Rroxscaffold_6G00394140

butyrate--CoA ligase AAE11, peroxisomal-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003881
Physical Location & Seq
Reverse (-)
14490853 .. 14492920
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394140.1

Sequence Viewer

Length: 1338 bp
ATGGGTACGGAGATGAATATGCTTCCCAAATGTGAAGCCAATTACGCTGCTCTCACCCCAACGACTTTCTTGAAGAGAGCCGCGGCGTTTTATGCTAACCGCACCTCCCTCATCTACGAAGGCACTCGCTTCACTTGGCGCCAAACCTACGACCGCTGCTCCCGTCTCGCCTCCTCTCTTGTTTGTCTCAACATTGTCAAGCATGATGTTGTATCAGTCCTGGCTCCTAATGTTCCGGCAACGTATGAGATGCACTTTGCGGTGCCCATGGCTGGTGCGGTGCTCAACACCATCAACACCCGCCTTGATGCGAAGAACATAGCTTCCATTCTCAAACACTCAGGCGCCAAGGTCTTCTTTGTGGACTGCCAGTTCGTCCCGGTGGGGCGTGAGGCTCTCAAAATCCTTATGAACAATTCCGAGCCCTCCATGATGCCGTTGGTCATCGTGATTGATGACATCGACTCACCCACAGGTGTTCGATTGGGCGAACTGGAGTATGAACAACTCATCCACAAGGGCAATCCCGGGTTTACCTCAGTCGAGCTTGACAACGAGTGGGACCCAATCGCTTTGAATTACACTTCTGGCACGACGTCTGAACCCAAAGGCGTTGTGTACAGCCACAGAGGTGCTTACTTGAGCACTTTAAGCCTAATCCTGGGTTGGGAAATGGGGAGCGAGCCTGTTTATCTATGGACGCTCCCTATGTTCCACTGCAACGGCTGGACCTTCACTTGGGGTGTGGCGGCACGCGGCGGAACCAACGTCTGCCTCCGAAACACCACCGCGTACGACATCTACCGCAACATCAAACGACACCAGGTCACCCACATGTGCTGTGCTCCCATCATCTTCAACATTCTCCTCGAGGCCAAACCCCACGAGCGCTGCGAGATCGTGTCCCCTATTCAGATACTTACCGGTGGCGCACCACCCCCAGCTCCTTTGCTGGAGAAAATCGAGCCGTTGGGTTTCAAGGTGACACATGCATATGGCCTGACTGAAGCAACCGGACCGGCTTTGGTTTGCGAGTGGCAGGCCAAGTGGAACAAGCTTCCCAAGGAGGAACAATCAAATCTTAAAGCAAGGCAAGGAATAAGCATTTTGACTCTGGCTGATGTTGACGTGAAGGACTCGGAAACAATGGAGAGTGTTCCTCACGACGGCAAAACCATGGGGGAGATTGTGCTGCGTGGGAGTAGCATCATGAAGGGTTACTTCAAGGACCCAAAGGCCACCAACAAAGCGTTCAAGAACGGCTGGTTCTTCACCGGCGATGTCGGTGTTATCCACCAGATGGTTATTTGGAAATCAAAGACAGATCGAAAGACGTGA

Protein Analysis

445

Amino Acids

49.48

Weight (kDa)

7.97

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 25 - 408 2e-82 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 599
AbsI CCTCGAGG 1 cut(s) 869
AccB1I GGYRCC 3 cut(s) 138, 262, 344
AccB7I CCANNNNNTGG 1 cut(s) 1300
AccII CGCG 3 cut(s) 83, 756, 791
AcuI CTGAAG 1 cut(s) 1026
AcyI GRCGYC 3 cut(s) 139, 345, 596
AfaI GTAC 3 cut(s) 7, 620, 794
AfeI AGCGCT 1 cut(s) 890
AfiI CCNNNNNNNGG 6 cut(s) 272, 661, 667, 738, 1166, 1300
AflIII ACRYGT 1 cut(s) 834
AgeI ACCGGT 1 cut(s) 923
AgsI TTSAA 6 cut(s) 73, 577, 859, 979, 1225, 1255
AhdI GACNNNNNGTC 1 cut(s) 824
AjiI CACGTC 2 cut(s) 1129, 1335
AjnI CCWGG 3 cut(s) 219, 660, 822
AjuI GAANNNNNNNTTGG 2 cut(s) 1235, 1267
AleI CACNNNNGTG 1 cut(s) 630
