Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005047

Physical Location & Seq

Genomic Coordinates Reverse (-)

26485238 .. 26487595

Gene Structure 2358 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G70310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1023 bp

ATGTCTTCAACACAAGAAGCGTCTGTTACTGATTTGCCCGTGAAGAGACCTAGAGAAGCAGAGGAGGACAATAACGGCGGCGCCATGGAAACAGAGAACGGTGGAGGAGAGATAAAGGAGCCTTCTTGTATGTCCTCTATTATTCCTGGATGGTTCTCTGAGATTAGTCCTATGTGGCCAGGAGAAGCACATTCTCTCAAGGTAGAGAAGATTCTATTCCAAGGGAAATCAGATTACCAGGATGTTATTGTTTTCCAGTCTGCAACATATGGAAAGGTTTTGGTTTTGGATGGAGTGATTCAACTCACTGAGAGAGATGAATGTGCGTATCAAGAAATGATCACTCATCTTCCTTTGTGCTCTATCTCCAACCCCAAAAAGGTACTGGTGATTGGAGGAGGAGATGGAGGAGTCCTGAGGGAAGTGGCACGTCATAGTTCTGTTGAGCAGATTGACATTTGTGAAATAGATAAAATGGTGGTTGATGTGGCTAAGCAGTATTTCCCTAATGTAGCAGTTGGATACGAGGATCCTCGTGTCAACCTCATCATTGGCGATGGTGTTGCTTTCTTGAAGAACGCTGCTGAAGGAACCTATGATGCAGTTATTGTTGATTCATCTGATCCAATCGGTCCAGCAAAAGAGCTATTTGAGAAACCTTTCTTTGAGTCAGTGAATAGAGCTCTTCGTCCTGGTGGAGTTGTGTGCACACAAGCTGAAAGCTTGTGGCTTCACATGGATATCATTGAAGACATTGTTTCTAATTGCCGTGACATCTTTAAAGGATCTGTTAACTACGCTTGGACCAGTGTTCCAACTTACCCGAGTGGAGTCATTGGATTCATGCTTTGTTCATCTGAAGGACCACAAGTCGATTTCAAGAAGCCAGTGAGTCTAATCGATACTGATGAAAGCTCTATCAAATCACACTGTCCCTTGAAGTATTACAACGCTGAGATTCACTCAGCTGCTTTCTGCTTGCCCTCTTTTGCTAAGAAGGTGATTGATTCGAAAGCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004766 GO:0005575 GO:0005623 GO:0005886 GO:0006576 GO:0006595 GO:0006596 GO:0006807 GO:0008150 GO:0008152 GO:0008216 GO:0008295 GO:0009058 GO:0009308 GO:0009309 GO:0009987 GO:0016020 GO:0016740 GO:0016765 GO:0034641 GO:0042401 GO:0044106 GO:0044237 GO:0044249 GO:0044271 GO:0044464 GO:0071704 GO:0071944 GO:0097164 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00270 ko00330 ko00410 ko00480 ko00960 ko01100 ko01110 map00270 map00330 map00410 map00480 map00960 map01100 map01110

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

340

Amino Acids

37.14

Weight (kDa)

4.72

Isoelectric Point (pI)

