Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0003881

Physical Location & Seq

Genomic Coordinates Reverse (-)

19599155 .. 19601045

Gene Structure 1891 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g18850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGGCCCTTGAATCTATGAATATGCTTCCGAAATGTGATGCCAATTACGCAGCTCTCTCTCCCACAACCTTTCTCAAGAGAGCTGCTGCTTTTTATGCCGACCGTACCTCAGTCGTCTATGAAGGCACTCGCTTCACTTGGGCCCAAACCTACGACCGTTGCTGCCGTCTCGCTTCCTCCCTCGTTTCCCTCAACATCGTCAAACACGATGTGGTTTCAGTATTGGCTCCTAACATTCCGGCAATGTACGAAATGCATTTCGGAGTGCCCATGGCCGGGGCCGTGCTCAACACCGTCAACACCCGCCTGGACGCCAAAACCATAGCCTCCATTCTCCGGCACTCAGGAGCCAAAGTCTTCTTTGTCGACTACCAATTTGTCCCCCTGGCACGTGACATCCTGAGGATTCTCACCACCGGAACTGCCGGCACATCCATGCCGTTGGTCATCGTGATCGACGATATCGACTCCCCCACTGGTATTCGACTCGGCGAGTTGGAGTACGAGCAACTCATCAAAAGGGGCAATCCCGGATTTATCTCCGTTCCAGTCGATGACGAGTGGGACCCCGTCGCTTTGAATTACACCTCCGGAACGACATCGGAGCCCAAAGGCGTTGTGTACAGCCACAGAGGCGCATTCTTGAGCACCCTGAGCCTAATTTTGGGCTGGGAAATGGGGGGCGAGCCTGTTTATTTGTGGACCCTCCCCATGTTTCATTGCAACGGCTGGACCTTCACGTGGGGCGTCGCAGCACGCGGCGGCACCAACGTCTGCCTCCGCAACACCACTGCCTACGACATCTACCGCAACATTCACCGCCACAAGGTGACGCACATGTGCTGCGCCCCCATCATCTTCAACATCCTCCTCGAGGCCAAAACCCACGAGCGCTGCGAGATCTCAACGCCCGTTCAGATACTCACTGGCGGAGCCCCTCCTCCGGCCCCTCTCCTAAAGAAGATCGAGCCGCTAGGGTTTAAAGTAACCCACGCGTACGGCCTGACAGAGGCAACAGGCCCCGCTTTGGTCTGCGAATGGCAGGCCAAGTGGAACAAGCTGTCTCAGGATGGTCAGGCCAAACTCAAGGCAAGGCAAGGCATAAGCATATTGACTCTTGCTGACGTGGATGTAAAAAACAAGGACACAATGGAGAGTGTCCCCCATGACGGGAAGACCATGGGGGAGATTGTTTTGCGTGGGAGTAGCATCATGAAAGGGTATTTCAAGGATCCCAAGGCGACCAAGAAGGCGTTCCAGAACGGATGGTTCTGGACTGGAGACGTCGGGGTTGTACACCCAGACGGCTACATGGAGATAAAGGACAGGTCGAAAGATGTGATCATATCAGGAGGAGAAAATATTAGCAGTGTGGAAGTTGAAAATATGTTACATGGGCATCCAAAGGTGTTAGAGGCAGCAGTTGTGGCAATGCCACACTCTCGATGGGGAGAGAGCCCGTGTGCTTTTGTGGCTCTCCGAAGCAACGCCGAGGGGGCAGCCACCGAGTCCGAAATCATTGCTTACTGCCGCAAAAACTTGCCGCATTTTATGGTGCCCAAGAAGGTGGAGTTTCTGCCCCAGCTGCCCAGAAATCCAATGGGCAAGGTTCTCAAAAACGTTTTGAGGGATCAGGCAAAGACATTCATCTCATCGGAAAGTCAACAGACCGCCGTTGATTCCCACAGCAATGATCAGCAGATTCTTGCTTTGTCGCGTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0016143 GO:0016144 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018858 GO:0019605 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046459 GO:0047760 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

