AT1G68070

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008516
Physical Location & Seq
Reverse (-)
25515232 .. 25517061
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68070.1

Sequence Viewer

Length: 1032 bp
ATGTCTTCACCTGAATCACCTTCCGGTTCCGACTCATCCACACCACTTCTCCGTTCACGGCAATCATCACCACGTCGTCAACCTGTAATCGCGGTTCTATTAAATCGAGCTTCAGGACGACGAGGAGCTTCGATGGTGGTTAGAGAAACAGCTGCACAAGAGCTTGAAGAGCGACGAGCTGATTGGGGTTACTCGAAACCTGTTGTTGCTTTGGATATGCTTTGGAACACTGCTTTTGTTCTTGTTGCGATTGTTATGCTTTTGGTTTTTAAAGAAGAGAAGCCAAATGTTCCTATTAGGATTTGGATCTGTGGTTATGCGATTCAGTGTCTTGTTCATGTTGTCCTTGTTTGGCTTGAGTTTAGAAAGAGGAATGCTAGAAGCAGACCTGGAGATTTAGAAGCTGCTCAAGCTACGAATCAGGATAGTGAAGATGAAGACAACGATGAGAGGTTTCTGAGCACTAAGACTTGTGAATCAATGAACACTATAATATCATTTGTCTGGTGGATTGTTGGATTCTACTGGCTTGTTTCTGGTGGTGATATCCTTCTGCAAAACGCGACGCATTTGTATTGGTTAACCTTTGTTTTCCTTGCATTTGATGTGTTCTTTGCCATCTTTTGCGTTGTGCTGGCATGTCTTATTGGAATCGCCCTCTGTTGTTGCCTACCTTGTATTATTGCTCTTCTTTACGCCGTCGCGGGGCAGGAGGGTGCATCAGAAGCAGATCTCAGCATCCTTCCCAAGTACAGGTTTCACACGATGAACAATGATGAAAAGCAAAGTGACGGTGGTGGGAAAATGATACCTGTGGATGCAGCGAGTGAGAATTTAGGAAACGAGCGTGTGCTTCTTCCCGAGGATGCTGACTGTTGTATATGTTTGAGTTCATATGAAGATGGAGCAGAGCTGGTCTCACTTCCTTGCAACCACCACTTTCATTCTACGTGCATTGTGAAATGGCTGAAAATGAATGCGACATGTCCACTATGCAAGTTCAATATCCTCAAAGGTAATGAACAAGAATGA

Protein Analysis

343

Amino Acids

38.3

Weight (kDa)