44.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	50 - 104	1.7e-24	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	107 - 295	4.8e-78	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005047)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23820 AT1G23820 AT1G70310
fragaria_vesca	FvH4_4g34680
malus_domestica	MD13G1090400.v1.1 MD14G1183600.v1.1 MD16G1091000.v1.1
prunus_persica	Prupe.1G255300_v2.0.a1 Prupe.5G176100_v2.0.a1
pyrus_communis	pycom13g07870 pycom16g07770
rosa_chinensis	RchiOBHm_Chr4g0444031 RchiOBHm_Chr7g0193881
rosa_laevigata	RLG00000004238 RLG00000005863
rosa_multiflora	Rmu_sc0015522.1_g000006
rosa_roxburghii	Rroxscaffold_3G00261760 Rroxscaffold_5G00384600
rosa_rugosa	Rorug04G0349100 Rorug07G0012500
rosa_samantha	Rh4AG409900 Rh4BG421000 Rh4CG435300 Rh4DG416200 Rh7AG139200 Rh7BG139600 Rh7CG143100 Rh7DG140700
rosa_wichuraiana	Rw4G035210 Rw7G011880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	80
AciI	CCGC	1 cut(s)	78
AclWI	GGATC	4 cut(s)	524, 537, 617, 793
AcoI	YGGCCR	1 cut(s)	176
AcuI	CTGAAG	2 cut(s)	606, 879
AcyI	GRCGYC	1 cut(s)	81
AfaI	GTAC	1 cut(s)	384
AfiI	CCNNNNNNNGG	1 cut(s)	379
AgsI	TTSAA	6 cut(s)	9, 302, 574, 749, 880, 940
AhdI	GACNNNNNGTC	1 cut(s)	869
AjiI	CACGTC	1 cut(s)	431
AjnI	CCWGG	4 cut(s)	145, 178, 237, 691
AluBI	AGCT	6 cut(s)	646, 683, 716, 723, 915, 968
AluI	AGCT	6 cut(s)	646, 683, 716, 723, 915, 968
Alw21I	GWGCWC	3 cut(s)	362, 685, 710
Alw26I	GTCTC	1 cut(s)	40
Alw44I	GTGCAC	1 cut(s)	706
AlwI	GGATC	4 cut(s)	524, 537, 617, 793
Ama87I	CYCGRG	1 cut(s)	823
AoxI	GGCC	1 cut(s)	176
ApaLI	GTGCAC	1 cut(s)	706
ApeKI	GCWGC	2 cut(s)	581, 968
Asp700I	GAANNNNTTC	1 cut(s)	659
AspLEI	GCGC	1 cut(s)	83
AspS9I	GGNCC	3 cut(s)	632, 804, 863
AsuHPI	GGTGA	2 cut(s)	400, 1012
AsuII	TTCGAA	1 cut(s)	1010
AvaI	CYCGRG	1 cut(s)	823
AvaII	GGWCC	3 cut(s)	632, 804, 863
AxyI	CCTNAGG	1 cut(s)	416
BaeGI	GKGCMC	1 cut(s)	710
BalI	TGGCCA	1 cut(s)	178
BamHI	GGATCC	1 cut(s)	529
BanI	GGYRCC	1 cut(s)	80
BanII	GRGCYC	1 cut(s)	685
BauI	CACGAG	1 cut(s)	534
BbsI	GAAGAC	1 cut(s)	756
Bbv12I	GWGCWC	3 cut(s)	362, 685, 710
BbvI	GCAGC	2 cut(s)	568, 955
BccI	CCATC	4 cut(s)	144, 284, 398, 551
BceAI	ACGGC	2 cut(s)	91, 753
