AT1G58230

Domain of unknown function (DUF4800)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002031
Physical Location & Seq
Forward (+)
21566057 .. 21579159
13103 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G58230.3

Sequence Viewer

Length: 7815 bp
ATGGAATCTGACTCTGTCGCAGGATCGGCTTCAAGTTCTGATGCAGCTGCGTCTCATTTGCCAAGTGTGTCTGTCCTTTTAGATGCTCTTGAGCAGTCTGCATCTACACCTAGGATCGCAAATGGTCTTTCACAGAGGCTGTTACGTTCGTTACAGTTCTCAAATGAAAATAAACTTTCTTTTAAGGCGTTGAATGGAGCTTGTCGTGTGCTCAGATTGGCTTGTATTCAAGCTAAAGAAAGTAGGAAGTCCGGGTGTGTTAGCCCTTTAGTAGAAAGTAGTGACTGTGGAGTTATAGTTGATTCACCTCATAAGAAGTCTGATTCGAGTCATACAGAGGATTGTTGGTTTGAGTGCGTGGAATCTTGTGTGGGGATTTTCACTGAGTTTTTCTCGTCCACGAATGATGCCAAAGTATATGTTTTGCGAAGCTCCGTTTGTGTTGATTGTCTTTTTGAGCTGTTTTGGGAAAAAGCCGTTAGAAACAATGTGATGAAACTCATTATTGATCTCATGAAGATTAGGCCTTTGTGTGAGGAAGACAAAAGCGCAAAATTGCAAGTGTGTTCCAAATATCTAGAAACTTTCACTCAAGTGAAAGAAAGAGAAAATGATTCTGTTGACTTATCTGTTGATCTATTGGCGGGTATGAGGGATTTGATCAAGTCTGATTCGAGGTATTACCAAGCCCTATTCCGTGAGGGAGAGTGTTTCTTACACATTGTCTCTCTCTTAAATGGTAATCTCGATGAAGCAAATGGAGAGAAGTTGGTTTTAAATGTCCTTCAAACTCTAACTTCCCTGCTTGCAAATAATGATACGTCAAAGTTTGCATTTAAAGCCCTTGCTGGCAAGGGTTATCAGACACTTCAAAGCTTATTGTTGGATTTCTTCCAATGGAAACCTACCCAAAGGCTATTAGACGCATTACTTGATATGCTTGTTGATGGAAAATTTGATGATAAAGGCAGTGCCCTTATCAAGAATGAAGATGTTATCATACTTTACTTAAATGTACTTCAGAAGAGCAGCGAATCTTTGCAGTGTTATGGGCTAAATTTGTTTCAGCAACTCCTTAGGGATTCCATATCGAATCGTGCTTCATGTGTTCGGGCAGGAATGCTTCACCTTCTTCTTGATTGGTTTTCTCTGGAAAATGATGATAGTGTTATCTTGAAAATAACACAGCTAACTCAGACAATTGGTGGTCACAGTATTTCTGGGAAGGATATACGTAAGATCTTTGCTCTTCTTCGGAGTGAAAGAGTGGGCAATCAACAGAGATACCGCTCACTGTTATTGGCTTGTTTGTTGTCAATGCTGAATGAGAAAGGACCAACAGGCTTTTTTGACATGAATGGGGTTGAATCTGGGATTGTAATCAGAACACCTGTTCAGTGGCCTGCAAACAAGGGGTTTTCCTTTTGCTGTTGGCTTAGGGTAGAAAGCTTTCCTGGAGATGGCAAAATGGGTATCTTCAGTTTTATGTCAAAAAATGGAAAGGGTTGCTTTGCTGCTCTTGGGAAGGATGGACTATCTTATGTGTCTCTGAATCTGAAACGGCAATGTGTAAATGTGCATACCAATTTGGTCTGTAAAAAATGGCATTTTATTTGTGTATCTCATAGTATTGGTAGAGCATTCTGGGGTGGAAGCCTCTTGAGGTGTTATGTTAATGGGGATCTTGTGTCATCCGAAAGATGCAGCTATCCCAAAGTTACTGATGTACTTACCAGCTGCTTAATTGGTACAAGAATCACGTTGCCACATATCCAAGATAATGATGGCCTGGAATCGATCCGAGATGTGTTCCCATTTTTTGGTCAGATTGGCCCTGTTTACTTGTTCAATGATTCCTTGTCTTCCGAGCAAGTTCAGGCCATTTATTCGCTAGGACCAAGCTATATGTATGCATTCTTGGAGAATGAGATGACATGTCCCTTTTCTGATAATCCATTTCCTAGTGGCATTCTTGATGGAAAAGATGGGCTTGCATCTAAGGTTTCCTTTGGACTGAATGCACAGGCGAGCGATGGTAGGAGATTGTTTAATGTGTCTCGGGTGTCAGATCATCTACAAGAAAGGCTCGCATTTGAGGCCGATATTATGGTTGGTACTCAGCTATGCTCGCGGCGCTTGCTTCAGCAGATAATTTACTGTGTCGGAGGGATATCTGTTTTCTTTCCTTTGATAACACAATCTGATAGATGTGAAAGTGAAACACTGAACGAGGAAACTTCAGCTATGCCTACAAAAGAGCGTATGACAGCAGAAGTTATTGAACTGATTGCTTCTGTCTTGGATGAAAATCCAGCAAATCAGCAACAGATGCATCTTCTCGCTGGATTCCCCATACTAGGATTTTTATTACAGTCTATTCAACCGAAGCAACTCAATCTAGAGACACTTTCGTCTTTGAAACACCTCTTCAACGTTATTTCAAGCTCTGGGTTTGCTGAGCAGCTTGTGGAGGATGCTATATCTAGCATTTTCCTTAATCCTCACATTTGGCTCCATGCGGCCTATAATGTACAGCGTGAACTTTACATGTTTCTCATCCAACAGCTAGATAATGATCCACGACTTCTCGGAAGCCTTTGTCGACTCCCCAGAGTTATTGACATTGTATGGAACTTTTATTGGGAATCTGAAAGATACTGTAAAGGAAGCAAGCCTCTTATGCATCCAACAAGGACTATTGCAGAAAGACCAAGTAGAGATGAAATTCACAAAATTCGTCTCCTTTTGCTGAGCCTAGGTGAAATGAGTCTGAGGCATAATATCTCATCAGGGGACGTAAAAGCTTTGACAGCATTTTTTGAGACATGCCAGGATGTAGCATGTATCGAAGATGTTCTGCATATGGTTATCCGTGCCATTTCTCAAACATCGGTCCTTGTTTCGTTTCTTGAACAAGTCAATCTTATCGGTGGATGTCACATTTTCGTCGATCTTCTCCAGAGGGATTACGAGCCTATAAGGTTACTCAGCTTGCAGTTCCTTGGAAGGCTTCTTTATGATGTACCTTCTGAGAGAAAAGGACCTAAGTTTTTCAATCTTGCTGTTGGAAGAACTAAGTCTCTATCACAAGGGCATAAGAAAATTGGTGCTAGAACGCAACCAATTTTTTTGGCCATGTCTGATAGATTGTTCCAGTATCCTCAGACAGATAACTTGCGTGCAACCTTGTTTGACGTTCTTCTTGGTGGGGCCAGCCCCAAACAGGTTTTGCAGAAACACAACCAGGTTGATAAGCACAGAAGCAAACCAAGCAACTCTCACTTTTTCCTTCCACAAATTTTTGTTTTTATTTTCGAATTCCTCTCGGGCTGCAAGGATGGATTAGCACGAATGAAGATCATCAGTGACATACTCGATCTTCTTGATTCAAATCCTATGAATGTTGAAGCTTTGATGGAATTTGGATGGAGTGCGTGGTTAACAGCATCAATGAAACTTGATGTGATAAAAGACTACAGATCTGAATTGCTTAATCATGATGACTTGGCATTAAATGAGCAACATTTTGTCAGGGGTCTCTTTTGTGTTGTTCTCTGCCACTATATTTTATCTGTGAAGGGAGGTTGGCAGCAGCTAGAGGAAACTGTGAATTTCATACTCTTGCAGAGTGAGCATAATGATGTCCCGTACCGGTCTTTTCTCCGTGATCTCTATGAAGACTTGATTCAGCGTTTGGTGGAACTCTCATCTGAAGATAATATTTTCCTTTCGCACCCATGTCGCGACAATGTGCTGTATCTCTTGCGGCTCGTTGATGAGATGCTTGTCCGTGAATTTGGCAGTAGGCTCCTGTTTCCAGCAATTAGCACTGATTTTTCAGAGGATTTATTACAACTTGGAAACCGTGAGGACCCCACTTTGGGATTAGATGAGAGTTTCCAACGATTTTTGACTGAAGAAATCTCTAGAAACACAGAATGTCAGCAGTCTTGCACCACGGTAACTGAACTTATGACCAATGAAAGATGGTGGAATTTGTATGACAATCTGTGGAAGATAATATGTGATATTAATGGAAGGGGTCCGGTAAAGATGTCTCCCAAATCGTTAGCTACTGGTCCCTCTATTGGCCAAAGAGCAAGAGGTTTAGTAGAATCATTGAATGTTCCAGCTGCTGAAATGGCCGCAGTTGTTGTGTCAGGAGGAATTGGCAGTGCATTGAGTGGAAAAATGAACAAAAACGTTGATAAAGCAATGCTCCTAAGGGGGGAGAAATGCCCACGAATCGTGTTTAGGCTTGTCACCCTTTATCTGTGCATGTCTTCACTGGAAAAAGCCACTAGATGTGTACAGCAGGTGACCTCACTCTTGCCTTCCTTCTTAGCTGCTGATGATGAACAAAGCAAGAGCAGACTGCATCTCTTTATTGGGTGTTTGCTTTATGTGAGATCCCAGTATGGAAAGCTAGACGACGGTGCTCGTTTTCATGTCATATCGCACCTGATACGTGAAACTGTAAGTTGCGGAAAATCCATCCTTGCCACCAGTGGTATGAATAAAGATGACAGCTCTGATTCTGGTGGAATCTTCAAAGAAATGGGCTCTATCCAGAATCTTATACACAAAGACCGTGTGCTTGCTGCGGTCACTGATGAAACAACATACATGAAAACATTGATATCTGATCGTACTCGTCAAGTACAAGCCCTTGGTGAAAGGAATAATGAAACATTATCTATAGAATGCAACAGCAAGAAAGCTTTTGATGGTGAGCTGCAAAACGTTTTAAAAACTGTTGTTACATGGGATGAAAATAGACGAGTTTCAGTGCAATTAAGCCATGAAGAGCAACAACAAAATGTTACTGAGAAATGGATTCACATGCTGCGATCTTTGATGGATGAGAGGGGTCCTTGGTCTGCAACTCCTTTTCCTAATAACATTTTAAACCGCTGGAAACTTGACAGGACAGAAGATTCTTGGAGGCGTAGACCAAAGTTGAGACGAAATTATCATTTTGACGAAAGGCTGTGTCATCCACCTTCTACTTCCACTGCCACTGAAAATGAGACTTCAAACGTTATTAATGAAAGCAAGTCTGGTGTGATACATATACCTGAACAGATGAAGAAATTTTTACTTAAAGGCATTCGCAGGATAACCGATGAGGGTGGCTCAGATTCCTGTGAGAATGACAGTAGTCAAGCCGAGCAAAGTTTCATGGATACTTCTGCAGATATCCAGTTCTCTGAGCTTGTGAGAACCAGCAGTGGTCTCAAGGATGTCGTACAGGATAAAGTAGATGCATCTTCTCTAGAAGTTGGTACCAGCGAGGTTTTGACATCAGTTCCTTGTGTTCTCGTAACTCCGAAGAGGAAACTGGCTGGTTGGTTGGCAGTTATGAAAAATGTTTTACATTTTTCTGGGGAGTTTTTGGTGGAAGGAACTGGTGGATCAGCTGTCTTCAAGAACTTTAGCACTTCAAAGGGTAGTGATGTAACAAAGGCTGAAAATAAACAGAACCTTGTAAAATGGTCTTCGCCTTATGATAGTGAGACGTTTCTAGATTTGGAATCAGGAAACAAAAATAAGAAACCCTTAAAAAAGGTTAAGCGCCACAGAAGATGGAAAATAGGGAAGGTGAAAAGTGTTCATTGGACGCGGTATTTGCTTCAGTATACAGCTCTTGAGATTTTCTTCCAGGAATCTGTACCCCCAGTCTTTCTCAATTTTGCATCTCAAAAGAATGCAAAGGAGGTCGGGATGTTAATAGTTTCTACCAGGAATGAATTCCTGTTTCCAAAGAATGTTCCTAGGGACAGGACTGCAATGATTTCGTTTGTTGACAGAAGAATAGCTATGGAGATGGCAGAAACAGCCAGAGACAGATGGAGAAGGAGAGAGATTACAAATTTTGAATACTTGATGATTCTCAACACCCTTGCAGGCAGATCTTACAATGATTTGACTCAGTATCCTGTTTTCCCTTGGGTGGTGGCTGACTACTCATCAGAGACTCTTGATTTTAGCAAAGCATCTACCTTTCGTGATCTTTCTAAACCGGTTGGAGCTTTAGATACAAGAAGGTTTGAGATTTTTGAGGACAGATATCACAGCTTCTCTGATCCTGATATACCTAGTTTCTATTACGGGTCCCACTATTCAAGTATGGGAAGTGTGTTGTATTACCTTTTGAGGTTAGAGCCGTTTACATCCCTTCATCGTAGTCTGCAGGGTGGTAAATTTGATCACGCAGATCGTCTTTTCCAAAGTGTTGAGGGGTCCTTTCGAAATTGCCTGTCCAATACAAGCGATGTAAAAGAATTGATCCCTGAGTTCTTCTACATGCCTGAATTTCTGGTTAATTCTAACTCGTACCATCTTGGAGTAAAACAAGATGGTGAACCTCTTGGTGAAGTTTGTCTACCTCCATGGGCTAAGGGTTCTCCAGAGATGTTCATTGCTCGTAATAGAGAAGCCCTAGAGAGCGAGTATGTTAGTTCACATCTCCATGATTGGATTGACTTGATATTTGGACACAAACAGCGTGGCAAACCAGCTGTGGAGGCTGCAAATATCTTTTATTACCTTACATATGAAGGTGCTGTCGACGTAGAAAACATGGAAGATCAACTGCAAATATCAGCAATTGAAGATCAGATCGCTAATTTTGGTCAAACTCCAATCCAAATATTCCGGAAGAAACATCCGAGAAGGGGGCCACCTATTCCAATTGCACATCCGTTGTACTTTGCGCCTGCTTCCATTAATTTGTCTTCTATTCTTCCCGCTACAACTCATTCCCCATCAGCAGTTCTATATGTTGGTGTAGTGGATTCAAATATAGTACTCGTAAACCAGGGTCTAACTTTGTCAGTCAAAATTTGGTTGACAACTCAACTACATTCTGGCGGAAATTTTACATTTTCTAGCGCTCAGGATCCATTCTTTGGAGTTGGTTCGGATGTTCTTTCTCCCCGTAACATTGGAAGTCCCCTAGCAGACAACGTTGAACTTGGATCACAGTGCTTTGCTGCAATGCAAATGCCACTGGAAAACTTTCTGGTTTCATGTGGGAACTGGGAGAACAGTTTTCATGTCATATCCTTGACTGATGGTCGGGTAGTCCAAAGCATCCGACATCATAAAGATGTTGTAAGCTGTGTTGCAGTGACTGCTGATTCGACTATCCTGGCAACTGGTAGCTATGATACGACTGTGATGGTGTGGGACATTCTCCGCATGAGAACACCTGAAAAGAGGGTTAGAAATACCCACGCAGAAGTTCTGCGGAAAGATATCGTCATAGCCGATGCTCCTTCTCATATTCTTTGTGGCCATGATGATATAATAACCTGTCTATATGTCAGCACTGATCTTGATATAGTCATCAGTGGGTCAAAAGATGGAACCTGTGTATTCCACACATTGCGGGAAGGGAGATACATAAGATCTTTAAAACATCCTTCGGGCAGTGCAGTCTCAAAGCTTGCTGCATCGCACCATGGACGGATTGTTCTATACGGTGATGATGATCTTAGTCTGCACCTCTACTCCATAAACGGAAAACATCTGGCCAGCTCAGAGTCAAATGGACGAATCAACTGTCTTGAACTTAGTAAGTGTGGAGAGTTCTTGGTCAGTGCCGGTGACCAAGGACAAATAATTGTGCGGTCTATGAACACCCTTGAAGTAGTAAAACGGTATAACGGCGCAGGGAAAATAATCACCTCTCTGACAGTAACTCAGGAAGAGTGTTTCTTAGCAGGGACAAAAGATGGAGCCCTTCTTGTTTATTCGATCGAGAATCCTCAACATCGGAAACCGAGCCCAATCTGGAGCATAAAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Beach DUF4704 DUF4800 Laminin_G_3 PH_BEACH WD40

Protein Analysis

2604

Amino Acids

292.78

Weight (kDa)

6.32

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neurobeachin PF20425 19 - 235 1e-122 Neurobeachin alpha solenoid region
Laminin_G_3 PF13385 251 - 420 2e-07 Concanavalin A-like lectin/glucanases superfamily
DUF4704 PF15787 491 - 1001 3.9e-167 Neurobeachin/BDCP, DUF4704 alpha solenoid region
DUF4800 PF16057 1363 - 1456 5e-07 Domain of unknown function (DUF4800)
PH_BEACH PF14844 1568 - 1704 6.6e-11 PH domain associated with Beige/BEACH
Beach PF02138 1744 - 2020 1.2e-120 Beige/BEACH domain
NBCH_WD40 PF20426 2041 - 2389 3.2e-198 Neurobeachin beta propeller domain
