Rw4G034310

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008516
Physical Location & Seq
Forward (+)
61444562 .. 61447947
3386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034310.1

Sequence Viewer

Length: 1110 bp
ATGTCATCGGGCTCTTCAGCTACGGCGGCGGCGGCGACGGAGACGTCGGCCGAGTCACGCGCGCCGCTTCTGCTTCCCCGGCAGGGGAGCGACGGCCGTGACTTGTCGGAGCTCGGCTCCCCGACCCGCCCGGCCACGCTGGCCATGCTGCTGGGCCGGGCCACGGGCCGGCGCGGGCCGTCCATGCTGGTGAGGGAGACGGCTGCGCGTGAGCTGGACGAGCGGCGCGCCGACTGGGGTTACTCGAAGCCGGTGGTGGCGCTGGACATGATGTGGAACACGGCCTTCGTGGTGGTCTCGGTGGTGATGCTGATTCTGACCCGAGACGAGGACCCGAACACGCCGATCCGGCTCTGGATCTGCGGCTACGCGCTGCAGTGCTTTGTCCACGTGGCGCTGGTGTGGATTGAGTACCGGAGGAGGAATACGAGGAGAGGGAGGAGAGGGCCGGAGGTGCAGCAGAGCTCCGAAGCTGCCGAGGCTAATGATACTGATGATGATGAAACAGCTACGGATTTGGGGGTTCCGAGTCGTTCCAGTTTCTCTAAACGATGTGAATCAGTCAATACAATGGCATCCTTTCTCTGGTGGATAGTTGGCTTTTATTGGGTAGTCTCCGGTGGTGATTTGCTCCTGCAAAATGCTCCGCGTTTGTACTGGTTGTCTGTGGTTTTCCTGGCATTTGACGTCTTCTTTGCCATCTTTTGTGTTGTTCTGGCATGTTTGATCGGAATTGCTCTTTGTTGCTGTTTGCCATGCATCATTGCAATTCTATATGCTGTGGCAGGACAGGAAGGTGCATCCGAAGCAGATCTCAGTGTTCTTCCAAAATACAGATTTAGAATATCAGGTGATGAAAAATCTAGTGTAGGAGCAGGGAAAATGATTCCAATGGAATCAACCAGTGGGTACCTGGCAGTCGAGCGTATTCTTTTACCCGAGGATGCGGAATGTTGTATATGTCTCAGCCAATATGAGGATGGGGCAGATCTTCACACTCTTCCGTGTAACCATCACTTCCATGCTAATTGCATTGTGAAATGGCTTAAAATGAATGCAACATGTCCTCTCTGCAAATACAATATTCTCAAGGGAAGCGAACAGGTTTAA

Protein Analysis

369

Amino Acids

40.61

Weight (kDa)

