTTTTGATTCAGCCGAGGATGCACAGAATAGAGTGGCAGCATATGCTCTTTGTCAACTGTTTAATGATCTCCCAATCCATCTTGTTATTACTGAGCCTTATGCTTCTCTAATCATTCAATGGAAGGAAGGGGAAACTATGACCAATGTTGAAGATGGTGTTAAGGACCGAAGAGCTAATTTTGTGGATTCGCTATTGAAAGCTGATGGTTCTAGTTCAACTTCTACTGCTAATGTTGTGTATGATTCTGATTCTCTTCCTAAAGTGGTTCCACGACTCCAGGTTCAAGAACCTAGAAATTCAGAACTAAATCCTCGCAAAGATGCAGAGAGCTCTTATTTGAGACAAGAACTGGAGAATAAACAGAAAACACAGAAATTCAAGGAAATGTTGAAAGCAAGAGCTGCACTCCCCATTGCTGGTTTGAAGGGTGATATATTGCAACTGCTTCAGGATAATAATGTTCTAGTAGTTTGTGGTGAAACGGGATCTGGAAAAACAACTCAGGTTCCACAATTTATATTGGATGACATGATCCAGTCAGGACGTGGTGGACACTGTAACATTATATGCACACAACCAAGGAGAATAGCTGCTATTTCTGTGGCTGACAGAGTCACTGATGAGCGTTGTGAACCCTCACCTGGTTCTAATGGTTCTTTGGTTGGTTATCAAGTGCGCCTTGATAATGCTAGTAATGAAAAGACTAAGCTTCTGTTTTGCACGACAGGTATTCTTCTGAGAAAATTCGTGGGAGACAGAAACTTGACTGGAGTTACTCATGTCATAGTTGATGAAGTACATGAGCGGTCTCTTTTGGGTGATTTCCTGCTCATAGTTTTGAAGAATCTGATTGAGAAGCAATCAGCTCTTAACACACCCAAACTGAAAGTTATTCTAATGTCTGCAACTGTTGATTCCAATTTGTTTTCAAATTACTTTGGTGGTTGTCCTGTGATTACTGCAGAAGGTAGAACACATCCAGTGACAACTTATTATCTTGAGGATATATATGAGCGCATTGATTATCGGCTTGCATCAGATTCTCCGGCTTCCATGGTGTATGGAACTTCGACCGAAGGGAAGACTGGTCCTGTAAACAACAGCAGAGGAAAGAAGAATCTTGTTTTATCTGGCTGGGGTGATGATTCTGTACTGTCCGAAGAATTTGTTAATCCAAATTATGTTCATGATATGTATCAATCCTACAGAGAGCAAACTCGACAAAATTTGAAAAGATTGAATGAAGATGTCATTGATTATGATCTTCTTGAAGACTTGGTGTGCCATGTTGATGAAACTTGCGCTGAAGGTGCTGTTCTTGTCTTCTTGCCTGGAGTATCTGAAATTTACACCTTAGTTGATAAACTAGCTGCTTCTTACCGGTTCGGTGGAGCAGCTTCTGATTGGATCCTCCCGCTACATTCATCTGTTGCATCAGTTGATCAAAAGAAGGTATTTTTGCAGGCTCCTGATAATATACGAAAGATTATTGTAGCTACTAATATTGCTGAGACCAGCATAACAATAGATGATGTGGTATATGTTATTGACTGTGGAAAGCATAAGGAGAATCGTTATAATCCTCAGAAGAAATTGTCAAGCATGGTCGAGGATTGGATATCCAAAGCAAATGCAAGGCAACGTCGAGGAAGAGCTGGACGTGTGAAACCTGGAATCTGCTTTTGCATGTACACTAGTTATCGATTTGAAAAGCTCATGCGCCCCTTTCAGGTTCCGGAGATGCTTCGAATGCCATTAGTAGAGCTGTGTTTACAAATTAAGTTACTTTCTTTGGGCCACATAAAACCATTTTTGTCCCAGGCTTTAGAACCTCCAAGGGAAGAGGCTATGACTTCAGCAATTAAGATATTGTATGAGGTTGGTGCTCTTGAAACAGATGAAGAGTTGACGCCTCTTGGGCATCATTTGGCGAAACTTCCTGTTGACGTATTGATTGGGAAGATGATGATACATGGTGGAATATTTGGTTGCTTATCACCGATTCTATCAATTTCTGCATTCTTAAGTTACAAATCTCCATTTGTCCATCCAAAAGATGAGAAGGAAAATGCGAAAAGGGCAAAATTGGCACTGTTGACTGACAAGCTAGATGGACCAAGTGAATCAAACAATGTTGATAAGCAATCCGACCATCTCATAATGATAACCGCATACAAGAAGTGGGAAAAGATTCTACGTGACAAAGGGGTTAGAGCTGCTCAACAGTTTTGCAGTTCATATTTTTTGAGCAGTTCAGTGATGTACATGATAAGAGACATGAGGATACAGTTTGGAACACTGCTGGCTGATATTGGACTCATTGATCTTCCAAAGAAATATCAGGTTGATGGAAGAAAGAAAGAGAATCTAGACGCTTGGTTTTCAGATGCATCACAACCCTTTAATATGTACTCAAACCATTCACCAATTGTGAAAGCTATCATATGTGCAGGGTTATACCCCAATGTTGCAGCCACTGAGAAAGGCATTGCTGGAACAGTTCTCAATAACCTTAAACAGGCTCCTGGCCACGCAGCTAGCCATTGTCCAACCTGGTATGATGGAAGAAGAAAAGTCAATATACACCCTTCGTCTATCAACCATAATGTAAATGAATTCCGGTACCCTTTCCTTGTCTTCCTTGAAAAGGTTGAAACCAACAAAGTGTTTTTGCGGGACTCTACAATCATTTCCCCCAACTCTATTTTGCTGTTTGGTGGCTCCATTAACATCCAGCATCAGACCGGACTTGTTATTGTCGATGGATGGTTAAAACTGACAGCCCCAGCACAAACTGCTGTCCTGTTCAAGGAACTCCGTTTAACTCTTCACTCTGTTCTAAAGGAATTGATAAGAAAGCCAGAGAATTGCACCGTTGGTCATAACGAGGTTCTAAGGTCTATTATCCATTTGTTGTTGGAAGAAGACAAGCCTCCTCCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016282 GO:0032991 GO:0043021 GO:0043024 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0070993 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1457

Amino Acids

162.15

Weight (kDa)

6.1

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_DHX29 PF24899 83 - 135 6.7e-18 ATP-dependent RNA helicase DHX29, UBA domain
DSRM_DHX29 PF24385 412 - 516 1.5e-06 ATP-dependent RNA helicase DHX29, DSRM-like
DEAD PF00270 622 - 782 1.1e-10 DEAD/DEAH box helicase
Helicase_C PF00271 907 - 1030 8.3e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1095 - 1123 2.7e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1127 - 1195 9.6e-10 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1286 - 1384 4.5e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1391 - 1448 1e-15 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3003
AatII GACGTC 1 cut(s) 122
Acc65I GGTACC 1 cut(s) 4047
AccB1I GGYRCC 1 cut(s) 4047
AccBSI CCGCTC 1 cut(s) 2230
AccI GTMKAC 2 cut(s) 213, 261
AccII CGCG 1 cut(s) 730
AccIII TCCGGA 1 cut(s) 3160
AclWI GGATC 5 cut(s) 602, 1918, 1951, 2827, 2840
AcoI YGGCCR 2 cut(s) 190, 3952
AcsI RAATTY 9 cut(s) 857, 1189, 1720, 1799, 2168, 2588, 2650, 2769, 4040
AcuI CTGAAG 5 cut(s) 1011, 1385, 1856, 2751, 3264
AcyI GRCGYC 2 cut(s) 119, 3335
AfaI GTAC 8 cut(s) 453, 637, 2223, 2577, 3116, 3689, 3836, 4049
AfiI CCNNNNNNNGG 7 cut(s) 781, 1841, 2066, 3154, 3943, 4072, 4234
AflII CTTAAG 1 cut(s) 3448
