Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0003881

Physical Location & Seq

Genomic Coordinates Reverse (-)

19132863 .. 19134900

Gene Structure 2038 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g27450.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1749 bp

ATGGGAACGGAGATGATGAATATGCTTCCCAAATGTGAAGCCAACTACGCTGCTCTCACCCCCACTACTTTCTTGAAGAGAGCTGCGTCTTTTTATGCTGACCGCACCTCCCTCATCTACGAAGGCACTCGCTTCACTTGGCGCCAGACCTACGACCGCTGCTCTCGTCTCGCCTCCTCTCTTGTTTGCCTCAACATTCTCAACCATGATGTGGTATCAGTCCTGGCTCCTAATGTTCCGGCAATGTACGAGATGCACTTTGCGGTGCCTATGGCCGGTGCGGTGCTCAACACCATCAACACCCGCCTCGATGCCAAGAACATTGCTTCCATTCTCAAACACTCAGGCGCCAAGGTCTTCTTTGTGGACTACCAGTTCGTCCCGGTGGCCAGTGAGGCTCTCAGAATCCTTATGAACACTTCTGCTGAGCCCTCCATGATGCCGTTAGTCATCGTGATCGACGACATCGACTCTCCCACCGGGGTTCGGTTGGGAGAACTCGAGTACGAGCAGCTCATTCACAAGGGCAATCCACGGTTCGTCCCCGTCGACGTCGCCGACGAGTGGGATCCAATTGCCTTGAACTACACTTCTGGCACAACTTCTGAACCAAAAGGTGTTGTGTACAGCCACAGAGGTGCTTACTTAAGCACTTTAAGTTTAATCCTGGGTTGGGAAATGGGGAGCGAGCCTGTTTATCTATGGACACTCCCTATGTTCCACTGCAACGGCTGGACCTTCACTTGGGGTGTGGCGGCACGCGGCGGAACCAACGTCTGCCTCCGCAACACCACCGCGTATGACATCTACCGCAACATCAAACGACACCAGGTCACCCACATGTGCTGTGCTCCCATCATCTTCAACATTCTCCTCGAGGCCAAACCCCACGAGCGCTGCGAGATCGTCTCCCCTATCCAAATACTTACCGGTGGAGCACCTCCCCCAGCTCCGTTGCTTGAGAAAATCGAGCCGTTGGGTTTCAAGGTGACACATGCATATGGCCTGACTGAAGCAACCGGACCGGCTTTGGTTTGCGAGTGGCAGGCCAAGTGGAACAAGCTGCCCAAGGAGGAGCAGTCGAATCTCAAAGCAAGGCAAGGAATAAGCATTTTGACTCTCGCTGACGTTGACGTGAAGGACTCGGATACAATGGAGAGTGTTCCTCACGACGGCAAAACCATGGGGGAGATTGTGCTGCGAGGGAGTAGCATCATGAAGGGTTACTTCAAGGACCCAAAGGCCACGAACAAAGCGTTCAAGAACGGCTGGTTCTTCACCGGCGACGTCGGTGTTGTCCACCCGGATGGTTATTTGGAAATTAAAGACAGGTCAAAAGACGTCATCATCTCTGGCGGTGAGAACATTAGTAGTGTGGAGTTGGAGAGCGTGCTTTACAGGCACCCGAACATTCTAGAAGCGGCGGCGGTGGCAATGCCTCACCCGCGGTGGGGTGAGACTCCTTGTGCTTTCGTGGCCCTTAGAAGCAACGACTCCAACGTGAACGTAAAGGAGTCTGATATCATCAATTACTGCCGGAAGAACTTGCCGCACTTTATGGTTCCGAAAAGGGTGGTGTTTGTGCCTGAACTGCCCAAGACTAACGGGAAGATTCTCAAGTTCAAGCTGAGGGAACGGGCTAAGGCCTTCGAACCCGTCGACGTATCGGAAAATCAGCAGCAAGCCGTCGTGAATTCTCACCCAAATTCTGGCTACAATGAGCAGATTCTGGCTTTGTCCCGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0016143 GO:0016144 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018858 GO:0019605 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046459 GO:0047760 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

