Rorug04G0325800

SNF1-related protein kinase regulatory subunit gamma-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001126
Physical Location & Seq
Reverse (-)
50069457 .. 50071733
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0325800.1

Sequence Viewer

Length: 840 bp
ATGGTTGTTGCAGAGAAATCGAACGAAGCTGAGATCACCGAGCAGGACGCGCTTCTTCAGGCAATGTATCATCCACTTCCGATGATGCAGTATAAGAGAGTTGTAGATCTTCATAACTTGCTCGGAGGAGACTTTAATCAGACTAGTCTGGGTGGGATCAGTGACGAGAATGAACGAGACCGCTGGGGAAAGAAGCTTATGAACACTGACGCAAGCAGAGCTAAGAAGGAGTTGAAGCGCCTGAGGGTTTTGAGGATGGCAGTGATTTACATGATGATGTGTGTGCTGAATCTGTACGAGTACACGAAGAAGAACTTGGGGCCTCTGCGATCGACTGTGTGGACTGTGGAGGTTGCTATGATCACTATTGTTGGTCCAGTCTACCAGAGATTCAAGGGAGTGCCTGATGATTTCTTTGCTTTACTCGACACGAAGATTGATGAAGCAGCTGACAAGTTTGATAAGCATGCTCCTCCTTTGGCTAAGCAGGTGGTGTACCTAACTCTAGGTTCTATCCAGAAAGCATTGAAAAAGACCCAAGCATTTGTGAACGAAGCTCAGGCTGGCGGTCTACACACAGCTATTCATTCTGCAGCTACAGAATATAAGCTGCTTGTCTTCAACCAATCCATGAAGTTTTGGGTTGGACTCGAGTACCAGTGTCCTCCCATACACACAGTGGCAGAGAAGGCTACACCAGCTGCTGCTTACTTGTCCCGGAAATACAACAAGGTGGTAAGAGACTTGAACCTGAAGGGCTACACTATCTTTGGATATTTACCTTTGGTTCCGATCGATCAGATATCAACAGCACTTAATAGGGCGAGGCAGAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

279

Amino Acids

31.66

Weight (kDa)

9.42

Isoelectric Point (pI)