Beta-prop_EML_2 PF23414 2118 - 2266 8.7e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 2132 - 2196 9.3e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 2133 - 2306 7.3e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 2136 - 2195 4.8e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 2138 - 2196 1.2e-07 THOC3 beta-propeller domain
WD40 PF00400 2155 - 2192 2.7e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 2155 - 2312 6.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 2156 - 2310 2.2e-08 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 2222 - 2383 5.7e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 2223 - 2383 8.5e-12 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 2223 - 2370 9.7e-10 WDR5 beta-propeller domain
WDR55 PF24796 2227 - 2382 7.9e-09 WDR55
Beta-prop_WDR3_1st PF25173 2257 - 2384 1.3e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4306
AasI GACNNNNNNGTC 1 cut(s) 2410
Acc36I ACCTGC 1 cut(s) 4306
Acc65I GGTACC 1 cut(s) 5285
AccB1I GGYRCC 1 cut(s) 5285
AccB7I CCANNNNNTGG 2 cut(s) 1820, 6911
AccBSI CCGCTC 1 cut(s) 1290
AccI GTMKAC 5 cut(s) 2602, 4951, 5639, 6385, 6570
AccII CGCG 3 cut(s) 2131, 3744, 5623
AccIII TCCGGA 1 cut(s) 6657
AclI AACGTT 5 cut(s) 2433, 4203, 4743, 5040, 6969
AcoI YGGCCR 5 cut(s) 3132, 4090, 4143, 7297, 7536
AcuI CTGAAG 7 cut(s) 1004, 1462, 2126, 2223, 3732, 3936, 5618
AfeI AGCGCT 1 cut(s) 6895
AflIII ACRYGT 2 cut(s) 1934, 2547
AgeI ACCGGT 2 cut(s) 3651, 6022
AhdI GACNNNNNGTC 1 cut(s) 7077
AjnI CCWGG 8 cut(s) 1453, 1788, 2829, 3243, 5661, 5741, 6819, 7152
AleI CACNNNNGTG 1 cut(s) 593
AloI GAACNNNNNNTCC 2 cut(s) 7461, 7493
Alw21I GWGCWC 2 cut(s) 213, 4441
AlwNI CAGNNNCTG 3 cut(s) 139, 4136, 7136
Ama87I CYCGRG 2 cut(s) 2058, 3325
Aor13HI TCCGGA 1 cut(s) 6657
Aor51HI AGCGCT 1 cut(s) 6895
ArsI GACNNNNNNTTYG 8 cut(s) 5611, 5643, 6476, 6508, 7126, 7158, 7552, 7584
AseI ATTAAT 3 cut(s) 4032, 5046, 6729
AsiGI ACCGGT 2 cut(s) 3651, 6022
Asp700I GAANNNNTTC 4 cut(s) 1449, 2853, 5750, 7020
Asp718I GGTACC 1 cut(s) 5285
AspA2I CCTAGG 3 cut(s) 110, 2755, 5774
AspLEI GCGC 6 cut(s) 551, 2136, 5577, 6718, 6896, 7676
AsuC2I CCSGG 1 cut(s) 253
AsuII TTCGAA 2 cut(s) 3315, 6250
AvaI CYCGRG 2 cut(s) 2058, 3325
AvrII CCTAGG 3 cut(s) 110, 2755, 5774
AxyI CCTNAGG 2 cut(s) 1076, 4223
BaeGI GKGCMC 1 cut(s) 976
BaeI ACNNNNGTAYC 2 cut(s) 7164, 7197
BalI TGGCCA 4 cut(s) 3134, 4092, 7299, 7538
BamHI GGATCC 1 cut(s) 6901
BanI GGYRCC 1 cut(s) 5285
BanII GRGCYC 3 cut(s) 4565, 7747, 7793
BarI GAAGNNNNNNTAC 2 cut(s) 6369, 6401
BbsI GAAGAC 7 cut(s) 546, 1854, 3684, 4275, 5416, 5490, 6729
Bbv12I GWGCWC 2 cut(s) 213, 4441
BceAI ACGGC 4 cut(s) 461, 1577, 6151, 7687
BcgI CGANNNNNNTGC 4 cut(s) 3722, 3756, 5778, 5812
BciT130I CCWGG 8 cut(s) 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
BciVI GTATCC 3 cut(s) 3168, 5179, 5946
BclI TGATCA 2 cut(s) 660, 6208
BcnI CCSGG 1 cut(s) 253
BfmI CTRYAG 4 cut(s) 3475, 4698, 5193, 6191
