Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001126

Physical Location & Seq

Genomic Coordinates Forward (+)

59414230 .. 59416089

Gene Structure 1860 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G032260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGAAGCGCGAAATAGGCAGACTAGTCGCTGATGGCTTATACAGAGAAGCCCTATGCTTATATACTCAACGCCACTCCGCTTCTTTTCCCCCTCACAAATTCACATTCCCTCCGCTTCTCAAAGCCTGTGCCAAGCTCCGATCAGCCCCTCAAGCCCAAATGGTCCACACGCATCTCCTCAAAACCGGGTTCTTCTCCGACGTCTACTCAGCCACCGCTCTCACCGACGCTTACATGAAGCTCAATCTCATGGACGATGCGCTCAAGGTGTTCGACGAAATGCCTGACCGAAACTTGGCTTCGGTAAACGCTGTGATTTCCGGGCTGTTGCATAATGGACATCGCAGAGAGGCTTTAAAGGTGTTCAAGAGCGTTGGTTTTGGAGGGTTCGGGTTGAATTCGGTTACTATAGCCAGTGTGCTTTCGGCGTGTGACAATGCGAAGGAGGGGATGGGAATGCATTGCGCTGCGGTGAAGCTCGGGGTTGAGAGTGATGTTTATGTTGCTACATCAGCTGTGAGTATGTATTCGAGCTGTGGAGAGTTGGTTTCTGCTGCAAAGTTGTTTGAAGATATGCCTGTCAAGAATGTGGTGAGCTATAATGCTTTTATAACAGGGCTTATGCAGAATGGGGTTCCAAGGGTGGTGTTGGATGTGTTTAAGAAAATGAGAGCATGTAGAGGTGAAAATCCGAATTCAGTGACATTGGTTTCGGTTCTCTCTGCTTGTGCTAGTGTTTTGTATCTCCGATTTGGCAGGCAGGTTCATGGTTTGATCGTGAAAATTGCGAGTGGGATTGATGCTATGACTAGAACAGCGCTTGTCGACATGTATTCCAAGTGTGGGTGTTGGCAGCTCGGGTACCAAATTTTCAAAGATCTAGATGAAACAAGGACCTTGTTTATATGGAATGCCACGATTGCAGGAATGATGTTGAATGCGCAGTGGGAGATTGCTGTTGAGCTTTTCGAACAGCTAGAATCTGAAGGGTTTAAGCCTGATTCAGTGACTTGGAATTCAATGATCAGCGGGTTTTCACAACTAGGGATGGGAAACGAAGCTTCCAAGTATTTTAAGAGAATGCAATCGACTGGTGTAGCTCCGAGTTTGAAATCTGTCACTAGTCTTTTGCAAGCCTGCGCAGATTCATCTGCTCTGCAATGTGGACAAGAGGTTCATGGGCATGCAATTAGGACTGATATTAGCAGTGATCTGTTCTTTTCAACCGCTCTCATTGACATGTACATGAAATGTGGGCAGTCTTCTTGGGCAAGAGGAGTTTTTGATTCCTTTCATGTAAAACCTGACGATCCAGCATTTTGGAATGCAACAATATCTGGGTATGGAAGAGAAGGAGAGAATGAATCTGCATTTCGAATATTTGATCAGATGCTCGAAGAAAAAGTACAACCAAATGCAGCAACTTTTATAAGTCTTCTATCTATGTGTAGTCACAGTGGTTTAATTGACAAGGGATGGCAAATTTTTAGGATGATGGATAGAGATTTTGGAATGAAACCAGATCCGAAACATTATGGTTGTATGATTGACCTTTTAGGTAGATCAGGCAGGTTGGATGAAGCCCGGGAATTAACACACGAGTTATCAGAGCCTTCTGGATCAGTTTTTGCTTCTTTGCTAGGTGCTTGCGAGTCCCATTTAAATCTAGAACTGGGGAAAGAAATGGCCATAAAACTTTCAGAATTAGAGCCAGAGAACCCTACACCTTTCCTGATACTGTCCAAGATATATGCTGGGCTTGGGAGGTGGAAGGATGCAGAAATCATTAGGGGAATGATGGATGATCAAAAGTCAAGAAAGCTCCCTGGCTTTAGCTTATTACAATTGCATGAAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

