Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005047

Physical Location & Seq

Genomic Coordinates Reverse (-)

6320235 .. 6324893

Gene Structure 4659 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1091000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1008 bp

ATGGCGGACGAGATTGTGGTGGGGTCTGCTGATTTGCCCTTGAAGAGGCCCAGGGAGGAAGAAGAGAACGGCGCGGAAGCTGCTGCTTCAGCCGTGTCCATGGAGACTGACGGTGGCAAGGAGCCGGATTCTGTCTCCGCTGTTATTCCCGGCTGGTTTTCTGAGATTAGCCCAATGTGGCCAGGAGAGGCACACTCCTTGAAGATAGAGAAGATTTTGTTTCAAGGAAAGTCCGACTATCAGAATGTGATGGTCTTCCAGTCAGCGACATATGGAAAGGTTCTTGTCTTAGATGGGGTGATTCAGTTAACTGAAAGGGATGAATGTGCCTATCAAGAAATGATCACTCATCTCCCACTCTGCTCAATTCCAAACCCAAAGAAGGTTTTGGTTATTGGTGGGGGAGATGGTGGGGTACTGCGGGAAGTGGCTCGCTATGCTTCAGTTGAGAAGATTGATATATGTGAGATCGATAAGATGGTGGTTGATGTGTCTAAACAGTTTTTCCCTGATGTAGCTATTGGATACGATGACCCACGGGTCTCACTTCATGTTGGTGATGGAGTTGCGTTTTTGAAGGCCGTTCCTGCAGGAACTTATGATGCAGTAATAGTGGACTCTTCTGACCCAATAGGTCCTGCGAAAGAACTTTTTGAGAAGCCCTTTTTCCAGACAGTAGCTAATGCTCTTCGTCCAGGAGGAGTTGTGTGTACACAGGCAGAAAGTATATGGCTTCACATGGACATCATTGAGGATATTGTGGAAAACTGTCGTGAGGTATTCAAAGGCTCTGTCAACTATGCATGGACTACAGTCCCTACATATCCAAGTGGGGTGATTGGTTTTATGCTTTGTTCTACCGAGGGACCTGCTGTGGATTTTAAGCACCCTGTGAATTCAATTGATGCATCAAAACGACCCTTAAAATTTTACAACTCAGAGATTCATACAGCTGCTTTCTGTCTACCATCTTTTGCGAAGAAGGTGATTGACGCAAAAGCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004766 GO:0005575 GO:0005623 GO:0005886 GO:0006576 GO:0006595 GO:0006596 GO:0006807 GO:0008150 GO:0008152 GO:0008216 GO:0008295 GO:0009058 GO:0009308 GO:0009309 GO:0009987 GO:0016020 GO:0016740 GO:0016765 GO:0034641 GO:0042401 GO:0044106 GO:0044237 GO:0044249 GO:0044271 GO:0044464 GO:0071704 GO:0071944 GO:0097164 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

336

Amino Acids

36.39

Weight (kDa)

4.79

Isoelectric Point (pI)

