Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003881

Physical Location & Seq

Genomic Coordinates Forward (+)

24612620 .. 24614805

Gene Structure 2186 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G66120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1719 bp

ATGGATAATCTAGTGTTATGTGAAGCAAACAATGTTCCTCTAACGCCCATAACGTTCTTGAAGAGAGCTTCGGAGTGTTATCCCAACCGAACTTCGATAATCTACGGGCAAACTCGCTTCACTTGGCCTCAGACTTATGACCGTTGCTGTCGTCTAGCTGCTTCTCTCCTCTCTCTTAACATCACCCGAAACGACGTTGTCTCGATTTTGGCCCCAAACGTACCTGCCATGTACGAGATGCACTTTAGCGTTCCCATGACCGGAGCCGTACTTAACCCCATTAACACCCGTCTTGACGCCAAAACCATAGCCATCATTCTCCGCCACGCCGAGCCAAAGATCTTATTTGTGGACTACGAGTTTGCTCCTTTGATCCAAGAAGTCCTCCGTCTCATACCCACTTACCAGTCACAACCGCATCCGCGGATCATATTAATTAACGAGATTGATTCCACTACAAAGCCTTTCTCCAAGGAGTTAGACTACGAGGGTCTCATCCGAAAAGGAGAACCTACGCCATCCTCCTCCGCAAGCATGTTCCGTGTCCACAACGAGCATGACCCTATCTCCTTGAACTACACGTCAGGTACCACGGCAGACCCAAAAGGTGTGGTGATTAGCCACCAAGGAGCCTACTTGAGCGCTTTGAGCTCCATTATTGGCTGGGAAATGGGGATTTTCCCTGTCTACCTCTGGACACTACCTATGTTTCATTGCAATGGATGGACGCACACGTGGTCCGTCGCTGCTCGTGGTGGTACTAATGTATGCATCAGACATGTCACTGCTCCAGAGATCTATAAGAACATAGAATTGCATGGCGTGACTCACATGTCATGTGTTCCTACGGTCTTTAGGTTTCTCCTTGAAGGAAGTCGAACCGACCAGTCACCCAAGTCCTCGCCGGTTCAGGTGTTGACTGGAGGTTCTTCTCCTCCGGCTGTCCTTATAAAGAAAGTTGAGCAGCTGGGGTTTCACGTGATGCATGGCTATGGGCTTACAGAGGCCACCGGCCCGGTTCTCTTTTGTGAATGGCAAGACGAGTGGAACAAACTACCAGAGCATCAACAGATAGAGCTACAACAAAGGCAAGGTGTAAGGAACCTAACCCTAGCCGACGTTGACGTGAAGAACACGAAAACTCTAGAGAGTGTCCCACGCGACGGGAAGACAATGGGAGAAATTGTTATTAAAGGAAGCAGCCTAATGAAAGGGTATCTAAAGAATCCTAAAGCAACATCTGAAGCATTTAAACACGGATGGCTTAACACGGGAGACATAGGTGTGATTCATCCTGATGGATATGTAGAGATCAAAGATCGATCCAAAGACATCATAATCTCGGGAGGTGAGAACATAAGTAGTATTGAGGTCGAGAAAGTTCTTTACATGTATCAGGAAGTGCTCGAGGCTGCGGTTGTAGCCATGCCTCATCCTCTATGGGGTGAAACCCCGTGTGCGTTTGTTGTTCTAAAGAAGGGTGAAGAGGGATTGGTGACTAGTGAAGGAGATCTCATTAAATATTGCCGTGAGAATATGCCGCATTTTATGTGTCCGAAAAAGGTGGTGTTCTTTCAAGAGCTTCCAAAGAATAGTAACGGAAAGATCCTAAAGTCTAAGTTGAGAGACATCGCTAAGGCATTGGTGGTCAGGGAGGACGATGCCGGCTCCAAGAAAGTTCATCAACGCAGTATCGAACACGTTAGTTCGAGGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0016143 GO:0016144 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018858 GO:0019605 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046459 GO:0047760 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

572

Amino Acids

63.94

Weight (kDa)

7.3

Isoelectric Point (pI)

