Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0005047

Physical Location & Seq

Genomic Coordinates Forward (+)

62020241 .. 62023165

Gene Structure 2925 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G035210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1011 bp

ATGGCCGAAGACGGTGTGGTGGGGTCCGCTGATTTTCCGGCGAAGCGGCCCAGAGAGGAGGAAGAGAACTGTGTCTCGTCAGCTGCGGCGGCTATGGAGACCGAGACTGCCAAAGACCCGGATTGCGTCTCTGGTGTCATTCCTGGCTGGTTCTCTGAGATTAGCCCAATGTGGCCCGGAGAAGCACACTCCTTGAAGATAGAGAAAATTTTGTTTCAAGGGAAATCGGACTATCAGAATGTGATGGTCTTCCAGTCATCAACCTATGGAAAGGTTCTCGTCCTGGATGGGGTGATTCAGCTGACCGAAAGGGATGAATGTGCCTATCAAGAAATGATTACTCATCTTCCTCTCTGCTCAATTCCAAACCCCAAGAAGGTTTTGGTTATCGGTGGTGGAGACGGTGGTGTACTGCGGGAAGTGGCTCGCCATTCTTCTGTTGAGCAAATAGATATATGTGAGATTGATAAGATGGTTGTTGACGTTTCGAAACAGTTTTTCCCTCGTGTGGCTATTGGATATGAGGATCCCAGGGTGACACTCAATGTTGGTGATGGAGTCGCATTTTTAAGGGCTGTTCCTGCAGGAACTTATGATGCAATAATTGTGGACTCTTCTGATCCCATTGGTCCTGCACAAGAGCTTTTTGAGAAGCCCTTCTTTCAGTTGGTAGCCAACGCTCTCCGTCCAGGAGGAGTTGTGTGCACCCAGGCAGAGAGTATATGGCTTCACATGCACATCATTGAGGATATTGTGTCAAACTGCCACCAGATATTCAAAGGCTCTGTCAACTACGCATGGACAACAGTCCCTACATATCCAAGTGGAGTGATTGGTTTTATGCTTTGTTCGACCGAGGGTCCTCCTGTTGATTTTAAGCACCCTGTGAATCCAATCGCTGAAAATGACGGTTCCAAAGACCCCTTGAAATTTTACAACTCTGAGATTCACACAGCTGCTTTCTGTCTACCATCTTTTGCAAAGAAGGTGATTGAATCAATGGCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004766 GO:0005575 GO:0005623 GO:0005886 GO:0006576 GO:0006595 GO:0006596 GO:0006807 GO:0008150 GO:0008152 GO:0008216 GO:0008295 GO:0009058 GO:0009308 GO:0009309 GO:0009987 GO:0016020 GO:0016740 GO:0016765 GO:0034641 GO:0042401 GO:0044106 GO:0044237 GO:0044249 GO:0044271 GO:0044464 GO:0071704 GO:0071944 GO:0097164 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

336

Amino Acids

36.64

Weight (kDa)

4.82

Isoelectric Point (pI)

