Rh4BG409700

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008516
Physical Location & Seq
Forward (+)
55932412 .. 55935117
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG409700.1

Sequence Viewer

Length: 1107 bp
ATGTCATCGGGCTCTTCAGCTACGGCGGCGACGGCGACGTCCACCGAGTCACGCGCGCCGCTTCTGCTTCCCCGGCAGGGGAGCGACGGCCGTGACTTGTCGGACCTCGGCTCCCCGACCCGCCAGGCCACGCTGGCCATGCTGCTGGGCCGGGCCACGGGCCGGCGAGGGCCGTCCATGCTGGTGAGGGAGACGGCGGCGCGCGAGCTGGACGAGCGGCGCGCCGACTGGGGGTACTCGAAGCCGGTGGTGGCTCTGGACATGATGTGGAACACGGCGTTCGTGGTGGTCTCGGTGGTGATGCTGGTTCTGACCCGAGACGAGAACCCGAACACGCCGATCCGGCTCTGGATCTGCGGCTACGCGCTGCAGTGCTTTGTCCACGTGGCGCTGGTGTGGATTGAGTACCGGAGGAGGAATACGAGGAGAGGGAGGAGAGGGCCGGAGGTGCTGCAGCAGAGCTCCGAAGCTGCCGAGGCTAATGATACTGATGATGATGAAACAGCTACGGATTTGGGGGTTCAGAGTCGTTCCAGTTGCTCTAAACGATGTGAATCGGTCAATACAATGGCATCCTTTCTCTGGTGGATAGTTGGCTTTTATTGGGTAGTCTCCGGTGGTGATTTGCTCCTGCAAAATGCTCCGCGTTTGTACTGGTTGTCTGTGGTTTTCCTGGCATTTGACGTCTTCTTTGCCATCTTTTGTGTTGTTCTGGCATGTTTGATCGGAATTGCTCTTTGTTGCTGTTTGCCATGCATCATTGCAATTCTATATGCTGTGGCAGGACAGGAAGGTGCATCCGAATCAGATCTCAGTATTCTTCCAAAATACAGATTTAGAATATCAGGCGATGAAAAATCTAGTGTAGGAGCAGGGAAAATGATTCCAATGGAATCAACCAGTGGGTACCTGGCAGTCGAGCGTATTCTTTTACCAGAGGATGCGGAATGTAGTATATGTCTCAGCCAATATGAGGATGGGGCAGATCTTCACACTCTTCCCTGTAACCATCACTTCCATGCTAATTGCATTGTGAAATGGCTTAAAATGAATGCAACATGTCCTCTCTGCAAATACAATATTCTCAAGGGAAGCGAACAGGTTTAA

Protein Analysis

368

Amino Acids

40.56

Weight (kDa)

