Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003881

Physical Location & Seq

Genomic Coordinates Reverse (-)

24508482 .. 24510742

Gene Structure 2261 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G65880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGGATGATTTGGCATTATGTGAAGCAAACAATGTTCCTCTAACCCCCATGACGTTCTTGAAGAGAGCTTCAGAGTGTTATCCAAATCGAACTTCAATAATCTACGGAAAAACTCGTTTCACTTGGCCTCAGACCTATGACCGTTGTTGTCGTCTAGCCGCTTCTCTAATCTCTCTTAACATCTCCAAGAACGATGTGGTATCAGTTATGGCTCCAAACACACCCGCCTTGTATGAAATGCACTTTGCCGTTCCCATGGCTGGAGCTGTACTTAACCCTATCAACACTCGTCTAGACGCAACATCCATTGCCGCAATCCTCCGCCACGCCAAGCCCAAGATCTTATTCCTAGACCGCAGTTTTGAGGCCTTGGCTAGAGAAAGCCTCCATTTATTATCATCTGAAGACTCAAACCTAAACTTGCCGGTCATATTTATCCACGAGAACGATTTTCCTAAAAGGGCTTCATTCGAGGAGTTAGACTACGAGTGTCTCATCCAGAGGGGAGAGCCTACGCCCTCGATGGTGGCACGCATGTTCCGTATTCAAGACGAGCATGATCCAATCTCCTTAAACTACACATCGGGTACCACTGCCGACCCAAAAGGTGTTGTGATTAGCCACCGAGGAGCATATTTGTGCACATTAAGCGCGATTATTGGTTGGGAAATGGGGACCTGCCCTGTCTACCTTTGGACTCTGCCTATGTTTCATTGCAATGGATGGACGTTTACATGGGGAACCGCGGCGCGTGGGGGTACCAGTGTGTGTATGAGGCACGTGACTGCCCCGGAGATCTATAAAAACATAGAAATGCATAACGTGACACATATGTGTTGTGTTCCTACGGTTTTCAACATTCTTCTGAAAGGAAATTCACTTGACCTGTCACCTAGATCTGGACCGGTCCATGTGCTTACCGGAGGTTCACCGCCTCCCGCTGCTCTTGTCAAGAAAGTTCAACGGTTGGGGTTTCAAGTGATGCATGCCTATGGGCAGACCGAGGCCACTGGTCCAATTTTGTTTTGTGAGTGGCAAGATGAGTGGAATAGATTACCAGAGAATCAACAGATGGAATTAAAAGCCAGGCAAGGGATAAGCATCTTAGGCCTAGCTGACGTTGACGTGAAAAACAAGGAAACGCAAAAGAGTGCTCCGCGCGATGGAAAGACAATGGGAGAAATCCTCATTAAAGGAAGTAGCATAATGAAAGGGTATCTAAAAAATCCCAAAGCTACATTTGAGGCATTTAAACACGGATGGCTCAACACAGGAGATGTAGGTGTGATTCACCCTGATGGGCACGTCGAGATCAAAGATCGGTCAAAAGACATAATCATATCGGGAGGCGAAAACATTAGTAGTGTTGAGGTCGAGAATGTTCTTTATAAGTACCCAAAAGTCCTTGAGACTGCAGTTGTGGCCATGCCTCACCCTACGTGGGGTGAAACCCCGTGTGCGTTTGTTGTTCTAGAAAAGAGTGAGACTACTATTAAAGAAGATCGTGTTGATAAATTTCAGACCAGAGAGAGAAATCTGATTGAGTATTGCCGTGAAAATCTGCCACATTTTATGTGTCCGAGAAAAGTGGTGTTTTTGGAAGAACTGCCCAAAAACGGGAATGGAAAGATCCTTAAGCCTAAGCTAAGAGACATTGCTAAAGGTTTGGTTGTTGAGGATGAGATCAATGTTATAGCTAAAGAAGTTAAACGGCCGGTTGGACATTTTATTTCGCGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0016143 GO:0016144 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018858 GO:0019605 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046459 GO:0047760 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

580

Amino Acids

