AT2G01735

E3 ubiquitin protein ligase RIE1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008516
Physical Location & Seq
Forward (+)
324365 .. 326094
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G01735.1

Sequence Viewer

Length: 1080 bp
ATGTCATCGTATTCTTCAGATTCCACGGCGGCGCGTGATCAACATGCGCCTCTACTCCGTCCACGACACGACGGCTCTTTTTCTTCTTCTTCTTCATCAGCCAGACCTACAGCTCTCGCCGTTCTATTAGGACGGATCACCGGCCACCGAGCACCGTCGATGCTGGTTAGAGAAACAGCGGCGCGTGCTCTCGAGGAGAGACGAATCGATTGGGGTTACTCGAAGCCTGTAGTTGCTGCTGATATACTATGGAACGCTGCTTTAGTTCTTGCGTCAGCGGTTATGCTTGTCGGTACCGTTGAAGAAAGACCTAATGAACCGATTAGGGTTTGGATCTGTGTGTATGGGTTACAGTGTTTGTTCCATGTGGTTTTGGTTTGGTCTGAGTATTGGAGAAGAAACTCAACTCGTAGAGCTAGGGATTTGGAGTCTTATGATCATGAAGATTACAACATTGAGTATGATTATGAACAAGACAGTGATGACAATTCAACAACTTACAGTTTTGTGAAGAGATGTGAGTCGATAAACACAGTGATATCATTCATATGGTGGATAATTGGATTCTACTGGGTTGTTGAAGGTGGTGATAAGCTTTTAGGAGAAGCTCCTAATCTTTACTGGTTATCGGTGATTTTCCTGGCGATTGACGTCTTCTTTGCTGTTTTCTGTGTTGTTTTGGCTTGCCTTGTTGGAATAGCTCTTTGTTGCTGTCTTCCTTGCATAATTGCTCTTCTTTATGCCGTTGCTGGAACGGAAGGTGTATCGGAGGCGGAGCTCGGTGTTCTTCCCTTGTACAAATTTAAGGCTTTCCATAGCAATGAGAAGAACATTACTGGACCTGGTAAAATGGTTCCTATACCGATCAATGGCTTATGCTTAGCAACTGAAAGAACACTGCTTGCTGAGGATGCGGATTGTTGCATATGTCTGAGTTCATATGAGGATGGCGCAGAGCTTCATGCTCTTCCTTGTAACCACCATTTTCATTCGACTTGTATTGTGAAATGGCTTAAGATGAGAGCAACATGCCCTCTTTGCAAATACAACATTCTTAAAGGAACCACTGATCAATCTTGA

Protein Analysis

359

Amino Acids

40.1

Weight (kDa)