583

Amino Acids

64.79

Weight (kDa)

6.77

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	26 - 410	3.6e-83	AMP-binding enzyme
AMP-binding_C	PF13193	460 - 535	2.2e-21	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65880 AT1G65890 AT1G66120
fragaria_vesca	FvH4_1g27440 FvH4_1g27450
malus_domestica	MD09G1280400.v1.1 MD17G1287600.v1.1
prunus_persica	Prupe.3G013700_v2.0.a1
pyrus_communis	pycom09g18850 pycom17g27060
rosa_chinensis	RchiOBHm_Chr3g0488561 RchiOBHm_Chr3g0488571
rosa_laevigata	RLG00000022942 RLG00000022943
rosa_multiflora	Rmu_sc0004402.1_g000006 Rmu_sc0004402.1_g000007 Rmu_sc0005363.1_g000024 Rmu_sc0005363.1_g000025
rosa_roxburghii	Rroxscaffold_6G00394130 Rroxscaffold_6G00394140
rosa_rugosa	Rorug03G0239300 Rorug03G0239400
rosa_samantha	Rh3AG288300 Rh3AG288400 Rh3BG325000 Rh3BG325100 Rh3CG322200 Rh3CG322300 Rh3DG321000 Rh3DG321100 Rh4AG012700 Rh7BG359000
rosa_wichuraiana	Rw3G025510 Rw3G025520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	3 cut(s)	555, 1290, 1344
AbsI	CCTCGAGG	1 cut(s)	875
AccB1I	GGYRCC	4 cut(s)	141, 265, 347, 1401
AccB7I	CCANNNNNTGG	2 cut(s)	211, 1709
AccI	GTMKAC	2 cut(s)	549, 1659
AccII	CGCG	3 cut(s)	762, 797, 1447
AclWI	GGATC	2 cut(s)	563, 576
AcoI	YGGCCR	2 cut(s)	273, 387
AcsI	RAATTY	2 cut(s)	1693, 1705
AcuI	CTGAAG	1 cut(s)	1032
AcyI	GRCGYC	5 cut(s)	142, 348, 552, 1287, 1341
AfaI	GTAC	3 cut(s)	248, 506, 626
AfeI	AGCGCT	1 cut(s)	896
AflII	CTTAAG	1 cut(s)	646
AflIII	ACRYGT	1 cut(s)	840
AgeI	ACCGGT	1 cut(s)	929
AgsI	TTSAA	7 cut(s)	76, 583, 865, 985, 1231, 1261, 1624
AhdI	GACNNNNNGTC	1 cut(s)	830
AjiI	CACGTC	1 cut(s)	1135
AjnI	CCWGG	3 cut(s)	222, 666, 828
AleI	CACNNNNGTG	1 cut(s)	636
AloI	GAACNNNNNNTCC	4 cut(s)	311, 343, 359, 391
AluBI	AGCT	5 cut(s)	83, 514, 950, 1063, 1627
AluI	AGCT	5 cut(s)	83, 514, 950, 1063, 1627
Alw21I	GWGCWC	3 cut(s)	288, 853, 940
Alw26I	GTCTC	3 cut(s)	173, 913, 1451
AlwI	GGATC	2 cut(s)	563, 576
AlwNI	CAGNNNCTG	1 cut(s)	1729
Ama87I	CYCGRG	2 cut(s)	500, 875
Aor51HI	AGCGCT	1 cut(s)	896
AoxI	GGCC	8 cut(s)	273, 387, 879, 1003, 1047, 1242, 1476, 1644
ApeKI	GCWGC	8 cut(s)	50, 83, 159, 511, 897, 1063, 1198, 1678