64.94

Weight (kDa)

7.56

Isoelectric Point (pI)

37.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	21 - 407	7.4e-83	AMP-binding enzyme
AMP-binding_C	PF13193	457 - 543	1.3e-22	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65880 AT1G65890 AT1G66120
fragaria_vesca	FvH4_1g27440 FvH4_1g27450
malus_domestica	MD09G1280400.v1.1 MD17G1287600.v1.1
prunus_persica	Prupe.3G013700_v2.0.a1
pyrus_communis	pycom09g18850 pycom17g27060
rosa_chinensis	RchiOBHm_Chr3g0488561 RchiOBHm_Chr3g0488571
rosa_laevigata	RLG00000022942 RLG00000022943
rosa_multiflora	Rmu_sc0004402.1_g000006 Rmu_sc0004402.1_g000007 Rmu_sc0005363.1_g000024 Rmu_sc0005363.1_g000025
rosa_roxburghii	Rroxscaffold_6G00394130 Rroxscaffold_6G00394140
rosa_rugosa	Rorug03G0239300 Rorug03G0239400
rosa_samantha	Rh3AG288300 Rh3AG288400 Rh3BG325000 Rh3BG325100 Rh3CG322200 Rh3CG322300 Rh3DG321000 Rh3DG321100 Rh4AG012700 Rh7BG359000
rosa_wichuraiana	Rw3G025510 Rw3G025520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1389
Acc36I	ACCTGC	1 cut(s)	686
Acc65I	GGTACC	2 cut(s)	587, 758
AccB1I	GGYRCC	2 cut(s)	587, 758
AccI	GTMKAC	1 cut(s)	687
AccII	CGCG	6 cut(s)	653, 746, 751, 1159, 1161, 1735
AclWI	GGATC	2 cut(s)	554, 1624
AcoI	YGGCCR	2 cut(s)	1422, 1712
AcsI	RAATTY	2 cut(s)	874, 1514
AcuI	CTGAAG	2 cut(s)	54, 423
AcvI	CACGTG	1 cut(s)	781
AfaI	GTAC	4 cut(s)	270, 589, 760, 1394
AfiI	CCNNNNNNNGG	8 cut(s)	260, 899, 1092, 1163, 1441, 1442, 1616, 1617
AflII	CTTAAG	1 cut(s)	1634
AgeI	ACCGGT	1 cut(s)	904
AgsI	TTSAA	6 cut(s)	61, 96, 548, 856, 962, 977
AjiI	CACGTC	2 cut(s)	1126, 1306
AjnI	CCWGG	1 cut(s)	1085
AjuI	GAANNNNNNNTTGG	4 cut(s)	76, 108, 329, 361
AleI	CACNNNNGTG	1 cut(s)	832
AluBI	AGCT	6 cut(s)	68, 266, 1115, 1235, 1645, 1697
AluI	AGCT	6 cut(s)	68, 266, 1115, 1235, 1645, 1697
Alw21I	GWGCWC	2 cut(s)	644, 1156
Alw26I	GTCTC	4 cut(s)	497, 1403, 1478, 1644
Alw44I	GTGCAC	1 cut(s)	640
AlwI	GGATC	2 cut(s)	554, 1624
AoxI	GGCC	6 cut(s)	125, 366, 1005, 1108, 1422, 1712
ApaLI	GTGCAC	1 cut(s)	640
ApeKI	GCWGC	1 cut(s)	941
ApoI	RAATTY	2 cut(s)	874, 1514
ArsI	GACNNNNNNTTYG	2 cut(s)	344, 376
AsiGI	ACCGGT	1 cut(s)	904
Asp718I	GGTACC	2 cut(s)	587, 758
AspLEI	GCGC	3 cut(s)	653, 751, 1161
AspS9I	GGNCC	4 cut(s)	675, 902, 907, 1013
AsuC2I	CCSGG	1 cut(s)	791
AsuHPI	GGTGA	5 cut(s)	882, 921, 1283, 1424, 1457
