Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0003881

Physical Location & Seq

Genomic Coordinates Forward (+)

34147471 .. 34149418

Gene Structure 1948 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G025510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGGGTACGGAGATGAATATGCTTCCCAAATGTGAAGCCAATTACGCTGCTCTCACCCCAACGACTTTCTTGAAGAGAGCCGCGGCGTTTTATGCTAACCGCACCTCCCTCATCTACGAAGGCACTCGCTTCACATGGCGCCAAACCTACGACCGCTGCTCCCGTCTCGCATCCTCTCTTGTTTGTCTCAACATTGTCAAGCATGATGTTGTATCAGTTCTGGCTCCTAATGTTCCGGCAATGTATGAGATGCACTTTGCAGTGCCCATGGCTGGTGCGGTGCTCAACACCATCAACACCCGCCTTGATGCGAAGAACATAGCTTCCATTCTCAAACACTCAGGCGCCAAGGTCTTCTTTGTGGACTGCCAGTTCGTCCCGGTGGCGCGTGAGGCTCTCAAAATCCTTATGAATAATTCCGAGCCCTCCATGATGCCGTTGGTCATCGTGATTGATGACATCGACTCACCCACAGGTGTTCGATTGGGCGAACTGGAGTATGAACAACTCATCCGCAAGGGCAATCCCGGGTTTACCTCCGTCGAGCTTGACGACGAGTGGGACCCAATCGCTTTGAATTACACTTCTGGCACGACGTCTGAACCCAAAGGCGTTGTGTACAGCCACAGAGGTGCTTACTTGAGCACTTTAAGCCTAATCTTGGGTTGGGAAATGGGGAGCGAGCCTGTTTATCTATGGACGCTCCCTATGTTCCACTGCAACGGCTGGACCTTCACTTGGGGTGTGGCGGCACGCGGCGGAACCAACGTCTGCCTCCGAAACACCACCGCGTACGATATCTACCGCAACATCAAACGACACCAGGTCACCCACATGTGCTGTGCTCCCATCATCTTCAACATTCTCCTCGAGGCCAAACCCCACGAGCGCTGCGAGATCGTCTCCCCTATTCAGATACTTACCGGTGGAGCACCGCCCCCAGCTCCGCTGCTGGAGAAAATCGAGCCGTTGGGTTTCAAGGTGACACATGCATATGGCCTGACTGAAGCAACCGGACCGGCTTTGGTTTGCGAGTGGCAGGCCAAGTGGAACAAGCTTCCCAAGGAGGAACAATCAAATCTTAAAGCAAGGCAAGGAATAAGCATTTTGACTCTGGCTGATGTTGACGTGAAGGACCCGGAAACAATGGGGAGTGTTCCTCACGACGGCAAAACCATGGGGGAGATTGTGCTGCGTGGGAGTAGCATCATGAAGGGTTACTTCAAGGACCCAAAGGCCACCAACAAAGCGTTCAAGCACGGCTGGTTCTTCACCGGCGACGTCGGTGTTATCCACCCAGATGGTTATTTGGAGATCAAAGACAGATCGAAAGACGTGATCATATCAGGTGGGGAGAACATTAGTAGTGTAGAATTGGAGACCGTGCTTTACAGACACCCCAACATTCTGGAAGCTGCTGTGGTGGCAATGCCTCACCCACGGTGGGGAGAGACTCCTTGTGCTTTCGTGGCCCTTAGAAACAACGACTCTAACGTGAAAGTAAGCAAGTCTGATATCATCAGTTATTGTCGGAAGAACTTGCCGCATTTTATGGTTCCGAAAAAGGTTGAGTTTGTGCCTGAACTGCCCAAAAATTCAACCGGGAAGATTCTCAAGTTCGAGCTGAGGGAATGGGCAAAGGCCTTTGAACCGGTCGACGTATCGGAAAATCAGCAACAAGCCGTCATGGATTCTCACCCTTCTCGCTATAACGAGCAGACTCTGGCTTTCTCCCGGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0016143 GO:0016144 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018858 GO:0019605 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046459 GO:0047760 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