28.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 74 - 273 6.1e-63 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 478
Acc36I ACCTGC 1 cut(s) 478
AccI GTMKAC 2 cut(s) 381, 571
AccII CGCG 1 cut(s) 50
AciI CCGC 2 cut(s) 181, 567
AclWI GGATC 1 cut(s) 164
AcuI CTGAAG 2 cut(s) 41, 773
AdeI CACNNNGTG 1 cut(s) 679
AfaI GTAC 4 cut(s) 296, 302, 497, 656
AgsI TTSAA 5 cut(s) 235, 394, 529, 622, 748
AhlI ACTAGT 1 cut(s) 143
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 9 cut(s) 29, 196, 221, 449, 557, 581, 596, 610, 701
AluI AGCT 9 cut(s) 29, 196, 221, 449, 557, 581, 596, 610, 701
Alw26I GTCTC 3 cut(s) 123, 171, 735
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 1 cut(s) 320
ApeKI GCWGC 5 cut(s) 446, 593, 610, 701, 704
AspLEI GCGC 2 cut(s) 52, 240
AspS9I GGNCC 2 cut(s) 320, 374
AsuC2I CCSGG 1 cut(s) 718
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 1 cut(s) 374
AxyI CCTNAGG 1 cut(s) 242
BaeI ACNNNNGTAYC 2 cut(s) 638, 671
BbsI GAAGAC 1 cut(s) 610
BbvI GCAGC 5 cut(s) 458, 597, 605, 688, 691
BccI CCATC 1 cut(s) 250
BcgI CGANNNNNNTGC 1 cut(s) 34
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 1 cut(s) 718
BcoDI GTCTC 3 cut(s) 123, 171, 735
BcuI ACTAGT 1 cut(s) 143
BfaI CTAG 2 cut(s) 144, 506
BfmI CTRYAG 2 cut(s) 591, 597
BfoI RGCGCY 1 cut(s) 241
BfuAI ACCTGC 1 cut(s) 478
BglII AGATCT 1 cut(s) 106
BisI GCNGC 5 cut(s) 447, 594, 611, 702, 705
BlpI GCTNAGC 1 cut(s) 483
BlsI GCNGC 5 cut(s) 448, 595, 612, 703, 706
Bme1390I CCNGG 1 cut(s) 718
Bme18I GGWCC 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 2 cut(s) 320, 374
BmiI GGNNCC 2 cut(s) 321, 789
BmrFI CCNGG 1 cut(s) 718
BmsI GCATC 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 610
Bpu10I CCTNAGC 1 cut(s) 558
Bpu1102I GCTNAGC 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 718
Bsa29I ATCGAT 1 cut(s) 795
BsaI GGTCTC 1 cut(s) 171
Bse1I ACTGG 2 cut(s) 377, 658
Bse21I CCTNAGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 69
BseCI ATCGAT 1 cut(s) 795
BseGI GGATG 2 cut(s) 70, 261
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 21, 233, 572
BseNI ACTGG 2 cut(s) 377, 658
BseRI GAGGAG 2 cut(s) 141, 462
BseXI GCAGC 5 cut(s) 458, 597, 605, 688, 691
BseYI CCCAGC 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 50
Bsh1285I CGRYCG 2 cut(s) 332, 795
BshFI GGCC 1 cut(s) 322
BshVI ATCGAT 1 cut(s) 795
BsiEI CGRYCG 2 cut(s) 332, 795
BsiHKCI CYCGRG 1 cut(s) 650
BsiSI CCGG 1 cut(s) 718
BslFI GGGAC 1 cut(s) 700
BsmAI GTCTC 3 cut(s) 123, 171, 735
BsmFI GGGAC 1 cut(s) 700
BsnI GGCC 1 cut(s) 322
Bso31I GGTCTC 1 cut(s) 171
BsoBI CYCGRG 1 cut(s) 650
Bsp143I GATC 7 cut(s) 33, 106, 156, 329, 360, 792, 796
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 2 cut(s) 181, 567
BspANI GGCC 1 cut(s) 322
BspCNI CTCAG 3 cut(s) 22, 234, 571
BspDI ATCGAT 1 cut(s) 795
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 321, 789
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 69
BsrI ACTGG 2 cut(s) 377, 658
BssMI GATC 7 cut(s) 33, 106, 156, 329, 360, 792, 796
Bst4CI ACNGT 3 cut(s) 337, 346, 679
BstC8I GCNNGC 3 cut(s) 214, 468, 565
BstDEI CTNAG 5 cut(s) 30, 222, 242, 483, 558
BstF5I GGATG 2 cut(s) 70, 261
BstFNI CGCG 1 cut(s) 50
BstH2I RGCGCY 1 cut(s) 241
BstHHI GCGC 2 cut(s) 52, 240
BstKTI GATC 7 cut(s) 36, 109, 159, 332, 363, 795, 799
BstMAI GTCTC 3 cut(s) 123, 171, 735
BstMBI GATC 7 cut(s) 33, 106, 156, 329, 360, 792, 796
BstMCI CGRYCG 2 cut(s) 332, 795
BstMWI GCNNNNNNNGC 4 cut(s) 49, 218, 689, 698
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 716
BstSFI CTRYAG 2 cut(s) 591, 597
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 5 cut(s) 458, 597, 605, 688, 691
BstV2I GAAGAC 1 cut(s) 610
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
Bsu15I ATCGAT 1 cut(s) 795
Bsu36I CCTNAGG 1 cut(s) 242
BsuRI GGCC 1 cut(s) 322
BsuTUI ATCGAT 1 cut(s) 795
BtsCI GGATG 2 cut(s) 70, 261
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 5 cut(s) 166, 204, 267, 665, 684
BveI ACCTGC 1 cut(s) 478
Cac8I GCNNGC 3 cut(s) 214, 468, 565
CaiI CAGNNNCTG 1 cut(s) 704
CfoI GCGC 2 cut(s) 52, 240
Cfr13I GGNCC 2 cut(s) 320, 374
ClaI ATCGAT 1 cut(s) 795
CseI GACGC 2 cut(s) 56, 218
Csp6I GTAC 4 cut(s) 295, 301, 496, 655
CviAII CATG 3 cut(s) 271, 467, 631
CviQI GTAC 4 cut(s) 295, 301, 496, 655
DdeI CTNAG 5 cut(s) 30, 222, 242, 483, 558
DpnI GATC 7 cut(s) 35, 108, 158, 331, 362, 794, 798
DpnII GATC 7 cut(s) 33, 106, 156, 329, 360, 792, 796
DraIII CACNNNGTG 1 cut(s) 679