AvaII	GGWCC	4 cut(s)	675, 902, 907, 1013
BaeGI	GKGCMC	2 cut(s)	644, 1305
BaeI	ACNNNNGTAYC	2 cut(s)	750, 783
BalI	TGGCCA	1 cut(s)	1424
BanI	GGYRCC	2 cut(s)	587, 758
BauI	CACGAG	1 cut(s)	440
BbrPI	CACGTG	1 cut(s)	781
BbsI	GAAGAC	1 cut(s)	411
Bbv12I	GWGCWC	2 cut(s)	644, 1156
BbvI	GCAGC	1 cut(s)	928
BccI	CCATC	6 cut(s)	517, 717, 1066, 1157, 1254, 1292
BceAI	ACGGC	3 cut(s)	233, 1536, 1727
BciT130I	CCWGG	1 cut(s)	1087
BcnI	CCSGG	1 cut(s)	791
BcoDI	GTCTC	4 cut(s)	497, 1403, 1478, 1644
BfaI	CTAG	7 cut(s)	155, 293, 350, 375, 894, 1112, 1472
BfmI	CTRYAG	1 cut(s)	1413
BfrI	CTTAAG	1 cut(s)	1634
BfuAI	ACCTGC	1 cut(s)	686
BglII	AGATCT	3 cut(s)	339, 795, 896
BisI	GCNGC	5 cut(s)	159, 312, 747, 942, 1736
BlsI	GCNGC	5 cut(s)	160, 313, 748, 943, 1737
Bme1390I	CCNGG	2 cut(s)	791, 1087
Bme18I	GGWCC	4 cut(s)	675, 902, 907, 1013
BmgBI	CACGTC	2 cut(s)	1126, 1306
BmgT120I	GGNCC	4 cut(s)	675, 902, 907, 1013
BmiI	GGNNCC	5 cut(s)	213, 589, 676, 742, 760
BmrFI	CCNGG	2 cut(s)	791, 1087
BmsI	GCATC	2 cut(s)	972, 1110
BpiI	GAAGAC	1 cut(s)	411
BplI	GAGNNNNNCTC	4 cut(s)	369, 401, 1170, 1202
BpmI	CTGGAG	1 cut(s)	282
Bpu10I	CCTNAGC	1 cut(s)	1641
BpuEI	CTTGAG	1 cut(s)	1427
BpuMI	CCSGG	1 cut(s)	791
BsaAI	YACGTR	2 cut(s)	781, 1440
BsaBI	GATNNNNATC	1 cut(s)	1100
BsaJI	CCNNGG	6 cut(s)	255, 369, 625, 744, 789, 1002
BsaWI	WCCGGW	2 cut(s)	904, 920
Bsc4I	CCNNNNNNNGG	8 cut(s)	260, 899, 1092, 1163, 1441, 1442, 1616, 1617
Bse118I	RCCGGY	3 cut(s)	424, 904, 1714
Bse1I	ACTGG	2 cut(s)	762, 1015
Bse3DI	GCAATG	4 cut(s)	306, 712, 724, 1653
Bse8I	GATNNNNATC	1 cut(s)	1100
BseBI	CCWGG	1 cut(s)	1087
BseDI	CCNNGG	6 cut(s)	255, 369, 625, 744, 789, 1002
BseGI	GGATG	6 cut(s)	10, 302, 495, 728, 1265, 1684
BseJI	GATNNNNATC	1 cut(s)	1100
BseLI	CCNNNNNNNGG	8 cut(s)	260, 899, 1092, 1163, 1441, 1442, 1616, 1617
BseMI	GCAATG	4 cut(s)	306, 712, 724, 1653
BseMII	CTCAG	1 cut(s)	143
BseNI	ACTGG	2 cut(s)	762, 1015
BseRI	GAGGAG	2 cut(s)	488, 642
BseSI	GKGCMC	2 cut(s)	644, 1305
BseX3I	CGGCCG	1 cut(s)	1712
BseXI	GCAGC	1 cut(s)	928
Bsh1236I	CGCG	6 cut(s)	653, 746, 751, 1159, 1161, 1735
Bsh1285I	CGRYCG	1 cut(s)	1715
BshFI	GGCC	6 cut(s)	127, 368, 1007, 1110, 1424, 1714
BshNI	GGYRCC	2 cut(s)	587, 758
BshTI	ACCGGT	1 cut(s)	904
BsiEI	CGRYCG	1 cut(s)	1715
BsiHKAI	GWGCWC	2 cut(s)	644, 1156
BsiSI	CCGG	5 cut(s)	425, 791, 905, 921, 1715
BslFI	GGGAC	1 cut(s)	688