5.2

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 291 - 333 3.2e-15 Ring finger domain
zf-RING_11 PF17123 291 - 319 7.6e-10 RING-like zinc finger
zf-rbx1 PF12678 291 - 333 3e-09 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 292 - 332 9.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 292 - 332 5e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 92, 563, 704
AciI CCGC 2 cut(s) 92, 704
AclWI GGATC 1 cut(s) 314
AcsI RAATTY 1 cut(s) 832
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 1 cut(s) 752
AfiI CCNNNNNNNGG 2 cut(s) 705, 753
AflIII ACRYGT 1 cut(s) 983
AgsI TTSAA 2 cut(s) 167, 1003
AjiI CACGTC 1 cut(s) 74
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 8 cut(s) 110, 128, 152, 163, 179, 404, 413, 913
AluI AGCT 8 cut(s) 110, 128, 152, 163, 179, 404, 413, 913
Alw21I GWGCWC 1 cut(s) 464
Alw26I GTCTC 1 cut(s) 922
AlwI GGATC 1 cut(s) 314
Ama87I CYCGRG 1 cut(s) 860
ApeKI GCWGC 3 cut(s) 152, 404, 821
ApoI RAATTY 1 cut(s) 832
AsuHPI GGTGA 3 cut(s) 9, 60, 554
AvaI CYCGRG 1 cut(s) 860
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 464
BbvI GCAGC 3 cut(s) 139, 391, 833
BccI CCATC 3 cut(s) 127, 626, 896
BceAI ACGGC 2 cut(s) 74, 683
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BciT130I CCWGG 1 cut(s) 390
BcoDI GTCTC 1 cut(s) 922
BfaI CTAG 1 cut(s) 378
BglII AGATCT 1 cut(s) 730
BisI GCNGC 3 cut(s) 153, 405, 822
BlsI GCNGC 3 cut(s) 154, 406, 823
Bme1390I CCNGG 1 cut(s) 390
BmeT110I CYCGRG 1 cut(s) 860
BmgBI CACGTC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 390
BmsI GCATC 4 cut(s) 728, 747, 808, 856
BpiI GAAGAC 1 cut(s) 444
BplI GAGNNNNNCTC 2 cut(s) 902, 934
BpmI CTGGAG 1 cut(s) 411
BpuEI CTTGAG 2 cut(s) 377, 393
BsaAI YACGTR 1 cut(s) 951
BsaBI GATNNNNATC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 922
BsaJI CCNNGG 1 cut(s) 861
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 705, 753
Bse1I ACTGG 1 cut(s) 530
Bse8I GATNNNNATC 1 cut(s) 305
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 861
BseGI GGATG 4 cut(s) 35, 738, 823, 871
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 2 cut(s) 705, 753
BseMII CTCAG 2 cut(s) 449, 748
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 3 cut(s) 139, 391, 833
BsgI GTGCAG 1 cut(s) 138
Bsh1236I CGCG 3 cut(s) 92, 563, 704
BsiHKAI GWGCWC 1 cut(s) 464
BsiHKCI CYCGRG 1 cut(s) 860
BsiSI CCGG 1 cut(s) 24
BslI CCNNNNNNNGG 2 cut(s) 705, 753
BsmAI GTCTC 1 cut(s) 922
BsmI GAATGC 2 cut(s) 379, 982
Bso31I GGTCTC 1 cut(s) 922
BsoBI CYCGRG 1 cut(s) 860
Bsp1286I GDGCHC 1 cut(s) 464
Bsp143I GATC 2 cut(s) 306, 730
BspACI CCGC 2 cut(s) 92, 704
BspCNI CTCAG 2 cut(s) 450, 747
BspFNI CGCG 3 cut(s) 92, 563, 704
BspLI GGNNCC 1 cut(s) 28
BspPI GGATC 1 cut(s) 314
BspQI GCTCTTC 2 cut(s) 162, 693
BspTNI GGTCTC 1 cut(s) 922
BsrI ACTGG 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 861
BssMI GATC 2 cut(s) 306, 730
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 2 cut(s) 794, 875
Bst6I CTCTTC 3 cut(s) 162, 270, 693
BstBAI YACGTR 1 cut(s) 951
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 3 cut(s) 458, 465, 734
BstF5I GGATG 4 cut(s) 35, 738, 823, 871
BstFNI CGCG 3 cut(s) 92, 563, 704
BstKTI GATC 2 cut(s) 309, 733
BstMAI GTCTC 1 cut(s) 922
BstMBI GATC 2 cut(s) 306, 730
BstMWI GCNNNNNNNGC 3 cut(s) 169, 410, 725
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 2 cut(s) 642, 987
BstSCI CCNGG 1 cut(s) 388
BstUI CGCG 3 cut(s) 92, 563, 704
BstV1I GCAGC 3 cut(s) 139, 391, 833
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 2 cut(s) 306, 730
BstYI RGATCY 2 cut(s) 306, 730
BtrI CACGTC 1 cut(s) 74
BtsCI GGATG 4 cut(s) 35, 738, 823, 871
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 228, 332
Cac8I GCNNGC 1 cut(s) 636
CseI GACGC 1 cut(s) 574
Csp6I GTAC 1 cut(s) 751
CviAII CATG 3 cut(s) 338, 639, 984
CviQI GTAC 1 cut(s) 751
DdeI CTNAG 3 cut(s) 458, 465, 734
DpnI GATC 2 cut(s) 308, 732
DpnII GATC 2 cut(s) 306, 730