619

Amino Acids

68.37

Weight (kDa)

6.48

Isoelectric Point (pI)

35.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	195 - 244	3.8e-08	PPR repeat family
PPR_2	PF13041	301 - 345	2.1e-09	PPR repeat family
PPR_long	PF17177	312 - 422	4.4e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	322 - 382	4e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	330 - 361	7.3e-06	PPR repeat
PPR_2	PF13041	333 - 381	8.7e-11	PPR repeat family
PPR	PF01535	336 - 365	3.3e-08	PPR repeat
PPR_2	PF13041	440 - 484	2.5e-10	PPR repeat family
E_motif	PF20431	553 - 614	2.3e-12	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001126)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02750
fragaria_vesca	FvH4_4g31750 FvH4_4g31750 FvH4_4g31750
malus_domestica	MD16G1077500.v1.1
prunus_persica	Prupe.1G269600_v2.0.a1
pyrus_communis	pycom16g06680
rosa_chinensis	RchiOBHm_Chr3g0480041 RchiOBHm_Chr4g0440351 RchiOBHm_Chr5g0020671 RchiOBHm_Chr5g0027821 RchiOBHm_Chr5g0045351 RchiOBHm_Chr5g0062441 RchiOBHm_Chr6g0278731
rosa_laevigata	RLG00000006189 RLG00000009789 RLG00000009916 RLG00000013392 RLG00000013526 RLG00000013972 RLG00000020257 RLG00000031746 RLG00000031753 RLG00000033045 RLG00000033161
rosa_multiflora	Rmu_co8052728.1_g000001 Rmu_sc0001159.1_g000033 Rmu_sc0001685.1_g000045 Rmu_sc0003684.1_g000002 Rmu_sc0004520.1_g000016 Rmu_sc0012764.1_g000009 Rmu_sc0017399.1_g000002
rosa_roxburghii	Rroxscaffold_5G00343960 Rroxscaffold_5G00381230
rosa_rugosa	Rorug02G0009200 Rorug04G0056800 Rorug04G0056900 Rorug04G0325800 Rorug04G0335700
rosa_samantha	Rh2BG469200 Rh3CG261100 Rh3DG257000 Rh4AG376800 Rh4BG090100 Rh4BG389800 Rh4CG404600 Rh4DG383500 Rh5AG303800 Rh5AG409600 Rh5BG076900 Rh5BG193800 Rh5BG193900 Rh5BG199300 Rh5CG163100 Rh5CG214100 Rh5DG322500 Rh7BG133700
rosa_wichuraiana	Rw4G032260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	611, 1430
AatII	GACGTC	1 cut(s)	204
Acc16I	TGCGCA	2 cut(s)	942, 1141
Acc36I	ACCTGC	2 cut(s)	751, 1560
Acc65I	GGTACC	1 cut(s)	861
AccB1I	GGYRCC	1 cut(s)	861
AccBSI	CCGCTC	2 cut(s)	218, 1229
AccI	GTMKAC	2 cut(s)	204, 825
AccII	CGCG	1 cut(s)	9
AciI	CCGC	6 cut(s)	78, 113, 216, 470, 1029, 1227