42.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	21 - 407	2.7e-78	AMP-binding enzyme
AMP-binding_C	PF13193	457 - 535	8.8e-23	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65880 AT1G65890 AT1G66120
fragaria_vesca	FvH4_1g27440 FvH4_1g27450
malus_domestica	MD09G1280400.v1.1 MD17G1287600.v1.1
prunus_persica	Prupe.3G013700_v2.0.a1
pyrus_communis	pycom09g18850 pycom17g27060
rosa_chinensis	RchiOBHm_Chr3g0488561 RchiOBHm_Chr3g0488571
rosa_laevigata	RLG00000022942 RLG00000022943
rosa_multiflora	Rmu_sc0004402.1_g000006 Rmu_sc0004402.1_g000007 Rmu_sc0005363.1_g000024 Rmu_sc0005363.1_g000025
rosa_roxburghii	Rroxscaffold_6G00394130 Rroxscaffold_6G00394140
rosa_rugosa	Rorug03G0239300 Rorug03G0239400
rosa_samantha	Rh3AG288300 Rh3AG288400 Rh3BG325000 Rh3BG325100 Rh3CG322200 Rh3CG322300 Rh3DG321000 Rh3DG321100 Rh4AG012700 Rh7BG359000
rosa_wichuraiana	Rw3G025510 Rw3G025520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	950
AasI	GACNNNNNNGTC	1 cut(s)	832
Acc36I	ACCTGC	1 cut(s)	232
Acc65I	GGTACC	1 cut(s)	587
AccB1I	GGYRCC	1 cut(s)	587
AccI	GTMKAC	1 cut(s)	687
AccII	CGCG	2 cut(s)	424, 1161
AciI	CCGC	7 cut(s)	322, 416, 422, 424, 528, 1415, 1541
AclI	AACGTT	1 cut(s)	53
AclWI	GGATC	4 cut(s)	367, 434, 1317, 1600
AcuI	CTGAAG	1 cut(s)	1263
AcvI	CACGTG	2 cut(s)	735, 979
AcyI	GRCGYC	1 cut(s)	297
AfaI	GTAC	5 cut(s)	222, 233, 270, 589, 760
AfeI	AGCGCT	1 cut(s)	643
AfiI	CCNNNNNNNGG	3 cut(s)	260, 1163, 1442
AflIII	ACRYGT	6 cut(s)	579, 732, 778, 831, 1389, 1699
AgsI	TTSAA	4 cut(s)	61, 574, 869, 1577
AhlI	ACTAGT	1 cut(s)	1499
AjiI	CACGTC	2 cut(s)	582, 1126
AluBI	AGCT	6 cut(s)	68, 158, 651, 967, 1078, 1582
AluI	AGCT	6 cut(s)	68, 158, 651, 967, 1078, 1582
Alw21I	GWGCWC	2 cut(s)	653, 1407
Alw26I	GTCTC	5 cut(s)	205, 395, 497, 1269, 1620
AlwI	GGATC	4 cut(s)	367, 434, 1317, 1600
Ama87I	CYCGRG	2 cut(s)	1342, 1406
Aor51HI	AGCGCT	1 cut(s)	643
AoxI	GGCC	4 cut(s)	125, 210, 1005, 1012
ApeKI	GCWGC	5 cut(s)	158, 746, 964, 1200, 1412
ArsI	GACNNNNNNTTYG	2 cut(s)	344, 376
AseI	ATTAAT	1 cut(s)	434
Asp718I	GGTACC	1 cut(s)	587
AspLEI	GCGC	1 cut(s)	644
AspS9I	GGNCC	3 cut(s)	211, 738, 1013
AsuC2I	CCSGG	1 cut(s)	1016
AsuHPI	GGTGA	7 cut(s)	175, 625, 882, 1361, 1457, 1493, 1507
AvaI	CYCGRG	2 cut(s)	1342, 1406
AvaII	GGWCC	1 cut(s)	738
BaeI	ACNNNNGTAYC	2 cut(s)	750, 783
