AT1G69800

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003883
Physical Location & Seq
Reverse (-)
26274195 .. 26276336
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69800.3

Sequence Viewer

Length: 1344 bp
ATGGCGGGAAGTGAAGCGGTAGAAGATAAGGAGATAAAATCGGCGGTTTCAAGCTGTGAAGCGTATTTCGAGAAAGTACAATCAAGGAAGAATCTACCCAAATCTCTTCAAGAAACCCTAAACTCTGCTTTCGCCGGAATCCCAGTCTCCTCCTTCCCTCAAGTCCCCGGCGGCAGAGTGATAGAGATTCAAGCGGAAACACCTGTATCAGAAGCAGTAAAGATCTTATCAGATAGCAAGATTCTATCAGCTCCTGTGATTAACACAGATCACGAGAGTTCTTTAGATTGGAGAGAAAGATATTTAGGAATCATTGACTACTCTTCAATCATCTTATGGGTGCTAGAGAGTGCTGAACTCGCTGCAATCGCGTTGTCCGCTACGTCAGCAACTGCTGCTGGTGTTGGTGCTGGAGCTGTTGGAGCTCTTGGTGTTGCAGCGCTCGGGATGACCGGTCCGGTTGCTGCTGCGGGGCTAGCCGCAGCTGCGGTTGGAGCCGCGGTTGCTGGTGGTGTTGCAGCTGAACGTGGGATTGGAAAAGATGCTCCAACTGCTGCTGATAAGTTGGGGAAAGATTTCTATGAAGTTATTCTTCAAGAAGAGCCTTTTAAATCAACCACTGTTAGAACAATACTTAAGTCGTTTAGATGGGCTCCTTTTCTTCCGGTTTCAACAGAGAGTTCGATGTTGAGTGTTATGTTGTTACTCTCGAAATATCGGTTAAGAAATGTGCCGGTTATTAAAACAGGAGAACCGGATATCAAGAACTACGTTACTCAATCTGCAGTTGTTCATGGTCTTGAAGGTTGCAAAGGTAGAGATTGGTTTGATCACATTTCCGCTCTCCCGATTTCTGATCTCGGCCTCCCGTTTATGTCACCCAATGAGGTTATTAGCATTGAGAGTGAGGAACTTATCCTTGAAGCATTTAAAAGGATGAGAGACAACAACATTGGTGGTCTCCCTGTAGTTGAAGGGTTGAATAAGAAGATTGTTGGTAACATTAGCATGAGAGACATTAGATATTTGCTTTTACAACCTGAAGTCTTTTCCAATTTCAGACAACTCACGGTGAAGAGTTTCGCGACAAAGATTGCTACAGCGGGAGAGGAATACGGTTTAGCGATTCCTGCAATAACTTGTAGGCCTGATTCGACTTTAGGGAGTGTTATAAACAGTTTGGCTTCGAGGTCGGTGCATCGGGTTTATGTAGCGGCTGGAGATGAGAATGAGCTTTATGGGGTTATAACACTGAGGGATGTGATCTCTTGTTTCGTATCAGAACCTCCAAATTACTTTGAGAACTGTCTCGGTTTCTCGGTTAAGGAAATGCTTAACCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

447

Amino Acids

47.82

Weight (kDa)