38.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	51 - 105	2.3e-24	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	108 - 295	1.5e-79	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005047)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23820 AT1G23820 AT1G70310
fragaria_vesca	FvH4_4g34680
malus_domestica	MD13G1090400.v1.1 MD14G1183600.v1.1 MD16G1091000.v1.1
prunus_persica	Prupe.1G255300_v2.0.a1 Prupe.5G176100_v2.0.a1
pyrus_communis	pycom13g07870 pycom16g07770
rosa_chinensis	RchiOBHm_Chr4g0444031 RchiOBHm_Chr7g0193881
rosa_laevigata	RLG00000004238 RLG00000005863
rosa_multiflora	Rmu_sc0015522.1_g000006
rosa_roxburghii	Rroxscaffold_3G00261760 Rroxscaffold_5G00384600
rosa_rugosa	Rorug04G0349100 Rorug07G0012500
rosa_samantha	Rh4AG409900 Rh4BG421000 Rh4CG435300 Rh4DG416200 Rh7AG139200 Rh7BG139600 Rh7CG143100 Rh7DG140700
rosa_wichuraiana	Rw4G035210 Rw7G011880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	539
Acc36I	ACCTGC	1 cut(s)	877
AccI	GTMKAC	1 cut(s)	964
AccII	CGCG	1 cut(s)	74
AciI	CCGC	4 cut(s)	5, 74, 138, 421
AcoI	YGGCCR	1 cut(s)	179
AcsI	RAATTY	2 cut(s)	895, 926
AcuI	CTGAAG	2 cut(s)	72, 426
AdeI	CACNNNGTG	1 cut(s)	892
AfaI	GTAC	2 cut(s)	417, 712
AfiI	CCNNNNNNNGG	2 cut(s)	45, 382
AgsI	TTSAA	6 cut(s)	43, 202, 224, 577, 784, 900
AjnI	CCWGG	3 cut(s)	50, 181, 694
AluBI	AGCT	4 cut(s)	80, 518, 680, 953
AluI	AGCT	4 cut(s)	80, 518, 680, 953
Alw26I	GTCTC	3 cut(s)	98, 139, 547
AoxI	GGCC	3 cut(s)	47, 179, 579
ApeKI	GCWGC	3 cut(s)	80, 83, 953
ApoI	RAATTY	2 cut(s)	895, 926
ArsI	GACNNNNNNTTYG	2 cut(s)	777, 809
AspLEI	GCGC	1 cut(s)	74
AspS9I	GGNCC	3 cut(s)	48, 635, 866
AsuC2I	CCSGG	1 cut(s)	150
AsuHPI	GGTGA	4 cut(s)	310, 569, 847, 997
AvaII	GGWCC	2 cut(s)	635, 866
BalI	TGGCCA	1 cut(s)	181
BbsI	GAAGAC	1 cut(s)	247
BbvI	GCAGC	3 cut(s)	67, 70, 940
BccI	CCATC	6 cut(s)	244, 287, 401, 472, 554, 976
BceAI	ACGGC	3 cut(s)	77, 85, 566
BcgI	CGANNNNNNTGC	2 cut(s)	851, 885
BciT130I	CCWGG	3 cut(s)	52, 183, 696
BciVI	GTATCC	1 cut(s)	518
BclI	TGATCA	1 cut(s)	342
BcnI	CCSGG	1 cut(s)	150
BcoDI	GTCTC	3 cut(s)	98, 139, 547
BfmI	CTRYAG	2 cut(s)	588, 810
BfuAI	ACCTGC	1 cut(s)	877
BfuI	GTATCC	1 cut(s)	518
BisI	GCNGC	3 cut(s)	81, 84, 954
BlsI	GCNGC	3 cut(s)	82, 85, 955
Bme1390I	CCNGG	4 cut(s)	52, 150, 183, 696
Bme18I	GGWCC	2 cut(s)	635, 866
BmgT120I	GGNCC	3 cut(s)	48, 635, 866
BmiI	GGNNCC	2 cut(s)	123, 867
BmrFI	CCNGG	4 cut(s)	52, 150, 183, 696