47.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	49 - 103	3.1e-24	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	106 - 293	9.9e-79	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005047)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23820 AT1G23820 AT1G70310
fragaria_vesca	FvH4_4g34680
malus_domestica	MD13G1090400.v1.1 MD14G1183600.v1.1 MD16G1091000.v1.1
prunus_persica	Prupe.1G255300_v2.0.a1 Prupe.5G176100_v2.0.a1
pyrus_communis	pycom13g07870 pycom16g07770
rosa_chinensis	RchiOBHm_Chr4g0444031 RchiOBHm_Chr7g0193881
rosa_laevigata	RLG00000004238 RLG00000005863
rosa_multiflora	Rmu_sc0015522.1_g000006
rosa_roxburghii	Rroxscaffold_3G00261760 Rroxscaffold_5G00384600
rosa_rugosa	Rorug04G0349100 Rorug07G0012500
rosa_samantha	Rh4AG409900 Rh4BG421000 Rh4CG435300 Rh4DG416200 Rh7AG139200 Rh7BG139600 Rh7CG143100 Rh7DG140700
rosa_wichuraiana	Rw4G035210 Rw7G011880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	967
AciI	CCGC	5 cut(s)	27, 46, 86, 89, 415
AclWI	GGATC	3 cut(s)	521, 534, 614
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	207, 929
AdeI	CACNNNGTG	1 cut(s)	886
AfaI	GTAC	1 cut(s)	411
AfiI	CCNNNNNNNGG	3 cut(s)	289, 376, 508
AgsI	TTSAA	5 cut(s)	196, 218, 778, 928, 995
AhdI	GACNNNNNGTC	1 cut(s)	858
AjnI	CCWGG	5 cut(s)	142, 282, 530, 688, 708
AluBI	AGCT	4 cut(s)	83, 301, 643, 956
AluI	AGCT	4 cut(s)	83, 301, 643, 956
Alw21I	GWGCWC	1 cut(s)	707
Alw26I	GTCTC	5 cut(s)	79, 92, 98, 133, 393
Alw44I	GTGCAC	1 cut(s)	703
AlwI	GGATC	3 cut(s)	521, 534, 614
AoxI	GGCC	3 cut(s)	3, 47, 173
ApaLI	GTGCAC	1 cut(s)	703
ApeKI	GCWGC	2 cut(s)	83, 956
ApoI	RAATTY	2 cut(s)	207, 929
ArsI	GACNNNNNNTTYG	2 cut(s)	771, 803
Asp700I	GAANNNNTTC	1 cut(s)	656
AspS9I	GGNCC	5 cut(s)	24, 48, 174, 629, 860
AsuC2I	CCSGG	2 cut(s)	119, 177
AsuHPI	GGTGA	4 cut(s)	304, 547, 563, 1000
AsuII	TTCGAA	1 cut(s)	488
AvaII	GGWCC	3 cut(s)	24, 629, 860
BaeGI	GKGCMC	1 cut(s)	707
BamHI	GGATCC	1 cut(s)	526
BauI	CACGAG	1 cut(s)	504
BbsI	GAAGAC	2 cut(s)	15, 241
Bbv12I	GWGCWC	1 cut(s)	707
BbvI	GCAGC	2 cut(s)	70, 943
BccI	CCATC	5 cut(s)	238, 281, 466, 548, 979
BciT130I	CCWGG	5 cut(s)	144, 284, 532, 690, 710
BcnI	CCSGG	2 cut(s)	119, 177
BcoDI	GTCTC	5 cut(s)	79, 92, 98, 133, 393
BfmI	CTRYAG	1 cut(s)	582
BisI	GCNGC	5 cut(s)	47, 84, 87, 90, 957
BlsI	GCNGC	5 cut(s)	48, 85, 88, 91, 958
Bme1390I	CCNGG	7 cut(s)	119, 144, 177, 284, 532, 690, 710
Bme18I	GGWCC	3 cut(s)	24, 629, 860