575

Amino Acids

63.28

Weight (kDa)

8.18

Isoelectric Point (pI)

39.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	26 - 409	7.3e-83	AMP-binding enzyme
AMP-binding_C	PF13193	459 - 536	1.2e-21	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65880 AT1G65890 AT1G66120
fragaria_vesca	FvH4_1g27440 FvH4_1g27450
malus_domestica	MD09G1280400.v1.1 MD17G1287600.v1.1
prunus_persica	Prupe.3G013700_v2.0.a1
pyrus_communis	pycom09g18850 pycom17g27060
rosa_chinensis	RchiOBHm_Chr3g0488561 RchiOBHm_Chr3g0488571
rosa_laevigata	RLG00000022942 RLG00000022943
rosa_multiflora	Rmu_sc0004402.1_g000006 Rmu_sc0004402.1_g000007 Rmu_sc0005363.1_g000024 Rmu_sc0005363.1_g000025
rosa_roxburghii	Rroxscaffold_6G00394130 Rroxscaffold_6G00394140
rosa_rugosa	Rorug03G0239300 Rorug03G0239400
rosa_samantha	Rh3AG288300 Rh3AG288400 Rh3BG325000 Rh3BG325100 Rh3CG322200 Rh3CG322300 Rh3DG321000 Rh3DG321100 Rh4AG012700 Rh7BG359000
rosa_wichuraiana	Rw3G025510 Rw3G025520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1287
AbsI	CCTCGAGG	1 cut(s)	872
AccB1I	GGYRCC	2 cut(s)	764, 1555
AccI	GTMKAC	1 cut(s)	366
AccII	CGCG	3 cut(s)	759, 995, 1717
AccIII	TCCGGA	1 cut(s)	590
AclI	AACGTT	1 cut(s)	1620
AclWI	GGATC	3 cut(s)	1226, 1239, 1638
AcoI	YGGCCR	1 cut(s)	273
AcvI	CACGTG	2 cut(s)	392, 741
AcyI	GRCGYC	3 cut(s)	312, 747, 1284
AdeI	CACNNNGTG	2 cut(s)	211, 829
AfaI	GTAC	6 cut(s)	106, 248, 503, 623, 998, 1296
AfeI	AGCGCT	1 cut(s)	893
AflIII	ACRYGT	2 cut(s)	837, 993
AgsI	TTSAA	5 cut(s)	11, 580, 862, 1228, 1382
AjiI	CACGTC	1 cut(s)	1126
AjnI	CCWGG	2 cut(s)	306, 384
AjuI	GAANNNNNNNTTGG	2 cut(s)	1238, 1270
AluBI	AGCT	4 cut(s)	53, 83, 1060, 1585
AluI	AGCT	4 cut(s)	53, 83, 1060, 1585
Alw21I	GWGCWC	2 cut(s)	288, 650
Alw26I	GTCTC	3 cut(s)	173, 1068, 1275
AlwI	GGATC	3 cut(s)	1226, 1239, 1638
Ama87I	CYCGRG	1 cut(s)	872