5.52

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 317 - 359 3.7e-15 Ring finger domain
zf-RING_11 PF17123 317 - 345 8.2e-09 RING-like zinc finger
zf-rbx1 PF12678 317 - 359 1.1e-08 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 317 - 358 1.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 318 - 358 1.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 43
AatII GACGTC 2 cut(s) 47, 690
Acc65I GGTACC 1 cut(s) 909
AccB1I GGYRCC 1 cut(s) 909
AccBSI CCGCTC 1 cut(s) 223
AccII CGCG 7 cut(s) 60, 62, 174, 208, 228, 371, 649
AclWI GGATC 2 cut(s) 340, 365
AcoI YGGCCR 4 cut(s) 48, 94, 132, 141
AcvI CACGTG 1 cut(s) 391
AcyI GRCGYC 2 cut(s) 44, 687
AfaI GTAC 3 cut(s) 413, 656, 911
AfiI CCNNNNNNNGG 7 cut(s) 83, 84, 163, 168, 328, 585, 976
AflIII ACRYGT 1 cut(s) 1061
AjnI CCWGG 2 cut(s) 675, 912
AluBI AGCT 6 cut(s) 20, 112, 214, 465, 473, 509
AluI AGCT 6 cut(s) 20, 112, 214, 465, 473, 509
Alw21I GWGCWC 2 cut(s) 114, 467
Alw26I GTCTC 6 cut(s) 35, 191, 301, 318, 619, 968
AlwI GGATC 2 cut(s) 340, 365
Ama87I CYCGRG 2 cut(s) 321, 938
ApeKI GCWGC 5 cut(s) 148, 203, 373, 457, 473
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AscI GGCGCGCC 1 cut(s) 226
Asp718I GGTACC 1 cut(s) 909
AspLEI GCGC 9 cut(s) 62, 64, 174, 208, 228, 230, 262, 373, 397
AspS9I GGNCC 6 cut(s) 154, 159, 166, 176, 331, 446
AsuC2I CCSGG 3 cut(s) 79, 131, 158
AsuHPI GGTGA 4 cut(s) 202, 316, 635, 863
AvaI CYCGRG 2 cut(s) 321, 938
AvaII GGWCC 1 cut(s) 331
BalI TGGCCA 1 cut(s) 143
BanI GGYRCC 1 cut(s) 909
BanII GRGCYC 3 cut(s) 14, 114, 467
BbrPI CACGTG 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 682
Bbv12I GWGCWC 2 cut(s) 114, 467
BbvI GCAGC 5 cut(s) 135, 190, 360, 460, 469
BccI CCATC 3 cut(s) 707, 974, 1020
BceAI ACGGC 6 cut(s) 39, 81, 109, 163, 216, 297
BciT130I CCWGG 2 cut(s) 677, 914
BcnI CCSGG 3 cut(s) 79, 131, 158
BcoDI GTCTC 6 cut(s) 35, 191, 301, 318, 619, 968
BfaI CTAG 1 cut(s) 864
BfmI CTRYAG 1 cut(s) 374
BfoI RGCGCY 2 cut(s) 263, 398
BglI GCCNNNNNGGC 2 cut(s) 140, 349
BglII AGATCT 2 cut(s) 811, 988
Bme1390I CCNGG 5 cut(s) 79, 131, 158, 677, 914
Bme18I GGWCC 1 cut(s) 331
BmeT110I CYCGRG 2 cut(s) 321, 938
BmgT120I GGNCC 6 cut(s) 154, 159, 166, 176, 331, 446
BmiI GGNNCC 4 cut(s) 118, 333, 525, 911
BmrFI CCNGG 5 cut(s) 79, 131, 158, 677, 914
BmrI ACTGGG 1 cut(s) 244
BmsI GCATC 5 cut(s) 297, 584, 768, 809, 934
BmuI ACTGGG 1 cut(s) 244
BpiI GAAGAC 1 cut(s) 682
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpuEI CTTGAG 1 cut(s) 1073
BpuMI CCSGG 3 cut(s) 79, 131, 158
BsaAI YACGTR 1 cut(s) 391
BsaBI GATNNNNATC 1 cut(s) 556
BsaHI GRCGYC 2 cut(s) 44, 687
BsaI GGTCTC 1 cut(s) 301
BsaJI CCNNGG 4 cut(s) 77, 162, 477, 939
BsaWI WCCGGW 2 cut(s) 414, 617
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 84, 163, 168, 328, 585, 976
Bse118I RCCGGY 2 cut(s) 168, 250
Bse1I ACTGG 4 cut(s) 239, 537, 662, 903
Bse3DI GCAATG 1 cut(s) 762
Bse8I GATNNNNATC 1 cut(s) 556
BseBI CCWGG 2 cut(s) 677, 914
BseDI CCNNGG 4 cut(s) 77, 162, 477, 939
BseGI GGATG 4 cut(s) 575, 800, 949, 985
BseJI GATNNNNATC 1 cut(s) 556