5.25

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBS PF00571 292 - 344 1.6e-09 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1172, 1247
AccBSI CCGCTC 1 cut(s) 842
AccII CGCG 3 cut(s) 371, 500, 1085
AcuI CTGAAG 1 cut(s) 1062
AfaI GTAC 1 cut(s) 78
AfeI AGCGCT 1 cut(s) 441
AflII CTTAAG 1 cut(s) 635
AgeI ACCGGT 1 cut(s) 452
AjuI GAANNNNNNNTTGG 2 cut(s) 516, 548
AluBI AGCT 7 cut(s) 54, 251, 416, 425, 485, 521, 1234
AluI AGCT 7 cut(s) 54, 251, 416, 425, 485, 521, 1234
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 5 cut(s) 151, 936, 965, 1008, 1313
AlwNI CAGNNNCTG 2 cut(s) 254, 392
Ama87I CYCGRG 1 cut(s) 443
Aor51HI AGCGCT 1 cut(s) 441
AoxI GGCC 2 cut(s) 862, 1145
ApeKI GCWGC 9 cut(s) 362, 395, 437, 464, 467, 482, 485, 518, 554
AsiGI ACCGGT 1 cut(s) 452
Asp700I GAANNNNTTC 3 cut(s) 575, 588, 1079
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 455
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 870, 1084
AsuNHI GCTAGC 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 1 cut(s) 455
BaeI ACNNNNGTAYC 2 cut(s) 189, 222
BanII GRGCYC 2 cut(s) 427, 655
BauI CACGAG 1 cut(s) 272
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 9 cut(s) 349, 382, 449, 451, 454, 472, 494, 530, 541
BccI CCATC 1 cut(s) 642
BcgI CGANNNNNNTGC 2 cut(s) 1177, 1211
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 5 cut(s) 151, 936, 965, 1008, 1313
BfaI CTAG 2 cut(s) 344, 476
BfmI CTRYAG 3 cut(s) 783, 966, 1098
BfoI RGCGCY 1 cut(s) 443
BfrI CTTAAG 1 cut(s) 635
BglII AGATCT 1 cut(s) 222
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 1 cut(s) 455
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 2 cut(s) 496, 654
BmrFI CCNGG 1 cut(s) 168
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 2 cut(s) 532, 1207
BmtI GCTAGC 1 cut(s) 479
BmuI ACTGGG 1 cut(s) 137
BpmI CTGGAG 2 cut(s) 432, 1239
BpuEI CTTGAG 1 cut(s) 144
BpuMI CCSGG 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 965
BsaJI CCNNGG 2 cut(s) 166, 498
BsaWI WCCGGW 4 cut(s) 452, 457, 664, 754
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bse118I RCCGGY 2 cut(s) 452, 733
Bse1I ACTGG 1 cut(s) 143
BseDI CCNNGG 2 cut(s) 166, 498
BseGI GGATG 3 cut(s) 453, 942, 1264
BseMII CTCAG 1 cut(s) 1244
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 9 cut(s) 349, 382, 449, 451, 454, 472, 494, 530, 541
Bsh1236I CGCG 3 cut(s) 371, 500, 1085
BshFI GGCC 2 cut(s) 864, 1147
BshTI ACCGGT 1 cut(s) 452
BsiHKAI GWGCWC 1 cut(s) 427
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 7 cut(s) 135, 168, 453, 458, 665, 734, 755
BslFI GGGAC 1 cut(s) 149
BsmAI GTCTC 5 cut(s) 151, 936, 965, 1008, 1313
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 2 cut(s) 864, 1147
Bso31I GGTCTC 1 cut(s) 965
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 2 cut(s) 427, 655
Bsp143I GATC 5 cut(s) 222, 268, 829, 856, 1263
Bsp68I TCGCGA 1 cut(s) 1085
BspANI GGCC 2 cut(s) 864, 1147
BspCNI CTCAG 1 cut(s) 1245
BspFNI CGCG 3 cut(s) 371, 500, 1085
BspLI GGNNCC 2 cut(s) 496, 654
BspMAI CTGCAG 1 cut(s) 787
BspOI GCTAGC 1 cut(s) 479
BspQI GCTCTTC 1 cut(s) 594
BspTI CTTAAG 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 965
BsrBI CCGCTC 1 cut(s) 842
BsrFI RCCGGY 2 cut(s) 452, 733
BsrI ACTGG 1 cut(s) 143