BslI	CCNNNNNNNGG	8 cut(s)	260, 899, 1092, 1163, 1441, 1442, 1616, 1617
BsmAI	GTCTC	4 cut(s)	497, 1403, 1478, 1644
BsmFI	GGGAC	1 cut(s)	688
BsnI	GGCC	6 cut(s)	127, 368, 1007, 1110, 1424, 1714
Bsp1286I	GDGCHC	3 cut(s)	644, 1156, 1305
Bsp143I	GATC	9 cut(s)	339, 559, 795, 896, 1311, 1318, 1501, 1629, 1683
Bsp19I	CCATGG	1 cut(s)	255
BspANI	GGCC	6 cut(s)	127, 368, 1007, 1110, 1424, 1714
BspCNI	CTCAG	1 cut(s)	142
BspFNI	CGCG	6 cut(s)	653, 746, 751, 1159, 1161, 1735
BspLI	GGNNCC	5 cut(s)	213, 589, 676, 742, 760
BspMAI	CTGCAG	1 cut(s)	1417
BspMI	ACCTGC	1 cut(s)	686
BspPI	GGATC	2 cut(s)	554, 1624
BspT107I	GGYRCC	2 cut(s)	587, 758
BspTI	CTTAAG	1 cut(s)	1634
BsrDI	GCAATG	4 cut(s)	306, 712, 724, 1653
BsrFI	RCCGGY	3 cut(s)	424, 904, 1714
BsrI	ACTGG	2 cut(s)	762, 1015
BssAI	RCCGGY	3 cut(s)	424, 904, 1714
BssECI	CCNNGG	6 cut(s)	255, 369, 625, 744, 789, 1002
BssMI	GATC	9 cut(s)	339, 559, 795, 896, 1311, 1318, 1501, 1629, 1683
BssSI	CACGAG	1 cut(s)	440
BssT1I	CCWWGG	2 cut(s)	255, 369
Bst2BI	CACGAG	1 cut(s)	440
Bst2UI	CCWGG	1 cut(s)	1087
Bst4CI	ACNGT	3 cut(s)	143, 850, 966
Bst6I	CTCTTC	1 cut(s)	56
BstAFI	CTTAAG	1 cut(s)	1634
BstBAI	YACGTR	2 cut(s)	781, 1440
BstC8I	GCNNGC	2 cut(s)	532, 987
BstDEI	CTNAG	4 cut(s)	129, 1105, 1641, 1646
BstDSI	CCRYGG	2 cut(s)	255, 744
BstF5I	GGATG	6 cut(s)	10, 302, 495, 728, 1265, 1684
BstFNI	CGCG	6 cut(s)	653, 746, 751, 1159, 1161, 1735
BstHHI	GCGC	3 cut(s)	653, 751, 1161
BstKTI	GATC	9 cut(s)	342, 562, 798, 899, 1314, 1321, 1504, 1632, 1686
BstMAI	GTCTC	4 cut(s)	497, 1403, 1478, 1644
BstMBI	GATC	9 cut(s)	339, 559, 795, 896, 1311, 1318, 1501, 1629, 1683
BstMCI	CGRYCG	1 cut(s)	1715
BstMWI	GCNNNNNNNGC	3 cut(s)	648, 1107, 1421
BstNI	CCWGG	1 cut(s)	1087
BstNSI	RCATGY	2 cut(s)	538, 989
BstSCI	CCNGG	2 cut(s)	789, 1085
BstSFI	CTRYAG	1 cut(s)	1413
BstSLI	GKGCMC	2 cut(s)	644, 1305
BstUI	CGCG	6 cut(s)	653, 746, 751, 1159, 1161, 1735
BstV1I	GCAGC	1 cut(s)	928
BstV2I	GAAGAC	1 cut(s)	411
BstX2I	RGATCY	4 cut(s)	339, 795, 896, 1629
BstYI	RGATCY	4 cut(s)	339, 795, 896, 1629
BstZI	CGGCCG	1 cut(s)	1712
BsuRI	GGCC	6 cut(s)	127, 368, 1007, 1110, 1424, 1714
BtgI	CCRYGG	2 cut(s)	255, 744
BtgZI	GCGATG	1 cut(s)	1176
BtrI	CACGTC	2 cut(s)	1126, 1306
BtsCI	GGATG	6 cut(s)	10, 302, 495, 728, 1265, 1684
BtsI	GCAGTG	1 cut(s)	591
BtsIMutI	CAGTG	3 cut(s)	591, 769, 1008