AloI GAACNNNNNNTCC 6 cut(s) 308, 340, 356, 388, 495, 527
AluBI AGCT 4 cut(s) 323, 547, 944, 1057
AluI AGCT 4 cut(s) 323, 547, 944, 1057
Alw21I GWGCWC 3 cut(s) 285, 647, 847
Alw26I GTCTC 2 cut(s) 170, 191
Ama87I CYCGRG 2 cut(s) 527, 869
Aor51HI AGCGCT 1 cut(s) 890
AoxI GGCC 4 cut(s) 873, 997, 1041, 1236
ApeKI GCWGC 4 cut(s) 47, 156, 891, 1192
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AsiGI ACCGGT 1 cut(s) 923
AspLEI GCGC 4 cut(s) 141, 347, 891, 932
AspS9I GGNCC 4 cut(s) 562, 729, 1016, 1228
AsuC2I CCSGG 3 cut(s) 380, 528, 529
AsuHPI GGTGA 5 cut(s) 46, 459, 820, 994, 1264
AvaI CYCGRG 2 cut(s) 527, 869
AvaII GGWCC 4 cut(s) 562, 729, 1016, 1228
BaeGI GKGCMC 1 cut(s) 267
BanI GGYRCC 3 cut(s) 138, 262, 344
BanII GRGCYC 1 cut(s) 426
BauI CACGAG 1 cut(s) 884
BbsI GAAGAC 1 cut(s) 346
Bbv12I GWGCWC 3 cut(s) 285, 647, 847
BbvI GCAGC 4 cut(s) 34, 143, 878, 1179
BccI CCATC 3 cut(s) 299, 857, 1294
BceAI ACGGC 5 cut(s) 421, 739, 952, 1183, 1276
BciT130I CCWGG 3 cut(s) 221, 662, 824
BcnI CCSGG 3 cut(s) 380, 528, 529
BcoDI GTCTC 2 cut(s) 170, 191
BfoI RGCGCY 3 cut(s) 142, 348, 892
BisI GCNGC 8 cut(s) 48, 81, 84, 157, 750, 757, 892, 1193
BlsI GCNGC 8 cut(s) 49, 82, 85, 158, 751, 758, 893, 1194
Bme1390I CCNGG 6 cut(s) 221, 380, 528, 529, 662, 824
Bme18I GGWCC 4 cut(s) 562, 729, 1016, 1228
BmeRI GACNNNNNGTC 1 cut(s) 824
BmeT110I CYCGRG 2 cut(s) 527, 869
BmgBI CACGTC 2 cut(s) 1129, 1335
BmgT120I GGNCC 4 cut(s) 562, 729, 1016, 1228
BmiI GGNNCC 8 cut(s) 140, 225, 264, 346, 563, 564, 763, 1230
BmrFI CCNGG 6 cut(s) 221, 380, 528, 529, 662, 824
BmsI GCATC 4 cut(s) 240, 298, 423, 1215
BpiI GAAGAC 1 cut(s) 346
BplI GAGNNNNNCTC 2 cut(s) 1144, 1176
BpmI CTGGAG 2 cut(s) 515, 974
BpuEI CTTGAG 1 cut(s) 661
BpuMI CCSGG 3 cut(s) 380, 528, 529
BsaHI GRCGYC 3 cut(s) 139, 345, 596
BsaJI CCNNGG 7 cut(s) 81, 267, 348, 527, 661, 1062, 1176
BsaWI WCCGGW 2 cut(s) 923, 1013
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 143, 173
Bsc4I CCNNNNNNNGG 6 cut(s) 272, 661, 667, 738, 1166, 1300
Bse118I RCCGGY 3 cut(s) 923, 1018, 1274
Bse1I ACTGG 2 cut(s) 370, 498
BseBI CCWGG 3 cut(s) 221, 662, 824
BseDI CCNNGG 7 cut(s) 81, 267, 348, 527, 661, 1062, 1176
BseGI GGATG 1 cut(s) 510
BseLI CCNNNNNNNGG 6 cut(s) 272, 661, 667, 738, 1166, 1300
BseMII CTCAG 2 cut(s) 354, 552
BseNI ACTGG 2 cut(s) 370, 498
BseRI GAGGAG 2 cut(s) 163, 857
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 4 cut(s) 34, 143, 878, 1179