5.82

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 315 - 358 1.9e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 316 - 358 1e-15 Ring finger domain
zf-RING_11 PF17123 316 - 344 1.1e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 316 - 357 7.7e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 317 - 357 1.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 317 - 355 5.4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 37
AatII GACGTC 2 cut(s) 41, 687
Acc65I GGTACC 1 cut(s) 906
AccB1I GGYRCC 1 cut(s) 906
AccBSI CCGCTC 1 cut(s) 217
AccII CGCG 7 cut(s) 54, 56, 202, 204, 222, 365, 646
AciI CCGC 8 cut(s) 26, 59, 121, 197, 217, 357, 644, 944
AclWI GGATC 2 cut(s) 334, 359
AcoI YGGCCR 2 cut(s) 88, 135
AcvI CACGTG 1 cut(s) 385
AcyI GRCGYC 2 cut(s) 38, 684
AfaI GTAC 4 cut(s) 236, 407, 653, 908
AfiI CCNNNNNNNGG 7 cut(s) 77, 78, 157, 162, 231, 582, 973
AflIII ACRYGT 1 cut(s) 1058
AjnI CCWGG 3 cut(s) 123, 672, 909
AluBI AGCT 5 cut(s) 20, 208, 462, 470, 506
AluI AGCT 5 cut(s) 20, 208, 462, 470, 506
Alw21I GWGCWC 1 cut(s) 464
Alw26I GTCTC 5 cut(s) 185, 295, 312, 616, 965
AlwI GGATC 2 cut(s) 334, 359
Ama87I CYCGRG 1 cut(s) 315
AoxI GGCC 8 cut(s) 88, 126, 135, 148, 153, 160, 170, 440
ApeKI GCWGC 5 cut(s) 142, 367, 451, 454, 470
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AscI GGCGCGCC 1 cut(s) 220
Asp718I GGTACC 1 cut(s) 906
AspLEI GCGC 8 cut(s) 56, 58, 202, 204, 222, 224, 367, 391
AspS9I GGNCC 6 cut(s) 103, 148, 153, 160, 170, 440
AsuC2I CCSGG 2 cut(s) 73, 152
AsuHPI GGTGA 3 cut(s) 196, 310, 632
AvaI CYCGRG 1 cut(s) 315
AvaII GGWCC 1 cut(s) 103
BalI TGGCCA 1 cut(s) 137
BanI GGYRCC 1 cut(s) 906
BanII GRGCYC 2 cut(s) 14, 464
BbrPI CACGTG 1 cut(s) 385
BbsI GAAGAC 1 cut(s) 679
Bbv12I GWGCWC 1 cut(s) 464
BbvI GCAGC 5 cut(s) 129, 354, 438, 457, 466
BccI CCATC 3 cut(s) 704, 971, 1017
BceAI ACGGC 7 cut(s) 39, 48, 75, 103, 157, 210, 291
BciT130I CCWGG 3 cut(s) 125, 674, 911
BcnI CCSGG 2 cut(s) 73, 152
BcoDI GTCTC 5 cut(s) 185, 295, 312, 616, 965
BfaI CTAG 1 cut(s) 861
BfmI CTRYAG 2 cut(s) 368, 452
BfoI RGCGCY 1 cut(s) 392
BglI GCCNNNNNGGC 2 cut(s) 134, 343
BglII AGATCT 2 cut(s) 808, 985
Bme1390I CCNGG 5 cut(s) 73, 125, 152, 674, 911
Bme18I GGWCC 1 cut(s) 103
BmeT110I CYCGRG 1 cut(s) 315
BmgT120I GGNCC 6 cut(s) 103, 148, 153, 160, 170, 440
BmiI GGNNCC 2 cut(s) 112, 908
BmrFI CCNGG 5 cut(s) 73, 125, 152, 674, 911
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 5 cut(s) 291, 581, 765, 806, 931
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 679
BpuEI CTTGAG 1 cut(s) 1070
BpuMI CCSGG 2 cut(s) 73, 152
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 553
BsaHI GRCGYC 2 cut(s) 38, 684
BsaI GGTCTC 1 cut(s) 295
BsaJI CCNNGG 4 cut(s) 71, 106, 156, 474
BsaWI WCCGGW 2 cut(s) 408, 614
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 7 cut(s) 77, 78, 157, 162, 231, 582, 973
Bse118I RCCGGY 2 cut(s) 162, 244
Bse1I ACTGG 4 cut(s) 233, 534, 659, 900
Bse3DI GCAATG 1 cut(s) 759
Bse8I GATNNNNATC 1 cut(s) 553
BseBI CCWGG 3 cut(s) 125, 674, 911
BseDI CCNNGG 4 cut(s) 71, 106, 156, 474
BseGI GGATG 4 cut(s) 572, 797, 946, 982