AflIII ACRYGT 2 cut(s) 489, 3085
AgeI ACCGGT 1 cut(s) 2805
AhlI ACTAGT 2 cut(s) 914, 3119
AjiI CACGTC 3 cut(s) 492, 1970, 3086
AjnI CCWGG 9 cut(s) 376, 774, 1701, 2065, 2755, 3094, 3243, 3949, 3977
Alw21I GWGCWC 3 cut(s) 309, 1757, 3313
Alw26I GTCTC 6 cut(s) 585, 1759, 2172, 2238, 2930, 3693
AlwI GGATC 5 cut(s) 602, 1918, 1951, 2827, 2840
AlwNI CAGNNNCTG 2 cut(s) 2825, 3902
Ama87I CYCGRG 2 cut(s) 73, 462
Aor13HI TCCGGA 1 cut(s) 3160
AoxI GGCC 7 cut(s) 76, 127, 190, 240, 255, 3220, 3952
ApoI RAATTY 9 cut(s) 857, 1189, 1720, 1799, 2168, 2588, 2650, 2769, 4040
AsiGI ACCGGT 1 cut(s) 2805
Asp700I GAANNNNTTC 1 cut(s) 3615
Asp718I GGTACC 1 cut(s) 4047
AspLEI GCGC 6 cut(s) 730, 1111, 2103, 2442, 2729, 3147
AspS9I GGNCC 6 cut(s) 76, 128, 1588, 2513, 3220, 3539
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 8 cut(s) 280, 1865, 1913, 2055, 2255, 2576, 3414, 3840
AsuII TTCGAA 1 cut(s) 3172
AsuNHI GCTAGC 1 cut(s) 3962
AvaI CYCGRG 2 cut(s) 73, 462
AvaII GGWCC 3 cut(s) 1588, 2513, 3539
BalI TGGCCA 1 cut(s) 3954
BamHI GGATCC 1 cut(s) 2832
BanI GGYRCC 1 cut(s) 4047
BanII GRGCYC 2 cut(s) 309, 1757
BbsI GAAGAC 5 cut(s) 2513, 2703, 2740, 4054, 4356
Bbv12I GWGCWC 3 cut(s) 309, 1757, 3313
BceAI ACGGC 5 cut(s) 177, 188, 242, 305, 1096
BciT130I CCWGG 9 cut(s) 378, 776, 1703, 2067, 2757, 3096, 3245, 3951, 3979
BciVI GTATCC 2 cut(s) 505, 3702
BclI TGATCA 3 cut(s) 295, 1411, 2866
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 6 cut(s) 585, 1759, 2172, 2238, 2930, 3693
BcuI ACTAGT 2 cut(s) 914, 3119
BfmI CTRYAG 5 cut(s) 876, 1056, 1148, 2385, 2629
BfrI CTTAAG 1 cut(s) 3448
BfuI GTATCC 2 cut(s) 505, 3702
BglI GCCNNNNNGGC 1 cut(s) 3343
BglII AGATCT 1 cut(s) 1030
Bme18I GGWCC 3 cut(s) 1588, 2513, 3539
BmeT110I CYCGRG 2 cut(s) 73, 462
BmgBI CACGTC 3 cut(s) 492, 1970, 3086
BmgT120I GGNCC 6 cut(s) 76, 128, 1588, 2513, 3220, 3539
BmtI GCTAGC 1 cut(s) 3966
BpiI GAAGAC 5 cut(s) 2513, 2703, 2740, 4054, 4356
BplI GAGNNNNNCTC 8 cut(s) 209, 241, 257, 289, 1161, 1193, 1815, 1847
BpmI CTGGAG 5 cut(s) 122, 1685, 1796, 2214, 2778
Bpu10I CCTNAGC 1 cut(s) 675
Bpu14I TTCGAA 1 cut(s) 3172
BpuEI CTTGAG 3 cut(s) 236, 791, 2444
BpuMI CCSGG 1 cut(s) 519
Bsa29I ATCGAT 1 cut(s) 3127