BseYI CCCAGC 1 cut(s) 940
Bsh1236I CGCG 3 cut(s) 83, 756, 791
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 4 cut(s) 875, 999, 1043, 1238
BshNI GGYRCC 3 cut(s) 138, 262, 344
BshTI ACCGGT 1 cut(s) 923
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 3 cut(s) 285, 647, 847
BsiHKCI CYCGRG 2 cut(s) 527, 869
BsiSI CCGG 7 cut(s) 236, 380, 528, 924, 1014, 1019, 1275
BsiWI CGTACG 1 cut(s) 792
BslFI GGGAC 3 cut(s) 362, 575, 889
BslI CCNNNNNNNGG 6 cut(s) 272, 661, 667, 738, 1166, 1300
BsmAI GTCTC 2 cut(s) 170, 191
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 3 cut(s) 362, 575, 889
BsnI GGCC 4 cut(s) 875, 999, 1043, 1238
BsoBI CYCGRG 2 cut(s) 527, 869
Bsp1286I GDGCHC 5 cut(s) 267, 285, 426, 647, 847
Bsp1407I TGTACA 1 cut(s) 618
Bsp143I GATC 2 cut(s) 897, 1324
Bsp19I CCATGG 2 cut(s) 267, 1176
BspANI GGCC 4 cut(s) 875, 999, 1043, 1238
BspCNI CTCAG 2 cut(s) 353, 551
BspFNI CGCG 3 cut(s) 83, 756, 791
BspHI TCATGA 1 cut(s) 1209
BspLI GGNNCC 8 cut(s) 140, 225, 264, 346, 563, 564, 763, 1230
BspT107I GGYRCC 3 cut(s) 138, 262, 344
BsrFI RCCGGY 3 cut(s) 923, 1018, 1274
BsrGI TGTACA 1 cut(s) 618
BsrI ACTGG 2 cut(s) 370, 498
BssAI RCCGGY 3 cut(s) 923, 1018, 1274
BssECI CCNNGG 7 cut(s) 81, 267, 348, 527, 661, 1062, 1176
BssMI GATC 2 cut(s) 897, 1324
BssNI GRCGYC 3 cut(s) 139, 345, 596
BssSI CACGAG 1 cut(s) 884
BssT1I CCWWGG 4 cut(s) 267, 348, 1062, 1176
Bst2BI CACGAG 1 cut(s) 884
Bst2UI CCWGG 3 cut(s) 221, 662, 824
Bst6I CTCTTC 1 cut(s) 68
BstACI GRCGYC 3 cut(s) 139, 345, 596
BstAUI TGTACA 1 cut(s) 618
BstC8I GCNNGC 3 cut(s) 683, 754, 1041
BstDEI CTNAG 2 cut(s) 340, 538
BstDSI CCRYGG 3 cut(s) 81, 267, 1176
BstEII GGTNACC 1 cut(s) 826
BstF5I GGATG 1 cut(s) 510
BstFNI CGCG 3 cut(s) 83, 756, 791
BstH2I RGCGCY 3 cut(s) 142, 348, 892
BstHHI GCGC 4 cut(s) 141, 347, 891, 932
BstKTI GATC 2 cut(s) 900, 1327
BstMAI GTCTC 2 cut(s) 170, 191
BstMBI GATC 2 cut(s) 897, 1324
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 3 cut(s) 44, 92, 651
BstNI CCWGG 3 cut(s) 221, 662, 824
BstNSI RCATGY 2 cut(s) 838, 992
BstPI GGTNACC 1 cut(s) 826
BstSCI CCNGG 6 cut(s) 219, 378, 526, 527, 660, 822
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 3 cut(s) 83, 756, 791
BstV1I GCAGC 4 cut(s) 34, 143, 878, 1179
BstV2I GAAGAC 1 cut(s) 346
BsuRI GGCC 4 cut(s) 875, 999, 1043, 1238
BtgI CCRYGG 3 cut(s) 81, 267, 1176
BtgZI GCGATG 1 cut(s) 1293