Aor13HI	TCCGGA	1 cut(s)	590
Aor51HI	AGCGCT	1 cut(s)	893
ApaI	GGGCCC	1 cut(s)	145
ArsI	GACNNNNNNTTYG	2 cut(s)	359, 391
Asp700I	GAANNNNTTC	1 cut(s)	1253
AspLEI	GCGC	3 cut(s)	638, 848, 894
AspS9I	GGNCC	9 cut(s)	4, 141, 142, 279, 565, 702, 732, 946, 1019
AsuC2I	CCSGG	2 cut(s)	277, 531
AsuHPI	GGTGA	3 cut(s)	403, 809, 841
AvaI	CYCGRG	1 cut(s)	872
AvaII	GGWCC	3 cut(s)	565, 702, 732
AxyI	CCTNAGG	1 cut(s)	401
BaeGI	GKGCMC	3 cut(s)	145, 270, 1560
BamHI	GGATCC	1 cut(s)	1231
BanI	GGYRCC	2 cut(s)	764, 1555
BanII	GRGCYC	4 cut(s)	145, 609, 937, 1460
BauI	CACGAG	1 cut(s)	887
BbrPI	CACGTG	2 cut(s)	392, 741
BbsI	GAAGAC	2 cut(s)	349, 1181
Bbv12I	GWGCWC	2 cut(s)	288, 650
BccI	CCATC	4 cut(s)	860, 1064, 1260, 1440
BceAI	ACGGC	7 cut(s)	150, 266, 424, 742, 1015, 1321, 1658
BcgI	CGANNNNNNTGC	2 cut(s)	1502, 1536
BciT130I	CCWGG	2 cut(s)	308, 386
BclI	TGATCA	2 cut(s)	1341, 1693
BcnI	CCSGG	2 cut(s)	277, 531
BcoDI	GTCTC	3 cut(s)	173, 1068, 1275
BfaI	CTAG	1 cut(s)	974
BfoI	RGCGCY	1 cut(s)	895
BglI	GCCNNNNNGGC	2 cut(s)	633, 1496
BglII	AGATCT	1 cut(s)	900
Bme1390I	CCNGG	4 cut(s)	277, 308, 386, 531
Bme18I	GGWCC	3 cut(s)	565, 702, 732
BmeT110I	CYCGRG	1 cut(s)	872
BmgBI	CACGTC	1 cut(s)	1126
BmgT120I	GGNCC	9 cut(s)	4, 141, 142, 279, 565, 702, 732, 946, 1019
BmrFI	CCNGG	4 cut(s)	277, 308, 386, 531
BmsI	GCATC	3 cut(s)	28, 1218, 1408
BpiI	GAAGAC	2 cut(s)	349, 1181
BpmI	CTGGAG	1 cut(s)	1299
Bpu10I	CCTNAGC	1 cut(s)	653
BpuEI	CTTGAG	3 cut(s)	59, 664, 1070
BpuMI	CCSGG	2 cut(s)	277, 531
BsaAI	YACGTR	2 cut(s)	392, 741
BsaHI	GRCGYC	3 cut(s)	312, 747, 1284
BsaJI	CCNNGG	6 cut(s)	270, 276, 384, 1179, 1236, 1491
BsaWI	WCCGGW	2 cut(s)	416, 590
BsaXI	ACNNNNNCTCC	2 cut(s)	339, 369
Bse118I	RCCGGY	1 cut(s)	425
Bse1I	ACTGG	4 cut(s)	481, 548, 931, 1282
Bse21I	CCTNAGG	1 cut(s)	401
Bse3DI	GCAATG	5 cut(s)	249, 718, 1437, 1518, 1696
BseAI	TCCGGA	1 cut(s)	590
BseBI	CCWGG	2 cut(s)	308, 386
BseDI	CCNNGG	6 cut(s)	270, 276, 384, 1179, 1236, 1491
BseGI	GGATG	7 cut(s)	396, 431, 864, 1075, 1135, 1271, 1399
BseMI	GCAATG	5 cut(s)	249, 718, 1437, 1518, 1696