580

Amino Acids

64.71

Weight (kDa)

6.97

Isoelectric Point (pI)

37.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	25 - 408	2e-83	AMP-binding enzyme
AMP-binding_C	PF13193	458 - 536	2.8e-23	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65880 AT1G65890 AT1G66120
fragaria_vesca	FvH4_1g27440 FvH4_1g27450
malus_domestica	MD09G1280400.v1.1 MD17G1287600.v1.1
prunus_persica	Prupe.3G013700_v2.0.a1
pyrus_communis	pycom09g18850 pycom17g27060
rosa_chinensis	RchiOBHm_Chr3g0488561 RchiOBHm_Chr3g0488571
rosa_laevigata	RLG00000022942 RLG00000022943
rosa_multiflora	Rmu_sc0004402.1_g000006 Rmu_sc0004402.1_g000007 Rmu_sc0005363.1_g000024 Rmu_sc0005363.1_g000025
rosa_roxburghii	Rroxscaffold_6G00394130 Rroxscaffold_6G00394140
rosa_rugosa	Rorug03G0239300 Rorug03G0239400
rosa_samantha	Rh3AG288300 Rh3AG288400 Rh3BG325000 Rh3BG325100 Rh3CG322200 Rh3CG322300 Rh3DG321000 Rh3DG321100 Rh4AG012700 Rh7BG359000
rosa_wichuraiana	Rw3G025510 Rw3G025520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	599, 1284
AbsI	CCTCGAGG	1 cut(s)	869
AccB1I	GGYRCC	2 cut(s)	138, 344
AccI	GTMKAC	1 cut(s)	1656
AccII	CGCG	4 cut(s)	83, 388, 756, 791
AcsI	RAATTY	1 cut(s)	1594
AcuI	CTGAAG	1 cut(s)	1026
AcyI	GRCGYC	4 cut(s)	139, 345, 596, 1281
AfaI	GTAC	3 cut(s)	7, 620, 794
AfeI	AGCGCT	1 cut(s)	890
AfiI	CCNNNNNNNGG	6 cut(s)	272, 661, 738, 1166, 1444, 1445
AflIII	ACRYGT	1 cut(s)	834
AgeI	ACCGGT	2 cut(s)	923, 1651
AgsI	TTSAA	8 cut(s)	73, 577, 859, 979, 1225, 1255, 1599, 1649
AhdI	GACNNNNNGTC	1 cut(s)	824
AjiI	CACGTC	2 cut(s)	1129, 1336
AjnI	CCWGG	1 cut(s)	822
AjuI	GAANNNNNNNTTGG	2 cut(s)	1235, 1267
AleI	CACNNNNGTG	1 cut(s)	630
AloI	GAACNNNNNNTCC	6 cut(s)	308, 340, 356, 388, 495, 527
AluBI	AGCT	6 cut(s)	323, 547, 944, 1057, 1415, 1624
AluI	AGCT	6 cut(s)	323, 547, 944, 1057, 1415, 1624
Alw21I	GWGCWC	4 cut(s)	285, 647, 847, 934
Alw26I	GTCTC	5 cut(s)	170, 191, 907, 1373, 1445
AlwNI	CAGNNNCTG	1 cut(s)	1723
Ama87I	CYCGRG	2 cut(s)	527, 869
Aor51HI	AGCGCT	1 cut(s)	890
AoxI	GGCC	6 cut(s)	873, 997, 1041, 1236, 1470, 1641
