Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008516

Physical Location & Seq

Genomic Coordinates Forward (+)

1342336 .. 1345714

Gene Structure 3379 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1018400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1116 bp

ATGGCGTCGGAATCTTCAGCTGCGGCGGCGGCGGAGGCCGAGTCACGTGCGCCGCTTCTTGTTTCGCGCCAGGGGAGCAGCGGCCGCGAGTCGTCCGACCTCGGCTCCCCGACCGCTCGGTCCGCCATGCTGGCGATGCTGCTGGGGCGGGCTACCGGGCGCAGGGGGCCGTCGATGCTAGTGCGGGAGACGGCGGCGCGGGAGCTGGACGAGCGGCGCGCCGACTGGGGGTACTCGAAGCCGGTCGTGGCTCTGGACATGATGTGGAACACGGCGTTCGTGGTGGTGTCCGTGGTGATGCTGTTTTGGACGAAGGACGAGCGGCCTAACACGCCGATCCGGCTGTGGATCTGCGGGTATGTGCTGCAGTGTATTGTGCACGTGGTGCTGGTGTGGGTGGAGTACCGGAGGAGGAATAATATTGCTCGGAGGCTTAGTAGGAGGAACCAGGAGGCCCAGCAGGAGGAGCACCAGGTCGATATCGATGCTGTTGATACCGACGATGAAGAAGCAGCCGGGGAATTGGCGAATTTCACTCGCTCCAGTGTCTCTAAACGATGCGAAACGGTGAATACAATGGTGTCATTTCTCTGGTGGATAGTTGGTTTCTATTGGGTAGTCTCTGGCGGTGAAGTTCTTCTGCAAAATGCTCCACATTTGTACTGGTTGGCTGTGGTTTTTCTGGCATTTGATGTCTTTTTTGCCATCTTCTGTGTTGTCCTGGCATGTTTGATTGGAGTTGCTCTTTGTTGCTGTTTGCCATGCATCATTGCAATTCTTTACGCCGTTGCAGGACAGGAAGGTGCGTCCGAAGCAGATCTCAGTATTCTGCCAAAATACAGGTTTCGAGTGAGCAGTGAGGAAAAACCTAGTGTTGGAGCTGGGAAAATGATTCCGGTAGAAACAAGCAGTGGGTACCTAGCAGTTGAGCGTGTTCTTTTATCTGAGGATGCGGAGTGTTGTATATGTCTCAGCCCGTATGAGGATGGGACAGAGCTTCATACGCTTCCCTGTAACCATCATTTCCACGCGACGTGCATTGTGAAATGGCTTAAAATGAATGCAACATGTCCTCTCTGCAAGTTCAACATTCTCAAGGGAAATGAACCGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0022414 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051603 GO:0061458 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

372

Amino Acids

41.17

Weight (kDa)

5.62

Isoelectric Point (pI)