BanI	GGYRCC	1 cut(s)	587
BanII	GRGCYC	1 cut(s)	653
BauI	CACGAG	1 cut(s)	750
BbrPI	CACGTG	2 cut(s)	735, 979
BbsI	GAAGAC	1 cut(s)	1175
Bbv12I	GWGCWC	2 cut(s)	653, 1407
BbvI	GCAGC	5 cut(s)	145, 733, 976, 1212, 1399
BccI	CCATC	5 cut(s)	320, 526, 717, 1254, 1292
BceAI	ACGGC	3 cut(s)	251, 609, 1512
BcnI	CCSGG	1 cut(s)	1016
BcoDI	GTCTC	5 cut(s)	205, 395, 497, 1269, 1620
BcuI	ACTAGT	1 cut(s)	1499
BfaI	CTAG	5 cut(s)	11, 155, 1112, 1145, 1500
BfoI	RGCGCY	1 cut(s)	645
BfuAI	ACCTGC	1 cut(s)	232
BglII	AGATCT	3 cut(s)	339, 795, 1510
BisI	GCNGC	6 cut(s)	159, 747, 965, 1201, 1413, 1541
BlsI	GCNGC	6 cut(s)	160, 748, 966, 1202, 1414, 1542
Bme1390I	CCNGG	1 cut(s)	1016
Bme18I	GGWCC	1 cut(s)	738
BmeT110I	CYCGRG	2 cut(s)	1342, 1406
BmgBI	CACGTC	2 cut(s)	582, 1126
BmgT120I	GGNCC	3 cut(s)	211, 738, 1013
BmiI	GGNNCC	6 cut(s)	213, 265, 589, 631, 1103, 1669
BmrFI	CCNGG	1 cut(s)	1016
BmsI	GCATC	6 cut(s)	228, 427, 780, 972, 1072, 1651
BpiI	GAAGAC	1 cut(s)	1175
BpmI	CTGGAG	2 cut(s)	774, 942
Bpu10I	CCTNAGC	1 cut(s)	1635
BpuEI	CTTGAG	1 cut(s)	658
BpuMI	CCSGG	1 cut(s)	1016
Bsa29I	ATCGAT	1 cut(s)	1321
BsaAI	YACGTR	2 cut(s)	735, 979
BsaHI	GRCGYC	1 cut(s)	297
BsaI	GGTCTC	1 cut(s)	497
BsaJI	CCNNGG	4 cut(s)	422, 471, 591, 625
BsaWI	WCCGGW	1 cut(s)	260
BsaXI	ACNNNNNCTCC	4 cut(s)	551, 581, 1170, 1200
Bsc4I	CCNNNNNNNGG	3 cut(s)	260, 1163, 1442
Bse118I	RCCGGY	3 cut(s)	904, 1010, 1664
Bse1I	ACTGG	3 cut(s)	406, 886, 925
Bse3DI	GCAATG	2 cut(s)	712, 724
BseCI	ATCGAT	1 cut(s)	1321
BseDI	CCNNGG	4 cut(s)	422, 471, 591, 625
BseGI	GGATG	7 cut(s)	418, 495, 518, 728, 1265, 1291, 1432
BseLI	CCNNNNNNNGG	3 cut(s)	260, 1163, 1442
BseMI	GCAATG	2 cut(s)	712, 724
BseMII	CTCAG	1 cut(s)	143
BseNI	ACTGG	3 cut(s)	406, 886, 925
BseRI	GAGGAG	3 cut(s)	158, 514, 924
BseXI	GCAGC	5 cut(s)	145, 733, 976, 1212, 1399
BseYI	CCCAGC	2 cut(s)	663, 967
Bsh1236I	CGCG	2 cut(s)	424, 1161
BshFI	GGCC	4 cut(s)	127, 212, 1007, 1014
BshNI	GGYRCC	1 cut(s)	587
BshVI	ATCGAT	1 cut(s)	1321
BsiHKAI	GWGCWC	2 cut(s)	653, 1407
BsiHKCI	CYCGRG	2 cut(s)	1342, 1406
BsiSI	CCGG	6 cut(s)	261, 905, 938, 1011, 1016, 1665
BslFI	GGGAC	1 cut(s)	1139
BslI	CCNNNNNNNGG	3 cut(s)	260, 1163, 1442
BsmAI	GTCTC	5 cut(s)	205, 395, 497, 1269, 1620
BsmBI	CGTCTC	1 cut(s)	395
BsmFI	GGGAC	1 cut(s)	1139
BsnI	GGCC	4 cut(s)	127, 212, 1007, 1014