BsaAI YACGTR 3 cut(s) 639, 800, 3623
BsaBI GATNNNNATC 2 cut(s) 2619, 2685
BsaHI GRCGYC 2 cut(s) 119, 3335
BsaI GGTCTC 2 cut(s) 2238, 2930
BsaWI WCCGGW 4 cut(s) 2805, 3160, 4044, 4169
Bsc4I CCNNNNNNNGG 7 cut(s) 781, 1841, 2066, 3154, 3943, 4072, 4234
Bse118I RCCGGY 1 cut(s) 2805
Bse1I ACTGG 6 cut(s) 476, 1779, 1960, 2197, 2405, 2515
Bse3DI GCAATG 3 cut(s) 551, 1836, 3912
Bse8I GATNNNNATC 2 cut(s) 2619, 2685
BseAI TCCGGA 1 cut(s) 3160
BseBI CCWGG 9 cut(s) 378, 776, 1703, 2067, 2757, 3096, 3245, 3951, 3979
BseCI ATCGAT 1 cut(s) 3127
BseJI GATNNNNATC 2 cut(s) 2619, 2685
BseLI CCNNNNNNNGG 7 cut(s) 781, 1841, 2066, 3154, 3943, 4072, 4234
BseMI GCAATG 3 cut(s) 551, 1836, 3912
BseMII CTCAG 9 cut(s) 248, 596, 1192, 1506, 1940, 2153, 2925, 3023, 3894
BseNI ACTGG 6 cut(s) 476, 1779, 1960, 2197, 2405, 2515
BseRI GAGGAG 5 cut(s) 61, 167, 170, 4350, 4353
BseX3I CGGCCG 1 cut(s) 190
BseYI CCCAGC 4 cut(s) 87, 1382, 2559, 4210
BsgI GTGCAG 2 cut(s) 1812, 3894
Bsh1236I CGCG 1 cut(s) 730
Bsh1285I CGRYCG 2 cut(s) 193, 2499
BshFI GGCC 7 cut(s) 78, 129, 192, 242, 257, 3222, 3954
BshNI GGYRCC 1 cut(s) 4047
BshTI ACCGGT 1 cut(s) 2805
BshVI ATCGAT 1 cut(s) 3127
BsiEI CGRYCG 2 cut(s) 193, 2499
BsiHKAI GWGCWC 3 cut(s) 309, 1757, 3313
BsiHKCI CYCGRG 2 cut(s) 73, 462
BsiSI CCGG 8 cut(s) 126, 189, 518, 2471, 2806, 3161, 4045, 4170
BslFI GGGAC 3 cut(s) 191, 3226, 4115
BslI CCNNNNNNNGG 7 cut(s) 781, 1841, 2066, 3154, 3943, 4072, 4234
BsmAI GTCTC 6 cut(s) 585, 1759, 2172, 2238, 2930, 3693
BsmBI CGTCTC 1 cut(s) 585
BsmFI GGGAC 3 cut(s) 191, 3226, 4115
BsmI GAATGC 2 cut(s) 3180, 3443
BsnI GGCC 7 cut(s) 78, 129, 192, 242, 257, 3222, 3954
Bso31I GGTCTC 2 cut(s) 2238, 2930
BsoBI CYCGRG 2 cut(s) 73, 462
Bsp119I TTCGAA 1 cut(s) 3172
Bsp1286I GDGCHC 3 cut(s) 309, 1757, 3313
Bsp13I TCCGGA 1 cut(s) 3160
Bsp1407I TGTACA 2 cut(s) 3114, 3687
Bsp19I CCATGG 2 cut(s) 417, 2478
BspANI GGCC 7 cut(s) 78, 129, 192, 242, 257, 3222, 3954
BspCNI CTCAG 9 cut(s) 247, 595, 1191, 1507, 1939, 2154, 2926, 3022, 3895
BspDI ATCGAT 1 cut(s) 3127
BspEI TCCGGA 1 cut(s) 3160
BspFNI CGCG 1 cut(s) 730
BspHI TCATGA 1 cut(s) 2611
BspMAI CTGCAG 2 cut(s) 1060, 2389
BspOI GCTAGC 1 cut(s) 3966
BspPI GGATC 5 cut(s) 602, 1918, 1951, 2827, 2840
