Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0003883

Physical Location & Seq

Genomic Coordinates Reverse (-)

60083692 .. 60086137

Gene Structure 2446 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG383700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGACAGAAGCAGCACAAGAAGGAAGGGGAATTCCCAAGAGTCCTAAGAGGTCAAGCTGCTATGATGCCTACTTCGAAAGGATTCAGTTCAGGAAGAAATTACCGCATGAGCTTCAAGAGAAATTAACGTATGCATTTTCAAAAGTTCCAGTTTCATCTTTCCAATCCCTTCAGGGAGGAAAAGTAATTGAGATTGTAGCAGATATGTCTATTCCTGATGCAGTCAAGACTCTCTCAGAGCACAAGATTTTGTCAGCTCCGGTGATTACTGTGCCGGATCAAACCTATTCGGATTGGAGAGGAAGGTACCTTGGAATCATAGATTACTCTGCCGTTCTTGTTTGGGTGTTGGAGAGTGCAGAACATGCTGCTGAAGGTGCCTCGTCAGCTAGTTATGCAGCAACCACGGCTGGTGGAATTGGTGGTGCAGGAGCTGTTGGCGCCATTGGAGCTGTAGCATTGGGGGTCACAGGTCCTGCTGCAATTGCAGGCCTAACTGCTGCTGCAGTTTGTTCCGCTGTAGCCGGGAGTGCTGTTGGAAAAATAGATATAGCTGCAGAGAATGAGGACAAAGATTCTCCCACAACGGGTGATAAATTGACCAAAGATCTTGACAAGGTCATACTGCAAGATGAACCCTGCAAGTCAACAACAGTAAAGTCAATAGTTAAATCCTTCGGGGAAACACCTTTCCTTCCGGTAGCAGAAGATAGTTCTATGTTGACCATCTTGCTACTACTCTCAAAATATAGGATGAGGAATGTGCCTGTAATAGAAAATGGCCAACCAGGACTTAAGAACTACATTACTCAATCAGCAGTTATACGCGGTCTTGAAGGATGCAAAGGAATGGAGTGGTTTGACTGCATTGCAGAAAAACCTATCTCGGATCTGGGACTTCCCTTTATGTCCTCTGATGAGGTTATTAGTATCCAGAGTGATGATCTGATACTGAAAGCTTATAAGAAAATGAAAGAAAAAAAAATGGGTGGTCTTCCAGTTGTAGAGGGACAAACGAAGAAGCTAGTTGGTAATGTCAGCGTAAGCGATATCAGATACATGTTTCTCAAACACGATCTCCTCTCCAATTTCAGGAAGCTCACTGCTATGGAATTCCTAACCACCATCTCAACAAGCAAAGAACCTGGAAAGCCCATCCCACCAATTACATGCAATATCGAGTCGACTCTTGGTAATGTGATTGAGATTCTTGCTTCCAAGTCCGTTCATAGGATCTACGCGGTAAGTGCGGAAGAAGGAGAAGTTCTTGGTGTGATTACACTCAGAGACGTCATTTCTTGCTTCATATATGAACCCCCCAATCACTTTGATGCCTACTTGGGGTTTGCAGTGAAGGAGATACTGAAGCAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS

Protein Analysis

458

Amino Acids

49.31

Weight (kDa)

5.72

Isoelectric Point (pI)