BfoI RGCGCY 3 cut(s) 2137, 5578, 6897
BfuAI ACCTGC 1 cut(s) 4306
BfuI GTATCC 3 cut(s) 3168, 5179, 5946
BglII AGATCT 4 cut(s) 1239, 3479, 5912, 7412
BlnI CCTAGG 3 cut(s) 110, 2755, 5774
BlpI GCTNAGC 2 cut(s) 2457, 2750
BmcAI AGTACT 1 cut(s) 6810
Bme1390I CCNGG 9 cut(s) 253, 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
BmeRI GACNNNNNGTC 1 cut(s) 7077
BmeT110I CYCGRG 2 cut(s) 2058, 3325
BmrFI CCNGG 9 cut(s) 253, 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
BmrI ACTGGG 3 cut(s) 4408, 5672, 7051
BmuI ACTGGG 3 cut(s) 4408, 5672, 7051
BoxI GACNNNNGTC 1 cut(s) 5160
BpiI GAAGAC 7 cut(s) 546, 1854, 3684, 4275, 5416, 5490, 6729
BplI GAGNNNNNCTC 4 cut(s) 377, 409, 5120, 5152
BpmI CTGGAG 3 cut(s) 1476, 2942, 6393
Bpu10I CCTNAGC 3 cut(s) 1436, 6399, 6897
Bpu1102I GCTNAGC 2 cut(s) 2457, 2750
Bpu14I TTCGAA 2 cut(s) 3315, 6250
BpuEI CTTGAG 5 cut(s) 110, 576, 1681, 5222, 5669
BpuMI CCSGG 1 cut(s) 253
Bsa29I ATCGAT 1 cut(s) 1796
BsaAI YACGTR 2 cut(s) 1235, 4469
BsaBI GATNNNNATC 5 cut(s) 1379, 2307, 2574, 3390, 7494
BsaI GGTCTC 2 cut(s) 3542, 5240
BsaWI WCCGGW 4 cut(s) 3651, 4045, 6022, 6657
BsaXI ACNNNNNCTCC 4 cut(s) 282, 312, 7499, 7529
Bse118I RCCGGY 3 cut(s) 3651, 6022, 7607
Bse21I CCTNAGG 2 cut(s) 1076, 4223
Bse3DI GCAATG 6 cut(s) 1571, 4221, 5796, 6420, 7005, 7388
Bse8I GATNNNNATC 5 cut(s) 1379, 2307, 2574, 3390, 7494
BseAI TCCGGA 1 cut(s) 6657
BseBI CCWGG 8 cut(s) 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
BseCI ATCGAT 1 cut(s) 1796
BseJI GATNNNNATC 5 cut(s) 1379, 2307, 2574, 3390, 7494
BseMI GCAATG 6 cut(s) 1571, 4221, 5796, 6420, 7005, 7388
BseSI GKGCMC 1 cut(s) 976
BsgI GTGCAG 2 cut(s) 7458, 7490
Bsh1236I CGCG 3 cut(s) 2131, 3744, 5623
Bsh1285I CGRYCG 1 cut(s) 7764
BshNI GGYRCC 1 cut(s) 5285
BshTI ACCGGT 2 cut(s) 3651, 6022
BshVI ATCGAT 1 cut(s) 1796
BsiEI CGRYCG 1 cut(s) 7764
BsiHKAI GWGCWC 2 cut(s) 213, 4441
BsiHKCI CYCGRG 2 cut(s) 2058, 3325
BsiSI CCGG 6 cut(s) 252, 3652, 4046, 6023, 6658, 7608
BslFI GGGAC 9 cut(s) 1923, 2807, 3629, 4065, 5792, 6100, 6939, 7205, 7744
BsmBI CGTCTC 4 cut(s) 57, 2744, 4957, 5510
BsmFI GGGAC 9 cut(s) 1923, 2807, 3629, 4065, 5792, 6100, 6939, 7205, 7744
BsmI GAATGC 8 cut(s) 1125, 1640, 1913, 1968, 2023, 4709, 5109, 5713
Bso31I GGTCTC 2 cut(s) 3542, 5240
BsoBI CYCGRG 2 cut(s) 2058, 3325
Bsp119I TTCGAA 2 cut(s) 3315, 6250
Bsp1286I GDGCHC 6 cut(s) 213, 976, 4441, 4565, 7747, 7793
Bsp13I TCCGGA 1 cut(s) 6657
Bsp1407I TGTACA 2 cut(s) 2530, 4309
Bsp1720I GCTNAGC 2 cut(s) 2457, 2750
Bsp19I CCATGG 2 cut(s) 6392, 7465
Bsp68I TCGCGA 1 cut(s) 3744
BspDI ATCGAT 1 cut(s) 1796
BspEI TCCGGA 1 cut(s) 6657
BspFNI CGCG 3 cut(s) 2131, 3744, 5623
BspHI TCATGA 2 cut(s) 513, 3496
BspMAI CTGCAG 2 cut(s) 5197, 6195
BspMI ACCTGC 1 cut(s) 4306
BspQI GCTCTTC 3 cut(s) 1019, 1254, 4800
BspT104I TTCGAA 2 cut(s) 3315, 6250
BspT107I GGYRCC 1 cut(s) 5285
BspTNI GGTCTC 2 cut(s) 3542, 5240
BsrBI CCGCTC 1 cut(s) 1290
BsrDI GCAATG 6 cut(s) 1571, 4221, 5796, 6420, 7005, 7388