BspQI GCTCTTC 2 cut(s) 1588, 3070
BspT104I TTCGAA 1 cut(s) 3172
BspT107I GGYRCC 1 cut(s) 4047
BspTI CTTAAG 1 cut(s) 3448
BspTNI GGTCTC 2 cut(s) 2238, 2930
BsrBI CCGCTC 1 cut(s) 2230
BsrDI GCAATG 3 cut(s) 551, 1836, 3912
BsrFI RCCGGY 1 cut(s) 2805
BsrGI TGTACA 2 cut(s) 3114, 3687
BsrI ACTGG 6 cut(s) 476, 1779, 1960, 2197, 2405, 2515
BssAI RCCGGY 1 cut(s) 2805
BssNI GRCGYC 2 cut(s) 119, 3335
BssT1I CCWWGG 9 cut(s) 228, 417, 537, 689, 711, 1242, 2003, 2478, 3260
Bst2UI CCWGG 9 cut(s) 378, 776, 1703, 2067, 2757, 3096, 3245, 3951, 3979
Bst6I CTCTTC 8 cut(s) 615, 1404, 1588, 1683, 3070, 3261, 3321, 4257
BstACI GRCGYC 2 cut(s) 119, 3335
BstAFI CTTAAG 1 cut(s) 3448
BstAPI GCANNNNNTGC 3 cut(s) 1466, 1826, 4220
BstAUI TGTACA 2 cut(s) 3114, 3687
BstBAI YACGTR 3 cut(s) 639, 800, 3623
BstBI TTCGAA 1 cut(s) 3172
BstC8I GCNNGC 7 cut(s) 309, 787, 936, 2457, 2889, 3729, 3964
BstDSI CCRYGG 2 cut(s) 417, 2478
BstENI CCTNNNNNAGG 3 cut(s) 3941, 4070, 4232
BstFNI CGCG 1 cut(s) 730
BstHHI GCGC 6 cut(s) 730, 1111, 2103, 2442, 2729, 3147
BstMAI GTCTC 6 cut(s) 585, 1759, 2172, 2238, 2930, 3693
BstMCI CGRYCG 2 cut(s) 193, 2499
BstNI CCWGG 9 cut(s) 378, 776, 1703, 2067, 2757, 3096, 3245, 3951, 3979
BstNSI RCATGY 1 cut(s) 3115
BstSFI CTRYAG 5 cut(s) 876, 1056, 1148, 2385, 2629
BstUI CGCG 1 cut(s) 730
BstV2I GAAGAC 5 cut(s) 2513, 2703, 2740, 4054, 4356
BstX2I RGATCY 3 cut(s) 1030, 1910, 2832
BstYI RGATCY 3 cut(s) 1030, 1910, 2832
BstZI CGGCCG 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 3127
BsuI GTATCC 2 cut(s) 505, 3702
BsuRI GGCC 7 cut(s) 78, 129, 192, 242, 257, 3222, 3954
BsuTUI ATCGAT 1 cut(s) 3127
BtgI CCRYGG 2 cut(s) 417, 2478
BtrI CACGTC 3 cut(s) 492, 1970, 3086
BtsI GCAGTG 1 cut(s) 3722
BtsIMutI CAGTG 9 cut(s) 1186, 1345, 1978, 2040, 2412, 3515, 3687, 3722, 3900
Cac8I GCNNGC 7 cut(s) 309, 787, 936, 2457, 2889, 3729, 3964
CaiI CAGNNNCTG 2 cut(s) 2825, 3902
CciI TCATGA 1 cut(s) 2611
CfoI GCGC 6 cut(s) 730, 1111, 2103, 2442, 2729, 3147
Cfr10I RCCGGY 1 cut(s) 2805
Cfr13I GGNCC 6 cut(s) 76, 128, 1588, 2513, 3220, 3539
ClaI ATCGAT 1 cut(s) 3127
CseI GACGC 2 cut(s) 3343, 3806
CsiI ACCWGGT 2 cut(s) 2065, 3977
Csp6I GTAC 8 cut(s) 452, 636, 2222, 2576, 3115, 3688, 3835, 4048
CspAI ACCGGT 1 cut(s) 2805