BveI	ACCTGC	1 cut(s)	686
Cac8I	GCNNGC	2 cut(s)	532, 987
CfoI	GCGC	3 cut(s)	653, 751, 1161
Cfr10I	RCCGGY	3 cut(s)	424, 904, 1714
Cfr13I	GGNCC	4 cut(s)	675, 902, 907, 1013
Cfr42I	CCGCGG	1 cut(s)	747
CseI	GACGC	1 cut(s)	305
Csp6I	GTAC	4 cut(s)	269, 588, 759, 1393
CspAI	ACCGGT	1 cut(s)	904
CspCI	CAANNNNNGTGG	2 cut(s)	1025, 1060
CviAII	CATG	8 cut(s)	49, 256, 535, 557, 735, 911, 986, 1426
CviQI	GTAC	4 cut(s)	269, 588, 759, 1393
DdeI	CTNAG	4 cut(s)	129, 1105, 1641, 1646
DpnI	GATC	9 cut(s)	341, 561, 797, 898, 1313, 1320, 1503, 1631, 1685
DpnII	GATC	9 cut(s)	339, 559, 795, 896, 1311, 1318, 1501, 1629, 1683
DraI	TTTAAA	1 cut(s)	1252
EaeI	YGGCCR	2 cut(s)	1422, 1712
EagI	CGGCCG	1 cut(s)	1712
Eam1104I	CTCTTC	1 cut(s)	56
EarI	CTCTTC	1 cut(s)	56
EciI	GGCGGA	1 cut(s)	311
EclXI	CGGCCG	1 cut(s)	1712
Eco130I	CCWWGG	2 cut(s)	255, 369
Eco147I	AGGCCT	2 cut(s)	368, 1110
Eco47I	GGWCC	4 cut(s)	675, 902, 907, 1013
Eco52I	CGGCCG	1 cut(s)	1712
Eco57I	CTGAAG	2 cut(s)	54, 423
Eco72I	CACGTG	1 cut(s)	781
EcoO109I	RGGNCCY	1 cut(s)	675
EcoRII	CCWGG	1 cut(s)	1085
EcoT14I	CCWWGG	2 cut(s)	255, 369
EcoT22I	ATGCAT	2 cut(s)	819, 987
ErhI	CCWWGG	2 cut(s)	255, 369
FaeI	CATG	8 cut(s)	52, 259, 538, 560, 738, 914, 989, 1429
FaqI	GGGAC	1 cut(s)	688
FatI	CATG	8 cut(s)	48, 255, 534, 556, 734, 910, 985, 1425
FauI	CCCGC	2 cut(s)	232, 946
FauNDI	CATATG	1 cut(s)	831
FblI	GTMKAC	1 cut(s)	687
Fnu4HI	GCNGC	5 cut(s)	159, 312, 747, 942, 1736
FokI	GGATG	6 cut(s)	17, 289, 482, 735, 1272, 1691
Fsp4HI	GCNGC	5 cut(s)	159, 312, 747, 942, 1736
FspBI	CTAG	7 cut(s)	155, 293, 350, 375, 894, 1112, 1472
GlaI	GCGC	3 cut(s)	652, 750, 1160
GluI	GCNGC	5 cut(s)	159, 312, 747, 942, 1736
GsuI	CTGGAG	1 cut(s)	282
HaeIII	GGCC	6 cut(s)	127, 368, 1007, 1110, 1424, 1714
HapII	CCGG	5 cut(s)	425, 791, 905, 921, 1715
HgaI	GACGC	1 cut(s)	305
HhaI	GCGC	3 cut(s)	653, 751, 1161
Hin1II	CATG	8 cut(s)	52, 259, 538, 560, 738, 914, 989, 1429
Hin6I	GCGC	3 cut(s)	651, 749, 1159
HinP1I	GCGC	3 cut(s)	651, 749, 1159
HincII	GTYRAC	1 cut(s)	1123
HindII	GTYRAC	1 cut(s)	1123
HinfI	GANTC	4 cut(s)	407, 697, 1063, 1288
HpaII	CCGG	5 cut(s)	425, 791, 905, 921, 1715
HphI	GGTGA	5 cut(s)	882, 921, 1283, 1424, 1457
Hpy166II	GTNNAC	5 cut(s)	642, 688, 732, 929, 1123
Hpy188I	TCNGA	7 cut(s)	73, 132, 403, 867, 1521, 1539, 1581
Hpy8I	GTNNAC	5 cut(s)	642, 688, 732, 929, 1123