5.6

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 306 - 348 2.3e-14 Ring finger domain
zf-RING_11 PF17123 306 - 334 7.3e-09 RING-like zinc finger
zf-rbx1 PF12678 306 - 348 1.2e-08 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 307 - 347 7.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 307 - 347 3.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 654
Acc65I GGTACC 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 293
AccII CGCG 2 cut(s) 34, 184
AciI CCGC 5 cut(s) 29, 179, 278, 773, 914
AclWI GGATC 2 cut(s) 143, 341
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 800
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 2 cut(s) 295, 797
AfiI CCNNNNNNNGG 1 cut(s) 869
AflII CTTAAG 1 cut(s) 1013
AgsI TTSAA 3 cut(s) 302, 492, 581
AjnI CCWGG 2 cut(s) 639, 841
AluBI AGCT 7 cut(s) 113, 416, 595, 608, 701, 778, 958
AluI AGCT 7 cut(s) 113, 416, 595, 608, 701, 778, 958
Alw21I GWGCWC 3 cut(s) 154, 190, 780
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 2 cut(s) 143, 341
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 2 cut(s) 236, 257
ApoI RAATTY 1 cut(s) 800
ArsI GACNNNNNNTTYG 2 cut(s) 641, 673
Asp718I GGTACC 1 cut(s) 293
AspLEI GCGC 4 cut(s) 34, 49, 184, 953
AspS9I GGNCC 1 cut(s) 839
AsuHPI GGTGA 3 cut(s) 130, 599, 643
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 839
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 780
BbsI GAAGAC 2 cut(s) 646, 707
Bbv12I GWGCWC 3 cut(s) 154, 190, 780
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 2 cut(s) 223, 244
BccI CCATC 1 cut(s) 941
BceAI ACGGC 4 cut(s) 42, 88, 104, 728
BciT130I CCWGG 2 cut(s) 641, 843
BclI TGATCA 3 cut(s) 37, 436, 1069
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 417
BfmI CTRYAG 2 cut(s) 108, 228
BfrI CTTAAG 1 cut(s) 1013
BisI GCNGC 4 cut(s) 30, 180, 237, 258
BlpI GCTNAGC 1 cut(s) 880
BlsI GCNGC 4 cut(s) 31, 181, 238, 259
Bme1390I CCNGG 2 cut(s) 641, 843
Bme18I GGWCC 1 cut(s) 839
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 839
BmiI GGNNCC 3 cut(s) 295, 855, 1063
BmrFI CCNGG 2 cut(s) 641, 843
BmrI ACTGGG 1 cut(s) 580
BmsI GCATC 2 cut(s) 150, 901
BmuI ACTGGG 1 cut(s) 580
BpiI GAAGAC 2 cut(s) 646, 707
Bpu10I CCTNAGC 1 cut(s) 906
Bpu1102I GCTNAGC 1 cut(s) 880
Bsa29I ATCGAT 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 651
BsaJI CCNNGG 1 cut(s) 24
Bsc4I CCNNNNNNNGG 1 cut(s) 869
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 3 cut(s) 575, 626, 841
Bse3DI GCAATG 1 cut(s) 826
BseBI CCWGG 2 cut(s) 641, 843
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 2 cut(s) 916, 952
BseLI CCNNNNNNNGG 1 cut(s) 869
BseMI GCAATG 1 cut(s) 826
BseMII CTCAG 3 cut(s) 375, 897, 923
BseNI ACTGG 3 cut(s) 575, 626, 841
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 2 cut(s) 223, 244
Bsh1236I CGCG 2 cut(s) 34, 184
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 3 cut(s) 154, 190, 780
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 869
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsnI GGCC 1 cut(s) 144
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 3 cut(s) 154, 190, 780
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 6 cut(s) 37, 135, 333, 436, 864, 1069
Bsp1720I GCTNAGC 1 cut(s) 880
BspACI CCGC 5 cut(s) 29, 179, 278, 773, 914
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 3 cut(s) 376, 898, 924
BspDI ATCGAT 1 cut(s) 207
BspFNI CGCG 2 cut(s) 34, 184
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 295, 855, 1063