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 7 cut(s) 77, 78, 157, 162, 231, 582, 973
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 2 cut(s) 826, 976
BseNI ACTGG 4 cut(s) 233, 534, 659, 900
BsePI GCGCGC 3 cut(s) 54, 200, 220
BseRI GAGGAG 3 cut(s) 427, 439, 448
BseX3I CGGCCG 1 cut(s) 88
BseXI GCAGC 5 cut(s) 129, 354, 438, 457, 466
BseYI CCCAGC 1 cut(s) 145
Bsh1236I CGCG 7 cut(s) 54, 56, 202, 204, 222, 365, 646
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 8 cut(s) 90, 128, 137, 150, 155, 162, 172, 442
BshNI GGYRCC 1 cut(s) 906
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 464
BsiHKCI CYCGRG 1 cut(s) 315
BsiSI CCGG 8 cut(s) 73, 151, 163, 245, 343, 409, 443, 615
BslI CCNNNNNNNGG 7 cut(s) 77, 78, 157, 162, 231, 582, 973
BsmAI GTCTC 5 cut(s) 185, 295, 312, 616, 965
BsmBI CGTCTC 2 cut(s) 185, 312
BsmI GAATGC 1 cut(s) 1057
BsnI GGCC 8 cut(s) 90, 128, 137, 150, 155, 162, 172, 442
Bso31I GGTCTC 1 cut(s) 295
BsoBI CYCGRG 1 cut(s) 315
Bsp1286I GDGCHC 2 cut(s) 14, 464
Bsp143I GATC 5 cut(s) 339, 351, 723, 808, 985
BspACI CCGC 8 cut(s) 26, 59, 121, 197, 217, 357, 644, 944
BspANI GGCC 8 cut(s) 90, 128, 137, 150, 155, 162, 172, 442
BspCNI CTCAG 2 cut(s) 825, 975
BspFNI CGCG 7 cut(s) 54, 56, 202, 204, 222, 365, 646
BspLI GGNNCC 2 cut(s) 112, 908
BspMAI CTGCAG 2 cut(s) 372, 456
BspPI GGATC 2 cut(s) 334, 359
BspQI GCTCTTC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 906
BspTNI GGTCTC 1 cut(s) 295
BsrBI CCGCTC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 2 cut(s) 162, 244
BsrI ACTGG 4 cut(s) 233, 534, 659, 900
BssAI RCCGGY 2 cut(s) 162, 244
BssECI CCNNGG 4 cut(s) 71, 106, 156, 474
BssHII GCGCGC 3 cut(s) 54, 200, 220
BssMI GATC 5 cut(s) 339, 351, 723, 808, 985
BssNI GRCGYC 2 cut(s) 38, 684
Bst2UI CCWGG 3 cut(s) 125, 674, 911
Bst6I CTCTTC 2 cut(s) 19, 1002
BstACI GRCGYC 2 cut(s) 38, 684
BstBAI YACGTR 1 cut(s) 385
BstC8I GCNNGC 6 cut(s) 56, 135, 164, 202, 206, 222
BstDEI CTNAG 2 cut(s) 812, 962
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 4 cut(s) 572, 797, 946, 982
BstFNI CGCG 7 cut(s) 54, 56, 202, 204, 222, 365, 646
BstH2I RGCGCY 1 cut(s) 392
BstHHI GCGC 8 cut(s) 56, 58, 202, 204, 222, 224, 367, 391
BstKTI GATC 5 cut(s) 342, 354, 726, 811, 988
BstMAI GTCTC 5 cut(s) 185, 295, 312, 616, 965
BstMBI GATC 5 cut(s) 339, 351, 723, 808, 985
BstMCI CGRYCG 1 cut(s) 91
BstNI CCWGG 3 cut(s) 125, 674, 911
BstNSI RCATGY 2 cut(s) 720, 1062
BstSCI CCNGG 5 cut(s) 71, 123, 150, 672, 909
BstSFI CTRYAG 2 cut(s) 368, 452
BstUI CGCG 7 cut(s) 54, 56, 202, 204, 222, 365, 646
BstV1I GCAGC 5 cut(s) 129, 354, 438, 457, 466
BstV2I GAAGAC 1 cut(s) 679
BstX2I RGATCY 3 cut(s) 351, 808, 985
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 3 cut(s) 351, 808, 985
BstZI CGGCCG 1 cut(s) 88
BsuRI GGCC 8 cut(s) 90, 128, 137, 150, 155, 162, 172, 442
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 864
BtsCI GGATG 4 cut(s) 572, 797, 946, 982
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 2 cut(s) 377, 907
Cac8I GCNNGC 6 cut(s) 56, 135, 164, 202, 206, 222
CfoI GCGC 8 cut(s) 56, 58, 202, 204, 222, 224, 367, 391