BseLI CCNNNNNNNGG 7 cut(s) 83, 84, 163, 168, 328, 585, 976
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 2 cut(s) 829, 979
BseNI ACTGG 4 cut(s) 239, 537, 662, 903
BsePI GCGCGC 2 cut(s) 60, 226
BseRI GAGGAG 3 cut(s) 433, 445, 454
BseX3I CGGCCG 2 cut(s) 48, 94
BseXI GCAGC 5 cut(s) 135, 190, 360, 460, 469
BseYI CCCAGC 1 cut(s) 151
BsgI GTGCAG 1 cut(s) 476
Bsh1236I CGCG 7 cut(s) 60, 62, 174, 208, 228, 371, 649
Bsh1285I CGRYCG 2 cut(s) 51, 97
BshNI GGYRCC 1 cut(s) 909
BsiEI CGRYCG 2 cut(s) 51, 97
BsiHKAI GWGCWC 2 cut(s) 114, 467
BsiHKCI CYCGRG 2 cut(s) 321, 938
BsiSI CCGG 9 cut(s) 79, 131, 157, 169, 251, 349, 415, 449, 618
BslI CCNNNNNNNGG 7 cut(s) 83, 84, 163, 168, 328, 585, 976
BsmAI GTCTC 6 cut(s) 35, 191, 301, 318, 619, 968
BsmBI CGTCTC 3 cut(s) 35, 191, 318
BsmI GAATGC 1 cut(s) 1060
Bso31I GGTCTC 1 cut(s) 301
BsoBI CYCGRG 2 cut(s) 321, 938
Bsp1286I GDGCHC 3 cut(s) 14, 114, 467
Bsp143I GATC 5 cut(s) 345, 357, 726, 811, 988
BspCNI CTCAG 2 cut(s) 828, 978
BspFNI CGCG 7 cut(s) 60, 62, 174, 208, 228, 371, 649
BspLI GGNNCC 4 cut(s) 118, 333, 525, 911
BspMAI CTGCAG 1 cut(s) 378
BspPI GGATC 2 cut(s) 340, 365
BspQI GCTCTTC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 909
BspTNI GGTCTC 1 cut(s) 301
BsrBI CCGCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 762
BsrFI RCCGGY 2 cut(s) 168, 250
BsrI ACTGG 4 cut(s) 239, 537, 662, 903
BssAI RCCGGY 2 cut(s) 168, 250
BssECI CCNNGG 4 cut(s) 77, 162, 477, 939
BssHII GCGCGC 2 cut(s) 60, 226
BssMI GATC 5 cut(s) 345, 357, 726, 811, 988
BssNI GRCGYC 2 cut(s) 44, 687
Bst2UI CCWGG 2 cut(s) 677, 914
Bst6I CTCTTC 2 cut(s) 19, 1005
BstACI GRCGYC 2 cut(s) 44, 687
BstBAI YACGTR 1 cut(s) 391
BstC8I GCNNGC 5 cut(s) 62, 141, 170, 176, 228
BstDEI CTNAG 2 cut(s) 815, 965
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 4 cut(s) 575, 800, 949, 985
BstFNI CGCG 7 cut(s) 60, 62, 174, 208, 228, 371, 649
BstH2I RGCGCY 2 cut(s) 263, 398
BstHHI GCGC 9 cut(s) 62, 64, 174, 208, 228, 230, 262, 373, 397
BstKTI GATC 5 cut(s) 348, 360, 729, 814, 991
BstMAI GTCTC 6 cut(s) 35, 191, 301, 318, 619, 968
BstMBI GATC 5 cut(s) 345, 357, 726, 811, 988
BstMCI CGRYCG 2 cut(s) 51, 97
BstNI CCWGG 2 cut(s) 677, 914
BstNSI RCATGY 2 cut(s) 723, 1065
BstSCI CCNGG 5 cut(s) 77, 129, 156, 675, 912
BstSFI CTRYAG 1 cut(s) 374
BstUI CGCG 7 cut(s) 60, 62, 174, 208, 228, 371, 649
BstV1I GCAGC 5 cut(s) 135, 190, 360, 460, 469
BstV2I GAAGAC 1 cut(s) 682
BstX2I RGATCY 3 cut(s) 357, 811, 988
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 3 cut(s) 357, 811, 988
BstZI CGGCCG 2 cut(s) 48, 94
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 4 cut(s) 575, 800, 949, 985
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 3 cut(s) 383, 823, 910
Cac8I GCNNGC 5 cut(s) 62, 141, 170, 176, 228
CfoI GCGC 9 cut(s) 62, 64, 174, 208, 228, 230, 262, 373, 397
Cfr10I RCCGGY 2 cut(s) 168, 250
Cfr13I GGNCC 6 cut(s) 154, 159, 166, 176, 331, 446
Csp6I GTAC 3 cut(s) 412, 655, 910
CviAII CATG 7 cut(s) 145, 184, 268, 720, 756, 1022, 1062
CviQI GTAC 3 cut(s) 412, 655, 910