42.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBS	PF00571	305 - 353	1.3e-07	CBS domain
CBS	PF00571	387 - 434	7.9e-08	CBS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69800 AT1G69800 AT1G69800
fragaria_vesca	FvH4_4g31760 FvH4_4g31760 FvH4_4g31760 FvH4_4g31760 FvH4_4g31780 FvH4_4g31780
malus_domestica	MD13G1077700.v1.1 MD13G1077800.v1.1 MD16G1077600.v1.1
prunus_persica	Prupe.1G269400_v2.0.a1
pyrus_communis	pycom13g06740 pycom16g06700
rosa_chinensis	RchiOBHm_Chr4g0440361 RchiOBHm_Chr4g0440371
rosa_laevigata	RLG00000006187 RLG00000006188
rosa_multiflora	Rmu_sc0001685.1_g000046
rosa_roxburghii	Rroxscaffold_5G00381240 Rroxscaffold_5G00381250
rosa_rugosa	Rorug04G0325800 Rorug04G0325800
rosa_samantha	Rh4AG376900 Rh4AG377000 Rh4BG389900 Rh4BG390000 Rh4CG404700 Rh4CG404800 Rh4DG383600 Rh4DG383700
rosa_wichuraiana	Rw4G032270 Rw4G032280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	963
AatII	GACGTC	1 cut(s)	1293
Acc65I	GGTACC	1 cut(s)	306
AccB1I	GGYRCC	3 cut(s)	306, 377, 440
AccI	GTMKAC	1 cut(s)	1184
AccII	CGCG	2 cut(s)	828, 1241
AciI	CCGC	5 cut(s)	104, 516, 828, 1241, 1250
AclWI	GGATC	3 cut(s)	285, 897, 1241
AcoI	YGGCCR	1 cut(s)	781
AcsI	RAATTY	2 cut(s)	30, 1112
AcuI	CTGAAG	2 cut(s)	155, 393
AcyI	GRCGYC	2 cut(s)	441, 1290
AfaI	GTAC	1 cut(s)	308
AfiI	CCNNNNNNNGG	4 cut(s)	587, 1092, 1230, 1341
AflII	CTTAAG	1 cut(s)	794
AflIII	ACRYGT	1 cut(s)	1059
AgsI	TTSAA	3 cut(s)	116, 141, 836
AjnI	CCWGG	2 cut(s)	787, 1144
Alw21I	GWGCWC	1 cut(s)	243
Alw26I	GTCTC	1 cut(s)	1281
AlwI	GGATC	3 cut(s)	285, 897, 1241
AlwNI	CAGNNNCTG	2 cut(s)	434, 476
AoxI	GGCC	2 cut(s)	490, 781
ApeKI	GCWGC	8 cut(s)	11, 57, 368, 398, 479, 500, 503, 554
ApoI	RAATTY	2 cut(s)	30, 1112
Asp700I	GAANNNNTTC	1 cut(s)	81
Asp718I	GGTACC	1 cut(s)	306
AspLEI	GCGC	1 cut(s)	443
AspS9I	GGNCC	1 cut(s)	473
AsuC2I	CCSGG	1 cut(s)	526
AsuHPI	GGTGA	2 cut(s)	274, 602
AsuII	TTCGAA	1 cut(s)	75
AvaII	GGWCC	1 cut(s)	473
BalI	TGGCCA	1 cut(s)	783
BanI	GGYRCC	3 cut(s)	306, 377, 440
BbsI	GAAGAC	1 cut(s)	986
Bbv12I	GWGCWC	1 cut(s)	243
BbvI	GCAGC	8 cut(s)	23, 44, 355, 410, 466, 487, 490, 541
BccI	CCATC	3 cut(s)	734, 1133, 1163
BceAI	ACGGC	2 cut(s)	317, 423
BciT130I	CCWGG	2 cut(s)	789, 1146
BciVI	GTATCC	1 cut(s)	941
BcnI	CCSGG	1 cut(s)	526
BcoDI	GTCTC	1 cut(s)	1281
BfaI	CTAG	2 cut(s)	390, 1025