BcgI	CGANNNNNNTGC	2 cut(s)	545, 579
BciT130I	CCWGG	4 cut(s)	147, 180, 239, 693
BciVI	GTATCC	1 cut(s)	515
BclI	TGATCA	1 cut(s)	339
BcoDI	GTCTC	1 cut(s)	40
BfaI	CTAG	2 cut(s)	51, 1021
BfoI	RGCGCY	1 cut(s)	84
BfuI	GTATCC	1 cut(s)	515
BisI	GCNGC	3 cut(s)	79, 582, 969
BlpI	GCTNAGC	1 cut(s)	492
BlsI	GCNGC	3 cut(s)	80, 583, 970
Bme1390I	CCNGG	4 cut(s)	147, 180, 239, 693
Bme18I	GGWCC	3 cut(s)	632, 804, 863
BmeRI	GACNNNNNGTC	1 cut(s)	869
BmeT110I	CYCGRG	1 cut(s)	823
BmgBI	CACGTC	1 cut(s)	431
BmgT120I	GGNCC	3 cut(s)	632, 804, 863
BmiI	GGNNCC	4 cut(s)	82, 120, 531, 592
BmrFI	CCNGG	4 cut(s)	147, 180, 239, 693
BmsI	GCATC	1 cut(s)	589
BpiI	GAAGAC	1 cut(s)	756
BplI	GAGNNNNNCTC	2 cut(s)	947, 979
Bpu1102I	GCTNAGC	1 cut(s)	492
Bpu14I	TTCGAA	1 cut(s)	1010
BpuEI	CTTGAG	1 cut(s)	182
Bsa29I	ATCGAT	1 cut(s)	900
BsaHI	GRCGYC	1 cut(s)	81
BsaI	GGTCTC	1 cut(s)	40
BsaJI	CCNNGG	2 cut(s)	84, 220
Bsc4I	CCNNNNNNNGG	1 cut(s)	379
Bse1I	ACTGG	4 cut(s)	256, 390, 807, 887
Bse21I	CCTNAGG	1 cut(s)	416
BseBI	CCWGG	4 cut(s)	147, 180, 239, 693
BseCI	ATCGAT	1 cut(s)	900
BseDI	CCNNGG	2 cut(s)	84, 220
BseGI	GGATG	3 cut(s)	155, 247, 295
BseLI	CCNNNNNNNGG	1 cut(s)	379
BseMII	CTCAG	5 cut(s)	150, 300, 407, 945, 978
BseNI	ACTGG	4 cut(s)	256, 390, 807, 887
BseRI	GAGGAG	5 cut(s)	77, 120, 411, 414, 423
BseSI	GKGCMC	1 cut(s)	710
BseXI	GCAGC	2 cut(s)	568, 955
BshFI	GGCC	1 cut(s)	178
BshNI	GGYRCC	1 cut(s)	80
BshVI	ATCGAT	1 cut(s)	900
BsiHKAI	GWGCWC	3 cut(s)	362, 685, 710
BsiHKCI	CYCGRG	1 cut(s)	823
BslFI	GGGAC	1 cut(s)	918
BslI	CCNNNNNNNGG	1 cut(s)	379
BsmAI	GTCTC	1 cut(s)	40
BsmFI	GGGAC	1 cut(s)	918
BsnI	GGCC	1 cut(s)	178
Bso31I	GGTCTC	1 cut(s)	40
BsoBI	CYCGRG	1 cut(s)	823
Bsp119I	TTCGAA	1 cut(s)	1010
Bsp1286I	GDGCHC	3 cut(s)	362, 685, 710
Bsp143I	GATC	4 cut(s)	339, 529, 622, 785
Bsp1720I	GCTNAGC	1 cut(s)	492
Bsp19I	CCATGG	1 cut(s)	84
BspACI	CCGC	1 cut(s)	78
BspANI	GGCC	1 cut(s)	178
BspCNI	CTCAG	5 cut(s)	151, 301, 408, 946, 977
BspDI	ATCGAT	1 cut(s)	900
BspLI	GGNNCC	4 cut(s)	82, 120, 531, 592
BspPI	GGATC	4 cut(s)	524, 537, 617, 793
BspQI	GCTCTTC	1 cut(s)	690
BspT104I	TTCGAA	1 cut(s)	1010
BspT107I	GGYRCC	1 cut(s)	80
BspTNI	GGTCTC	1 cut(s)	40
BsrI	ACTGG	4 cut(s)	256, 390, 807, 887