Bso31I	GGTCTC	1 cut(s)	497
BsoBI	CYCGRG	2 cut(s)	1342, 1406
Bsp1286I	GDGCHC	2 cut(s)	653, 1407
Bsp143I	GATC	9 cut(s)	339, 372, 426, 795, 1311, 1318, 1322, 1510, 1605
BspACI	CCGC	7 cut(s)	322, 416, 422, 424, 528, 1415, 1541
BspANI	GGCC	4 cut(s)	127, 212, 1007, 1014
BspCNI	CTCAG	1 cut(s)	142
BspDI	ATCGAT	1 cut(s)	1321
BspFNI	CGCG	2 cut(s)	424, 1161
BspLI	GGNNCC	6 cut(s)	213, 265, 589, 631, 1103, 1669
BspMI	ACCTGC	1 cut(s)	232
BspPI	GGATC	4 cut(s)	367, 434, 1317, 1600
BspT107I	GGYRCC	1 cut(s)	587
BspTNI	GGTCTC	1 cut(s)	497
BsrDI	GCAATG	2 cut(s)	712, 724
BsrFI	RCCGGY	3 cut(s)	904, 1010, 1664
BsrI	ACTGG	3 cut(s)	406, 886, 925
BssAI	RCCGGY	3 cut(s)	904, 1010, 1664
BssECI	CCNNGG	4 cut(s)	422, 471, 591, 625
BssMI	GATC	9 cut(s)	339, 372, 426, 795, 1311, 1318, 1322, 1510, 1605
BssNI	GRCGYC	1 cut(s)	297
BssSI	CACGAG	1 cut(s)	750
BssT1I	CCWWGG	2 cut(s)	471, 625
Bst2BI	CACGAG	1 cut(s)	750
Bst4CI	ACNGT	2 cut(s)	143, 850
Bst6I	CTCTTC	2 cut(s)	56, 1479
BstACI	GRCGYC	1 cut(s)	297
BstBAI	YACGTR	2 cut(s)	735, 979
BstC8I	GCNNGC	2 cut(s)	532, 1666
BstDEI	CTNAG	3 cut(s)	129, 1617, 1635
BstDSI	CCRYGG	2 cut(s)	422, 591
BstF5I	GGATG	7 cut(s)	418, 495, 518, 728, 1265, 1291, 1432
BstFNI	CGCG	2 cut(s)	424, 1161
BstH2I	RGCGCY	1 cut(s)	645
BstHHI	GCGC	1 cut(s)	644
BstKTI	GATC	9 cut(s)	342, 375, 429, 798, 1314, 1321, 1325, 1513, 1608
BstMAI	GTCTC	5 cut(s)	205, 395, 497, 1269, 1620
BstMBI	GATC	9 cut(s)	339, 372, 426, 795, 1311, 1318, 1322, 1510, 1605
BstMWI	GCNNNNNNNGC	2 cut(s)	648, 1421
BstNSI	RCATGY	4 cut(s)	538, 782, 835, 1393
BstSCI	CCNGG	1 cut(s)	1014
BstUI	CGCG	2 cut(s)	424, 1161
BstV1I	GCAGC	5 cut(s)	145, 733, 976, 1212, 1399
BstV2I	GAAGAC	1 cut(s)	1175
BstX2I	RGATCY	4 cut(s)	339, 795, 1510, 1605
BstYI	RGATCY	4 cut(s)	339, 795, 1510, 1605
Bsu15I	ATCGAT	1 cut(s)	1321
BsuRI	GGCC	4 cut(s)	127, 212, 1007, 1014
BsuTUI	ATCGAT	1 cut(s)	1321
BtgI	CCRYGG	2 cut(s)	422, 591
BtgZI	GCGATG	1 cut(s)	1615
BtrI	CACGTC	2 cut(s)	582, 1126
BtsCI	GGATG	7 cut(s)	418, 495, 518, 728, 1265, 1291, 1432
BtsI	GCAGTG	1 cut(s)	783
BtsIMutI	CAGTG	1 cut(s)	783
BveI	ACCTGC	1 cut(s)	232
Cac8I	GCNNGC	2 cut(s)	532, 1666
CfoI	GCGC	1 cut(s)	644
Cfr10I	RCCGGY	3 cut(s)	904, 1010, 1664
Cfr13I	GGNCC	3 cut(s)	211, 738, 1013
Cfr42I	CCGCGG	1 cut(s)	425
ClaI	ATCGAT	1 cut(s)	1321
CseI	GACGC	2 cut(s)	305, 736