BspPI GGATC 2 cut(s) 143, 341
BspQI GCTCTTC 2 cut(s) 738, 972
BspT107I GGYRCC 1 cut(s) 293
BspTI CTTAAG 1 cut(s) 1013
BsrDI GCAATG 1 cut(s) 826
BsrFI RCCGGY 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 3 cut(s) 575, 626, 841
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 6 cut(s) 37, 135, 333, 436, 864, 1069
BssNI GRCGYC 1 cut(s) 651
Bst2UI CCWGG 2 cut(s) 641, 843
Bst4CI ACNGT 6 cut(s) 156, 298, 354, 479, 503, 535
Bst6I CTCTTC 3 cut(s) 506, 738, 972
BstACI GRCGYC 1 cut(s) 651
BstAFI CTTAAG 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 795
BstC8I GCNNGC 3 cut(s) 186, 685, 903
BstDEI CTNAG 4 cut(s) 384, 880, 906, 932
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 916, 952
BstFNI CGCG 2 cut(s) 34, 184
BstHHI GCGC 4 cut(s) 34, 49, 184, 953
BstKTI GATC 6 cut(s) 40, 138, 336, 439, 867, 1072
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 6 cut(s) 37, 135, 333, 436, 864, 1069
BstMWI GCNNNNNNNGC 3 cut(s) 185, 911, 1038
BstNI CCWGG 2 cut(s) 641, 843
BstNSI RCATGY 2 cut(s) 47, 1032
BstSCI CCNGG 2 cut(s) 639, 841
BstSFI CTRYAG 2 cut(s) 108, 228
BstUI CGCG 2 cut(s) 34, 184
BstV1I GCAGC 2 cut(s) 223, 244
BstV2I GAAGAC 2 cut(s) 646, 707
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 1 cut(s) 144
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 2 cut(s) 916, 952
BtsI GCAGTG 1 cut(s) 896
BtsIMutI CAGTG 5 cut(s) 359, 484, 540, 896, 1065
Cac8I GCNNGC 3 cut(s) 186, 685, 903
CciI TCATGA 1 cut(s) 439
CfoI GCGC 4 cut(s) 34, 49, 184, 953
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 839
ClaI ATCGAT 1 cut(s) 207
CseI GACGC 1 cut(s) 261
CsiI ACCWGGT 1 cut(s) 841
Csp6I GTAC 2 cut(s) 294, 796
CviAII CATG 5 cut(s) 44, 365, 440, 962, 1029
CviQI GTAC 2 cut(s) 294, 796
DdeI CTNAG 4 cut(s) 384, 880, 906, 932
DpnI GATC 6 cut(s) 39, 137, 335, 438, 866, 1071
DpnII GATC 6 cut(s) 37, 135, 333, 436, 864, 1069
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 3 cut(s) 506, 738, 972
EarI CTCTTC 3 cut(s) 506, 738, 972
EciI GGCGGA 1 cut(s) 788
Ecl136II GAGCTC 1 cut(s) 778
Eco24I GRGCYC 1 cut(s) 780
Eco32I GATATC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 839
Eco53kI GAGCTC 1 cut(s) 778
Eco88I CYCGRG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 639, 841
EcoRV GATATC 1 cut(s) 540
EcoT38I GRGCYC 1 cut(s) 780
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 5 cut(s) 47, 368, 443, 965, 1032
FatI CATG 5 cut(s) 43, 364, 439, 961, 1028
FauNDI CATATG 3 cut(s) 548, 926, 940
FbaI TGATCA 3 cut(s) 37, 436, 1069
Fnu4HI GCNGC 4 cut(s) 30, 180, 237, 258
FokI GGATG 2 cut(s) 923, 959
FriOI GRGCYC 1 cut(s) 780
Fsp4HI GCNGC 4 cut(s) 30, 180, 237, 258
FspBI CTAG 1 cut(s) 417
GlaI GCGC 4 cut(s) 33, 48, 183, 952
GluI GCNGC 4 cut(s) 30, 180, 237, 258
HaeIII GGCC 1 cut(s) 144
HapII CCGG 1 cut(s) 141
HgaI GACGC 1 cut(s) 261
HhaI GCGC 4 cut(s) 34, 49, 184, 953
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 5 cut(s) 47, 368, 443, 965, 1032
Hin6I GCGC 4 cut(s) 32, 47, 182, 951
HinP1I GCGC 4 cut(s) 32, 47, 182, 951
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 5 cut(s) 20, 204, 428, 521, 564
HpaII CCGG 1 cut(s) 141
HphI GGTGA 3 cut(s) 130, 599, 643
Hpy166II GTNNAC 1 cut(s) 62
Hpy188I TCNGA 4 cut(s) 19, 385, 769, 933
Hpy188III TCNNGA 3 cut(s) 191, 440, 1077
Hpy8I GTNNAC 1 cut(s) 62
Hpy99I CGWCG 2 cut(s) 74, 160
HpyAV CCTTC 2 cut(s) 575, 752
HpyCH4III ACNGT 6 cut(s) 156, 298, 354, 479, 503, 535
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 3 cut(s) 723, 924, 1041