BtrI CACGTC 2 cut(s) 1129, 1335
BtsCI GGATG 1 cut(s) 510
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 1 cut(s) 715
Cac8I GCNNGC 3 cut(s) 683, 754, 1041
CciI TCATGA 1 cut(s) 1209
CfoI GCGC 4 cut(s) 141, 347, 891, 932
Cfr10I RCCGGY 3 cut(s) 923, 1018, 1274
Cfr13I GGNCC 4 cut(s) 562, 729, 1016, 1228
Cfr42I CCGCGG 1 cut(s) 84
Cfr9I CCCGGG 1 cut(s) 527
CpoI CGGWCCG 1 cut(s) 1016
CseI GACGC 1 cut(s) 709
CsiI ACCWGGT 1 cut(s) 822
Csp6I GTAC 3 cut(s) 6, 619, 793
CspAI ACCGGT 1 cut(s) 923
CspI CGGWCCG 1 cut(s) 1016
CviAII CATG 7 cut(s) 203, 268, 430, 835, 989, 1177, 1210
CviQI GTAC 3 cut(s) 6, 619, 793
DdeI CTNAG 2 cut(s) 340, 538
DinI GGCGCC 2 cut(s) 140, 346
DpnI GATC 2 cut(s) 899, 1326
DpnII GATC 2 cut(s) 897, 1324
DriI GACNNNNNGTC 1 cut(s) 824
Eam1104I CTCTTC 1 cut(s) 68
Eam1105I GACNNNNNGTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 774
Eco130I CCWWGG 4 cut(s) 267, 348, 1062, 1176
Eco24I GRGCYC 1 cut(s) 426
Eco47I GGWCC 4 cut(s) 562, 729, 1016, 1228
Eco47III AGCGCT 1 cut(s) 890
Eco57I CTGAAG 1 cut(s) 1026
Eco88I CYCGRG 2 cut(s) 527, 869
Eco91I GGTNACC 1 cut(s) 826
EcoO109I RGGNCCY 2 cut(s) 562, 1228
EcoO65I GGTNACC 1 cut(s) 826
EcoRII CCWGG 3 cut(s) 219, 660, 822
EcoT14I CCWWGG 4 cut(s) 267, 348, 1062, 1176
EcoT22I ATGCAT 1 cut(s) 994
EcoT38I GRGCYC 1 cut(s) 426
EgeI GGCGCC 2 cut(s) 140, 346
EheI GGCGCC 2 cut(s) 140, 346
ErhI CCWWGG 4 cut(s) 267, 348, 1062, 1176
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 7 cut(s) 206, 271, 433, 838, 992, 1180, 1213
FalI AAGNNNNNCTT 4 cut(s) 341, 373, 1205, 1237
FaqI GGGAC 3 cut(s) 362, 575, 889
FatI CATG 7 cut(s) 202, 267, 429, 834, 988, 1176, 1209
FauI CCCGC 1 cut(s) 308
FauNDI CATATG 1 cut(s) 994
Fnu4HI GCNGC 8 cut(s) 48, 81, 84, 157, 750, 757, 892, 1193
FokI GGATG 1 cut(s) 497
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 8 cut(s) 48, 81, 84, 157, 750, 757, 892, 1193
GlaI GCGC 4 cut(s) 140, 346, 890, 931
GluI GCNGC 8 cut(s) 48, 81, 84, 157, 750, 757, 892, 1193
GsaI CCCAGC 1 cut(s) 944
GsuI CTGGAG 2 cut(s) 515, 974
HaeII RGCGCY 3 cut(s) 142, 348, 892
HaeIII GGCC 4 cut(s) 875, 999, 1043, 1238
HapII CCGG 7 cut(s) 236, 380, 528, 924, 1014, 1019, 1275
HgaI GACGC 1 cut(s) 709
HhaI GCGC 4 cut(s) 141, 347, 891, 932
Hin1I GRCGYC 3 cut(s) 139, 345, 596
Hin1II CATG 7 cut(s) 206, 271, 433, 838, 992, 1180, 1213
Hin6I GCGC 4 cut(s) 139, 345, 889, 930
HinP1I GCGC 4 cut(s) 139, 345, 889, 930
HincII GTYRAC 1 cut(s) 1126