BmeRI	GACNNNNNGTC	1 cut(s)	858
BmgT120I	GGNCC	5 cut(s)	24, 48, 174, 629, 860
BmiI	GGNNCC	4 cut(s)	25, 528, 861, 913
BmrFI	CCNGG	7 cut(s)	119, 144, 177, 284, 532, 690, 710
BmsI	GCATC	1 cut(s)	586
BoxI	GACNNNNGTC	1 cut(s)	806
BpiI	GAAGAC	2 cut(s)	15, 241
Bpu14I	TTCGAA	1 cut(s)	488
BpuMI	CCSGG	2 cut(s)	119, 177
BsaI	GGTCTC	1 cut(s)	92
BsaJI	CCNNGG	4 cut(s)	530, 531, 708, 855
BsaXI	ACNNNNNCTCC	2 cut(s)	684, 714
Bsc4I	CCNNNNNNNGG	3 cut(s)	289, 376, 508
Bse1I	ACTGG	1 cut(s)	253
BseBI	CCWGG	5 cut(s)	144, 284, 532, 690, 710
BseDI	CCNNGG	4 cut(s)	530, 531, 708, 855
BseGI	GGATG	2 cut(s)	292, 319
BseLI	CCNNNNNNNGG	3 cut(s)	289, 376, 508
BseMII	CTCAG	2 cut(s)	147, 933
BseNI	ACTGG	1 cut(s)	253
BseRI	GAGGAG	2 cut(s)	71, 708
BseSI	GKGCMC	1 cut(s)	707
BseXI	GCAGC	2 cut(s)	70, 943
BsgI	GTGCAG	1 cut(s)	618
Bsh1285I	CGRYCG	1 cut(s)	855
BshFI	GGCC	3 cut(s)	5, 49, 175
BsiEI	CGRYCG	1 cut(s)	855
BsiHKAI	GWGCWC	1 cut(s)	707
BsiSI	CCGG	3 cut(s)	38, 119, 177
BslFI	GGGAC	1 cut(s)	794
BslI	CCNNNNNNNGG	3 cut(s)	289, 376, 508
BsmAI	GTCTC	5 cut(s)	79, 92, 98, 133, 393
BsmBI	CGTCTC	2 cut(s)	133, 393
BsmFI	GGGAC	1 cut(s)	794
BsnI	GGCC	3 cut(s)	5, 49, 175
Bso31I	GGTCTC	1 cut(s)	92
Bsp119I	TTCGAA	1 cut(s)	488
Bsp1286I	GDGCHC	1 cut(s)	707
Bsp143I	GATC	2 cut(s)	526, 619
BspACI	CCGC	5 cut(s)	27, 46, 86, 89, 415
BspANI	GGCC	3 cut(s)	5, 49, 175
BspCNI	CTCAG	2 cut(s)	148, 934
BspLI	GGNNCC	4 cut(s)	25, 528, 861, 913
BspMAI	CTGCAG	1 cut(s)	586
BspPI	GGATC	3 cut(s)	521, 534, 614
BspT104I	TTCGAA	1 cut(s)	488
BspTNI	GGTCTC	1 cut(s)	92
BsrI	ACTGG	1 cut(s)	253
BssECI	CCNNGG	4 cut(s)	530, 531, 708, 855
BssMI	GATC	2 cut(s)	526, 619
BssSI	CACGAG	1 cut(s)	504
Bst2BI	CACGAG	1 cut(s)	504
Bst2UI	CCWGG	5 cut(s)	144, 284, 532, 690, 710
Bst4CI	ACNGT	6 cut(s)	14, 71, 404, 495, 808, 911
Bst6I	CTCTTC	2 cut(s)	57, 619
BstBI	TTCGAA	1 cut(s)	488
BstC8I	GCNNGC	1 cut(s)	427
BstDEI	CTNAG	2 cut(s)	156, 942
BstF5I	GGATG	2 cut(s)	292, 319
BstKTI	GATC	2 cut(s)	529, 622
BstMAI	GTCTC	5 cut(s)	79, 92, 98, 133, 393
BstMBI	GATC	2 cut(s)	526, 619
BstMCI	CGRYCG	1 cut(s)	855
BstMWI	GCNNNNNNNGC	3 cut(s)	89, 581, 733
BstNI	CCWGG	5 cut(s)	144, 284, 532, 690, 710
BstNSI	RCATGY	1 cut(s)	736
BstPAI	GACNNNNGTC	1 cut(s)	806
BstSCI	CCNGG	7 cut(s)	117, 142, 175, 282, 530, 688, 708
BstSFI	CTRYAG	1 cut(s)	582
BstSLI	GKGCMC	1 cut(s)	707
BstV1I	GCAGC	2 cut(s)	70, 943
BstV2I	GAAGAC	2 cut(s)	15, 241
BstX2I	RGATCY	1 cut(s)	526