ApeKI	GCWGC	6 cut(s)	47, 156, 891, 949, 1192, 1415
ApoI	RAATTY	1 cut(s)	1594
ArsI	GACNNNNNNTTYG	2 cut(s)	356, 388
AsiGI	ACCGGT	2 cut(s)	923, 1651
AspLEI	GCGC	4 cut(s)	141, 347, 388, 891
AspS9I	GGNCC	6 cut(s)	562, 729, 1016, 1135, 1228, 1471
AsuC2I	CCSGG	6 cut(s)	380, 528, 529, 1139, 1603, 1735
AsuHPI	GGTGA	7 cut(s)	46, 459, 820, 994, 1264, 1427, 1688
AvaI	CYCGRG	2 cut(s)	527, 869
AvaII	GGWCC	5 cut(s)	562, 729, 1016, 1135, 1228
BaeGI	GKGCMC	1 cut(s)	267
BanI	GGYRCC	2 cut(s)	138, 344
BanII	GRGCYC	1 cut(s)	426
BauI	CACGAG	1 cut(s)	884
BbsI	GAAGAC	1 cut(s)	346
Bbv12I	GWGCWC	4 cut(s)	285, 647, 847, 934
BbvCI	CCTCAGC	1 cut(s)	1625
BbvI	GCAGC	6 cut(s)	34, 143, 878, 936, 1179, 1402
BccI	CCATC	3 cut(s)	299, 857, 1295
BceAI	ACGGC	6 cut(s)	421, 739, 952, 1183, 1276, 1667
BciT130I	CCWGG	1 cut(s)	824
BclI	TGATCA	1 cut(s)	1338
BcnI	CCSGG	6 cut(s)	380, 528, 529, 1139, 1603, 1735
BcoDI	GTCTC	5 cut(s)	170, 191, 907, 1373, 1445
BfoI	RGCGCY	3 cut(s)	142, 348, 892
Bme1390I	CCNGG	7 cut(s)	380, 528, 529, 824, 1139, 1603, 1735
Bme18I	GGWCC	5 cut(s)	562, 729, 1016, 1135, 1228
BmeRI	GACNNNNNGTC	1 cut(s)	824
BmeT110I	CYCGRG	2 cut(s)	527, 869
BmgBI	CACGTC	2 cut(s)	1129, 1336
BmgT120I	GGNCC	6 cut(s)	562, 729, 1016, 1135, 1228, 1471
BmiI	GGNNCC	9 cut(s)	140, 225, 346, 563, 564, 763, 1137, 1230, 1557
BmrFI	CCNGG	7 cut(s)	380, 528, 529, 824, 1139, 1603, 1735
BmsI	GCATC	5 cut(s)	179, 240, 298, 423, 1215
BpiI	GAAGAC	1 cut(s)	346
BplI	GAGNNNNNCTC	4 cut(s)	887, 919, 1144, 1176
BpmI	CTGGAG	2 cut(s)	515, 974
Bpu10I	CCTNAGC	1 cut(s)	1625
BpuEI	CTTGAG	2 cut(s)	661, 1598
BpuMI	CCSGG	6 cut(s)	380, 528, 529, 1139, 1603, 1735
BsaHI	GRCGYC	4 cut(s)	139, 345, 596, 1281
BsaI	GGTCTC	1 cut(s)	1373
BsaJI	CCNNGG	7 cut(s)	81, 267, 348, 527, 1062, 1176, 1439
BsaWI	WCCGGW	3 cut(s)	923, 1013, 1651
BsaXI	ACNNNNNCTCC	4 cut(s)	89, 119, 143, 173
Bsc4I	CCNNNNNNNGG	6 cut(s)	272, 661, 738, 1166, 1444, 1445
Bse118I	RCCGGY	4 cut(s)	923, 1018, 1274, 1651
Bse1I	ACTGG	2 cut(s)	370, 498
Bse3DI	GCAATG	2 cut(s)	246, 1434
BseBI	CCWGG	1 cut(s)	824
BseDI	CCNNGG	7 cut(s)	81, 267, 348, 527, 1062, 1176, 1439
BseGI	GGATG	2 cut(s)	170, 510
BseLI	CCNNNNNNNGG	6 cut(s)	272, 661, 738, 1166, 1444, 1445