DdeI CTNAG 2 cut(s) 815, 965
DpnI GATC 5 cut(s) 347, 359, 728, 813, 990
DpnII GATC 5 cut(s) 345, 357, 726, 811, 988
DrdI GACNNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 1 cut(s) 43
EaeI YGGCCR 4 cut(s) 48, 94, 132, 141
EagI CGGCCG 2 cut(s) 48, 94
Eam1104I CTCTTC 2 cut(s) 19, 1005
EarI CTCTTC 2 cut(s) 19, 1005
Ecl136II GAGCTC 2 cut(s) 112, 465
EclXI CGGCCG 2 cut(s) 48, 94
Eco24I GRGCYC 3 cut(s) 14, 114, 467
Eco31I GGTCTC 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 331
Eco52I CGGCCG 2 cut(s) 48, 94
Eco53kI GAGCTC 2 cut(s) 112, 465
Eco72I CACGTG 1 cut(s) 391
Eco88I CYCGRG 2 cut(s) 321, 938
EcoICRI GAGCTC 2 cut(s) 112, 465
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 675, 912
EcoT22I ATGCAT 1 cut(s) 761
EcoT38I GRGCYC 3 cut(s) 14, 114, 467
Esp3I CGTCTC 3 cut(s) 35, 191, 318
FaeI CATG 7 cut(s) 148, 187, 271, 723, 759, 1025, 1065
FatI CATG 7 cut(s) 144, 183, 267, 719, 755, 1021, 1061
FauI CCCGC 2 cut(s) 134, 167
FokI GGATG 4 cut(s) 562, 787, 956, 992
FriOI GRGCYC 3 cut(s) 14, 114, 467
FspBI CTAG 1 cut(s) 864
GlaI GCGC 9 cut(s) 61, 63, 173, 207, 227, 229, 261, 372, 396
GsaI CCCAGC 1 cut(s) 155
HaeII RGCGCY 2 cut(s) 263, 398
HapII CCGG 9 cut(s) 79, 131, 157, 169, 251, 349, 415, 449, 618
HhaI GCGC 9 cut(s) 62, 64, 174, 208, 228, 230, 262, 373, 397
Hin1I GRCGYC 2 cut(s) 44, 687
Hin1II CATG 7 cut(s) 148, 187, 271, 723, 759, 1025, 1065
Hin6I GCGC 9 cut(s) 60, 62, 172, 206, 226, 228, 260, 371, 395
HinP1I GCGC 9 cut(s) 60, 62, 172, 206, 226, 228, 260, 371, 395
HinfI GANTC 6 cut(s) 53, 313, 529, 557, 886, 896
HpaII CCGG 9 cut(s) 79, 131, 157, 169, 251, 349, 415, 449, 618
HphI GGTGA 4 cut(s) 202, 316, 635, 863
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 6 cut(s) 109, 318, 469, 528, 731, 805
Hpy188III TCNNGA 1 cut(s) 355
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 3 cut(s) 40, 49, 95
HpyAV CCTTC 2 cut(s) 295, 788
HpyCH4IV ACGT 3 cut(s) 44, 390, 687
HpyCH4V TGCA 9 cut(s) 376, 457, 637, 759, 767, 800, 1032, 1058, 1074
HpyF3I CTNAG 2 cut(s) 815, 965
HpySE526I ACGT 3 cut(s) 44, 390, 687
Hsp92I GRCGYC 2 cut(s) 44, 687
Hsp92II CATG 7 cut(s) 148, 187, 271, 723, 759, 1025, 1065
HspAI GCGC 9 cut(s) 60, 62, 172, 206, 226, 228, 260, 371, 395
KpnI GGTACC 1 cut(s) 913
KroI GCCGGC 1 cut(s) 168
KroNI GCCGGC 1 cut(s) 170
Kzo9I GATC 5 cut(s) 345, 357, 726, 811, 988
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 7 cut(s) 87, 109, 122, 470, 636, 649, 872
Lsp1109I GCAGC 5 cut(s) 135, 190, 360, 460, 469
LweI GCATC 5 cut(s) 297, 584, 768, 809, 934
MaeI CTAG 1 cut(s) 864
MaeII ACGT 3 cut(s) 44, 390, 687
MaeIII GTNAC 4 cut(s) 54, 98, 239, 1007
MalI GATC 5 cut(s) 347, 359, 728, 813, 990
MbiI CCGCTC 1 cut(s) 223
MboI GATC 5 cut(s) 345, 357, 726, 811, 988
MboII GAAGA 5 cut(s) 6, 682, 815, 983, 992
MflI RGATCY 3 cut(s) 357, 811, 988
MhlI GDGCHC 3 cut(s) 14, 114, 467
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 3 cut(s) 732, 768, 1027
MluNI TGGCCA 1 cut(s) 143
MlyI GAGTC 2 cut(s) 62, 538
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 761