ApoI	RAATTY	2 cut(s)	1693, 1705
ArsI	GACNNNNNNTTYG	2 cut(s)	359, 391
AsiGI	ACCGGT	1 cut(s)	929
AspLEI	GCGC	3 cut(s)	144, 350, 897
AspS9I	GGNCC	4 cut(s)	735, 1022, 1234, 1477
AsuC2I	CCSGG	4 cut(s)	383, 481, 1304, 1741
AsuHPI	GGTGA	8 cut(s)	49, 826, 1000, 1270, 1370, 1433, 1466, 1691
AsuII	TTCGAA	1 cut(s)	1650
AvaI	CYCGRG	2 cut(s)	500, 875
AvaII	GGWCC	3 cut(s)	735, 1022, 1234
BalI	TGGCCA	1 cut(s)	389
BamHI	GGATCC	1 cut(s)	568
BanI	GGYRCC	4 cut(s)	141, 265, 347, 1401
BanII	GRGCYC	1 cut(s)	432
BauI	CACGAG	1 cut(s)	890
BbsI	GAAGAC	1 cut(s)	349
Bbv12I	GWGCWC	3 cut(s)	288, 853, 940
BbvCI	CCTCAGC	1 cut(s)	1628
BbvI	GCAGC	8 cut(s)	37, 70, 146, 523, 884, 1050, 1185, 1690
BccI	CCATC	3 cut(s)	302, 863, 1301
BceAI	ACGGC	6 cut(s)	427, 745, 958, 1189, 1282, 1670
BciT130I	CCWGG	3 cut(s)	224, 668, 830
BciVI	GTATCC	1 cut(s)	1141
BcnI	CCSGG	4 cut(s)	383, 481, 1304, 1741
BcoDI	GTCTC	3 cut(s)	173, 913, 1451
BfaI	CTAG	1 cut(s)	1415
BfoI	RGCGCY	3 cut(s)	145, 351, 898
BfrI	CTTAAG	1 cut(s)	646
BfuI	GTATCC	1 cut(s)	1141
BglI	GCCNNNNNGGC	1 cut(s)	395
BlpI	GCTNAGC	1 cut(s)	426
Bme1390I	CCNGG	7 cut(s)	224, 383, 481, 668, 830, 1304, 1741
Bme18I	GGWCC	3 cut(s)	735, 1022, 1234
BmeRI	GACNNNNNGTC	1 cut(s)	830
BmeT110I	CYCGRG	2 cut(s)	500, 875
BmgBI	CACGTC	1 cut(s)	1135
BmgT120I	GGNCC	4 cut(s)	735, 1022, 1234, 1477
BmiI	GGNNCC	9 cut(s)	143, 228, 267, 349, 570, 769, 1236, 1403, 1563
BmrFI	CCNGG	7 cut(s)	224, 383, 481, 668, 830, 1304, 1741
BmsI	GCATC	4 cut(s)	243, 301, 429, 1221
BpiI	GAAGAC	1 cut(s)	349
BplI	GAGNNNNNCTC	4 cut(s)	893, 925, 1150, 1182
Bpu10I	CCTNAGC	2 cut(s)	1628, 1641
Bpu1102I	GCTNAGC	1 cut(s)	426
Bpu14I	TTCGAA	1 cut(s)	1650
BpuEI	CTTGAG	2 cut(s)	980, 1601
BpuMI	CCSGG	4 cut(s)	383, 481, 1304, 1741
BsaHI	GRCGYC	5 cut(s)	142, 348, 552, 1287, 1341
BsaJI	CCNNGG	7 cut(s)	351, 480, 533, 667, 1068, 1182, 1445
BsaWI	WCCGGW	2 cut(s)	929, 1019
BsaXI	ACNNNNNCTCC	4 cut(s)	92, 122, 1064, 1094
Bse118I	RCCGGY	4 cut(s)	275, 929, 1024, 1280
Bse1I	ACTGG	2 cut(s)	373, 390
Bse3DI	GCAATG	3 cut(s)	249, 321, 1440
BseBI	CCWGG	3 cut(s)	224, 668, 830
BseDI	CCNNGG	7 cut(s)	351, 480, 533, 667, 1068, 1182, 1445
BseGI	GGATG	1 cut(s)	1312
BseMI	GCAATG	3 cut(s)	249, 321, 1440