Eco31I GGTCTC 1 cut(s) 171
Eco32I GATATC 1 cut(s) 804
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 2 cut(s) 41, 773
Eco81I CCTNAGG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 320
EcoRV GATATC 1 cut(s) 804
FaeI CATG 3 cut(s) 274, 470, 634
FaiI YATR 9 cut(s) 93, 114, 200, 272, 359, 468, 606, 632, 671
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 1 cut(s) 700
FatI CATG 3 cut(s) 270, 466, 630
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 2 cut(s) 381, 571
Fnu4HI GCNGC 5 cut(s) 447, 594, 611, 702, 705
FokI GGATG 2 cut(s) 57, 268
Fsp4HI GCNGC 5 cut(s) 447, 594, 611, 702, 705
FspBI CTAG 2 cut(s) 144, 506
GlaI GCGC 2 cut(s) 51, 239
GluI GCNGC 5 cut(s) 447, 594, 611, 702, 705
GsaI CCCAGC 1 cut(s) 187
HaeII RGCGCY 1 cut(s) 241
HaeIII GGCC 1 cut(s) 322
HapII CCGG 1 cut(s) 718
HgaI GACGC 2 cut(s) 56, 218
HhaI GCGC 2 cut(s) 52, 240
Hin1II CATG 3 cut(s) 274, 470, 634
Hin6I GCGC 2 cut(s) 50, 238
HinP1I GCGC 2 cut(s) 50, 238
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 3 cut(s) 289, 390, 648
HpaII CCGG 1 cut(s) 718
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 6 cut(s) 303, 342, 382, 496, 550, 572
Hpy188I TCNGA 5 cut(s) 81, 125, 141, 792, 801
Hpy188III TCNNGA 1 cut(s) 517
Hpy8I GTNNAC 6 cut(s) 303, 342, 382, 496, 550, 572
HpyAV CCTTC 3 cut(s) 220, 682, 748
HpyCH4III ACNGT 3 cut(s) 337, 346, 679
HpyCH4V TGCA 3 cut(s) 11, 88, 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 218, 689, 698
HpyF3I CTNAG 5 cut(s) 30, 222, 242, 483, 558
Hsp92II CATG 3 cut(s) 274, 470, 634
HspAI GCGC 2 cut(s) 50, 238
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 7 cut(s) 33, 106, 156, 329, 360, 792, 796
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 5 cut(s) 458, 597, 605, 688, 691
LweI GCATC 1 cut(s) 75
MaeI CTAG 2 cut(s) 144, 506
MaeIII GTNAC 1 cut(s) 161
MalI GATC 7 cut(s) 35, 108, 158, 331, 362, 794, 798
MboI GATC 7 cut(s) 33, 106, 156, 329, 360, 792, 796
MboII GAAGA 6 cut(s) 47, 101, 319, 322, 445, 610
MflI RGATCY 1 cut(s) 106
MlyI GAGTC 1 cut(s) 642
MmeI TCCRAC 1 cut(s) 625
MnlI CCTC 8 cut(s) 119, 237, 246, 333, 343, 483, 675, 819
MseI TTAA 2 cut(s) 135, 816
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 3 cut(s) 183, 449, 701
MspI CCGG 1 cut(s) 718
MspR9I CCNGG 1 cut(s) 718
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 4 cut(s) 49, 218, 689, 698
NciI CCSGG 1 cut(s) 718
NdeII GATC 7 cut(s) 33, 106, 156, 329, 360, 792, 796
NlaIII CATG 3 cut(s) 274, 470, 634
NlaIV GGNNCC 2 cut(s) 321, 789
NmuCI GTSAC 1 cut(s) 161
NspI RCATGY 1 cut(s) 470
PaeI GCATGC 1 cut(s) 470
PaeR7I CTCGAG 1 cut(s) 650
PaqCI CACCTGC 1 cut(s) 478
PfeI GAWTC 2 cut(s) 289, 390
PfoI TCCNGGA 1 cut(s) 716
PkrI GCNGC 5 cut(s) 448, 595, 612, 703, 706
Ple19I CGATCG 2 cut(s) 332, 795
PleI GAGTC 1 cut(s) 642
PpsI GAGTC 1 cut(s) 642
PspFI CCCAGC 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 321, 789
PspPI GGNCC 2 cut(s) 320, 374
PspXI VCTCGAGB 1 cut(s) 650
PstI CTGCAG 1 cut(s) 595
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 1 cut(s) 106
PvuI CGATCG 2 cut(s) 332, 795
PvuII CAGCTG 2 cut(s) 449, 701
RsaI GTAC 4 cut(s) 296, 302, 497, 656
RsaNI GTAC 4 cut(s) 295, 301, 496, 655
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 2 cut(s) 135, 816
SatI GCNGC 5 cut(s) 447, 594, 611, 702, 705
Sau3AI GATC 7 cut(s) 33, 106, 156, 329, 360, 792, 796
Sau96I GGNCC 2 cut(s) 320, 374
SchI GAGTC 1 cut(s) 642
ScrFI CCNGG 1 cut(s) 718
SfaNI GCATC 1 cut(s) 75
SfcI CTRYAG 2 cut(s) 591, 597
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 1 cut(s) 374
SlaI CTCGAG 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
SpeI ACTAGT 1 cut(s) 143
SphI GCATGC 1 cut(s) 470
SsiI CCGC 2 cut(s) 181, 567
SspMI CTAG 2 cut(s) 144, 506
StyD4I CCNGG 1 cut(s) 716
TaaI ACNGT 3 cut(s) 337, 346, 679
TaqI TCGA 5 cut(s) 20, 332, 426, 651, 795
TatI WGTACW 1 cut(s) 300
TfiI GAWTC 2 cut(s) 289, 390
Tru1I TTAA 2 cut(s) 135, 816
Tru9I TTAA 2 cut(s) 135, 816
TscAI CASTG 5 cut(s) 166, 211, 267, 665, 684
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 5 cut(s) 446, 593, 610, 701, 704
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 6 cut(s) 101, 186, 215, 456, 575, 647
TspRI CASTG 5 cut(s) 166, 211, 267, 665, 684
VpaK11BI GGWCC 1 cut(s) 374
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XhoI CTCGAG 1 cut(s) 650
XmiI GTMKAC 2 cut(s) 381, 571
XspI CTAG 2 cut(s) 144, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.