BseMI	GCAATG	2 cut(s)	246, 1434
BseMII	CTCAG	2 cut(s)	354, 1616
BseNI	ACTGG	2 cut(s)	370, 498
BseRI	GAGGAG	1 cut(s)	857
BseSI	GKGCMC	1 cut(s)	267
BseXI	GCAGC	6 cut(s)	34, 143, 878, 936, 1179, 1402
BseYI	CCCAGC	1 cut(s)	940
Bsh1236I	CGCG	4 cut(s)	83, 388, 756, 791
Bsh1285I	CGRYCG	2 cut(s)	154, 1656
BshFI	GGCC	6 cut(s)	875, 999, 1043, 1238, 1472, 1643
BshNI	GGYRCC	2 cut(s)	138, 344
BshTI	ACCGGT	2 cut(s)	923, 1651
BsiEI	CGRYCG	2 cut(s)	154, 1656
BsiHKAI	GWGCWC	4 cut(s)	285, 647, 847, 934
BsiHKCI	CYCGRG	2 cut(s)	527, 869
BsiWI	CGTACG	1 cut(s)	792
BslFI	GGGAC	2 cut(s)	362, 575
BslI	CCNNNNNNNGG	6 cut(s)	272, 661, 738, 1166, 1444, 1445
BsmAI	GTCTC	5 cut(s)	170, 191, 907, 1373, 1445
BsmBI	CGTCTC	2 cut(s)	170, 907
BsmFI	GGGAC	2 cut(s)	362, 575
BsnI	GGCC	6 cut(s)	875, 999, 1043, 1238, 1472, 1643
Bso31I	GGTCTC	1 cut(s)	1373
BsoBI	CYCGRG	2 cut(s)	527, 869
Bsp1286I	GDGCHC	6 cut(s)	267, 285, 426, 647, 847, 934
Bsp1407I	TGTACA	1 cut(s)	618
Bsp143I	GATC	4 cut(s)	897, 1314, 1325, 1338
Bsp19I	CCATGG	2 cut(s)	267, 1176
BspANI	GGCC	6 cut(s)	875, 999, 1043, 1238, 1472, 1643
BspCNI	CTCAG	2 cut(s)	353, 1617
BspFNI	CGCG	4 cut(s)	83, 388, 756, 791
BspHI	TCATGA	1 cut(s)	1209
BspLI	GGNNCC	9 cut(s)	140, 225, 346, 563, 564, 763, 1137, 1230, 1557
BspT107I	GGYRCC	2 cut(s)	138, 344
BspTNI	GGTCTC	1 cut(s)	1373
BsrDI	GCAATG	2 cut(s)	246, 1434
BsrFI	RCCGGY	4 cut(s)	923, 1018, 1274, 1651
BsrGI	TGTACA	1 cut(s)	618
BsrI	ACTGG	2 cut(s)	370, 498
BssAI	RCCGGY	4 cut(s)	923, 1018, 1274, 1651
BssECI	CCNNGG	7 cut(s)	81, 267, 348, 527, 1062, 1176, 1439
BssMI	GATC	4 cut(s)	897, 1314, 1325, 1338
BssNI	GRCGYC	4 cut(s)	139, 345, 596, 1281
BssSI	CACGAG	1 cut(s)	884
BssT1I	CCWWGG	4 cut(s)	267, 348, 1062, 1176
Bst2BI	CACGAG	1 cut(s)	884
Bst2UI	CCWGG	1 cut(s)	824
Bst4CI	ACNGT	2 cut(s)	1384, 1443
Bst6I	CTCTTC	1 cut(s)	68
BstACI	GRCGYC	4 cut(s)	139, 345, 596, 1281
BstAUI	TGTACA	1 cut(s)	618
BstC8I	GCNNGC	3 cut(s)	683, 754, 1041
BstDEI	CTNAG	3 cut(s)	340, 1475, 1625
BstDSI	CCRYGG	4 cut(s)	81, 267, 1176, 1439
BstEII	GGTNACC	1 cut(s)	826
BstF5I	GGATG	2 cut(s)	170, 510
BstFNI	CGCG	4 cut(s)	83, 388, 756, 791
BstH2I	RGCGCY	3 cut(s)	142, 348, 892