BseMII	CTCAG	4 cut(s)	357, 415, 417, 1619
BseNI	ACTGG	2 cut(s)	373, 390
BseRI	GAGGAG	3 cut(s)	166, 863, 1088
BseXI	GCAGC	8 cut(s)	37, 70, 146, 523, 884, 1050, 1185, 1690
BseYI	CCCAGC	1 cut(s)	946
Bsh1236I	CGCG	3 cut(s)	762, 797, 1447
Bsh1285I	CGRYCG	1 cut(s)	157
BshFI	GGCC	8 cut(s)	275, 389, 881, 1005, 1049, 1244, 1478, 1646
BshNI	GGYRCC	4 cut(s)	141, 265, 347, 1401
BshTI	ACCGGT	1 cut(s)	929
BsiEI	CGRYCG	1 cut(s)	157
BsiHKAI	GWGCWC	3 cut(s)	288, 853, 940
BsiHKCI	CYCGRG	2 cut(s)	500, 875
BslFI	GGGAC	3 cut(s)	365, 527, 1723
BsmAI	GTCTC	3 cut(s)	173, 913, 1451
BsmBI	CGTCTC	2 cut(s)	173, 913
BsmFI	GGGAC	3 cut(s)	365, 527, 1723
BsnI	GGCC	8 cut(s)	275, 389, 881, 1005, 1049, 1244, 1478, 1646
BsoBI	CYCGRG	2 cut(s)	500, 875
Bsp119I	TTCGAA	1 cut(s)	1650
Bsp1286I	GDGCHC	4 cut(s)	288, 432, 853, 940
Bsp1407I	TGTACA	1 cut(s)	624
Bsp143I	GATC	3 cut(s)	456, 568, 903
Bsp1720I	GCTNAGC	1 cut(s)	426
Bsp19I	CCATGG	1 cut(s)	1182
BspANI	GGCC	8 cut(s)	275, 389, 881, 1005, 1049, 1244, 1478, 1646
BspCNI	CTCAG	4 cut(s)	356, 414, 418, 1620
BspFNI	CGCG	3 cut(s)	762, 797, 1447
BspHI	TCATGA	1 cut(s)	1215
BspLI	GGNNCC	9 cut(s)	143, 228, 267, 349, 570, 769, 1236, 1403, 1563
BspPI	GGATC	2 cut(s)	563, 576
BspT104I	TTCGAA	1 cut(s)	1650
BspT107I	GGYRCC	4 cut(s)	141, 265, 347, 1401
BspTI	CTTAAG	1 cut(s)	646
BsrDI	GCAATG	3 cut(s)	249, 321, 1440
BsrFI	RCCGGY	4 cut(s)	275, 929, 1024, 1280
BsrGI	TGTACA	1 cut(s)	624
BsrI	ACTGG	2 cut(s)	373, 390
BssAI	RCCGGY	4 cut(s)	275, 929, 1024, 1280
BssECI	CCNNGG	7 cut(s)	351, 480, 533, 667, 1068, 1182, 1445
BssMI	GATC	3 cut(s)	456, 568, 903
BssNI	GRCGYC	5 cut(s)	142, 348, 552, 1287, 1341
BssSI	CACGAG	1 cut(s)	890
BssT1I	CCWWGG	3 cut(s)	351, 1068, 1182
Bst2BI	CACGAG	1 cut(s)	890
Bst2UI	CCWGG	3 cut(s)	224, 668, 830
Bst4CI	ACNGT	1 cut(s)	537
Bst6I	CTCTTC	1 cut(s)	71
BstACI	GRCGYC	5 cut(s)	142, 348, 552, 1287, 1341
BstAFI	CTTAAG	1 cut(s)	646
BstAUI	TGTACA	1 cut(s)	624
BstBI	TTCGAA	1 cut(s)	1650
BstC8I	GCNNGC	5 cut(s)	689, 760, 1047, 1391, 1683
BstDEI	CTNAG	6 cut(s)	343, 401, 426, 1481, 1628, 1641
BstDSI	CCRYGG	3 cut(s)	533, 1182, 1445
BstEII	GGTNACC	1 cut(s)	832
BstF5I	GGATG	1 cut(s)	1312
BstFNI	CGCG	3 cut(s)	762, 797, 1447
BstH2I	RGCGCY	3 cut(s)	145, 351, 898