Csp6I	GTAC	5 cut(s)	221, 232, 269, 588, 759
CspCI	CAANNNNNGTGG	4 cut(s)	591, 626, 1025, 1060
CviQI	GTAC	5 cut(s)	221, 232, 269, 588, 759
DdeI	CTNAG	3 cut(s)	129, 1617, 1635
DpnI	GATC	9 cut(s)	341, 374, 428, 797, 1313, 1320, 1324, 1512, 1607
DpnII	GATC	9 cut(s)	339, 372, 426, 795, 1311, 1318, 1322, 1510, 1605
DraI	TTTAAA	1 cut(s)	1252
DrdI	GACNNNNNNGTC	1 cut(s)	832
DseDI	GACNNNNNNGTC	1 cut(s)	832
Eam1104I	CTCTTC	2 cut(s)	56, 1479
EarI	CTCTTC	2 cut(s)	56, 1479
EciI	GGCGGA	1 cut(s)	311
Ecl136II	GAGCTC	1 cut(s)	651
Eco130I	CCWWGG	2 cut(s)	471, 625
Eco24I	GRGCYC	1 cut(s)	653
Eco31I	GGTCTC	1 cut(s)	497
Eco47I	GGWCC	1 cut(s)	738
Eco47III	AGCGCT	1 cut(s)	643
Eco53kI	GAGCTC	1 cut(s)	651
Eco57I	CTGAAG	1 cut(s)	1263
Eco72I	CACGTG	2 cut(s)	735, 979
Eco88I	CYCGRG	2 cut(s)	1342, 1406
EcoICRI	GAGCTC	1 cut(s)	651
EcoT14I	CCWWGG	2 cut(s)	471, 625
EcoT22I	ATGCAT	2 cut(s)	773, 987
EcoT38I	GRGCYC	1 cut(s)	653
ErhI	CCWWGG	2 cut(s)	471, 625
Esp3I	CGTCTC	1 cut(s)	395
FaqI	GGGAC	1 cut(s)	1139
FblI	GTMKAC	1 cut(s)	687
Fnu4HI	GCNGC	6 cut(s)	159, 747, 965, 1201, 1413, 1541
FokI	GGATG	7 cut(s)	405, 482, 505, 735, 1272, 1278, 1419
FriOI	GRGCYC	1 cut(s)	653
Fsp4HI	GCNGC	6 cut(s)	159, 747, 965, 1201, 1413, 1541
FspBI	CTAG	5 cut(s)	11, 155, 1112, 1145, 1500
GlaI	GCGC	1 cut(s)	643
GluI	GCNGC	6 cut(s)	159, 747, 965, 1201, 1413, 1541
GsaI	CCCAGC	2 cut(s)	667, 971
GsuI	CTGGAG	2 cut(s)	774, 942
HaeII	RGCGCY	1 cut(s)	645
HaeIII	GGCC	4 cut(s)	127, 212, 1007, 1014
HapII	CCGG	6 cut(s)	261, 905, 938, 1011, 1016, 1665
HgaI	GACGC	2 cut(s)	305, 736
HhaI	GCGC	1 cut(s)	644
Hin1I	GRCGYC	1 cut(s)	297
Hin6I	GCGC	1 cut(s)	642
HinP1I	GCGC	1 cut(s)	642
HincII	GTYRAC	2 cut(s)	918, 1123
HindII	GTYRAC	2 cut(s)	918, 1123
HinfI	GANTC	4 cut(s)	449, 826, 1225, 1288
HpaII	CCGG	6 cut(s)	261, 905, 938, 1011, 1016, 1665
HphI	GGTGA	7 cut(s)	175, 625, 882, 1361, 1457, 1493, 1507
Hpy166II	GTNNAC	5 cut(s)	352, 547, 688, 918, 1123
Hpy188I	TCNGA	6 cut(s)	73, 132, 500, 776, 1243, 1557
Hpy8I	GTNNAC	5 cut(s)	352, 547, 688, 918, 1123
Hpy99I	CGWCG	4 cut(s)	197, 746, 1121, 1166
HpyAV	CCTTC	3 cut(s)	863, 1471, 1499
HpyCH4III	ACNGT	2 cut(s)	143, 850
HpyCH4IV	ACGT	9 cut(s)	53, 195, 219, 581, 734, 978, 1119, 1125, 1701
HpyCH4V	TGCA	5 cut(s)	241, 717, 771, 817, 985
HpyF10VI	GCNNNNNNNGC	2 cut(s)	648, 1421