BseMII	CTCAG	5 cut(s)	123, 357, 392, 644, 1079
BseNI	ACTGG	4 cut(s)	481, 548, 931, 1282
BseRI	GAGGAG	3 cut(s)	860, 930, 1368
BseSI	GKGCMC	3 cut(s)	145, 270, 1560
BseYI	CCCAGC	2 cut(s)	669, 1581
Bsh1236I	CGCG	3 cut(s)	759, 995, 1717
Bsh1285I	CGRYCG	2 cut(s)	103, 157
BshNI	GGYRCC	2 cut(s)	764, 1555
BsiEI	CGRYCG	2 cut(s)	103, 157
BsiHKAI	GWGCWC	2 cut(s)	288, 650
BsiHKCI	CYCGRG	1 cut(s)	872
BsiSI	CCGG	8 cut(s)	239, 276, 337, 417, 426, 531, 591, 944
BsiWI	CGTACG	1 cut(s)	996
BslFI	GGGAC	3 cut(s)	365, 578, 1145
BsmAI	GTCTC	3 cut(s)	173, 1068, 1275
BsmBI	CGTCTC	2 cut(s)	173, 1275
BsmFI	GGGAC	3 cut(s)	365, 578, 1145
BsmI	GAATGC	1 cut(s)	638
BsoBI	CYCGRG	1 cut(s)	872
Bsp120I	GGGCCC	1 cut(s)	141
Bsp1286I	GDGCHC	8 cut(s)	145, 270, 288, 609, 650, 937, 1460, 1560
Bsp13I	TCCGGA	1 cut(s)	590
Bsp1407I	TGTACA	2 cut(s)	621, 1294
Bsp143I	GATC	7 cut(s)	453, 900, 963, 1231, 1341, 1630, 1693
Bsp19I	CCATGG	2 cut(s)	270, 1179
BspCNI	CTCAG	5 cut(s)	122, 356, 393, 645, 1078
BspEI	TCCGGA	1 cut(s)	590
BspFNI	CGCG	3 cut(s)	759, 995, 1717
BspHI	TCATGA	1 cut(s)	1212
BspPI	GGATC	3 cut(s)	1226, 1239, 1638
BspT107I	GGYRCC	2 cut(s)	764, 1555
BsrDI	GCAATG	5 cut(s)	249, 718, 1437, 1518, 1696
BsrFI	RCCGGY	1 cut(s)	425
BsrGI	TGTACA	2 cut(s)	621, 1294
BsrI	ACTGG	4 cut(s)	481, 548, 931, 1282
BssAI	RCCGGY	1 cut(s)	425
BssECI	CCNNGG	6 cut(s)	270, 276, 384, 1179, 1236, 1491
BssMI	GATC	7 cut(s)	453, 900, 963, 1231, 1341, 1630, 1693
BssNI	GRCGYC	3 cut(s)	312, 747, 1284
BssSI	CACGAG	1 cut(s)	887
BssT1I	CCWWGG	3 cut(s)	270, 1179, 1236
Bst2BI	CACGAG	1 cut(s)	887
Bst2UI	CCWGG	2 cut(s)	308, 386
Bst4CI	ACNGT	3 cut(s)	104, 158, 295
BstACI	GRCGYC	3 cut(s)	312, 747, 1284
BstAUI	TGTACA	2 cut(s)	621, 1294
BstBAI	YACGTR	2 cut(s)	392, 741
BstC8I	GCNNGC	4 cut(s)	427, 686, 757, 1044
BstDEI	CTNAG	5 cut(s)	109, 343, 401, 653, 1065
BstDSI	CCRYGG	2 cut(s)	270, 1179
BstENI	CCTNNNNNAGG	2 cut(s)	872, 1007
BstF5I	GGATG	7 cut(s)	396, 431, 864, 1075, 1135, 1271, 1399
BstFNI	CGCG	3 cut(s)	759, 995, 1717
BstH2I	RGCGCY	1 cut(s)	895
BstHHI	GCGC	3 cut(s)	638, 848, 894