BmsI	GCATC	3 cut(s)	592, 895, 917
BoxI	GACNNNNGTC	1 cut(s)	812
BpiI	GAAGAC	1 cut(s)	247
BplI	GAGNNNNNCTC	2 cut(s)	179, 211
BpuMI	CCSGG	1 cut(s)	150
Bsa29I	ATCGAT	1 cut(s)	471
BsaI	GGTCTC	1 cut(s)	547
BsaJI	CCNNGG	5 cut(s)	50, 51, 99, 536, 861
BsaXI	ACNNNNNCTCC	2 cut(s)	690, 720
Bsc4I	CCNNNNNNNGG	2 cut(s)	45, 382
Bse1I	ACTGG	1 cut(s)	259
BseBI	CCWGG	3 cut(s)	52, 183, 696
BseCI	ATCGAT	1 cut(s)	471
BseDI	CCNNGG	5 cut(s)	50, 51, 99, 536, 861
BseGI	GGATG	1 cut(s)	325
BseLI	CCNNNNNNNGG	2 cut(s)	45, 382
BseMII	CTCAG	2 cut(s)	153, 951
BseNI	ACTGG	1 cut(s)	259
BseRI	GAGGAG	1 cut(s)	714
BseXI	GCAGC	3 cut(s)	67, 70, 940
Bsh1236I	CGCG	1 cut(s)	74
BshFI	GGCC	3 cut(s)	49, 181, 581
BshVI	ATCGAT	1 cut(s)	471
BsiSI	CCGG	2 cut(s)	125, 150
BslFI	GGGAC	2 cut(s)	800, 879
BslI	CCNNNNNNNGG	2 cut(s)	45, 382
BsmAI	GTCTC	3 cut(s)	98, 139, 547
BsmFI	GGGAC	2 cut(s)	800, 879
BsnI	GGCC	3 cut(s)	49, 181, 581
Bso31I	GGTCTC	1 cut(s)	547
Bsp1407I	TGTACA	1 cut(s)	710
Bsp143I	GATC	2 cut(s)	342, 468
Bsp19I	CCATGG	1 cut(s)	99
BspACI	CCGC	4 cut(s)	5, 74, 138, 421
BspANI	GGCC	3 cut(s)	49, 181, 581
BspCNI	CTCAG	2 cut(s)	154, 950
BspDI	ATCGAT	1 cut(s)	471
BspFNI	CGCG	1 cut(s)	74
BspLI	GGNNCC	2 cut(s)	123, 867
BspMAI	CTGCAG	1 cut(s)	592
BspMI	ACCTGC	1 cut(s)	877
BspQI	GCTCTTC	1 cut(s)	693
BspTNI	GGTCTC	1 cut(s)	547
BsrGI	TGTACA	1 cut(s)	710
BsrI	ACTGG	1 cut(s)	259
BssECI	CCNNGG	5 cut(s)	50, 51, 99, 536, 861
BssMI	GATC	2 cut(s)	342, 468
BssT1I	CCWWGG	1 cut(s)	99
Bst2UI	CCWGG	3 cut(s)	52, 183, 696
Bst4CI	ACNGT	5 cut(s)	113, 501, 676, 770, 814
Bst6I	CTCTTC	4 cut(s)	38, 57, 625, 693
BstAUI	TGTACA	1 cut(s)	710
BstC8I	GCNNGC	1 cut(s)	433
BstDEI	CTNAG	3 cut(s)	162, 289, 937
BstDSI	CCRYGG	2 cut(s)	99, 536
BstENI	CCTNNNNNAGG	1 cut(s)	43
BstF5I	GGATG	1 cut(s)	325
BstFNI	CGCG	1 cut(s)	74
BstHHI	GCGC	1 cut(s)	74
BstKTI	GATC	2 cut(s)	345, 471
BstMAI	GTCTC	3 cut(s)	98, 139, 547
BstMBI	GATC	2 cut(s)	342, 468
BstMWI	GCNNNNNNNGC	4 cut(s)	80, 89, 437, 587
BstNI	CCWGG	3 cut(s)	52, 183, 696
BstPAI	GACNNNNGTC	1 cut(s)	812
BstSCI	CCNGG	4 cut(s)	50, 148, 181, 694
BstSFI	CTRYAG	2 cut(s)	588, 810
BstUI	CGCG	1 cut(s)	74
BstV1I	GCAGC	3 cut(s)	67, 70, 940
BstV2I	GAAGAC	1 cut(s)	247
Bsu15I	ATCGAT	1 cut(s)	471
BsuI	GTATCC	1 cut(s)	518
BsuRI	GGCC	3 cut(s)	49, 181, 581
BsuTUI	ATCGAT	1 cut(s)	471
BtgI	CCRYGG	2 cut(s)	99, 536
BtsCI	GGATG	1 cut(s)	325
BveI	ACCTGC	1 cut(s)	877
Cac8I	GCNNGC	1 cut(s)	433
CfoI	GCGC	1 cut(s)	74