HpyF3I	CTNAG	3 cut(s)	129, 1617, 1635
HpySE526I	ACGT	9 cut(s)	53, 195, 219, 581, 734, 978, 1119, 1125, 1701
Hsp92I	GRCGYC	1 cut(s)	297
HspAI	GCGC	1 cut(s)	642
KpnI	GGTACC	1 cut(s)	591
KroI	GCCGGC	1 cut(s)	1664
KroNI	GCCGGC	1 cut(s)	1666
KspI	CCGCGG	1 cut(s)	425
Kzo9I	GATC	9 cut(s)	339, 372, 426, 795, 1311, 1318, 1322, 1510, 1605
LmnI	GCTCC	6 cut(s)	263, 370, 629, 656, 793, 1673
Lsp1109I	GCAGC	5 cut(s)	145, 733, 976, 1212, 1399
LweI	GCATC	6 cut(s)	228, 427, 780, 972, 1072, 1651
MaeI	CTAG	5 cut(s)	11, 155, 1112, 1145, 1500
MaeII	ACGT	9 cut(s)	53, 195, 219, 581, 734, 978, 1119, 1125, 1701
MaeIII	GTNAC	6 cut(s)	408, 781, 823, 888, 1495, 1595
MalI	GATC	9 cut(s)	341, 374, 428, 797, 1313, 1320, 1324, 1512, 1607
MboI	GATC	9 cut(s)	339, 372, 426, 795, 1311, 1318, 1322, 1510, 1605
MboII	GAAGA	5 cut(s)	73, 921, 1141, 1180, 1496
MflI	RGATCY	4 cut(s)	339, 795, 1510, 1605
MhlI	GDGCHC	2 cut(s)	653, 1407
MluCI	AATT	3 cut(s)	435, 812, 1182
MlyI	GAGTC	1 cut(s)	820
Mph1103I	ATGCAT	2 cut(s)	773, 987
MroNI	GCCGGC	1 cut(s)	1664
MseI	TTAA	9 cut(s)	177, 273, 282, 434, 438, 1191, 1251, 1266, 1518
MslI	CAYNNNNRTG	4 cut(s)	717, 990, 1283, 1296
MspA1I	CMGCKG	2 cut(s)	424, 967
MspI	CCGG	6 cut(s)	261, 905, 938, 1011, 1016, 1665
MspR9I	CCNGG	1 cut(s)	1016
MvnI	CGCG	2 cut(s)	424, 1161
MwoI	GCNNNNNNNGC	2 cut(s)	648, 1421
NaeI	GCCGGC	1 cut(s)	1666
NciI	CCSGG	1 cut(s)	1016
NdeII	GATC	9 cut(s)	339, 372, 426, 795, 1311, 1318, 1322, 1510, 1605
NgoMIV	GCCGGC	1 cut(s)	1664
NlaIV	GGNNCC	6 cut(s)	213, 265, 589, 631, 1103, 1669
NmeAIII	GCCGAG	1 cut(s)	355
NmuCI	GTSAC	5 cut(s)	408, 781, 823, 888, 1495
NsiI	ATGCAT	2 cut(s)	773, 987
NspI	RCATGY	4 cut(s)	538, 782, 835, 1393
PacI	TTAATTAA	1 cut(s)	438
PaeR7I	CTCGAG	1 cut(s)	1406
PciI	ACATGT	3 cut(s)	778, 831, 1389
PcsI	WCGNNNNNNNCGW	1 cut(s)	50
PdiI	GCCGGC	1 cut(s)	1666
PfeI	GAWTC	3 cut(s)	449, 1225, 1288
PflFI	GACNNNGTC	1 cut(s)	197
PkrI	GCNGC	6 cut(s)	160, 748, 966, 1202, 1414, 1542
PleI	GAGTC	1 cut(s)	820
PmaCI	CACGTG	2 cut(s)	735, 979
PmlI	CACGTG	2 cut(s)	735, 979
PpsI	GAGTC	1 cut(s)	820
Ppu21I	YACGTR	2 cut(s)	735, 979
PscI	ACATGT	3 cut(s)	778, 831, 1389
PshBI	ATTAAT	1 cut(s)	434
PsiI	TTATAA	1 cut(s)	950
Psp124BI	GAGCTC	1 cut(s)	653
Psp1406I	AACGTT	1 cut(s)	53
PspCI	CACGTG	2 cut(s)	735, 979
PspFI	CCCAGC	2 cut(s)	663, 967