BstKTI	GATC	7 cut(s)	456, 903, 966, 1234, 1344, 1633, 1696
BstMAI	GTCTC	3 cut(s)	173, 1068, 1275
BstMBI	GATC	7 cut(s)	453, 900, 963, 1231, 1341, 1630, 1693
BstMCI	CGRYCG	2 cut(s)	103, 157
BstMWI	GCNNNNNNNGC	8 cut(s)	47, 95, 633, 654, 1427, 1472, 1496, 1585
BstNI	CCWGG	2 cut(s)	308, 386
BstNSI	RCATGY	1 cut(s)	841
BstSCI	CCNGG	4 cut(s)	275, 306, 384, 529
BstSLI	GKGCMC	3 cut(s)	145, 270, 1560
BstUI	CGCG	3 cut(s)	759, 995, 1717
BstV2I	GAAGAC	2 cut(s)	349, 1181
BstX2I	RGATCY	2 cut(s)	900, 1231
BstXI	CCANNNNNNTGG	2 cut(s)	442, 1567
BstYI	RGATCY	2 cut(s)	900, 1231
Bsu36I	CCTNAGG	1 cut(s)	401
BtgI	CCRYGG	2 cut(s)	270, 1179
BtrI	CACGTC	1 cut(s)	1126
BtsCI	GGATG	7 cut(s)	396, 431, 864, 1075, 1135, 1271, 1399
BtsI	GCAGTG	2 cut(s)	789, 1375
BtsIMutI	CAGTG	4 cut(s)	474, 789, 924, 1375
Cac8I	GCNNGC	4 cut(s)	427, 686, 757, 1044
CciI	TCATGA	1 cut(s)	1212
CfoI	GCGC	3 cut(s)	638, 848, 894
Cfr10I	RCCGGY	1 cut(s)	425
Cfr13I	GGNCC	9 cut(s)	4, 141, 142, 279, 565, 702, 732, 946, 1019
CseI	GACGC	4 cut(s)	320, 736, 841, 1706
Csp6I	GTAC	6 cut(s)	105, 247, 502, 622, 997, 1295
CviAII	CATG	9 cut(s)	271, 436, 712, 838, 1166, 1180, 1213, 1312, 1394
CviQI	GTAC	6 cut(s)	105, 247, 502, 622, 997, 1295
DdeI	CTNAG	5 cut(s)	109, 343, 401, 653, 1065
DpnI	GATC	7 cut(s)	455, 902, 965, 1233, 1343, 1632, 1695
DpnII	GATC	7 cut(s)	453, 900, 963, 1231, 1341, 1630, 1693
DraI	TTTAAA	1 cut(s)	982
DraIII	CACNNNGTG	2 cut(s)	211, 829
EaeI	YGGCCR	1 cut(s)	273
EciI	GGCGGA	1 cut(s)	945
Eco130I	CCWWGG	3 cut(s)	270, 1179, 1236
Eco24I	GRGCYC	4 cut(s)	145, 609, 937, 1460
Eco32I	GATATC	1 cut(s)	463
Eco47I	GGWCC	3 cut(s)	565, 702, 732
Eco47III	AGCGCT	1 cut(s)	893
Eco72I	CACGTG	2 cut(s)	392, 741
Eco81I	CCTNAGG	1 cut(s)	401
Eco88I	CYCGRG	1 cut(s)	872
EcoNI	CCTNNNNNAGG	2 cut(s)	872, 1007
EcoO109I	RGGNCCY	2 cut(s)	565, 1019
EcoRII	CCWGG	2 cut(s)	306, 384
EcoRV	GATATC	1 cut(s)	463
EcoT14I	CCWWGG	3 cut(s)	270, 1179, 1236
EcoT22I	ATGCAT	1 cut(s)	258
EcoT38I	GRGCYC	4 cut(s)	145, 609, 937, 1460
ErhI	CCWWGG	3 cut(s)	270, 1179, 1236
Esp3I	CGTCTC	2 cut(s)	173, 1275