Cfr13I	GGNCC	3 cut(s)	48, 635, 866
ClaI	ATCGAT	1 cut(s)	471
CseI	GACGC	1 cut(s)	1001
Csp6I	GTAC	2 cut(s)	416, 711
CviAII	CATG	4 cut(s)	100, 551, 739, 804
CviQI	GTAC	2 cut(s)	416, 711
DdeI	CTNAG	3 cut(s)	162, 289, 937
DpnI	GATC	2 cut(s)	344, 470
DpnII	GATC	2 cut(s)	342, 468
DraIII	CACNNNGTG	1 cut(s)	892
DrdI	GACNNNNNNGTC	1 cut(s)	539
DseDI	GACNNNNNNGTC	1 cut(s)	539
EaeI	YGGCCR	1 cut(s)	179
Eam1104I	CTCTTC	4 cut(s)	38, 57, 625, 693
EarI	CTCTTC	4 cut(s)	38, 57, 625, 693
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	1 cut(s)	99
Eco31I	GGTCTC	1 cut(s)	547
Eco47I	GGWCC	2 cut(s)	635, 866
Eco57I	CTGAAG	2 cut(s)	72, 426
EcoNI	CCTNNNNNAGG	1 cut(s)	43
EcoO109I	RGGNCCY	2 cut(s)	635, 866
EcoRI	GAATTC	1 cut(s)	895
EcoRII	CCWGG	3 cut(s)	50, 181, 694
EcoT14I	CCWWGG	1 cut(s)	99
EcoT22I	ATGCAT	2 cut(s)	805, 910
ErhI	CCWWGG	1 cut(s)	99
FaeI	CATG	4 cut(s)	103, 554, 742, 807
FaqI	GGGAC	2 cut(s)	800, 879
FatI	CATG	4 cut(s)	99, 550, 738, 803
FauI	CCCGC	1 cut(s)	414
FauNDI	CATATG	1 cut(s)	271
FbaI	TGATCA	1 cut(s)	342
FblI	GTMKAC	1 cut(s)	964
Fnu4HI	GCNGC	3 cut(s)	81, 84, 954
FokI	GGATG	1 cut(s)	332
Fsp4HI	GCNGC	3 cut(s)	81, 84, 954
GlaI	GCGC	1 cut(s)	73
GluI	GCNGC	3 cut(s)	81, 84, 954
HaeIII	GGCC	3 cut(s)	49, 181, 581
HapII	CCGG	2 cut(s)	125, 150
HgaI	GACGC	1 cut(s)	1001
HhaI	GCGC	1 cut(s)	74
Hin1II	CATG	4 cut(s)	103, 554, 742, 807
Hin6I	GCGC	1 cut(s)	72
HinP1I	GCGC	1 cut(s)	72
HincII	GTYRAC	2 cut(s)	309, 796
HindII	GTYRAC	2 cut(s)	309, 796
HinfI	GANTC	4 cut(s)	128, 301, 617, 943
HpaI	GTTAAC	1 cut(s)	309
HpaII	CCGG	2 cut(s)	125, 150
HphI	GGTGA	4 cut(s)	310, 569, 847, 997
Hpy166II	GTNNAC	6 cut(s)	309, 616, 711, 713, 796, 965
Hpy188I	TCNGA	5 cut(s)	163, 235, 243, 625, 940
Hpy188III	TCNNGA	3 cut(s)	335, 670, 773
Hpy8I	GTNNAC	6 cut(s)	309, 616, 711, 713, 796, 965
HpyAV	CCTTC	3 cut(s)	376, 571, 976
HpyCH4III	ACNGT	5 cut(s)	113, 501, 676, 770, 814
HpyCH4V	TGCA	4 cut(s)	590, 605, 803, 908
HpyF10VI	GCNNNNNNNGC	4 cut(s)	80, 89, 437, 587
HpyF3I	CTNAG	3 cut(s)	162, 289, 937
Hsp92II	CATG	4 cut(s)	103, 554, 742, 807
HspAI	GCGC	1 cut(s)	72
Ksp22I	TGATCA	1 cut(s)	342
KspAI	GTTAAC	1 cut(s)	309
Kzo9I	GATC	2 cut(s)	342, 468
LguI	GCTCTTC	1 cut(s)	693
LmnI	GCTCC	1 cut(s)	121
Lsp1109I	GCAGC	3 cut(s)	67, 70, 940
LweI	GCATC	3 cut(s)	592, 895, 917
MalI	GATC	2 cut(s)	344, 470
MboI	GATC	2 cut(s)	342, 468
MfeI	CAATTG	1 cut(s)	900
MlsI	TGGCCA	1 cut(s)	181
MluCI	AATT	4 cut(s)	366, 895, 900, 926