PspN4I	GGNNCC	6 cut(s)	213, 265, 589, 631, 1103, 1669
PspPI	GGNCC	3 cut(s)	211, 738, 1013
PspXI	VCTCGAGB	1 cut(s)	1406
PsuI	RGATCY	4 cut(s)	339, 795, 1510, 1605
PsyI	GACNNNGTC	1 cut(s)	197
PvuII	CAGCTG	1 cut(s)	967
RsaI	GTAC	5 cut(s)	222, 233, 270, 589, 760
RsaNI	GTAC	5 cut(s)	221, 232, 269, 588, 759
RseI	CAYNNNNRTG	4 cut(s)	717, 990, 1283, 1296
SacI	GAGCTC	1 cut(s)	653
SacII	CCGCGG	1 cut(s)	425
SaqAI	TTAA	9 cut(s)	177, 273, 282, 434, 438, 1191, 1251, 1266, 1518
SatI	GCNGC	6 cut(s)	159, 747, 965, 1201, 1413, 1541
Sau3AI	GATC	9 cut(s)	339, 372, 426, 795, 1311, 1318, 1322, 1510, 1605
Sau96I	GGNCC	3 cut(s)	211, 738, 1013
SchI	GAGTC	1 cut(s)	820
ScrFI	CCNGG	1 cut(s)	1016
SduI	GDGCHC	2 cut(s)	653, 1407
SfaNI	GCATC	6 cut(s)	228, 427, 780, 972, 1072, 1651
Sfr274I	CTCGAG	1 cut(s)	1406
Sfr303I	CCGCGG	1 cut(s)	425
SgrBI	CCGCGG	1 cut(s)	425
SinI	GGWCC	1 cut(s)	738
SlaI	CTCGAG	1 cut(s)	1406
SmiMI	CAYNNNNRTG	4 cut(s)	717, 990, 1283, 1296
SmlI	CTYRAG	2 cut(s)	637, 1406
SmoI	CTYRAG	2 cut(s)	637, 1406
SpeI	ACTAGT	1 cut(s)	1499
Sse9I	AATT	3 cut(s)	435, 812, 1182
SsiI	CCGC	7 cut(s)	322, 416, 422, 424, 528, 1415, 1541
SspI	AATATT	1 cut(s)	1523
SspMI	CTAG	5 cut(s)	11, 155, 1112, 1145, 1500
SstI	GAGCTC	1 cut(s)	653
StyD4I	CCNGG	1 cut(s)	1014
StyI	CCWWGG	2 cut(s)	471, 625
TaaI	ACNGT	2 cut(s)	143, 850
TaiI	ACGT	9 cut(s)	56, 198, 222, 584, 737, 981, 1122, 1128, 1704
TaqI	TCGA	8 cut(s)	95, 203, 877, 1321, 1374, 1407, 1695, 1709
TasI	AATT	3 cut(s)	435, 812, 1182
TauI	GCSGC	1 cut(s)	1543
TfiI	GAWTC	3 cut(s)	449, 1225, 1288
Tru1I	TTAA	9 cut(s)	177, 273, 282, 434, 438, 1191, 1251, 1266, 1518
Tru9I	TTAA	9 cut(s)	177, 273, 282, 434, 438, 1191, 1251, 1266, 1518
TscAI	CASTG	1 cut(s)	790
TseFI	GTSAC	5 cut(s)	408, 781, 823, 888, 1495
TseI	GCWGC	5 cut(s)	158, 746, 964, 1200, 1412
Tsp45I	GTSAC	5 cut(s)	408, 781, 823, 888, 1495
TspDTI	ATGAA	4 cut(s)	701, 1223, 1280, 1670
TspGWI	ACGGA	5 cut(s)	377, 530, 730, 1272, 1614
TspRI	CASTG	1 cut(s)	790
Tth111I	GACNNNGTC	1 cut(s)	197
VpaK11BI	GGWCC	1 cut(s)	738
VspI	ATTAAT	1 cut(s)	434
XbaI	TCTAGA	1 cut(s)	1144
XceI	RCATGY	4 cut(s)	538, 782, 835, 1393
XhoI	CTCGAG	1 cut(s)	1406
XmiI	GTMKAC	1 cut(s)	687
XspI	CTAG	5 cut(s)	11, 155, 1112, 1145, 1500
Zsp2I	ATGCAT	2 cut(s)	773, 987

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.