FaeI	CATG	9 cut(s)	274, 439, 715, 841, 1169, 1183, 1216, 1315, 1397
FaqI	GGGAC	3 cut(s)	365, 578, 1145
FatI	CATG	9 cut(s)	270, 435, 711, 837, 1165, 1179, 1212, 1311, 1393
FauI	CCCGC	2 cut(s)	311, 1030
FbaI	TGATCA	2 cut(s)	1341, 1693
FblI	GTMKAC	1 cut(s)	366
FokI	GGATG	7 cut(s)	383, 418, 851, 1082, 1142, 1278, 1386
FriOI	GRGCYC	4 cut(s)	145, 609, 937, 1460
FspBI	CTAG	1 cut(s)	974
GlaI	GCGC	3 cut(s)	637, 847, 893
GsaI	CCCAGC	2 cut(s)	673, 1585
GsuI	CTGGAG	1 cut(s)	1299
HaeII	RGCGCY	1 cut(s)	895
HapII	CCGG	8 cut(s)	239, 276, 337, 417, 426, 531, 591, 944
HgaI	GACGC	4 cut(s)	320, 736, 841, 1706
HhaI	GCGC	3 cut(s)	638, 848, 894
Hin1I	GRCGYC	3 cut(s)	312, 747, 1284
Hin1II	CATG	9 cut(s)	274, 439, 715, 841, 1169, 1183, 1216, 1315, 1397
Hin6I	GCGC	3 cut(s)	636, 846, 892
HinP1I	GCGC	3 cut(s)	636, 846, 892
HincII	GTYRAC	3 cut(s)	298, 367, 1664
HindII	GTYRAC	3 cut(s)	298, 367, 1664
HinfI	GANTC	8 cut(s)	11, 406, 467, 486, 1114, 1508, 1679, 1702
HpaII	CCGG	8 cut(s)	239, 276, 337, 417, 426, 531, 591, 944
HphI	GGTGA	3 cut(s)	403, 809, 841
Hpy166II	GTNNAC	6 cut(s)	298, 367, 622, 702, 1297, 1664
Hpy188I	TCNGA	7 cut(s)	30, 263, 604, 918, 1481, 1513, 1657
Hpy8I	GTNNAC	6 cut(s)	298, 367, 622, 702, 1297, 1664
Hpy99I	CGWCG	4 cut(s)	461, 575, 752, 1289
HpyAV	CCTTC	4 cut(s)	116, 745, 1243, 1558
HpyCH4III	ACNGT	3 cut(s)	104, 158, 295
HpyCH4IV	ACGT	6 cut(s)	391, 740, 771, 1125, 1284, 1620
HpyCH4V	TGCA	2 cut(s)	256, 723
HpyF10VI	GCNNNNNNNGC	8 cut(s)	47, 95, 633, 654, 1427, 1472, 1496, 1585
HpyF3I	CTNAG	5 cut(s)	109, 343, 401, 653, 1065
HpySE526I	ACGT	6 cut(s)	391, 740, 771, 1125, 1284, 1620
Hsp92I	GRCGYC	3 cut(s)	312, 747, 1284
Hsp92II	CATG	9 cut(s)	274, 439, 715, 841, 1169, 1183, 1216, 1315, 1397
HspAI	GCGC	3 cut(s)	636, 846, 892
KflI	GGGWCCC	1 cut(s)	565
Kpn2I	TCCGGA	1 cut(s)	590
KroI	GCCGGC	1 cut(s)	425
KroNI	GCCGGC	1 cut(s)	427
Ksp22I	TGATCA	2 cut(s)	1341, 1693
Kzo9I	GATC	7 cut(s)	453, 900, 963, 1231, 1341, 1630, 1693
LmnI	GCTCC	4 cut(s)	232, 347, 604, 932
LweI	GCATC	3 cut(s)	28, 1218, 1408
MaeI	CTAG	1 cut(s)	974
MaeII	ACGT	6 cut(s)	391, 740, 771, 1125, 1284, 1620