Cfr10I RCCGGY 2 cut(s) 162, 244
Cfr13I GGNCC 6 cut(s) 103, 148, 153, 160, 170, 440
Csp6I GTAC 4 cut(s) 235, 406, 652, 907
CviAII CATG 7 cut(s) 139, 178, 262, 717, 753, 1019, 1059
CviQI GTAC 4 cut(s) 235, 406, 652, 907
DdeI CTNAG 2 cut(s) 812, 962
DpnI GATC 5 cut(s) 341, 353, 725, 810, 987
DpnII GATC 5 cut(s) 339, 351, 723, 808, 985
DrdI GACNNNNNNGTC 1 cut(s) 37
DseDI GACNNNNNNGTC 1 cut(s) 37
EaeI YGGCCR 2 cut(s) 88, 135
EagI CGGCCG 1 cut(s) 88
Eam1104I CTCTTC 2 cut(s) 19, 1002
EarI CTCTTC 2 cut(s) 19, 1002
Ecl136II GAGCTC 1 cut(s) 462
EclXI CGGCCG 1 cut(s) 88
Eco24I GRGCYC 2 cut(s) 14, 464
Eco31I GGTCTC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 103
Eco52I CGGCCG 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 462
Eco72I CACGTG 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 462
EcoRII CCWGG 3 cut(s) 123, 672, 909
EcoT22I ATGCAT 1 cut(s) 758
EcoT38I GRGCYC 2 cut(s) 14, 464
Esp3I CGTCTC 2 cut(s) 185, 312
FaeI CATG 7 cut(s) 142, 181, 265, 720, 756, 1022, 1062
FatI CATG 7 cut(s) 138, 177, 261, 716, 752, 1018, 1058
FauI CCCGC 1 cut(s) 128
FokI GGATG 4 cut(s) 559, 784, 953, 989
FriOI GRGCYC 2 cut(s) 14, 464
FspBI CTAG 1 cut(s) 861
GlaI GCGC 8 cut(s) 55, 57, 201, 203, 221, 223, 366, 390
GsaI CCCAGC 1 cut(s) 149
HaeII RGCGCY 1 cut(s) 392
HaeIII GGCC 8 cut(s) 90, 128, 137, 150, 155, 162, 172, 442
HapII CCGG 8 cut(s) 73, 151, 163, 245, 343, 409, 443, 615
HhaI GCGC 8 cut(s) 56, 58, 202, 204, 222, 224, 367, 391
Hin1I GRCGYC 2 cut(s) 38, 684
Hin1II CATG 7 cut(s) 142, 181, 265, 720, 756, 1022, 1062
Hin6I GCGC 8 cut(s) 54, 56, 200, 202, 220, 222, 365, 389
HinP1I GCGC 8 cut(s) 54, 56, 200, 202, 220, 222, 365, 389
HinfI GANTC 6 cut(s) 47, 526, 554, 803, 883, 893
HpaII CCGG 8 cut(s) 73, 151, 163, 245, 343, 409, 443, 615
HphI GGTGA 3 cut(s) 196, 310, 632
Hpy166II GTNNAC 2 cut(s) 42, 382
Hpy188I TCNGA 7 cut(s) 103, 312, 466, 525, 728, 802, 808
Hpy188III TCNNGA 2 cut(s) 257, 349
Hpy8I GTNNAC 2 cut(s) 42, 382
Hpy99I CGWCG 3 cut(s) 34, 40, 89
HpyAV CCTTC 1 cut(s) 785
HpyCH4IV ACGT 3 cut(s) 38, 384, 684
HpyCH4V TGCA 9 cut(s) 370, 454, 634, 756, 764, 797, 1029, 1055, 1071
HpyF3I CTNAG 2 cut(s) 812, 962
HpySE526I ACGT 3 cut(s) 38, 384, 684
Hsp92I GRCGYC 2 cut(s) 38, 684
Hsp92II CATG 7 cut(s) 142, 181, 265, 720, 756, 1022, 1062
HspAI GCGC 8 cut(s) 54, 56, 200, 202, 220, 222, 365, 389
KpnI GGTACC 1 cut(s) 910
KroI GCCGGC 1 cut(s) 162
KroNI GCCGGC 1 cut(s) 164
Kzo9I GATC 5 cut(s) 339, 351, 723, 808, 985
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 6 cut(s) 81, 116, 467, 633, 646, 869
Lsp1109I GCAGC 5 cut(s) 129, 354, 438, 457, 466
LweI GCATC 5 cut(s) 291, 581, 765, 806, 931
MaeI CTAG 1 cut(s) 861
MaeII ACGT 3 cut(s) 38, 384, 684
MaeIII GTNAC 3 cut(s) 48, 92, 1004
MalI GATC 5 cut(s) 341, 353, 725, 810, 987
MbiI CCGCTC 1 cut(s) 217
MboI GATC 5 cut(s) 339, 351, 723, 808, 985
MboII GAAGA 5 cut(s) 6, 679, 812, 980, 989
MflI RGATCY 3 cut(s) 351, 808, 985
MhlI GDGCHC 2 cut(s) 14, 464
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 3 cut(s) 729, 765, 1024
MluNI TGGCCA 1 cut(s) 137
MlyI GAGTC 2 cut(s) 56, 535
MmeI TCCRAC 1 cut(s) 81