BssECI	CCNNGG	2 cut(s)	84, 220
BssMI	GATC	4 cut(s)	339, 529, 622, 785
BssNI	GRCGYC	1 cut(s)	81
BssSI	CACGAG	1 cut(s)	534
BssT1I	CCWWGG	2 cut(s)	84, 220
Bst2BI	CACGAG	1 cut(s)	534
Bst2UI	CCWGG	4 cut(s)	147, 180, 239, 693
Bst4CI	ACNGT	2 cut(s)	101, 932
Bst6I	CTCTTC	2 cut(s)	38, 690
BstACI	GRCGYC	1 cut(s)	81
BstBI	TTCGAA	1 cut(s)	1010
BstC8I	GCNNGC	1 cut(s)	980
BstDEI	CTNAG	7 cut(s)	159, 309, 416, 492, 954, 964, 993
BstDSI	CCRYGG	1 cut(s)	84
BstF5I	GGATG	3 cut(s)	155, 247, 295
BstH2I	RGCGCY	1 cut(s)	84
BstHHI	GCGC	1 cut(s)	83
BstKTI	GATC	4 cut(s)	342, 532, 625, 788
BstMAI	GTCTC	1 cut(s)	40
BstMBI	GATC	4 cut(s)	339, 529, 622, 785
BstNI	CCWGG	4 cut(s)	147, 180, 239, 693
BstSCI	CCNGG	4 cut(s)	145, 178, 237, 691
BstSLI	GKGCMC	1 cut(s)	710
BstV1I	GCAGC	2 cut(s)	568, 955
BstV2I	GAAGAC	1 cut(s)	756
BstX2I	RGATCY	2 cut(s)	529, 785
BstYI	RGATCY	2 cut(s)	529, 785
Bsu15I	ATCGAT	1 cut(s)	900
Bsu36I	CCTNAGG	1 cut(s)	416
BsuI	GTATCC	1 cut(s)	515
BsuRI	GGCC	1 cut(s)	178
BsuTUI	ATCGAT	1 cut(s)	900
BtgI	CCRYGG	1 cut(s)	84
BtgZI	GCGATG	1 cut(s)	570
BtrI	CACGTC	1 cut(s)	431
BtsCI	GGATG	3 cut(s)	155, 247, 295
BtsIMutI	CAGTG	5 cut(s)	306, 678, 814, 894, 928
Cac8I	GCNNGC	1 cut(s)	980
CfoI	GCGC	1 cut(s)	83
Cfr13I	GGNCC	3 cut(s)	632, 804, 863
ClaI	ATCGAT	1 cut(s)	900
CseI	GACGC	1 cut(s)	9
Csp6I	GTAC	1 cut(s)	383
CviAII	CATG	3 cut(s)	85, 736, 844
CviQI	GTAC	1 cut(s)	383
DdeI	CTNAG	7 cut(s)	159, 309, 416, 492, 954, 964, 993
DinI	GGCGCC	1 cut(s)	82
DpnI	GATC	4 cut(s)	341, 531, 624, 787
DpnII	GATC	4 cut(s)	339, 529, 622, 785
DraI	TTTAAA	1 cut(s)	781
DriI	GACNNNNNGTC	1 cut(s)	869
EaeI	YGGCCR	1 cut(s)	176
Eam1104I	CTCTTC	2 cut(s)	38, 690
Eam1105I	GACNNNNNGTC	1 cut(s)	869
EarI	CTCTTC	2 cut(s)	38, 690
Ecl136II	GAGCTC	1 cut(s)	683
Eco130I	CCWWGG	2 cut(s)	84, 220
Eco24I	GRGCYC	1 cut(s)	685
Eco31I	GGTCTC	1 cut(s)	40
Eco32I	GATATC	1 cut(s)	742
Eco47I	GGWCC	3 cut(s)	632, 804, 863
Eco53kI	GAGCTC	1 cut(s)	683
Eco57I	CTGAAG	2 cut(s)	606, 879
Eco81I	CCTNAGG	1 cut(s)	416
Eco88I	CYCGRG	1 cut(s)	823
EcoICRI	GAGCTC	1 cut(s)	683
EcoRII	CCWGG	4 cut(s)	145, 178, 237, 691
EcoRV	GATATC	1 cut(s)	742
EcoT14I	CCWWGG	2 cut(s)	84, 220
EcoT38I	GRGCYC	1 cut(s)	685
EgeI	GGCGCC	1 cut(s)	82