MaeIII	GTNAC	4 cut(s)	392, 829, 985, 1389
MalI	GATC	7 cut(s)	455, 902, 965, 1233, 1343, 1632, 1695
MboI	GATC	7 cut(s)	453, 900, 963, 1231, 1341, 1630, 1693
MboII	GAAGA	4 cut(s)	349, 850, 973, 1186
MflI	RGATCY	2 cut(s)	900, 1231
MhlI	GDGCHC	8 cut(s)	145, 270, 288, 609, 650, 937, 1460, 1560
MluCI	AATT	4 cut(s)	43, 374, 580, 660
MluI	ACGCGT	1 cut(s)	993
MlyI	GAGTC	4 cut(s)	461, 480, 1108, 1517
MmeI	TCCRAC	1 cut(s)	477
Mph1103I	ATGCAT	1 cut(s)	258
MroI	TCCGGA	1 cut(s)	590
MroNI	GCCGGC	1 cut(s)	425
MroXI	GAANNNNTTC	1 cut(s)	1253
MseI	TTAA	1 cut(s)	981
MslI	CAYNNNNRTG	2 cut(s)	434, 1689
MspA1I	CMGCKG	1 cut(s)	1585
MspI	CCGG	8 cut(s)	239, 276, 337, 417, 426, 531, 591, 944
MspR9I	CCNGG	4 cut(s)	277, 308, 386, 531
Mva1269I	GAATGC	1 cut(s)	638
MvaI	CCWGG	2 cut(s)	308, 386
MvnI	CGCG	3 cut(s)	759, 995, 1717
MwoI	GCNNNNNNNGC	8 cut(s)	47, 95, 633, 654, 1427, 1472, 1496, 1585
NaeI	GCCGGC	1 cut(s)	427
NciI	CCSGG	2 cut(s)	277, 531
NcoI	CCATGG	2 cut(s)	270, 1179
NdeII	GATC	7 cut(s)	453, 900, 963, 1231, 1341, 1630, 1693
NgoMIV	GCCGGC	1 cut(s)	425
NlaIII	CATG	9 cut(s)	274, 439, 715, 841, 1169, 1183, 1216, 1315, 1397
NmeAIII	GCCGAG	2 cut(s)	468, 1516
NmuCI	GTSAC	2 cut(s)	392, 829
NsiI	ATGCAT	1 cut(s)	258
NspI	RCATGY	1 cut(s)	841
PaeR7I	CTCGAG	1 cut(s)	872
PagI	TCATGA	1 cut(s)	1212
PciI	ACATGT	1 cut(s)	837
PcsI	WCGNNNNNNNCGW	4 cut(s)	204, 456, 462, 894
PctI	GAATGC	1 cut(s)	638
PdiI	GCCGGC	1 cut(s)	427
PdmI	GAANNNNTTC	1 cut(s)	1253
PfeI	GAWTC	4 cut(s)	11, 406, 1679, 1702
Pfl23II	CGTACG	1 cut(s)	996
PflFI	GACNNNGTC	1 cut(s)	569
PfoI	TCCNGGA	1 cut(s)	529
PleI	GAGTC	4 cut(s)	461, 480, 1108, 1516
PmaCI	CACGTG	2 cut(s)	392, 741
PmlI	CACGTG	2 cut(s)	392, 741
PpsI	GAGTC	4 cut(s)	461, 480, 1108, 1516
Ppu21I	YACGTR	2 cut(s)	392, 741
PpuMI	RGGWCCY	1 cut(s)	565
PscI	ACATGT	1 cut(s)	837
Psp1406I	AACGTT	1 cut(s)	1620
Psp5II	RGGWCCY	1 cut(s)	565
Psp6I	CCWGG	2 cut(s)	306, 384
PspCI	CACGTG	2 cut(s)	392, 741
PspFI	CCCAGC	2 cut(s)	669, 1581
PspGI	CCWGG	2 cut(s)	306, 384
PspLI	CGTACG	1 cut(s)	996
PspOMI	GGGCCC	1 cut(s)	141