50.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	318 - 361	6e-09	RING-H2 zinc finger domain
zf-RING_2	PF13639	319 - 361	7.9e-15	Ring finger domain
zf-RING_11	PF17123	319 - 347	3.6e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	319 - 360	1.4e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	320 - 360	1.5e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008516)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68070 AT2G01735 AT2G01735
fragaria_vesca	FvH4_4g33530 FvH4_5g19942 FvH4_6g33182
malus_domestica	MD13G1020200.v1.1 MD16G1018400.v1.1
prunus_persica	Prupe.1G333500_v2.0.a1 Prupe.1G333500_v2.0.a1
pyrus_communis	pycom16g01680
rosa_chinensis	RchiOBHm_Chr4g0443041
rosa_laevigata	RLG00000005960
rosa_multiflora	Rmu_co8232139.1_g000001 Rmu_sc0008055.1_g000003 Rmu_sc0015292.1_g000001
rosa_roxburghii	Rroxscaffold_5G00383670
rosa_rugosa	Rorug04G0344000
rosa_samantha	Rh4AG398200 Rh4BG409700 Rh4CG425200 Rh4DG404500
rosa_wichuraiana	Rw4G034310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	118
Acc65I	GGTACC	1 cut(s)	915
AccB1I	GGYRCC	1 cut(s)	915
AccBSI	CCGCTC	3 cut(s)	116, 214, 322
AccII	CGCG	5 cut(s)	67, 87, 199, 219, 1031
AclWI	GGATC	2 cut(s)	331, 356
AcoI	YGGCCR	1 cut(s)	82
AcsI	RAATTY	1 cut(s)	529
AcvI	CACGTG	2 cut(s)	47, 382
AcyI	GRCGYC	1 cut(s)	5
AdeI	CACNNNGTG	1 cut(s)	385
AfaI	GTAC	4 cut(s)	233, 404, 662, 917
AfiI	CCNNNNNNNGG	5 cut(s)	162, 228, 463, 875, 982
AflIII	ACRYGT	1 cut(s)	1067
AgeI	ACCGGT	1 cut(s)	1108
AgsI	TTSAA	1 cut(s)	1087
AjiI	CACGTC	1 cut(s)	1035
AjnI	CCWGG	4 cut(s)	69, 447, 471, 720
AluBI	AGCT	4 cut(s)	20, 205, 881, 997
AluI	AGCT	4 cut(s)	20, 205, 881, 997
Alw21I	GWGCWC	2 cut(s)	381, 471
Alw26I	GTCTC	4 cut(s)	182, 553, 625, 974
Alw44I	GTGCAC	1 cut(s)	377
AlwI	GGATC	2 cut(s)	331, 356
AoxI	GGCC	5 cut(s)	36, 82, 167, 323, 453
ApaLI	GTGCAC	1 cut(s)	377
ApeKI	GCWGC	5 cut(s)	20, 78, 139, 364, 512
ApoI	RAATTY	1 cut(s)	529
AscI	GGCGCGCC	1 cut(s)	217
AsiGI	ACCGGT	1 cut(s)	1108
Asp700I	GAANNNNTTC	1 cut(s)	636
Asp718I	GGTACC	1 cut(s)	915
AspLEI	GCGC	6 cut(s)	52, 69, 162, 199, 219, 221
AspS9I	GGNCC	3 cut(s)	120, 167, 454
AsuC2I	CCSGG	2 cut(s)	157, 517
AsuHPI	GGTGA	3 cut(s)	307, 580, 641
AvaII	GGWCC	1 cut(s)	120
BaeGI	GKGCMC	1 cut(s)	381
BanI	GGYRCC	1 cut(s)	915
BbrPI	CACGTG	2 cut(s)	47, 382
Bbv12I	GWGCWC	2 cut(s)	381, 471
BbvI	GCAGC	5 cut(s)	7, 90, 126, 351, 524
BccI	CCATC	3 cut(s)	713, 980, 1026
BceAI	ACGGC	4 cut(s)	154, 207, 288, 770