BfmI	CTRYAG	4 cut(s)	453, 504, 519, 555
BfoI	RGCGCY	1 cut(s)	444
BfrI	CTTAAG	1 cut(s)	794
BfuI	GTATCC	1 cut(s)	941
BglII	AGATCT	1 cut(s)	607
BisI	GCNGC	8 cut(s)	12, 58, 369, 399, 480, 501, 504, 555
BlsI	GCNGC	8 cut(s)	13, 59, 370, 400, 481, 502, 505, 556
Bme1390I	CCNGG	3 cut(s)	526, 789, 1146
Bme18I	GGWCC	1 cut(s)	473
BmgT120I	GGNCC	1 cut(s)	473
BmiI	GGNNCC	3 cut(s)	308, 379, 442
BmrFI	CCNGG	3 cut(s)	526, 789, 1146
BmsI	GCATC	4 cut(s)	55, 208, 830, 1321
BpiI	GAAGAC	1 cut(s)	986
Bpu14I	TTCGAA	1 cut(s)	75
BpuMI	CCSGG	1 cut(s)	526
BsaHI	GRCGYC	2 cut(s)	441, 1290
BsaJI	CCNNGG	2 cut(s)	310, 405
BsaWI	WCCGGW	2 cut(s)	259, 697
BsaXI	ACNNNNNCTCC	4 cut(s)	168, 198, 1062, 1092
Bsc4I	CCNNNNNNNGG	4 cut(s)	587, 1092, 1230, 1341
Bse1I	ACTGG	2 cut(s)	149, 998
Bse3DI	GCAATG	1 cut(s)	867
BseBI	CCWGG	2 cut(s)	789, 1146
BseDI	CCNNGG	2 cut(s)	310, 405
BseGI	GGATG	3 cut(s)	759, 845, 1155
BseLI	CCNNNNNNNGG	4 cut(s)	587, 1092, 1230, 1341
BseMI	GCAATG	1 cut(s)	867
BseMII	CTCAG	2 cut(s)	249, 1297
BseNI	ACTGG	2 cut(s)	149, 998
BseRI	GAGGAG	1 cut(s)	1070
BseXI	GCAGC	8 cut(s)	23, 44, 355, 410, 466, 487, 490, 541
BsgI	GTGCAG	2 cut(s)	378, 447
Bsh1236I	CGCG	2 cut(s)	828, 1241
BshFI	GGCC	2 cut(s)	492, 783
BshNI	GGYRCC	3 cut(s)	306, 377, 440
BsiHKAI	GWGCWC	1 cut(s)	243
BsiSI	CCGG	4 cut(s)	260, 275, 525, 698
BslFI	GGGAC	2 cut(s)	909, 1023
BslI	CCNNNNNNNGG	4 cut(s)	587, 1092, 1230, 1341
BsmAI	GTCTC	1 cut(s)	1281
BsmBI	CGTCTC	1 cut(s)	1281
BsmFI	GGGAC	2 cut(s)	909, 1023
BsnI	GGCC	2 cut(s)	492, 783
Bsp119I	TTCGAA	1 cut(s)	75
Bsp1286I	GDGCHC	1 cut(s)	243
Bsp143I	GATC	6 cut(s)	277, 607, 889, 943, 1075, 1233
BspACI	CCGC	5 cut(s)	104, 516, 828, 1241, 1250
BspANI	GGCC	2 cut(s)	492, 783
BspCNI	CTCAG	2 cut(s)	248, 1296
BspFNI	CGCG	2 cut(s)	828, 1241
BspLI	GGNNCC	3 cut(s)	308, 379, 442
BspMAI	CTGCAG	2 cut(s)	508, 559
BspPI	GGATC	3 cut(s)	285, 897, 1241
BspT104I	TTCGAA	1 cut(s)	75
BspT107I	GGYRCC	3 cut(s)	306, 377, 440
BspTI	CTTAAG	1 cut(s)	794
BsrDI	GCAATG	1 cut(s)	867
BsrI	ACTGG	2 cut(s)	149, 998
BssECI	CCNNGG	2 cut(s)	310, 405
BssMI	GATC	6 cut(s)	277, 607, 889, 943, 1075, 1233
BssNI	GRCGYC	2 cut(s)	441, 1290
BssT1I	CCWWGG	1 cut(s)	310
Bst2UI	CCWGG	2 cut(s)	789, 1146
Bst4CI	ACNGT	2 cut(s)	271, 655
BstACI	GRCGYC	2 cut(s)	441, 1290
BstAFI	CTTAAG	1 cut(s)	794