CviQI GTAC 8 cut(s) 452, 636, 2222, 2576, 3115, 3688, 3835, 4048
EaeI YGGCCR 2 cut(s) 190, 3952
EagI CGGCCG 1 cut(s) 190
Eam1104I CTCTTC 8 cut(s) 615, 1404, 1588, 1683, 3070, 3261, 3321, 4257
EarI CTCTTC 8 cut(s) 615, 1404, 1588, 1683, 3070, 3261, 3321, 4257
EciI GGCGGA 4 cut(s) 136, 158, 187, 307
Ecl136II GAGCTC 2 cut(s) 307, 1755
EclXI CGGCCG 1 cut(s) 190
Eco130I CCWWGG 9 cut(s) 228, 417, 537, 689, 711, 1242, 2003, 2478, 3260
Eco24I GRGCYC 2 cut(s) 309, 1757
Eco31I GGTCTC 2 cut(s) 2238, 2930
Eco32I GATATC 1 cut(s) 3045
Eco47I GGWCC 3 cut(s) 1588, 2513, 3539
Eco52I CGGCCG 1 cut(s) 190
Eco53kI GAGCTC 2 cut(s) 307, 1755
Eco57I CTGAAG 5 cut(s) 1011, 1385, 1856, 2751, 3264
Eco88I CYCGRG 2 cut(s) 73, 462
EcoICRI GAGCTC 2 cut(s) 307, 1755
EcoNI CCTNNNNNAGG 3 cut(s) 3941, 4070, 4232
EcoRI GAATTC 1 cut(s) 4040
EcoRII CCWGG 9 cut(s) 376, 774, 1701, 2065, 2755, 3094, 3243, 3949, 3977
EcoRV GATATC 1 cut(s) 3045
EcoT14I CCWWGG 9 cut(s) 228, 417, 537, 689, 711, 1242, 2003, 2478, 3260
EcoT22I ATGCAT 1 cut(s) 3817
EcoT38I GRGCYC 2 cut(s) 309, 1757
ErhI CCWWGG 9 cut(s) 228, 417, 537, 689, 711, 1242, 2003, 2478, 3260
Esp3I CGTCTC 1 cut(s) 585
FalI AAGNNNNNCTT 2 cut(s) 2088, 2120
FaqI GGGAC 3 cut(s) 191, 3226, 4115
FauI CCCGC 4 cut(s) 140, 1299, 2847, 4092
FauNDI CATATG 3 cut(s) 340, 1465, 3869
FbaI TGATCA 3 cut(s) 295, 1411, 2866
FblI GTMKAC 2 cut(s) 213, 261
FriOI GRGCYC 2 cut(s) 309, 1757
GlaI GCGC 6 cut(s) 729, 1110, 2102, 2441, 2728, 3146
GsaI CCCAGC 4 cut(s) 91, 1386, 2563, 4214
GsuI CTGGAG 5 cut(s) 122, 1685, 1796, 2214, 2778
HaeIII GGCC 7 cut(s) 78, 129, 192, 242, 257, 3222, 3954
HapII CCGG 8 cut(s) 126, 189, 518, 2471, 2806, 3161, 4045, 4170
HgaI GACGC 2 cut(s) 3343, 3806
HhaI GCGC 6 cut(s) 730, 1111, 2103, 2442, 2729, 3147
Hin1I GRCGYC 2 cut(s) 119, 3335
Hin6I GCGC 6 cut(s) 728, 1109, 2101, 2440, 2727, 3145
HinP1I GCGC 6 cut(s) 728, 1109, 2101, 2440, 2727, 3145
HincII GTYRAC 6 cut(s) 214, 575, 1478, 3333, 3370, 3522
HindII GTYRAC 6 cut(s) 214, 575, 1478, 3333, 3370, 3522
HindIII AAGCTT 1 cut(s) 2132
HpaII CCGG 8 cut(s) 126, 189, 518, 2471, 2806, 3161, 4045, 4170
HphI GGTGA 8 cut(s) 280, 1865, 1913, 2055, 2255, 2576, 3414, 3840
Hpy99I CGWCG 6 cut(s) 121, 124, 215, 218, 484, 3072