BstHHI	GCGC	4 cut(s)	141, 347, 388, 891
BstKTI	GATC	4 cut(s)	900, 1317, 1328, 1341
BstMAI	GTCTC	5 cut(s)	170, 191, 907, 1373, 1445
BstMBI	GATC	4 cut(s)	897, 1314, 1325, 1338
BstMCI	CGRYCG	2 cut(s)	154, 1656
BstMWI	GCNNNNNNNGC	7 cut(s)	44, 92, 392, 651, 1424, 1469, 1585
BstNI	CCWGG	1 cut(s)	824
BstNSI	RCATGY	2 cut(s)	838, 992
BstPI	GGTNACC	1 cut(s)	826
BstSCI	CCNGG	7 cut(s)	378, 526, 527, 822, 1137, 1601, 1733
BstSLI	GKGCMC	1 cut(s)	267
BstUI	CGCG	4 cut(s)	83, 388, 756, 791
BstV1I	GCAGC	6 cut(s)	34, 143, 878, 936, 1179, 1402
BstV2I	GAAGAC	1 cut(s)	346
BstXI	CCANNNNNNTGG	2 cut(s)	1301, 1408
BsuRI	GGCC	6 cut(s)	875, 999, 1043, 1238, 1472, 1643
BtgI	CCRYGG	4 cut(s)	81, 267, 1176, 1439
BtrI	CACGTC	2 cut(s)	1129, 1336
BtsCI	GGATG	2 cut(s)	170, 510
BtsI	GCAGTG	2 cut(s)	267, 715
BtsIMutI	CAGTG	2 cut(s)	267, 715
Cac8I	GCNNGC	3 cut(s)	683, 754, 1041
CaiI	CAGNNNCTG	1 cut(s)	1723
CciI	TCATGA	1 cut(s)	1209
CfoI	GCGC	4 cut(s)	141, 347, 388, 891
Cfr10I	RCCGGY	4 cut(s)	923, 1018, 1274, 1651
Cfr13I	GGNCC	6 cut(s)	562, 729, 1016, 1135, 1228, 1471
Cfr42I	CCGCGG	1 cut(s)	84
Cfr9I	CCCGGG	1 cut(s)	527
CpoI	CGGWCCG	1 cut(s)	1016
CseI	GACGC	1 cut(s)	709
CsiI	ACCWGGT	1 cut(s)	822
Csp6I	GTAC	3 cut(s)	6, 619, 793
CspAI	ACCGGT	2 cut(s)	923, 1651
CspI	CGGWCCG	1 cut(s)	1016
CviAII	CATG	9 cut(s)	135, 203, 268, 430, 835, 989, 1177, 1210, 1687
CviQI	GTAC	3 cut(s)	6, 619, 793
DdeI	CTNAG	3 cut(s)	340, 1475, 1625
DinI	GGCGCC	2 cut(s)	140, 346
DpnI	GATC	4 cut(s)	899, 1316, 1327, 1340
DpnII	GATC	4 cut(s)	897, 1314, 1325, 1338
DriI	GACNNNNNGTC	1 cut(s)	824
Eam1104I	CTCTTC	1 cut(s)	68
Eam1105I	GACNNNNNGTC	1 cut(s)	824
EarI	CTCTTC	1 cut(s)	68
EciI	GGCGGA	1 cut(s)	774
Eco130I	CCWWGG	4 cut(s)	267, 348, 1062, 1176
Eco147I	AGGCCT	1 cut(s)	1643
Eco24I	GRGCYC	1 cut(s)	426
Eco31I	GGTCTC	1 cut(s)	1373
Eco32I	GATATC	2 cut(s)	799, 1516
Eco47I	GGWCC	5 cut(s)	562, 729, 1016, 1135, 1228
Eco47III	AGCGCT	1 cut(s)	890
Eco57I	CTGAAG	1 cut(s)	1026
Eco88I	CYCGRG	2 cut(s)	527, 869
Eco91I	GGTNACC	1 cut(s)	826
EcoO109I	RGGNCCY	3 cut(s)	562, 1135, 1228
EcoO65I	GGTNACC	1 cut(s)	826
EcoRII	CCWGG	1 cut(s)	822
EcoRV	GATATC	2 cut(s)	799, 1516
EcoT14I	CCWWGG	4 cut(s)	267, 348, 1062, 1176