Hpy99I	CGWCG	1 cut(s)	1310
HpyCH4III	ACNGT	3 cut(s)	143, 850, 966
HpyCH4IV	ACGT	8 cut(s)	53, 728, 780, 822, 1119, 1125, 1305, 1439
HpyCH4V	TGCA	6 cut(s)	241, 642, 717, 817, 985, 1415
HpyF10VI	GCNNNNNNNGC	3 cut(s)	648, 1107, 1421
HpyF3I	CTNAG	4 cut(s)	129, 1105, 1641, 1646
HpySE526I	ACGT	8 cut(s)	53, 728, 780, 822, 1119, 1125, 1305, 1439
Hsp92II	CATG	8 cut(s)	52, 259, 538, 560, 738, 914, 989, 1429
HspAI	GCGC	3 cut(s)	651, 749, 1159
KpnI	GGTACC	2 cut(s)	591, 762
KspI	CCGCGG	1 cut(s)	747
Kzo9I	GATC	9 cut(s)	339, 559, 795, 896, 1311, 1318, 1501, 1629, 1683
LmnI	GCTCC	4 cut(s)	217, 263, 629, 1159
Lsp1109I	GCAGC	1 cut(s)	928
LweI	GCATC	2 cut(s)	972, 1110
MaeI	CTAG	7 cut(s)	155, 293, 350, 375, 894, 1112, 1472
MaeII	ACGT	8 cut(s)	53, 728, 780, 822, 1119, 1125, 1305, 1439
MaeIII	GTNAC	3 cut(s)	781, 823, 888
MalI	GATC	9 cut(s)	341, 561, 797, 898, 1313, 1320, 1503, 1631, 1685
MboI	GATC	9 cut(s)	339, 559, 795, 896, 1311, 1318, 1501, 1629, 1683
MboII	GAAGA	5 cut(s)	73, 416, 854, 1511, 1613
MflI	RGATCY	4 cut(s)	339, 795, 896, 1629
MhlI	GDGCHC	3 cut(s)	644, 1156, 1305
MlsI	TGGCCA	1 cut(s)	1424
MluCI	AATT	4 cut(s)	874, 1017, 1076, 1514
MluNI	TGGCCA	1 cut(s)	1424
MlyI	GAGTC	2 cut(s)	401, 691
MmeI	TCCRAC	1 cut(s)	1699
Mox20I	TGGCCA	1 cut(s)	1424
Mph1103I	ATGCAT	2 cut(s)	819, 987
MscI	TGGCCA	1 cut(s)	1424
MslI	CAYNNNNRTG	6 cut(s)	637, 717, 812, 832, 990, 1296
Msp20I	TGGCCA	1 cut(s)	1424
MspA1I	CMGCKG	2 cut(s)	746, 941
MspCI	CTTAAG	1 cut(s)	1634
MspI	CCGG	5 cut(s)	425, 791, 905, 921, 1715
MspR9I	CCNGG	2 cut(s)	791, 1087
MvaI	CCWGG	1 cut(s)	1087
MvnI	CGCG	6 cut(s)	653, 746, 751, 1159, 1161, 1735
MwoI	GCNNNNNNNGC	3 cut(s)	648, 1107, 1421
NciI	CCSGG	1 cut(s)	791
NcoI	CCATGG	1 cut(s)	255
NdeI	CATATG	1 cut(s)	831
NdeII	GATC	9 cut(s)	339, 559, 795, 896, 1311, 1318, 1501, 1629, 1683
NlaIII	CATG	8 cut(s)	52, 259, 538, 560, 738, 914, 989, 1429
NlaIV	GGNNCC	5 cut(s)	213, 589, 676, 742, 760
NmuCI	GTSAC	3 cut(s)	781, 823, 888
NsiI	ATGCAT	2 cut(s)	819, 987
NspI	RCATGY	2 cut(s)	538, 989
OliI	CACNNNNGTG	1 cut(s)	832
PaeI	GCATGC	1 cut(s)	989
PceI	AGGCCT	2 cut(s)	368, 1110
PcsI	WCGNNNNNNNCGW	1 cut(s)	538
PfeI	GAWTC	2 cut(s)	1063, 1288
PinAI	ACCGGT	1 cut(s)	904
PkrI	GCNGC	5 cut(s)	160, 313, 748, 943, 1737
PleI	GAGTC	2 cut(s)	401, 691
PmaCI	CACGTG	1 cut(s)	781
PmlI	CACGTG	1 cut(s)	781