BsrFI RCCGGY 3 cut(s) 3651, 6022, 7607
BsrGI TGTACA 2 cut(s) 2530, 4309
BssAI RCCGGY 3 cut(s) 3651, 6022, 7607
BssNAI GTATAC 1 cut(s) 5640
Bst1107I GTATAC 1 cut(s) 5640
Bst2UI CCWGG 8 cut(s) 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
Bst6I CTCTTC 6 cut(s) 1019, 1254, 2432, 4800, 5327, 7707
BstAPI GCANNNNNTGC 2 cut(s) 2329, 7136
BstAUI TGTACA 2 cut(s) 2530, 4309
BstBAI YACGTR 2 cut(s) 1235, 4469
BstBI TTCGAA 2 cut(s) 3315, 6250
BstDSI CCRYGG 3 cut(s) 3957, 6392, 7465
BstEII GGTNACC 2 cut(s) 4318, 7610
BstFNI CGCG 3 cut(s) 2131, 3744, 5623
BstH2I RGCGCY 3 cut(s) 2137, 5578, 6897
BstHHI GCGC 6 cut(s) 551, 2136, 5577, 6718, 6896, 7676
BstMCI CGRYCG 1 cut(s) 7764
BstNI CCWGG 8 cut(s) 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
BstNSI RCATGY 7 cut(s) 1938, 2551, 2829, 2844, 4282, 4846, 6310
BstPAI GACNNNNGTC 1 cut(s) 5160
BstPI GGTNACC 2 cut(s) 4318, 7610
BstSCI CCNGG 9 cut(s) 251, 1453, 1788, 2829, 3243, 5661, 5741, 6819, 7152
BstSFI CTRYAG 4 cut(s) 3475, 4698, 5193, 6191
BstSLI GKGCMC 1 cut(s) 976
BstSNI TACGTA 1 cut(s) 1235
BstUI CGCG 3 cut(s) 2131, 3744, 5623
BstV2I GAAGAC 7 cut(s) 546, 1854, 3684, 4275, 5416, 5490, 6729
BstX2I RGATCY 7 cut(s) 1239, 1681, 3479, 4409, 5912, 6901, 7412
BstYI RGATCY 7 cut(s) 1239, 1681, 3479, 4409, 5912, 6901, 7412
BstZ17I GTATAC 1 cut(s) 5640
Bsu15I ATCGAT 1 cut(s) 1796
Bsu36I CCTNAGG 2 cut(s) 1076, 4223
BsuI GTATCC 3 cut(s) 3168, 5179, 5946
BsuTUI ATCGAT 1 cut(s) 1796
BtgI CCRYGG 3 cut(s) 3957, 6392, 7465
BtgZI GCGATG 3 cut(s) 2046, 6288, 7443
BtsI GCAGTG 7 cut(s) 976, 1049, 4180, 5013, 5236, 7137, 7441
BtuMI TCGCGA 1 cut(s) 3744
BveI ACCTGC 1 cut(s) 4306
CaiI CAGNNNCTG 3 cut(s) 139, 4136, 7136
CciI TCATGA 2 cut(s) 513, 3496
CfoI GCGC 6 cut(s) 551, 2136, 5577, 6718, 6896, 7676
Cfr10I RCCGGY 3 cut(s) 3651, 6022, 7607
ClaI ATCGAT 1 cut(s) 1796
CseI GACGC 3 cut(s) 39, 932, 5629
CsiI ACCWGGT 1 cut(s) 3243
CspAI ACCGGT 2 cut(s) 3651, 6022
CspCI CAANNNNNGTGG 2 cut(s) 6490, 6525
DraI TTTAAA 5 cut(s) 777, 838, 4749, 4908, 7419
DrdI GACNNNNNNGTC 1 cut(s) 2410
DriI GACNNNNNGTC 1 cut(s) 7077
DseDI GACNNNNNNGTC 1 cut(s) 2410
EaeI YGGCCR 5 cut(s) 3132, 4090, 4143, 7297, 7536
Eam1104I CTCTTC 6 cut(s) 1019, 1254, 2432, 4800, 5327, 7707
Eam1105I GACNNNNNGTC 1 cut(s) 7077
EarI CTCTTC 6 cut(s) 1019, 1254, 2432, 4800, 5327, 7707
EciI GGCGGA 1 cut(s) 6888
Eco105I TACGTA 1 cut(s) 1235
Eco147I AGGCCT 1 cut(s) 526
Eco24I GRGCYC 3 cut(s) 4565, 7747, 7793
Eco31I GGTCTC 2 cut(s) 3542, 5240
Eco32I GATATC 5 cut(s) 2172, 4641, 5200, 6071, 7261
Eco47III AGCGCT 1 cut(s) 6895
Eco57I CTGAAG 7 cut(s) 1004, 1462, 2126, 2223, 3732, 3936, 5618
Eco81I CCTNAGG 2 cut(s) 1076, 4223
Eco88I CYCGRG 2 cut(s) 2058, 3325
Eco91I GGTNACC 2 cut(s) 4318, 7610
EcoO109I RGGNCCY 5 cut(s) 3041, 3871, 4871, 6114, 6243
EcoO65I GGTNACC 2 cut(s) 4318, 7610
EcoRI GAATTC 2 cut(s) 3317, 5750
EcoRII CCWGG 8 cut(s) 1453, 1788, 2829, 3243, 5661, 5741, 6819, 7152
EcoRV GATATC 5 cut(s) 2172, 4641, 5200, 6071, 7261