EcoT22I	ATGCAT	1 cut(s)	994
EcoT38I	GRGCYC	1 cut(s)	426
EgeI	GGCGCC	2 cut(s)	140, 346
EheI	GGCGCC	2 cut(s)	140, 346
ErhI	CCWWGG	4 cut(s)	267, 348, 1062, 1176
Esp3I	CGTCTC	2 cut(s)	170, 907
FaeI	CATG	9 cut(s)	138, 206, 271, 433, 838, 992, 1180, 1213, 1690
FalI	AAGNNNNNCTT	4 cut(s)	341, 373, 1205, 1237
FaqI	GGGAC	2 cut(s)	362, 575
FatI	CATG	9 cut(s)	134, 202, 267, 429, 834, 988, 1176, 1209, 1686
FauI	CCCGC	1 cut(s)	308
FauNDI	CATATG	1 cut(s)	994
FbaI	TGATCA	1 cut(s)	1338
FblI	GTMKAC	1 cut(s)	1656
FokI	GGATG	2 cut(s)	157, 497
FriOI	GRGCYC	1 cut(s)	426
GlaI	GCGC	4 cut(s)	140, 346, 387, 890
GsaI	CCCAGC	1 cut(s)	944
GsuI	CTGGAG	2 cut(s)	515, 974
HaeII	RGCGCY	3 cut(s)	142, 348, 892
HaeIII	GGCC	6 cut(s)	875, 999, 1043, 1238, 1472, 1643
HgaI	GACGC	1 cut(s)	709
HhaI	GCGC	4 cut(s)	141, 347, 388, 891
Hin1I	GRCGYC	4 cut(s)	139, 345, 596, 1281
Hin1II	CATG	9 cut(s)	138, 206, 271, 433, 838, 992, 1180, 1213, 1690
Hin6I	GCGC	4 cut(s)	139, 345, 386, 889
HinP1I	GCGC	4 cut(s)	139, 345, 386, 889
HincII	GTYRAC	2 cut(s)	1126, 1657
HindII	GTYRAC	2 cut(s)	1126, 1657
HindIII	AAGCTT	1 cut(s)	1055
HinfI	GANTC	7 cut(s)	464, 1111, 1453, 1487, 1609, 1691, 1720
HphI	GGTGA	7 cut(s)	46, 459, 820, 994, 1264, 1427, 1688
Hpy166II	GTNNAC	5 cut(s)	364, 534, 619, 1126, 1657
Hpy188I	TCNGA	8 cut(s)	421, 601, 779, 915, 1513, 1533, 1560, 1666
Hpy188III	TCNNGA	5 cut(s)	70, 448, 1163, 1210, 1409
Hpy8I	GTNNAC	5 cut(s)	364, 534, 619, 1126, 1657
Hpy99I	CGWCG	7 cut(s)	545, 557, 598, 1169, 1283, 1286, 1661
HpyAV	CCTTC	5 cut(s)	113, 742, 1126, 1207, 1710
HpyCH4III	ACNGT	2 cut(s)	1384, 1443
HpyCH4IV	ACGT	7 cut(s)	596, 768, 1128, 1281, 1335, 1494, 1659
HpyCH4V	TGCA	4 cut(s)	253, 260, 720, 992
HpyF10VI	GCNNNNNNNGC	7 cut(s)	44, 92, 392, 651, 1424, 1469, 1585
HpyF3I	CTNAG	3 cut(s)	340, 1475, 1625
HpySE526I	ACGT	7 cut(s)	596, 768, 1128, 1281, 1335, 1494, 1659
Hsp92I	GRCGYC	4 cut(s)	139, 345, 596, 1281
Hsp92II	CATG	9 cut(s)	138, 206, 271, 433, 838, 992, 1180, 1213, 1690
HspAI	GCGC	4 cut(s)	139, 345, 386, 889
KasI	GGCGCC	2 cut(s)	138, 344
KflI	GGGWCCC	1 cut(s)	562
Ksp22I	TGATCA	1 cut(s)	1338
KspI	CCGCGG	1 cut(s)	84
Kzo9I	GATC	4 cut(s)	897, 1314, 1325, 1338