BstHHI	GCGC	3 cut(s)	144, 350, 897
BstKTI	GATC	3 cut(s)	459, 571, 906
BstMAI	GTCTC	3 cut(s)	173, 913, 1451
BstMBI	GATC	3 cut(s)	456, 568, 903
BstMCI	CGRYCG	1 cut(s)	157
BstMWI	GCNNNNNNNGC	7 cut(s)	47, 395, 1399, 1430, 1444, 1475, 1591
BstNI	CCWGG	3 cut(s)	224, 668, 830
BstNSI	RCATGY	2 cut(s)	844, 998
BstPI	GGTNACC	1 cut(s)	832
BstSCI	CCNGG	7 cut(s)	222, 381, 479, 666, 828, 1302, 1739
BstUI	CGCG	3 cut(s)	762, 797, 1447
BstV1I	GCAGC	8 cut(s)	37, 70, 146, 523, 884, 1050, 1185, 1690
BstV2I	GAAGAC	1 cut(s)	349
BstX2I	RGATCY	1 cut(s)	568
BstXI	CCANNNNNNTGG	1 cut(s)	1307
BstYI	RGATCY	1 cut(s)	568
BsuI	GTATCC	1 cut(s)	1141
BsuRI	GGCC	8 cut(s)	275, 389, 881, 1005, 1049, 1244, 1478, 1646
BtgI	CCRYGG	3 cut(s)	533, 1182, 1445
BtrI	CACGTC	1 cut(s)	1135
BtsCI	GGATG	1 cut(s)	1312
BtsI	GCAGTG	1 cut(s)	721
BtsIMutI	CAGTG	2 cut(s)	397, 721
Cac8I	GCNNGC	5 cut(s)	689, 760, 1047, 1391, 1683
CaiI	CAGNNNCTG	1 cut(s)	1729
CciI	TCATGA	1 cut(s)	1215
CfoI	GCGC	3 cut(s)	144, 350, 897
Cfr10I	RCCGGY	4 cut(s)	275, 929, 1024, 1280
Cfr13I	GGNCC	4 cut(s)	735, 1022, 1234, 1477
Cfr42I	CCGCGG	1 cut(s)	1448
CpoI	CGGWCCG	1 cut(s)	1022
CseI	GACGC	1 cut(s)	75
CsiI	ACCWGGT	1 cut(s)	828
Csp6I	GTAC	3 cut(s)	247, 505, 625
CspAI	ACCGGT	1 cut(s)	929
CspI	CGGWCCG	1 cut(s)	1022
CviAII	CATG	6 cut(s)	206, 436, 841, 995, 1183, 1216
CviQI	GTAC	3 cut(s)	247, 505, 625
DdeI	CTNAG	6 cut(s)	343, 401, 426, 1481, 1628, 1641
DinI	GGCGCC	2 cut(s)	143, 349
DpnI	GATC	3 cut(s)	458, 570, 905
DpnII	GATC	3 cut(s)	456, 568, 903
DriI	GACNNNNNGTC	1 cut(s)	830
EaeI	YGGCCR	2 cut(s)	273, 387
Eam1104I	CTCTTC	1 cut(s)	71
Eam1105I	GACNNNNNGTC	1 cut(s)	830
EarI	CTCTTC	1 cut(s)	71
EciI	GGCGGA	1 cut(s)	780
Eco130I	CCWWGG	3 cut(s)	351, 1068, 1182
Eco147I	AGGCCT	1 cut(s)	1646
Eco24I	GRGCYC	1 cut(s)	432
Eco32I	GATATC	1 cut(s)	1522
Eco47I	GGWCC	3 cut(s)	735, 1022, 1234
Eco47III	AGCGCT	1 cut(s)	896
Eco57I	CTGAAG	1 cut(s)	1032
Eco88I	CYCGRG	2 cut(s)	500, 875
Eco91I	GGTNACC	1 cut(s)	832
EcoO109I	RGGNCCY	1 cut(s)	1234
EcoO65I	GGTNACC	1 cut(s)	832
EcoRI	GAATTC	1 cut(s)	1693
EcoRII	CCWGG	3 cut(s)	222, 666, 828
EcoRV	GATATC	1 cut(s)	1522
EcoT14I	CCWWGG	3 cut(s)	351, 1068, 1182
EcoT22I	ATGCAT	1 cut(s)	1000
EcoT38I	GRGCYC	1 cut(s)	432