BcgI	CGANNNNNNTGC	6 cut(s)	29, 63, 163, 197, 467, 501
BciT130I	CCWGG	4 cut(s)	71, 449, 473, 722
BcnI	CCSGG	2 cut(s)	157, 517
BcoDI	GTCTC	4 cut(s)	182, 553, 625, 974
BfaI	CTAG	3 cut(s)	179, 870, 920
BfmI	CTRYAG	1 cut(s)	365
BglI	GCCNNNNNGGC	2 cut(s)	131, 340
BglII	AGATCT	1 cut(s)	817
Bme1390I	CCNGG	6 cut(s)	71, 157, 449, 473, 517, 722
Bme18I	GGWCC	1 cut(s)	120
BmgBI	CACGTC	1 cut(s)	1035
BmgT120I	GGNCC	3 cut(s)	120, 167, 454
BmiI	GGNNCC	4 cut(s)	106, 168, 446, 917
BmrFI	CCNGG	6 cut(s)	71, 157, 449, 473, 517, 722
BmrI	ACTGGG	1 cut(s)	235
BmsI	GCATC	7 cut(s)	126, 165, 288, 475, 548, 774, 940
BmuI	ACTGGG	1 cut(s)	235
BpmI	CTGGAG	1 cut(s)	526
BpuEI	CTTGAG	1 cut(s)	1079
BpuMI	CCSGG	2 cut(s)	157, 517
Bsa29I	ATCGAT	1 cut(s)	483
BsaAI	YACGTR	2 cut(s)	47, 382
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	4 cut(s)	70, 100, 291, 516
BsaWI	WCCGGW	3 cut(s)	405, 895, 1108
BsaXI	ACNNNNNCTCC	6 cut(s)	26, 56, 89, 119, 421, 451
Bsc4I	CCNNNNNNNGG	5 cut(s)	162, 228, 463, 875, 982
Bse118I	RCCGGY	2 cut(s)	241, 1108
Bse1I	ACTGG	3 cut(s)	230, 543, 668
Bse3DI	GCAATG	1 cut(s)	768
BseBI	CCWGG	4 cut(s)	71, 449, 473, 722
BseCI	ATCGAT	1 cut(s)	483
BseDI	CCNNGG	4 cut(s)	70, 100, 291, 516
BseGI	GGATG	2 cut(s)	955, 991
BseLI	CCNNNNNNNGG	5 cut(s)	162, 228, 463, 875, 982
BseMI	GCAATG	1 cut(s)	768
BseMII	CTCAG	3 cut(s)	835, 936, 985
BseNI	ACTGG	3 cut(s)	230, 543, 668
BsePI	GCGCGC	1 cut(s)	217
BseRI	GAGGAG	2 cut(s)	424, 479
BseSI	GKGCMC	1 cut(s)	381
BseX3I	CGGCCG	1 cut(s)	82
BseXI	GCAGC	5 cut(s)	7, 90, 126, 351, 524
BseYI	CCCAGC	3 cut(s)	142, 456, 881
Bsh1236I	CGCG	5 cut(s)	67, 87, 199, 219, 1031
Bsh1285I	CGRYCG	3 cut(s)	85, 114, 246
BshFI	GGCC	5 cut(s)	38, 84, 169, 325, 455
BshNI	GGYRCC	1 cut(s)	915
BshTI	ACCGGT	1 cut(s)	1108
BshVI	ATCGAT	1 cut(s)	483
BsiEI	CGRYCG	3 cut(s)	85, 114, 246
BsiHKAI	GWGCWC	2 cut(s)	381, 471
BsiSI	CCGG	7 cut(s)	156, 242, 340, 406, 516, 896, 1109
BslFI	GGGAC	1 cut(s)	1003
BslI	CCNNNNNNNGG	5 cut(s)	162, 228, 463, 875, 982
BsmAI	GTCTC	4 cut(s)	182, 553, 625, 974
BsmBI	CGTCTC	1 cut(s)	182
BsmFI	GGGAC	1 cut(s)	1003
BsmI	GAATGC	1 cut(s)	1066
BsnI	GGCC	5 cut(s)	38, 84, 169, 325, 455
Bsp1286I	GDGCHC	2 cut(s)	381, 471
Bsp143I	GATC	3 cut(s)	336, 348, 817
BspANI	GGCC	5 cut(s)	38, 84, 169, 325, 455
BspCNI	CTCAG	3 cut(s)	834, 937, 984
BspDI	ATCGAT	1 cut(s)	483
BspFNI	CGCG	5 cut(s)	67, 87, 199, 219, 1031
BspLI	GGNNCC	4 cut(s)	106, 168, 446, 917
BspMAI	CTGCAG	1 cut(s)	369
BspPI	GGATC	2 cut(s)	331, 356