LmnI	GCTCC	7 cut(s)	164, 229, 678, 708, 850, 929, 949
Lsp1109I	GCAGC	6 cut(s)	34, 143, 878, 936, 1179, 1402
LweI	GCATC	5 cut(s)	179, 240, 298, 423, 1215
MabI	ACCWGGT	1 cut(s)	822
MaeII	ACGT	7 cut(s)	596, 768, 1128, 1281, 1335, 1494, 1659
MaeIII	GTNAC	3 cut(s)	826, 982, 1217
MalI	GATC	4 cut(s)	899, 1316, 1327, 1340
MboI	GATC	4 cut(s)	897, 1314, 1325, 1338
MboII	GAAGA	7 cut(s)	85, 325, 346, 847, 1261, 1546, 1618
MhlI	GDGCHC	6 cut(s)	267, 285, 426, 647, 847, 934
MluCI	AATT	5 cut(s)	40, 415, 577, 1373, 1594
Mly113I	GGCGCC	2 cut(s)	139, 345
MlyI	GAGTC	5 cut(s)	458, 1105, 1447, 1481, 1714
MmeI	TCCRAC	1 cut(s)	1511
Mph1103I	ATGCAT	1 cut(s)	994
MseI	TTAA	3 cut(s)	650, 1083, 1741
MslI	CAYNNNNRTG	4 cut(s)	630, 833, 993, 1299
MspA1I	CMGCKG	3 cut(s)	83, 156, 949
MspR9I	CCNGG	7 cut(s)	380, 528, 529, 824, 1139, 1603, 1735
MvaI	CCWGG	1 cut(s)	824
MvnI	CGCG	4 cut(s)	83, 388, 756, 791
MwoI	GCNNNNNNNGC	7 cut(s)	44, 92, 392, 651, 1424, 1469, 1585
NarI	GGCGCC	2 cut(s)	139, 345
NciI	CCSGG	6 cut(s)	380, 528, 529, 1139, 1603, 1735
NcoI	CCATGG	2 cut(s)	267, 1176
NdeI	CATATG	1 cut(s)	994
NdeII	GATC	4 cut(s)	897, 1314, 1325, 1338
NlaIII	CATG	9 cut(s)	138, 206, 271, 433, 838, 992, 1180, 1213, 1690
NlaIV	GGNNCC	9 cut(s)	140, 225, 346, 563, 564, 763, 1137, 1230, 1557
NmuCI	GTSAC	2 cut(s)	826, 982
NsiI	ATGCAT	1 cut(s)	994
NspI	RCATGY	2 cut(s)	838, 992
OliI	CACNNNNGTG	1 cut(s)	630
PaeR7I	CTCGAG	1 cut(s)	869
PagI	TCATGA	1 cut(s)	1209
PceI	AGGCCT	1 cut(s)	1643
PciI	ACATGT	1 cut(s)	834
PcsI	WCGNNNNNNNCGW	3 cut(s)	549, 891, 1491
PfeI	GAWTC	2 cut(s)	1609, 1691
Pfl23II	CGTACG	1 cut(s)	792
PinAI	ACCGGT	2 cut(s)	923, 1651
PleI	GAGTC	5 cut(s)	458, 1105, 1447, 1481, 1714
PluTI	GGCGCC	2 cut(s)	142, 348
PpsI	GAGTC	5 cut(s)	458, 1105, 1447, 1481, 1714
PpuMI	RGGWCCY	3 cut(s)	562, 1135, 1228
PscI	ACATGT	1 cut(s)	834
Psp5II	RGGWCCY	3 cut(s)	562, 1135, 1228
Psp6I	CCWGG	1 cut(s)	822
PspEI	GGTNACC	1 cut(s)	826
PspFI	CCCAGC	1 cut(s)	940
PspGI	CCWGG	1 cut(s)	822
PspLI	CGTACG	1 cut(s)	792
PspN4I	GGNNCC	9 cut(s)	140, 225, 346, 563, 564, 763, 1137, 1230, 1557
PspPI	GGNCC	6 cut(s)	562, 729, 1016, 1135, 1228, 1471
PspPPI	RGGWCCY	3 cut(s)	562, 1135, 1228
PspXI	VCTCGAGB	1 cut(s)	869