DraI TTTAAA 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 162, 270, 693
EarI CTCTTC 3 cut(s) 162, 270, 693
Eco31I GGTCTC 1 cut(s) 922
Eco32I GATATC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 860
EcoRII CCWGG 1 cut(s) 388
EcoRV GATATC 1 cut(s) 547
FaeI CATG 3 cut(s) 341, 642, 987
FatI CATG 3 cut(s) 337, 638, 983
FauI CCCGC 1 cut(s) 697
FauNDI CATATG 1 cut(s) 895
Fnu4HI GCNGC 3 cut(s) 153, 405, 822
FokI GGATG 4 cut(s) 22, 725, 830, 878
Fsp4HI GCNGC 3 cut(s) 153, 405, 822
FspBI CTAG 1 cut(s) 378
GluI GCNGC 3 cut(s) 153, 405, 822
GsuI CTGGAG 1 cut(s) 411
HapII CCGG 1 cut(s) 24
HgaI GACGC 1 cut(s) 574
Hin1II CATG 3 cut(s) 341, 642, 987
HincII GTYRAC 2 cut(s) 80, 582
HindII GTYRAC 2 cut(s) 80, 582
HinfI GANTC 7 cut(s) 14, 32, 322, 418, 476, 519, 651
HpaI GTTAAC 1 cut(s) 582
HpaII CCGG 1 cut(s) 24
HphI GGTGA 3 cut(s) 9, 60, 554
Hpy166II GTNNAC 4 cut(s) 56, 80, 582, 989
Hpy188I TCNGA 3 cut(s) 31, 459, 724
Hpy188III TCNNGA 3 cut(s) 114, 422, 860
Hpy8I GTNNAC 4 cut(s) 56, 80, 582, 989
Hpy99I CGWCG 5 cut(s) 78, 123, 177, 568, 704
HpyAV CCTTC 3 cut(s) 30, 560, 752
HpyCH4III ACNGT 2 cut(s) 794, 875
HpyCH4IV ACGT 2 cut(s) 73, 950
HpyCH4V TGCA 8 cut(s) 155, 556, 599, 719, 821, 930, 954, 996
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 410, 725
HpyF3I CTNAG 3 cut(s) 458, 465, 734
HpySE526I ACGT 2 cut(s) 73, 950
Hsp92II CATG 3 cut(s) 341, 642, 987
KspAI GTTAAC 1 cut(s) 582
Kzo9I GATC 2 cut(s) 306, 730
LguI GCTCTTC 2 cut(s) 162, 693
LmnI GCTCC 2 cut(s) 125, 905
Lsp1109I GCAGC 3 cut(s) 139, 391, 833
LweI GCATC 4 cut(s) 728, 747, 808, 856
MaeI CTAG 1 cut(s) 378
MaeII ACGT 2 cut(s) 73, 950
MaeIII GTNAC 2 cut(s) 188, 788
MalI GATC 2 cut(s) 308, 732
MboI GATC 2 cut(s) 306, 730
MboII GAAGA 7 cut(s) 179, 287, 443, 449, 680, 848, 911
MflI RGATCY 2 cut(s) 306, 730
MhlI GDGCHC 1 cut(s) 464
MluCI AATT 1 cut(s) 832
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 54, 496
MnlI CCTC 7 cut(s) 116, 363, 444, 668, 706, 856, 1019
MseI TTAA 3 cut(s) 101, 270, 581
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 390
Mva1269I GAATGC 2 cut(s) 379, 982
MvaI CCWGG 1 cut(s) 390
MvnI CGCG 3 cut(s) 92, 563, 704
MwoI GCNNNNNNNGC 3 cut(s) 169, 410, 725
NdeI CATATG 1 cut(s) 895
NdeII GATC 2 cut(s) 306, 730
NlaIII CATG 3 cut(s) 341, 642, 987
NlaIV GGNNCC 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 788
NspI RCATGY 2 cut(s) 642, 987
PciI ACATGT 1 cut(s) 983
PciSI GCTCTTC 2 cut(s) 162, 693
PctI GAATGC 2 cut(s) 379, 982
PfeI GAWTC 6 cut(s) 14, 322, 418, 476, 519, 651
PkrI GCNGC 3 cut(s) 154, 406, 823
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
Ppu21I YACGTR 1 cut(s) 951
PscI ACATGT 1 cut(s) 983
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 28
PsrI GAACNNNNNNTAC 2 cut(s) 78, 110
PsuI RGATCY 2 cut(s) 306, 730
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 1 cut(s) 752
RsaNI GTAC 1 cut(s) 751
SapI GCTCTTC 2 cut(s) 162, 693
SaqAI TTAA 3 cut(s) 101, 270, 581
SatI GCNGC 3 cut(s) 153, 405, 822
Sau3AI GATC 2 cut(s) 306, 730
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 1 cut(s) 464
SfaNI GCATC 4 cut(s) 728, 747, 808, 856
SmlI CTYRAG 2 cut(s) 356, 408
SmoI CTYRAG 2 cut(s) 356, 408
Sse9I AATT 1 cut(s) 832
SsiI CCGC 2 cut(s) 92, 704
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 794, 875
TaiI ACGT 2 cut(s) 76, 953
TaqI TCGA 3 cut(s) 106, 131, 194
TasI AATT 1 cut(s) 832
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 6 cut(s) 14, 322, 418, 476, 519, 651
Tru1I TTAA 3 cut(s) 101, 270, 581
Tru9I TTAA 3 cut(s) 101, 270, 581
TscAI CASTG 2 cut(s) 235, 332
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 3 cut(s) 152, 404, 821
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 9 cut(s) 326, 450, 497, 782, 792, 882, 912, 932, 989
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 2 cut(s) 235, 332
XapI RAATTY 1 cut(s) 832
XceI RCATGY 2 cut(s) 642, 987
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.