EheI	GGCGCC	1 cut(s)	82
ErhI	CCWWGG	2 cut(s)	84, 220
FaeI	CATG	3 cut(s)	88, 739, 847
FaiI	YATR	9 cut(s)	86, 131, 173, 268, 270, 435, 597, 737, 845
FaqI	GGGAC	1 cut(s)	918
FatI	CATG	3 cut(s)	84, 735, 843
FauNDI	CATATG	1 cut(s)	268
FbaI	TGATCA	1 cut(s)	339
Fnu4HI	GCNGC	3 cut(s)	79, 582, 969
FokI	GGATG	3 cut(s)	162, 254, 302
FriOI	GRGCYC	1 cut(s)	685
Fsp4HI	GCNGC	3 cut(s)	79, 582, 969
FspBI	CTAG	2 cut(s)	51, 1021
GlaI	GCGC	1 cut(s)	82
GluI	GCNGC	3 cut(s)	79, 582, 969
HaeII	RGCGCY	1 cut(s)	84
HaeIII	GGCC	1 cut(s)	178
HgaI	GACGC	1 cut(s)	9
HhaI	GCGC	1 cut(s)	83
Hin1I	GRCGYC	1 cut(s)	81
Hin1II	CATG	3 cut(s)	88, 739, 847
Hin6I	GCGC	1 cut(s)	81
HinP1I	GCGC	1 cut(s)	81
HincII	GTYRAC	2 cut(s)	541, 793
HindII	GTYRAC	2 cut(s)	541, 793
HindIII	AAGCTT	1 cut(s)	721
HpaI	GTTAAC	1 cut(s)	793
HphI	GGTGA	2 cut(s)	400, 1012
Hpy166II	GTNNAC	3 cut(s)	541, 708, 793
Hpy188I	TCNGA	4 cut(s)	160, 232, 622, 859
Hpy188III	TCNNGA	4 cut(s)	332, 415, 571, 880
Hpy8I	GTNNAC	3 cut(s)	541, 708, 793
HpyAV	CCTTC	4 cut(s)	132, 581, 854, 991
HpyCH4III	ACNGT	2 cut(s)	101, 932
HpyCH4IV	ACGT	1 cut(s)	430
HpyCH4V	TGCA	3 cut(s)	263, 602, 708
HpyF3I	CTNAG	7 cut(s)	159, 309, 416, 492, 954, 964, 993
HpySE526I	ACGT	1 cut(s)	430
Hsp92I	GRCGYC	1 cut(s)	81
Hsp92II	CATG	3 cut(s)	88, 739, 847
HspAI	GCGC	1 cut(s)	81
KasI	GGCGCC	1 cut(s)	80
Ksp22I	TGATCA	1 cut(s)	339
KspAI	GTTAAC	1 cut(s)	793
Kzo9I	GATC	4 cut(s)	339, 529, 622, 785
LguI	GCTCTTC	1 cut(s)	690
LmnI	GCTCC	1 cut(s)	118
Lsp1109I	GCAGC	2 cut(s)	568, 955
LweI	GCATC	1 cut(s)	589
MaeI	CTAG	2 cut(s)	51, 1021
MaeII	ACGT	1 cut(s)	430
MaeIII	GTNAC	2 cut(s)	25, 770
MalI	GATC	4 cut(s)	341, 531, 624, 787
MboI	GATC	4 cut(s)	339, 529, 622, 785
MboII	GAAGA	6 cut(s)	55, 220, 341, 586, 677, 761
MflI	RGATCY	2 cut(s)	529, 785
MhlI	GDGCHC	3 cut(s)	362, 685, 710
MlsI	TGGCCA	1 cut(s)	178
MluCI	AATT	1 cut(s)	763
MluNI	TGGCCA	1 cut(s)	178
Mly113I	GGCGCC	1 cut(s)	81
MlyI	GAGTC	4 cut(s)	420, 677, 840, 901
MmeI	TCCRAC	3 cut(s)	393, 499, 839
Mox20I	TGGCCA	1 cut(s)	178
MroXI	GAANNNNTTC	1 cut(s)	659
MscI	TGGCCA	1 cut(s)	178
MseI	TTAA	2 cut(s)	780, 792
Msp20I	TGGCCA	1 cut(s)	178
MspA1I	CMGCKG	1 cut(s)	968
MspR9I	CCNGG	4 cut(s)	147, 180, 239, 693