BstAPI	GCANNNNNTGC	1 cut(s)	365
BstBI	TTCGAA	1 cut(s)	75
BstC8I	GCNNGC	1 cut(s)	490
BstDEI	CTNAG	3 cut(s)	45, 235, 1283
BstDSI	CCRYGG	1 cut(s)	405
BstF5I	GGATG	3 cut(s)	759, 845, 1155
BstFNI	CGCG	2 cut(s)	828, 1241
BstH2I	RGCGCY	1 cut(s)	444
BstHHI	GCGC	1 cut(s)	443
BstKTI	GATC	6 cut(s)	280, 610, 892, 946, 1078, 1236
BstMAI	GTCTC	1 cut(s)	1281
BstMBI	GATC	6 cut(s)	277, 607, 889, 943, 1075, 1233
BstNI	CCWGG	2 cut(s)	789, 1146
BstNSI	RCATGY	3 cut(s)	368, 1063, 1173
BstSCI	CCNGG	3 cut(s)	524, 787, 1144
BstSFI	CTRYAG	4 cut(s)	453, 504, 519, 555
BstUI	CGCG	2 cut(s)	828, 1241
BstV1I	GCAGC	8 cut(s)	23, 44, 355, 410, 466, 487, 490, 541
BstV2I	GAAGAC	1 cut(s)	986
BstX2I	RGATCY	3 cut(s)	607, 889, 1233
BstYI	RGATCY	3 cut(s)	607, 889, 1233
BsuI	GTATCC	1 cut(s)	941
BsuRI	GGCC	2 cut(s)	492, 783
BtgI	CCRYGG	1 cut(s)	405
BtsCI	GGATG	3 cut(s)	759, 845, 1155
BtsI	GCAGTG	2 cut(s)	1101, 1356
BtsIMutI	CAGTG	2 cut(s)	1101, 1356
Cac8I	GCNNGC	1 cut(s)	490
CaiI	CAGNNNCTG	2 cut(s)	434, 476
CfoI	GCGC	1 cut(s)	443
Cfr13I	GGNCC	1 cut(s)	473
Csp6I	GTAC	1 cut(s)	307
CviAII	CATG	4 cut(s)	107, 365, 1060, 1170
CviQI	GTAC	1 cut(s)	307
DdeI	CTNAG	3 cut(s)	45, 235, 1283
DinI	GGCGCC	1 cut(s)	442
DpnI	GATC	6 cut(s)	279, 609, 891, 945, 1077, 1235
DpnII	GATC	6 cut(s)	277, 607, 889, 943, 1075, 1233
EaeI	YGGCCR	1 cut(s)	781
Eco130I	CCWWGG	1 cut(s)	310
Eco147I	AGGCCT	1 cut(s)	492
Eco32I	GATATC	1 cut(s)	1051
Eco47I	GGWCC	1 cut(s)	473
Eco57I	CTGAAG	2 cut(s)	155, 393
EcoO109I	RGGNCCY	1 cut(s)	473
EcoRI	GAATTC	2 cut(s)	30, 1112
EcoRII	CCWGG	2 cut(s)	787, 1144
EcoRV	GATATC	1 cut(s)	1051
EcoT14I	CCWWGG	1 cut(s)	310
EcoT22I	ATGCAT	1 cut(s)	136
EgeI	GGCGCC	1 cut(s)	442
EheI	GGCGCC	1 cut(s)	442
ErhI	CCWWGG	1 cut(s)	310
Esp3I	CGTCTC	1 cut(s)	1281
FaeI	CATG	4 cut(s)	110, 368, 1063, 1173
FaqI	GGGAC	2 cut(s)	909, 1023
FatI	CATG	4 cut(s)	106, 364, 1059, 1169
FblI	GTMKAC	1 cut(s)	1184
Fnu4HI	GCNGC	8 cut(s)	12, 58, 369, 399, 480, 501, 504, 555
FokI	GGATG	3 cut(s)	766, 852, 1142
Fsp4HI	GCNGC	8 cut(s)	12, 58, 369, 399, 480, 501, 504, 555
FspBI	CTAG	2 cut(s)	390, 1025
GlaI	GCGC	1 cut(s)	442
GluI	GCNGC	8 cut(s)	12, 58, 369, 399, 480, 501, 504, 555
HaeII	RGCGCY	1 cut(s)	444
HaeIII	GGCC	2 cut(s)	492, 783
HapII	CCGG	4 cut(s)	260, 275, 525, 698
HhaI	GCGC	1 cut(s)	443