AclWI	GGATC	3 cut(s)	1304, 1517, 1627
AcoI	YGGCCR	1 cut(s)	1686
AcsI	RAATTY	6 cut(s)	98, 397, 694, 867, 1015, 1482
AcuI	CTGAAG	1 cut(s)	1005
AcyI	GRCGYC	1 cut(s)	201
AfaI	GTAC	3 cut(s)	863, 1244, 1407
AfeI	AGCGCT	1 cut(s)	819
AfiI	CCNNNNNNNGG	2 cut(s)	295, 843
AflIII	ACRYGT	2 cut(s)	828, 1239
AgsI	TTSAA	8 cut(s)	367, 397, 569, 874, 937, 1020, 1111, 1224
AhlI	ACTAGT	2 cut(s)	22, 1121
AjnI	CCWGG	1 cut(s)	1825
AloI	GAACNNNNNNTCC	2 cut(s)	1158, 1190
AlwI	GGATC	3 cut(s)	1304, 1517, 1627
Ama87I	CYCGRG	3 cut(s)	479, 857, 1584
Aor51HI	AGCGCT	1 cut(s)	819
AoxI	GGCC	1 cut(s)	1686
ApeKI	GCWGC	4 cut(s)	467, 554, 853, 1418
ApoI	RAATTY	6 cut(s)	98, 397, 694, 867, 1015, 1482
ArsI	GACNNNNNNTTYG	2 cut(s)	694, 726
Asp718I	GGTACC	1 cut(s)	861
AspLEI	GCGC	6 cut(s)	9, 262, 467, 820, 943, 1142
AspS9I	GGNCC	2 cut(s)	163, 894
AsuC2I	CCSGG	4 cut(s)	187, 322, 1585, 1586
AsuHPI	GGTGA	4 cut(s)	214, 484, 604, 695
AsuII	TTCGAA	2 cut(s)	969, 1375
AvaI	CYCGRG	3 cut(s)	479, 857, 1584
AvaII	GGWCC	2 cut(s)	163, 894
BalI	TGGCCA	1 cut(s)	1688
BanI	GGYRCC	1 cut(s)	861
BarI	GAAGNNNNNNTAC	2 cut(s)	1389, 1421
BauI	CACGAG	1 cut(s)	1598
BbsI	GAAGAC	2 cut(s)	1254, 1427
BbvI	GCAGC	4 cut(s)	454, 541, 865, 1430
BccI	CCATC	6 cut(s)	26, 445, 1042, 1470, 1489, 1792
BcgI	CGANNNNNNTGC	2 cut(s)	7, 41
BciT130I	CCWGG	1 cut(s)	1827
BclI	TGATCA	3 cut(s)	1023, 1384, 1804
BcnI	CCSGG	4 cut(s)	187, 322, 1585, 1586
BcuI	ACTAGT	2 cut(s)	22, 1121
BfaI	CTAG	9 cut(s)	23, 732, 810, 881, 977, 1043, 1122, 1640, 1667
BfmI	CTRYAG	1 cut(s)	408
BfoI	RGCGCY	1 cut(s)	821
BfuAI	ACCTGC	2 cut(s)	751, 1560
BglII	AGATCT	1 cut(s)	877
BisI	GCNGC	4 cut(s)	468, 555, 854, 1419
BlsI	GCNGC	4 cut(s)	469, 556, 855, 1420
Bme1390I	CCNGG	5 cut(s)	187, 322, 1585, 1586, 1827
Bme18I	GGWCC	2 cut(s)	163, 894
BmeT110I	CYCGRG	3 cut(s)	479, 857, 1584
BmgT120I	GGNCC	2 cut(s)	163, 894
BmiI	GGNNCC	2 cut(s)	636, 863
BmrFI	CCNGG	5 cut(s)	187, 322, 1585, 1586, 1827
BmrI	ACTGGG	1 cut(s)	1682
BmsI	GCATC	5 cut(s)	181, 247, 790, 1380, 1765
BmuI	ACTGGG	1 cut(s)	1682
BpiI	GAAGAC	2 cut(s)	1254, 1427
Bpu14I	TTCGAA	2 cut(s)	969, 1375
BpuEI	CTTGAG	2 cut(s)	135, 248
BpuMI	CCSGG	4 cut(s)	187, 322, 1585, 1586