PstNI	CAGNNNCTG	1 cut(s)	1723
RsaI	GTAC	3 cut(s)	7, 620, 794
RsaNI	GTAC	3 cut(s)	6, 619, 793
RseI	CAYNNNNRTG	4 cut(s)	630, 833, 993, 1299
Rsr2I	CGGWCCG	1 cut(s)	1016
RsrII	CGGWCCG	1 cut(s)	1016
SacII	CCGCGG	1 cut(s)	84
SalI	GTCGAC	1 cut(s)	1655
SaqAI	TTAA	3 cut(s)	650, 1083, 1741
Sau3AI	GATC	4 cut(s)	897, 1314, 1325, 1338
Sau96I	GGNCC	6 cut(s)	562, 729, 1016, 1135, 1228, 1471
SchI	GAGTC	5 cut(s)	458, 1105, 1447, 1481, 1714
ScrFI	CCNGG	7 cut(s)	380, 528, 529, 824, 1139, 1603, 1735
SduI	GDGCHC	6 cut(s)	267, 285, 426, 647, 847, 934
SexAI	ACCWGGT	1 cut(s)	822
SfaNI	GCATC	5 cut(s)	179, 240, 298, 423, 1215
SfoI	GGCGCC	2 cut(s)	140, 346
Sfr274I	CTCGAG	1 cut(s)	869
Sfr303I	CCGCGG	1 cut(s)	84
SgrAI	CRCCGGYG	1 cut(s)	1274
SgrBI	CCGCGG	1 cut(s)	84
SinI	GGWCC	5 cut(s)	562, 729, 1016, 1135, 1228
SlaI	CTCGAG	1 cut(s)	869
SmaI	CCCGGG	1 cut(s)	529
SmiMI	CAYNNNNRTG	4 cut(s)	630, 833, 993, 1299
SmlI	CTYRAG	3 cut(s)	640, 869, 1613
SmoI	CTYRAG	3 cut(s)	640, 869, 1613
Sse9I	AATT	5 cut(s)	40, 415, 577, 1373, 1594
SseBI	AGGCCT	1 cut(s)	1643
SspDI	GGCGCC	2 cut(s)	138, 344
StuI	AGGCCT	1 cut(s)	1643
StyD4I	CCNGG	7 cut(s)	378, 526, 527, 822, 1137, 1601, 1733
StyI	CCWWGG	4 cut(s)	267, 348, 1062, 1176
TaaI	ACNGT	2 cut(s)	1384, 1443
TaiI	ACGT	7 cut(s)	599, 771, 1131, 1284, 1338, 1497, 1662
TaqI	TCGA	8 cut(s)	462, 481, 543, 870, 963, 1328, 1620, 1656
TasI	AATT	5 cut(s)	40, 415, 577, 1373, 1594
TatI	WGTACW	1 cut(s)	618
TauI	GCSGC	5 cut(s)	83, 86, 752, 759, 1546
TfiI	GAWTC	2 cut(s)	1609, 1691
Tru1I	TTAA	3 cut(s)	650, 1083, 1741
Tru9I	TTAA	3 cut(s)	650, 1083, 1741
TscAI	CASTG	2 cut(s)	267, 722
TseFI	GTSAC	2 cut(s)	826, 982
TseI	GCWGC	6 cut(s)	47, 156, 891, 949, 1192, 1415
Tsp45I	GTSAC	2 cut(s)	826, 982
TspDTI	ATGAA	4 cut(s)	29, 425, 516, 1226
TspGWI	ACGGA	2 cut(s)	23, 529
TspMI	CCCGGG	1 cut(s)	527
TspRI	CASTG	2 cut(s)	267, 722
VpaK11BI	GGWCC	5 cut(s)	562, 729, 1016, 1135, 1228
XapI	RAATTY	1 cut(s)	1594
XceI	RCATGY	2 cut(s)	838, 992
XcmI	CCANNNNNNNNNTGG	1 cut(s)	436
XhoI	CTCGAG	1 cut(s)	869
XmaI	CCCGGG	1 cut(s)	527
XmiI	GTMKAC	1 cut(s)	1656
ZraI	GACGTC	2 cut(s)	597, 1282
Zsp2I	ATGCAT	1 cut(s)	994

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.