Hin1I	GRCGYC	2 cut(s)	441, 1290
Hin1II	CATG	4 cut(s)	110, 368, 1063, 1173
Hin6I	GCGC	1 cut(s)	441
HinP1I	GCGC	1 cut(s)	441
HincII	GTYRAC	3 cut(s)	648, 723, 1185
HindII	GTYRAC	3 cut(s)	648, 723, 1185
HindIII	AAGCTT	1 cut(s)	957
HinfI	GANTC	8 cut(s)	40, 82, 229, 315, 575, 1181, 1186, 1207
HpaII	CCGG	4 cut(s)	260, 275, 525, 698
HphI	GGTGA	2 cut(s)	274, 602
Hpy166II	GTNNAC	3 cut(s)	648, 723, 1185
Hpy188I	TCNGA	7 cut(s)	238, 292, 889, 916, 948, 1055, 1286
Hpy188III	TCNNGA	8 cut(s)	91, 116, 215, 226, 611, 833, 934, 1093
Hpy8I	GTNNAC	3 cut(s)	648, 723, 1185
HpyCH4III	ACNGT	2 cut(s)	271, 655
HpyCH4IV	ACGT	2 cut(s)	128, 1290
HpyF3I	CTNAG	3 cut(s)	45, 235, 1283
HpySE526I	ACGT	2 cut(s)	128, 1290
Hsp92I	GRCGYC	2 cut(s)	441, 1290
Hsp92II	CATG	4 cut(s)	110, 368, 1063, 1173
HspAI	GCGC	1 cut(s)	441
KasI	GGCGCC	1 cut(s)	440
KpnI	GGTACC	1 cut(s)	310
Kzo9I	GATC	6 cut(s)	277, 607, 889, 943, 1075, 1233
LmnI	GCTCC	3 cut(s)	262, 431, 449
Lsp1109I	GCAGC	8 cut(s)	23, 44, 355, 410, 466, 487, 490, 541
LweI	GCATC	4 cut(s)	55, 208, 830, 1321
MaeI	CTAG	2 cut(s)	390, 1025
MaeII	ACGT	2 cut(s)	128, 1290
MaeIII	GTNAC	1 cut(s)	466
MalI	GATC	6 cut(s)	279, 609, 891, 945, 1077, 1235
MboI	GATC	6 cut(s)	277, 607, 889, 943, 1075, 1233
MboII	GAAGA	5 cut(s)	106, 719, 986, 1030, 1265
MfeI	CAATTG	1 cut(s)	483
MflI	RGATCY	3 cut(s)	607, 889, 1233
MhlI	GDGCHC	1 cut(s)	243
MlsI	TGGCCA	1 cut(s)	783
MluNI	TGGCCA	1 cut(s)	783
Mly113I	GGCGCC	1 cut(s)	441
MlyI	GAGTC	4 cut(s)	49, 223, 1180, 1190
MmeI	TCCRAC	2 cut(s)	330, 517
Mox20I	TGGCCA	1 cut(s)	783
Mph1103I	ATGCAT	1 cut(s)	136
MroXI	GAANNNNTTC	1 cut(s)	81
MscI	TGGCCA	1 cut(s)	783
MseI	TTAA	3 cut(s)	125, 669, 795
MslI	CAYNNNNRTG	2 cut(s)	1106, 1329
Msp20I	TGGCCA	1 cut(s)	783
MspA1I	CMGCKG	1 cut(s)	518
MspCI	CTTAAG	1 cut(s)	794
MspI	CCGG	4 cut(s)	260, 275, 525, 698
MspR9I	CCNGG	3 cut(s)	526, 789, 1146
MunI	CAATTG	1 cut(s)	483
MvaI	CCWGG	2 cut(s)	789, 1146
MvnI	CGCG	2 cut(s)	828, 1241
NarI	GGCGCC	1 cut(s)	441
NciI	CCSGG	1 cut(s)	526
NdeII	GATC	6 cut(s)	277, 607, 889, 943, 1075, 1233
NlaIII	CATG	4 cut(s)	110, 368, 1063, 1173
NlaIV	GGNNCC	3 cut(s)	308, 379, 442
NmuCI	GTSAC	1 cut(s)	466
NsiI	ATGCAT	1 cut(s)	136
NspI	RCATGY	3 cut(s)	368, 1063, 1173
NspV	TTCGAA	1 cut(s)	75
PceI	AGGCCT	1 cut(s)	492
PciI	ACATGT	1 cut(s)	1059
PdmI	GAANNNNTTC	1 cut(s)	81