BsaHI	GRCGYC	1 cut(s)	201
BsaJI	CCNNGG	3 cut(s)	638, 1584, 1825
BsaXI	ACNNNNNCTCC	4 cut(s)	94, 124, 531, 561
Bsc4I	CCNNNNNNNGG	2 cut(s)	295, 843
Bse1I	ACTGG	3 cut(s)	414, 1096, 1677
Bse3DI	GCAATG	2 cut(s)	460, 1166
BseBI	CCWGG	1 cut(s)	1827
BseDI	CCNNGG	3 cut(s)	638, 1584, 1825
BseGI	GGATG	8 cut(s)	456, 658, 1053, 1481, 1497, 1582, 1780, 1807
BseLI	CCNNNNNNNGG	2 cut(s)	295, 843
BseMI	GCAATG	2 cut(s)	460, 1166
BseMII	CTCAG	1 cut(s)	222
BseNI	ACTGG	3 cut(s)	414, 1096, 1677
BseRI	GAGGAG	2 cut(s)	167, 1290
BseXI	GCAGC	4 cut(s)	454, 541, 865, 1430
BseYI	CCCAGC	1 cut(s)	1754
Bsh1236I	CGCG	1 cut(s)	9
BshFI	GGCC	1 cut(s)	1688
BshNI	GGYRCC	1 cut(s)	861
BsiHKCI	CYCGRG	3 cut(s)	479, 857, 1584
BsiSI	CCGG	3 cut(s)	186, 321, 1585
BslFI	GGGAC	1 cut(s)	1639
BslI	CCNNNNNNNGG	2 cut(s)	295, 843
BsmFI	GGGAC	1 cut(s)	1639
BsmI	GAATGC	5 cut(s)	462, 916, 943, 1086, 1330
BsnI	GGCC	1 cut(s)	1688
BsoBI	CYCGRG	3 cut(s)	479, 857, 1584
Bsp119I	TTCGAA	2 cut(s)	969, 1375
Bsp1407I	TGTACA	1 cut(s)	1242
BspACI	CCGC	6 cut(s)	78, 113, 216, 470, 1029, 1227
BspANI	GGCC	1 cut(s)	1688
BspCNI	CTCAG	1 cut(s)	221
BspFNI	CGCG	1 cut(s)	9
BspLI	GGNNCC	2 cut(s)	636, 863
BspMI	ACCTGC	2 cut(s)	751, 1560
BspPI	GGATC	3 cut(s)	1304, 1517, 1627
BspT104I	TTCGAA	2 cut(s)	969, 1375
BspT107I	GGYRCC	1 cut(s)	861
BsrBI	CCGCTC	2 cut(s)	218, 1229
BsrDI	GCAATG	2 cut(s)	460, 1166
BsrGI	TGTACA	1 cut(s)	1242
BsrI	ACTGG	3 cut(s)	414, 1096, 1677
BssECI	CCNNGG	3 cut(s)	638, 1584, 1825
BssNI	GRCGYC	1 cut(s)	201
BssSI	CACGAG	1 cut(s)	1598
BssT1I	CCWWGG	1 cut(s)	638
Bst2BI	CACGAG	1 cut(s)	1598
Bst2UI	CCWGG	1 cut(s)	1827
Bst4CI	ACNGT	2 cut(s)	1457, 1740
Bst6I	CTCTTC	1 cut(s)	1342
BstACI	GRCGYC	1 cut(s)	201
BstAUI	TGTACA	1 cut(s)	1242
BstBI	TTCGAA	2 cut(s)	969, 1375
BstC8I	GCNNGC	5 cut(s)	758, 1134, 1138, 1185, 1648
BstDEI	CTNAG	1 cut(s)	208
BstF5I	GGATG	8 cut(s)	456, 658, 1053, 1481, 1497, 1582, 1780, 1807
BstFNI	CGCG	1 cut(s)	9
BstH2I	RGCGCY	1 cut(s)	821
BstHHI	GCGC	6 cut(s)	9, 262, 467, 820, 943, 1142
BstMWI	GCNNNNNNNGC	4 cut(s)	15, 152, 512, 920
BstNI	CCWGG	1 cut(s)	1827
BstNSI	RCATGY	4 cut(s)	678, 832, 1187, 1243
BstSCI	CCNGG	5 cut(s)	185, 320, 1583, 1584, 1825
BstSFI	CTRYAG	1 cut(s)	408