BstYI	RGATCY	1 cut(s)	526
BsuRI	GGCC	3 cut(s)	5, 49, 175
BtsCI	GGATG	2 cut(s)	292, 319
Cac8I	GCNNGC	1 cut(s)	427
Cfr13I	GGNCC	5 cut(s)	24, 48, 174, 629, 860
CseI	GACGC	1 cut(s)	115
Csp6I	GTAC	1 cut(s)	410
CspCI	CAANNNNNGTGG	2 cut(s)	588, 623
CviAII	CATG	2 cut(s)	733, 798
CviQI	GTAC	1 cut(s)	410
DdeI	CTNAG	2 cut(s)	156, 942
DpnI	GATC	2 cut(s)	528, 621
DpnII	GATC	2 cut(s)	526, 619
DraIII	CACNNNGTG	1 cut(s)	886
DriI	GACNNNNNGTC	1 cut(s)	858
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	57, 619
Eam1105I	GACNNNNNGTC	1 cut(s)	858
EarI	CTCTTC	2 cut(s)	57, 619
Eco31I	GGTCTC	1 cut(s)	92
Eco47I	GGWCC	3 cut(s)	24, 629, 860
EcoO109I	RGGNCCY	1 cut(s)	860
EcoRII	CCWGG	5 cut(s)	142, 282, 530, 688, 708
Esp3I	CGTCTC	2 cut(s)	133, 393
FaeI	CATG	2 cut(s)	736, 801
FalI	AAGNNNNNCTT	2 cut(s)	644, 676
FaqI	GGGAC	1 cut(s)	794
FatI	CATG	2 cut(s)	732, 797
FauI	CCCGC	1 cut(s)	408
FblI	GTMKAC	1 cut(s)	967
Fnu4HI	GCNGC	5 cut(s)	47, 84, 87, 90, 957
FokI	GGATG	2 cut(s)	299, 326
Fsp4HI	GCNGC	5 cut(s)	47, 84, 87, 90, 957
GluI	GCNGC	5 cut(s)	47, 84, 87, 90, 957
HaeIII	GGCC	3 cut(s)	5, 49, 175
HapII	CCGG	3 cut(s)	38, 119, 177
HgaI	GACGC	1 cut(s)	115
Hin1II	CATG	2 cut(s)	736, 801
HincII	GTYRAC	2 cut(s)	481, 790
HindII	GTYRAC	2 cut(s)	481, 790
HinfI	GANTC	6 cut(s)	295, 558, 611, 889, 946, 995
HpaII	CCGG	3 cut(s)	38, 119, 177
HphI	GGTGA	4 cut(s)	304, 547, 563, 1000
Hpy166II	GTNNAC	6 cut(s)	410, 481, 610, 705, 790, 968
Hpy188I	TCNGA	5 cut(s)	157, 229, 237, 619, 943
Hpy188III	TCNNGA	1 cut(s)	329
Hpy8I	GTNNAC	6 cut(s)	410, 481, 610, 705, 790, 968
HpyAV	CCTTC	3 cut(s)	370, 667, 979
HpyCH4III	ACNGT	6 cut(s)	14, 71, 404, 495, 808, 911
HpyCH4IV	ACGT	1 cut(s)	483
HpyCH4V	TGCA	6 cut(s)	584, 599, 635, 705, 736, 980
HpyF10VI	GCNNNNNNNGC	3 cut(s)	89, 581, 733
HpyF3I	CTNAG	2 cut(s)	156, 942
HpySE526I	ACGT	1 cut(s)	483
Hsp92II	CATG	2 cut(s)	736, 801
Kzo9I	GATC	2 cut(s)	526, 619
Lsp1109I	GCAGC	2 cut(s)	70, 943
LweI	GCATC	1 cut(s)	586
MaeII	ACGT	1 cut(s)	483
MaeIII	GTNAC	1 cut(s)	535
MalI	GATC	2 cut(s)	528, 621
MboI	GATC	2 cut(s)	526, 619
MboII	GAAGA	7 cut(s)	20, 74, 208, 241, 338, 426, 606
MflI	RGATCY	1 cut(s)	526
MhlI	GDGCHC	1 cut(s)	707
MluCI	AATT	4 cut(s)	207, 360, 603, 929
MlyI	GAGTC	2 cut(s)	567, 605
MnlI	CCTC	9 cut(s)	49, 52, 360, 513, 517, 686, 739, 850, 873
MroXI	GAANNNNTTC	1 cut(s)	656
MseI	TTAA	2 cut(s)	569, 876
MspA1I	CMGCKG	4 cut(s)	29, 83, 301, 956