EgeI	GGCGCC	2 cut(s)	143, 349
EheI	GGCGCC	2 cut(s)	143, 349
ErhI	CCWWGG	3 cut(s)	351, 1068, 1182
Esp3I	CGTCTC	2 cut(s)	173, 913
FaeI	CATG	6 cut(s)	209, 439, 844, 998, 1186, 1219
FalI	AAGNNNNNCTT	4 cut(s)	344, 376, 1211, 1243
FaqI	GGGAC	3 cut(s)	365, 527, 1723
FatI	CATG	6 cut(s)	205, 435, 840, 994, 1182, 1215
FauI	CCCGC	2 cut(s)	311, 1452
FauNDI	CATATG	1 cut(s)	1000
FblI	GTMKAC	2 cut(s)	549, 1659
FokI	GGATG	1 cut(s)	1319
FriOI	GRGCYC	1 cut(s)	432
FspBI	CTAG	1 cut(s)	1415
GlaI	GCGC	3 cut(s)	143, 349, 896
GsaI	CCCAGC	1 cut(s)	950
HaeII	RGCGCY	3 cut(s)	145, 351, 898
HaeIII	GGCC	8 cut(s)	275, 389, 881, 1005, 1049, 1244, 1478, 1646
HgaI	GACGC	1 cut(s)	75
HhaI	GCGC	3 cut(s)	144, 350, 897
Hin1I	GRCGYC	5 cut(s)	142, 348, 552, 1287, 1341
Hin1II	CATG	6 cut(s)	209, 439, 844, 998, 1186, 1219
Hin6I	GCGC	3 cut(s)	142, 348, 895
HinP1I	GCGC	3 cut(s)	142, 348, 895
HincII	GTYRAC	3 cut(s)	550, 1132, 1660
HindII	GTYRAC	3 cut(s)	550, 1132, 1660
HphI	GGTGA	8 cut(s)	49, 826, 1000, 1270, 1370, 1433, 1466, 1691
Hpy166II	GTNNAC	7 cut(s)	367, 550, 625, 1132, 1300, 1504, 1660
Hpy188I	TCNGA	6 cut(s)	404, 607, 1147, 1519, 1566, 1669
Hpy188III	TCNNGA	7 cut(s)	73, 454, 1169, 1216, 1261, 1415, 1690
Hpy8I	GTNNAC	7 cut(s)	367, 550, 625, 1132, 1300, 1504, 1660
HpyAV	CCTTC	5 cut(s)	116, 748, 1132, 1213, 1657
HpyCH4III	ACNGT	1 cut(s)	537
HpyCH4IV	ACGT	9 cut(s)	552, 774, 1128, 1134, 1287, 1341, 1500, 1506, 1662
HpyCH4V	TGCA	3 cut(s)	256, 726, 998
HpyF10VI	GCNNNNNNNGC	7 cut(s)	47, 395, 1399, 1430, 1444, 1475, 1591
HpyF3I	CTNAG	6 cut(s)	343, 401, 426, 1481, 1628, 1641
HpySE526I	ACGT	9 cut(s)	552, 774, 1128, 1134, 1287, 1341, 1500, 1506, 1662
Hsp92I	GRCGYC	5 cut(s)	142, 348, 552, 1287, 1341
Hsp92II	CATG	6 cut(s)	209, 439, 844, 998, 1186, 1219
HspAI	GCGC	3 cut(s)	142, 348, 895
KasI	GGCGCC	2 cut(s)	141, 347
KspI	CCGCGG	1 cut(s)	1448
Kzo9I	GATC	3 cut(s)	456, 568, 903
LmnI	GCTCC	6 cut(s)	232, 684, 856, 935, 955, 1075
Lsp1109I	GCAGC	8 cut(s)	37, 70, 146, 523, 884, 1050, 1185, 1690
LweI	GCATC	4 cut(s)	243, 301, 429, 1221
MabI	ACCWGGT	1 cut(s)	828
MaeI	CTAG	1 cut(s)	1415
MaeII	ACGT	9 cut(s)	552, 774, 1128, 1134, 1287, 1341, 1500, 1506, 1662
MaeIII	GTNAC	3 cut(s)	832, 988, 1223