BstUI	CGCG	1 cut(s)	9
BstV1I	GCAGC	4 cut(s)	454, 541, 865, 1430
BstV2I	GAAGAC	2 cut(s)	1254, 1427
BstX2I	RGATCY	2 cut(s)	877, 1522
BstXI	CCANNNNNNTGG	1 cut(s)	1320
BstYI	RGATCY	2 cut(s)	877, 1522
BsuRI	GGCC	1 cut(s)	1688
BtgZI	GCGATG	1 cut(s)	326
BtsCI	GGATG	8 cut(s)	456, 658, 1053, 1481, 1497, 1582, 1780, 1807
BtsI	GCAGTG	2 cut(s)	950, 1213
BtsIMutI	CAGTG	6 cut(s)	421, 705, 950, 1011, 1213, 1462
BveI	ACCTGC	2 cut(s)	751, 1560
Cac8I	GCNNGC	5 cut(s)	758, 1134, 1138, 1185, 1648
CfoI	GCGC	6 cut(s)	9, 262, 467, 820, 943, 1142
Cfr13I	GGNCC	2 cut(s)	163, 894
Cfr9I	CCCGGG	1 cut(s)	1584
CseI	GACGC	1 cut(s)	236
Csp6I	GTAC	3 cut(s)	862, 1243, 1406
CviQI	GTAC	3 cut(s)	862, 1243, 1406
DdeI	CTNAG	1 cut(s)	208
DraI	TTTAAA	2 cut(s)	357, 1662
EaeI	YGGCCR	1 cut(s)	1686
Eam1104I	CTCTTC	1 cut(s)	1342
EarI	CTCTTC	1 cut(s)	1342
Eco130I	CCWWGG	1 cut(s)	638
Eco47I	GGWCC	2 cut(s)	163, 894
Eco47III	AGCGCT	1 cut(s)	819
Eco57I	CTGAAG	1 cut(s)	1005
Eco88I	CYCGRG	3 cut(s)	479, 857, 1584
EcoO109I	RGGNCCY	1 cut(s)	894
EcoRI	GAATTC	3 cut(s)	397, 694, 1015
EcoRII	CCWGG	1 cut(s)	1825
EcoT14I	CCWWGG	1 cut(s)	638
EcoT22I	ATGCAT	1 cut(s)	462
ErhI	CCWWGG	1 cut(s)	638
FaqI	GGGAC	1 cut(s)	1639
FauI	CCCGC	1 cut(s)	1022
FbaI	TGATCA	3 cut(s)	1023, 1384, 1804
FblI	GTMKAC	2 cut(s)	204, 825
Fnu4HI	GCNGC	4 cut(s)	468, 555, 854, 1419
FokI	GGATG	8 cut(s)	463, 665, 1060, 1488, 1504, 1589, 1787, 1814
Fsp4HI	GCNGC	4 cut(s)	468, 555, 854, 1419
FspBI	CTAG	9 cut(s)	23, 732, 810, 881, 977, 1043, 1122, 1640, 1667
FspI	TGCGCA	2 cut(s)	942, 1141
GlaI	GCGC	6 cut(s)	8, 261, 466, 819, 942, 1141
GluI	GCNGC	4 cut(s)	468, 555, 854, 1419
GsaI	CCCAGC	1 cut(s)	1758
HaeII	RGCGCY	1 cut(s)	821
HaeIII	GGCC	1 cut(s)	1688
HapII	CCGG	3 cut(s)	186, 321, 1585
HgaI	GACGC	1 cut(s)	236
HhaI	GCGC	6 cut(s)	9, 262, 467, 820, 943, 1142
Hin1I	GRCGYC	1 cut(s)	201
Hin6I	GCGC	6 cut(s)	7, 260, 465, 818, 941, 1140
HinP1I	GCGC	6 cut(s)	7, 260, 465, 818, 941, 1140
HincII	GTYRAC	1 cut(s)	826
HindII	GTYRAC	1 cut(s)	826
HindIII	AAGCTT	1 cut(s)	1059
HinfI	GANTC	6 cut(s)	980, 1001, 1145, 1286, 1364, 1652
HpaII	CCGG	3 cut(s)	186, 321, 1585
HphI	GGTGA	4 cut(s)	214, 484, 604, 695
Hpy166II	GTNNAC	5 cut(s)	166, 205, 307, 826, 1166