MspI	CCGG	3 cut(s)	38, 119, 177
MspR9I	CCNGG	7 cut(s)	119, 144, 177, 284, 532, 690, 710
MvaI	CCWGG	5 cut(s)	144, 284, 532, 690, 710
MwoI	GCNNNNNNNGC	3 cut(s)	89, 581, 733
NciI	CCSGG	2 cut(s)	119, 177
NdeII	GATC	2 cut(s)	526, 619
NlaIII	CATG	2 cut(s)	736, 801
NlaIV	GGNNCC	4 cut(s)	25, 528, 861, 913
NmuCI	GTSAC	1 cut(s)	535
NspI	RCATGY	1 cut(s)	736
NspV	TTCGAA	1 cut(s)	488
PasI	CCCWGGG	1 cut(s)	531
PdmI	GAANNNNTTC	1 cut(s)	656
PfeI	GAWTC	4 cut(s)	295, 889, 946, 995
PfoI	TCCNGGA	2 cut(s)	282, 688
PkrI	GCNGC	5 cut(s)	48, 85, 88, 91, 958
PleI	GAGTC	2 cut(s)	566, 605
PpsI	GAGTC	2 cut(s)	566, 605
PpuMI	RGGWCCY	1 cut(s)	860
PshAI	GACNNNNGTC	1 cut(s)	806
Psp5II	RGGWCCY	1 cut(s)	860
Psp6I	CCWGG	5 cut(s)	142, 282, 530, 688, 708
PspGI	CCWGG	5 cut(s)	142, 282, 530, 688, 708
PspN4I	GGNNCC	4 cut(s)	25, 528, 861, 913
PspPI	GGNCC	5 cut(s)	24, 48, 174, 629, 860
PspPPI	RGGWCCY	1 cut(s)	860
PstI	CTGCAG	1 cut(s)	586
PsuI	RGATCY	1 cut(s)	526
PvuII	CAGCTG	3 cut(s)	83, 301, 956
RsaI	GTAC	1 cut(s)	411
RsaNI	GTAC	1 cut(s)	410
SaqAI	TTAA	2 cut(s)	569, 876
SatI	GCNGC	5 cut(s)	47, 84, 87, 90, 957
Sau3AI	GATC	2 cut(s)	526, 619
Sau96I	GGNCC	5 cut(s)	24, 48, 174, 629, 860
SbfI	CCTGCAGG	1 cut(s)	586
SchI	GAGTC	2 cut(s)	567, 605
ScrFI	CCNGG	7 cut(s)	119, 144, 177, 284, 532, 690, 710
SdaI	CCTGCAGG	1 cut(s)	586
SduI	GDGCHC	1 cut(s)	707
SetI	ASST	9 cut(s)	85, 266, 276, 303, 381, 486, 645, 958, 990
SfaNI	GCATC	1 cut(s)	586
SfcI	CTRYAG	1 cut(s)	582
SfuI	TTCGAA	1 cut(s)	488
SinI	GGWCC	3 cut(s)	24, 629, 860
Sse8387I	CCTGCAGG	1 cut(s)	586
Sse9I	AATT	4 cut(s)	207, 360, 603, 929
SsiI	CCGC	5 cut(s)	27, 46, 86, 89, 415
StyD4I	CCNGG	7 cut(s)	117, 142, 175, 282, 530, 688, 708
TaaI	ACNGT	6 cut(s)	14, 71, 404, 495, 808, 911
TaiI	ACGT	1 cut(s)	486
TaqI	TCGA	2 cut(s)	488, 851
TaqII	GACCGA	3 cut(s)	116, 320, 869
TasI	AATT	4 cut(s)	207, 360, 603, 929
TatI	WGTACW	1 cut(s)	409
TauI	GCSGC	3 cut(s)	49, 89, 92
TfiI	GAWTC	4 cut(s)	295, 889, 946, 995
Tru1I	TTAA	2 cut(s)	569, 876
Tru9I	TTAA	2 cut(s)	569, 876
TseFI	GTSAC	1 cut(s)	535
TseI	GCWGC	2 cut(s)	83, 956
Tsp45I	GTSAC	1 cut(s)	535
TspDTI	ATGAA	1 cut(s)	330
TspGWI	ACGGA	1 cut(s)	674
VneI	GTGCAC	1 cut(s)	703
VpaK11BI	GGWCC	3 cut(s)	24, 629, 860
XapI	RAATTY	2 cut(s)	207, 929
XceI	RCATGY	1 cut(s)	736
XcmI	CCANNNNNNNNNTGG	1 cut(s)	379
XmiI	GTMKAC	1 cut(s)	967
XmnI	GAANNNNTTC	1 cut(s)	656

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.