HpyCH4IV ACGT 9 cut(s) 119, 491, 638, 799, 1969, 3067, 3085, 3372, 3622
HpySE526I ACGT 9 cut(s) 119, 491, 638, 799, 1969, 3067, 3085, 3372, 3622
Hsp92I GRCGYC 2 cut(s) 119, 3335
HspAI GCGC 6 cut(s) 728, 1109, 2101, 2440, 2727, 3145
Kpn2I TCCGGA 1 cut(s) 3160
KpnI GGTACC 1 cut(s) 4051
Ksp22I TGATCA 3 cut(s) 295, 1411, 2866
LguI GCTCTTC 2 cut(s) 1588, 3070
LmnI GCTCC 8 cut(s) 985, 1001, 1011, 1225, 2816, 2896, 3952, 4151
MabI ACCWGGT 2 cut(s) 2065, 3977
MaeII ACGT 9 cut(s) 119, 491, 638, 799, 1969, 3067, 3085, 3372, 3622
MaeIII GTNAC 9 cut(s) 1393, 1420, 1982, 2038, 2197, 2407, 3207, 3452, 3623
MbiI CCGCTC 1 cut(s) 2230
MfeI CAATTG 1 cut(s) 3852
MflI RGATCY 3 cut(s) 1030, 1910, 2832
MhlI GDGCHC 3 cut(s) 309, 1757, 3313
MlsI TGGCCA 1 cut(s) 3954
MluNI TGGCCA 1 cut(s) 3954
MlyI GAGTC 7 cut(s) 227, 469, 939, 1692, 2046, 3735, 4097
MmeI TCCRAC 3 cut(s) 3597, 3998, 4323
Mox20I TGGCCA 1 cut(s) 3954
Mph1103I ATGCAT 1 cut(s) 3817
MroI TCCGGA 1 cut(s) 3160
MroXI GAANNNNTTC 1 cut(s) 3615
MscI TGGCCA 1 cut(s) 3954
MslI CAYNNNNRTG 2 cut(s) 954, 2715
Msp20I TGGCCA 1 cut(s) 3954
MspCI CTTAAG 1 cut(s) 3448
MspI CCGG 8 cut(s) 126, 189, 518, 2471, 2806, 3161, 4045, 4170
MunI CAATTG 1 cut(s) 3852
Mva1269I GAATGC 2 cut(s) 3180, 3443
MvaI CCWGG 9 cut(s) 378, 776, 1703, 2067, 2757, 3096, 3245, 3951, 3979
MvnI CGCG 1 cut(s) 730
NciI CCSGG 1 cut(s) 519
NcoI CCATGG 2 cut(s) 417, 2478
NdeI CATATG 3 cut(s) 340, 1465, 3869
NheI GCTAGC 1 cut(s) 3962
NmeAIII GCCGAG 3 cut(s) 72, 175, 1462
NmuCI GTSAC 4 cut(s) 1393, 2038, 2407, 3623
NsiI ATGCAT 1 cut(s) 3817
NspI RCATGY 1 cut(s) 3115
NspV TTCGAA 1 cut(s) 3172
PaeR7I CTCGAG 1 cut(s) 462
PagI TCATGA 1 cut(s) 2611
PciSI GCTCTTC 2 cut(s) 1588, 3070
PcsI WCGNNNNNNNCGW 2 cut(s) 488, 575
PctI GAATGC 2 cut(s) 3180, 3443
PdmI GAANNNNTTC 1 cut(s) 3615
PflFI GACNNNGTC 3 cut(s) 121, 478, 2036
PinAI ACCGGT 1 cut(s) 2805
PleI GAGTC 7 cut(s) 226, 469, 939, 1692, 2045, 3735, 4097
PpsI GAGTC 7 cut(s) 226, 469, 939, 1692, 2045, 3735, 4097
Ppu21I YACGTR 3 cut(s) 639, 800, 3623
PsiI TTATAA 1 cut(s) 3003
Psp124BI GAGCTC 2 cut(s) 309, 1757
Psp6I CCWGG 9 cut(s) 376, 774, 1701, 2065, 2755, 3094, 3243, 3949, 3977
PspFI CCCAGC 4 cut(s) 87, 1382, 2559, 4210