PpsI	GAGTC	2 cut(s)	401, 691
Ppu21I	YACGTR	2 cut(s)	781, 1440
PpuMI	RGGWCCY	1 cut(s)	675
PsiI	TTATAA	1 cut(s)	1389
Psp5II	RGGWCCY	1 cut(s)	675
Psp6I	CCWGG	1 cut(s)	1085
PspCI	CACGTG	1 cut(s)	781
PspGI	CCWGG	1 cut(s)	1085
PspN4I	GGNNCC	5 cut(s)	213, 589, 676, 742, 760
PspPI	GGNCC	4 cut(s)	675, 902, 907, 1013
PspPPI	RGGWCCY	1 cut(s)	675
PstI	CTGCAG	1 cut(s)	1417
PsuI	RGATCY	4 cut(s)	339, 795, 896, 1629
RsaI	GTAC	4 cut(s)	270, 589, 760, 1394
RsaNI	GTAC	4 cut(s)	269, 588, 759, 1393
RseI	CAYNNNNRTG	6 cut(s)	637, 717, 812, 832, 990, 1296
SacII	CCGCGG	1 cut(s)	747
SatI	GCNGC	5 cut(s)	159, 312, 747, 942, 1736
Sau3AI	GATC	9 cut(s)	339, 559, 795, 896, 1311, 1318, 1501, 1629, 1683
Sau96I	GGNCC	4 cut(s)	675, 902, 907, 1013
SchI	GAGTC	2 cut(s)	401, 691
ScrFI	CCNGG	2 cut(s)	791, 1087
SduI	GDGCHC	3 cut(s)	644, 1156, 1305
SfaNI	GCATC	2 cut(s)	972, 1110
SfcI	CTRYAG	1 cut(s)	1413
Sfr303I	CCGCGG	1 cut(s)	747
SgrBI	CCGCGG	1 cut(s)	747
SinI	GGWCC	4 cut(s)	675, 902, 907, 1013
SmiMI	CAYNNNNRTG	6 cut(s)	637, 717, 812, 832, 990, 1296
SmlI	CTYRAG	2 cut(s)	1406, 1634
SmoI	CTYRAG	2 cut(s)	1406, 1634
SphI	GCATGC	1 cut(s)	989
Sse9I	AATT	4 cut(s)	874, 1017, 1076, 1514
SseBI	AGGCCT	2 cut(s)	368, 1110
SspMI	CTAG	7 cut(s)	155, 293, 350, 375, 894, 1112, 1472
StuI	AGGCCT	2 cut(s)	368, 1110
StyD4I	CCNGG	2 cut(s)	789, 1085
StyI	CCWWGG	2 cut(s)	255, 369
TaaI	ACNGT	3 cut(s)	143, 850, 966
TaiI	ACGT	8 cut(s)	56, 731, 783, 825, 1122, 1128, 1308, 1442
TaqI	TCGA	5 cut(s)	88, 471, 521, 1308, 1374
TaqII	GACCGA	2 cut(s)	1016, 1311
TasI	AATT	4 cut(s)	874, 1017, 1076, 1514
TatI	WGTACW	1 cut(s)	268
TauI	GCSGC	4 cut(s)	161, 314, 749, 1738
TfiI	GAWTC	2 cut(s)	1063, 1288
TscAI	CASTG	3 cut(s)	598, 769, 1015
TseFI	GTSAC	3 cut(s)	781, 823, 888
TseI	GCWGC	1 cut(s)	941
Tsp45I	GTSAC	3 cut(s)	781, 823, 888
TspDTI	ATGAA	4 cut(s)	249, 456, 701, 1223
TspGWI	ACGGA	3 cut(s)	120, 530, 1272
TspRI	CASTG	3 cut(s)	598, 769, 1015
Vha464I	CTTAAG	1 cut(s)	1634
VneI	GTGCAC	1 cut(s)	640
VpaK11BI	GGWCC	4 cut(s)	675, 902, 907, 1013
XapI	RAATTY	2 cut(s)	874, 1514
XbaI	TCTAGA	2 cut(s)	292, 1471
XceI	RCATGY	2 cut(s)	538, 989
XcmI	CCANNNNNNNNNTGG	1 cut(s)	193
XmiI	GTMKAC	1 cut(s)	687
XspI	CTAG	7 cut(s)	155, 293, 350, 375, 894, 1112, 1472
Zsp2I	ATGCAT	2 cut(s)	819, 987

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.