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 911, 1038
HpyF3I CTNAG 4 cut(s) 384, 880, 906, 932
HpySE526I ACGT 1 cut(s) 651
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 5 cut(s) 47, 368, 443, 965, 1032
HspAI GCGC 4 cut(s) 32, 47, 182, 951
KpnI GGTACC 1 cut(s) 297
Ksp22I TGATCA 3 cut(s) 37, 436, 1069
Kzo9I GATC 6 cut(s) 37, 135, 333, 436, 864, 1069
LguI GCTCTTC 2 cut(s) 738, 972
LmnI GCTCC 2 cut(s) 613, 775
Lsp1109I GCAGC 2 cut(s) 223, 244
LweI GCATC 2 cut(s) 150, 901
MabI ACCWGGT 1 cut(s) 841
MaeI CTAG 1 cut(s) 417
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 3 cut(s) 215, 348, 974
MalI GATC 6 cut(s) 39, 137, 335, 438, 866, 1071
MboI GATC 6 cut(s) 37, 135, 333, 436, 864, 1069
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 3 cut(s) 154, 190, 780
MluCI AATT 4 cut(s) 487, 558, 726, 800
MlyI GAGTC 2 cut(s) 437, 530
MmeI TCCRAC 1 cut(s) 673
MnlI CCTC 6 cut(s) 60, 187, 763, 901, 937, 1044
MseI TTAA 3 cut(s) 804, 1014, 1056
MslI CAYNNNNRTG 2 cut(s) 547, 819
MspA1I CMGCKG 2 cut(s) 179, 278
MspCI CTTAAG 1 cut(s) 1013
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 2 cut(s) 641, 843
MvaI CCWGG 2 cut(s) 641, 843
MvnI CGCG 2 cut(s) 34, 184
MwoI GCNNNNNNNGC 3 cut(s) 185, 911, 1038
NdeI CATATG 3 cut(s) 548, 926, 940
NdeII GATC 6 cut(s) 37, 135, 333, 436, 864, 1069
NlaIII CATG 5 cut(s) 47, 368, 443, 965, 1032
NlaIV GGNNCC 3 cut(s) 295, 855, 1063
NspI RCATGY 2 cut(s) 47, 1032
PaeR7I CTCGAG 1 cut(s) 191
PagI TCATGA 1 cut(s) 439
PciSI GCTCTTC 2 cut(s) 738, 972
PfeI GAWTC 3 cut(s) 20, 204, 564
PkrI GCNGC 4 cut(s) 31, 181, 238, 259
PleI GAGTC 2 cut(s) 436, 529
PpsI GAGTC 2 cut(s) 436, 529
Psp124BI GAGCTC 1 cut(s) 780
Psp6I CCWGG 2 cut(s) 639, 841
PspGI CCWGG 2 cut(s) 639, 841
PspN4I GGNNCC 3 cut(s) 295, 855, 1063
PspPI GGNCC 1 cut(s) 839
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 2 cut(s) 295, 797
RsaNI GTAC 2 cut(s) 294, 796
RseI CAYNNNNRTG 2 cut(s) 547, 819
SacI GAGCTC 1 cut(s) 780
SapI GCTCTTC 2 cut(s) 738, 972
SaqAI TTAA 3 cut(s) 804, 1014, 1056
SatI GCNGC 4 cut(s) 30, 180, 237, 258
Sau3AI GATC 6 cut(s) 37, 135, 333, 436, 864, 1069
Sau96I GGNCC 1 cut(s) 839
SchI GAGTC 2 cut(s) 437, 530
ScrFI CCNGG 2 cut(s) 641, 843
SduI GDGCHC 3 cut(s) 154, 190, 780
SexAI ACCWGGT 1 cut(s) 841
SfaNI GCATC 2 cut(s) 150, 901
SfcI CTRYAG 2 cut(s) 108, 228
Sfr274I CTCGAG 1 cut(s) 191
SinI GGWCC 1 cut(s) 839
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 2 cut(s) 547, 819
SmlI CTYRAG 2 cut(s) 191, 1013
SmoI CTYRAG 2 cut(s) 191, 1013
Sse9I AATT 4 cut(s) 487, 558, 726, 800
SsiI CCGC 5 cut(s) 29, 179, 278, 773, 914
SspMI CTAG 1 cut(s) 417
SstI GAGCTC 1 cut(s) 780
StyD4I CCNGG 2 cut(s) 639, 841
TaaI ACNGT 6 cut(s) 156, 298, 354, 479, 503, 535
TaiI ACGT 1 cut(s) 654
TaqI TCGA 6 cut(s) 158, 192, 207, 221, 524, 992
TasI AATT 4 cut(s) 487, 558, 726, 800
TatI WGTACW 1 cut(s) 795
TauI GCSGC 2 cut(s) 32, 182
TfiI GAWTC 3 cut(s) 20, 204, 564
Tru1I TTAA 3 cut(s) 804, 1014, 1056
Tru9I TTAA 3 cut(s) 804, 1014, 1056
TscAI CASTG 5 cut(s) 359, 484, 540, 903, 1072
TseI GCWGC 2 cut(s) 236, 257
TspDTI ATGAA 8 cut(s) 84, 330, 456, 483, 535, 927, 950, 977
TspGWI ACGGA 3 cut(s) 47, 148, 770
TspRI CASTG 5 cut(s) 359, 484, 540, 903, 1072
Vha464I CTTAAG 1 cut(s) 1013
VpaK11BI GGWCC 1 cut(s) 839
XapI RAATTY 1 cut(s) 800
XceI RCATGY 2 cut(s) 47, 1032
XhoI CTCGAG 1 cut(s) 191
XspI CTAG 1 cut(s) 417
ZraI GACGTC 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.