EcoT22I ATGCAT 4 cut(s) 1915, 2334, 2685, 5269
EcoT38I GRGCYC 3 cut(s) 4565, 7747, 7793
Esp3I CGTCTC 4 cut(s) 57, 2744, 4957, 5510
FaqI GGGAC 9 cut(s) 1923, 2807, 3629, 4065, 5792, 6100, 6939, 7205, 7744
FauI CCCGC 3 cut(s) 637, 6757, 7386
FauNDI CATATG 2 cut(s) 2862, 6556
FbaI TGATCA 2 cut(s) 660, 6208
FblI GTMKAC 5 cut(s) 2602, 4951, 5639, 6385, 6570
FriOI GRGCYC 3 cut(s) 4565, 7747, 7793
GlaI GCGC 6 cut(s) 550, 2135, 5576, 6717, 6895, 7675
GsuI CTGGAG 3 cut(s) 1476, 2942, 6393
HaeII RGCGCY 3 cut(s) 2137, 5578, 6897
HapII CCGG 6 cut(s) 252, 3652, 4046, 6023, 6658, 7608
HgaI GACGC 3 cut(s) 39, 932, 5629
HhaI GCGC 6 cut(s) 551, 2136, 5577, 6718, 6896, 7676
Hin6I GCGC 6 cut(s) 549, 2134, 5575, 6716, 6894, 7674
HinP1I GCGC 6 cut(s) 549, 2134, 5575, 6716, 6894, 7674
HincII GTYRAC 6 cut(s) 622, 2603, 3441, 5806, 6571, 6852
HindII GTYRAC 6 cut(s) 622, 2603, 3441, 5806, 6571, 6852
HindIII AAGCTT 6 cut(s) 874, 1447, 2802, 3408, 4719, 7448
HpaI GTTAAC 1 cut(s) 3441
HpaII CCGG 6 cut(s) 252, 3652, 4046, 6023, 6658, 7608
Hpy99I CGWCG 3 cut(s) 2951, 4436, 6575
HspAI GCGC 6 cut(s) 549, 2134, 5575, 6716, 6894, 7674
KflI GGGWCCC 1 cut(s) 6114
Kpn2I TCCGGA 1 cut(s) 6657
KpnI GGTACC 1 cut(s) 5289
Ksp22I TGATCA 2 cut(s) 660, 6208
KspAI GTTAAC 1 cut(s) 3441
LguI GCTCTTC 3 cut(s) 1019, 1254, 4800
LmnI GCTCC 9 cut(s) 197, 437, 2517, 3813, 4224, 6029, 7282, 7742, 7800
MabI ACCWGGT 1 cut(s) 3243
MbiI CCGCTC 1 cut(s) 1290
MfeI CAATTG 3 cut(s) 1200, 6609, 6693
MflI RGATCY 7 cut(s) 1239, 1681, 3479, 4409, 5912, 6901, 7412
MhlI GDGCHC 6 cut(s) 213, 976, 4441, 4565, 7747, 7793
MlsI TGGCCA 4 cut(s) 3134, 4092, 7299, 7538
MluNI TGGCCA 4 cut(s) 3134, 4092, 7299, 7538
MlyI GAGTC 7 cut(s) 5, 337, 2598, 2776, 5923, 5971, 7556
MmeI TCCRAC 8 cut(s) 864, 2143, 2584, 2711, 3046, 3925, 6007, 7123
Mox20I TGGCCA 4 cut(s) 3134, 4092, 7299, 7538
Mph1103I ATGCAT 4 cut(s) 1915, 2334, 2685, 5269
MroI TCCGGA 1 cut(s) 6657
MroXI GAANNNNTTC 4 cut(s) 1449, 2853, 5750, 7020
MscI TGGCCA 4 cut(s) 3134, 4092, 7299, 7538
MslI CAYNNNNRTG 4 cut(s) 593, 3639, 6471, 7110
Msp20I TGGCCA 4 cut(s) 3134, 4092, 7299, 7538
MspA1I CMGCKG 6 cut(s) 47, 1737, 4133, 4914, 5420, 6521
MspI CCGG 6 cut(s) 252, 3652, 4046, 6023, 6658, 7608
MspR9I CCNGG 9 cut(s) 253, 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
MunI CAATTG 3 cut(s) 1200, 6609, 6693
Mva1269I GAATGC 8 cut(s) 1125, 1640, 1913, 1968, 2023, 4709, 5109, 5713
MvaI CCWGG 8 cut(s) 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
MvnI CGCG 3 cut(s) 2131, 3744, 5623
NciI CCSGG 1 cut(s) 253
NcoI CCATGG 2 cut(s) 6392, 7465
NdeI CATATG 2 cut(s) 2862, 6556
NmeAIII GCCGAG 1 cut(s) 5194
NmuCI GTSAC 9 cut(s) 281, 1208, 2936, 3365, 4261, 4318, 4606, 7132, 7610
NruI TCGCGA 1 cut(s) 3744
NsiI ATGCAT 4 cut(s) 1915, 2334, 2685, 5269
NspI RCATGY 7 cut(s) 1938, 2551, 2829, 2844, 4282, 4846, 6310
NspV TTCGAA 2 cut(s) 3315, 6250
OliI CACNNNNGTG 1 cut(s) 593
PagI TCATGA 2 cut(s) 513, 3496