HindII GTYRAC 1 cut(s) 1126
HindIII AAGCTT 1 cut(s) 1055
HinfI GANTC 3 cut(s) 464, 1111, 1136
HpaII CCGG 7 cut(s) 236, 380, 528, 924, 1014, 1019, 1275
HphI GGTGA 5 cut(s) 46, 459, 820, 994, 1264
Hpy166II GTNNAC 4 cut(s) 364, 534, 619, 1126
Hpy188I TCNGA 5 cut(s) 421, 601, 779, 915, 1141
Hpy188III TCNNGA 5 cut(s) 70, 448, 1163, 1210, 1255
Hpy8I GTNNAC 4 cut(s) 364, 534, 619, 1126
Hpy99I CGWCG 2 cut(s) 598, 1169
HpyAV CCTTC 4 cut(s) 113, 742, 1126, 1207
HpyCH4IV ACGT 5 cut(s) 242, 596, 768, 1128, 1334
HpyCH4V TGCA 3 cut(s) 253, 720, 992
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 92, 651
HpyF3I CTNAG 2 cut(s) 340, 538
HpySE526I ACGT 5 cut(s) 242, 596, 768, 1128, 1334
Hsp92I GRCGYC 3 cut(s) 139, 345, 596
Hsp92II CATG 7 cut(s) 206, 271, 433, 838, 992, 1180, 1213
HspAI GCGC 4 cut(s) 139, 345, 889, 930
KasI GGCGCC 2 cut(s) 138, 344
KflI GGGWCCC 1 cut(s) 562
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 2 cut(s) 897, 1324
LmnI GCTCC 6 cut(s) 164, 229, 678, 708, 850, 949
Lsp1109I GCAGC 4 cut(s) 34, 143, 878, 1179
LweI GCATC 4 cut(s) 240, 298, 423, 1215
MabI ACCWGGT 1 cut(s) 822
MaeII ACGT 5 cut(s) 242, 596, 768, 1128, 1334
MaeIII GTNAC 3 cut(s) 826, 982, 1217
MalI GATC 2 cut(s) 899, 1326
MboI GATC 2 cut(s) 897, 1324
MboII GAAGA 5 cut(s) 85, 325, 346, 847, 1261
MhlI GDGCHC 5 cut(s) 267, 285, 426, 647, 847
MluCI AATT 3 cut(s) 40, 415, 577
Mly113I GGCGCC 2 cut(s) 139, 345
MlyI GAGTC 3 cut(s) 458, 1105, 1130
Mph1103I ATGCAT 1 cut(s) 994
MseI TTAA 2 cut(s) 650, 1083
MslI CAYNNNNRTG 3 cut(s) 630, 833, 993
MspA1I CMGCKG 2 cut(s) 83, 156
MspI CCGG 7 cut(s) 236, 380, 528, 924, 1014, 1019, 1275
MspR9I CCNGG 6 cut(s) 221, 380, 528, 529, 662, 824
MvaI CCWGG 3 cut(s) 221, 662, 824
MvnI CGCG 3 cut(s) 83, 756, 791
MwoI GCNNNNNNNGC 3 cut(s) 44, 92, 651
NarI GGCGCC 2 cut(s) 139, 345
NciI CCSGG 3 cut(s) 380, 528, 529
NcoI CCATGG 2 cut(s) 267, 1176
NdeI CATATG 1 cut(s) 994
NdeII GATC 2 cut(s) 897, 1324
NlaIII CATG 7 cut(s) 206, 271, 433, 838, 992, 1180, 1213
NlaIV GGNNCC 8 cut(s) 140, 225, 264, 346, 563, 564, 763, 1230
NmuCI GTSAC 2 cut(s) 826, 982
NsiI ATGCAT 1 cut(s) 994
NspI RCATGY 2 cut(s) 838, 992
OliI CACNNNNGTG 1 cut(s) 630
PaeR7I CTCGAG 1 cut(s) 869
PagI TCATGA 1 cut(s) 1209
PciI ACATGT 1 cut(s) 834
PcsI WCGNNNNNNNCGW 1 cut(s) 891
Pfl23II CGTACG 1 cut(s) 792
PflMI CCANNNNNTGG 1 cut(s) 1300
PinAI ACCGGT 1 cut(s) 923