BssAI RCCGGY 2 cut(s) 452, 733
BssECI CCNNGG 2 cut(s) 166, 498
BssMI GATC 5 cut(s) 222, 268, 829, 856, 1263
BssSI CACGAG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 272
Bst4CI ACNGT 5 cut(s) 622, 1072, 1118, 1178, 1307
Bst6I CTCTTC 4 cut(s) 111, 328, 594, 1070
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 1 cut(s) 1253
BstDSI CCRYGG 1 cut(s) 498
BstF5I GGATG 3 cut(s) 453, 942, 1264
BstFNI CGCG 3 cut(s) 371, 500, 1085
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 5 cut(s) 225, 271, 832, 859, 1266
BstMAI GTCTC 5 cut(s) 151, 936, 965, 1008, 1313
BstMBI GATC 5 cut(s) 222, 268, 829, 856, 1263
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 3 cut(s) 783, 966, 1098
BstUI CGCG 3 cut(s) 371, 500, 1085
BstV1I GCAGC 9 cut(s) 349, 382, 449, 451, 454, 472, 494, 530, 541
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuRI GGCC 2 cut(s) 864, 1147
BtgI CCRYGG 1 cut(s) 498
BtsCI GGATG 3 cut(s) 453, 942, 1264
BtsIMutI CAGTG 2 cut(s) 618, 1250
BtuMI TCGCGA 1 cut(s) 1085
Cac8I GCNNGC 1 cut(s) 477
CaiI CAGNNNCTG 2 cut(s) 254, 392
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 2 cut(s) 452, 733
Cfr13I GGNCC 1 cut(s) 455
Cfr42I CCGCGG 1 cut(s) 501
CpoI CGGWCCG 1 cut(s) 455
Csp6I GTAC 1 cut(s) 77
CspAI ACCGGT 1 cut(s) 452
CspCI CAANNNNNGTGG 2 cut(s) 937, 972
CspI CGGWCCG 1 cut(s) 455
CviAII CATG 2 cut(s) 794, 1009
CviQI GTAC 1 cut(s) 77
DdeI CTNAG 1 cut(s) 1253
DpnI GATC 5 cut(s) 224, 270, 831, 858, 1265
DpnII GATC 5 cut(s) 222, 268, 829, 856, 1263
DraI TTTAAA 2 cut(s) 610, 931
Eam1104I CTCTTC 4 cut(s) 111, 328, 594, 1070
EarI CTCTTC 4 cut(s) 111, 328, 594, 1070
Ecl136II GAGCTC 1 cut(s) 425
Eco147I AGGCCT 1 cut(s) 1147
Eco24I GRGCYC 2 cut(s) 427, 655
Eco31I GGTCTC 1 cut(s) 965
Eco32I GATATC 1 cut(s) 760
Eco47I GGWCC 1 cut(s) 455
Eco47III AGCGCT 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 1062
Eco88I CYCGRG 1 cut(s) 443
EcoICRI GAGCTC 1 cut(s) 425
EcoRV GATATC 1 cut(s) 760
EcoT38I GRGCYC 2 cut(s) 427, 655
FaeI CATG 2 cut(s) 797, 1012
FalI AAGNNNNNCTT 2 cut(s) 576, 608
FaqI GGGAC 1 cut(s) 149
FatI CATG 2 cut(s) 793, 1008
FauI CCCGC 2 cut(s) 463, 1096
FbaI TGATCA 1 cut(s) 829
FokI GGATG 3 cut(s) 460, 949, 1271
FriOI GRGCYC 2 cut(s) 427, 655
FspBI CTAG 2 cut(s) 344, 476
GlaI GCGC 1 cut(s) 441
GsuI CTGGAG 2 cut(s) 432, 1239
HaeII RGCGCY 1 cut(s) 443
HaeIII GGCC 2 cut(s) 864, 1147
HapII CCGG 7 cut(s) 135, 168, 453, 458, 665, 734, 755
HhaI GCGC 1 cut(s) 442
Hin1II CATG 2 cut(s) 797, 1012
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HinfI GANTC 7 cut(s) 91, 138, 187, 241, 309, 1126, 1151
HpaII CCGG 7 cut(s) 135, 168, 453, 458, 665, 734, 755
HphI GGTGA 2 cut(s) 870, 1084
Hpy188I TCNGA 5 cut(s) 211, 232, 856, 1061, 1282
HpyAV CCTTC 3 cut(s) 163, 797, 968
HpyCH4III ACNGT 5 cut(s) 622, 1072, 1118, 1178, 1307
HpyCH4IV ACGT 3 cut(s) 383, 526, 771
HpyCH4V TGCA 7 cut(s) 365, 437, 518, 785, 810, 1133, 1198
HpyF3I CTNAG 1 cut(s) 1253
HpySE526I ACGT 3 cut(s) 383, 526, 771
Hsp92II CATG 2 cut(s) 797, 1012
HspAI GCGC 1 cut(s) 440
Ksp22I TGATCA 1 cut(s) 829
KspI CCGCGG 1 cut(s) 501
Kzo9I GATC 5 cut(s) 222, 268, 829, 856, 1263
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 6 cut(s) 256, 413, 422, 494, 550, 658