PaqCI CACCTGC 1 cut(s) 4306
PceI AGGCCT 1 cut(s) 526
PciI ACATGT 2 cut(s) 1934, 2547
PciSI GCTCTTC 3 cut(s) 1019, 1254, 4800
PcsI WCGNNNNNNNCGW 1 cut(s) 7269
PctI GAATGC 8 cut(s) 1125, 1640, 1913, 1968, 2023, 4709, 5109, 5713
PdmI GAANNNNTTC 4 cut(s) 1449, 2853, 5750, 7020
PflFI GACNNNGTC 1 cut(s) 14
PflMI CCANNNNNTGG 2 cut(s) 1820, 6911
PfoI TCCNGGA 2 cut(s) 1453, 5661
PinAI ACCGGT 2 cut(s) 3651, 6022
Ple19I CGATCG 1 cut(s) 7764
PleI GAGTC 7 cut(s) 5, 336, 2598, 2775, 5923, 5971, 7555
PpsI GAGTC 7 cut(s) 5, 336, 2598, 2775, 5923, 5971, 7555
Ppu21I YACGTR 2 cut(s) 1235, 4469
PpuMI RGGWCCY 5 cut(s) 3041, 3871, 4871, 6114, 6243
PscI ACATGT 2 cut(s) 1934, 2547
PshAI GACNNNNGTC 1 cut(s) 5160
PshBI ATTAAT 3 cut(s) 4032, 5046, 6729
Psp1406I AACGTT 5 cut(s) 2433, 4203, 4743, 5040, 6969
Psp5II RGGWCCY 5 cut(s) 3041, 3871, 4871, 6114, 6243
Psp6I CCWGG 8 cut(s) 1453, 1788, 2829, 3243, 5661, 5741, 6819, 7152
PspEI GGTNACC 2 cut(s) 4318, 7610
PspGI CCWGG 8 cut(s) 1453, 1788, 2829, 3243, 5661, 5741, 6819, 7152
PspPPI RGGWCCY 5 cut(s) 3041, 3871, 4871, 6114, 6243
PstI CTGCAG 2 cut(s) 5197, 6195
PstNI CAGNNNCTG 3 cut(s) 139, 4136, 7136
PsuI RGATCY 7 cut(s) 1239, 1681, 3479, 4409, 5912, 6901, 7412
PsyI GACNNNGTC 1 cut(s) 14
PvuI CGATCG 1 cut(s) 7764
PvuII CAGCTG 5 cut(s) 47, 1737, 4133, 5420, 6521
RruI TCGCGA 1 cut(s) 3744
RseI CAYNNNNRTG 4 cut(s) 593, 3639, 6471, 7110
SalI GTCGAC 2 cut(s) 2601, 6569
SapI GCTCTTC 3 cut(s) 1019, 1254, 4800
ScaI AGTACT 1 cut(s) 6810
SchI GAGTC 7 cut(s) 5, 337, 2598, 2776, 5923, 5971, 7556
ScrFI CCNGG 9 cut(s) 253, 1455, 1790, 2831, 3245, 5663, 5743, 6821, 7154
SduI GDGCHC 6 cut(s) 213, 976, 4441, 4565, 7747, 7793
SexAI ACCWGGT 1 cut(s) 3243
SfcI CTRYAG 4 cut(s) 3475, 4698, 5193, 6191
SfuI TTCGAA 2 cut(s) 3315, 6250
SmiMI CAYNNNNRTG 4 cut(s) 593, 3639, 6471, 7110
SmlI CTYRAG 5 cut(s) 89, 591, 1660, 5237, 5648
SmoI CTYRAG 5 cut(s) 89, 591, 1660, 5237, 5648
SnaBI TACGTA 1 cut(s) 1235
SseBI AGGCCT 1 cut(s) 526
SspI AATATT 2 cut(s) 3721, 6654
StuI AGGCCT 1 cut(s) 526
StyD4I CCNGG 9 cut(s) 251, 1453, 1788, 2829, 3243, 5661, 5741, 6819, 7152
TaqII GACCGA 1 cut(s) 2881
TatI WGTACW 7 cut(s) 1015, 1726, 2530, 4309, 4660, 6708, 6808
TauI GCSGC 4 cut(s) 2134, 2522, 3769, 4148
TseFI GTSAC 9 cut(s) 281, 1208, 2936, 3365, 4261, 4318, 4606, 7132, 7610
Tsp45I GTSAC 9 cut(s) 281, 1208, 2936, 3365, 4261, 4318, 4606, 7132, 7610
TspGWI ACGGA 8 cut(s) 424, 686, 2861, 3653, 3779, 6693, 7486, 7539
Tth111I GACNNNGTC 1 cut(s) 14
Van91I CCANNNNNTGG 2 cut(s) 1820, 6911
VspI ATTAAT 3 cut(s) 4032, 5046, 6729
XbaI TCTAGA 5 cut(s) 577, 2398, 3926, 5275, 5524
XceI RCATGY 7 cut(s) 1938, 2551, 2829, 2844, 4282, 4846, 6310
XcmI CCANNNNNNNNNTGG 1 cut(s) 1781
XmaJI CCTAGG 3 cut(s) 110, 2755, 5774
XmiI GTMKAC 5 cut(s) 2602, 4951, 5639, 6385, 6570
XmnI GAANNNNTTC 4 cut(s) 1449, 2853, 5750, 7020
ZrmI AGTACT 1 cut(s) 6810
Zsp2I ATGCAT 4 cut(s) 1915, 2334, 2685, 5269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.