PspGI CCWGG 9 cut(s) 376, 774, 1701, 2065, 2755, 3094, 3243, 3949, 3977
PspPI GGNCC 6 cut(s) 76, 128, 1588, 2513, 3220, 3539
PstI CTGCAG 2 cut(s) 1060, 2389
PstNI CAGNNNCTG 2 cut(s) 2825, 3902
PsuI RGATCY 3 cut(s) 1030, 1910, 2832
PsyI GACNNNGTC 3 cut(s) 121, 478, 2036
RsaI GTAC 8 cut(s) 453, 637, 2223, 2577, 3116, 3689, 3836, 4049
RsaNI GTAC 8 cut(s) 452, 636, 2222, 2576, 3115, 3688, 3835, 4048
RseI CAYNNNNRTG 2 cut(s) 954, 2715
SacI GAGCTC 2 cut(s) 309, 1757
SalI GTCGAC 1 cut(s) 212
SapI GCTCTTC 2 cut(s) 1588, 3070
Sau96I GGNCC 6 cut(s) 76, 128, 1588, 2513, 3220, 3539
SchI GAGTC 7 cut(s) 227, 469, 939, 1692, 2046, 3735, 4097
SduI GDGCHC 3 cut(s) 309, 1757, 3313
SexAI ACCWGGT 2 cut(s) 2065, 3977
SfcI CTRYAG 5 cut(s) 876, 1056, 1148, 2385, 2629
Sfr274I CTCGAG 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 3172
SgrDI CGTCGACG 1 cut(s) 212
SinI GGWCC 3 cut(s) 1588, 2513, 3539
SlaI CTCGAG 1 cut(s) 462
SmiMI CAYNNNNRTG 2 cut(s) 954, 2715
SmlI CTYRAG 5 cut(s) 251, 462, 806, 2423, 3448
SmoI CTYRAG 5 cut(s) 251, 462, 806, 2423, 3448
SpeI ACTAGT 2 cut(s) 914, 3119
SspI AATATT 2 cut(s) 2929, 3408
SstI GAGCTC 2 cut(s) 309, 1757
StyI CCWWGG 9 cut(s) 228, 417, 537, 689, 711, 1242, 2003, 2478, 3260
TaiI ACGT 9 cut(s) 122, 494, 641, 802, 1972, 3070, 3088, 3375, 3625
TaqII GACCGA 2 cut(s) 1605, 2513
TatI WGTACW 6 cut(s) 451, 2221, 2575, 3114, 3687, 3834
TauI GCSGC 3 cut(s) 25, 174, 203
TscAI CASTG 9 cut(s) 1186, 1345, 1985, 2047, 2412, 3522, 3687, 3729, 3907
TseFI GTSAC 4 cut(s) 1393, 2038, 2407, 3623
Tsp45I GTSAC 4 cut(s) 1393, 2038, 2407, 3623
TspGWI ACGGA 4 cut(s) 468, 987, 988, 4232
TspRI CASTG 9 cut(s) 1186, 1345, 1985, 2047, 2412, 3522, 3687, 3729, 3907
Tth111I GACNNNGTC 3 cut(s) 121, 478, 2036
Vha464I CTTAAG 1 cut(s) 3448
VpaK11BI GGWCC 3 cut(s) 1588, 2513, 3539
XagI CCTNNNNNAGG 3 cut(s) 3941, 4070, 4232
XapI RAATTY 9 cut(s) 857, 1189, 1720, 1799, 2168, 2588, 2650, 2769, 4040
XbaI TCTAGA 1 cut(s) 3793
XceI RCATGY 1 cut(s) 3115
XcmI CCANNNNNNNNNTGG 1 cut(s) 1967
XhoI CTCGAG 1 cut(s) 462
XmiI GTMKAC 2 cut(s) 213, 261
XmnI GAANNNNTTC 1 cut(s) 3615
ZraI GACGTC 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 3817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.