PkrI GCNGC 8 cut(s) 49, 82, 85, 158, 751, 758, 893, 1194
PleI GAGTC 3 cut(s) 458, 1105, 1130
PluTI GGCGCC 2 cut(s) 142, 348
PpsI GAGTC 3 cut(s) 458, 1105, 1130
PpuMI RGGWCCY 2 cut(s) 562, 1228
PscI ACATGT 1 cut(s) 834
Psp5II RGGWCCY 2 cut(s) 562, 1228
Psp6I CCWGG 3 cut(s) 219, 660, 822
PspEI GGTNACC 1 cut(s) 826
PspFI CCCAGC 1 cut(s) 940
PspGI CCWGG 3 cut(s) 219, 660, 822
PspLI CGTACG 1 cut(s) 792
PspN4I GGNNCC 8 cut(s) 140, 225, 264, 346, 563, 564, 763, 1230
PspPI GGNCC 4 cut(s) 562, 729, 1016, 1228
PspPPI RGGWCCY 2 cut(s) 562, 1228
PspXI VCTCGAGB 1 cut(s) 869
RsaI GTAC 3 cut(s) 7, 620, 794
RsaNI GTAC 3 cut(s) 6, 619, 793
RseI CAYNNNNRTG 3 cut(s) 630, 833, 993
Rsr2I CGGWCCG 1 cut(s) 1016
RsrII CGGWCCG 1 cut(s) 1016
SacII CCGCGG 1 cut(s) 84
SaqAI TTAA 2 cut(s) 650, 1083
SatI GCNGC 8 cut(s) 48, 81, 84, 157, 750, 757, 892, 1193
Sau3AI GATC 2 cut(s) 897, 1324
Sau96I GGNCC 4 cut(s) 562, 729, 1016, 1228
SchI GAGTC 3 cut(s) 458, 1105, 1130
ScrFI CCNGG 6 cut(s) 221, 380, 528, 529, 662, 824
SduI GDGCHC 5 cut(s) 267, 285, 426, 647, 847
SexAI ACCWGGT 1 cut(s) 822
SfaNI GCATC 4 cut(s) 240, 298, 423, 1215
SfoI GGCGCC 2 cut(s) 140, 346
Sfr274I CTCGAG 1 cut(s) 869
Sfr303I CCGCGG 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 1274
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 4 cut(s) 562, 729, 1016, 1228
SlaI CTCGAG 1 cut(s) 869
SmaI CCCGGG 1 cut(s) 529
SmiMI CAYNNNNRTG 3 cut(s) 630, 833, 993
SmlI CTYRAG 2 cut(s) 640, 869
SmoI CTYRAG 2 cut(s) 640, 869
Sse9I AATT 3 cut(s) 40, 415, 577
SspDI GGCGCC 2 cut(s) 138, 344
StyD4I CCNGG 6 cut(s) 219, 378, 526, 527, 660, 822
StyI CCWWGG 4 cut(s) 267, 348, 1062, 1176
TaiI ACGT 5 cut(s) 245, 599, 771, 1131, 1337
TaqI TCGA 6 cut(s) 462, 481, 543, 870, 963, 1327
TasI AATT 3 cut(s) 40, 415, 577
TatI WGTACW 1 cut(s) 618
TauI GCSGC 4 cut(s) 83, 86, 752, 759
Tru1I TTAA 2 cut(s) 650, 1083
Tru9I TTAA 2 cut(s) 650, 1083
TscAI CASTG 1 cut(s) 722
TseFI GTSAC 2 cut(s) 826, 982
TseI GCWGC 4 cut(s) 47, 156, 891, 1192
Tsp45I GTSAC 2 cut(s) 826, 982
TspDTI ATGAA 4 cut(s) 29, 425, 516, 1226
TspGWI ACGGA 1 cut(s) 23
TspMI CCCGGG 1 cut(s) 527
TspRI CASTG 1 cut(s) 722
Van91I CCANNNNNTGG 1 cut(s) 1300
VpaK11BI GGWCC 4 cut(s) 562, 729, 1016, 1228
XceI RCATGY 2 cut(s) 838, 992
XcmI CCANNNNNNNNNTGG 1 cut(s) 436
XhoI CTCGAG 1 cut(s) 869
XmaI CCCGGG 1 cut(s) 527
ZraI GACGTC 1 cut(s) 597
Zsp2I ATGCAT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.