BspT107I	GGYRCC	1 cut(s)	915
BsrBI	CCGCTC	3 cut(s)	116, 214, 322
BsrDI	GCAATG	1 cut(s)	768
BsrFI	RCCGGY	2 cut(s)	241, 1108
BsrI	ACTGG	3 cut(s)	230, 543, 668
BssAI	RCCGGY	2 cut(s)	241, 1108
BssECI	CCNNGG	4 cut(s)	70, 100, 291, 516
BssHII	GCGCGC	1 cut(s)	217
BssMI	GATC	3 cut(s)	336, 348, 817
BssNI	GRCGYC	1 cut(s)	5
Bst2UI	CCWGG	4 cut(s)	71, 449, 473, 722
Bst4CI	ACNGT	1 cut(s)	568
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	1 cut(s)	385
BstBAI	YACGTR	2 cut(s)	47, 382
BstC8I	GCNNGC	3 cut(s)	132, 150, 219
BstDEI	CTNAG	4 cut(s)	434, 821, 945, 971
BstDSI	CCRYGG	1 cut(s)	291
BstF5I	GGATG	2 cut(s)	955, 991
BstFNI	CGCG	5 cut(s)	67, 87, 199, 219, 1031
BstHHI	GCGC	6 cut(s)	52, 69, 162, 199, 219, 221
BstKTI	GATC	3 cut(s)	339, 351, 820
BstMAI	GTCTC	4 cut(s)	182, 553, 625, 974
BstMBI	GATC	3 cut(s)	336, 348, 817
BstMCI	CGRYCG	3 cut(s)	85, 114, 246
BstNI	CCWGG	4 cut(s)	71, 449, 473, 722
BstNSI	RCATGY	2 cut(s)	729, 1071
BstSCI	CCNGG	6 cut(s)	69, 155, 447, 471, 515, 720
BstSFI	CTRYAG	1 cut(s)	365
BstSLI	GKGCMC	1 cut(s)	381
BstUI	CGCG	5 cut(s)	67, 87, 199, 219, 1031
BstV1I	GCAGC	5 cut(s)	7, 90, 126, 351, 524
BstX2I	RGATCY	2 cut(s)	348, 817
BstYI	RGATCY	2 cut(s)	348, 817
BstZI	CGGCCG	1 cut(s)	82
Bsu15I	ATCGAT	1 cut(s)	483
BsuRI	GGCC	5 cut(s)	38, 84, 169, 325, 455
BsuTUI	ATCGAT	1 cut(s)	483
BtgI	CCRYGG	1 cut(s)	291
BtgZI	GCGATG	1 cut(s)	149
BtrI	CACGTC	1 cut(s)	1035
BtsCI	GGATG	2 cut(s)	955, 991
BtsI	GCAGTG	3 cut(s)	374, 862, 916
BtsIMutI	CAGTG	4 cut(s)	374, 550, 862, 916
Cac8I	GCNNGC	3 cut(s)	132, 150, 219
CciNI	GCGGCCGC	1 cut(s)	82
CfoI	GCGC	6 cut(s)	52, 69, 162, 199, 219, 221
Cfr10I	RCCGGY	2 cut(s)	241, 1108
Cfr13I	GGNCC	3 cut(s)	120, 167, 454
ClaI	ATCGAT	1 cut(s)	483
CpoI	CGGWCCG	1 cut(s)	120
CseI	GACGC	1 cut(s)	795
CsiI	ACCWGGT	1 cut(s)	471
Csp6I	GTAC	4 cut(s)	232, 403, 661, 916
CspAI	ACCGGT	1 cut(s)	1108
CspI	CGGWCCG	1 cut(s)	120
CviAII	CATG	5 cut(s)	127, 259, 726, 762, 1068
CviQI	GTAC	4 cut(s)	232, 403, 661, 916
DdeI	CTNAG	4 cut(s)	434, 821, 945, 971
DpnI	GATC	3 cut(s)	338, 350, 819
DpnII	GATC	3 cut(s)	336, 348, 817
DraIII	CACNNNGTG	1 cut(s)	385
DrdI	GACNNNNNNGTC	1 cut(s)	118
DseDI	GACNNNNNNGTC	1 cut(s)	118
EaeI	YGGCCR	1 cut(s)	82
EagI	CGGCCG	1 cut(s)	82
EciI	GGCGGA	2 cut(s)	47, 112
EclXI	CGGCCG	1 cut(s)	82
Eco32I	GATATC	1 cut(s)	481
Eco47I	GGWCC	1 cut(s)	120
Eco52I	CGGCCG	1 cut(s)	82
Eco72I	CACGTG	2 cut(s)	47, 382
EcoRII	CCWGG	4 cut(s)	69, 447, 471, 720
EcoRV	GATATC	1 cut(s)	481
EcoT22I	ATGCAT	1 cut(s)	767