MalI	GATC	3 cut(s)	458, 570, 905
MboI	GATC	3 cut(s)	456, 568, 903
MboII	GAAGA	6 cut(s)	88, 349, 853, 1267, 1552, 1621
MfeI	CAATTG	1 cut(s)	573
MflI	RGATCY	1 cut(s)	568
MhlI	GDGCHC	4 cut(s)	288, 432, 853, 940
MlsI	TGGCCA	1 cut(s)	389
MluCI	AATT	5 cut(s)	573, 1320, 1528, 1693, 1705
MluNI	TGGCCA	1 cut(s)	389
Mly113I	GGCGCC	2 cut(s)	142, 348
MlyI	GAGTC	6 cut(s)	464, 1111, 1136, 1453, 1487, 1523
MmeI	TCCRAC	2 cut(s)	1362, 1521
Mox20I	TGGCCA	1 cut(s)	389
Mph1103I	ATGCAT	1 cut(s)	1000
MscI	TGGCCA	1 cut(s)	389
MseI	TTAA	4 cut(s)	647, 656, 662, 1323
MslI	CAYNNNNRTG	4 cut(s)	636, 839, 999, 1305
Msp20I	TGGCCA	1 cut(s)	389
MspA1I	CMGCKG	2 cut(s)	159, 1447
MspCI	CTTAAG	1 cut(s)	646
MspR9I	CCNGG	7 cut(s)	224, 383, 481, 668, 830, 1304, 1741
MunI	CAATTG	1 cut(s)	573
MvaI	CCWGG	3 cut(s)	224, 668, 830
MvnI	CGCG	3 cut(s)	762, 797, 1447
MwoI	GCNNNNNNNGC	7 cut(s)	47, 395, 1399, 1430, 1444, 1475, 1591
NarI	GGCGCC	2 cut(s)	142, 348
NciI	CCSGG	4 cut(s)	383, 481, 1304, 1741
NcoI	CCATGG	1 cut(s)	1182
NdeI	CATATG	1 cut(s)	1000
NdeII	GATC	3 cut(s)	456, 568, 903
NlaIII	CATG	6 cut(s)	209, 439, 844, 998, 1186, 1219
NlaIV	GGNNCC	9 cut(s)	143, 228, 267, 349, 570, 769, 1236, 1403, 1563
NmuCI	GTSAC	2 cut(s)	832, 988
NsiI	ATGCAT	1 cut(s)	1000
NspI	RCATGY	2 cut(s)	844, 998
NspV	TTCGAA	1 cut(s)	1650
OliI	CACNNNNGTG	1 cut(s)	636
PaeR7I	CTCGAG	2 cut(s)	500, 875
PagI	TCATGA	1 cut(s)	1215
PceI	AGGCCT	1 cut(s)	1646
PciI	ACATGT	1 cut(s)	840
PcsI	WCGNNNNNNNCGW	9 cut(s)	459, 465, 546, 555, 558, 897, 1253, 1497, 1656
PfeI	GAWTC	4 cut(s)	405, 1084, 1612, 1726
PflMI	CCANNNNNTGG	2 cut(s)	211, 1709
PinAI	ACCGGT	1 cut(s)	929
PleI	GAGTC	6 cut(s)	464, 1111, 1136, 1453, 1487, 1522
PluTI	GGCGCC	2 cut(s)	145, 351
PpsI	GAGTC	6 cut(s)	464, 1111, 1136, 1453, 1487, 1522
PpuMI	RGGWCCY	1 cut(s)	1234
PscI	ACATGT	1 cut(s)	840
Psp5II	RGGWCCY	1 cut(s)	1234
Psp6I	CCWGG	3 cut(s)	222, 666, 828
PspEI	GGTNACC	1 cut(s)	832
PspFI	CCCAGC	1 cut(s)	946
PspGI	CCWGG	3 cut(s)	222, 666, 828
PspN4I	GGNNCC	9 cut(s)	143, 228, 267, 349, 570, 769, 1236, 1403, 1563
PspPI	GGNCC	4 cut(s)	735, 1022, 1234, 1477
PspPPI	RGGWCCY	1 cut(s)	1234
PspXI	VCTCGAGB	2 cut(s)	500, 875
PstNI	CAGNNNCTG	1 cut(s)	1729
PsuI	RGATCY	1 cut(s)	568
RsaI	GTAC	3 cut(s)	248, 506, 626