MluNI	TGGCCA	1 cut(s)	181
MlyI	GAGTC	1 cut(s)	611
MmeI	TCCRAC	1 cut(s)	258
MnlI	CCTC	7 cut(s)	39, 49, 181, 692, 745, 769, 856
Mox20I	TGGCCA	1 cut(s)	181
Mph1103I	ATGCAT	2 cut(s)	805, 910
MscI	TGGCCA	1 cut(s)	181
MseI	TTAA	3 cut(s)	308, 882, 923
MslI	CAYNNNNRTG	1 cut(s)	555
Msp20I	TGGCCA	1 cut(s)	181
MspA1I	CMGCKG	2 cut(s)	140, 953
MspI	CCGG	2 cut(s)	125, 150
MspR9I	CCNGG	4 cut(s)	52, 150, 183, 696
MunI	CAATTG	1 cut(s)	900
MvaI	CCWGG	3 cut(s)	52, 183, 696
MvnI	CGCG	1 cut(s)	74
MwoI	GCNNNNNNNGC	4 cut(s)	80, 89, 437, 587
NciI	CCSGG	1 cut(s)	150
NcoI	CCATGG	1 cut(s)	99
NdeI	CATATG	1 cut(s)	271
NdeII	GATC	2 cut(s)	342, 468
NlaIII	CATG	4 cut(s)	103, 554, 742, 807
NlaIV	GGNNCC	2 cut(s)	123, 867
NsiI	ATGCAT	2 cut(s)	805, 910
PasI	CCCWGGG	1 cut(s)	51
PciSI	GCTCTTC	1 cut(s)	693
PfeI	GAWTC	3 cut(s)	128, 301, 943
PfoI	TCCNGGA	1 cut(s)	694
PkrI	GCNGC	3 cut(s)	82, 85, 955
PleI	GAGTC	1 cut(s)	611
PpsI	GAGTC	1 cut(s)	611
PpuMI	RGGWCCY	2 cut(s)	635, 866
PshAI	GACNNNNGTC	1 cut(s)	812
Psp5II	RGGWCCY	2 cut(s)	635, 866
Psp6I	CCWGG	3 cut(s)	50, 181, 694
PspGI	CCWGG	3 cut(s)	50, 181, 694
PspN4I	GGNNCC	2 cut(s)	123, 867
PspPI	GGNCC	3 cut(s)	48, 635, 866
PspPPI	RGGWCCY	2 cut(s)	635, 866
PstI	CTGCAG	1 cut(s)	592
PvuII	CAGCTG	1 cut(s)	953
RsaI	GTAC	2 cut(s)	417, 712
RsaNI	GTAC	2 cut(s)	416, 711
RseI	CAYNNNNRTG	1 cut(s)	555
SapI	GCTCTTC	1 cut(s)	693
SaqAI	TTAA	3 cut(s)	308, 882, 923
SatI	GCNGC	3 cut(s)	81, 84, 954
Sau3AI	GATC	2 cut(s)	342, 468
Sau96I	GGNCC	3 cut(s)	48, 635, 866
SbfI	CCTGCAGG	1 cut(s)	592
SchI	GAGTC	1 cut(s)	611
ScrFI	CCNGG	4 cut(s)	52, 150, 183, 696
SdaI	CCTGCAGG	1 cut(s)	592
SfaNI	GCATC	3 cut(s)	592, 895, 917
SfcI	CTRYAG	2 cut(s)	588, 810
SinI	GGWCC	2 cut(s)	635, 866
SmiMI	CAYNNNNRTG	1 cut(s)	555
Sse8387I	CCTGCAGG	1 cut(s)	592
Sse9I	AATT	4 cut(s)	366, 895, 900, 926
SsiI	CCGC	4 cut(s)	5, 74, 138, 421
StyD4I	CCNGG	4 cut(s)	50, 148, 181, 694
StyI	CCWWGG	1 cut(s)	99
TaaI	ACNGT	5 cut(s)	113, 501, 676, 770, 814
TaqI	TCGA	1 cut(s)	471
TasI	AATT	4 cut(s)	366, 895, 900, 926
TatI	WGTACW	1 cut(s)	710
TfiI	GAWTC	3 cut(s)	128, 301, 943
Tru1I	TTAA	3 cut(s)	308, 882, 923
Tru9I	TTAA	3 cut(s)	308, 882, 923
TseI	GCWGC	3 cut(s)	80, 83, 953
TspDTI	ATGAA	3 cut(s)	336, 539, 935
VpaK11BI	GGWCC	2 cut(s)	635, 866
XagI	CCTNNNNNAGG	1 cut(s)	43
XapI	RAATTY	2 cut(s)	895, 926
XmiI	GTMKAC	1 cut(s)	964
Zsp2I	ATGCAT	2 cut(s)	805, 910

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.