Lsp1109I GCAGC 9 cut(s) 349, 382, 449, 451, 454, 472, 494, 530, 541
LweI GCATC 2 cut(s) 532, 1207
MaeI CTAG 2 cut(s) 344, 476
MaeII ACGT 3 cut(s) 383, 526, 771
MaeIII GTNAC 4 cut(s) 702, 772, 876, 998
MalI GATC 5 cut(s) 224, 270, 831, 858, 1265
MbiI CCGCTC 1 cut(s) 842
MboI GATC 5 cut(s) 222, 268, 829, 856, 1263
MboII GAAGA 9 cut(s) 35, 98, 100, 315, 584, 611, 653, 1000, 1087
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 2 cut(s) 427, 655
MluCI AATT 2 cut(s) 1054, 1291
MmeI TCCRAC 3 cut(s) 400, 472, 572
MnlI CCTC 9 cut(s) 160, 168, 875, 880, 901, 1102, 1182, 1248, 1296
MroXI GAANNNNTTC 3 cut(s) 575, 588, 1079
MseI TTAA 8 cut(s) 261, 609, 636, 722, 741, 930, 1323, 1335
MslI CAYNNNNRTG 1 cut(s) 1007
MspA1I CMGCKG 4 cut(s) 485, 500, 521, 1103
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 7 cut(s) 135, 168, 453, 458, 665, 734, 755
MspR9I CCNGG 1 cut(s) 168
MvnI CGCG 3 cut(s) 371, 500, 1085
NciI CCSGG 1 cut(s) 168
NdeII GATC 5 cut(s) 222, 268, 829, 856, 1263
NheI GCTAGC 1 cut(s) 475
NlaIII CATG 2 cut(s) 797, 1012
NlaIV GGNNCC 2 cut(s) 496, 654
NmeAIII GCCGAG 1 cut(s) 840
NmuCI GTSAC 1 cut(s) 876
NruI TCGCGA 1 cut(s) 1085
PceI AGGCCT 1 cut(s) 1147
PciSI GCTCTTC 1 cut(s) 594
PdmI GAANNNNTTC 3 cut(s) 575, 588, 1079
PfeI GAWTC 7 cut(s) 91, 138, 187, 241, 309, 1126, 1151
PinAI ACCGGT 1 cut(s) 452
PsiI TTATAA 2 cut(s) 1172, 1247
Psp124BI GAGCTC 1 cut(s) 427
PspN4I GGNNCC 2 cut(s) 496, 654
PspPI GGNCC 1 cut(s) 455
PstI CTGCAG 1 cut(s) 787
PstNI CAGNNNCTG 2 cut(s) 254, 392
PsuI RGATCY 1 cut(s) 222
PvuII CAGCTG 2 cut(s) 485, 521
RruI TCGCGA 1 cut(s) 1085
RsaI GTAC 1 cut(s) 78
RsaNI GTAC 1 cut(s) 77
RseI CAYNNNNRTG 1 cut(s) 1007
Rsr2I CGGWCCG 1 cut(s) 455
RsrII CGGWCCG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 427
SacII CCGCGG 1 cut(s) 501
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 8 cut(s) 261, 609, 636, 722, 741, 930, 1323, 1335
Sau3AI GATC 5 cut(s) 222, 268, 829, 856, 1263
Sau96I GGNCC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 2 cut(s) 427, 655
SfaNI GCATC 2 cut(s) 532, 1207
SfcI CTRYAG 3 cut(s) 783, 966, 1098
Sfr303I CCGCGG 1 cut(s) 501
SgrBI CCGCGG 1 cut(s) 501
SinI GGWCC 1 cut(s) 455
SmiMI CAYNNNNRTG 1 cut(s) 1007
SmlI CTYRAG 2 cut(s) 159, 635
SmoI CTYRAG 2 cut(s) 159, 635
Sse9I AATT 2 cut(s) 1054, 1291
SseBI AGGCCT 1 cut(s) 1147
SspMI CTAG 2 cut(s) 344, 476
SstI GAGCTC 1 cut(s) 427
StuI AGGCCT 1 cut(s) 1147
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 5 cut(s) 622, 1072, 1118, 1178, 1307
TaiI ACGT 3 cut(s) 386, 529, 774
TaqI TCGA 5 cut(s) 69, 683, 710, 1154, 1187
TasI AATT 2 cut(s) 1054, 1291
TatI WGTACW 1 cut(s) 76
TauI GCSGC 4 cut(s) 174, 482, 500, 1217
TfiI GAWTC 7 cut(s) 91, 138, 187, 241, 309, 1126, 1151
Tru1I TTAA 8 cut(s) 261, 609, 636, 722, 741, 930, 1323, 1335
Tru9I TTAA 8 cut(s) 261, 609, 636, 722, 741, 930, 1323, 1335
TscAI CASTG 2 cut(s) 625, 1257
TseFI GTSAC 1 cut(s) 876
TseI GCWGC 9 cut(s) 362, 395, 437, 464, 467, 482, 485, 518, 554
Tsp45I GTSAC 1 cut(s) 876
TspDTI ATGAA 2 cut(s) 597, 782
TspRI CASTG 2 cut(s) 625, 1257
Vha464I CTTAAG 1 cut(s) 635
VpaK11BI GGWCC 1 cut(s) 455
XmnI GAANNNNTTC 3 cut(s) 575, 588, 1079
XspI CTAG 2 cut(s) 344, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.