Hpy188III	TCNNGA	8 cut(s)	367, 583, 778, 881, 1617, 1667, 1732, 1815
Hpy8I	GTNNAC	5 cut(s)	166, 205, 307, 826, 1166
Hpy99I	CGWCG	3 cut(s)	203, 230, 278
HpyAV	CCTTC	5 cut(s)	436, 980, 1346, 1623, 1765
HpyCH4III	ACNGT	2 cut(s)	1457, 1740
HpyCH4IV	ACGT	1 cut(s)	201
HpyF10VI	GCNNNNNNNGC	4 cut(s)	15, 152, 512, 920
HpyF3I	CTNAG	1 cut(s)	208
HpySE526I	ACGT	1 cut(s)	201
Hsp92I	GRCGYC	1 cut(s)	201
HspAI	GCGC	6 cut(s)	7, 260, 465, 818, 941, 1140
KpnI	GGTACC	1 cut(s)	865
Ksp22I	TGATCA	3 cut(s)	1023, 1384, 1804
LmnI	GCTCC	3 cut(s)	141, 1105, 1827
Lsp1109I	GCAGC	4 cut(s)	454, 541, 865, 1430
LweI	GCATC	5 cut(s)	181, 247, 790, 1380, 1765
MaeI	CTAG	9 cut(s)	23, 732, 810, 881, 977, 1043, 1122, 1640, 1667
MaeII	ACGT	1 cut(s)	201
MaeIII	GTNAC	6 cut(s)	403, 431, 700, 1006, 1117, 1451
MbiI	CCGCTC	2 cut(s)	218, 1229
MboII	GAAGA	6 cut(s)	184, 581, 1254, 1359, 1409, 1427
MfeI	CAATTG	1 cut(s)	1844
MflI	RGATCY	2 cut(s)	877, 1522
MlsI	TGGCCA	1 cut(s)	1688
MluNI	TGGCCA	1 cut(s)	1688
MlyI	GAGTC	1 cut(s)	1661
MmeI	TCCRAC	3 cut(s)	222, 630, 1554
Mox20I	TGGCCA	1 cut(s)	1688
Mph1103I	ATGCAT	1 cut(s)	462
MscI	TGGCCA	1 cut(s)	1688
MseI	TTAA	7 cut(s)	356, 660, 993, 1074, 1463, 1592, 1661
MslI	CAYNNNNRTG	3 cut(s)	1182, 1238, 1244
Msp20I	TGGCCA	1 cut(s)	1688
MspA1I	CMGCKG	2 cut(s)	515, 1029
MspI	CCGG	3 cut(s)	186, 321, 1585
MspR9I	CCNGG	5 cut(s)	187, 322, 1585, 1586, 1827
MunI	CAATTG	1 cut(s)	1844
Mva1269I	GAATGC	5 cut(s)	462, 916, 943, 1086, 1330
MvaI	CCWGG	1 cut(s)	1827
MvnI	CGCG	1 cut(s)	9
MwoI	GCNNNNNNNGC	4 cut(s)	15, 152, 512, 920
NciI	CCSGG	4 cut(s)	187, 322, 1585, 1586
NlaIV	GGNNCC	2 cut(s)	636, 863
NmuCI	GTSAC	5 cut(s)	431, 700, 1006, 1117, 1451
NsbI	TGCGCA	2 cut(s)	942, 1141
NsiI	ATGCAT	1 cut(s)	462
NspI	RCATGY	4 cut(s)	678, 832, 1187, 1243
NspV	TTCGAA	2 cut(s)	969, 1375
PaeI	GCATGC	1 cut(s)	1187
PciI	ACATGT	2 cut(s)	828, 1239
PctI	GAATGC	5 cut(s)	462, 916, 943, 1086, 1330
PfeI	GAWTC	5 cut(s)	980, 1001, 1145, 1286, 1364
PkrI	GCNGC	4 cut(s)	469, 556, 855, 1420
PleI	GAGTC	1 cut(s)	1660
PpsI	GAGTC	1 cut(s)	1660
PpuMI	RGGWCCY	1 cut(s)	894
PscI	ACATGT	2 cut(s)	828, 1239
PsiI	TTATAA	2 cut(s)	611, 1430
Psp5II	RGGWCCY	1 cut(s)	894