Mox20I TGGCCA 1 cut(s) 137
Mph1103I ATGCAT 1 cut(s) 758
MroNI GCCGGC 1 cut(s) 162
MscI TGGCCA 1 cut(s) 137
MseI TTAA 2 cut(s) 1044, 1105
MslI CAYNNNNRTG 2 cut(s) 182, 1017
Msp20I TGGCCA 1 cut(s) 137
MspI CCGG 8 cut(s) 73, 151, 163, 245, 343, 409, 443, 615
MspR9I CCNGG 5 cut(s) 73, 125, 152, 674, 911
Mva1269I GAATGC 1 cut(s) 1057
MvaI CCWGG 3 cut(s) 125, 674, 911
MvnI CGCG 7 cut(s) 54, 56, 202, 204, 222, 365, 646
NaeI GCCGGC 1 cut(s) 164
NciI CCSGG 2 cut(s) 73, 152
NdeII GATC 5 cut(s) 339, 351, 723, 808, 985
NgoMIV GCCGGC 1 cut(s) 162
NlaIII CATG 7 cut(s) 142, 181, 265, 720, 756, 1022, 1062
NlaIV GGNNCC 2 cut(s) 112, 908
NmeAIII GCCGAG 2 cut(s) 87, 499
NmuCI GTSAC 2 cut(s) 48, 92
NsiI ATGCAT 1 cut(s) 758
NspI RCATGY 2 cut(s) 720, 1062
PalAI GGCGCGCC 1 cut(s) 220
PauI GCGCGC 3 cut(s) 54, 200, 220
PciI ACATGT 1 cut(s) 1058
PciSI GCTCTTC 1 cut(s) 19
PctI GAATGC 1 cut(s) 1057
PdiI GCCGGC 1 cut(s) 164
PfeI GAWTC 4 cut(s) 554, 803, 883, 893
PleI GAGTC 2 cut(s) 55, 534
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
PpsI GAGTC 2 cut(s) 55, 534
Ppu21I YACGTR 1 cut(s) 385
PscI ACATGT 1 cut(s) 1058
Psp124BI GAGCTC 1 cut(s) 464
Psp6I CCWGG 3 cut(s) 123, 672, 909
PspCI CACGTG 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 3 cut(s) 123, 672, 909
PspN4I GGNNCC 2 cut(s) 112, 908
PspPI GGNCC 6 cut(s) 103, 148, 153, 160, 170, 440
PstI CTGCAG 2 cut(s) 372, 456
PsuI RGATCY 3 cut(s) 351, 808, 985
PteI GCGCGC 3 cut(s) 54, 200, 220
RsaI GTAC 4 cut(s) 236, 407, 653, 908
RsaNI GTAC 4 cut(s) 235, 406, 652, 907
RseI CAYNNNNRTG 2 cut(s) 182, 1017
SacI GAGCTC 1 cut(s) 464
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 2 cut(s) 1044, 1105
Sau3AI GATC 5 cut(s) 339, 351, 723, 808, 985
Sau96I GGNCC 6 cut(s) 103, 148, 153, 160, 170, 440
SchI GAGTC 2 cut(s) 56, 535
ScrFI CCNGG 5 cut(s) 73, 125, 152, 674, 911
SduI GDGCHC 2 cut(s) 14, 464
SfaNI GCATC 5 cut(s) 291, 581, 765, 806, 931
SfcI CTRYAG 2 cut(s) 368, 452
SfiI GGCCNNNNNGGCC 1 cut(s) 134
SgsI GGCGCGCC 1 cut(s) 220
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 2 cut(s) 182, 1017
SmlI CTYRAG 1 cut(s) 1085
SmoI CTYRAG 1 cut(s) 1085
Sse9I AATT 3 cut(s) 729, 765, 1024
SsiI CCGC 8 cut(s) 26, 59, 121, 197, 217, 357, 644, 944
SspI AATATT 1 cut(s) 1081
SspMI CTAG 1 cut(s) 861
SstI GAGCTC 1 cut(s) 464
StyD4I CCNGG 5 cut(s) 71, 123, 150, 672, 909
TaiI ACGT 3 cut(s) 41, 387, 687
TaqI TCGA 2 cut(s) 239, 918
TaqII GACCGA 1 cut(s) 547
TasI AATT 3 cut(s) 729, 765, 1024
TatI WGTACW 1 cut(s) 651
TauI GCSGC 5 cut(s) 29, 61, 200, 220, 360
TfiI GAWTC 4 cut(s) 554, 803, 883, 893
Tru1I TTAA 2 cut(s) 1044, 1105
Tru9I TTAA 2 cut(s) 1044, 1105
TscAI CASTG 2 cut(s) 377, 907
TseFI GTSAC 2 cut(s) 48, 92
TseI GCWGC 5 cut(s) 142, 367, 451, 454, 470
Tsp45I GTSAC 2 cut(s) 48, 92
TspDTI ATGAA 3 cut(s) 513, 867, 1064
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 2 cut(s) 377, 907
VpaK11BI GGWCC 1 cut(s) 103
XceI RCATGY 2 cut(s) 720, 1062
XcmI CCANNNNNNNNNTGG 2 cut(s) 907, 974
XspI CTAG 1 cut(s) 861
ZraI GACGTC 2 cut(s) 39, 685
Zsp2I ATGCAT 1 cut(s) 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.