PspPI	GGNCC	9 cut(s)	4, 141, 142, 279, 565, 702, 732, 946, 1019
PspPPI	RGGWCCY	1 cut(s)	565
PspXI	VCTCGAGB	1 cut(s)	872
PsuI	RGATCY	2 cut(s)	900, 1231
PsyI	GACNNNGTC	1 cut(s)	569
PvuII	CAGCTG	1 cut(s)	1585
RsaI	GTAC	6 cut(s)	106, 248, 503, 623, 998, 1296
RsaNI	GTAC	6 cut(s)	105, 247, 502, 622, 997, 1295
RseI	CAYNNNNRTG	2 cut(s)	434, 1689
SalI	GTCGAC	1 cut(s)	365
SaqAI	TTAA	1 cut(s)	981
Sau3AI	GATC	7 cut(s)	453, 900, 963, 1231, 1341, 1630, 1693
Sau96I	GGNCC	9 cut(s)	4, 141, 142, 279, 565, 702, 732, 946, 1019
SchI	GAGTC	4 cut(s)	461, 480, 1108, 1517
ScrFI	CCNGG	4 cut(s)	277, 308, 386, 531
SduI	GDGCHC	8 cut(s)	145, 270, 288, 609, 650, 937, 1460, 1560
SfaNI	GCATC	3 cut(s)	28, 1218, 1408
Sfr274I	CTCGAG	1 cut(s)	872
SinI	GGWCC	3 cut(s)	565, 702, 732
SlaI	CTCGAG	1 cut(s)	872
SmiMI	CAYNNNNRTG	2 cut(s)	434, 1689
SmlI	CTYRAG	4 cut(s)	74, 643, 872, 1085
SmoI	CTYRAG	4 cut(s)	74, 643, 872, 1085
Sse9I	AATT	4 cut(s)	43, 374, 580, 660
SspI	AATATT	1 cut(s)	1363
SspMI	CTAG	1 cut(s)	974
StyD4I	CCNGG	4 cut(s)	275, 306, 384, 529
StyI	CCWWGG	3 cut(s)	270, 1179, 1236
TaaI	ACNGT	3 cut(s)	104, 158, 295
TaiI	ACGT	6 cut(s)	394, 743, 774, 1128, 1287, 1623
TaqI	TCGA	9 cut(s)	366, 456, 465, 484, 552, 873, 966, 1331, 1444
TasI	AATT	4 cut(s)	43, 374, 580, 660
TatI	WGTACW	2 cut(s)	621, 1294
TauI	GCSGC	5 cut(s)	762, 765, 973, 1533, 1546
TfiI	GAWTC	4 cut(s)	11, 406, 1679, 1702
Tru1I	TTAA	1 cut(s)	981
Tru9I	TTAA	1 cut(s)	981
TscAI	CASTG	4 cut(s)	481, 796, 931, 1375
TseFI	GTSAC	2 cut(s)	392, 829
Tsp45I	GTSAC	2 cut(s)	392, 829
TspDTI	ATGAA	5 cut(s)	32, 135, 707, 1229, 1636
TspGWI	ACGGA	2 cut(s)	532, 1278
TspRI	CASTG	4 cut(s)	481, 796, 931, 1375
Tth111I	GACNNNGTC	1 cut(s)	569
VpaK11BI	GGWCC	3 cut(s)	565, 702, 732
XagI	CCTNNNNNAGG	2 cut(s)	872, 1007
XceI	RCATGY	1 cut(s)	841
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1443, 1597
XhoI	CTCGAG	1 cut(s)	872
XmiI	GTMKAC	1 cut(s)	366
XmnI	GAANNNNTTC	1 cut(s)	1253
XspI	CTAG	1 cut(s)	974
ZraI	GACGTC	1 cut(s)	1285
Zsp2I	ATGCAT	1 cut(s)	258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.