Psp6I	CCWGG	1 cut(s)	1825
PspFI	CCCAGC	1 cut(s)	1754
PspGI	CCWGG	1 cut(s)	1825
PspN4I	GGNNCC	2 cut(s)	636, 863
PspPI	GGNCC	2 cut(s)	163, 894
PspPPI	RGGWCCY	1 cut(s)	894
PsuI	RGATCY	2 cut(s)	877, 1522
PvuII	CAGCTG	1 cut(s)	515
RsaI	GTAC	3 cut(s)	863, 1244, 1407
RsaNI	GTAC	3 cut(s)	862, 1243, 1406
RseI	CAYNNNNRTG	3 cut(s)	1182, 1238, 1244
SalI	GTCGAC	1 cut(s)	824
SaqAI	TTAA	7 cut(s)	356, 660, 993, 1074, 1463, 1592, 1661
SatI	GCNGC	4 cut(s)	468, 555, 854, 1419
Sau96I	GGNCC	2 cut(s)	163, 894
SchI	GAGTC	1 cut(s)	1661
ScrFI	CCNGG	5 cut(s)	187, 322, 1585, 1586, 1827
SfaNI	GCATC	5 cut(s)	181, 247, 790, 1380, 1765
SfcI	CTRYAG	1 cut(s)	408
SfuI	TTCGAA	2 cut(s)	969, 1375
SinI	GGWCC	2 cut(s)	163, 894
SmaI	CCCGGG	1 cut(s)	1586
SmiI	ATTTAAAT	1 cut(s)	1662
SmiMI	CAYNNNNRTG	3 cut(s)	1182, 1238, 1244
SmlI	CTYRAG	2 cut(s)	150, 263
SmoI	CTYRAG	2 cut(s)	150, 263
SpeI	ACTAGT	2 cut(s)	22, 1121
SphI	GCATGC	1 cut(s)	1187
SsiI	CCGC	6 cut(s)	78, 113, 216, 470, 1029, 1227
SspI	AATATT	1 cut(s)	1380
SspMI	CTAG	9 cut(s)	23, 732, 810, 881, 977, 1043, 1122, 1640, 1667
StyD4I	CCNGG	5 cut(s)	185, 320, 1583, 1584, 1825
StyI	CCWWGG	1 cut(s)	638
SwaI	ATTTAAAT	1 cut(s)	1662
TaaI	ACNGT	2 cut(s)	1457, 1740
TaiI	ACGT	1 cut(s)	204
TaqI	TCGA	7 cut(s)	273, 530, 825, 969, 1088, 1375, 1395
TaqII	GACCGA	1 cut(s)	303
TatI	WGTACW	2 cut(s)	1242, 1405
TfiI	GAWTC	5 cut(s)	980, 1001, 1145, 1286, 1364
Tru1I	TTAA	7 cut(s)	356, 660, 993, 1074, 1463, 1592, 1661
Tru9I	TTAA	7 cut(s)	356, 660, 993, 1074, 1463, 1592, 1661
TscAI	CASTG	6 cut(s)	421, 705, 950, 1011, 1213, 1462
TseFI	GTSAC	5 cut(s)	431, 700, 1006, 1117, 1451
TseI	GCWGC	4 cut(s)	467, 554, 853, 1418
Tsp45I	GTSAC	5 cut(s)	431, 700, 1006, 1117, 1451
TspMI	CCCGGG	1 cut(s)	1584
TspRI	CASTG	6 cut(s)	421, 705, 950, 1011, 1213, 1462
VpaK11BI	GGWCC	2 cut(s)	163, 894
XapI	RAATTY	6 cut(s)	98, 397, 694, 867, 1015, 1482
XbaI	TCTAGA	2 cut(s)	880, 1666
XceI	RCATGY	4 cut(s)	678, 832, 1187, 1243
XmaI	CCCGGG	1 cut(s)	1584
XmiI	GTMKAC	2 cut(s)	204, 825
XspI	CTAG	9 cut(s)	23, 732, 810, 881, 977, 1043, 1122, 1640, 1667
ZraI	GACGTC	1 cut(s)	202
Zsp2I	ATGCAT	1 cut(s)	462

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.