MroNI GCCGGC 1 cut(s) 168
MscI TGGCCA 1 cut(s) 143
MseI TTAA 2 cut(s) 1047, 1108
MslI CAYNNNNRTG 2 cut(s) 188, 1020
Msp20I TGGCCA 1 cut(s) 143
MspI CCGG 9 cut(s) 79, 131, 157, 169, 251, 349, 415, 449, 618
MspR9I CCNGG 5 cut(s) 79, 131, 158, 677, 914
Mva1269I GAATGC 1 cut(s) 1060
MvaI CCWGG 2 cut(s) 677, 914
MvnI CGCG 7 cut(s) 60, 62, 174, 208, 228, 371, 649
NaeI GCCGGC 1 cut(s) 170
NciI CCSGG 3 cut(s) 79, 131, 158
NdeII GATC 5 cut(s) 345, 357, 726, 811, 988
NgoMIV GCCGGC 1 cut(s) 168
NlaIII CATG 7 cut(s) 148, 187, 271, 723, 759, 1025, 1065
NlaIV GGNNCC 4 cut(s) 118, 333, 525, 911
NmeAIII GCCGAG 3 cut(s) 76, 93, 502
NmuCI GTSAC 2 cut(s) 54, 98
NsiI ATGCAT 1 cut(s) 761
NspI RCATGY 2 cut(s) 723, 1065
PalAI GGCGCGCC 1 cut(s) 226
PauI GCGCGC 2 cut(s) 60, 226
PciI ACATGT 1 cut(s) 1061
PciSI GCTCTTC 1 cut(s) 19
PctI GAATGC 1 cut(s) 1060
PdiI GCCGGC 1 cut(s) 170
PfeI GAWTC 4 cut(s) 313, 557, 886, 896
PleI GAGTC 2 cut(s) 61, 537
PmaCI CACGTG 1 cut(s) 391
PmlI CACGTG 1 cut(s) 391
PpsI GAGTC 2 cut(s) 61, 537
Ppu21I YACGTR 1 cut(s) 391
PpuMI RGGWCCY 1 cut(s) 331
PscI ACATGT 1 cut(s) 1061
Psp124BI GAGCTC 2 cut(s) 114, 467
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 675, 912
PspCI CACGTG 1 cut(s) 391
PspFI CCCAGC 1 cut(s) 151
PspGI CCWGG 2 cut(s) 675, 912
PspN4I GGNNCC 4 cut(s) 118, 333, 525, 911
PspPI GGNCC 6 cut(s) 154, 159, 166, 176, 331, 446
PspPPI RGGWCCY 1 cut(s) 331
PstI CTGCAG 1 cut(s) 378
PsuI RGATCY 3 cut(s) 357, 811, 988
PteI GCGCGC 2 cut(s) 60, 226
RsaI GTAC 3 cut(s) 413, 656, 911
RsaNI GTAC 3 cut(s) 412, 655, 910
RseI CAYNNNNRTG 2 cut(s) 188, 1020
SacI GAGCTC 2 cut(s) 114, 467
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 2 cut(s) 1047, 1108
Sau3AI GATC 5 cut(s) 345, 357, 726, 811, 988
Sau96I GGNCC 6 cut(s) 154, 159, 166, 176, 331, 446
SchI GAGTC 2 cut(s) 62, 538
ScrFI CCNGG 5 cut(s) 79, 131, 158, 677, 914
SduI GDGCHC 3 cut(s) 14, 114, 467
SfaNI GCATC 5 cut(s) 297, 584, 768, 809, 934
SfcI CTRYAG 1 cut(s) 374
SfiI GGCCNNNNNGGCC 1 cut(s) 140
SgsI GGCGCGCC 1 cut(s) 226
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 2 cut(s) 188, 1020
SmlI CTYRAG 1 cut(s) 1088
SmoI CTYRAG 1 cut(s) 1088
Sse9I AATT 3 cut(s) 732, 768, 1027
SspI AATATT 1 cut(s) 1084
SspMI CTAG 1 cut(s) 864
SstI GAGCTC 2 cut(s) 114, 467
StyD4I CCNGG 5 cut(s) 77, 129, 156, 675, 912
TaiI ACGT 3 cut(s) 47, 393, 690
TaqI TCGA 2 cut(s) 245, 921
TasI AATT 3 cut(s) 732, 768, 1027
TatI WGTACW 1 cut(s) 654
TauI GCSGC 6 cut(s) 29, 32, 35, 67, 226, 366
TfiI GAWTC 4 cut(s) 313, 557, 886, 896
Tru1I TTAA 2 cut(s) 1047, 1108
Tru9I TTAA 2 cut(s) 1047, 1108
TscAI CASTG 3 cut(s) 383, 823, 910
TseFI GTSAC 2 cut(s) 54, 98
TseI GCWGC 5 cut(s) 148, 203, 373, 457, 473
Tsp45I GTSAC 2 cut(s) 54, 98
TspDTI ATGAA 3 cut(s) 516, 870, 1067
TspGWI ACGGA 3 cut(s) 53, 527, 993
TspRI CASTG 3 cut(s) 383, 823, 910
VpaK11BI GGWCC 1 cut(s) 331
XceI RCATGY 2 cut(s) 723, 1065
XcmI CCANNNNNNNNNTGG 2 cut(s) 910, 977
XspI CTAG 1 cut(s) 864
ZraI GACGTC 2 cut(s) 45, 688
Zsp2I ATGCAT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.