Esp3I	CGTCTC	1 cut(s)	182
FaeI	CATG	5 cut(s)	130, 262, 729, 765, 1071
FaqI	GGGAC	1 cut(s)	1003
FatI	CATG	5 cut(s)	126, 258, 725, 761, 1067
FauI	CCCGC	4 cut(s)	141, 177, 192, 347
FokI	GGATG	2 cut(s)	962, 998
FspBI	CTAG	3 cut(s)	179, 870, 920
GlaI	GCGC	6 cut(s)	51, 68, 161, 198, 218, 220
GsaI	CCCAGC	3 cut(s)	146, 460, 885
GsuI	CTGGAG	1 cut(s)	526
HaeIII	GGCC	5 cut(s)	38, 84, 169, 325, 455
HapII	CCGG	7 cut(s)	156, 242, 340, 406, 516, 896, 1109
HgaI	GACGC	1 cut(s)	795
HhaI	GCGC	6 cut(s)	52, 69, 162, 199, 219, 221
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	5 cut(s)	130, 262, 729, 765, 1071
Hin6I	GCGC	6 cut(s)	50, 67, 160, 197, 217, 219
HinP1I	GCGC	6 cut(s)	50, 67, 160, 197, 217, 219
HinfI	GANTC	4 cut(s)	11, 41, 89, 892
HpaII	CCGG	7 cut(s)	156, 242, 340, 406, 516, 896, 1109
HphI	GGTGA	3 cut(s)	307, 580, 641
Hpy166II	GTNNAC	1 cut(s)	379
Hpy188I	TCNGA	5 cut(s)	10, 97, 429, 811, 946
Hpy188III	TCNNGA	1 cut(s)	254
Hpy8I	GTNNAC	1 cut(s)	379
Hpy99I	CGWCG	4 cut(s)	10, 175, 503, 1036
HpyAV	CCTTC	2 cut(s)	307, 794
HpyCH4III	ACNGT	1 cut(s)	568
HpyCH4IV	ACGT	3 cut(s)	46, 381, 1034
HpyCH4V	TGCA	9 cut(s)	367, 379, 643, 765, 773, 791, 1038, 1064, 1080
HpyF3I	CTNAG	4 cut(s)	434, 821, 945, 971
HpySE526I	ACGT	3 cut(s)	46, 381, 1034
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	5 cut(s)	130, 262, 729, 765, 1071
HspAI	GCGC	6 cut(s)	50, 67, 160, 197, 217, 219
KpnI	GGTACC	1 cut(s)	919
Kzo9I	GATC	3 cut(s)	336, 348, 817
LmnI	GCTCC	7 cut(s)	75, 110, 202, 466, 545, 655, 878
Lsp1109I	GCAGC	5 cut(s)	7, 90, 126, 351, 524
LweI	GCATC	7 cut(s)	126, 165, 288, 475, 548, 774, 940
MabI	ACCWGGT	1 cut(s)	471
MaeI	CTAG	3 cut(s)	179, 870, 920
MaeII	ACGT	3 cut(s)	46, 381, 1034
MaeIII	GTNAC	2 cut(s)	42, 1013
MalI	GATC	3 cut(s)	338, 350, 819
MbiI	CCGCTC	3 cut(s)	116, 214, 322
MboI	GATC	3 cut(s)	336, 348, 817
MboII	GAAGA	4 cut(s)	6, 518, 629, 700
MflI	RGATCY	2 cut(s)	348, 817
MhlI	GDGCHC	2 cut(s)	381, 471
MluCI	AATT	3 cut(s)	521, 529, 774
MlyI	GAGTC	2 cut(s)	50, 98
MmeI	TCCRAC	2 cut(s)	120, 856
Mph1103I	ATGCAT	1 cut(s)	767
MroXI	GAANNNNTTC	1 cut(s)	636
MseI	TTAA	2 cut(s)	1053, 1114
MspA1I	CMGCKG	2 cut(s)	20, 81
MspI	CCGG	7 cut(s)	156, 242, 340, 406, 516, 896, 1109
MspR9I	CCNGG	6 cut(s)	71, 157, 449, 473, 517, 722
Mva1269I	GAATGC	1 cut(s)	1066
MvaI	CCWGG	4 cut(s)	71, 449, 473, 722
MvnI	CGCG	5 cut(s)	67, 87, 199, 219, 1031
NciI	CCSGG	2 cut(s)	157, 517
NdeII	GATC	3 cut(s)	336, 348, 817
NlaIII	CATG	5 cut(s)	130, 262, 729, 765, 1071