RsaNI	GTAC	3 cut(s)	247, 505, 625
RseI	CAYNNNNRTG	4 cut(s)	636, 839, 999, 1305
Rsr2I	CGGWCCG	1 cut(s)	1022
RsrII	CGGWCCG	1 cut(s)	1022
SacII	CCGCGG	1 cut(s)	1448
SalI	GTCGAC	2 cut(s)	548, 1658
SaqAI	TTAA	4 cut(s)	647, 656, 662, 1323
Sau3AI	GATC	3 cut(s)	456, 568, 903
Sau96I	GGNCC	4 cut(s)	735, 1022, 1234, 1477
SchI	GAGTC	6 cut(s)	464, 1111, 1136, 1453, 1487, 1523
ScrFI	CCNGG	7 cut(s)	224, 383, 481, 668, 830, 1304, 1741
SduI	GDGCHC	4 cut(s)	288, 432, 853, 940
SexAI	ACCWGGT	1 cut(s)	828
SfaNI	GCATC	4 cut(s)	243, 301, 429, 1221
SfoI	GGCGCC	2 cut(s)	143, 349
Sfr274I	CTCGAG	2 cut(s)	500, 875
Sfr303I	CCGCGG	1 cut(s)	1448
SfuI	TTCGAA	1 cut(s)	1650
SgrAI	CRCCGGYG	1 cut(s)	1280
SgrBI	CCGCGG	1 cut(s)	1448
SgrDI	CGTCGACG	2 cut(s)	548, 1658
SinI	GGWCC	3 cut(s)	735, 1022, 1234
SlaI	CTCGAG	2 cut(s)	500, 875
SmiMI	CAYNNNNRTG	4 cut(s)	636, 839, 999, 1305
SmlI	CTYRAG	5 cut(s)	500, 646, 875, 959, 1616
SmoI	CTYRAG	5 cut(s)	500, 646, 875, 959, 1616
Sse9I	AATT	5 cut(s)	573, 1320, 1528, 1693, 1705
SseBI	AGGCCT	1 cut(s)	1646
SspDI	GGCGCC	2 cut(s)	141, 347
SspMI	CTAG	1 cut(s)	1415
StuI	AGGCCT	1 cut(s)	1646
StyD4I	CCNGG	7 cut(s)	222, 381, 479, 666, 828, 1302, 1739
StyI	CCWWGG	3 cut(s)	351, 1068, 1182
TaaI	ACNGT	1 cut(s)	537
TaiI	ACGT	9 cut(s)	555, 777, 1131, 1137, 1290, 1344, 1503, 1509, 1665
TasI	AATT	5 cut(s)	573, 1320, 1528, 1693, 1705
TatI	WGTACW	1 cut(s)	624
TauI	GCSGC	5 cut(s)	758, 765, 1424, 1427, 1552
TfiI	GAWTC	4 cut(s)	405, 1084, 1612, 1726
Tru1I	TTAA	4 cut(s)	647, 656, 662, 1323
Tru9I	TTAA	4 cut(s)	647, 656, 662, 1323
TscAI	CASTG	2 cut(s)	397, 728
TseFI	GTSAC	2 cut(s)	832, 988
TseI	GCWGC	8 cut(s)	50, 83, 159, 511, 897, 1063, 1198, 1678
Tsp45I	GTSAC	2 cut(s)	832, 988
TspDTI	ATGAA	3 cut(s)	32, 428, 1232
TspGWI	ACGGA	2 cut(s)	23, 942
TspRI	CASTG	2 cut(s)	397, 728
Van91I	CCANNNNNTGG	2 cut(s)	211, 1709
Vha464I	CTTAAG	1 cut(s)	646
VpaK11BI	GGWCC	3 cut(s)	735, 1022, 1234
XapI	RAATTY	2 cut(s)	1693, 1705
XbaI	TCTAGA	1 cut(s)	1414
XceI	RCATGY	2 cut(s)	844, 998
XhoI	CTCGAG	2 cut(s)	500, 875
XmiI	GTMKAC	2 cut(s)	549, 1659
XspI	CTAG	1 cut(s)	1415
ZraI	GACGTC	3 cut(s)	553, 1288, 1342
Zsp2I	ATGCAT	1 cut(s)	1000

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.