PfeI	GAWTC	4 cut(s)	82, 315, 575, 1207
PflFI	GACNNNGTC	1 cut(s)	617
PkrI	GCNGC	8 cut(s)	13, 59, 370, 400, 481, 502, 505, 556
PleI	GAGTC	4 cut(s)	48, 223, 1180, 1189
PluTI	GGCGCC	1 cut(s)	444
PpsI	GAGTC	4 cut(s)	48, 223, 1180, 1189
PpuMI	RGGWCCY	1 cut(s)	473
PscI	ACATGT	1 cut(s)	1059
PsiI	TTATAA	1 cut(s)	963
Psp5II	RGGWCCY	1 cut(s)	473
Psp6I	CCWGG	2 cut(s)	787, 1144
PspGI	CCWGG	2 cut(s)	787, 1144
PspN4I	GGNNCC	3 cut(s)	308, 379, 442
PspPI	GGNCC	1 cut(s)	473
PspPPI	RGGWCCY	1 cut(s)	473
PstI	CTGCAG	2 cut(s)	508, 559
PstNI	CAGNNNCTG	2 cut(s)	434, 476
PsuI	RGATCY	3 cut(s)	607, 889, 1233
PsyI	GACNNNGTC	1 cut(s)	617
RsaI	GTAC	1 cut(s)	308
RsaNI	GTAC	1 cut(s)	307
RseI	CAYNNNNRTG	2 cut(s)	1106, 1329
SalI	GTCGAC	1 cut(s)	1183
SaqAI	TTAA	3 cut(s)	125, 669, 795
SatI	GCNGC	8 cut(s)	12, 58, 369, 399, 480, 501, 504, 555
Sau3AI	GATC	6 cut(s)	277, 607, 889, 943, 1075, 1233
Sau96I	GGNCC	1 cut(s)	473
SchI	GAGTC	4 cut(s)	49, 223, 1180, 1190
ScrFI	CCNGG	3 cut(s)	526, 789, 1146
SduI	GDGCHC	1 cut(s)	243
SfaNI	GCATC	4 cut(s)	55, 208, 830, 1321
SfcI	CTRYAG	4 cut(s)	453, 504, 519, 555
SfoI	GGCGCC	1 cut(s)	442
SfuI	TTCGAA	1 cut(s)	75
SinI	GGWCC	1 cut(s)	473
SmiMI	CAYNNNNRTG	2 cut(s)	1106, 1329
SmlI	CTYRAG	1 cut(s)	794
SmoI	CTYRAG	1 cut(s)	794
SseBI	AGGCCT	1 cut(s)	492
SsiI	CCGC	5 cut(s)	104, 516, 828, 1241, 1250
SspDI	GGCGCC	1 cut(s)	440
SspMI	CTAG	2 cut(s)	390, 1025
StuI	AGGCCT	1 cut(s)	492
StyD4I	CCNGG	3 cut(s)	524, 787, 1144
StyI	CCWWGG	1 cut(s)	310
TaaI	ACNGT	2 cut(s)	271, 655
TaiI	ACGT	2 cut(s)	131, 1293
TaqI	TCGA	3 cut(s)	75, 1179, 1184
TfiI	GAWTC	4 cut(s)	82, 315, 575, 1207
Tru1I	TTAA	3 cut(s)	125, 669, 795
Tru9I	TTAA	3 cut(s)	125, 669, 795
TscAI	CASTG	2 cut(s)	1108, 1356
TseFI	GTSAC	1 cut(s)	466
TseI	GCWGC	8 cut(s)	11, 57, 368, 398, 479, 500, 503, 554
Tsp45I	GTSAC	1 cut(s)	466
TspDTI	ATGAA	6 cut(s)	144, 648, 986, 1217, 1294, 1326
TspGWI	ACGGA	1 cut(s)	1213
TspRI	CASTG	2 cut(s)	1108, 1356
Tth111I	GACNNNGTC	1 cut(s)	617
Vha464I	CTTAAG	1 cut(s)	794
VpaK11BI	GGWCC	1 cut(s)	473
XapI	RAATTY	2 cut(s)	30, 1112
XceI	RCATGY	3 cut(s)	368, 1063, 1173
XmiI	GTMKAC	1 cut(s)	1184
XmnI	GAANNNNTTC	1 cut(s)	81
XspI	CTAG	2 cut(s)	390, 1025
ZraI	GACGTC	1 cut(s)	1291
Zsp2I	ATGCAT	1 cut(s)	136

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.