NlaIV	GGNNCC	4 cut(s)	106, 168, 446, 917
NmeAIII	GCCGAG	2 cut(s)	64, 81
NmuCI	GTSAC	1 cut(s)	42
NotI	GCGGCCGC	1 cut(s)	82
NsiI	ATGCAT	1 cut(s)	767
NspI	RCATGY	2 cut(s)	729, 1071
PalAI	GGCGCGCC	1 cut(s)	217
PauI	GCGCGC	1 cut(s)	217
PciI	ACATGT	1 cut(s)	1067
PctI	GAATGC	1 cut(s)	1066
PdmI	GAANNNNTTC	1 cut(s)	636
PfeI	GAWTC	2 cut(s)	11, 892
PinAI	ACCGGT	1 cut(s)	1108
PleI	GAGTC	2 cut(s)	49, 97
PmaCI	CACGTG	2 cut(s)	47, 382
PmlI	CACGTG	2 cut(s)	47, 382
PpsI	GAGTC	2 cut(s)	49, 97
Ppu21I	YACGTR	2 cut(s)	47, 382
PscI	ACATGT	1 cut(s)	1067
Psp6I	CCWGG	4 cut(s)	69, 447, 471, 720
PspCI	CACGTG	2 cut(s)	47, 382
PspFI	CCCAGC	3 cut(s)	142, 456, 881
PspGI	CCWGG	4 cut(s)	69, 447, 471, 720
PspN4I	GGNNCC	4 cut(s)	106, 168, 446, 917
PspPI	GGNCC	3 cut(s)	120, 167, 454
PstI	CTGCAG	1 cut(s)	369
PsuI	RGATCY	2 cut(s)	348, 817
PteI	GCGCGC	1 cut(s)	217
PvuII	CAGCTG	1 cut(s)	20
RsaI	GTAC	4 cut(s)	233, 404, 662, 917
RsaNI	GTAC	4 cut(s)	232, 403, 661, 916
Rsr2I	CGGWCCG	1 cut(s)	120
RsrII	CGGWCCG	1 cut(s)	120
SaqAI	TTAA	2 cut(s)	1053, 1114
Sau3AI	GATC	3 cut(s)	336, 348, 817
Sau96I	GGNCC	3 cut(s)	120, 167, 454
SchI	GAGTC	2 cut(s)	50, 98
ScrFI	CCNGG	6 cut(s)	71, 157, 449, 473, 517, 722
SduI	GDGCHC	2 cut(s)	381, 471
SexAI	ACCWGGT	1 cut(s)	471
SfaNI	GCATC	7 cut(s)	126, 165, 288, 475, 548, 774, 940
SfcI	CTRYAG	1 cut(s)	365
SgsI	GGCGCGCC	1 cut(s)	217
SinI	GGWCC	1 cut(s)	120
SmlI	CTYRAG	1 cut(s)	1094
SmoI	CTYRAG	1 cut(s)	1094
Sse9I	AATT	3 cut(s)	521, 529, 774
SspI	AATATT	1 cut(s)	421
SspMI	CTAG	3 cut(s)	179, 870, 920
StyD4I	CCNGG	6 cut(s)	69, 155, 447, 471, 515, 720
TaaI	ACNGT	1 cut(s)	568
TaiI	ACGT	3 cut(s)	49, 384, 1037
TaqI	TCGA	5 cut(s)	173, 236, 477, 483, 847
TaqII	GACCGA	1 cut(s)	108
TasI	AATT	3 cut(s)	521, 529, 774
TatI	WGTACW	1 cut(s)	660
TauI	GCSGC	9 cut(s)	26, 29, 32, 55, 84, 87, 197, 217, 325
TfiI	GAWTC	2 cut(s)	11, 892
Tru1I	TTAA	2 cut(s)	1053, 1114
Tru9I	TTAA	2 cut(s)	1053, 1114
TscAI	CASTG	4 cut(s)	374, 550, 862, 916
TseFI	GTSAC	1 cut(s)	42
TseI	GCWGC	5 cut(s)	20, 78, 139, 364, 512
Tsp45I	GTSAC	1 cut(s)	42
TspDTI	ATGAA	3 cut(s)	519, 989, 1073
TspGWI	ACGGA	1 cut(s)	280
TspRI	CASTG	4 cut(s)	374, 550, 862, 916
VneI	GTGCAC	1 cut(s)	377
VpaK11BI	GGWCC	1 cut(s)	120
XapI	RAATTY	1 cut(s)	529
XceI	RCATGY	2 cut(s)	729, 1071
XcmI	CCANNNNNNNNNTGG	1 cut(s)	660
XmnI	GAANNNNTTC	1 cut(s)	636
XspI	CTAG	3 cut(s)	179, 870, 920
Zsp2I	ATGCAT	1 cut(s)	767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.