MvaI	CCWGG	4 cut(s)	147, 180, 239, 693
NarI	GGCGCC	1 cut(s)	81
NcoI	CCATGG	1 cut(s)	84
NdeI	CATATG	1 cut(s)	268
NdeII	GATC	4 cut(s)	339, 529, 622, 785
NlaIII	CATG	3 cut(s)	88, 739, 847
NlaIV	GGNNCC	4 cut(s)	82, 120, 531, 592
NmuCI	GTSAC	1 cut(s)	770
NspV	TTCGAA	1 cut(s)	1010
PciSI	GCTCTTC	1 cut(s)	690
PdmI	GAANNNNTTC	1 cut(s)	659
PfeI	GAWTC	6 cut(s)	211, 298, 614, 840, 958, 1007
PfoI	TCCNGGA	1 cut(s)	145
PkrI	GCNGC	3 cut(s)	80, 583, 970
PleI	GAGTC	4 cut(s)	419, 676, 839, 900
PluTI	GGCGCC	1 cut(s)	84
PpsI	GAGTC	4 cut(s)	419, 676, 839, 900
Psp124BI	GAGCTC	1 cut(s)	685
Psp6I	CCWGG	4 cut(s)	145, 178, 237, 691
PspGI	CCWGG	4 cut(s)	145, 178, 237, 691
PspN4I	GGNNCC	4 cut(s)	82, 120, 531, 592
PspPI	GGNCC	3 cut(s)	632, 804, 863
PsuI	RGATCY	2 cut(s)	529, 785
PvuII	CAGCTG	1 cut(s)	968
RsaI	GTAC	1 cut(s)	384
RsaNI	GTAC	1 cut(s)	383
SacI	GAGCTC	1 cut(s)	685
SapI	GCTCTTC	1 cut(s)	690
SaqAI	TTAA	2 cut(s)	780, 792
SatI	GCNGC	3 cut(s)	79, 582, 969
Sau3AI	GATC	4 cut(s)	339, 529, 622, 785
Sau96I	GGNCC	3 cut(s)	632, 804, 863
SchI	GAGTC	4 cut(s)	420, 677, 840, 901
ScrFI	CCNGG	4 cut(s)	147, 180, 239, 693
SduI	GDGCHC	3 cut(s)	362, 685, 710
SfaNI	GCATC	1 cut(s)	589
SfoI	GGCGCC	1 cut(s)	82
SfuI	TTCGAA	1 cut(s)	1010
SinI	GGWCC	3 cut(s)	632, 804, 863
SmlI	CTYRAG	1 cut(s)	197
SmoI	CTYRAG	1 cut(s)	197
Sse9I	AATT	1 cut(s)	763
SsiI	CCGC	1 cut(s)	78
SspDI	GGCGCC	1 cut(s)	80
SspMI	CTAG	2 cut(s)	51, 1021
SstI	GAGCTC	1 cut(s)	685
StyD4I	CCNGG	4 cut(s)	145, 178, 237, 691
StyI	CCWWGG	2 cut(s)	84, 220
TaaI	ACNGT	2 cut(s)	101, 932
TaiI	ACGT	1 cut(s)	433
TaqI	TCGA	3 cut(s)	873, 900, 1010
TaqII	GACCGA	1 cut(s)	620
TasI	AATT	1 cut(s)	763
TauI	GCSGC	1 cut(s)	81
TfiI	GAWTC	6 cut(s)	211, 298, 614, 840, 958, 1007
Tru1I	TTAA	2 cut(s)	780, 792
Tru9I	TTAA	2 cut(s)	780, 792
TscAI	CASTG	5 cut(s)	313, 678, 814, 894, 935
TseFI	GTSAC	1 cut(s)	770
TseI	GCWGC	2 cut(s)	581, 968
Tsp45I	GTSAC	1 cut(s)	770
TspDTI	ATGAA	5 cut(s)	333, 606, 832, 843, 924
TspRI	CASTG	5 cut(s)	313, 678, 814, 894, 935
VneI	GTGCAC	1 cut(s)	706
VpaK11BI	GGWCC	3 cut(s)	632, 804, 863
XcmI	CCANNNNNNNNNTGG	1 cut(s)	382
XmnI	GAANNNNTTC	1 cut(s)	659
XspI	CTAG	2 cut(s)	51, 1021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.